Lcy03g002490 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy03g002490
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr03: 8785968 .. 8786423 (+)
RNA-Seq Expression	Lcy03g002490
Synteny	Lcy03g002490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTGTTCGGCACTGGAATGGTACCCCCCTTTTCGCCGGATTTCGTTGCATCTCAAGACAATGGAGGATTCCTTGGCTGGAGAGACTATGGCGATTCGAGAAGGCCTTCGTGCGCTGTCGTCGGACTCTCTTGCGGTTATGGTGGAGAGTGATGCCCTTCAAGTTATCAGACTGCTAACCGGTAAAGAAGACGATGAAACTGAGTTGAAGTTCTTCATTGGGGAAGTAGAATCCCTAAAAGTGGAAAGGCATGTGGATGGTTTCTATCATGTTTTGAAGAGGCACAATCAAATGGCTCATTATTTGGCTAGAAAGGCATGTCTGAGTGGGATTTCTGCATGTTGGGTCCATTCATTTCCTAATTGGCTACTTGATTTAAATTTTGTTGACATTGGAGGTTATGATACCACAAGTGGGGGATCCTGTCCCACTGGCGGTATCCCTTTGGCTTAG

mRNA sequence

ATGGATTGTTCGGCACTGGAATGGTACCCCCCTTTTCGCCGGATTTCGTTGCATCTCAAGACAATGGAGGATTCCTTGGCTGGAGAGACTATGGCGATTCGAGAAGGCCTTCGTGCGCTGTCGTCGGACTCTCTTGCGGTTATGGTGGAGAGTGATGCCCTTCAAGTTATCAGACTGCTAACCGGTAAAGAAGACGATGAAACTGAGTTGAAGTTCTTCATTGGGGAAGTAGAATCCCTAAAAGTGGAAAGGCATGTGGATGGTTTCTATCATGTTTTGAAGAGGCACAATCAAATGGCTCATTATTTGGCTAGAAAGGCATGTCTGAGTGGGATTTCTGCATGTTGGGTCCATTCATTTCCTAATTGGCTACTTGATTTAAATTTTGTTGACATTGGAGGTTATGATACCACAAGTGGGGGATCCTGTCCCACTGGCGGTATCCCTTTGGCTTAG

Coding sequence (CDS)

ATGGATTGTTCGGCACTGGAATGGTACCCCCCTTTTCGCCGGATTTCGTTGCATCTCAAGACAATGGAGGATTCCTTGGCTGGAGAGACTATGGCGATTCGAGAAGGCCTTCGTGCGCTGTCGTCGGACTCTCTTGCGGTTATGGTGGAGAGTGATGCCCTTCAAGTTATCAGACTGCTAACCGGTAAAGAAGACGATGAAACTGAGTTGAAGTTCTTCATTGGGGAAGTAGAATCCCTAAAAGTGGAAAGGCATGTGGATGGTTTCTATCATGTTTTGAAGAGGCACAATCAAATGGCTCATTATTTGGCTAGAAAGGCATGTCTGAGTGGGATTTCTGCATGTTGGGTCCATTCATTTCCTAATTGGCTACTTGATTTAAATTTTGTTGACATTGGAGGTTATGATACCACAAGTGGGGGATCCTGTCCCACTGGCGGTATCCCTTTGGCTTAG

Protein sequence

MDCSALEWYPPFRRISLHLKTMEDSLAGETMAIREGLRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGEVESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDLNFVDIGGYDTTSGGSCPTGGIPLA

Homology

[Close]

BLAST of Lcy03g002490 vs. ExPASy TrEMBL
Match: A0A7H4LJB8 (Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCUS3319 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.2e-05
Identity = 37/110 (33.64%), Postives = 59/110 (53.64%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREGLR-ALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           HL + +D L  E +AIREGL  AL   SL + +ESD+L+ + ++  +  + ++  FFI E
Sbjct: 17  HLFSCDDILEAEILAIREGLMLALQWSSLPIYIESDSLEAVNMVKSENTNRSKYAFFIRE 76

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLD 127
           ++    ER      H+ +  N  +H LA      G  A W+ S P+ +LD
Sbjct: 77  IKDSMSERS-SCITHIHRSCNNSSHILANFERTQGRIAVWLGSRPDAVLD 125

[Close]

BLAST of Lcy03g002490 vs. ExPASy TrEMBL
Match: A2YJD5 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_25347 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.6e-05
Identity = 32/104 (30.77%), Postives = 55/104 (52.88%), Query Frame = 0


Query: 25  SLAGETMAIREGLR-ALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGEVESLKVE 84
           +L  E +A R+GL   L    L +++ESD LQ I+L+  KE++ ++L F I E++ L   
Sbjct: 751 ALEAELLACRDGLTLVLQWTLLPIVIESDCLQAIQLIQSKEEERSDLAFLIREIKHLMAG 810

Query: 85  RHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDL 128
                   + +  N ++H LA K C   ++A W+    N++  L
Sbjct: 811 NRDIVIMKIHRNQNSVSHALAYKGCSEALTAFWLDDTCNFIAHL 854

[Close]

BLAST of Lcy03g002490 vs. ExPASy TrEMBL
Match: A0A314ZLL7 (RNase H domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_14707 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 6.1e-05
Identity = 36/113 (31.86%), Postives = 59/113 (52.21%), Query Frame = 0


Query: 16  SLHLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFI 75
           +LH+  +  +L  E MA REG + A+  D   V+ ESD+LQ++  +     D + +   +
Sbjct: 150 ALHVDNVFSALQVEAMAAREGAILAVEGDFNNVIFESDSLQIVSAIRSPSVDRSFVGPVV 209

Query: 76  GEVESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDL 128
            + ++L  +   DGF H+ +  N  AH LAR A   G  A W    P+ L+D+
Sbjct: 210 EDTKALLTQITGDGFTHIRRVANGAAHRLARYASHIGTMATWFEEPPDLLVDI 262

[Close]

BLAST of Lcy03g002490 vs. ExPASy TrEMBL
Match: B9F3B9 (RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_05582 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.9e-04
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           +++    +L  E MA REG L AL    L +++E+D  +++ LL  KE   +EL F + E
Sbjct: 147 YIQRCTGALEAELMACREGLLMALQWTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKE 206

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDLNFVD 132
           V  L +   V     V +  N+++H LA K  +  ++  W  +  N +  L  VD
Sbjct: 207 VHDLMMGNRVVSLAKVHRNQNRISHVLANKGRVEELTHFWPDNSCNAIAQLVCVD 261

[Close]

BLAST of Lcy03g002490 vs. ExPASy TrEMBL
Match: A0A0P0VFG5 (Os02g0175302 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0175302 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.9e-04
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           +++    +L  E MA REG L AL    L +++E+D  +++ LL  KE   +EL F + E
Sbjct: 36  YIQRCTGALEAELMACREGLLMALQWTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKE 95

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDLNFVD 132
           V  L +   V     V +  N+++H LA K  +  ++  W  +  N +  L  VD
Sbjct: 96  VHDLMMGNRVVSLAKVHRNQNRISHVLANKGRVEELTHFWPDNSCNAIAQLVCVD 150

[Close]

BLAST of Lcy03g002490 vs. NCBI nr
Match: SPT18706.1 (unnamed protein product [Triticum aestivum])

HSP 1 Score: 59.7 bits (143), Expect = 2.5e-05
Identity = 37/110 (33.64%), Postives = 59/110 (53.64%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREGLR-ALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           HL + +D L  E +AIREGL  AL   SL + +ESD+L+ + ++  +  + ++  FFI E
Sbjct: 17  HLFSCDDILEAEILAIREGLMLALQWSSLPIYIESDSLEAVNMVKSENTNRSKYAFFIRE 76

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLD 127
           ++    ER      H+ +  N  +H LA      G  A W+ S P+ +LD
Sbjct: 77  IKDSMSERS-SCITHIHRSCNNSSHILANFERTQGRIAVWLGSRPDAVLD 125

[Close]

BLAST of Lcy03g002490 vs. NCBI nr
Match: EAZ03196.1 (hypothetical protein OsI_25347 [Oryza sativa Indica Group])

HSP 1 Score: 58.2 bits (139), Expect = 7.3e-05
Identity = 32/104 (30.77%), Postives = 55/104 (52.88%), Query Frame = 0


Query: 25  SLAGETMAIREGLR-ALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGEVESLKVE 84
           +L  E +A R+GL   L    L +++ESD LQ I+L+  KE++ ++L F I E++ L   
Sbjct: 751 ALEAELLACRDGLTLVLQWTLLPIVIESDCLQAIQLIQSKEEERSDLAFLIREIKHLMAG 810

Query: 85  RHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDL 128
                   + +  N ++H LA K C   ++A W+    N++  L
Sbjct: 811 NRDIVIMKIHRNQNSVSHALAYKGCSEALTAFWLDDTCNFIAHL 854

[Close]

BLAST of Lcy03g002490 vs. NCBI nr
Match: PQQ17701.1 (hypothetical protein Pyn_14707 [Prunus yedoensis var. nudiflora])

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-04
Identity = 36/113 (31.86%), Postives = 59/113 (52.21%), Query Frame = 0


Query: 16  SLHLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFI 75
           +LH+  +  +L  E MA REG + A+  D   V+ ESD+LQ++  +     D + +   +
Sbjct: 150 ALHVDNVFSALQVEAMAAREGAILAVEGDFNNVIFESDSLQIVSAIRSPSVDRSFVGPVV 209

Query: 76  GEVESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDL 128
            + ++L  +   DGF H+ +  N  AH LAR A   G  A W    P+ L+D+
Sbjct: 210 EDTKALLTQITGDGFTHIRRVANGAAHRLARYASHIGTMATWFEEPPDLLVDI 262

[Close]

BLAST of Lcy03g002490 vs. NCBI nr
Match: BAS77245.1 (Os02g0175302 [Oryza sativa Japonica Group])

HSP 1 Score: 54.7 bits (130), Expect = 8.1e-04
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           +++    +L  E MA REG L AL    L +++E+D  +++ LL  KE   +EL F + E
Sbjct: 36  YIQRCTGALEAELMACREGLLMALQWTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKE 95

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDLNFVD 132
           V  L +   V     V +  N+++H LA K  +  ++  W  +  N +  L  VD
Sbjct: 96  VHDLMMGNRVVSLAKVHRNQNRISHVLANKGRVEELTHFWPDNSCNAIAQLVCVD 150

[Close]

BLAST of Lcy03g002490 vs. NCBI nr
Match: EEE56417.1 (hypothetical protein OsJ_05582 [Oryza sativa Japonica Group])

HSP 1 Score: 54.7 bits (130), Expect = 8.1e-04
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 18  HLKTMEDSLAGETMAIREG-LRALSSDSLAVMVESDALQVIRLLTGKEDDETELKFFIGE 77
           +++    +L  E MA REG L AL    L +++E+D  +++ LL  KE   +EL F + E
Sbjct: 147 YIQRCTGALEAELMACREGLLMALQWTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKE 206

Query: 78  VESLKVERHVDGFYHVLKRHNQMAHYLARKACLSGISACWVHSFPNWLLDLNFVD 132
           V  L +   V     V +  N+++H LA K  +  ++  W  +  N +  L  VD
Sbjct: 207 VHDLMMGNRVVSLAKVHRNQNRISHVLANKGRVEELTHFWPDNSCNAIAQLVCVD 261

The following BLAST results are available for this feature:

BLAST of Lcy03g002490 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy03g002490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7H4LJB8	1.2e-05	33.64	Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCU...	[more]
A2YJD5	3.6e-05	30.77	Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. indica OX...	[more]
A0A314ZLL7	6.1e-05	31.86	RNase H domain-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 ...	[more]
B9F3B9	3.9e-04	30.43	RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os...	[more]
A0A0P0VFG5	3.9e-04	30.43	Os02g0175302 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0175302 PE...	[more]

BLAST of Lcy03g002490 vs. NCBI nr


Match Name	E-value	Identity	Description
SPT18706.1	2.5e-05	33.64	unnamed protein product [Triticum aestivum]	[more]
EAZ03196.1	7.3e-05	30.77	hypothetical protein OsI_25347 [Oryza sativa Indica Group]	[more]
PQQ17701.1	1.3e-04	31.86	hypothetical protein Pyn_14707 [Prunus yedoensis var. nudiflora]	[more]
BAS77245.1	8.1e-04	30.43	Os02g0175302 [Oryza sativa Japonica Group]	[more]
EEE56417.1	8.1e-04	30.43	hypothetical protein OsJ_05582 [Oryza sativa Japonica Group]	[more]

BLAST of Lcy03g002490 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 11..112 e-value: 1.2E-5 score: 27.3
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 19..107 e-value: 8.0E-10 score: 38.6
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 24..105 e-value: 2.93606E-9 score: 50.0052
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 19..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy03g002490.1	Lcy03g002490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity