Lcy04g001500 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy04g001500
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	cysteine proteinase inhibitor A-like
Location	Chr04: 7331061 .. 7332885 (-)
RNA-Seq Expression	Lcy04g001500
Synteny	Lcy04g001500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGTGGCCGGAGGATTTCCTCTCACACCTCCAACACCAATTGAGGATATCAACAACCCATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGGAAAGATCTGAAGTTCAAGCGCGTGGTGAATGGTCTGATACAGACTGGTATTCTGGCCGGGTTTCGTTCACTTAGAATATATGAAATTGTGGTGGAGGCTCAAACCACCAATGAAATTCTTTGGACATATGTGGCTAAGGGTGATGAGCAAGACAGTATGATTCACTACAAAAAAGTTGGCCTATTTCGACCATGTTTTATCGAAGGTTATAAAAAACCTTTGAAAAAGATATGCATTCATCAAAGGTATTTAAATAACCTTTGAAAAATATCAACTTTTATCGAAGGTTCTTCAAAACCTTTGAAGAAGATATAATATCTATCAGAGGACATTACAAACCTTTGAAAATTATATTATCCTTTATCTGAAAGTATTTAAAAAACCTTTGATAAATATCTACCATTTATCAGAGGTTATTAAAAACCTTTGAAAAAGATAAAATATTTTTCAAAGGACATTAAAAACCTTTGAAAATTATATTATCCTTTATCGAAGGTATTTAAAAAACCTTTGATAAATATCTACCTTTTATCGAAGGTTATTAAAAACCTTTGAAAAAGATATTATATTTATCAAAGAACATAAAAAACCTTTGAAAATTGTATTATCCTTTATCAAATGTATTTAAAAAACCTTTGATAAATATCCACCATTTATCGAAGGTTATAAAAAATCTTTCAAAAAGATGCATATTTATCAGAGGACATTAAAAACGTTTGAAAATTATATTATTCTTTATCAAAGGTTTTGAAAAAAAAAAAAAAACTTTACAAATATATACTTTTTCTGGAAGGTTATTAAAATTCTTTGAAAACTTTATTGTCCTTTTATGAAAGGTATTTAGAAGGCCTTTGAAATATATATAAGAAATCAATGGAAAATTATTGTCCAATATTTACCTTTTATCGAAAGTTATCAAAAACCTTCAATAAAAATACTATGTTTATACCATCAAAATCCTTAAATTCATGAACTTATTAATAAAAAAAAAAAAAGACCGATTTTTTACTTTTTATCAAGGTTTTTAGAAACCTTCGGTAAAGATGTTTATGAAAAAAAATATTATTCTTTATCAAGATATTTAAAAAACCAGCAGCTTTGAGAAATATCTTTTTTCTTTTCTTTTTGGGTTAAAAAAAAATGAAGTTTATTTCTATTTTTTCTCCATCGGTGAAAGTGGGAAAATTGAATAGTCTGAGAAAGGGTAAATCCTCTTTGTTCGTCCTCGGTCATTCGTGGATCGTGACAATAGTTTGCAGGCTGACGTCAGCGATTCACCCCTCGGAGCTTACCTCAATTTCAATTGTTGCAAGGTAATCTTCATTCTTCTTCCATCTCACTCTCTAAACTCCCTCAATTTGAACTTCTAGTGACATCAATTAGCTGGACGAACAAAGCTAGTTTTTCCGGCAATCTGATCTAAAGCGGCATCGGGATTAGGTTTTGGTTTCTGTTTTGTTCTAATTTTTGTGTGGAATTGGTGTAATTGTTGGTGGAATTTTGTCTGTGTTTGATTTGGACTCCACTCGATTACATTCCAGGGATCATCTACACTCTCTATGCCATCGTCTTCGTCGAGTTTATGCCCCCAGAATTGGGTTTTGCTTTCTGTTTTGGGAACTTCGAGATTGCGAGATTGATGAAGGACAAGTAG

mRNA sequence

ATGTTTGTGGCCGGAGGATTTCCTCTCACACCTCCAACACCAATTGAGGATATCAACAACCCATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGGAAAGATCTGAAGTTCAAGCGCGTGGTGAATGGTCTGATACAGACTGGTATTCTGGCCGGGTTTCGTTCACTTAGAATATATGAAATTGTGGTGGAGGCTCAAACCACCAATGAAATTCTTTGGACATATGTGGCTAAGGGTGATGAGCAAGACAGGATCATCTACACTCTCTATGCCATCGTCTTCGTCGAGTTTATGCCCCCAGAATTGGGTTTTGCTTTCTGTTTTGGGAACTTCGAGATTGCGAGATTGATGAAGGACAAGTAG

Coding sequence (CDS)

ATGTTTGTGGCCGGAGGATTTCCTCTCACACCTCCAACACCAATTGAGGATATCAACAACCCATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGATCTGTGCAAGACATTGGAAGGTTTGCAGTGAATGAGTACAACAAGGAAAAAGGGAAAGATCTGAAGTTCAAGCGCGTGGTGAATGGTCTGATACAGACTGGTATTCTGGCCGGGTTTCGTTCACTTAGAATATATGAAATTGTGGTGGAGGCTCAAACCACCAATGAAATTCTTTGGACATATGTGGCTAAGGGTGATGAGCAAGACAGGATCATCTACACTCTCTATGCCATCGTCTTCGTCGAGTTTATGCCCCCAGAATTGGGTTTTGCTTTCTGTTTTGGGAACTTCGAGATTGCGAGATTGATGAAGGACAAGTAG

Protein sequence

MFVAGGFPLTPPTPIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTGILAGFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMPPELGFAFCFGNFEIARLMKDK

Homology

[Close]

BLAST of Lcy04g001500 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-06
Identity = 43/112 (38.39%), Postives = 56/112 (50.00%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTG 73
           PI DIN+P VQ +G  AV                  N Y+K  G+ LKF RVVNGL    
Sbjct: 12  PIIDINDPHVQALGELAVE-----------------NLYSKNSGEKLKFVRVVNGLKSDK 71

Query: 74  ILA-GFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMP 125
            +  GF  + +Y +V+EA+T  EI WTY  K +E   +   L   VF+ F P
Sbjct: 72  YIGPGFTEVILYHLVLEAKTNEEINWTYATKLEE---VSGGLIRHVFLSFEP 103

[Close]

BLAST of Lcy04g001500 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.6e-05
Identity = 41/114 (35.96%), Postives = 59/114 (51.75%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTG 73
           PI DIN+P VQ IG+ AV +++                 +   G+ LKF RVVNGL    
Sbjct: 12  PIIDINDPHVQIIGKLAVEDHD-----------------SHNPGEKLKFVRVVNGLKSDK 71

Query: 74  ILA-GFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYT--LYAIVFVEFMP 125
            +  GF  + +Y +V+EA+T  EI WTY  K ++    +Y+  L   VF+ F P
Sbjct: 72  YIGPGFTEVILYHLVLEAKTNEEINWTYATKLEK----VYSGCLIRHVFLSFEP 104

[Close]

BLAST of Lcy04g001500 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.5e-05
Identity = 42/121 (34.71%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQT- 73
           PIEDIN+              N E   VQD+G   V+++NK+ G++LKF RVVNGL  + 
Sbjct: 9   PIEDIND--------------NLE---VQDLGNLTVDDHNKKTGENLKFVRVVNGLAASF 68

Query: 74  ----GILAGFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMPPELG 130
                 L G     +YE+V+EA TT++I WTY A+  +   I    + +   +   P L 
Sbjct: 69  SDPISPLGGV----LYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLE 108

[Close]

BLAST of Lcy04g001500 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.5e-05
Identity = 42/121 (34.71%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQT- 73
           PIEDIN+              N E   VQD+G   V+++NK+ G++LKF RVVNGL  + 
Sbjct: 9   PIEDIND--------------NLE---VQDLGNLTVDDHNKKTGENLKFVRVVNGLAASF 68

Query: 74  ----GILAGFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMPPELG 130
                 L G     +YE+V+EA TT++I WTY A+  +   I    + +   +   P L 
Sbjct: 69  SDPISPLGGV----LYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLE 108

[Close]

BLAST of Lcy04g001500 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.8e-05
Identity = 30/64 (46.88%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 41  VQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTGILAGFRSLR-IYEIVVEAQTTNEILWT 100
           +Q +G FAV E+N+EKG++L+F +V+NG  Q     G R +R I+ IV+ A+  N I+WT
Sbjct: 22  IQGLGEFAVREHNEEKGENLEFVKVINGFEQ-----GRRGVRIIFTIVLSAKNCNGIIWT 80

Query: 101 YVAK 104
           Y AK
Sbjct: 82  YKAK 80

[Close]

BLAST of Lcy04g001500 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-06
Identity = 43/112 (38.39%), Postives = 56/112 (50.00%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTG 73
           PI DIN+P VQ +G  AV                  N Y+K  G+ LKF RVVNGL    
Sbjct: 12  PIIDINDPHVQALGELAVE-----------------NLYSKNSGEKLKFVRVVNGLKSDK 71

Query: 74  ILA-GFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMP 125
            +  GF  + +Y +V+EA+T  EI WTY  K +E   +   L   VF+ F P
Sbjct: 72  YIGPGFTEVILYHLVLEAKTNEEINWTYATKLEE---VSGGLIRHVFLSFEP 103

[Close]

BLAST of Lcy04g001500 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 58.9 bits (141), Expect = 4.1e-05
Identity = 37/96 (38.54%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 10  TPPTPIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGL 69
           +P  PI+DIN+P                   VQ+IG   V+++NK+ G++LKF RVVNGL
Sbjct: 5   SPFEPIKDINDP------------------QVQEIGNLTVDDHNKKTGENLKFVRVVNGL 64

Query: 70  IQTGILAGFRSL--RIYEIVVEAQTTNEILWTYVAK 104
              G +  F  L   +YE+V+EA    +I WTY  K
Sbjct: 65  F-AGFVDPFSPLGGTVYELVLEAINAGKINWTYKVK 81

[Close]

BLAST of Lcy04g001500 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 57.8 bits (138), Expect = 9.2e-05
Identity = 42/121 (34.71%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 14  PIEDINNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQT- 73
           PIEDIN+              N E   VQD+G   V+++NK+ G++LKF RVVNGL  + 
Sbjct: 9   PIEDIND--------------NLE---VQDLGNLTVDDHNKKTGENLKFVRVVNGLAASF 68

Query: 74  ----GILAGFRSLRIYEIVVEAQTTNEILWTYVAKGDEQDRIIYTLYAIVFVEFMPPELG 130
                 L G     +YE+V+EA TT++I WTY A+  +   I    + +   +   P L 
Sbjct: 69  SDPISPLGGV----LYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLE 108

[Close]

BLAST of Lcy04g001500 vs. NCBI nr
Match: KAA0040521.1 (Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-04
Identity = 30/64 (46.88%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 41  VQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTGILAGFRSLR-IYEIVVEAQTTNEILWT 100
           +Q +G FAV E+N+EKG++L+F +V+NG  Q     G R +R I+ IV+ A+  N I+WT
Sbjct: 22  IQGLGEFAVREHNEEKGENLEFVKVINGFEQ-----GRRGVRIIFTIVLSAKNCNGIIWT 80

Query: 101 YVAK 104
           Y AK
Sbjct: 82  YKAK 80

[Close]

BLAST of Lcy04g001500 vs. NCBI nr
Match: XP_038897049.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-04
Identity = 34/85 (40.00%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 18  INNPSVQDIGRFAVNEYNKEKGSVQDIGRFAVNEYNKEKGKDLKFKRVVNGLIQTGILAG 77
           I  P   ++GR+   E N E   VQ++GRF+V E+NK+ G +LKF RVVNG  +  ++AG
Sbjct: 16  IQGPKDVELGRWEPIE-NIEDPYVQEMGRFSVMEHNKQTGANLKFIRVVNG--EKQVVAG 75

Query: 78  FRSLRIYEIVVEAQTTNEILWTYVA 103
                 Y + +EA+    ++WTY A
Sbjct: 76  IN----YRLRIEARNEMNLVWTYEA 93

The following BLAST results are available for this feature:

BLAST of Lcy04g001500 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy04g001500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ98	1.4e-06	38.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
A0A0A0L191	2.6e-05	35.96	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]
A0A5A7TJK0	4.5e-05	34.71	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	4.5e-05	34.71	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]
A0A5A7TFU6	5.8e-05	46.88	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]

BLAST of Lcy04g001500 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN54823.1	2.9e-06	38.39	hypothetical protein Csa_012607 [Cucumis sativus]	[more]
KAE8649716.1	4.1e-05	38.54	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
XP_008442277.1	9.2e-05	34.71	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]
KAA0040521.1	1.2e-04	46.88	Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. maku...	[more]
XP_038897049.1	3.5e-04	40.00	cysteine proteinase inhibitor 5-like [Benincasa hispida]	[more]

BLAST of Lcy04g001500 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 11..39 e-value: 4.8E-5 score: 25.5
None	No IPR available	GENE3D	3.10.450.10		coord: 40..121 e-value: 3.9E-10 score: 41.6
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 13..39 coord: 40..103
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 40..101
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 40..102 e-value: 8.0E-8 score: 32.5
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 17..94 e-value: 4.65427E-6 score: 40.9204
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 40..103 coord: 13..39

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy04g001500.1	Lcy04g001500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity