Lcy02g012520 (gene) Sponge gourd (P93075) v1

Overview
NameLcy02g012520
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAlpha/beta hydrolase-1
LocationChr02: 37666649 .. 37670825 (+)
RNA-Seq ExpressionLcy02g012520
SyntenyLcy02g012520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAATAGCTTAGTCTATAATTATATATCCAATATTTGGCTTGCCCTCTTGTTTTCTCACCCAAAGGGAAGTTCAACCACCACCCACCCTCTTTTTCCTTTGCCCCAACAGATGGCGTTAAATGAAAACAACATTCAATTCATTACTTCATCTACTATCTGCTACTCTCTCTGACTCTTCATTTCTCTCCACAAACCCAAACCAATGAAGAAGAAGCAACAATTACAACAACACACAAAGCTAACCCCTTTTTAACCCCTCTTTCTAAAACCTTCGTCCCTTCTCCCATTATCTTCAATGGCCACCAAATCATCAGCCAAATGGGTCTTCATCACGCTCGCCCGTTTCTCCAACGAAGCCCTCAGCTTCTTCCTCTTCTCTCTTCTCGACCTCCTCGACATTCTCCTCTGCTTTCTCTACAAACTCGCTGATTTCTTCTTTGAATCCGAGTGGAAGCCTTGCTATTGCTCCTCTCATCAGGAGTCTTTTGCCAGTAGTAAGATTTTGGTCTCCCAGAAGGCTCTCTCTTCCTCCACTAAGCTTCATCTCGAGCAACTTTCTGATACTCTCTACACTCGTCCTTCTCTCCTCTCCGAGCTCTCCAAATTCACTGTCAATGAGCTCCGCCGCCTCAAGGTTAAGCCTTTTCTTCTTCGCTCCTCCGCCGCGGCCCCGGTCGGTTCCACGTTTTCGGTTAACTCTACCATTGTTGAGATGCTTCAGGAGAAGATCAATGGCGGACAGAGTCCTAGGTGGTCTGAGTGTAATTGTAAACTTTGTACTTGTTGGTCTTCTCCCTCTAAACAATCGCTCTTCGTTCGTTCTCAAGGACCTAGAGGTAATTTGATTCTATTTTAGTCTCGAAACTTTTGAGATGATTTGACTTTTGATGGATTGGTTTTAATATTTTGTTTGTAAACTTTTAAAATGTTTATTTTTTATATTGTACTTTTTTAAAAAAAATAAAAAGTGAACTTTTGAAATGTCTGTTTTAAATATGGATACTTTTGAAAATGGATTATTTTGGTCATTATTGTCACCTAATGTTTGCTAATACTTACCTACTTATCACCATTAAAAATTTGACATCCATTTACTTTTCTAGGTAAAAAGAAAAAAAATCTGTCTTCCATCTTTTTCTTTTTTGCATTCTTCTCTCGTGGTAAAATTCATAATAGACCATAAATTTGGTGGTCTATTTGTAAAAGGAAAAAACTTGGCTACATACCAGTGTACAAGGATCTCCTTACATATCATCGTTAAAGTCTTGACCACCAAGAGAGATAAAAAAAGTTTTCTCATTTTCTCCATTCCTCTTTTTTATTTGGGAAGATTTTAATAGAGTGTGAAGTTTGCAGCTAAGTTTTGTCTTCTGTTTGAGAGTTAAACCCAAAAAAAAATGACAAAAATGAACCTTTTGGAAAGTAGAGACCAAATTTTTTTCAACAAAAATCTCAAATTTTCAAGTACCAAAAGAGAAAAAATTAAAAGCCAAGTAACCACTTAAACCTAATTGATCAATGAATGCACGTACAACTGTAGAAACATAACTCTCAAGAACTGCTGGTTTTCAGATCAGCCACGTGAAGACGTACTGTTCATTCATGGCTTCATTTCTTCATCCGCGTTCTGGACGGAAACCCTCTTCCCAAACTTCTCGGCGTCCGCCAAATCGACATATCGGCTCCTCGCTGTCGATCTGCTCGGATTCGGTCGGAGCCCGAAGCCAGCTGATTCACTCTACACATTGAAGGAGCACGTCGACATGATCGAAACCTCAGTTCTTGAAGCCTACAAGGTCAAGTCCTTCCACATCGTCGCTCATTCCCTTGGCTGCATTTTGGCCCTGGCTTTAGCCGTCAAGCATCCCGGCTCAGTCAAGTCACTAACATTACTTGCACCGGTATGTTCAAATTCCTGTAATATCATTTTGTTTTTTCTTTAACTAGTACTTTGTTCGGTCATCTTGGAATAGACACTTATTGACGGTTGGGTAGACATTTCTTCCGGAGAATGAAATGTTTCTTTAATTTAAAAATTTAAGCTTTTGAGTTAAGTGGTGATTTAACGGTTAATAAATGGATCACAGCCTTATTATCCCGTGCCAAAAGGGGAAGAGGCGAGCCAATACGTGATGAGGAAGGTGGCGCCGCGGCGGGTGTGGCCGCCGATAGCGCTGGGGGCGTCGATTGCTTGCTGGTACGAGCACATCTCAAGGACGGTGTGCCTACTCATATGCAAGAACCATCGCTTTTGGGAGTTTCTAACCAAACTTGTCACAAGAAACAGGTATTTTGTTCTCTTTTTTCTTACCAAACTTCAAAATCTTATGTTTTTCTTCTCCTTTTTAGGAAATAAAAGCCAACCACAATTGGAATCTTAAAAAAAAATAGTTAAACGCCTTTTGAGAATGGTTTTGATTTTGATTTCAATTTGATTTTTGGTTTTTGATTTATAGTTTTAGTTATGCGCTCGAGTGTTGTTAATCTTTGAAATTATGGAATAGATTCCTGATCTTCCATTTTTGTATTTAATGTCTCTGACAGGTTTGACATTTTTCAAAAGTCATAGATTTGTTAAACATAAAATTCAATTTTGTGTCTAATGGAACATTTGACCTCTCATATTTTGTGTCAAGAGGAACTAATAAATAGAAAATTGAAAATTTTTGAGCTCTTGAACACTTTTCAAATTAGGTTTGTAATTTAATCAATTTTGATATTTGAAAATTAGGCTGGTTTTTTAGATTACATTTTTGTAAGTAAATTATCAAGGGAAGAAAATTATTAGTAAAAACCCCTCAAATTGGTTTTGTTTGAATTTTCAAGAACTGTTTTTAAGAATGGTGAACCATTGCAAGACGTATGGTCCATGTAGCTCACAATTTATAAAAGTTGGTTGATTTGATATTTTCTCTTTCTATATCACTTTCCATCGTACTATTAATGAAATAAATAGAATAGACATCACTAATTTTTTTTATTTTCGTATAAAAAAATTAAAGAATAAAAAACAAAATTGTAAAAACCTACAATATTTCTCATCCTATCCGGTCCAAGTCAACCAAATTTATGACTCTAGTCAGGTTCAAAGCGATCAACATTATGACCCTGGCCCTTAAGAACAGTTTGATGTTCTATCTCCCTTTCAATCTTTTGAGCGAAATGTATGAAAAATCTAACTTGTCAGTAAGTACCCTTTCTCTTGGGTCCGAGTTGTTGAATTTAGTTGGTTTGGACAGGGGTGGGTCAATTTTTTTTTTTTATTAATTTCCAATCTTGACTCCAAAAAATAAAATTAAAAATTTAACGCGCTTATTTATTATATCTATTGTCATGTACGTTAGAGGCATGTAGGAAAAAAGACCACAAAACTGACACAAAATAAGCTAGTGGATGTATTTTATGAGATATTTACTCTTGGTAATTTAATGCTAGCTACTAACACCTTCTACTTCTATAGTCTATCATATTATTTATTTTTCCAATTAGAAACATTATTTTAAAATTATTTACATTTAATGGAAGGAAAATTACTTTAAAATGGTATTAAGTTTTATTAAATTTATTTATTTCCAAAATTGGATATTGGATGAAAAACTAAAAAAGAAATGCAATTGATTTTTTTTTTTTAATTTTTGCAGGATAGAGTCATTTTTGGTGGAGGGATTCTTCGCCCACACGCACAACGCCGCGTGGCATACACTGCACAACGTGATATGCGGTACCGGCGGGAAGATCGAAAGGTATTTGGACGCAATCCGAGAGCGCGTGAACTGCCGCGTGAACATATTCCACGGCGGCGACGACGACGTGATTCCGTTGGAGTGCAGCTACAACGTGAAGGCGCGTGTGCCGAGGGCGCGTGTGAATGTGGTGCAAAACAAAGACCACATCACAATCGTCGTGGGAAGACAAAAAGCCTTTGCCAGAGAGCTTGAGCAAATTTGGAGTAACTCAAATGCTTCAATTTAAAATAAATTCAAGCAAATATTGTTATTCTCATTATCAATATATCTTAATCGTAAATTAAAAGTTTTATATAAAAAAAAAATCTTATTCTCAAGAAAAAGATTAAAGATTTAAATAACCTGAAAAGCAGGTTTTTTTTTTTTTTTTTTTTGTGATCTTCTTCATGTGTGAAAAGTCTGTATTTTGTGGGGGCAAAGCCGTG

mRNA sequence

AAAAAAGAATAGCTTAGTCTATAATTATATATCCAATATTTGGCTTGCCCTCTTGTTTTCTCACCCAAAGGGAAGTTCAACCACCACCCACCCTCTTTTTCCTTTGCCCCAACAGATGGCGTTAAATGAAAACAACATTCAATTCATTACTTCATCTACTATCTGCTACTCTCTCTGACTCTTCATTTCTCTCCACAAACCCAAACCAATGAAGAAGAAGCAACAATTACAACAACACACAAAGCTAACCCCTTTTTAACCCCTCTTTCTAAAACCTTCGTCCCTTCTCCCATTATCTTCAATGGCCACCAAATCATCAGCCAAATGGGTCTTCATCACGCTCGCCCGTTTCTCCAACGAAGCCCTCAGCTTCTTCCTCTTCTCTCTTCTCGACCTCCTCGACATTCTCCTCTGCTTTCTCTACAAACTCGCTGATTTCTTCTTTGAATCCGAGTGGAAGCCTTGCTATTGCTCCTCTCATCAGGAGTCTTTTGCCAGTAGTAAGATTTTGGTCTCCCAGAAGGCTCTCTCTTCCTCCACTAAGCTTCATCTCGAGCAACTTTCTGATACTCTCTACACTCGTCCTTCTCTCCTCTCCGAGCTCTCCAAATTCACTGTCAATGAGCTCCGCCGCCTCAAGGTTAAGCCTTTTCTTCTTCGCTCCTCCGCCGCGGCCCCGGTCGGTTCCACGTTTTCGGTTAACTCTACCATTGTTGAGATGCTTCAGGAGAAGATCAATGGCGGACAGAGTCCTAGGTGGTCTGAGTGTAATTGTAAACTTTGTACTTGTTGGTCTTCTCCCTCTAAACAATCGCTCTTCGTTCGTTCTCAAGGACCTAGAGATCAGCCACGTGAAGACGTACTGTTCATTCATGGCTTCATTTCTTCATCCGCGTTCTGGACGGAAACCCTCTTCCCAAACTTCTCGGCGTCCGCCAAATCGACATATCGGCTCCTCGCTGTCGATCTGCTCGGATTCGGTCGGAGCCCGAAGCCAGCTGATTCACTCTACACATTGAAGGAGCACGTCGACATGATCGAAACCTCAGTTCTTGAAGCCTACAAGGTCAAGTCCTTCCACATCGTCGCTCATTCCCTTGGCTGCATTTTGGCCCTGGCTTTAGCCGTCAAGCATCCCGGCTCAGTCAAGTCACTAACATTACTTGCACCGCCTTATTATCCCGTGCCAAAAGGGGAAGAGGCGAGCCAATACGTGATGAGGAAGGTGGCGCCGCGGCGGGTGTGGCCGCCGATAGCGCTGGGGGCGTCGATTGCTTGCTGGTACGAGCACATCTCAAGGACGGTGTGCCTACTCATATGCAAGAACCATCGCTTTTGGGAGTTTCTAACCAAACTTGTCACAAGAAACAGGATAGAGTCATTTTTGGTGGAGGGATTCTTCGCCCACACGCACAACGCCGCGTGGCATACACTGCACAACGTGATATGCGGTACCGGCGGGAAGATCGAAAGGTATTTGGACGCAATCCGAGAGCGCGTGAACTGCCGCGTGAACATATTCCACGGCGGCGACGACGACGTGATTCCGTTGGAGTGCAGCTACAACGTGAAGGCGCGTGTGCCGAGGGCGCGTGTGAATGTGGTGCAAAACAAAGACCACATCACAATCGTCGTGGGAAGACAAAAAGCCTTTGCCAGAGAGCTTGAGCAAATTTGGAGTAACTCAAATGCTTCAATTTAAAATAAATTCAAGCAAATATTGTTATTCTCATTATCAATATATCTTAATCGTAAATTAAAAGTTTTATATAAAAAAAAAATCTTATTCTCAAGAAAAAGATTAAAGATTTAAATAACCTGAAAAGCAGGTTTTTTTTTTTTTTTTTTTTGTGATCTTCTTCATGTGTGAAAAGTCTGTATTTTGTGGGGGCAAAGCCGTG

Coding sequence (CDS)

ATGGCCACCAAATCATCAGCCAAATGGGTCTTCATCACGCTCGCCCGTTTCTCCAACGAAGCCCTCAGCTTCTTCCTCTTCTCTCTTCTCGACCTCCTCGACATTCTCCTCTGCTTTCTCTACAAACTCGCTGATTTCTTCTTTGAATCCGAGTGGAAGCCTTGCTATTGCTCCTCTCATCAGGAGTCTTTTGCCAGTAGTAAGATTTTGGTCTCCCAGAAGGCTCTCTCTTCCTCCACTAAGCTTCATCTCGAGCAACTTTCTGATACTCTCTACACTCGTCCTTCTCTCCTCTCCGAGCTCTCCAAATTCACTGTCAATGAGCTCCGCCGCCTCAAGGTTAAGCCTTTTCTTCTTCGCTCCTCCGCCGCGGCCCCGGTCGGTTCCACGTTTTCGGTTAACTCTACCATTGTTGAGATGCTTCAGGAGAAGATCAATGGCGGACAGAGTCCTAGGTGGTCTGAGTGTAATTGTAAACTTTGTACTTGTTGGTCTTCTCCCTCTAAACAATCGCTCTTCGTTCGTTCTCAAGGACCTAGAGATCAGCCACGTGAAGACGTACTGTTCATTCATGGCTTCATTTCTTCATCCGCGTTCTGGACGGAAACCCTCTTCCCAAACTTCTCGGCGTCCGCCAAATCGACATATCGGCTCCTCGCTGTCGATCTGCTCGGATTCGGTCGGAGCCCGAAGCCAGCTGATTCACTCTACACATTGAAGGAGCACGTCGACATGATCGAAACCTCAGTTCTTGAAGCCTACAAGGTCAAGTCCTTCCACATCGTCGCTCATTCCCTTGGCTGCATTTTGGCCCTGGCTTTAGCCGTCAAGCATCCCGGCTCAGTCAAGTCACTAACATTACTTGCACCGCCTTATTATCCCGTGCCAAAAGGGGAAGAGGCGAGCCAATACGTGATGAGGAAGGTGGCGCCGCGGCGGGTGTGGCCGCCGATAGCGCTGGGGGCGTCGATTGCTTGCTGGTACGAGCACATCTCAAGGACGGTGTGCCTACTCATATGCAAGAACCATCGCTTTTGGGAGTTTCTAACCAAACTTGTCACAAGAAACAGGATAGAGTCATTTTTGGTGGAGGGATTCTTCGCCCACACGCACAACGCCGCGTGGCATACACTGCACAACGTGATATGCGGTACCGGCGGGAAGATCGAAAGGTATTTGGACGCAATCCGAGAGCGCGTGAACTGCCGCGTGAACATATTCCACGGCGGCGACGACGACGTGATTCCGTTGGAGTGCAGCTACAACGTGAAGGCGCGTGTGCCGAGGGCGCGTGTGAATGTGGTGCAAAACAAAGACCACATCACAATCGTCGTGGGAAGACAAAAAGCCTTTGCCAGAGAGCTTGAGCAAATTTGGAGTAACTCAAATGCTTCAATTTAA

Protein sequence

MATKSSAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASSKILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSSAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRDQPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNASI
Homology
BLAST of Lcy02g012520 vs. ExPASy Swiss-Prot
Match: O22977 (Probable lysophospholipase BODYGUARD 3 OS=Arabidopsis thaliana OX=3702 GN=BDG3 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.0e-160
Identity = 287/481 (59.67%), Postives = 360/481 (74.84%), Query Frame = 0

Query: 19  NEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASS--KILVSQ--- 78
           NEA+SF +F +LD++D  LC LYK AD+ FE+EWKPCYC S +E   ++  KIL+S    
Sbjct: 18  NEAVSFVVFCILDIVDSFLCLLYKAADYLFEAEWKPCYCLSDKEPITTTRGKILLSHNNG 77

Query: 79  ----------KALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSSA 138
                     + L   +K+ LE +S+TLYTRPSL+S++S  +VNEL +  VK     S  
Sbjct: 78  ESKILTLSPLQELGGRSKIELEDISETLYTRPSLISDISTISVNELNKRFVKVTRSESEC 137

Query: 139 A------------APVGSTFSVNSTIVEMLQEKIN----GGQSPRWSECNCKLCTCWSSP 198
           +            +   S+ +VN T+VEML+ KI          RWS+C+C  CT W+S 
Sbjct: 138 SGHNEKTKNKRRRSLTKSSLTVNFTVVEMLRGKIRPQNLNHDISRWSDCDCGFCTSWAST 197

Query: 199 S--KQSLFVRSQGPRD-QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLL 258
           S    SLFV++Q P     +EDVLFIHGFISSSAFWTET+FP+ SAS+ ST+RL AVDLL
Sbjct: 198 SDKNHSLFVKTQIPNGVTAKEDVLFIHGFISSSAFWTETVFPSLSASS-STHRLFAVDLL 257

Query: 259 GFGRSPKPADSLYTLKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKS 318
           GFG+SPKPADSLYTL+EHV+MIE SVL  Y VKSFHIVAHSLGCILAL+LA +H G +KS
Sbjct: 258 GFGKSPKPADSLYTLREHVEMIEKSVLHKYNVKSFHIVAHSLGCILALSLAARHGGLIKS 317

Query: 319 LTLLAPPYYPVPKGE-EASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNH 378
           LTLLAPPYYPVPKGE +  QYVM+KVAPR+VWPPIALGAS+ACWYEHISRT+CLLICK+H
Sbjct: 318 LTLLAPPYYPVPKGEKKPRQYVMKKVAPRKVWPPIALGASMACWYEHISRTICLLICKHH 377

Query: 379 RFWEFLTKLVTR-NRIESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNC 438
           R W+F+  ++TR NR  +FL+EGF  HTHNAAWHTLHN+ICGTG K++ YLD +R+++ C
Sbjct: 378 RVWQFIAGVLTRNNRTVNFLIEGFMCHTHNAAWHTLHNIICGTGSKLDTYLDIVRDKLKC 437

Query: 439 RVNIFHGGDDDVIPLECSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNS 464
            V IFHGGDD++IP+ECSYNVK R+PRARV V+++KDHIT+VVGRQ  FAREL++IW  S
Sbjct: 438 NVTIFHGGDDELIPVECSYNVKQRIPRARVKVIEHKDHITMVVGRQDEFARELQEIWKTS 497

BLAST of Lcy02g012520 vs. ExPASy Swiss-Prot
Match: Q8LFX7 (Probable lysophospholipase BODYGUARD 1 OS=Arabidopsis thaliana OX=3702 GN=BDG1 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.3e-156
Identity = 275/464 (59.27%), Postives = 352/464 (75.86%), Query Frame = 0

Query: 15  ARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASS------- 74
           +R  N  +  F+F +LD++D LLCF YK  DFFFESEWKPCYC    E+   S       
Sbjct: 4   SRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRGG 63

Query: 75  KILVSQKALSSS-------TKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLR 134
           K++VS+++   S       TK++L+++SDTLY+RPSLL++L+K  V   ++  VK     
Sbjct: 64  KMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKL-VKCFKKDVVKCCDES 123

Query: 135 SSAAAPVGST-FSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGP 194
              +     T  +VNST+VE LQ      ++PRWS+C+C  CT W S S QSLFV  Q P
Sbjct: 124 KKRSPSTKKTLLTVNSTVVEKLQ------RTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP 183

Query: 195 RD-QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYT 254
            D + +E+V+FIHGF+SSS FWTETLFPNFS SAKS YR LAVDLLG+G+SPKP DSLYT
Sbjct: 184 TDNKAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYT 243

Query: 255 LKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG 314
           LKEH++MIE SV+  +++K+FH+VAHSLGCILALALAVKHPG++KSLTLLAPPYY VPKG
Sbjct: 244 LKEHLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKG 303

Query: 315 EEASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRI 374
            + +QYVMR++AP+ VWPP+A GAS+A WYEHISRTV L++CKNH   EFLT+L+TRNR+
Sbjct: 304 VQGTQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRM 363

Query: 375 ESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLE 434
            ++L+EGF  HTHNA+WHTLHN+I G+G K+E YLD +R+ V+C V +FHGG D++IP+E
Sbjct: 364 RTYLIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVE 423

Query: 435 CSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNS 463
           CSY VK +VPRAR++VV +KDHITIVVGRQK FARELE IW  S
Sbjct: 424 CSYGVKRKVPRARIHVVPDKDHITIVVGRQKEFARELELIWRRS 460

BLAST of Lcy02g012520 vs. ExPASy Swiss-Prot
Match: Q9FJ24 (Probable lysophospholipase BODYGUARD 2 OS=Arabidopsis thaliana OX=3702 GN=BDG2 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.9e-147
Identity = 260/476 (54.62%), Postives = 352/476 (73.95%), Query Frame = 0

Query: 14  LARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA-SSKILVS 73
           +AR+ N  + FF+F+LLD+ D LLC+ YK  D+F ESE KPCYCSS  E+ A + KI+VS
Sbjct: 3   IARWLNRTVGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVS 62

Query: 74  QKALSSS------TKLHLEQLSDTLYTR-PSLLSELSKFTVNELRRLKVKPFLLRSSAAA 133
           ++   S       +K+H +++SDTLY+R PSLL+ LSK  V  ++    K  ++R +   
Sbjct: 63  ERGGYSKVVSLTRSKIHFDEISDTLYSRGPSLLTRLSKL-VRSVKCFNYKGLIMRGNVVE 122

Query: 134 PVG-------------STFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCW-SSPSKQS 193
                              ++NST++E          +PRWS+C+C  CT W +S ++ S
Sbjct: 123 SCDHHESKKKISKGKKRLMTLNSTVIE------KSSTAPRWSDCHCSFCTSWLTSTNRDS 182

Query: 194 LFVRSQGPRD--QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRS 253
           LFV+ Q P+D  + R++V+FIHGF+SSSAFWTETLFPNFS SAKS YR +AVDLLG+GRS
Sbjct: 183 LFVKVQQPKDNKKARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRS 242

Query: 254 PKPADSLYTLKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLA 313
           PKP DSLYTL+EH++MIE SV+  +K+K+FHIVAHSLGCILALALAVKHPG++KSLTLLA
Sbjct: 243 PKPNDSLYTLREHLEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLA 302

Query: 314 PPYYPVPKGEEASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFL 373
           PPYY VPKG + +QYVMR+VA + VWPP+  GAS+  WYEH+ RT+ L++ KNH+  EF+
Sbjct: 303 PPYYKVPKGVQPAQYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFV 362

Query: 374 TKLVTRNRIESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHG 433
           T+L+T NR+ ++L+EGF  HTHN ++HTLHN+I G+G K++ YLD +R+ V+C V IFHG
Sbjct: 363 TRLLTLNRMRTYLIEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHG 422

Query: 434 GDDDVIPLECSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNAS 466
           G D++IP+ECSY+VK++VPRA V+V+ +KDHITIVVGRQK FARELE IW  + ++
Sbjct: 423 GKDELIPVECSYSVKSKVPRATVHVIPDKDHITIVVGRQKDFARELELIWQRTKST 471

BLAST of Lcy02g012520 vs. ExPASy Swiss-Prot
Match: Q700D5 (Probable lysophospholipase BODYGUARD 4 OS=Arabidopsis thaliana OX=3702 GN=BDG4 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.9e-87
Identity = 177/446 (39.69%), Postives = 256/446 (57.40%), Query Frame = 0

Query: 21  ALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASSKILVSQKALSSST 80
           ALSF +F  LDLLD +LC +Y+  D   E     CYC++               A  S T
Sbjct: 14  ALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTA--------------AAPQSQT 73

Query: 81  KLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVK-PFLLRSSAAAPVGSTFSVNSTIVE 140
               E  S+TL+ R ++   +  + +   R  K K    LR S                +
Sbjct: 74  TDENELSSETLFGRRNIFRGM--WFLGFAREFKSKLSRKLRKS----------------K 133

Query: 141 MLQEKINGGQSPRWSECNCKLCTCWSSPSKQSL--FVRSQGPRD----QPREDVLFIHGF 200
           + QE +N     RWS+C CK C  W+     +L   V+    R+    +P E+V+FIHGF
Sbjct: 134 IHQESVN-----RWSDCGCKSCKSWTKNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGF 193

Query: 201 ISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEHVDMIETSVLEA 260
           + SS FWTET+F +     K  YRLLA+DLLGFG SPKP DSLYTLK+HVD IE SV++ 
Sbjct: 194 MGSSHFWTETVFEHIQ---KDDYRLLAIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKP 253

Query: 261 YKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEASQYVMRKVAPRR 320
           Y++ SFH+VAHS+GC++ALALA KH   VKS+TL+APPY+P          V+ ++A +R
Sbjct: 254 YQLDSFHVVAHSMGCLIALALAAKHSNIVKSVTLVAPPYFP----SSVEGSVLNRIARKR 313

Query: 321 VWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFLVEGFFAHTHNA 380
           +WPP+A G ++  WYEHI R VC +ICK+H+ WE+L KL    R   + ++    HTH++
Sbjct: 314 LWPPLAFGTAVMSWYEHIGRCVCFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHS 373

Query: 381 AWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYNVKARVPRARVN 440
           AWH++HNVICG     + +L+ +  +   ++++  G  D ++P  CS N+K   P   V+
Sbjct: 374 AWHSMHNVICGGSKVADEHLETL-IKSGVKIHLMQGDCDQIVPSHCSGNMKRTFPAVEVD 414

Query: 441 VVQNKDHITIVVGRQKAFARELEQIW 460
           ++   DH +++ GR + FA +LE IW
Sbjct: 434 IITGADHDSMISGRGEEFAEKLESIW 414

BLAST of Lcy02g012520 vs. ExPASy Swiss-Prot
Match: Q9FN79 (Probable lysophospholipase BODYGUARD 5 OS=Arabidopsis thaliana OX=3702 GN=BDG5 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.8e-82
Identity = 163/451 (36.14%), Postives = 253/451 (56.10%), Query Frame = 0

Query: 19  NEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASSKILVSQKALSS 78
           N A S+ +F L DLLD  LC +++  D   E + + C+C++ +E    ++          
Sbjct: 15  NGAPSWAVFFLFDLLDYFLCIVFRFLDEVMEEKSESCHCNNPREKTHFAE---------- 74

Query: 79  STKLHLEQLSDTLYTRPSLLSELSKFT-VNELRRLKVKPFLLRSSAAAPVGSTFSVNSTI 138
                 E LS+TLY R ++  +       N+L  +  K           +G    +   +
Sbjct: 75  -----YEFLSETLYRRRNVFRQAGFLRFANKLPEITKK-----------IGIVTFLRKFL 134

Query: 139 VEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD-----QPREDVLFIHG 198
                 K++   + RWS+C CK C  W +  K ++ V+     D     +P E+V+F+HG
Sbjct: 135 FPHTMNKVSHEVANRWSDCGCKTCVSWINTDKLNVIVKQPSISDLSISNKPVENVIFVHG 194

Query: 199 FISSSAFWTETLFPNFSASAKST-YRLLAVDLLGFGRSPKPADSLYTLKEHVDMIETSVL 258
           F++SS+FWT T+F     + + T YR  A+DLLGFG SPKP  S Y+LKEHV+MIE SV+
Sbjct: 195 FLASSSFWTNTVFKYLPETTEGTNYRFFAIDLLGFGDSPKPRASQYSLKEHVEMIEKSVI 254

Query: 259 EAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEASQYVMRKVAP 318
               + SFH+VAHS+GCI+ +ALA K   SVKS+ L+APPY+   KG  AS   +  VA 
Sbjct: 255 LPNNLTSFHVVAHSMGCIIGIALAAKFSDSVKSVALVAPPYFADSKG-GASCAALDVVAK 314

Query: 319 RRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFLVEGFFAHTH 378
           +++WPP +   ++ CWYEHI R VCL+ C++HR WE + K+VT  R     +  F  HTH
Sbjct: 315 KKLWPPASFFTAMMCWYEHIGRGVCLVFCRHHRTWERIIKIVTWRRKLPTAIMDFTKHTH 374

Query: 379 NAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYNVKARVPRAR 438
            + WH++HNVICG     +++L+ +  +   ++N+  G  D V+P++C  N+K + P   
Sbjct: 375 QSGWHSMHNVICGGAKFTDKHLETL-IKSGVKINVMQGDKDVVVPIDCLSNMKGKFPAVE 434

Query: 439 VNVVQNKDHITIVVGRQKAFARELEQIWSNS 463
           V V+   DH T+++ R++ FA  L  +W+ S
Sbjct: 435 VEVIAGTDHSTVIMSRREVFAANLVSLWATS 437

BLAST of Lcy02g012520 vs. ExPASy TrEMBL
Match: A0A6J1GZT8 (probable lysophospholipase BODYGUARD 3 OS=Cucurbita moschata OX=3662 GN=LOC111459152 PE=4 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.7e-236
Identity = 407/464 (87.72%), Postives = 437/464 (94.18%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPF++ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFVIGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A VGSTF+VNSTIVEMLQEKINGGQSPRWSECNCKLC+CWSS +KQSLFVRSQGP+D
Sbjct: 131 TANAAVGSTFTVNSTIVEMLQEKINGGQSPRWSECNCKLCSCWSSSTKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            PREDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPREDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+TRNRIES
Sbjct: 311 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLITRNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IW NSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWRNSNA 474

BLAST of Lcy02g012520 vs. ExPASy TrEMBL
Match: A0A6J1K6F8 (probable lysophospholipase BODYGUARD 3 OS=Cucurbita maxima OX=3661 GN=LOC111491586 PE=4 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.1e-234
Identity = 405/464 (87.28%), Postives = 434/464 (93.53%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPF++ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFVIGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A V STF+VNSTIVEMLQEKINGGQS RWSECNCKLC+CWSS SKQSLFVRSQGP+D
Sbjct: 131 TAKAAVRSTFTVNSTIVEMLQEKINGGQSRRWSECNCKLCSCWSSSSKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            P EDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPHEDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+ RNRIES
Sbjct: 311 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLIARNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IWSNSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWSNSNA 474

BLAST of Lcy02g012520 vs. ExPASy TrEMBL
Match: A0A6J1I4D0 (probable lysophospholipase BODYGUARD 1 OS=Cucurbita maxima OX=3661 GN=LOC111469048 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.4e-224
Identity = 382/462 (82.68%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           +AKWV I++A F+NEALS F+F+LLDL+D  LCF+YK+ADFFFESEWK CYCSSH+E+ +
Sbjct: 12  TAKWVLISIAHFTNEALSIFVFNLLDLIDFGLCFVYKVADFFFESEWKACYCSSHKEAIS 71

Query: 66  S---SKILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSS 125
           S   +KILVS+KALS STKL LE++SDTLYTRPSLL+ELSK  V ELRRLKVKPF++ S+
Sbjct: 72  SNDGNKILVSEKALSLSTKLQLEEVSDTLYTRPSLLTELSKVAVAELRRLKVKPFVVGST 131

Query: 126 AAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRDQ 185
             A VGSTF+VNSTIVEML+EKIN GQ+PRWSEC+C+ CT   S SKQSLFVRSQGP+D 
Sbjct: 132 GKAAVGSTFAVNSTIVEMLKEKINVGQNPRWSECDCQRCTSCCSSSKQSLFVRSQGPQDN 191

Query: 186 PREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEH 245
           PREDVLFIHGF+SSS FWTETLFPNFSASAKSTYR LAVDLLGFG SP+P DSLYTLKEH
Sbjct: 192 PREDVLFIHGFLSSSTFWTETLFPNFSASAKSTYRFLAVDLLGFGLSPRPTDSLYTLKEH 251

Query: 246 VDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEAS 305
           VDMIETS+LEAYKVKSFHIVAHSLGCILALALAVKHP SVKSLTLLAPPYYP+PKGEE S
Sbjct: 252 VDMIETSILEAYKVKSFHIVAHSLGCILALALAVKHPSSVKSLTLLAPPYYPIPKGEEPS 311

Query: 306 QYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFL 365
           QYVMRKVAPRRVWPPIALG+SIACWYEHISRTVCL+ICKNHRFWEFLTK VTRNRIESFL
Sbjct: 312 QYVMRKVAPRRVWPPIALGSSIACWYEHISRTVCLIICKNHRFWEFLTKFVTRNRIESFL 371

Query: 366 VEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYN 425
           VEGFFAHTHNAAWHTLHNVICGTGGKIERYLD +RE+V+C+VNI HGGDDDV+P+ECSYN
Sbjct: 372 VEGFFAHTHNAAWHTLHNVICGTGGKIERYLDELREQVSCQVNIIHGGDDDVVPVECSYN 431

Query: 426 VKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           VKARVPRARVNVVQNKDHITIV+GR++AFARELE+IWSN+ +
Sbjct: 432 VKARVPRARVNVVQNKDHITIVIGRRQAFARELEEIWSNAKS 473

BLAST of Lcy02g012520 vs. ExPASy TrEMBL
Match: A0A6J1EM17 (probable lysophospholipase BODYGUARD 3 OS=Cucurbita moschata OX=3662 GN=LOC111435639 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.5e-222
Identity = 378/462 (81.82%), Postives = 421/462 (91.13%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           +AKWV I++A F+NEALS  +F+LLDL+D  LCF+YK+ADFFFESEWK CYC SH+E+ +
Sbjct: 12  TAKWVLISIAHFTNEALSIIVFNLLDLIDFGLCFVYKVADFFFESEWKACYCCSHKEAIS 71

Query: 66  SS---KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSS 125
           SS   KILVS+KALS STKL LE++SDTLYTRPSLL+ELSK  V ELRRLKVKPF++ S+
Sbjct: 72  SSDGNKILVSEKALSLSTKLQLEEVSDTLYTRPSLLTELSKVAVAELRRLKVKPFVVGST 131

Query: 126 AAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRDQ 185
             A VGSTF+VNST+VEML+EKIN GQ+PRWSEC+CK CT   S SKQSLFVRSQGP+D 
Sbjct: 132 GKAAVGSTFAVNSTVVEMLKEKINVGQNPRWSECDCKRCTSCCSSSKQSLFVRSQGPQDN 191

Query: 186 PREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEH 245
           PREDVLFIHGF+SSS FWTETLFPNFSASA STYR LAVDLLGFG SP+P DSLYTLKEH
Sbjct: 192 PREDVLFIHGFLSSSTFWTETLFPNFSASANSTYRFLAVDLLGFGLSPRPTDSLYTLKEH 251

Query: 246 VDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEAS 305
           VDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHP SVKSLTLLAPPYYP+PKGEE S
Sbjct: 252 VDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPSSVKSLTLLAPPYYPIPKGEEPS 311

Query: 306 QYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFL 365
           QYVMRKVAPRRVWPPI LG+SIACWYEHISRTVCL+ICKNHRFWEFLT  VTRNRIESFL
Sbjct: 312 QYVMRKVAPRRVWPPIVLGSSIACWYEHISRTVCLIICKNHRFWEFLTIFVTRNRIESFL 371

Query: 366 VEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYN 425
           VEGFFAHTHNAAWHTLHN+ICGTGGKI+RYLD +RE+VNC+VNI HGGDDDV+P+ECSYN
Sbjct: 372 VEGFFAHTHNAAWHTLHNIICGTGGKIDRYLDQVREQVNCQVNIIHGGDDDVVPVECSYN 431

Query: 426 VKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           VKARVPRARVNVVQNKDHITIV+GR++AFARELE+IWSN+ +
Sbjct: 432 VKARVPRARVNVVQNKDHITIVIGRRQAFARELEEIWSNAKS 473

BLAST of Lcy02g012520 vs. ExPASy TrEMBL
Match: A0A5A7TLN8 (Alpha/beta hydrolase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001490 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.4e-217
Identity = 379/465 (81.51%), Postives = 416/465 (89.46%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV  ++  F+NE LS FLFSLLD++DI+LCFLYK+ADFFFES+WKPCYCSSH+E+  
Sbjct: 4   SAKWVLKSIGNFTNEILSIFLFSLLDIIDIVLCFLYKMADFFFESQWKPCYCSSHKEAIT 63

Query: 66  S---SKILVSQ-KALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           S   +K+LVSQ   LS STKL LE++SDTLYTRPS LSE+S          KV PF++ S
Sbjct: 64  SCDGNKVLVSQNNVLSLSTKLQLEEVSDTLYTRPSYLSEIS----------KVMPFVIGS 123

Query: 126 --SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGP 185
             +A   VGSTF+V+STIVEMLQ+KINGGQ+PRWS+C+CK CT WSS  KQSL+VRS+G 
Sbjct: 124 PTTAKTTVGSTFTVHSTIVEMLQDKINGGQNPRWSDCDCKSCTRWSSSPKQSLYVRSEGV 183

Query: 186 RDQPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTL 245
           RD PREDVLFIHGFISSSAFWTETLFPNFSASAKS+YR LAVDLLGFG+SPKPADSLYTL
Sbjct: 184 RDHPREDVLFIHGFISSSAFWTETLFPNFSASAKSSYRFLAVDLLGFGQSPKPADSLYTL 243

Query: 246 KEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGE 305
           KEHVDMIE SVL+AYKVKSFHIVAHSLGCILALALAVKHPGS+KSLTLLAPPYYPVPKG 
Sbjct: 244 KEHVDMIEASVLDAYKVKSFHIVAHSLGCILALALAVKHPGSIKSLTLLAPPYYPVPKGV 303

Query: 306 EASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIE 365
           E SQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLT+LVTRNRIE
Sbjct: 304 EPSQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTQLVTRNRIE 363

Query: 366 SFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLEC 425
           SFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDA+RERVNC VNIFHG DDDV+P+EC
Sbjct: 364 SFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAVRERVNCHVNIFHGSDDDVVPVEC 423

Query: 426 SYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           S+ VKARVP ARVNVVQNKDHITIV+GRQKAFARELE+IWS SNA
Sbjct: 424 SHGVKARVPTARVNVVQNKDHITIVIGRQKAFARELEEIWSKSNA 458

BLAST of Lcy02g012520 vs. NCBI nr
Match: XP_022957687.1 (probable lysophospholipase BODYGUARD 3 [Cucurbita moschata])

HSP 1 Score: 828.2 bits (2138), Expect = 3.6e-236
Identity = 407/464 (87.72%), Postives = 437/464 (94.18%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPF++ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFVIGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A VGSTF+VNSTIVEMLQEKINGGQSPRWSECNCKLC+CWSS +KQSLFVRSQGP+D
Sbjct: 131 TANAAVGSTFTVNSTIVEMLQEKINGGQSPRWSECNCKLCSCWSSSTKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            PREDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPREDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+TRNRIES
Sbjct: 311 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLITRNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IW NSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWRNSNA 474

BLAST of Lcy02g012520 vs. NCBI nr
Match: XP_023534416.1 (probable lysophospholipase BODYGUARD 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.3 bits (2128), Expect = 5.2e-235
Identity = 406/464 (87.50%), Postives = 435/464 (93.75%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPFL+ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFLIGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A V STF+VNSTIV+MLQEKINGGQSPRWSECNCKLC+CWSS SKQSLFVRSQGP+D
Sbjct: 131 TANAAVRSTFTVNSTIVDMLQEKINGGQSPRWSECNCKLCSCWSSSSKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            P EDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPHEDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+TRNRIES
Sbjct: 311 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLITRNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IW NSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWRNSNA 474

BLAST of Lcy02g012520 vs. NCBI nr
Match: KAG7035517.1 (putative lysophospholipase BODYGUARD 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 821.6 bits (2121), Expect = 3.4e-234
Identity = 404/464 (87.07%), Postives = 435/464 (93.75%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPF++ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFVVGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A V STF+VNSTIVEMLQEKINGGQSPRWSECNCKLC+CWSS +KQSLFVRSQGP+D
Sbjct: 131 TANAAVRSTFTVNSTIVEMLQEKINGGQSPRWSECNCKLCSCWSSSTKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            P EDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPHEDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           +SQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+TRNRIES
Sbjct: 311 SSQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLITRNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IW NSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWRNSNA 474

BLAST of Lcy02g012520 vs. NCBI nr
Match: XP_022996320.1 (probable lysophospholipase BODYGUARD 3 [Cucurbita maxima])

HSP 1 Score: 821.2 bits (2120), Expect = 4.4e-234
Identity = 405/464 (87.28%), Postives = 434/464 (93.53%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           SAKWV IT+ARFSNEALSFFLFSLLD++D+LLCF+YK+ADFF+ESEWKPCYCSSH +   
Sbjct: 11  SAKWVLITIARFSNEALSFFLFSLLDVIDVLLCFVYKVADFFYESEWKPCYCSSHDQEAI 70

Query: 66  SS----KILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRS 125
           SS    KILVS+K LS STKL LE++SDTLYTRPSLLS+LSK TVNELRRLKVKPF++ S
Sbjct: 71  SSGDGNKILVSEKGLSLSTKLKLEEVSDTLYTRPSLLSDLSKVTVNELRRLKVKPFVIGS 130

Query: 126 SAAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRD 185
           +A A V STF+VNSTIVEMLQEKINGGQS RWSECNCKLC+CWSS SKQSLFVRSQGP+D
Sbjct: 131 TAKAAVRSTFTVNSTIVEMLQEKINGGQSRRWSECNCKLCSCWSSSSKQSLFVRSQGPKD 190

Query: 186 QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKE 245
            P EDVLFIHGFISSSAFWTETLFP+FSASAKS YRLLAVDLLGFGRSPKPA+SLYTLKE
Sbjct: 191 HPHEDVLFIHGFISSSAFWTETLFPHFSASAKSAYRLLAVDLLGFGRSPKPANSLYTLKE 250

Query: 246 HVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG-EE 305
           HVDMIE+SVLE +KVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG EE
Sbjct: 251 HVDMIESSVLETHKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEE 310

Query: 306 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIES 365
           ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKL+ RNRIES
Sbjct: 311 ASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLIARNRIES 370

Query: 366 FLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECS 425
           FLVEGFFAHTHNAAWHTLHNVICGTGGK+ERYLD IRE V C VNIFHGGDDDV+P+ECS
Sbjct: 371 FLVEGFFAHTHNAAWHTLHNVICGTGGKMERYLDIIRETVKCEVNIFHGGDDDVVPVECS 430

Query: 426 YNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           YN+KARVPRARVNVVQNKDHITIV+GR+KAFARELE IWSNSNA
Sbjct: 431 YNLKARVPRARVNVVQNKDHITIVMGRRKAFARELEGIWSNSNA 474

BLAST of Lcy02g012520 vs. NCBI nr
Match: XP_022970039.1 (probable lysophospholipase BODYGUARD 1 [Cucurbita maxima])

HSP 1 Score: 787.3 bits (2032), Expect = 7.1e-224
Identity = 382/462 (82.68%), Postives = 426/462 (92.21%), Query Frame = 0

Query: 6   SAKWVFITLARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA 65
           +AKWV I++A F+NEALS F+F+LLDL+D  LCF+YK+ADFFFESEWK CYCSSH+E+ +
Sbjct: 12  TAKWVLISIAHFTNEALSIFVFNLLDLIDFGLCFVYKVADFFFESEWKACYCSSHKEAIS 71

Query: 66  S---SKILVSQKALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSS 125
           S   +KILVS+KALS STKL LE++SDTLYTRPSLL+ELSK  V ELRRLKVKPF++ S+
Sbjct: 72  SNDGNKILVSEKALSLSTKLQLEEVSDTLYTRPSLLTELSKVAVAELRRLKVKPFVVGST 131

Query: 126 AAAPVGSTFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGPRDQ 185
             A VGSTF+VNSTIVEML+EKIN GQ+PRWSEC+C+ CT   S SKQSLFVRSQGP+D 
Sbjct: 132 GKAAVGSTFAVNSTIVEMLKEKINVGQNPRWSECDCQRCTSCCSSSKQSLFVRSQGPQDN 191

Query: 186 PREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEH 245
           PREDVLFIHGF+SSS FWTETLFPNFSASAKSTYR LAVDLLGFG SP+P DSLYTLKEH
Sbjct: 192 PREDVLFIHGFLSSSTFWTETLFPNFSASAKSTYRFLAVDLLGFGLSPRPTDSLYTLKEH 251

Query: 246 VDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEAS 305
           VDMIETS+LEAYKVKSFHIVAHSLGCILALALAVKHP SVKSLTLLAPPYYP+PKGEE S
Sbjct: 252 VDMIETSILEAYKVKSFHIVAHSLGCILALALAVKHPSSVKSLTLLAPPYYPIPKGEEPS 311

Query: 306 QYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFL 365
           QYVMRKVAPRRVWPPIALG+SIACWYEHISRTVCL+ICKNHRFWEFLTK VTRNRIESFL
Sbjct: 312 QYVMRKVAPRRVWPPIALGSSIACWYEHISRTVCLIICKNHRFWEFLTKFVTRNRIESFL 371

Query: 366 VEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYN 425
           VEGFFAHTHNAAWHTLHNVICGTGGKIERYLD +RE+V+C+VNI HGGDDDV+P+ECSYN
Sbjct: 372 VEGFFAHTHNAAWHTLHNVICGTGGKIERYLDELREQVSCQVNIIHGGDDDVVPVECSYN 431

Query: 426 VKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNA 465
           VKARVPRARVNVVQNKDHITIV+GR++AFARELE+IWSN+ +
Sbjct: 432 VKARVPRARVNVVQNKDHITIVIGRRQAFARELEEIWSNAKS 473

BLAST of Lcy02g012520 vs. TAIR 10
Match: AT4G24140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.4e-161
Identity = 287/481 (59.67%), Postives = 360/481 (74.84%), Query Frame = 0

Query: 19  NEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASS--KILVSQ--- 78
           NEA+SF +F +LD++D  LC LYK AD+ FE+EWKPCYC S +E   ++  KIL+S    
Sbjct: 18  NEAVSFVVFCILDIVDSFLCLLYKAADYLFEAEWKPCYCLSDKEPITTTRGKILLSHNNG 77

Query: 79  ----------KALSSSTKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLRSSA 138
                     + L   +K+ LE +S+TLYTRPSL+S++S  +VNEL +  VK     S  
Sbjct: 78  ESKILTLSPLQELGGRSKIELEDISETLYTRPSLISDISTISVNELNKRFVKVTRSESEC 137

Query: 139 A------------APVGSTFSVNSTIVEMLQEKIN----GGQSPRWSECNCKLCTCWSSP 198
           +            +   S+ +VN T+VEML+ KI          RWS+C+C  CT W+S 
Sbjct: 138 SGHNEKTKNKRRRSLTKSSLTVNFTVVEMLRGKIRPQNLNHDISRWSDCDCGFCTSWAST 197

Query: 199 S--KQSLFVRSQGPRD-QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLL 258
           S    SLFV++Q P     +EDVLFIHGFISSSAFWTET+FP+ SAS+ ST+RL AVDLL
Sbjct: 198 SDKNHSLFVKTQIPNGVTAKEDVLFIHGFISSSAFWTETVFPSLSASS-STHRLFAVDLL 257

Query: 259 GFGRSPKPADSLYTLKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKS 318
           GFG+SPKPADSLYTL+EHV+MIE SVL  Y VKSFHIVAHSLGCILAL+LA +H G +KS
Sbjct: 258 GFGKSPKPADSLYTLREHVEMIEKSVLHKYNVKSFHIVAHSLGCILALSLAARHGGLIKS 317

Query: 319 LTLLAPPYYPVPKGE-EASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNH 378
           LTLLAPPYYPVPKGE +  QYVM+KVAPR+VWPPIALGAS+ACWYEHISRT+CLLICK+H
Sbjct: 318 LTLLAPPYYPVPKGEKKPRQYVMKKVAPRKVWPPIALGASMACWYEHISRTICLLICKHH 377

Query: 379 RFWEFLTKLVTR-NRIESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNC 438
           R W+F+  ++TR NR  +FL+EGF  HTHNAAWHTLHN+ICGTG K++ YLD +R+++ C
Sbjct: 378 RVWQFIAGVLTRNNRTVNFLIEGFMCHTHNAAWHTLHNIICGTGSKLDTYLDIVRDKLKC 437

Query: 439 RVNIFHGGDDDVIPLECSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNS 464
            V IFHGGDD++IP+ECSYNVK R+PRARV V+++KDHIT+VVGRQ  FAREL++IW  S
Sbjct: 438 NVTIFHGGDDELIPVECSYNVKQRIPRARVKVIEHKDHITMVVGRQDEFARELQEIWKTS 497

BLAST of Lcy02g012520 vs. TAIR 10
Match: AT1G64670.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 554.3 bits (1427), Expect = 9.4e-158
Identity = 275/464 (59.27%), Postives = 352/464 (75.86%), Query Frame = 0

Query: 15  ARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASS------- 74
           +R  N  +  F+F +LD++D LLCF YK  DFFFESEWKPCYC    E+   S       
Sbjct: 4   SRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRGG 63

Query: 75  KILVSQKALSSS-------TKLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVKPFLLR 134
           K++VS+++   S       TK++L+++SDTLY+RPSLL++L+K  V   ++  VK     
Sbjct: 64  KMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKL-VKCFKKDVVKCCDES 123

Query: 135 SSAAAPVGST-FSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFVRSQGP 194
              +     T  +VNST+VE LQ      ++PRWS+C+C  CT W S S QSLFV  Q P
Sbjct: 124 KKRSPSTKKTLLTVNSTVVEKLQ------RTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP 183

Query: 195 RD-QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYT 254
            D + +E+V+FIHGF+SSS FWTETLFPNFS SAKS YR LAVDLLG+G+SPKP DSLYT
Sbjct: 184 TDNKAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYT 243

Query: 255 LKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKG 314
           LKEH++MIE SV+  +++K+FH+VAHSLGCILALALAVKHPG++KSLTLLAPPYY VPKG
Sbjct: 244 LKEHLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKG 303

Query: 315 EEASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRI 374
            + +QYVMR++AP+ VWPP+A GAS+A WYEHISRTV L++CKNH   EFLT+L+TRNR+
Sbjct: 304 VQGTQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRM 363

Query: 375 ESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLE 434
            ++L+EGF  HTHNA+WHTLHN+I G+G K+E YLD +R+ V+C V +FHGG D++IP+E
Sbjct: 364 RTYLIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVE 423

Query: 435 CSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNS 463
           CSY VK +VPRAR++VV +KDHITIVVGRQK FARELE IW  S
Sbjct: 424 CSYGVKRKVPRARIHVVPDKDHITIVVGRQKEFARELELIWRRS 460

BLAST of Lcy02g012520 vs. TAIR 10
Match: AT5G41900.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.4e-148
Identity = 260/476 (54.62%), Postives = 352/476 (73.95%), Query Frame = 0

Query: 14  LARFSNEALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFA-SSKILVS 73
           +AR+ N  + FF+F+LLD+ D LLC+ YK  D+F ESE KPCYCSS  E+ A + KI+VS
Sbjct: 3   IARWLNRTVGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVS 62

Query: 74  QKALSSS------TKLHLEQLSDTLYTR-PSLLSELSKFTVNELRRLKVKPFLLRSSAAA 133
           ++   S       +K+H +++SDTLY+R PSLL+ LSK  V  ++    K  ++R +   
Sbjct: 63  ERGGYSKVVSLTRSKIHFDEISDTLYSRGPSLLTRLSKL-VRSVKCFNYKGLIMRGNVVE 122

Query: 134 PVG-------------STFSVNSTIVEMLQEKINGGQSPRWSECNCKLCTCW-SSPSKQS 193
                              ++NST++E          +PRWS+C+C  CT W +S ++ S
Sbjct: 123 SCDHHESKKKISKGKKRLMTLNSTVIE------KSSTAPRWSDCHCSFCTSWLTSTNRDS 182

Query: 194 LFVRSQGPRD--QPREDVLFIHGFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRS 253
           LFV+ Q P+D  + R++V+FIHGF+SSSAFWTETLFPNFS SAKS YR +AVDLLG+GRS
Sbjct: 183 LFVKVQQPKDNKKARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRS 242

Query: 254 PKPADSLYTLKEHVDMIETSVLEAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLA 313
           PKP DSLYTL+EH++MIE SV+  +K+K+FHIVAHSLGCILALALAVKHPG++KSLTLLA
Sbjct: 243 PKPNDSLYTLREHLEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLA 302

Query: 314 PPYYPVPKGEEASQYVMRKVAPRRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFL 373
           PPYY VPKG + +QYVMR+VA + VWPP+  GAS+  WYEH+ RT+ L++ KNH+  EF+
Sbjct: 303 PPYYKVPKGVQPAQYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFV 362

Query: 374 TKLVTRNRIESFLVEGFFAHTHNAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHG 433
           T+L+T NR+ ++L+EGF  HTHN ++HTLHN+I G+G K++ YLD +R+ V+C V IFHG
Sbjct: 363 TRLLTLNRMRTYLIEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHG 422

Query: 434 GDDDVIPLECSYNVKARVPRARVNVVQNKDHITIVVGRQKAFARELEQIWSNSNAS 466
           G D++IP+ECSY+VK++VPRA V+V+ +KDHITIVVGRQK FARELE IW  + ++
Sbjct: 423 GKDELIPVECSYSVKSKVPRATVHVIPDKDHITIVVGRQKDFARELELIWQRTKST 471

BLAST of Lcy02g012520 vs. TAIR 10
Match: AT5G17780.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.5e-88
Identity = 177/446 (39.69%), Postives = 256/446 (57.40%), Query Frame = 0

Query: 21  ALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASSKILVSQKALSSST 80
           ALSF +F  LDLLD +LC +Y+  D   E     CYC++               A  S T
Sbjct: 14  ALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTA--------------AAPQSQT 73

Query: 81  KLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVK-PFLLRSSAAAPVGSTFSVNSTIVE 140
               E  S+TL+ R ++   +  + +   R  K K    LR S                +
Sbjct: 74  TDENELSSETLFGRRNIFRGM--WFLGFAREFKSKLSRKLRKS----------------K 133

Query: 141 MLQEKINGGQSPRWSECNCKLCTCWSSPSKQSL--FVRSQGPRD----QPREDVLFIHGF 200
           + QE +N     RWS+C CK C  W+     +L   V+    R+    +P E+V+FIHGF
Sbjct: 134 IHQESVN-----RWSDCGCKSCKSWTKNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGF 193

Query: 201 ISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEHVDMIETSVLEA 260
           + SS FWTET+F +     K  YRLLA+DLLGFG SPKP DSLYTLK+HVD IE SV++ 
Sbjct: 194 MGSSHFWTETVFEHIQ---KDDYRLLAIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKP 253

Query: 261 YKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEASQYVMRKVAPRR 320
           Y++ SFH+VAHS+GC++ALALA KH   VKS+TL+APPY+P          V+ ++A +R
Sbjct: 254 YQLDSFHVVAHSMGCLIALALAAKHSNIVKSVTLVAPPYFP----SSVEGSVLNRIARKR 313

Query: 321 VWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFLVEGFFAHTHNA 380
           +WPP+A G ++  WYEHI R VC +ICK+H+ WE+L KL    R   + ++    HTH++
Sbjct: 314 LWPPLAFGTAVMSWYEHIGRCVCFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHS 373

Query: 381 AWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYNVKARVPRARVN 440
           AWH++HNVICG     + +L+ +  +   ++++  G  D ++P  CS N+K   P   V+
Sbjct: 374 AWHSMHNVICGGSKVADEHLETL-IKSGVKIHLMQGDCDQIVPSHCSGNMKRTFPAVEVD 414

Query: 441 VVQNKDHITIVVGRQKAFARELEQIW 460
           ++   DH +++ GR + FA +LE IW
Sbjct: 434 IITGADHDSMISGRGEEFAEKLESIW 414

BLAST of Lcy02g012520 vs. TAIR 10
Match: AT5G17780.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 322.8 bits (826), Expect = 4.6e-88
Identity = 176/448 (39.29%), Postives = 255/448 (56.92%), Query Frame = 0

Query: 21  ALSFFLFSLLDLLDILLCFLYKLADFFFESEWKPCYCSSHQESFASSKILVSQKALSSST 80
           ALSF +F  LDLLD +LC +Y+  D   E     CYC++               A  S T
Sbjct: 14  ALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTA--------------AAPQSQT 73

Query: 81  KLHLEQLSDTLYTRPSLLSELSKFTVNELRRLKVK-PFLLRSSAAAPVGSTFSVNSTIVE 140
               E  S+TL+ R ++   +  + +   R  K K    LR S                +
Sbjct: 74  TDENELSSETLFGRRNIFRGM--WFLGFAREFKSKLSRKLRKS----------------K 133

Query: 141 MLQEKINGGQSPRWSECNCKLCTCWSSPSKQSLFV--------RSQGPRDQPREDVLFIH 200
           + QE +N     RWS+C CK C  W+     +L V         ++    +P E+V+FIH
Sbjct: 134 IHQESVN-----RWSDCGCKSCKSWTKNEDGNLHVVVKDSTSRETEYSVQEPSENVIFIH 193

Query: 201 GFISSSAFWTETLFPNFSASAKSTYRLLAVDLLGFGRSPKPADSLYTLKEHVDMIETSVL 260
           GF+ SS FWTET+F +     K  YRLLA+DLLGFG SPKP DSLYTLK+HVD IE SV+
Sbjct: 194 GFMGSSHFWTETVFEHIQ---KDDYRLLAIDLLGFGESPKPRDSLYTLKDHVDTIERSVI 253

Query: 261 EAYKVKSFHIVAHSLGCILALALAVKHPGSVKSLTLLAPPYYPVPKGEEASQYVMRKVAP 320
           + Y++ SFH+VAHS+GC++ALALA KH   VKS+TL+APPY+P          V+ ++A 
Sbjct: 254 KPYQLDSFHVVAHSMGCLIALALAAKHSNIVKSVTLVAPPYFP----SSVEGSVLNRIAR 313

Query: 321 RRVWPPIALGASIACWYEHISRTVCLLICKNHRFWEFLTKLVTRNRIESFLVEGFFAHTH 380
           +R+WPP+A G ++  WYEHI R VC +ICK+H+ WE+L KL    R   + ++    HTH
Sbjct: 314 KRLWPPLAFGTAVMSWYEHIGRCVCFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTH 373

Query: 381 NAAWHTLHNVICGTGGKIERYLDAIRERVNCRVNIFHGGDDDVIPLECSYNVKARVPRAR 440
           ++AWH++HNVICG     + +L+ +  +   ++++  G  D ++P  CS N+K   P   
Sbjct: 374 HSAWHSMHNVICGGSKVADEHLETL-IKSGVKIHLMQGDCDQIVPSHCSGNMKRTFPAVE 416

Query: 441 VNVVQNKDHITIVVGRQKAFARELEQIW 460
           V+++   DH +++ GR + FA +LE IW
Sbjct: 434 VDIITGADHDSMISGRGEEFAEKLESIW 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O229772.0e-16059.67Probable lysophospholipase BODYGUARD 3 OS=Arabidopsis thaliana OX=3702 GN=BDG3 P... [more]
Q8LFX71.3e-15659.27Probable lysophospholipase BODYGUARD 1 OS=Arabidopsis thaliana OX=3702 GN=BDG1 P... [more]
Q9FJ241.9e-14754.62Probable lysophospholipase BODYGUARD 2 OS=Arabidopsis thaliana OX=3702 GN=BDG2 P... [more]
Q700D54.9e-8739.69Probable lysophospholipase BODYGUARD 4 OS=Arabidopsis thaliana OX=3702 GN=BDG4 P... [more]
Q9FN792.8e-8236.14Probable lysophospholipase BODYGUARD 5 OS=Arabidopsis thaliana OX=3702 GN=BDG5 P... [more]
Match NameE-valueIdentityDescription
A0A6J1GZT81.7e-23687.72probable lysophospholipase BODYGUARD 3 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1K6F82.1e-23487.28probable lysophospholipase BODYGUARD 3 OS=Cucurbita maxima OX=3661 GN=LOC1114915... [more]
A0A6J1I4D03.4e-22482.68probable lysophospholipase BODYGUARD 1 OS=Cucurbita maxima OX=3661 GN=LOC1114690... [more]
A0A6J1EM175.5e-22281.82probable lysophospholipase BODYGUARD 3 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A5A7TLN81.4e-21781.51Alpha/beta hydrolase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
Match NameE-valueIdentityDescription
XP_022957687.13.6e-23687.72probable lysophospholipase BODYGUARD 3 [Cucurbita moschata][more]
XP_023534416.15.2e-23587.50probable lysophospholipase BODYGUARD 3 [Cucurbita pepo subsp. pepo][more]
KAG7035517.13.4e-23487.07putative lysophospholipase BODYGUARD 3, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022996320.14.4e-23487.28probable lysophospholipase BODYGUARD 3 [Cucurbita maxima][more]
XP_022970039.17.1e-22482.68probable lysophospholipase BODYGUARD 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G24140.11.4e-16159.67alpha/beta-Hydrolases superfamily protein [more]
AT1G64670.19.4e-15859.27alpha/beta-Hydrolases superfamily protein [more]
AT5G41900.11.4e-14854.62alpha/beta-Hydrolases superfamily protein [more]
AT5G17780.13.5e-8839.69alpha/beta-Hydrolases superfamily protein [more]
AT5G17780.24.6e-8839.29alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 261..274
score: 36.45
coord: 215..230
score: 41.38
coord: 275..288
score: 33.99
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 187..299
e-value: 7.3E-21
score: 75.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 164..461
e-value: 8.5E-41
score: 142.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 171..456
NoneNo IPR availablePANTHERPTHR43689HYDROLASEcoord: 1..463
NoneNo IPR availablePANTHERPTHR43689:SF9LYSOPHOSPHOLIPASE BODYGUARD 3-RELATEDcoord: 1..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy02g012520.1Lcy02g012520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity