Lcy03g011400 (gene) Sponge gourd (P93075) v1

Overview
NameLcy03g011400
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPhenylalanine ammonia-lyase
LocationChr03: 45345523 .. 45348167 (+)
RNA-Seq ExpressionLcy03g011400
SyntenyLcy03g011400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAGTTATTAAAGAGAGCCTAAATGGTCATCAATTAAGCCTAAAACTATTCTATTTTTGGATTTTGAAAACCAAACACATTAACTTTCTTTTAGAAATAATCAAAATCCAAACGATGCTCGAGGAGATAACTCAACTCAAGTCAAATGCTCATTTGCCCATGAAAAAAAACTAAGAAACTACCATGCAAAATACAAAAGAAAAATGAAAAAGATATCTTTTCCCATGCAAGGGAAGCTTCTAATTTTTGTAACTTTCCTCAGAACTTCATCTTCAACTTCGGCTACAAGTTAGAAATCAATCATCAAAATTTGCTATAAATTCATGCCCTTTTCTTCCTCCAAAAGCCACAGCTCCTCCAATAGTCTGAACTTTCCTGCATTCTCACTTAGTTACTCGTTCTTCCTTCTCCAATGGCCAACTCTGGATTTTTCTGCTCCTCAACTCTCTTAGAAACTGACTCTGTTCCTCAACCAAAATCCTTTTCCTCTGTTTCGGCCTCCGACCATTGGGTCAAGGCTGCCAACGCTCTCAGCCACACCCATTTTGAGGAGGTTTGTCGCATGGTATCGGAGTTTCAAGAGGCTCAAACTGTTGACATCGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCCATTGCTCGTCGCAGCGATGTCAAAGTTAACCTGAACGAAGCCGTGGCCCGTGATCGTGTGGCCAAAAGTGCAAATTGGGTTGCTGAGAAAATCTCTTGTGGGATCGATGTATATGGAGTTACCACTGGCTTTGGCGCCACCTCCCACAGGCGTACGACCAAGACAGCCGATCTCCAGACAGAGCTCATTAGGTTCCTCAACGCCGGAGTCATAGGAAAGGACAGTCTTCCATCTAGCTACTCAAAGGCAGCCATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCGGGCATTCGTTGGGAAATTCTTGAAGCTGTGGTGAAGCTCATGAATCAGAATTTGATCCCTAAATTGCCTTTGAGAGGGACGATCACAGCTTCAGGAGATCTTGTTCCACTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCACAATTCCAAGGTTACCACCATGGAAGGCGACGAGATCACAGCCATGGAAGCTCTGAAGAGAGCTGGAATTGGAGGCCCATTTGAGCTTCAAGCAAAGGAAGGCTTAGCTCTTGTCAATGGAACAGCCGTCGGGTCTGCCGTCGCCGCCACAGTCTGCTATGATGCCAATATTTTGGCTCTTTTAGCCGTCGTTCTCTCTGCTTTGTTCTGTGAGGCCATGCTCGGCAAGCCAGAATTTGCAGACCCATTGACCCATAAGCTCAAGCACCACCCGGGCCAGATTGAATCAGCCGCAGCCATGGAGTTTCTCTTGAACCAAAGCGACTTTATGAAAGAAGCAAAAATCCGCCATGAAAATGACCCATTGACAAAGCCAAAACAAGACCGCTATGCACTTCGAACTTCTCCTCAATGGCTCGGGCCCCAAATCGAAGTCATTCGAACGGCCACCCATTCGATCGAAAGGGAAATCAACTCTGTAAATGATAACCCACTAATCGACGTCAATCGGGACTTAGCTCTACATGGAGGAAACTTTCAGGGGACACCAATCGGCGTCTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAACTGATGTTCGCCCAATTCTCTGAGCTCGTCTGCGATCATTACAACAATGGATTGCCATCTAATCTGAGCGGCGGGCCAAACCCTAGCTTGGATTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAGTACTTAGCAAACCCTGTCACAACCCATGTCCAGAGTGCAGAGCAACACAATCAAGACGTAAACTCTCTAGGTCTAATCTCAGCCAGAAAATCCGCCGAAGCCATTGAAATTCTCAAGCTCATGTCAGCTACATACATGGTGGCGCTCTGCCAGGCGATCGATTTAAGGCAATACGAAGAAAACATGAGGGAAGTTGTGAAAAACGCGCTTCTACAAACCGTAAGAAGAACCCTATATGTTGCAGAGTACGGATCGCTTCTCGAATCACGATTCTGCGAGAAAGATCTCCTGCAAGTAATCGAGCGCGAGCCAGTCTTCTCCTACATCGACGATCCCGCCAACACTTCCTACGCCCTAATCCCTAAATTACTGGAAGTTGTAGTGGAAAGAGCGTTGAAAGGCGTTGCAGCAGCGAAAAGCGAGAATGGAGAGGAAACAGGAATCGCCATTTTCAAGCAAATCCCAGGGTTTCTAGAGGAATTAAAGGCGAAACTGGAAAAAGATGTTGAAACTGCGAGAGGGAGATTCGAGAATGGTGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGCACTTACCCGATCTACCGCTTTGTGAGGACTGAAGTGGGAACTCAACTGCTCAGCGGGGCGAAGAAGGTGAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAAGCCATTAATGAAGGGAAGCTTGGAGATGTTCTGATCAACTGTTTGAAGAATTAGGGCAATTGCAATTTTTATTGAGAAGTATGAAAGTTTTTTTTTTTTTTTTTTTTATTCAACAATTACTAGTGTGGGAATAAAATCATGTAATTTGA

mRNA sequence

AAATAGTTATTAAAGAGAGCCTAAATGGTCATCAATTAAGCCTAAAACTATTCTATTTTTGGATTTTGAAAACCAAACACATTAACTTTCTTTTAGAAATAATCAAAATCCAAACGATGCTCGAGGAGATAACTCAACTCAAGTCAAATGCTCATTTGCCCATGAAAAAAAACTAAGAAACTACCATGCAAAATACAAAAGAAAAATGAAAAAGATATCTTTTCCCATGCAAGGGAAGCTTCTAATTTTTGTAACTTTCCTCAGAACTTCATCTTCAACTTCGGCTACAAGTTAGAAATCAATCATCAAAATTTGCTATAAATTCATGCCCTTTTCTTCCTCCAAAAGCCACAGCTCCTCCAATAGTCTGAACTTTCCTGCATTCTCACTTAGTTACTCGTTCTTCCTTCTCCAATGGCCAACTCTGGATTTTTCTGCTCCTCAACTCTCTTAGAAACTGACTCTGTTCCTCAACCAAAATCCTTTTCCTCTGTTTCGGCCTCCGACCATTGGGTCAAGGCTGCCAACGCTCTCAGCCACACCCATTTTGAGGAGGTTTGTCGCATGGTATCGGAGTTTCAAGAGGCTCAAACTGTTGACATCGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCCATTGCTCGTCGCAGCGATGTCAAAGTTAACCTGAACGAAGCCGTGGCCCGTGATCGTGTGGCCAAAAGTGCAAATTGGGTTGCTGAGAAAATCTCTTGTGGGATCGATGTATATGGAGTTACCACTGGCTTTGGCGCCACCTCCCACAGGCGTACGACCAAGACAGCCGATCTCCAGACAGAGCTCATTAGGTTCCTCAACGCCGGAGTCATAGGAAAGGACAGTCTTCCATCTAGCTACTCAAAGGCAGCCATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCGGGCATTCGTTGGGAAATTCTTGAAGCTGTGGTGAAGCTCATGAATCAGAATTTGATCCCTAAATTGCCTTTGAGAGGGACGATCACAGCTTCAGGAGATCTTGTTCCACTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCACAATTCCAAGGTTACCACCATGGAAGGCGACGAGATCACAGCCATGGAAGCTCTGAAGAGAGCTGGAATTGGAGGCCCATTTGAGCTTCAAGCAAAGGAAGGCTTAGCTCTTGTCAATGGAACAGCCGTCGGGTCTGCCGTCGCCGCCACAGTCTGCTATGATGCCAATATTTTGGCTCTTTTAGCCGTCGTTCTCTCTGCTTTGTTCTGTGAGGCCATGCTCGGCAAGCCAGAATTTGCAGACCCATTGACCCATAAGCTCAAGCACCACCCGGGCCAGATTGAATCAGCCGCAGCCATGGAGTTTCTCTTGAACCAAAGCGACTTTATGAAAGAAGCAAAAATCCGCCATGAAAATGACCCATTGACAAAGCCAAAACAAGACCGCTATGCACTTCGAACTTCTCCTCAATGGCTCGGGCCCCAAATCGAAGTCATTCGAACGGCCACCCATTCGATCGAAAGGGAAATCAACTCTGTAAATGATAACCCACTAATCGACGTCAATCGGGACTTAGCTCTACATGGAGGAAACTTTCAGGGGACACCAATCGGCGTCTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAACTGATGTTCGCCCAATTCTCTGAGCTCGTCTGCGATCATTACAACAATGGATTGCCATCTAATCTGAGCGGCGGGCCAAACCCTAGCTTGGATTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAGTACTTAGCAAACCCTGTCACAACCCATGTCCAGAGTGCAGAGCAACACAATCAAGACGTAAACTCTCTAGGTCTAATCTCAGCCAGAAAATCCGCCGAAGCCATTGAAATTCTCAAGCTCATGTCAGCTACATACATGGTGGCGCTCTGCCAGGCGATCGATTTAAGGCAATACGAAGAAAACATGAGGGAAGTTGTGAAAAACGCGCTTCTACAAACCGTAAGAAGAACCCTATATGTTGCAGAGTACGGATCGCTTCTCGAATCACGATTCTGCGAGAAAGATCTCCTGCAAGTAATCGAGCGCGAGCCAGTCTTCTCCTACATCGACGATCCCGCCAACACTTCCTACGCCCTAATCCCTAAATTACTGGAAGTTGTAGTGGAAAGAGCGTTGAAAGGCGTTGCAGCAGCGAAAAGCGAGAATGGAGAGGAAACAGGAATCGCCATTTTCAAGCAAATCCCAGGGTTTCTAGAGGAATTAAAGGCGAAACTGGAAAAAGATGTTGAAACTGCGAGAGGGAGATTCGAGAATGGTGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGCACTTACCCGATCTACCGCTTTGTGAGGACTGAAGTGGGAACTCAACTGCTCAGCGGGGCGAAGAAGGTGAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAAGCCATTAATGAAGGGAAGCTTGGAGATGTTCTGATCAACTGTTTGAAGAATTAGGGCAATTGCAATTTTTATTGAGAAGTATGAAAGTTTTTTTTTTTTTTTTTTTTATTCAACAATTACTAGTGTGGGAATAAAATCATGTAATTTGA

Coding sequence (CDS)

ATGGCCAACTCTGGATTTTTCTGCTCCTCAACTCTCTTAGAAACTGACTCTGTTCCTCAACCAAAATCCTTTTCCTCTGTTTCGGCCTCCGACCATTGGGTCAAGGCTGCCAACGCTCTCAGCCACACCCATTTTGAGGAGGTTTGTCGCATGGTATCGGAGTTTCAAGAGGCTCAAACTGTTGACATCGAAGGCACCACCCTCACTGTTGCTCAGGTCACCGCCATTGCTCGTCGCAGCGATGTCAAAGTTAACCTGAACGAAGCCGTGGCCCGTGATCGTGTGGCCAAAAGTGCAAATTGGGTTGCTGAGAAAATCTCTTGTGGGATCGATGTATATGGAGTTACCACTGGCTTTGGCGCCACCTCCCACAGGCGTACGACCAAGACAGCCGATCTCCAGACAGAGCTCATTAGGTTCCTCAACGCCGGAGTCATAGGAAAGGACAGTCTTCCATCTAGCTACTCAAAGGCAGCCATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCGGGCATTCGTTGGGAAATTCTTGAAGCTGTGGTGAAGCTCATGAATCAGAATTTGATCCCTAAATTGCCTTTGAGAGGGACGATCACAGCTTCAGGAGATCTTGTTCCACTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCACAATTCCAAGGTTACCACCATGGAAGGCGACGAGATCACAGCCATGGAAGCTCTGAAGAGAGCTGGAATTGGAGGCCCATTTGAGCTTCAAGCAAAGGAAGGCTTAGCTCTTGTCAATGGAACAGCCGTCGGGTCTGCCGTCGCCGCCACAGTCTGCTATGATGCCAATATTTTGGCTCTTTTAGCCGTCGTTCTCTCTGCTTTGTTCTGTGAGGCCATGCTCGGCAAGCCAGAATTTGCAGACCCATTGACCCATAAGCTCAAGCACCACCCGGGCCAGATTGAATCAGCCGCAGCCATGGAGTTTCTCTTGAACCAAAGCGACTTTATGAAAGAAGCAAAAATCCGCCATGAAAATGACCCATTGACAAAGCCAAAACAAGACCGCTATGCACTTCGAACTTCTCCTCAATGGCTCGGGCCCCAAATCGAAGTCATTCGAACGGCCACCCATTCGATCGAAAGGGAAATCAACTCTGTAAATGATAACCCACTAATCGACGTCAATCGGGACTTAGCTCTACATGGAGGAAACTTTCAGGGGACACCAATCGGCGTCTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAACTGATGTTCGCCCAATTCTCTGAGCTCGTCTGCGATCATTACAACAATGGATTGCCATCTAATCTGAGCGGCGGGCCAAACCCTAGCTTGGATTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAGTACTTAGCAAACCCTGTCACAACCCATGTCCAGAGTGCAGAGCAACACAATCAAGACGTAAACTCTCTAGGTCTAATCTCAGCCAGAAAATCCGCCGAAGCCATTGAAATTCTCAAGCTCATGTCAGCTACATACATGGTGGCGCTCTGCCAGGCGATCGATTTAAGGCAATACGAAGAAAACATGAGGGAAGTTGTGAAAAACGCGCTTCTACAAACCGTAAGAAGAACCCTATATGTTGCAGAGTACGGATCGCTTCTCGAATCACGATTCTGCGAGAAAGATCTCCTGCAAGTAATCGAGCGCGAGCCAGTCTTCTCCTACATCGACGATCCCGCCAACACTTCCTACGCCCTAATCCCTAAATTACTGGAAGTTGTAGTGGAAAGAGCGTTGAAAGGCGTTGCAGCAGCGAAAAGCGAGAATGGAGAGGAAACAGGAATCGCCATTTTCAAGCAAATCCCAGGGTTTCTAGAGGAATTAAAGGCGAAACTGGAAAAAGATGTTGAAACTGCGAGAGGGAGATTCGAGAATGGTGATTTCGCAGTGGAAAACAGAATCAAGAAATGCAGCACTTACCCGATCTACCGCTTTGTGAGGACTGAAGTGGGAACTCAACTGCTCAGCGGGGCGAAGAAGGTGAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAAGCCATTAATGAAGGGAAGCTTGGAGATGTTCTGATCAACTGTTTGAAGAATTAG

Protein sequence

MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN
Homology
BLAST of Lcy03g011400 vs. ExPASy Swiss-Prot
Match: P52777 (Phenylalanine ammonia-lyase OS=Pinus taeda OX=3352 GN=PAL PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 2.9e-248
Identity = 442/688 (64.24%), Postives = 537/688 (78.05%), Query Frame = 0

Query: 25  SSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARRSDVKV 84
           SS S   +WV+AA A+  +HFEEV  MV  +  A+ + IEG +LT++ V A+ARRS VKV
Sbjct: 26  SSGSDPLNWVRAAKAMEGSHFEEVKAMVDSYFGAKEISIEGKSLTISDVAAVARRSQVKV 85

Query: 85  NLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAG 144
            L+ A A+ RV +S+NWV  +++ G D YGVTTGFGATSHRRT + A+LQ ELIRFLNAG
Sbjct: 86  KLDAAAAKSRVEESSNWVLTQMTKGTDTYGVTTGFGATSHRRTNQGAELQKELIRFLNAG 145

Query: 145 VIGK---DSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTIT 204
           V+GK   + L    ++AAMLVRTNTL+QGYSG+RW+ILE V KL+N  L PKLPLRGTIT
Sbjct: 146 VLGKCPENVLSEDTTRAAMLVRTNTLLQGYSGVRWDILETVEKLLNAWLTPKLPLRGTIT 205

Query: 205 ASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIGGPFELQAKEGLALVNGT 264
           ASGDLVPLSYIAG++TGR NS+V + +G E++  EALK+ G+  PFELQ KEGLA+VNGT
Sbjct: 206 ASGDLVPLSYIAGLLTGRPNSRVRSRDGIEMSGAEALKKVGLEKPFELQPKEGLAIVNGT 265

Query: 265 AVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAME 324
           +VG+A+A+ VC+DAN+LALL+ V+SA+FCE M GKPEF DPLTHKLKHHPGQ+E+AA ME
Sbjct: 266 SVGAALASIVCFDANVLALLSEVISAMFCEVMNGKPEFTDPLTHKLKHHPGQMEAAAIME 325

Query: 325 FLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVN 384
           ++L+ S +MK A   HE +PL KPKQDRY LRTSPQWLGPQ+E+IR+ATH IEREINSVN
Sbjct: 326 YVLDGSSYMKHAAKLHEMNPLQKPKQDRYGLRTSPQWLGPQVEIIRSATHMIEREINSVN 385

Query: 385 DNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSN 444
           DNP+IDV RD ALHGGNFQGTPIGVSMDNLR++I+AIGKLMFAQFSELV D+YN GLPSN
Sbjct: 386 DNPVIDVARDKALHGGNFQGTPIGVSMDNLRLSISAIGKLMFAQFSELVNDYYNGGLPSN 445

Query: 445 LSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSA 504
           LSGGPNPSLDYG KGAEIAMA+Y SEL YLANPVT+HVQSAEQHNQDVNSLGL+SARKSA
Sbjct: 446 LSGGPNPSLDYGLKGAEIAMASYTSELLYLANPVTSHVQSAEQHNQDVNSLGLVSARKSA 505

Query: 505 EAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFC 564
           EAI+ILKLM +TY+ ALCQA+DLR  EENM   VK  + Q  ++TL     G LL  RFC
Sbjct: 506 EAIDILKLMLSTYLTALCQAVDLRHLEENMLATVKQIVSQVAKKTLSTGLNGELLPGRFC 565

Query: 565 EKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFK 624
           EKDLLQV++ E VFSYIDDP N SY L  KL  ++VE A K     K  N      +IF 
Sbjct: 566 EKDLLQVVDNEHVFSYIDDPCNASYPLTQKLRNILVEHAFKNAEGEKDPN-----TSIFN 625

Query: 625 QIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAK 684
           +IP F  ELKA+LE  V  AR  ++ G   + +RI++C +YP+Y FVR ++GT+LLSG +
Sbjct: 626 KIPVFEAELKAQLEPQVSLARESYDKGTSPLPDRIQECRSYPLYEFVRNQLGTKLLSGTR 685

Query: 685 KVSPGEDIEKVVEAINEGKLGDVLINCL 710
            +SPGE IE V +AI+E K+   L  CL
Sbjct: 686 TISPGEVIEVVYDAISEDKVIVPLFKCL 708

BLAST of Lcy03g011400 vs. ExPASy Swiss-Prot
Match: A0A2H5AIW0 (Phenylalanine ammonia-lyase 1 OS=Narcissus pseudonarcissus OX=39639 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 1.0e-240
Identity = 441/686 (64.29%), Postives = 533/686 (77.70%), Query Frame = 0

Query: 33  WVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIAR-RSDVKVNLNEAVA 92
           W  AA AL+ +H EEV RMV E +EA  + + G TL V Q+ A+A   S  KV L+E+ A
Sbjct: 21  WGAAAEALAGSHLEEVKRMVKEHREA-CIRLGGATLKVGQIAAVANGGSAAKVELDES-A 80

Query: 93  RDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK--- 152
           R RV  S++WV + +S G D YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G    
Sbjct: 81  RARVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGRD 140

Query: 153 --DSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGDL 212
             ++LP+S ++AAMLVR NTL+QGYSGIR+EILEA+ +L+N N+ P LPLRGTITASGDL
Sbjct: 141 SCNTLPASTTRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNVTPCLPLRGTITASGDL 200

Query: 213 VPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGI-GGPFELQAKEGLALVNGTAVGS 272
           VPLSYIAG++TGR NSK T  +G +I A EA + AGI  G F+LQ KEGLALVNGTAVGS
Sbjct: 201 VPLSYIAGILTGRPNSKATAPDGAQIDASEAFRLAGITDGFFQLQPKEGLALVNGTAVGS 260

Query: 273 AVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLN 332
            +A+TV YDANILA+L+ V SA+FCE M GKPEF D LTHKLKHHPGQIE+AA ME +L+
Sbjct: 261 GLASTVLYDANILAVLSEVFSAIFCEVMQGKPEFTDHLTHKLKHHPGQIEAAAVMEHILD 320

Query: 333 QSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPL 392
            S +MK AK  HE DPL KPKQDRYALRTSPQWLGPQIEVIR+AT SIEREINSVNDNPL
Sbjct: 321 GSSYMKMAKKLHELDPLQKPKQDRYALRTSPQWLGPQIEVIRSATKSIEREINSVNDNPL 380

Query: 393 IDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGG 452
           IDV+R+ ALHGGNFQGTPIGVSMDN R+AIA+IGKLMFAQFSELV D YNNGLPSNLSGG
Sbjct: 381 IDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGG 440

Query: 453 PNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIE 512
            +PSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLISARK+ EA+ 
Sbjct: 441 RDPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISARKTEEAVH 500

Query: 513 ILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKDL 572
           ILKLMS T++V LCQAIDLR  EEN++  VK  + Q  +R L     G L  SRFCEKDL
Sbjct: 501 ILKLMSTTFLVGLCQAIDLRHLEENLKSTVKTTISQVAKRVLTSGINGELHPSRFCEKDL 560

Query: 573 LQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIPG 632
           ++V++RE VFSY+DDP + +Y L+ +L +++VE A+        E  ++   +IF++I  
Sbjct: 561 IKVVDREHVFSYVDDPCSATYPLMQRLRQILVEHAMN-----NGEKEKDADTSIFRKISA 620

Query: 633 FLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVSP 692
           F +ELK  L K+VE+AR  +ENG  A++NRI++C +YP+YRFVR E GT LL+G    SP
Sbjct: 621 FEDELKVVLPKEVESARVAYENGTSAIKNRIEECRSYPLYRFVREETGTSLLTGENVRSP 680

Query: 693 GEDIEKVVEAINEGKLGDVLINCLKN 712
           GE+ +KV  AI EG+L D L+ CL++
Sbjct: 681 GEEFDKVFNAICEGRLVDPLLECLED 699

BLAST of Lcy03g011400 vs. ExPASy Swiss-Prot
Match: Q50EX7 (Phenylalanine ammonia-lyase 1 OS=Petunia hybrida OX=4102 GN=PAL1 PE=1 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 2.2e-240
Identity = 444/687 (64.63%), Postives = 539/687 (78.46%), Query Frame = 0

Query: 32  HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARRS--DVKVNLNEA 91
           +W  AA+AL  +H +EV RMV EF++   V + G TLTVAQV AIA +S  DV V L+EA
Sbjct: 29  NWNMAADALKGSHLDEVKRMVKEFRK-PVVRLGGETLTVAQVAAIASQSGTDVTVQLSEA 88

Query: 92  VARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIG-- 151
            +R  V  S++WV + +  G D YGVTTGFGATSHRRT + A LQ ELIRFLNAG+ G  
Sbjct: 89  -SRAGVKASSDWVLQGMINGTDSYGVTTGFGATSHRRTKEGAALQKELIRFLNAGIFGNG 148

Query: 152 ---KDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASG 211
                +LP S ++AAMLVR NTL+QGYSGIR+EILEA+ KL+N N+ P LPLRGTITASG
Sbjct: 149 TESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITASG 208

Query: 212 DLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIG-GPFELQAKEGLALVNGTAV 271
           DLVPLSYIAG++TGR NSK T   G+ + A++A +RAGI  G FELQ KEGLALVNGTAV
Sbjct: 209 DLVPLSYIAGLLTGRPNSKATGPNGELLDAVKAFQRAGIDTGFFELQPKEGLALVNGTAV 268

Query: 272 GSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFL 331
           GS +A+ V +DANILA+L+ VLSA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +
Sbjct: 269 GSGLASMVLFDANILAVLSEVLSAIFAEVMQGKPEFTDYLTHKLKHHPGQIEAAAIMEHI 328

Query: 332 LNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDN 391
           L+ S ++KEAKI HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT  IEREINSVNDN
Sbjct: 329 LDGSSYVKEAKIVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDN 388

Query: 392 PLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLS 451
           PLIDV+R+ ALHGGNFQGTPIGVSMDN R+A+A+IGKLMFAQFSELV D+YNNGLPSNL+
Sbjct: 389 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLMFAQFSELVNDYYNNGLPSNLT 448

Query: 452 GGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEA 511
           GG NPSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA
Sbjct: 449 GGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEA 508

Query: 512 IEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEK 571
           ++ILKLMS+TY+VALCQAIDLR  EEN++  VKN++    ++ L + E G L  SRFCEK
Sbjct: 509 VDILKLMSSTYLVALCQAIDLRHLEENLKATVKNSVSLVAKKVLTIGEKGELHHSRFCEK 568

Query: 572 DLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQI 631
           D+L+V++RE +F+Y DD  + +Y L+ KL +V+V+RAL  V   K     ++  +IF++I
Sbjct: 569 DMLKVVDREYIFAYADDACSATYPLMQKLRQVLVDRALLNVDGEK-----DSSTSIFQKI 628

Query: 632 PGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKV 691
             F EELK  L K++E AR   E G  A+ NRI++C +YP+Y+FVR E+    L+G K  
Sbjct: 629 KAFEEELKVVLPKEIERARSDLEQGKPAIPNRIQECRSYPLYKFVREELKANYLTGEKVQ 688

Query: 692 SPGEDIEKVVEAINEGKLGDVLINCLK 711
           SPGE+ +KV  A+NEGKL D L+NCLK
Sbjct: 689 SPGEEFDKVFTAMNEGKLVDPLLNCLK 708

BLAST of Lcy03g011400 vs. ExPASy Swiss-Prot
Match: P45726 (Phenylalanine ammonia-lyase OS=Camellia sinensis OX=4442 GN=PAL PE=2 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 3.2e-239
Identity = 437/686 (63.70%), Postives = 539/686 (78.57%), Query Frame = 0

Query: 32  HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIA-RRSDVKVNLNEAV 91
           +W  AA A+  +H EEV  MV EF++   V + G TLT++QV AIA R S+V V L+E+ 
Sbjct: 26  NWGVAAEAMKGSHLEEVKGMVEEFRK-PVVRLGGETLTISQVAAIAVRGSEVAVELSES- 85

Query: 92  ARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK-- 151
           AR+ V  S++WV E ++ G D YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G   
Sbjct: 86  AREGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKEGGALQKELIRFLNAGIFGNGT 145

Query: 152 ---DSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGD 211
               +LP S ++AAMLVR NTL+QGYSGIR+EILEA+ K +N N+ P LPLRGTITASGD
Sbjct: 146 ESCHTLPQSATRAAMLVRINTLLQGYSGIRFEILEAISKFLNNNITPCLPLRGTITASGD 205

Query: 212 LVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGI-GGPFELQAKEGLALVNGTAVG 271
           LVPLSYIAG++TGRHNSK     G+ +   EA + AG+ GG FELQ KEGLALVNGTAVG
Sbjct: 206 LVPLSYIAGLLTGRHNSKAVGPTGEILHPKEAFRLAGVEGGFFELQPKEGLALVNGTAVG 265

Query: 272 SAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLL 331
           S +A+ V ++ANILA+L+ VLSA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +L
Sbjct: 266 SGLASMVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHIL 325

Query: 332 NQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNP 391
           + S ++K A+  HE DPL KPKQDRYALRTSPQWLGP IEVIR++T SIEREINSVNDNP
Sbjct: 326 DGSSYVKAAQKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRSSTKSIEREINSVNDNP 385

Query: 392 LIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSG 451
           LI+V+R+ ALHGGNFQGTPIGVSMDN R+A+A+IGKLMFAQFSELV D YNNGLPSNLSG
Sbjct: 386 LINVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSNLSG 445

Query: 452 GPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI 511
           G NPSLDYGFKGAEIAMAAYCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA+
Sbjct: 446 GRNPSLDYGFKGAEIAMAAYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAV 505

Query: 512 EILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKD 571
           +ILKLMS+TY+VALCQA+DLR +EEN+R  VK+ + Q  +R L +   G L  SRFCEKD
Sbjct: 506 DILKLMSSTYLVALCQAVDLRHFEENLRNTVKSTVSQVAKRVLTMGVNGELHPSRFCEKD 565

Query: 572 LLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIP 631
           LL+V++RE +F+YIDDP + +Y L+ KL +V+VE ALK       E+ +    +IF++I 
Sbjct: 566 LLRVVDREYIFAYIDDPCSATYPLMQKLRQVLVEHALK-----NGESEKNLSTSIFQKIR 625

Query: 632 GFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVS 691
            F EE+K  L K+VE+ R   ENG+ A+ NRIK+C +YP+Y+FVR E+GT+LL+G K  S
Sbjct: 626 AFEEEIKTLLPKEVESTRAAIENGNSAIPNRIKECRSYPLYKFVREELGTELLTGEKVRS 685

Query: 692 PGEDIEKVVEAINEGKLGDVLINCLK 711
           PGE+ +KV  A+ +G++ D L++CLK
Sbjct: 686 PGEEFDKVFTALCKGEMIDPLMDCLK 704

BLAST of Lcy03g011400 vs. ExPASy Swiss-Prot
Match: O23865 (Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 6.1e-238
Identity = 436/686 (63.56%), Postives = 536/686 (78.13%), Query Frame = 0

Query: 32  HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVA 91
           +W  AA AL+ +H +EV RMV+EF++   V + G TLTV+QV AIA  S VKV L E+ A
Sbjct: 22  NWGMAAEALTGSHLDEVKRMVAEFRKPM-VQLGGETLTVSQVAAIAAGS-VKVELAES-A 81

Query: 92  RDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKDS- 151
           R  V  S++WV E ++ G D YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G  + 
Sbjct: 82  RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGND 141

Query: 152 ----LPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGDL 211
               LP S ++AAMLVR NTL+QGYSGIR+EILEA+ K +NQN+ P LPLRGTITASGDL
Sbjct: 142 SSNILPHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNQNITPCLPLRGTITASGDL 201

Query: 212 VPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGI-GGPFELQAKEGLALVNGTAVGS 271
           VPLSYIAG++TGR NSK     G+ +TA EA K AG+ GG FELQ KEGLALVNGTAVGS
Sbjct: 202 VPLSYIAGLLTGRPNSKAVGPTGENLTAAEAFKLAGVDGGFFELQPKEGLALVNGTAVGS 261

Query: 272 AVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLN 331
            +A+ V ++ NILA+LA V+SA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +L+
Sbjct: 262 GMASMVLFETNILAVLAEVMSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILD 321

Query: 332 QSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPL 391
            S ++K A+ +HE DPL KPKQDRYALRTSPQWLGPQIEVIR++T  IEREINSVNDNPL
Sbjct: 322 GSSYVKAAEKQHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRSSTKMIEREINSVNDNPL 381

Query: 392 IDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGG 451
           IDV+R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNGLPSNLSGG
Sbjct: 382 IDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGG 441

Query: 452 PNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIE 511
            NPSLDYGFKGAEIAMA+YCSELQ+L NPVT HVQSAEQHNQDVNSLGLIS+RK+AEA+E
Sbjct: 442 RNPSLDYGFKGAEIAMASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVE 501

Query: 512 ILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKDL 571
           ILKLMS T++V LCQA+DLR  EEN++  VKN + Q  ++ L +   G L  SRFCE DL
Sbjct: 502 ILKLMSTTFLVGLCQAVDLRHLEENLKSTVKNTVSQVAKKVLTMGVNGELHPSRFCELDL 561

Query: 572 LQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIPG 631
           L+V++RE +F+YIDDP + +Y L+ KL +V+VE ALK       E  +    +IF++I  
Sbjct: 562 LRVVDREYIFAYIDDPCSATYPLMQKLRQVLVEHALK-----NGETEKNLSTSIFQKIAA 621

Query: 632 FLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVSP 691
           F +ELKA L K+VE+AR   E+G+ A+ NRIK+C +YP+Y+F+R E+GT  L+G K  SP
Sbjct: 622 FEDELKALLPKEVESARAVVESGNPAIPNRIKECRSYPLYKFIREELGTVYLTGEKVTSP 681

Query: 692 GEDIEKVVEAINEGKLGDVLINCLKN 712
           GE+ +KV  A+++G++ D L+ CL++
Sbjct: 682 GEEFDKVFTAMSKGEIIDPLLACLES 699

BLAST of Lcy03g011400 vs. ExPASy TrEMBL
Match: A0A6J1JJE5 (Phenylalanine ammonia-lyase OS=Cucurbita maxima OX=3661 GN=LOC111485128 PE=3 SV=1)

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 681/711 (95.78%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFCSS L + DSVPQ KSFSSVS SD+W KAANALSHTH EEVCRM+SEFQ+ + 
Sbjct: 1   MANSEFFCSSPLYQPDSVPQSKSFSSVSPSDYWNKAANALSHTHLEEVCRMLSEFQDTRA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRS+VKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSNVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEAL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYAL+PKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALMPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK+ENG ETG+AIFKQIPGFLEELK KLE DVETARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGVAIFKQIPGFLEELKEKLENDVETARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG+V++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGNVVLECLKN 711

BLAST of Lcy03g011400 vs. ExPASy TrEMBL
Match: A0A345BTD4 (Phenylalanine ammonia-lyase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 650/711 (91.42%), Postives = 678/711 (95.36%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFC S L + DSVPQ KSFS VS SD+W KAANALSHTH EEVCRM+SEFQ+A+ 
Sbjct: 1   MANSEFFCYSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEALKR
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALKR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYALIPKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK ENG ETGIAIFKQIPGFLEELK KLE DVE ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKKENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG V++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of Lcy03g011400 vs. ExPASy TrEMBL
Match: A0A6J1H8Z9 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111461649 PE=3 SV=1)

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 678/711 (95.36%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFCSS L + DSVPQ KSFS VS SD+W KAANALSHTH EEVCRM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEAL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYALIPKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK+ENG ETGIAIFKQIPGFLEELK KLE DVE ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG +++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSIVLECLKN 711

BLAST of Lcy03g011400 vs. ExPASy TrEMBL
Match: A0A6J1D395 (Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111016602 PE=3 SV=1)

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 644/711 (90.58%), Postives = 675/711 (94.94%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MA   FFCSS L   DS+ Q KSF SVSASDHWVKAA+AL   HFEEV RMVSEFQEA+T
Sbjct: 1   MATREFFCSSALSGADSIHQSKSFPSVSASDHWVKAADALRCPHFEEVRRMVSEFQEAKT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAE IS G D YGVTTGFG
Sbjct: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAENISRGTDTYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSHRRTTKTADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDNLPSIYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NLIPKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EG+E+TAM+ALKR
Sbjct: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGEEVTAMQALKR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AG+ GPFELQAKEGLALVNGTAVGSAVAATVC+DANILALLAVVLSA+FCEAMLGKPEFA
Sbjct: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAVVLSAMFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLL+QSDFMKEAK RHE DPLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLDQSDFMKEAKTRHEKDPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIRTATHSIEREINSVNDNPLIDV+RDLALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRTATHSIEREINSVNDNPLIDVHRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ+EENMREVVKNALL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQHEENMREVVKNALL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           Q VR+TLYVAE GSLLESRFCEKDLLQVIE E VFSYIDDP NTSYALIPKLLEV+VERA
Sbjct: 541 QAVRKTLYVAEDGSLLESRFCEKDLLQVIEHEAVFSYIDDPTNTSYALIPKLLEVLVERA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG +A K E+G ETG+ IF++IPGFLEELKAKLE++VE+ARGRFENGDFAVENRIKKCS
Sbjct: 601 LKGASADKGEHGNETGVTIFRRIPGFLEELKAKLEEEVESARGRFENGDFAVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIY FVRTEVGTQLLSGAK+VSPGEDIEKVV+AIN GK+G+V+  CLKN
Sbjct: 661 TYPIYHFVRTEVGTQLLSGAKRVSPGEDIEKVVDAINGGKIGEVVTKCLKN 711

BLAST of Lcy03g011400 vs. ExPASy TrEMBL
Match: A0A0A0LYZ4 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_1G590300 PE=3 SV=1)

HSP 1 Score: 1251.1 bits (3236), Expect = 0.0e+00
Identity = 639/711 (89.87%), Postives = 670/711 (94.23%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS F CSS   +T  VP  KS SS S SDHWVKAANALS THFEEVCRM+SEFQEAQT
Sbjct: 1   MANSQFLCSSARSKTGFVPLSKSVSSDSTSDHWVKAANALSQTHFEEVCRMLSEFQEAQT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VD+EGTTLTVAQV  IARRSDVKV LNE+VA DRVA+SA WVAEK+S GID+YGVTTGFG
Sbjct: 61  VDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVAESAKWVAEKVSSGIDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSH RTTK +DLQTELIRFLNAGVIGKD+LPS YSK AMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 ATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYSKVAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           A+VKLMN+NLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTT+EGDEITA+EALKR
Sbjct: 181 ALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTIEGDEITAIEALKR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AG+ GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLA VLSALFCEAMLGKPEF 
Sbjct: 241 AGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLASVLSALFCEAMLGKPEFT 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAA MEFLLNQSDFMKEAKI +ENDPLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKICNENDPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+RD+A+HGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVC HYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ+EENMREVVKNALL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQFEENMREVVKNALL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+R+TLY+AEYGSLLESRFCEKDLLQVIERE VFSYIDDP NTSY LIPKLLEV+VE+A
Sbjct: 541 QTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFSYIDDPTNTSYTLIPKLLEVLVEKA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKGVA  K++NG ETGIAIFKQIP FLEELKAKLEKDVE ARGRFENGDF +ENRIKKCS
Sbjct: 601 LKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEKDVENARGRFENGDFEIENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVR EVGTQLLSGAKKVSPGEDIEKVVEAI+EGKLG++L+ CLKN
Sbjct: 661 TYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAIDEGKLGNILMECLKN 711

BLAST of Lcy03g011400 vs. NCBI nr
Match: XP_022987629.1 (phenylalanine ammonia-lyase-like [Cucurbita maxima])

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 681/711 (95.78%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFCSS L + DSVPQ KSFSSVS SD+W KAANALSHTH EEVCRM+SEFQ+ + 
Sbjct: 1   MANSEFFCSSPLYQPDSVPQSKSFSSVSPSDYWNKAANALSHTHLEEVCRMLSEFQDTRA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRS+VKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSNVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEAL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYAL+PKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALMPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK+ENG ETG+AIFKQIPGFLEELK KLE DVETARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGVAIFKQIPGFLEELKEKLENDVETARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG+V++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGNVVLECLKN 711

BLAST of Lcy03g011400 vs. NCBI nr
Match: XP_023516501.1 (phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo] >AXF54191.1 PAL1 [Cucurbita pepo])

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 650/711 (91.42%), Postives = 678/711 (95.36%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFC S L + DSVPQ KSFS VS SD+W KAANALSHTH EEVCRM+SEFQ+A+ 
Sbjct: 1   MANSEFFCYSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARA 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEALKR
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALKR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYALIPKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK ENG ETGIAIFKQIPGFLEELK KLE DVE ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKKENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG V++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of Lcy03g011400 vs. NCBI nr
Match: XP_022961017.1 (phenylalanine ammonia-lyase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 678/711 (95.36%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFCSS L + DSVPQ KSFS VS SD+W KAANALSHTH EEVCRM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEAL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYALIPKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK+ENG ETGIAIFKQIPGFLEELK KLE DVE ARGRFENG+F VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGIAIFKQIPGFLEELKEKLENDVENARGRFENGEFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG +++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSIVLECLKN 711

BLAST of Lcy03g011400 vs. NCBI nr
Match: KAG6590021.1 (Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1272.3 bits (3291), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 676/711 (95.08%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS FFCSS L + DSVPQ KSFS VS SD+W KAANALSHTH EEVCRM+SEFQ+A+ 
Sbjct: 1   MANSEFFCSSPLSQPDSVPQSKSFSFVSPSDYWNKAANALSHTHLEEVCRMLSEFQDARV 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEG TLTVAQVTAIARRSDVKVNLNEAVA DRVAKSA WVAEKIS G D+YGVTTGFG
Sbjct: 61  VDIEGATLTVAQVTAIARRSDVKVNLNEAVAHDRVAKSATWVAEKISSGTDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           +TSHRRTTK ADLQTELIRFLNAGVIGKD+LPS YSKAAMLVRTNTLMQGYSGIRWEILE
Sbjct: 121 STSHRRTTKAADLQTELIRFLNAGVIGKDNLPSGYSKAAMLVRTNTLMQGYSGIRWEILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NL+PKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EGDEITAMEAL R
Sbjct: 181 AVVKLMNENLVPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGDEITAMEALNR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI GPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA
Sbjct: 241 AGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LLNQSDFMKEAKIRHEN+PLTKPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMELLLNQSDFMKEAKIRHENNPLTKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDV+R+LALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVSRNLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDH+NNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHFNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQ EENMREVV+NA+L
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQQEENMREVVRNAVL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+RRTLYV +YGSL ESRFCEKDLLQVI+REPVFSYIDDP NTSYALIPKLLEV+V+RA
Sbjct: 541 QTIRRTLYVTDYGSLTESRFCEKDLLQVIDREPVFSYIDDPTNTSYALIPKLLEVLVQRA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A AK+ENG ETGI IFKQIPGFLEELK KLE DVE ARGRFEN DF VENRIKKCS
Sbjct: 601 LKGIATAKNENGNETGITIFKQIPGFLEELKEKLENDVENARGRFENDDFLVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVV+AINEGKLG V++ CLKN
Sbjct: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVDAINEGKLGSVVLECLKN 711

BLAST of Lcy03g011400 vs. NCBI nr
Match: XP_038879437.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 648/711 (91.14%), Postives = 678/711 (95.36%), Query Frame = 0

Query: 1   MANSGFFCSSTLLETDSVPQPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQT 60
           MANS F CSS L ET+SVPQ KSF SV ASDHWVKAA ALSHTHFEEVCRM+ EFQEAQT
Sbjct: 1   MANSEFLCSSALSETESVPQSKSFPSVLASDHWVKAAKALSHTHFEEVCRMLLEFQEAQT 60

Query: 61  VDIEGTTLTVAQVTAIARRSDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFG 120
           VDIEGTTLTVAQVTAIARRSDVKV LNEAVA DRVA SA WVAEK+S GID+YGVTTGFG
Sbjct: 61  VDIEGTTLTVAQVTAIARRSDVKVILNEAVAHDRVANSAKWVAEKVSKGIDIYGVTTGFG 120

Query: 121 ATSHRRTTKTADLQTELIRFLNAGVIGKDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILE 180
           ATSHRRTTK ADLQTELIRFLNAGVIGKD+LPSSYSKAAMLVRTNTLMQGYSGIRW+ILE
Sbjct: 121 ATSHRRTTKMADLQTELIRFLNAGVIGKDNLPSSYSKAAMLVRTNTLMQGYSGIRWDILE 180

Query: 181 AVVKLMNQNLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKR 240
           AVVKLMN+NLIPKLPLRGTITASGDLVPLSYIAGV+TGRHNSKVTT+EG+ ITAMEALKR
Sbjct: 181 AVVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVITGRHNSKVTTIEGNVITAMEALKR 240

Query: 241 AGIGGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFA 300
           AGI  PFELQAKEGLALVNGTAVGSAVAATVCYDANILALL+V+LSALFCEAMLGKPEFA
Sbjct: 241 AGIAKPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLSVILSALFCEAMLGKPEFA 300

Query: 301 DPLTHKLKHHPGQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLG 360
           DPLTHKLKHHPGQIESAAAME LL QSDFMKEAK R+ENDPL+KPKQDRYALRTSPQWLG
Sbjct: 301 DPLTHKLKHHPGQIESAAAMESLLEQSDFMKEAKTRNENDPLSKPKQDRYALRTSPQWLG 360

Query: 361 PQIEVIRTATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420
           PQIEVIR ATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK
Sbjct: 361 PQIEVIRMATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGK 420

Query: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480
           LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ
Sbjct: 421 LMFAQFSELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQ 480

Query: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALL 540
           SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKN+LL
Sbjct: 481 SAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNSLL 540

Query: 541 QTVRRTLYVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERA 600
           QT+R+TLY+AEYGSLLESRFCEKDLLQVI+RE VFSYIDDP NTSY LIPKLLEV+VE+A
Sbjct: 541 QTIRKTLYIAEYGSLLESRFCEKDLLQVIDREAVFSYIDDPTNTSYTLIPKLLEVLVEKA 600

Query: 601 LKGVAAAKSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCS 660
           LKG+A +K ENG ETGIAIFK+IP FLEELKAKLEKDVE AR RFENG+F VENRIKKCS
Sbjct: 601 LKGIATSKIENGNETGIAIFKKIPEFLEELKAKLEKDVENARERFENGEFEVENRIKKCS 660

Query: 661 TYPIYRFVRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLKN 712
           TYPIYRFVR+EVGTQLLSGAKKVSPGEDIEKV+EAI+EGKLG+V+I CLKN
Sbjct: 661 TYPIYRFVRSEVGTQLLSGAKKVSPGEDIEKVMEAIDEGKLGNVVIECLKN 711

BLAST of Lcy03g011400 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 821.6 bits (2121), Expect = 4.8e-238
Identity = 432/686 (62.97%), Postives = 531/686 (77.41%), Query Frame = 0

Query: 32  HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIAR-RSDVKVNLNEAV 91
           +W  AA+ +  +H +EV +MV E++    V++ G TLT+ QV AI+     VKV L E  
Sbjct: 29  NWGLAADQMKGSHLDEVKKMVEEYRR-PVVNLGGETLTIGQVAAISTVGGSVKVELAE-T 88

Query: 92  ARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGK-- 151
           +R  V  S++WV E ++ G D YGVTTGFGATSHRRT     LQTELIRFLNAG+ G   
Sbjct: 89  SRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNTK 148

Query: 152 ---DSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGD 211
               +LP S ++AAMLVR NTL+QGYSGIR+EILEA+  L+N N+ P LPLRGTITASGD
Sbjct: 149 ETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASGD 208

Query: 212 LVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIG-GPFELQAKEGLALVNGTAVG 271
           LVPLSYIAG++TGR NSK T  +G+ +TA EA ++AGI  G F+LQ KEGLALVNGTAVG
Sbjct: 209 LVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAVG 268

Query: 272 SAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLL 331
           S +A+ V ++AN+ A+LA VLSA+F E M GKPEF D LTH+LKHHPGQIE+AA ME +L
Sbjct: 269 SGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHIL 328

Query: 332 NQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNP 391
           + S +MK A+  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNP
Sbjct: 329 DGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNP 388

Query: 392 LIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSG 451
           LIDV+R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNGLPSNL+ 
Sbjct: 389 LIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTA 448

Query: 452 GPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI 511
             NPSLDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RK++EA+
Sbjct: 449 SSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAV 508

Query: 512 EILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKD 571
           +ILKLMS T++V +CQA+DLR  EEN+R+ VKN + Q  ++ L     G L  SRFCEKD
Sbjct: 509 DILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEKD 568

Query: 572 LLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIP 631
           LL+V++RE VF+Y+DDP + +Y L+ +L +V+V+ AL     +  E  +    +IF++I 
Sbjct: 569 LLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHAL-----SNGETEKNAVTSIFQKIG 628

Query: 632 GFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVS 691
            F EELKA L K+VE AR  + NG   + NRIK+C +YP+YRFVR E+GT+LL+G K VS
Sbjct: 629 AFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVS 688

Query: 692 PGEDIEKVVEAINEGKLGDVLINCLK 711
           PGE+ +KV  A+ EGKL D L++CLK
Sbjct: 689 PGEEFDKVFTAMCEGKLIDPLMDCLK 707

BLAST of Lcy03g011400 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 818.1 bits (2112), Expect = 5.3e-237
Identity = 433/692 (62.57%), Postives = 532/692 (76.88%), Query Frame = 0

Query: 27  VSASD--HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIAR-RSDVK 86
           ++A D  +W  AA  +  +H +EV RMV+EF++   V++ G TLT+ QV AI+   + VK
Sbjct: 30  INAEDPLNWGAAAEQMKGSHLDEVKRMVAEFRK-PVVNLGGETLTIGQVAAISTIGNSVK 89

Query: 87  VNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNA 146
           V L+E  AR  V  S++WV E ++ G D YGVTTGFGATSHRRT     LQ ELIRFLNA
Sbjct: 90  VELSE-TARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNA 149

Query: 147 GVIG-----KDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRG 206
           G+ G       +LP S ++AAMLVR NTL+QG+SGIR+EILEA+   +N N+ P LPLRG
Sbjct: 150 GIFGSTKETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRG 209

Query: 207 TITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGI-GGPFELQAKEGLAL 266
           TITASGDLVPLSYIAG++TGR NSK T   G+ +TA EA K AGI  G F+LQ KEGLAL
Sbjct: 210 TITASGDLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGLAL 269

Query: 267 VNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESA 326
           VNGTAVGS +A+ V ++ N+L++LA +LSA+F E M GKPEF D LTH+LKHHPGQIE+A
Sbjct: 270 VNGTAVGSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAA 329

Query: 327 AAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREI 386
           A ME +L+ S +MK A+  HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREI
Sbjct: 330 AIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIEREI 389

Query: 387 NSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNG 446
           NSVNDNPLIDV+R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV D YNNG
Sbjct: 390 NSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNG 449

Query: 447 LPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISA 506
           LPSNL+   NPSLDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+
Sbjct: 450 LPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISS 509

Query: 507 RKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLE 566
           RK++EA++ILKLMS T++VA+CQA+DLR  EEN+R+ VKN + Q  ++ L     G L  
Sbjct: 510 RKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHP 569

Query: 567 SRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGI 626
           SRFCEKDLL+V++RE V++Y DDP + +Y LI KL +V+V+ AL        E+ +    
Sbjct: 570 SRFCEKDLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHAL-----INGESEKNAVT 629

Query: 627 AIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLL 686
           +IF +I  F EELKA L K+VE AR  ++NG  A+ NRIK+C +YP+YRFVR E+GT+LL
Sbjct: 630 SIFHKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELL 689

Query: 687 SGAKKVSPGEDIEKVVEAINEGKLGDVLINCL 710
           +G K  SPGE+ +KV  AI EGK+ D ++ CL
Sbjct: 690 TGEKVTSPGEEFDKVFTAICEGKIIDPMMECL 714

BLAST of Lcy03g011400 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 817.0 bits (2109), Expect = 1.2e-236
Identity = 430/685 (62.77%), Postives = 519/685 (75.77%), Query Frame = 0

Query: 32  HWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARRSDVKVNLNEAVA 91
           +W   A AL  +H +EV RMV E+++ + V + G TLT+ QV A+AR            A
Sbjct: 19  NWNATAEALKGSHLDEVKRMVKEYRK-EAVKLGGETLTIGQVAAVARGGGGSTVELAEEA 78

Query: 92  RDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIG---- 151
           R  V  S+ WV E ++ G D YGVTTGFGATSHRRT +   LQ ELIRFLNAG+ G    
Sbjct: 79  RAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPGAG 138

Query: 152 --KDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLIPKLPLRGTITASGD 211
               +LP   ++AAMLVR NTL+QGYSGIR+EILEA+ KL+N  + P LPLRGTITASGD
Sbjct: 139 DTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITASGD 198

Query: 212 LVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIGGPFELQAKEGLALVNGTAVGS 271
           LVPLSYIAG++TGR NSK     G+ +TA EA K AG+   FELQ KEGLALVNGTAVGS
Sbjct: 199 LVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGVSSFFELQPKEGLALVNGTAVGS 258

Query: 272 AVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHPGQIESAAAMEFLLN 331
            +A+TV +DANILA+L+ V+SA+F E M GKPEF D LTHKLKHHPGQIE+AA ME +L+
Sbjct: 259 GLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILD 318

Query: 332 QSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATHSIEREINSVNDNPL 391
            S ++KEA++ HE DPL KPKQDRYALRTSPQWLGPQIEVIR AT  IEREINSVNDNPL
Sbjct: 319 GSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDNPL 378

Query: 392 IDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDHYNNGLPSNLSGG 451
           IDV+R+ ALHGGNFQGTPIGV+MDN R+AIA+IGKLMFAQFSELV D YNNGLPSNLSGG
Sbjct: 379 IDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGG 438

Query: 452 PNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIE 511
            NPSLDYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA++
Sbjct: 439 RNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVD 498

Query: 512 ILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAEYGSLLESRFCEKDL 571
           ILKLMS TY+VALCQA+DLR  EEN+++ VK+A+ Q  +R L V   G L  SRF E+D+
Sbjct: 499 ILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTERDV 558

Query: 572 LQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSENGEETGIAIFKQIPG 631
           LQV++RE VFSY DDP + +Y L+ KL  ++V+ AL     A  E    +  ++F +I  
Sbjct: 559 LQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHAL-----ADPEREANSATSVFHKIGA 618

Query: 632 FLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTEVGTQLLSGAKKVSP 691
           F  ELK  L K+VE  R  +E G  A+ NRIK+C +YP+YRFVR E+ T+LL+G    SP
Sbjct: 619 FEAELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSP 678

Query: 692 GEDIEKVVEAINEGKLGDVLINCLK 711
           GE+ +KV  AI++GKL D L+ CLK
Sbjct: 679 GEEFDKVFLAISDGKLIDPLLECLK 697

BLAST of Lcy03g011400 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 751.1 bits (1938), Expect = 8.0e-217
Identity = 408/699 (58.37%), Postives = 509/699 (72.82%), Query Frame = 0

Query: 20  QPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARR 79
           QP + +++S   +W  AA AL  +H EEV +MV ++++  TV + G TLT+ QV A+A  
Sbjct: 5   QPNA-TALSDPLNWNVAAEALKGSHLEEVKKMVKDYRKG-TVQLGGETLTIGQVAAVASG 64

Query: 80  SDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIR 139
               V L+E  AR  V  S++WV E ++   D YG+TTGFG++S RRT + A LQ ELIR
Sbjct: 65  GPT-VELSEE-ARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIR 124

Query: 140 FLNAGVIG--------KDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLI 199
           +LNAG+           ++LP   ++AAML+R NTL+QGYSGIR+EILEA+  L+N  + 
Sbjct: 125 YLNAGIFATGNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKIT 184

Query: 200 PKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIGGPFELQA 259
           P LPLRGTITASGDLVPLSYIAG + GR NS+     G+ +TA+EA K AG+   FEL+ 
Sbjct: 185 PLLPLRGTITASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSSFFELRP 244

Query: 260 KEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHP 319
           KEGLALVNGTAVGSA+A+TV YDANIL + + V SA+F E M GKPEF D LTHKLKHHP
Sbjct: 245 KEGLALVNGTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHP 304

Query: 320 GQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDRYALRTSPQWLGPQIEVIRTATH 379
           GQIE+AA ME +L+ S ++KEA   H+ DPL KPKQDRYALRTSPQWLGPQIEVIR AT 
Sbjct: 305 GQIEAAAIMEHILDGSSYVKEALHLHKIDPLQKPKQDRYALRTSPQWLGPQIEVIRAATK 364

Query: 380 SIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVC 439
            IEREINSVNDNPLIDV+R+ A+HGGNFQGTPIGV+MDN R+A+A+IGKLMFAQF+ELV 
Sbjct: 365 MIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVN 424

Query: 440 DHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNS 499
           D YNNGLPSNLSGG NPSLDYG KGAE+AMA+YCSELQ+LANPVT HV+SA QHNQDVNS
Sbjct: 425 DFYNNGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNS 484

Query: 500 LGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTLYVAE 559
           LGLIS+R +AEA+ ILKLMS TY+VALCQA DLR  EE +++ V   +  T +  L +  
Sbjct: 485 LGLISSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVLAIEP 544

Query: 560 YGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAAKSEN 619
           +           D+L V+ RE VFSY+DDP++ +  L+ KL  V+ ++AL     A+ E 
Sbjct: 545 FRK-------HDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKAL-----AEPEG 604

Query: 620 GEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRFVRTE 679
             +T   +F++I  F  ELK  L K+VE  R  +ENG F V NRIKKC +YP+YRFVR E
Sbjct: 605 ETDT---VFRKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNE 664

Query: 680 VGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLK 711
           + T+LL+G    SPGED +KV  AI++GKL D L  CLK
Sbjct: 665 LETRLLTGEDVRSPGEDFDKVFRAISQGKLIDPLFECLK 684

BLAST of Lcy03g011400 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 745.3 bits (1923), Expect = 4.4e-215
Identity = 408/703 (58.04%), Postives = 509/703 (72.40%), Query Frame = 0

Query: 20  QPKSFSSVSASDHWVKAANALSHTHFEEVCRMVSEFQEAQTVDIEGTTLTVAQVTAIARR 79
           QP + +++S   +W  AA AL  +H EEV +MV ++++  TV + G TLT+ QV A+A  
Sbjct: 5   QPNA-TALSDPLNWNVAAEALKGSHLEEVKKMVKDYRKG-TVQLGGETLTIGQVAAVASG 64

Query: 80  SDVKVNLNEAVARDRVAKSANWVAEKISCGIDVYGVTTGFGATSHRRTTKTADLQTELIR 139
               V L+E  AR  V  S++WV E ++   D YG+TTGFG++S RRT + A LQ ELIR
Sbjct: 65  GPT-VELSEE-ARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIR 124

Query: 140 FLNAGVIG--------KDSLPSSYSKAAMLVRTNTLMQGYSGIRWEILEAVVKLMNQNLI 199
           +LNAG+           ++LP   ++AAML+R NTL+QGYSGIR+EILEA+  L+N  + 
Sbjct: 125 YLNAGIFATGNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKIT 184

Query: 200 PKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTMEGDEITAMEALKRAGIGGPFELQA 259
           P LPLRGTITASGDLVPLSYIAG + GR NS+     G+ +TA+EA K AG+   FEL+ 
Sbjct: 185 PLLPLRGTITASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSSFFELRP 244

Query: 260 KEGLALVNGTAVGSAVAATVCYDANILALLAVVLSALFCEAMLGKPEFADPLTHKLKHHP 319
           KEGLALVNGTAVGSA+A+TV YDANIL + + V SA+F E M GKPEF D LTHKLKHHP
Sbjct: 245 KEGLALVNGTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHP 304

Query: 320 GQIESAAAMEFLLNQSDFMKEAKIRHENDPLTKPKQDR----YALRTSPQWLGPQIEVIR 379
           GQIE+AA ME +L+ S ++KEA   H+ DPL KPKQDR    YALRTSPQWLGPQIEVIR
Sbjct: 305 GQIEAAAIMEHILDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIR 364

Query: 380 TATHSIEREINSVNDNPLIDVNRDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFS 439
            AT  IEREINSVNDNPLIDV+R+ A+HGGNFQGTPIGV+MDN R+A+A+IGKLMFAQF+
Sbjct: 365 AATKMIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFT 424

Query: 440 ELVCDHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQ 499
           ELV D YNNGLPSNLSGG NPSLDYG KGAE+AMA+YCSELQ+LANPVT HV+SA QHNQ
Sbjct: 425 ELVNDFYNNGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQ 484

Query: 500 DVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQYEENMREVVKNALLQTVRRTL 559
           DVNSLGLIS+R +AEA+ ILKLMS TY+VALCQA DLR  EE +++ V   +  T +  L
Sbjct: 485 DVNSLGLISSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL 544

Query: 560 YVAEYGSLLESRFCEKDLLQVIEREPVFSYIDDPANTSYALIPKLLEVVVERALKGVAAA 619
            +  +           D+L V+ RE VFSY+DDP++ +  L+ KL  V+ ++AL     A
Sbjct: 545 AIEPFRK-------HDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKAL-----A 604

Query: 620 KSENGEETGIAIFKQIPGFLEELKAKLEKDVETARGRFENGDFAVENRIKKCSTYPIYRF 679
           + E   +T   +F++I  F  ELK  L K+VE  R  +ENG F V NRIKKC +YP+YRF
Sbjct: 605 EPEGETDT---VFRKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRF 664

Query: 680 VRTEVGTQLLSGAKKVSPGEDIEKVVEAINEGKLGDVLINCLK 711
           VR E+ T+LL+G    SPGED +KV  AI++GKL D L  CLK
Sbjct: 665 VRNELETRLLTGEDVRSPGEDFDKVFRAISQGKLIDPLFECLK 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527772.9e-24864.24Phenylalanine ammonia-lyase OS=Pinus taeda OX=3352 GN=PAL PE=3 SV=1[more]
A0A2H5AIW01.0e-24064.29Phenylalanine ammonia-lyase 1 OS=Narcissus pseudonarcissus OX=39639 GN=PAL1 PE=2... [more]
Q50EX72.2e-24064.63Phenylalanine ammonia-lyase 1 OS=Petunia hybrida OX=4102 GN=PAL1 PE=1 SV=1[more]
P457263.2e-23963.70Phenylalanine ammonia-lyase OS=Camellia sinensis OX=4442 GN=PAL PE=2 SV=1[more]
O238656.1e-23863.56Phenylalanine ammonia-lyase 1 OS=Daucus carota OX=4039 GN=PAL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JJE50.0e+0091.14Phenylalanine ammonia-lyase OS=Cucurbita maxima OX=3661 GN=LOC111485128 PE=3 SV=... [more]
A0A345BTD40.0e+0091.42Phenylalanine ammonia-lyase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1H8Z90.0e+0091.14Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111461649 PE=3 S... [more]
A0A6J1D3950.0e+0090.58Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111016602 PE=3 ... [more]
A0A0A0LYZ40.0e+0089.87Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_1G590300 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022987629.10.0e+0091.14phenylalanine ammonia-lyase-like [Cucurbita maxima][more]
XP_023516501.10.0e+0091.42phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo] >AXF54191.1 PAL1 [... [more]
XP_022961017.10.0e+0091.14phenylalanine ammonia-lyase-like isoform X1 [Cucurbita moschata][more]
KAG6590021.10.0e+0091.14Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038879437.10.0e+0091.14phenylalanine ammonia-lyase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G53260.14.8e-23862.97phenylalanine ammonia-lyase 2 [more]
AT2G37040.15.3e-23762.57PHE ammonia lyase 1 [more]
AT3G10340.11.2e-23662.77phenylalanine ammonia-lyase 4 [more]
AT5G04230.18.0e-21758.37phenyl alanine ammonia-lyase 3 [more]
AT5G04230.24.4e-21558.04phenyl alanine ammonia-lyase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005922Phenylalanine ammonia-lyaseTIGRFAMTIGR01226TIGR01226coord: 31..710
e-value: 1.4E-301
score: 999.8
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 22..261
e-value: 3.6E-81
score: 274.2
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 68..544
e-value: 9.4E-154
score: 512.5
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 35..710
IPR001106Aromatic amino acid lyaseCDDcd00332PAL-HALcoord: 66..525
e-value: 0.0
score: 540.565
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 262..710
e-value: 1.3E-214
score: 715.1
NoneNo IPR availablePANTHERPTHR10362:SF58PHENYLALANINE AMMONIA-LYASEcoord: 35..710
IPR023144Phenylalanine ammonia-lyase, shielding domain superfamilyGENE3D1.10.274.20coord: 528..654
e-value: 1.3E-214
score: 715.1
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 198..214
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 32..710

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy03g011400.1Lcy03g011400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity