Lcy04g003360 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g003360
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Pectin lyase-like superfamily protein
Location	Chr04: 24903063 .. 24905549 (-)
RNA-Seq Expression	Lcy04g003360
Synteny	Lcy04g003360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATTTGGAACTCTCATTCCAATTTCCAAGTTAGCAATTTAAATAAGGAGAAAATAAGCAAAGTCGAAGGTATATACATCGAGGATGGGATTTAGAATTGAAGGTGTTAATATCTAATATGCAGAAACAATTTTGGATCTTAGGCACTTAATAACACTTCAATTTGATCATAAAAACATGCTGTTCATATGAAGAAAAACAGGAGCAATGAGTACCAACCTCTTTATTCTTCAAATTGGATAAATTGGTTGCTTGTTTTTCTTCACCAAATTGTCACGAACCACCACTTGATCTTCCTTCCTATCCTCAAGCCTTTGGAACGGATTGTGGGATCCTCTAGTGATAGAATTTGGAAGGGAAAGAGCTTGAAAACCAAGCTTCAATGGAGGTTTTGAGTTCTTGAGTTTTGGAGAGATTTTGGCTGCATTTCTCTATGGATTCAAGCATTGTTTCTTCAGCAAATTCACCTCCATTTATAGAGGGACTCAAGCTGAGAAGATGCACCAAGATTTGACACCAAATTACCCAAAGGTTAGTGGGTAATTTGGTGGCTTAATGAGGGGGAGTGCATGGGTTTAGTCTCCATGCACTCCACCTCCTAAAATTCAAATTTTATTTAATTCCATAATTAATAATATGAAAATTAAAATCCAATTTTGAATTTTGAATTTTAAATTAATTCAAAATTAATTTAAAATAATTATTAATTAAATAATTAAATATATAATTAATTTAATATCTAATATTAAATTAATATTTTACTACCAATCCCGAACATTTCCGAAATCTTATTTTTCAACAATGAATCCCTATTCATGTGCTTGATATTTAAATCATATTTAAATATATTAAATCTCTCATAACTTGGTTTATCAAAAACTCGACGTGTCGAAAATATCAAATATGACCAACCATTTCCCTAAACCGAATTCGAACTTATTCTAATTCCTTCCATCATATTGTTCCAAAGGTTATTCTCAAGCTAGCAAGGGGACCTAATGGACCTACAGATCATGGGTTCCAACGGTTCGAGGTTTAACCGGCTAAACTCTTTTAACCCAGTTAACCAACATCCGTTAACTACTGGGTCACTCCACTATATAGCCTAGTAGTTGAACTCCCCTCACTGTAGATATATTTCCGTCCACTTGATATAAACATGATTAGTAAGTCAATCCTTCACAGGTCGTTTGTAACTATAGCTAGGTCAAAGGTCTGTTTTACCCCTATAGCACATCTTGATTCCTTAAGTCCACTGGTCCTGTAATGAACAGTTTGTTCATGGTCCAACCATTAAACAGAGTCCCTCTCAAGCCAATGAGAGGTTGGAGCCCATTGTTCAAGCTCCAGAGTCAGCACTTAAGGGAACAACCTTTCTACTATCCCTGATTTTGGTAGGAGTGACTTCCGTCTTGCAATACTATGTCCCCAGCTATTCATCTAGTCTTATCCCCAAAATGGTAGGCTTATTGAGCGACATTGTTGGGTCTCTCTCACCCATGCAAATCAAAGGATAATCCCAAACAAACAGGAGTTCATAGTAACCTTAGGATTAAGATCGAGTTACCTAGGTCATCGCATGAAATAGTCAGTCTTAACAGTAAACGGCGTTAGAAAGTAAGAGTGACTTATTTCTTCATCCGGTCTTGTGTAAACATCTTTTACACAGGTTGCCCCTACTTGCATGTCTCTACATGAACGATTCAGGATCACGTCGTTTGCCTCGTTCGTAAAGTAGGCCACATCCATAGTGTTACCAGGATAGGTACCCAACCTTATCCCTATACTATAGACCATGATTGTGTGTCTATAGAAGATAGTAACAAACAAGTACCTATTACTGACGTAACTTGCGGACCAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACCAAGGAAAAAGACGTGGAAGGAGTTTTTGTGAAAAAATGCAAAATAATGAGTACTACAAATGGTGTCAGGATCAAAATATGGTCAGATTCTTCTGTCGCATTCTCAGCCTCTGACGTGCATTTTGAAGATATTGAAATGGTTAATGTCAGCAATCCTGTCATTATAGACCAAGAATATTGTCTATGAAATCAATACAATCAAAAGGTATATGTGGAATTATGTCTCATTTATTTTCAACTTATTATTAACTTTAAAAAGTATTATAAAAAATGCAAAAGTTGAGTTATTCGTATGTGTTAATATATATATATTGGTATCTTTAGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATTAGAGGCACTTCTGCTACTCCAGAAGCAGTCAAACTTATGTGTAGCAAAAGTATACCATGTGAAGGTCTAGAAGTAGTTGATATTGACCTTACTTACAGTGGAACCCAAGGACCGATTACGTCCCAGTGTTCGAATGTGAAACCCACCATTACTGGAAAACAAAACCCTCGCATATGTACTGAACTTGCCCCTATTGATTCTCCATCGACTGACTAA

mRNA sequence

ATGGATATTTGGAACTCTCATTCCAATTTCCAAGTTAGCAATTTAAATAAGGAGAAAATAAGCAAAGTCGAAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACCAAGGAAAAAGACGTGGAAGGAGTTTTTGTGAAAAAATGCAAAATAATGAGTACTACAAATGGTGTCAGGATCAAAATATGGTCAGATTCTTCTGTCGCATTCTCAGCCTCTGACGTGCATTTTGAAGATATTGAAATGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATTAGAGGCACTTCTGCTACTCCAGAAGCAGTCAAACTTATGTGTAGCAAAAGTATACCATGTGAAGGTCTAGAAGTAGTTGATATTGACCTTACTTACAGTGGAACCCAAGGACCGATTACGTCCCAGTGTTCGAATGTGAAACCCACCATTACTGGAAAACAAAACCCTCGCATATGTACTGAACTTGCCCCTATTGATTCTCCATCGACTGACTAA

Coding sequence (CDS)

ATGGATATTTGGAACTCTCATTCCAATTTCCAAGTTAGCAATTTAAATAAGGAGAAAATAAGCAAAGTCGAAGGACATGGCATTAGCATAGGAAGTTTGGGCAAGTACACCAAGGAAAAAGACGTGGAAGGAGTTTTTGTGAAAAAATGCAAAATAATGAGTACTACAAATGGTGTCAGGATCAAAATATGGTCAGATTCTTCTGTCGCATTCTCAGCCTCTGACGTGCATTTTGAAGATATTGAAATGGTTGCATCAAAAATTAAGATCAGCAAAGTTAGCTTCAAGAATATTAGAGGCACTTCTGCTACTCCAGAAGCAGTCAAACTTATGTGTAGCAAAAGTATACCATGTGAAGGTCTAGAAGTAGTTGATATTGACCTTACTTACAGTGGAACCCAAGGACCGATTACGTCCCAGTGTTCGAATGTGAAACCCACCATTACTGGAAAACAAAACCCTCGCATATGTACTGAACTTGCCCCTATTGATTCTCCATCGACTGACTAA

Protein sequence

MDIWNSHSNFQVSNLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEMVASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD

Homology

[Close]

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.1e-39
Identity = 86/167 (51.50%), Postives = 106/167 (63.47%), Query Frame = 0


Query: 14  NLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSA 73
           N+N   I+   GHGIS+GSLG+Y  E+ V G++VK C I  + NGVRIK W  S     A
Sbjct: 190 NINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKSEPG-EA 249

Query: 74  SDVHFEDIEM----------------------VASKIKISKVSFKNIRGTSATPEAVKLM 133
           S++HF+DI M                      V S +K+SK+SFKNI+GTS T EAVKL+
Sbjct: 250 SEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAVKLV 309

Query: 134 CSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICT 159
           CSKS PC G+E+ DIDLTYSG  GP TS C N+KPTI GKQ P IC+
Sbjct: 310 CSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICS 355

[Close]

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.7e-35
Identity = 81/163 (49.69%), Postives = 101/163 (61.96%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDI--- 84
           GHGIS+GSLG    EK V GVFV+ C   +T NGVRIK W  S      +DVHFEDI   
Sbjct: 235 GHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGV-VNDVHFEDIIVQ 294

Query: 85  -------------------EMVASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                              + + S++KISKVSF+NI+GTS T +AV L+ SK +PCEG+E
Sbjct: 295 NVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIE 354

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDS 166
           V DID+TYSG +GP  S C N+KP++ GKQNP +CT  A   S
Sbjct: 355 VGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVCTASAASSS 396

[Close]

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot
Match: W8P8Q3 (Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 PE=1 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 2.3e-34
Identity = 79/166 (47.59%), Postives = 102/166 (61.45%), Query Frame = 0


Query: 14  NLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSA 73
           +++ E I+   GHGISIGS+GK+  E    G+FVK C    T NGVRIK W +S  A SA
Sbjct: 230 DVHVEGITCGPGHGISIGSMGKFANEAPNTGIFVKNCSFTDTDNGVRIKSWMNSFEA-SA 289

Query: 74  SDVHFEDI----------------------EMVASKIKISKVSFKNIRGTSATPEAVKLM 133
           SD+HFEDI                      E   SK+K+SK+SFKN+ G + + E VKL+
Sbjct: 290 SDLHFEDITVTNVLNPVIIDQEYCPYNHCKEKTPSKVKLSKISFKNVHGAAKSAEVVKLL 349

Query: 134 CSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRIC 158
           CS ++PC+G+E+ DIDLT+ G  G   SQC NVKP +TGKQNP  C
Sbjct: 350 CSSAVPCDGVELADIDLTFPG--GAAVSQCKNVKPIVTGKQNPVAC 392

[Close]

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 5.7e-33
Identity = 79/170 (46.47%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 14  NLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSA 73
           NL  E +    GHGISIGSLG+Y  E+ V+GV V+KC I +T NGVRIK W  S     A
Sbjct: 272 NLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI-A 331

Query: 74  SDVHFEDIEM----------------------VASKIKISKVSFKNIRGTSATPEAVKLM 133
           S++ FEDI M                      V S++K+S V+ K I+GTSAT  AVKLM
Sbjct: 332 SNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLM 391

Query: 134 CSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELA 162
           CSK +PC  + + DI+L ++G +GP  S CSN+KP ++GK  P  CTE+A
Sbjct: 392 CSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAACTEVA 440

[Close]

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 5.7e-33
Identity = 69/148 (46.62%), Postives = 98/148 (66.22%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEMV 84
           GHG+S+GSLGK+T E++VEG+ VK C + +T NGVRIK W D+    + SD+HFEDI M 
Sbjct: 241 GHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMT 300

Query: 85  ----------------------ASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                  SKIK+SK+SFKN++GTS T E V L+CS ++PC+G+E
Sbjct: 301 NVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVE 360

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITG 151
           + ++DL ++G   P T++C+NVKP +TG
Sbjct: 361 LNNVDLKFNG--APTTAKCTNVKPLVTG 386

[Close]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL
Match: A0A6J1DZB6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024445 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.2e-56
Identity = 114/167 (68.26%), Postives = 133/167 (79.64%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGI+IGSLGKYT+E+ VEGV +KKCK++ TTNGVRIK W DS+ +F ASD+HFEDIEM 
Sbjct: 238 GHGIAIGSLGKYTREQGVEGVTIKKCKLIDTTNGVRIKTWPDSAGSFGASDMHFEDIEMV 297

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                V SKIK+SKVSFKNI+GTSATP AVKL+CSKSIPCEG+E
Sbjct: 298 NVSNPVIIDQEYCPWNQCNRKVPSKIKVSKVSFKNIKGTSATPVAVKLVCSKSIPCEGVE 357

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           V DIDLTYSG+QGPITSQC+NVKPTI+GKQNP+IC E+AP+D PSTD
Sbjct: 358 VADIDLTYSGSQGPITSQCANVKPTISGKQNPKICAEVAPLDGPSTD 404

[Close]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL
Match: A0A6J1DCB0 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019177 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.5e-54
Identity = 113/167 (67.66%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKY+KEK+V GV VKKCK+++T+NGVRIK W D++V F A+D+HFEDIEM 
Sbjct: 241 GHGISIGSLGKYSKEKEVVGVTVKKCKLINTSNGVRIKTWPDAAVTFPATDMHFEDIEMT 300

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                V S IKISKVSFKNIRGTSATP AVKL+CSKS+PCE +E
Sbjct: 301 NVSNPVIIDQEYCPWNQCNRKVPSSIKISKVSFKNIRGTSATPIAVKLVCSKSVPCEDVE 360

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           V DIDLTYSG+QGPI SQC+NVKPTITGKQNP IC E+AP D+PSTD
Sbjct: 361 VADIDLTYSGSQGPIKSQCANVKPTITGKQNPPICAEVAPADAPSTD 407

[Close]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL
Match: A0A6J1C2U8 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007858 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.0e-53
Identity = 114/167 (68.26%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKY+KEKDV GVFVKKCK+ STTNGVRIK W DS+ AF ASD+HFEDIEM 
Sbjct: 246 GHGISIGSLGKYSKEKDVIGVFVKKCKLTSTTNGVRIKTWPDSAGAFIASDMHFEDIEMV 305

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                V SKIKISKVSFKNIRGTSATP AVKL+CS S PCEG+E
Sbjct: 306 NVSNPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPVAVKLVCSSSNPCEGVE 365

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           V DI+LTY+G QGPI S+C+NVKPTITG+QNP+IC ++AP ++PSTD
Sbjct: 366 VADINLTYNGKQGPIKSECANVKPTITGQQNPKICAKVAPPNAPSTD 412

[Close]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL
Match: A0A6J1DJH0 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111021092 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.3e-53
Identity = 110/167 (65.87%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKYTKEK+V GV VKKCK+++T+NGVRIK W D++V+F A+D+HFEDIEM 
Sbjct: 221 GHGISIGSLGKYTKEKEVVGVTVKKCKLINTSNGVRIKTWPDAAVSFPATDMHFEDIEMT 280

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                V S IKISKVSFKNIRGTSATP AVK++CSKS+PCEG+E
Sbjct: 281 NVSFPIIIDQEYCPWNQCNRKVPSSIKISKVSFKNIRGTSATPIAVKIVCSKSVPCEGVE 340

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           + DIDLTY+G+QGPI SQC+NVKP ITGKQNP IC E+AP D+PS D
Sbjct: 341 IADIDLTYNGSQGPIKSQCANVKPVITGKQNPPICAEVAPADAPSID 387

[Close]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL
Match: A0A5D3D708 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00680 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 119/184 (64.67%), Postives = 134/184 (72.83%), Query Frame = 0


Query: 8   SNFQVSNLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDS 67
           SN QV+  N   ++   GHGISIGSLGKYTKEKDV GV VK CKI +TTNGVRIK W DS
Sbjct: 220 SNKQVTITN---VTCGPGHGISIGSLGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDS 279

Query: 68  SVAFSASDVHFEDIEM----------------------VASKIKISKVSFKNIRGTSATP 127
           +VAF ASD+HFEDIEM                      V SKIKIS VSFKNIRGTSATP
Sbjct: 280 AVAFMASDMHFEDIEMENVSNPIIIDQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATP 339

Query: 128 EAVKLMCSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDS 170
            AVKL+CSKSIPCEG+EV DI+LTYSG +GPI S+C+NV PTITG QNP+IC + A I +
Sbjct: 340 VAVKLVCSKSIPCEGVEVADINLTYSGNRGPIVSECANVMPTITGVQNPQICAKPATIGA 399

[Close]

BLAST of Lcy04g003360 vs. NCBI nr
Match: XP_022157826.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 228.4 bits (581), Expect = 4.6e-56
Identity = 114/167 (68.26%), Postives = 133/167 (79.64%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGI+IGSLGKYT+E+ VEGV +KKCK++ TTNGVRIK W DS+ +F ASD+HFEDIEM 
Sbjct: 238 GHGIAIGSLGKYTREQGVEGVTIKKCKLIDTTNGVRIKTWPDSAGSFGASDMHFEDIEMV 297

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                V SKIK+SKVSFKNI+GTSATP AVKL+CSKSIPCEG+E
Sbjct: 298 NVSNPVIIDQEYCPWNQCNRKVPSKIKVSKVSFKNIKGTSATPVAVKLVCSKSIPCEGVE 357

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           V DIDLTYSG+QGPITSQC+NVKPTI+GKQNP+IC E+AP+D PSTD
Sbjct: 358 VADIDLTYSGSQGPITSQCANVKPTISGKQNPKICAEVAPLDGPSTD 404

[Close]

BLAST of Lcy04g003360 vs. NCBI nr
Match: XP_011657634.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 5.6e-54
Identity = 115/167 (68.86%), Postives = 127/167 (76.05%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKYTKEK+V GV VKKCK+  TTNGVRIK W DS+VAF A+D+HFEDIEM 
Sbjct: 253 GHGISIGSLGKYTKEKEVVGVTVKKCKLTGTTNGVRIKTWPDSAVAFPATDMHFEDIEMD 312

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                VASKIKIS VSFKNIRGTSAT  AVK++CSKSIPCEG+E
Sbjct: 313 NVSNPIIIDQEYCPWNQCNRKVASKIKISNVSFKNIRGTSATAIAVKIVCSKSIPCEGVE 372

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           + DIDLTYSG +GPI SQC+NVKP ITGKQNP IC E+AP D PSTD
Sbjct: 373 IADIDLTYSGPEGPIKSQCANVKPVITGKQNPPICGEVAPADGPSTD 419

[Close]

BLAST of Lcy04g003360 vs. NCBI nr
Match: XP_031742832.1 (exopolygalacturonase-like [Cucumis sativus] >XP_031742833.1 exopolygalacturonase-like [Cucumis sativus] >XP_031742834.1 exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 5.6e-54
Identity = 115/167 (68.86%), Postives = 127/167 (76.05%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKYTKEK+V GV VKKCK+  TTNGVRIK W DS+VAF A+D+HFEDIEM 
Sbjct: 253 GHGISIGSLGKYTKEKEVVGVTVKKCKLTGTTNGVRIKTWPDSAVAFPATDMHFEDIEMD 312

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                VASKIKIS VSFKNIRGTSAT  AVK++CSKSIPCEG+E
Sbjct: 313 NVSNPIIIDQEYCPWNQCNRKVASKIKISNVSFKNIRGTSATAIAVKIVCSKSIPCEGVE 372

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           + DIDLTYSG +GPI SQC+NVKP ITGKQNP IC E+AP D PSTD
Sbjct: 373 IADIDLTYSGPEGPIKSQCANVKPVITGKQNPPICGEVAPADGPSTD 419

[Close]

BLAST of Lcy04g003360 vs. NCBI nr
Match: XP_031742831.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 5.6e-54
Identity = 115/167 (68.86%), Postives = 127/167 (76.05%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKYTKEK+V GV VKKCK+  TTNGVRIK W DS+VAF A+D+HFEDIEM 
Sbjct: 284 GHGISIGSLGKYTKEKEVVGVTVKKCKLTGTTNGVRIKTWPDSAVAFPATDMHFEDIEMD 343

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                VASKIKIS VSFKNIRGTSAT  AVK++CSKSIPCEG+E
Sbjct: 344 NVSNPIIIDQEYCPWNQCNRKVASKIKISNVSFKNIRGTSATAIAVKIVCSKSIPCEGVE 403

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           + DIDLTYSG +GPI SQC+NVKP ITGKQNP IC E+AP D PSTD
Sbjct: 404 IADIDLTYSGPEGPIKSQCANVKPVITGKQNPPICGEVAPADGPSTD 450

[Close]

BLAST of Lcy04g003360 vs. NCBI nr
Match: KAE8647377.1 (hypothetical protein Csa_004085 [Cucumis sativus])

HSP 1 Score: 221.5 bits (563), Expect = 5.6e-54
Identity = 115/167 (68.86%), Postives = 127/167 (76.05%), Query Frame = 0


Query: 25  GHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSASDVHFEDIEM- 84
           GHGISIGSLGKYTKEK+V GV VKKCK+  TTNGVRIK W DS+VAF A+D+HFEDIEM 
Sbjct: 284 GHGISIGSLGKYTKEKEVVGVTVKKCKLTGTTNGVRIKTWPDSAVAFPATDMHFEDIEMD 343

Query: 85  ---------------------VASKIKISKVSFKNIRGTSATPEAVKLMCSKSIPCEGLE 144
                                VASKIKIS VSFKNIRGTSAT  AVK++CSKSIPCEG+E
Sbjct: 344 NVSNPIIIDQEYCPWNQCNRKVASKIKISNVSFKNIRGTSATAIAVKIVCSKSIPCEGVE 403

Query: 145 VVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSPSTD 170
           + DIDLTYSG +GPI SQC+NVKP ITGKQNP IC E+AP D PSTD
Sbjct: 404 IADIDLTYSGPEGPIKSQCANVKPVITGKQNPPICGEVAPADGPSTD 450

[Close]

BLAST of Lcy04g003360 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-37
Identity = 76/169 (44.97%), Postives = 104/169 (61.54%), Query Frame = 0


Query: 12  VSNLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAF 71
           + NL+ EK++   GHGIS+GSLG+Y  E+DV G+ V  C +  T NG+RIK W  ++ + 
Sbjct: 219 MKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACST 278

Query: 72  SASDVHFEDI----------------------EMVASKIKISKVSFKNIRGTSATPEAVK 131
           +ASD+HFEDI                      +  AS IK+  +SFKNIRGTS   +AVK
Sbjct: 279 TASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVK 338

Query: 132 LMCSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICT 159
           L+CSK  PC+ +E+ DID+ Y+G  GP T  CSNV P I G Q+P+ C+
Sbjct: 339 LLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPKACS 387

[Close]

BLAST of Lcy04g003360 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 2.8e-35
Identity = 81/170 (47.65%), Postives = 104/170 (61.18%), Query Frame = 0


Query: 14  NLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSA 73
           NL  E +    GHGISIGSLG+Y  E+ V+GV V+KC I +T NGVRIK W  S     A
Sbjct: 273 NLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI-A 332

Query: 74  SDVHFEDIEM----------------------VASKIKISKVSFKNIRGTSATPEAVKLM 133
           S++ FEDI M                      V SK+K+S V+ KNI+GTSAT  AVKLM
Sbjct: 333 SNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLM 392

Query: 134 CSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELA 162
           CSK +PC  + + DI+L ++G +GP  S CSN+KP ++GK  P  CTE+A
Sbjct: 393 CSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAACTEVA 441

[Close]

BLAST of Lcy04g003360 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 4.0e-34
Identity = 79/170 (46.47%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 14  NLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAFSA 73
           NL  E +    GHGISIGSLG+Y  E+ V+GV V+KC I +T NGVRIK W  S     A
Sbjct: 272 NLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI-A 331

Query: 74  SDVHFEDIEM----------------------VASKIKISKVSFKNIRGTSATPEAVKLM 133
           S++ FEDI M                      V S++K+S V+ K I+GTSAT  AVKLM
Sbjct: 332 SNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLM 391

Query: 134 CSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELA 162
           CSK +PC  + + DI+L ++G +GP  S CSN+KP ++GK  P  CTE+A
Sbjct: 392 CSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAACTEVA 440

[Close]

BLAST of Lcy04g003360 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 4.0e-34
Identity = 72/177 (40.68%), Postives = 106/177 (59.89%), Query Frame = 0


Query: 12  VSNLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAF 71
           + NL  E+++   GHGISIGSLG+Y+ E++V G+ +  C +  T NG+RIK W  ++   
Sbjct: 219 MKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTT 278

Query: 72  SASDVHFEDI----------------------EMVASKIKISKVSFKNIRGTSATPEAVK 131
           +ASD+HFE+I                      +   S IK++ +SFK IRGTS   +AVK
Sbjct: 279 TASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVK 338

Query: 132 LMCSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICTELAPIDSP 167
           L+CSK  PC+ +EV D+++ Y+G  GP T QCSNV P + G Q P+ C+  +P+  P
Sbjct: 339 LLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACS--SPVTKP 393

[Close]

BLAST of Lcy04g003360 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 1.5e-33
Identity = 72/169 (42.60%), Postives = 99/169 (58.58%), Query Frame = 0


Query: 12  VSNLNKEKISKVEGHGISIGSLGKYTKEKDVEGVFVKKCKIMSTTNGVRIKIWSDSSVAF 71
           + NL+ E +    GHGIS+GSLG+Y  E+DV G+ V  C +  T NG+RIK W  ++ A 
Sbjct: 220 MKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACAT 279

Query: 72  SASDVHFEDI----------------------EMVASKIKISKVSFKNIRGTSATPEAVK 131
           +AS +HFE+I                      +   S IK+  +SFKNIRGTS   +AVK
Sbjct: 280 TASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVK 339

Query: 132 LMCSKSIPCEGLEVVDIDLTYSGTQGPITSQCSNVKPTITGKQNPRICT 159
           L+CSK+ PC  +E+ +I+L Y G  GP T  CSNV P + G QNP+ C+
Sbjct: 340 LLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPKACS 388

The following BLAST results are available for this feature:

BLAST of Lcy04g003360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	3.1e-39	51.50	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q05967	2.7e-35	49.69	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
W8P8Q3	2.3e-34	47.59	Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 P...	[more]
P49063	5.7e-33	46.47	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]
Q40312	5.7e-33	46.62	Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1	[more]

BLAST of Lcy04g003360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZB6	2.2e-56	68.26	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024445 PE=3 SV...	[more]
A0A6J1DCB0	3.5e-54	67.66	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019177 PE=3 SV...	[more]
A0A6J1C2U8	1.0e-53	68.26	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007858 PE=3 SV...	[more]
A0A6J1DJH0	1.3e-53	65.87	polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111021092 PE=3 SV=1	[more]
A0A5D3D708	1.1e-52	64.67	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Lcy04g003360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157826.1	4.6e-56	68.26	exopolygalacturonase-like [Momordica charantia]	[more]
XP_011657634.1	5.6e-54	68.86	exopolygalacturonase [Cucumis sativus]	[more]
XP_031742832.1	5.6e-54	68.86	exopolygalacturonase-like [Cucumis sativus] >XP_031742833.1 exopolygalacturonase...	[more]
XP_031742831.1	5.6e-54	68.86	exopolygalacturonase-like [Cucumis sativus]	[more]
KAE8647377.1	5.6e-54	68.86	hypothetical protein Csa_004085 [Cucumis sativus]	[more]

BLAST of Lcy04g003360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07820.1	3.9e-37	44.97	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	2.8e-35	47.65	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	4.0e-34	46.47	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	4.0e-34	40.68	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	1.5e-33	42.60	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 85..146 e-value: 1.8E-11 score: 43.7 coord: 14..84 e-value: 4.7E-18 score: 65.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 3..165 e-value: 1.2E-30 score: 108.8
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 86..161
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 14..83
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 14..83
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 86..161
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 3..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g003360.1	Lcy04g003360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity