Lcy04g005740 (gene) Sponge gourd (P93075) v1

Overview
NameLcy04g005740
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAmino acid/polyamine transporter
LocationChr04: 34537874 .. 34540542 (+)
RNA-Seq ExpressionLcy04g005740
SyntenyLcy04g005740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCACCAAGAATTCAGCCACAGCAAACAACAAGCTTTACAGCTCGAATCCGCCACGGTGGTTGCCGCCTCAGCCCCGCCAACCGCCACGGCCTCCACCGCGGCCACCACTCGAAAGAAGCTAACTTTGCTCCCCCTTGTGTTCCTCATTTACTTCCAAGTTGCTGGTGGTCCTTACGGTGAAGAGCCAGCAGTTCAGGCCGCCGGATCGCTATTCGCGATCATCGGCTTCATCGTCTTCCCATTTTTATGGAGTGTCCCGGAGGCATTGATCACGGCAGAGCTCGCCACTACCTTTCCCGGCAATGGCGGTTTCGTGATATGGGCGGAGCGAGCCTTTGGACCCTTTTGTGGCTCCCTCATGGGCACATGGAAGATCTTTAGCGGAATCATGAACATCACCGCCTTCCCGGCGTTCTTCATCGGCTACGTCGAGAAAATAGTCCCGGCCTTGGAATCCGGCTGGCCTCGCCGCATTGCGGTCTTGGTTTCGACGATACTCCTCTCGCTTCTCAACTACGCCGGGTTGACAATCGTCGGCTACGTCGCCGTTGTTTTGGCTTTCGTTTCGCTCTTACCGTTCATCTTAATGACCTTGATCGCCATGCCGAAGATTCGGGCTCGCCGGTGGTTCAGCTCCGGCGAGAAGGGTGTGAAAAAGGACTGGAATTTGTACCTCAATACCCTCTTTTGGAACCTTAACTTCTGGGACAATGTAAGTACGTTGGCTGGTGAAGTTGAGAACCCACAGAAGACTTACCCCATTGCCCTACTTGTTTCTGTTATATTCATCTCTCTCTCTTACATAATTCCGTTGCTTGCTGTCGTCGGCGCCGTCCCGGTCGAACAAAGCGCGTGGGGGTCGGGGTTTCATGCTCAGGCTGCCGAGTTCATCGGCGGGAATTGGCTGAAAATCCTCCTCGATATCGGTGCAGGTTAGGAGTATTGTTAGAAATATTCACAGCGAGGGACAAAATTATTTCATATTCTAACTATTTGGGTGAATCTTAAATTTTCTAGGGACAAAATAATCATTTTTTGTCTTCAAAGCCTTCAAAATATTTAGGGATTTTTAATCCTAAACTCTGGGGTTGTATCAATTTTAAGTCTGAACTTTACTTTTAATTTCATCAAACTAAAACCTCAAACTTATCACATCGTATCGATTTTAACCTCAAACTTACGTAAATGTTACAATATATACTTTTGGTTAACTTTTCATTTGAACCACCATACGTAATTGATTATTTCTTCCTTTTTTTTTCTTAATTCACATTGCATCTGAATTGGTTTCTTATCAATAAAATATTGGAAAACTTTATTTTAGATGACTTTTTCTTATTGTTGAGAACTTTAATTGTGAGGGTTTTTTCGCTGTTATTTAAGATTATGAAAGCAAATAAACTTTAGAGGTTTGTTATCTTCAAATATTTGTTGATTTTTTATTGTTGCTAAGAACTTTGTGAGATTTTTTTCTTCTTTTACGGTTTTTTTTTCTTGCTAATTAGAAGAATGTACACATGTAGGAAACGAAAATAAAGAAATTTCAATTTTTTAATTTCTCATATTTGTTGGATTTTTCTTGTTGCTAATAATTTTGTGATTCTTTTTTTCATAATAATGAGCAGAGTAGTCTGGGATATTTGATTTCTTCTGTTTTTTCTAAAAAAAATTCTTACAAACTCAAGTGCAATGTGAATTAAAAAAAGGAAAAGGAAATTGTCAATTACATATATGTTTTAATAGCAATTCAAATGAAAAGTTGACCAAAGGTCTACATTGCAACATTTATGCAAGTTTGAGGTTCAAATTGATACAATTTGTTAAGTTTAGAGTTCAATTTCAAGAAATTGAAAGTTTAGGGTTAAAATTGATACAACTTGCTAAGTTCATGAGTCAAAATTGATTTTTTCCCAAATATTTATTTTAGGCTTTGAACTTCCTTAATATATGCTAATCCAATATTTACTTTTAATACAAATCCTGGTTACCAACTGACCAACTTAACATACATGCTCCAAATACAAACTAAGAAAGAAAAGTTTTTAAATTTTTATTCTTTTATGTAATTTTGTAATCTTTCCTTCTTCACTCTGCCCCGATTCAGGGTTGTCAGCAATTGGCATGTTCGAAGCGCAGCTGAGCAGCAGTGCGTATCAAATTCTAGGGATGGCGGAGATCGGGATTCTGCCAAAGTTCTTCGCTTCAAGGGCGAAAGGGTTCGAGACGCCATGGGTGGGGATCTTGATATGCACAGCGGTGTCGCTTGGCGTCTCCTACATGAAGTTCTACGACATCGTGGCGTCAGCGAACTTCATTTACAGCTTGGGAATGTTGCTGGAGTTCGCATCATTCATCTGGTTGAGATGGAAGAAGCCGGAGCTGAGAAGGCCATTCAAGGTTCCAATGAGGCTGCCTGGTTTAGTGTTGATGTGCTTATTTCCGGTGGCTTTGTTGGTGATTCTAATGGCTTTAACTCACAAGACTGTGTTCTTGGTGAGTGCTATAATGACTGTGGCTGGAACTCTATGGTACTTTTTTATGATGCTTTGCAAGAAGAAGAAGATATTCGAGTTCAATGATAGCCCTCCAATCATTCAACAATCATACGACGAAATATCTCCCGAGATCGGTACTCCGACCGCGGATGTTCGACTCGTGATTTAA

mRNA sequence

ATGGATCACCAAGAATTCAGCCACAGCAAACAACAAGCTTTACAGCTCGAATCCGCCACGGTGGTTGCCGCCTCAGCCCCGCCAACCGCCACGGCCTCCACCGCGGCCACCACTCGAAAGAAGCTAACTTTGCTCCCCCTTGTGTTCCTCATTTACTTCCAAGTTGCTGGTGGTCCTTACGGTGAAGAGCCAGCAGTTCAGGCCGCCGGATCGCTATTCGCGATCATCGGCTTCATCGTCTTCCCATTTTTATGGAGTGTCCCGGAGGCATTGATCACGGCAGAGCTCGCCACTACCTTTCCCGGCAATGGCGGTTTCGTGATATGGGCGGAGCGAGCCTTTGGACCCTTTTGTGGCTCCCTCATGGGCACATGGAAGATCTTTAGCGGAATCATGAACATCACCGCCTTCCCGGCGTTCTTCATCGGCTACGTCGAGAAAATAGTCCCGGCCTTGGAATCCGGCTGGCCTCGCCGCATTGCGGTCTTGGTTTCGACGATACTCCTCTCGCTTCTCAACTACGCCGGGTTGACAATCGTCGGCTACGTCGCCGTTGTTTTGGCTTTCGTTTCGCTCTTACCGTTCATCTTAATGACCTTGATCGCCATGCCGAAGATTCGGGCTCGCCGGTGGTTCAGCTCCGGCGAGAAGGGTGTGAAAAAGGACTGGAATTTGTACCTCAATACCCTCTTTTGGAACCTTAACTTCTGGGACAATGTAAGTACGTTGGCTGGTGAAGTTGAGAACCCACAGAAGACTTACCCCATTGCCCTACTTGTTTCTGTTATATTCATCTCTCTCTCTTACATAATTCCGTTGCTTGCTGTCGTCGGCGCCGTCCCGGTCGAACAAAGCGCGTGGGGGTCGGGGTTTCATGCTCAGGCTGCCGAGTTCATCGGCGGGAATTGGCTGAAAATCCTCCTCGATATCGGTGCAGGGTTGTCAGCAATTGGCATGTTCGAAGCGCAGCTGAGCAGCAGTGCGTATCAAATTCTAGGGATGGCGGAGATCGGGATTCTGCCAAAGTTCTTCGCTTCAAGGGCGAAAGGGTTCGAGACGCCATGGGTGGGGATCTTGATATGCACAGCGGTGTCGCTTGGCGTCTCCTACATGAAGTTCTACGACATCGTGGCGTCAGCGAACTTCATTTACAGCTTGGGAATGTTGCTGGAGTTCGCATCATTCATCTGGTTGAGATGGAAGAAGCCGGAGCTGAGAAGGCCATTCAAGGTTCCAATGAGGCTGCCTGGTTTAGTGTTGATGTGCTTATTTCCGGTGGCTTTGTTGGTGATTCTAATGGCTTTAACTCACAAGACTGTGTTCTTGGTGAGTGCTATAATGACTGTGGCTGGAACTCTATGGTACTTTTTTATGATGCTTTGCAAGAAGAAGAAGATATTCGAGTTCAATGATAGCCCTCCAATCATTCAACAATCATACGACGAAATATCTCCCGAGATCGGTACTCCGACCGCGGATGTTCGACTCGTGATTTAA

Coding sequence (CDS)

ATGGATCACCAAGAATTCAGCCACAGCAAACAACAAGCTTTACAGCTCGAATCCGCCACGGTGGTTGCCGCCTCAGCCCCGCCAACCGCCACGGCCTCCACCGCGGCCACCACTCGAAAGAAGCTAACTTTGCTCCCCCTTGTGTTCCTCATTTACTTCCAAGTTGCTGGTGGTCCTTACGGTGAAGAGCCAGCAGTTCAGGCCGCCGGATCGCTATTCGCGATCATCGGCTTCATCGTCTTCCCATTTTTATGGAGTGTCCCGGAGGCATTGATCACGGCAGAGCTCGCCACTACCTTTCCCGGCAATGGCGGTTTCGTGATATGGGCGGAGCGAGCCTTTGGACCCTTTTGTGGCTCCCTCATGGGCACATGGAAGATCTTTAGCGGAATCATGAACATCACCGCCTTCCCGGCGTTCTTCATCGGCTACGTCGAGAAAATAGTCCCGGCCTTGGAATCCGGCTGGCCTCGCCGCATTGCGGTCTTGGTTTCGACGATACTCCTCTCGCTTCTCAACTACGCCGGGTTGACAATCGTCGGCTACGTCGCCGTTGTTTTGGCTTTCGTTTCGCTCTTACCGTTCATCTTAATGACCTTGATCGCCATGCCGAAGATTCGGGCTCGCCGGTGGTTCAGCTCCGGCGAGAAGGGTGTGAAAAAGGACTGGAATTTGTACCTCAATACCCTCTTTTGGAACCTTAACTTCTGGGACAATGTAAGTACGTTGGCTGGTGAAGTTGAGAACCCACAGAAGACTTACCCCATTGCCCTACTTGTTTCTGTTATATTCATCTCTCTCTCTTACATAATTCCGTTGCTTGCTGTCGTCGGCGCCGTCCCGGTCGAACAAAGCGCGTGGGGGTCGGGGTTTCATGCTCAGGCTGCCGAGTTCATCGGCGGGAATTGGCTGAAAATCCTCCTCGATATCGGTGCAGGGTTGTCAGCAATTGGCATGTTCGAAGCGCAGCTGAGCAGCAGTGCGTATCAAATTCTAGGGATGGCGGAGATCGGGATTCTGCCAAAGTTCTTCGCTTCAAGGGCGAAAGGGTTCGAGACGCCATGGGTGGGGATCTTGATATGCACAGCGGTGTCGCTTGGCGTCTCCTACATGAAGTTCTACGACATCGTGGCGTCAGCGAACTTCATTTACAGCTTGGGAATGTTGCTGGAGTTCGCATCATTCATCTGGTTGAGATGGAAGAAGCCGGAGCTGAGAAGGCCATTCAAGGTTCCAATGAGGCTGCCTGGTTTAGTGTTGATGTGCTTATTTCCGGTGGCTTTGTTGGTGATTCTAATGGCTTTAACTCACAAGACTGTGTTCTTGGTGAGTGCTATAATGACTGTGGCTGGAACTCTATGGTACTTTTTTATGATGCTTTGCAAGAAGAAGAAGATATTCGAGTTCAATGATAGCCCTCCAATCATTCAACAATCATACGACGAAATATCTCCCGAGATCGGTACTCCGACCGCGGATGTTCGACTCGTGATTTAA

Protein sequence

MDHQEFSHSKQQALQLESATVVAASAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQSYDEISPEIGTPTADVRLVI
Homology
BLAST of Lcy04g005740 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.8e-165
Identity = 283/443 (63.88%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 29  TATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFLWSVP 88
           TA +S    T KKLTL+PLVFLIYF+VAGGP+GEEPAVQAAG L AI+GF++FPF+WS+P
Sbjct: 17  TAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFIWSIP 76

Query: 89  EALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKI 148
           EALITAEL+T FPGNGGFVIWA RAFG F GS+MG+ K  SG++N+ +FP   + Y++K+
Sbjct: 77  EALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTYLDKL 136

Query: 149 VPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRA 208
            P LESGWPR + +  ST++LS LNY GL IVGY AVVL  VSL PF++M+ +A+PKI+ 
Sbjct: 137 FPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIPKIKP 196

Query: 209 RRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLS 268
            RW S G K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKT+P+ALL++VIF  ++
Sbjct: 197 HRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIFTCVA 256

Query: 269 YIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSA 328
           Y+IPL AV GAV V+QS W +GFHA+AAE I G WLKI ++IGA LS+IG+FEAQLSSSA
Sbjct: 257 YLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQLSSSA 316

Query: 329 YQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGM 388
           YQ+ GMAE+G LPKFF  R+K F TPWVGILI   +SLG+SYM F DI++SANF+Y+LGM
Sbjct: 317 YQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLYTLGM 376

Query: 389 LLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMT 448
            LEFASFIWLR K P+L+RP++VP+++PGLV+MCL P A LV+++    K V+L+  +MT
Sbjct: 377 FLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLICGVMT 436

Query: 449 VAGTLWYFFMMLCKKKKIFEFND 472
           +    WYF +   +K KIFEFN+
Sbjct: 437 IGAIGWYFLINYFRKTKIFEFNE 457

BLAST of Lcy04g005740 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.5e-110
Identity = 213/463 (46.00%), Postives = 314/463 (67.82%), Query Frame = 0

Query: 25  SAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFL 84
           S+PP AT        +K+++LPLVFLI+++V+GGP+G E +V AAG L A++GF++FPF+
Sbjct: 37  SSPPKATDKI-----RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFI 96

Query: 85  WSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGY 144
           WS+PEALITAE+ T +P NGG+V+W   A GPF G   G  K  SG+++   +P  F+ Y
Sbjct: 97  WSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDY 156

Query: 145 VEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMP 204
           ++  VPAL SG PR  ++LV TILL+ LNY GLTIVG+VAV++   S+LPF +M LI++P
Sbjct: 157 LKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIP 216

Query: 205 KIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIF 264
           ++   RW       V  +WNLYLNTLFWNLN+WD++STLAGEVENP  T P AL   VI 
Sbjct: 217 QLEPSRWLVMDLGNV--NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVIL 276

Query: 265 ISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQL 324
           ++ SYI PLLA +GA+P+E+  W  G+ +  A+ +GG WL+  +   A  S +GMF A++
Sbjct: 277 VACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEM 336

Query: 325 SSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIY 384
           SS ++Q+LGMAE G+LP+FFA R++ + TP +GIL   +  + +S++ F +IVA+ N +Y
Sbjct: 337 SSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLLY 396

Query: 385 SLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVS 444
            +GM+LEF +F+ +R K P   RP+K+P+   G +LMC+ P  L+  ++AL+   V  VS
Sbjct: 397 CVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVS 456

Query: 445 AIMTVAGTLWYFFMMLCKKKKIFEF---NDSPPIIQQS--YDE 483
            +M + G L +  +    +K+  +F   +D P + QQ+  Y+E
Sbjct: 457 IVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEE 491

BLAST of Lcy04g005740 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 6.4e-109
Identity = 210/451 (46.56%), Postives = 300/451 (66.52%), Query Frame = 0

Query: 22  VAASAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVF 81
           V    P T+ A T     KK+T+LPLVFLI+++V+GGP+G E +V+AAG L AI+GFIVF
Sbjct: 34  VTTGDPATSPART-VNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVF 93

Query: 82  PFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFF 141
           PF+WS+PEALITAE+ T FP NGG+V+W   A GP+ G   G  K  SG+++   +P  F
Sbjct: 94  PFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILF 153

Query: 142 IGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLI 201
           + Y++  +P L SG PR  A+LV T+ L+ LNY GL+IVG  AV+L   S+LPF++M+ +
Sbjct: 154 LDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFM 213

Query: 202 AMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVS 261
           ++PK++  RW    +K    +W+LYLNTLFWNLN+WD+VSTL GEVENP KT P AL  +
Sbjct: 214 SIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYA 273

Query: 262 VIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFE 321
           ++ +  SYI P+L   GA+ ++Q  W  G+ A   + IGG WL   +   A  S +GMF 
Sbjct: 274 LLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFL 333

Query: 322 AQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASAN 381
           A++SS ++Q+LGMAE G+LP+ FA R++ + TPWVGIL   +  + +S++ F +IVA+ N
Sbjct: 334 AEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSASGVIILSWLSFQEIVAAEN 393

Query: 382 FIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVF 441
            +Y  GM+LEF +F+ LR K P   RPFK+P+ + G VLMC+ P  L+ ++MA T+  V 
Sbjct: 394 LLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVA 453

Query: 442 LVSAIMTVAGTLWYFFMMLCKKKKIFEFNDS 473
           LVS    V G +    +   +KK   +F+ S
Sbjct: 454 LVSLAAIVIGLVLQPCLKQVEKKGWLKFSTS 482

BLAST of Lcy04g005740 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.9e-108
Identity = 208/431 (48.26%), Postives = 294/431 (68.21%), Query Frame = 0

Query: 41  KLTLLPLVFLIYFQVAGGPYGEEPAVQAAGS-LFAIIGFIVFPFLWSVPEALITAELATT 100
           KLTLLPLVFLI+++V+GGP+G E +V++ G  L A++GF++FP +WS+PEAL+TAELAT+
Sbjct: 21  KLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELATS 80

Query: 101 FPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRR 160
           FP NGG+V+W   AFGPF G   G WK FSG+M+   +P  F+ Y++   P L+    R 
Sbjct: 81  FPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAARV 140

Query: 161 IAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGV 220
            A+LV T  L+ LNY GL IVG+ AVVLA  SL PF++M L+A+P IR +RW     +  
Sbjct: 141 PALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ-- 200

Query: 221 KKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGA 280
           K +W  Y NT+FWNLN+WD  STLAGEV+ P KT+P AL  +V+ +  SY+IPL+A  GA
Sbjct: 201 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 260

Query: 281 VPVEQSA-WGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIG 340
           +    S  W  G+ A+    IGG WLK  +   A +S +G+FEA++SS A+Q+LGM+EIG
Sbjct: 261 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 320

Query: 341 ILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWL 400
           +LP FFA R+K + TP + IL      + +S+M F +I+   NF+Y+LGMLLEFA+F+ L
Sbjct: 321 MLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKL 380

Query: 401 RWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFM 460
           R KKP+L RP++VP+   G+ ++CL P  L++++M L     FL+S ++ V G   Y F+
Sbjct: 381 RIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFL 440

Query: 461 MLCKKKKIFEF 470
            L K+K+   F
Sbjct: 441 TLVKEKQWARF 448

BLAST of Lcy04g005740 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 3.2e-108
Identity = 212/452 (46.90%), Postives = 300/452 (66.37%), Query Frame = 0

Query: 37  TTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAEL 96
           ++ + ++++PL+FLI+++V+GGP+G E +V AAG L AIIGF+V P +WS+PEALITAEL
Sbjct: 78  SSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIWSIPEALITAEL 137

Query: 97  ATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGW 156
              FP NGG+V+W   A GP+ G   G  K  SG+++   +P  F+ Y++  VPAL  G 
Sbjct: 138 GAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGA 197

Query: 157 PRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGE 216
           PR  AV+  T +L+LLNY GLT+VG+VA+ L   SLLPF +M LIA+PK+R  RW     
Sbjct: 198 PRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDL 257

Query: 217 KGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAV 276
             V  DWNLYLNTLFWNLN+WD++STLAGEV+NP KT P AL  +VIF+ ++Y+ PLLA 
Sbjct: 258 HNV--DWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAG 317

Query: 277 VGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAE 336
            GAVP+++  W  G+ A  A+ +GG WL   +   A LS +GMF A++SS +YQ+LGMAE
Sbjct: 318 TGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAE 377

Query: 337 IGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFI 396
            G+LP FFA+R++ + TP  GIL   +  L +S M F +IVA+ NF+Y  GMLLEF +FI
Sbjct: 378 RGMLPSFFAARSR-YGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFI 437

Query: 397 WLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYF 456
             R ++P+  RP++VP+   G V M + P AL+ +++AL+   V +VS      G +   
Sbjct: 438 LHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQP 497

Query: 457 FMMLCKKKKIFEFNDSPPIIQQSYDEISPEIG 489
            +   +KK+   F+ +P +         PEIG
Sbjct: 498 ALRFVEKKRWLRFSVNPDL---------PEIG 517

BLAST of Lcy04g005740 vs. ExPASy TrEMBL
Match: A0A5A7U0V1 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00100 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.6e-232
Identity = 423/493 (85.80%), Postives = 451/493 (91.48%), Query Frame = 0

Query: 1   MDHQEFSHSKQQALQLESATVVAASAPPTATASTAA-TTRKKLTLLPLVFLIYFQVAGGP 60
           M+ QEFS+  +QALQ++S    AAS P    A+TAA TTRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MNLQEFSNDNKQALQIQS----AASNPSITDATTAAVTTRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPRRI++ +STILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRRISIFISTILLSLLNYVGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF SLLPFILMTL+AMPKIR RRWFSSGE GVKKDWNLYLNTLFWNLNFWDN
Sbjct: 181 VGYVAIVLAFFSLLPFILMTLMAMPKIRPRRWFSSGENGVKKDWNLYLNTLFWNLNFWDN 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
           VST+AGEVENP KTYP+AL VSVIFISLSYIIPLLAVVGAVPVEQ+AWGSGFHAQAA+FI
Sbjct: 241 VSTMAGEVENPHKTYPVALFVSVIFISLSYIIPLLAVVGAVPVEQTAWGSGFHAQAAQFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
            GNWLKI+LDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFA  AK FETPW+GIL
Sbjct: 301 AGNWLKIILDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFA--AKVFETPWIGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG SYM+FYDIVASANFIYSLGMLLEF SF+WLRWK+PELRRPFKVPM LP LV
Sbjct: 361 ICTVVSLGASYMQFYDIVASANFIYSLGMLLEFTSFLWLRWKQPELRRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           +MCLFP+ALLV+LM LTHKTV +VSAIMT AGTLWYF M LCKKKKIF+FNDSP IIQQS
Sbjct: 421 VMCLFPIALLVVLMILTHKTVLIVSAIMTSAGTLWYFLMKLCKKKKIFKFNDSPQIIQQS 480

Query: 481 YDEISP--EIGTP 491
           Y EIS    +GTP
Sbjct: 481 YIEISTTNHVGTP 487

BLAST of Lcy04g005740 vs. ExPASy TrEMBL
Match: A0A1S3BLI6 (probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491159 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.6e-232
Identity = 423/493 (85.80%), Postives = 451/493 (91.48%), Query Frame = 0

Query: 1   MDHQEFSHSKQQALQLESATVVAASAPPTATASTAA-TTRKKLTLLPLVFLIYFQVAGGP 60
           M+ QEFS+  +QALQ++S    AAS P    A+TAA TTRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MNLQEFSNDNKQALQIQS----AASNPSITDATTAAVTTRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPRRI++ +STILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRRISIFISTILLSLLNYVGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF SLLPFILMTL+AMPKIR RRWFSSGE GVKKDWNLYLNTLFWNLNFWDN
Sbjct: 181 VGYVAIVLAFFSLLPFILMTLMAMPKIRPRRWFSSGENGVKKDWNLYLNTLFWNLNFWDN 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
           VST+AGEVENP KTYP+AL VSVIFISLSYIIPLLAVVGAVPVEQ+AWGSGFHAQAA+FI
Sbjct: 241 VSTMAGEVENPHKTYPVALFVSVIFISLSYIIPLLAVVGAVPVEQTAWGSGFHAQAAQFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
            GNWLKI+LDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFA  AK FETPW+GIL
Sbjct: 301 AGNWLKIILDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFA--AKVFETPWIGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG SYM+FYDIVASANFIYSLGMLLEF SF+WLRWK+PELRRPFKVPM LP LV
Sbjct: 361 ICTVVSLGASYMQFYDIVASANFIYSLGMLLEFTSFLWLRWKQPELRRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           +MCLFP+ALLV+LM LTHKTV +VSAIMT AGTLWYF M LCKKKKIF+FNDSP IIQQS
Sbjct: 421 VMCLFPIALLVVLMILTHKTVLIVSAIMTSAGTLWYFLMKLCKKKKIFKFNDSPQIIQQS 480

Query: 481 YDEISP--EIGTP 491
           Y EIS    +GTP
Sbjct: 481 YIEISTTNHVGTP 487

BLAST of Lcy04g005740 vs. ExPASy TrEMBL
Match: A0A5D3DDX3 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1721G00140 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 7.9e-211
Identity = 396/493 (80.32%), Postives = 423/493 (85.80%), Query Frame = 0

Query: 1   MDHQEFSHSKQQALQLESATVVAASAPPTATASTA-ATTRKKLTLLPLVFLIYFQVAGGP 60
           M+ QEFS+  +QALQ++S    AAS P    A+TA  TTRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MNLQEFSNDNKQALQIQS----AASNPSITDATTATVTTRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPRRI++ +STILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRRISIFISTILLSLLNYVGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF SLLPFILMTL+AMPKIR RRWFSSGE                       
Sbjct: 181 VGYVAIVLAFFSLLPFILMTLMAMPKIRPRRWFSSGE----------------------- 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
                  VENP KTYP+AL VSVIFISLSYIIPLLAVVGAVPVEQ+AWGSGFHAQAA+FI
Sbjct: 241 ------NVENPHKTYPVALFVSVIFISLSYIIPLLAVVGAVPVEQTAWGSGFHAQAAQFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
            GNWLKI+LDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFASRAK FETPW+GIL
Sbjct: 301 AGNWLKIILDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFASRAKVFETPWIGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG SYM+FYDIVASANFIYSLGMLLEF SF+WLRWK+PELRRPFKVPM LP LV
Sbjct: 361 ICTVVSLGASYMQFYDIVASANFIYSLGMLLEFTSFLWLRWKQPELRRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           +MCLFP+ALLV+LM LTHKTV +VSAIMT AGTLWYF M LCKKKKIF+FNDSP IIQQS
Sbjct: 421 VMCLFPIALLVVLMILTHKTVLIVSAIMTSAGTLWYFLMKLCKKKKIFKFNDSPQIIQQS 460

Query: 481 YDEISP--EIGTP 491
           Y EIS    +GTP
Sbjct: 481 YIEISTTNHVGTP 460

BLAST of Lcy04g005740 vs. ExPASy TrEMBL
Match: A0A6J1F0M1 (probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111441072 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.5e-193
Identity = 347/479 (72.44%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 2   DHQEFSHSKQQALQLESATVVAASAPPT-ATASTAATTRKKLTLLPLVFLIYFQVAGGPY 61
           D ++  +    +  L  ++  + + P T ATA+++A  RKKLTL+PL+FLIYF+VAGGPY
Sbjct: 3   DKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPY 62

Query: 62  GEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGS 121
           GEEPAVQAAG L AI+GFIVFPF+WSVPEALITAEL+T FPG+GGFVIWA+RAFGPF GS
Sbjct: 63  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGS 122

Query: 122 LMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIV 181
           LMGTWK+ SG++NI AFP   I Y++KI P LESGWPRRIAV  ST+LL+LLNY GLTIV
Sbjct: 123 LMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLTIV 182

Query: 182 GYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNV 241
           GYVAVVLAF+SLLPFILMT IA+PKI   RW S G+KGVKKDWNLYLNTLFWNLNFWDNV
Sbjct: 183 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNV 242

Query: 242 STLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIG 301
           STLAGEVENPQKT+P+AL VSVIF  +SY+IPLLAV+GAV VEQSAWGSGFHAQAA  I 
Sbjct: 243 STLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 302

Query: 302 GNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILI 361
           G WLKILL+IG+ LS IG+FEAQLSSSAYQILGMAEIGILPKFFASRAK F TPW+GI+I
Sbjct: 303 GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVI 362

Query: 362 CTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVL 421
           CTA+SL VSYM F DIVASANFIYSLGMLLEF++FIWLRWK P ++RPF+VP+RLP L++
Sbjct: 363 CTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIV 422

Query: 422 MCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           MCL P A LV+LM  THK V +VSAIMT AG +W+  M +CK KKI EFN     I++S
Sbjct: 423 MCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEES 481

BLAST of Lcy04g005740 vs. ExPASy TrEMBL
Match: A0A6J1KV06 (probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC111498946 PE=3 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 2.6e-193
Identity = 345/471 (73.25%), Postives = 394/471 (83.65%), Query Frame = 0

Query: 9   SKQQALQLESATVVAASAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQA 68
           +  Q L   S+T            S+AA +RKKLTL+PL+FLIYF+VAGGPYGEEPAVQA
Sbjct: 12  TNSQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVAGGPYGEEPAVQA 71

Query: 69  AGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIF 128
           AG L AI+GFIVFPF+WSVPEALITAEL+T FPG+GGFVIWA+RAFGPF GSLMGTWK+ 
Sbjct: 72  AGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLL 131

Query: 129 SGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLA 188
           SG++NI AFP   I Y++KI P LESGWPRRIAV  ST+LL+LLNY GLTIVGYVAVVLA
Sbjct: 132 SGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLA 191

Query: 189 FVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVE 248
           F+SLLPFILMT IA+PKI   RW S G+KGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVE
Sbjct: 192 FLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVE 251

Query: 249 NPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILL 308
           NPQKT+P+AL VSVIF  +SY+IPLLAV+GAV VEQSAWGSGFHAQAA  I G WLKILL
Sbjct: 252 NPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILL 311

Query: 309 DIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGV 368
           +IG+ LS IG+FEAQLSSSAYQILGMAEIGILPKFFA+RAK F TPW+GI+ICTA+SL V
Sbjct: 312 EIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPWIGIVICTAISLAV 371

Query: 369 SYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVAL 428
           SYM F DIVASANFIYSLGMLLEF++FIWLRWK P ++RPF+VP+RLP L+LMCL P A 
Sbjct: 372 SYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALILMCLIPSAF 431

Query: 429 LVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           LV+LM  THK V +VSA+MT AG +W+  M +CK KKI EFN     I++S
Sbjct: 432 LVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEES 482

BLAST of Lcy04g005740 vs. NCBI nr
Match: XP_008449206.1 (PREDICTED: probable polyamine transporter At3g13620 [Cucumis melo] >KAA0047221.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 815.1 bits (2104), Expect = 3.4e-232
Identity = 423/493 (85.80%), Postives = 451/493 (91.48%), Query Frame = 0

Query: 1   MDHQEFSHSKQQALQLESATVVAASAPPTATASTAA-TTRKKLTLLPLVFLIYFQVAGGP 60
           M+ QEFS+  +QALQ++S    AAS P    A+TAA TTRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MNLQEFSNDNKQALQIQS----AASNPSITDATTAAVTTRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPRRI++ +STILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRRISIFISTILLSLLNYVGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF SLLPFILMTL+AMPKIR RRWFSSGE GVKKDWNLYLNTLFWNLNFWDN
Sbjct: 181 VGYVAIVLAFFSLLPFILMTLMAMPKIRPRRWFSSGENGVKKDWNLYLNTLFWNLNFWDN 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
           VST+AGEVENP KTYP+AL VSVIFISLSYIIPLLAVVGAVPVEQ+AWGSGFHAQAA+FI
Sbjct: 241 VSTMAGEVENPHKTYPVALFVSVIFISLSYIIPLLAVVGAVPVEQTAWGSGFHAQAAQFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
            GNWLKI+LDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFA  AK FETPW+GIL
Sbjct: 301 AGNWLKIILDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFA--AKVFETPWIGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG SYM+FYDIVASANFIYSLGMLLEF SF+WLRWK+PELRRPFKVPM LP LV
Sbjct: 361 ICTVVSLGASYMQFYDIVASANFIYSLGMLLEFTSFLWLRWKQPELRRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           +MCLFP+ALLV+LM LTHKTV +VSAIMT AGTLWYF M LCKKKKIF+FNDSP IIQQS
Sbjct: 421 VMCLFPIALLVVLMILTHKTVLIVSAIMTSAGTLWYFLMKLCKKKKIFKFNDSPQIIQQS 480

Query: 481 YDEISP--EIGTP 491
           Y EIS    +GTP
Sbjct: 481 YIEISTTNHVGTP 487

BLAST of Lcy04g005740 vs. NCBI nr
Match: XP_038882367.1 (probable polyamine transporter At3g13620 [Benincasa hispida])

HSP 1 Score: 786.6 bits (2030), Expect = 1.3e-223
Identity = 408/473 (86.26%), Postives = 429/473 (90.70%), Query Frame = 0

Query: 1   MDHQEF-SHSKQQALQLESATVVAASAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGP 60
           MD QEF S  KQQA Q++ A   A++   T  A+T   TRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MDLQEFRSDDKQQAFQIQPA---ASNPSITGAATTVVATRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLF IIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFVIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPR+IAV VSTILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRQIAVFVSTILLSLLNYFGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF+S LPFILMTLIAMPKIR RRWFSSGE GVK+DWNLYLNTLFWNLNFWDN
Sbjct: 181 VGYVAIVLAFISFLPFILMTLIAMPKIRPRRWFSSGEMGVKRDWNLYLNTLFWNLNFWDN 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
           VSTLAGEVENP KTYP+AL VSVIFISLSYIIPLLAV+GAVPVEQSAWG GFHAQAAEFI
Sbjct: 241 VSTLAGEVENPHKTYPVALFVSVIFISLSYIIPLLAVIGAVPVEQSAWGPGFHAQAAEFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
           GGNWLKILLDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFASRAK FETPWVGIL
Sbjct: 301 GGNWLKILLDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFASRAKVFETPWVGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG S M+FYDIVASANFIYSLGMLLEF SFIWLRWK+PEL RPFKVPM LP LV
Sbjct: 361 ICTGVSLGASCMQFYDIVASANFIYSLGMLLEFTSFIWLRWKQPELWRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDS 473
            MCLFPVALLV+LM LTHK VF VSAIMT+ GTLWY  M LCK+KKIF+FN++
Sbjct: 421 AMCLFPVALLVVLMILTHKIVFFVSAIMTLVGTLWYILMNLCKRKKIFKFNEA 470

BLAST of Lcy04g005740 vs. NCBI nr
Match: TYK21766.1 (putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 743.0 bits (1917), Expect = 1.6e-210
Identity = 396/493 (80.32%), Postives = 423/493 (85.80%), Query Frame = 0

Query: 1   MDHQEFSHSKQQALQLESATVVAASAPPTATASTA-ATTRKKLTLLPLVFLIYFQVAGGP 60
           M+ QEFS+  +QALQ++S    AAS P    A+TA  TTRKKLTLLPLVFLIYFQVAGGP
Sbjct: 1   MNLQEFSNDNKQALQIQS----AASNPSITDATTATVTTRKKLTLLPLVFLIYFQVAGGP 60

Query: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120
           YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG
Sbjct: 61  YGEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCG 120

Query: 121 SLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTI 180
           SLMGTWKIFSGIMNITAFPAFFI YVEKI PALESGWPRRI++ +STILLSLLNY GLTI
Sbjct: 121 SLMGTWKIFSGIMNITAFPAFFISYVEKIFPALESGWPRRISIFISTILLSLLNYVGLTI 180

Query: 181 VGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDN 240
           VGYVA+VLAF SLLPFILMTL+AMPKIR RRWFSSGE                       
Sbjct: 181 VGYVAIVLAFFSLLPFILMTLMAMPKIRPRRWFSSGE----------------------- 240

Query: 241 VSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFI 300
                  VENP KTYP+AL VSVIFISLSYIIPLLAVVGAVPVEQ+AWGSGFHAQAA+FI
Sbjct: 241 ------NVENPHKTYPVALFVSVIFISLSYIIPLLAVVGAVPVEQTAWGSGFHAQAAQFI 300

Query: 301 GGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGIL 360
            GNWLKI+LDIGAGLSAIGM+EAQLSSSAYQILGMAEIGILP+FFASRAK FETPW+GIL
Sbjct: 301 AGNWLKIILDIGAGLSAIGMYEAQLSSSAYQILGMAEIGILPRFFASRAKVFETPWIGIL 360

Query: 361 ICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLV 420
           ICT VSLG SYM+FYDIVASANFIYSLGMLLEF SF+WLRWK+PELRRPFKVPM LP LV
Sbjct: 361 ICTVVSLGASYMQFYDIVASANFIYSLGMLLEFTSFLWLRWKQPELRRPFKVPMELPWLV 420

Query: 421 LMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           +MCLFP+ALLV+LM LTHKTV +VSAIMT AGTLWYF M LCKKKKIF+FNDSP IIQQS
Sbjct: 421 VMCLFPIALLVVLMILTHKTVLIVSAIMTSAGTLWYFLMKLCKKKKIFKFNDSPQIIQQS 460

Query: 481 YDEISP--EIGTP 491
           Y EIS    +GTP
Sbjct: 481 YIEISTTNHVGTP 460

BLAST of Lcy04g005740 vs. NCBI nr
Match: XP_023529220.1 (probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-193
Identity = 349/479 (72.86%), Postives = 403/479 (84.13%), Query Frame = 0

Query: 2   DHQEFSHSKQQALQLESATVVAASAPPT-ATASTAATTRKKLTLLPLVFLIYFQVAGGPY 61
           D ++  +    +  L  ++  + + P T ATA+++A  RKKLTL+PL+FLIYF+VAGGPY
Sbjct: 3   DKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPY 62

Query: 62  GEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGS 121
           GEEPAVQAAG L AI+GFIVFPF+WSVPEALITAEL+T FPG+GGFVIWA+RAFGPF GS
Sbjct: 63  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGS 122

Query: 122 LMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIV 181
           LMGTWK+ SG++NI AFP   I YV+KIVP LESGWPRRIAV  ST+LL+LLNY GLTIV
Sbjct: 123 LMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIV 182

Query: 182 GYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNV 241
           GYVAVVLAF+SLLPFILMT IA+PKI   RW S G+KGVKKDWNLYLNTLFWNLNFWDNV
Sbjct: 183 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNV 242

Query: 242 STLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIG 301
           STLAGEVENPQKT+P+AL VSVIF  +SY+IPLLAV+GAV VEQSAWGSGFHAQAA  I 
Sbjct: 243 STLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 302

Query: 302 GNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILI 361
           G WLKILL+IG+ LS IG+FEAQLSSSAYQILGMAEIGILPKFFASRAK F TPW+GI+I
Sbjct: 303 GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVI 362

Query: 362 CTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVL 421
           CTA+SL VSYM F DIVASANFIYSLGMLLEF++FIWLRWK P ++RPF+VP+RLP L++
Sbjct: 363 CTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIV 422

Query: 422 MCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           MCL P A LV+LM  THK V +VSAIMT AG +W+  M +CK KKI EFN     I++S
Sbjct: 423 MCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEES 481

BLAST of Lcy04g005740 vs. NCBI nr
Match: XP_022933749.1 (probable polyamine transporter At3g13620 [Cucurbita moschata])

HSP 1 Score: 685.6 bits (1768), Expect = 3.1e-193
Identity = 347/479 (72.44%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 2   DHQEFSHSKQQALQLESATVVAASAPPT-ATASTAATTRKKLTLLPLVFLIYFQVAGGPY 61
           D ++  +    +  L  ++  + + P T ATA+++A  RKKLTL+PL+FLIYF+VAGGPY
Sbjct: 3   DKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPY 62

Query: 62  GEEPAVQAAGSLFAIIGFIVFPFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGS 121
           GEEPAVQAAG L AI+GFIVFPF+WSVPEALITAEL+T FPG+GGFVIWA+RAFGPF GS
Sbjct: 63  GEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGS 122

Query: 122 LMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIV 181
           LMGTWK+ SG++NI AFP   I Y++KI P LESGWPRRIAV  ST+LL+LLNY GLTIV
Sbjct: 123 LMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLTIV 182

Query: 182 GYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNV 241
           GYVAVVLAF+SLLPFILMT IA+PKI   RW S G+KGVKKDWNLYLNTLFWNLNFWDNV
Sbjct: 183 GYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNV 242

Query: 242 STLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIG 301
           STLAGEVENPQKT+P+AL VSVIF  +SY+IPLLAV+GAV VEQSAWGSGFHAQAA  I 
Sbjct: 243 STLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIA 302

Query: 302 GNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILI 361
           G WLKILL+IG+ LS IG+FEAQLSSSAYQILGMAEIGILPKFFASRAK F TPW+GI+I
Sbjct: 303 GKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVI 362

Query: 362 CTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVL 421
           CTA+SL VSYM F DIVASANFIYSLGMLLEF++FIWLRWK P ++RPF+VP+RLP L++
Sbjct: 363 CTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIV 422

Query: 422 MCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFMMLCKKKKIFEFNDSPPIIQQS 480
           MCL P A LV+LM  THK V +VSAIMT AG +W+  M +CK KKI EFN     I++S
Sbjct: 423 MCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEES 481

BLAST of Lcy04g005740 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 583.2 bits (1502), Expect = 2.0e-166
Identity = 283/443 (63.88%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 29  TATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFLWSVP 88
           TA +S    T KKLTL+PLVFLIYF+VAGGP+GEEPAVQAAG L AI+GF++FPF+WS+P
Sbjct: 17  TAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFIWSIP 76

Query: 89  EALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKI 148
           EALITAEL+T FPGNGGFVIWA RAFG F GS+MG+ K  SG++N+ +FP   + Y++K+
Sbjct: 77  EALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTYLDKL 136

Query: 149 VPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRA 208
            P LESGWPR + +  ST++LS LNY GL IVGY AVVL  VSL PF++M+ +A+PKI+ 
Sbjct: 137 FPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIPKIKP 196

Query: 209 RRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLS 268
            RW S G K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKT+P+ALL++VIF  ++
Sbjct: 197 HRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIFTCVA 256

Query: 269 YIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSA 328
           Y+IPL AV GAV V+QS W +GFHA+AAE I G WLKI ++IGA LS+IG+FEAQLSSSA
Sbjct: 257 YLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQLSSSA 316

Query: 329 YQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGM 388
           YQ+ GMAE+G LPKFF  R+K F TPWVGILI   +SLG+SYM F DI++SANF+Y+LGM
Sbjct: 317 YQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLYTLGM 376

Query: 389 LLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMT 448
            LEFASFIWLR K P+L+RP++VP+++PGLV+MCL P A LV+++    K V+L+  +MT
Sbjct: 377 FLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLICGVMT 436

Query: 449 VAGTLWYFFMMLCKKKKIFEFND 472
           +    WYF +   +K KIFEFN+
Sbjct: 437 IGAIGWYFLINYFRKTKIFEFNE 457

BLAST of Lcy04g005740 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 213/463 (46.00%), Postives = 314/463 (67.82%), Query Frame = 0

Query: 25  SAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFL 84
           S+PP AT        +K+++LPLVFLI+++V+GGP+G E +V AAG L A++GF++FPF+
Sbjct: 37  SSPPKATDKI-----RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFI 96

Query: 85  WSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGY 144
           WS+PEALITAE+ T +P NGG+V+W   A GPF G   G  K  SG+++   +P  F+ Y
Sbjct: 97  WSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDY 156

Query: 145 VEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMP 204
           ++  VPAL SG PR  ++LV TILL+ LNY GLTIVG+VAV++   S+LPF +M LI++P
Sbjct: 157 LKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIP 216

Query: 205 KIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIF 264
           ++   RW       V  +WNLYLNTLFWNLN+WD++STLAGEVENP  T P AL   VI 
Sbjct: 217 QLEPSRWLVMDLGNV--NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVIL 276

Query: 265 ISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQL 324
           ++ SYI PLLA +GA+P+E+  W  G+ +  A+ +GG WL+  +   A  S +GMF A++
Sbjct: 277 VACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEM 336

Query: 325 SSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIY 384
           SS ++Q+LGMAE G+LP+FFA R++ + TP +GIL   +  + +S++ F +IVA+ N +Y
Sbjct: 337 SSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLLY 396

Query: 385 SLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVS 444
            +GM+LEF +F+ +R K P   RP+K+P+   G +LMC+ P  L+  ++AL+   V  VS
Sbjct: 397 CVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVS 456

Query: 445 AIMTVAGTLWYFFMMLCKKKKIFEF---NDSPPIIQQS--YDE 483
            +M + G L +  +    +K+  +F   +D P + QQ+  Y+E
Sbjct: 457 IVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEE 491

BLAST of Lcy04g005740 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 213/463 (46.00%), Postives = 314/463 (67.82%), Query Frame = 0

Query: 25  SAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVFPFL 84
           S+PP AT        +K+++LPLVFLI+++V+GGP+G E +V AAG L A++GF++FPF+
Sbjct: 21  SSPPKATDKI-----RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFI 80

Query: 85  WSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGY 144
           WS+PEALITAE+ T +P NGG+V+W   A GPF G   G  K  SG+++   +P  F+ Y
Sbjct: 81  WSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDY 140

Query: 145 VEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMP 204
           ++  VPAL SG PR  ++LV TILL+ LNY GLTIVG+VAV++   S+LPF +M LI++P
Sbjct: 141 LKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIP 200

Query: 205 KIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIF 264
           ++   RW       V  +WNLYLNTLFWNLN+WD++STLAGEVENP  T P AL   VI 
Sbjct: 201 QLEPSRWLVMDLGNV--NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVIL 260

Query: 265 ISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQL 324
           ++ SYI PLLA +GA+P+E+  W  G+ +  A+ +GG WL+  +   A  S +GMF A++
Sbjct: 261 VACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEM 320

Query: 325 SSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIY 384
           SS ++Q+LGMAE G+LP+FFA R++ + TP +GIL   +  + +S++ F +IVA+ N +Y
Sbjct: 321 SSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLLY 380

Query: 385 SLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVS 444
            +GM+LEF +F+ +R K P   RP+K+P+   G +LMC+ P  L+  ++AL+   V  VS
Sbjct: 381 CVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVS 440

Query: 445 AIMTVAGTLWYFFMMLCKKKKIFEF---NDSPPIIQQS--YDE 483
            +M + G L +  +    +K+  +F   +D P + QQ+  Y+E
Sbjct: 441 IVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEE 475

BLAST of Lcy04g005740 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.6e-110
Identity = 210/451 (46.56%), Postives = 300/451 (66.52%), Query Frame = 0

Query: 22  VAASAPPTATASTAATTRKKLTLLPLVFLIYFQVAGGPYGEEPAVQAAGSLFAIIGFIVF 81
           V    P T+ A T     KK+T+LPLVFLI+++V+GGP+G E +V+AAG L AI+GFIVF
Sbjct: 34  VTTGDPATSPART-VNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVGFIVF 93

Query: 82  PFLWSVPEALITAELATTFPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFF 141
           PF+WS+PEALITAE+ T FP NGG+V+W   A GP+ G   G  K  SG+++   +P  F
Sbjct: 94  PFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILF 153

Query: 142 IGYVEKIVPALESGWPRRIAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLI 201
           + Y++  +P L SG PR  A+LV T+ L+ LNY GL+IVG  AV+L   S+LPF++M+ +
Sbjct: 154 LDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFM 213

Query: 202 AMPKIRARRWFSSGEKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVS 261
           ++PK++  RW    +K    +W+LYLNTLFWNLN+WD+VSTL GEVENP KT P AL  +
Sbjct: 214 SIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYA 273

Query: 262 VIFISLSYIIPLLAVVGAVPVEQSAWGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFE 321
           ++ +  SYI P+L   GA+ ++Q  W  G+ A   + IGG WL   +   A  S +GMF 
Sbjct: 274 LLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFL 333

Query: 322 AQLSSSAYQILGMAEIGILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASAN 381
           A++SS ++Q+LGMAE G+LP+ FA R++ + TPWVGIL   +  + +S++ F +IVA+ N
Sbjct: 334 AEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSASGVIILSWLSFQEIVAAEN 393

Query: 382 FIYSLGMLLEFASFIWLRWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVF 441
            +Y  GM+LEF +F+ LR K P   RPFK+P+ + G VLMC+ P  L+ ++MA T+  V 
Sbjct: 394 LLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVA 453

Query: 442 LVSAIMTVAGTLWYFFMMLCKKKKIFEFNDS 473
           LVS    V G +    +   +KK   +F+ S
Sbjct: 454 LVSLAAIVIGLVLQPCLKQVEKKGWLKFSTS 482

BLAST of Lcy04g005740 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-109
Identity = 208/431 (48.26%), Postives = 294/431 (68.21%), Query Frame = 0

Query: 41  KLTLLPLVFLIYFQVAGGPYGEEPAVQAAGS-LFAIIGFIVFPFLWSVPEALITAELATT 100
           KLTLLPLVFLI+++V+GGP+G E +V++ G  L A++GF++FP +WS+PEAL+TAELAT+
Sbjct: 21  KLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELATS 80

Query: 101 FPGNGGFVIWAERAFGPFCGSLMGTWKIFSGIMNITAFPAFFIGYVEKIVPALESGWPRR 160
           FP NGG+V+W   AFGPF G   G WK FSG+M+   +P  F+ Y++   P L+    R 
Sbjct: 81  FPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAARV 140

Query: 161 IAVLVSTILLSLLNYAGLTIVGYVAVVLAFVSLLPFILMTLIAMPKIRARRWFSSGEKGV 220
            A+LV T  L+ LNY GL IVG+ AVVLA  SL PF++M L+A+P IR +RW     +  
Sbjct: 141 PALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ-- 200

Query: 221 KKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTYPIALLVSVIFISLSYIIPLLAVVGA 280
           K +W  Y NT+FWNLN+WD  STLAGEV+ P KT+P AL  +V+ +  SY+IPL+A  GA
Sbjct: 201 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 260

Query: 281 VPVEQSA-WGSGFHAQAAEFIGGNWLKILLDIGAGLSAIGMFEAQLSSSAYQILGMAEIG 340
           +    S  W  G+ A+    IGG WLK  +   A +S +G+FEA++SS A+Q+LGM+EIG
Sbjct: 261 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 320

Query: 341 ILPKFFASRAKGFETPWVGILICTAVSLGVSYMKFYDIVASANFIYSLGMLLEFASFIWL 400
           +LP FFA R+K + TP + IL      + +S+M F +I+   NF+Y+LGMLLEFA+F+ L
Sbjct: 321 MLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKL 380

Query: 401 RWKKPELRRPFKVPMRLPGLVLMCLFPVALLVILMALTHKTVFLVSAIMTVAGTLWYFFM 460
           R KKP+L RP++VP+   G+ ++CL P  L++++M L     FL+S ++ V G   Y F+
Sbjct: 381 RIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFL 440

Query: 461 MLCKKKKIFEF 470
            L K+K+   F
Sbjct: 441 TLVKEKQWARF 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN72.8e-16563.88Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S51.5e-11046.00Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9FFL16.4e-10946.56Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9LH391.9e-10848.26Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
A2X8M83.2e-10846.90Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A5A7U0V11.6e-23285.80Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3BLI61.6e-23285.80probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491159... [more]
A0A5D3DDX37.9e-21180.32Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1F0M11.5e-19372.44probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KV062.6e-19373.25probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
Match NameE-valueIdentityDescription
XP_008449206.13.4e-23285.80PREDICTED: probable polyamine transporter At3g13620 [Cucumis melo] >KAA0047221.1... [more]
XP_038882367.11.3e-22386.26probable polyamine transporter At3g13620 [Benincasa hispida][more]
TYK21766.11.6e-21080.32putative polyamine transporter [Cucumis melo var. makuwa][more]
XP_023529220.11.1e-19372.86probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo][more]
XP_022933749.13.1e-19372.44probable polyamine transporter At3g13620 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G13620.12.0e-16663.88Amino acid permease family protein [more]
AT1G31830.11.1e-11146.00Amino acid permease family protein [more]
AT1G31830.21.1e-11146.00Amino acid permease family protein [more]
AT5G05630.14.6e-11046.56Amino acid permease family protein [more]
AT3G19553.11.3e-10948.26Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 37..474
e-value: 1.3E-58
score: 200.7
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 16..473
e-value: 1.9E-63
score: 212.1
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 23..472
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 65..447
e-value: 1.6E-33
score: 116.3
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 23..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy04g005740.1Lcy04g005740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity