Lcy02g012420 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy02g012420
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	Chr02: 37369347 .. 37372836 (+)
RNA-Seq Expression	Lcy02g012420
Synteny	Lcy02g012420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACAACCCGCACGGCTCCTCCCCTTACTTCCGCCGCACACTCTACTCTCTGTCCACACTCTGACCAGACGCCGCCCGGTGGCTCACCGTCGTATCCTCCTGAAGCCGTCTCCCACGACGGCGTTCCTGTACTACCTCCAGATCGCTCCTCACTCTCACTCTTCAGTGCAGTTATTGGCATTTTCGGACTTCGAATTTCACTTTTAGGCTACTGAAGTTTCAAGCTCCATGGAGCTGAGAAGGTTCGGATTCGAGCAATGCGGTTCGTTCGTTCTACTTCTGGTTTCAGGTGATCTGGAATTTCATGAACTTGTGCTTTTTTTTGTTGGTTTAGGATGTGGATTTTTGTGAAATTTTAGAGTGTTGTTCAGTTGGAATTCTCTTGAACTTGTTGTTTTGTGAAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAGATAGCGAACAATCTTCCTGCTCCGGAGAATGTAATCCCGTTGGTGAAAGCGATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACGAGTGTGGAGTTCATAGTCGGACTTGGAAACGAATACTTGGCGAAAATGAAGAATCCGGCTTGTGCTCAAGAGTGGGTGAAGAACAATGTTCAAGCGTACTTGCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTCTTGACCTTCAATGATACTTCTCTTAGTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTGAATCTAGGGCTAGATAAGCAGGTGGTCGTGACGACGGCGCATTCCTTGGCCATACTTGAAACCTCTTATCCGCCTTCTGCCGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTGATCCCAATCTTGGACTTCCATGTCAAGATCGGCTCCCCGTTCTTAATCAATGCGTACCCTTACTTCGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTTGATTTCGTCCTCTTCCAGCCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGATGCGGTTTACTATGCGCTCGCCGCTGTTGGTTACAAGAAACTTCCTGTTCACATCTCGGAGACTGGATGGCCGTCGAAGGGAGACGAAGATGAGGCCGGTGCGACTCCGGAGAATGCGAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGGACGCCATTAAGGCCAAGCTCGGACCTCAACATTTATGTGTTTGCTTTGTTTAACGAGAATATGAAGCCTGGACCAACTTCAGAGAGGAATTATGGGCTCTTTAAACCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGGAACGGTACTCATGGCACTTCCGGCGTGCAACCTTCGCCGCCGGGATCTCCCACCACCTCCACTGGTTATTTGTCCATTTCCTCCGCCACGGTAAAATTTTCCTATTACCCTTATTGTCTTTAATGATTATTGTTATCTTTTTTGCTTGATTTGCGTTCGGTTCATAAAGTGACAAAGGTAGAAACAAGTATAGGGTTAAACTAAGACTTTCTTCCAATCTGTTGAGTGAAAAGAGTTTATTTTTCAATCTCTCCCGATAATTCGGGGGAGATTGAAAATTTCTAATCACTGGAAATTGAGAAAATGGGAATATGAATTGGAAAGAGTTGAGTGGGGGCCATCTTGGTGATTGATCATTGGTGGGAGCACATGGATGCATTAGCCCAATCGCAACTCTTTGGATTCTCGTCAGTTAATTTTGAGGCCATTCTAACATTATAAAGTTGCAGCCATATGCATATCCCCATTCCCTGTGTCGTGTCCTGAACAGAATTCGTCTTTATCTTTATCTTTGTCCTTGGCAGCACAAGAGAAATGTGTAATTCAGTAGTTTTTCTAACTTCTTTTTGTTAAGAAACATTATAATTTCTTTGGGAGCTCAAGCTTTCAAGCCAAGTACTCGAGTAGGTTGTAAAGTGAGCTCAAGTTCAAACTTCTCTTGCAGGATCCTTTATGCAATGGAATGCCATTGTGGAGATGCCCATTTGTCTTAAGGGCTTAAATTTTTTCCCCCTTGTTGAATATAGAAAACTTACTAGCAACTAAATATTTAAACGAAATTAAACGGTAGGACAAATTTGAGTAAAATTCTTTCACATAAAATCATTAATGCCTCTGTTTGCCTGAGTGTTTGTGGAGACTAAGAAACAGTGGTGATGTTTTGGTGGCAGGAGAGAAGCCATTGGCTTGGATTCTTCTCAGCGTGCCTGTTTCTGATGCTGTTCAAGTTGCTACTTTGAATTTAGGAGATTATTTGTTTTTCAGTGTATGTTTCCGAAAGATGCTTTGGATTTATTTCATTTATCAGAATCAGCCGTTAAAAAGCAAATTGGAAGATGCTAAAGAGAATAAGAGGCAGCGTCGGATTTGGGAAAAGGTAAAAATTCTGTTCCTTTCCTTGTACGCGACTACGTTCTCTTTTATTTCTCTGCCTTCCTTTTTGCCATTCTTTTGTGTGTGACCATAACAAAAGTTATTCGGCTTCTGCTTTTCTGCTAACAAAAGGTGCAAATTATGAATGGAAACCATTTTCTGTAATTTCTAAAACAGAATACTGAGCGAAAGACAAATCTTCAATCTTGTTCAATTTTTAAGTGAACAATGCCCCTATTGGCCGTTTACTCATATCCTCTTTGGCAATAATCATGAAAATCTGGAAAAAGGATAGAAAAAAGAACCCTATTCGCCACACTTTTTTTCTTCTTCTCAGTTGAGTGCTTTTGTGATATTTTGAACTTTTGCTGTACTCGATATCTTTGCTTTCATACCTCTTATATTTTCAGGGTTGTTTCTATATTCTTTTCTTGGTTATTTAAATGAAGATTTTTTTTTTAATGAATAAAAATGAAGATTTGAACATGCAAAACTTTTTGTAAGGATTATTAGGTGCTAATTAATAGCTAAACATTTTACTAAGAAAAAGTAGAAATATCATCGAAAGTTTCTTGAATTTACCACTAGTCACTTAATGAAGTGGGGCGAACAACTGACAGAGCCATGTGACAAAAGAAAAAGTTCAGTTTGGAATTTTTATCATTTCCTTTTTTGTATGCATTCCATTTGATATTCTTGTCTTAGCTGGATCTGATCTGTTAATATCTCTTCTTTTTTTTTTTATCCTGCTTTTTACAGGGATATATCAGTGTTTTAGTAATTAATAATTTTTATGTGAATTTGGTTCGTCGATGGACATAAAATCTGGTGGATGGTTGATCCGAACTTCTTCTTTGTAATGCTATTTAGTATGGAAATTTTATTAATTTGCCTTTTTGATGTACCTCTCTCCTCTATTCTCCACGTTTGATTCTAGTTCGACTTTTATTATATTCAGAGATTTATTGGATTTTTCGTTTTTTT

mRNA sequence

CCACAACCCGCACGGCTCCTCCCCTTACTTCCGCCGCACACTCTACTCTCTGTCCACACTCTGACCAGACGCCGCCCGGTGGCTCACCGTCGTATCCTCCTGAAGCCGTCTCCCACGACGGCGTTCCTGTACTACCTCCAGATCGCTCCTCACTCTCACTCTTCAGTGCAGTTATTGGCATTTTCGGACTTCGAATTTCACTTTTAGGCTACTGAAGTTTCAAGCTCCATGGAGCTGAGAAGGTTCGGATTCGAGCAATGCGGTTCGTTCGTTCTACTTCTGGTTTCAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAGATAGCGAACAATCTTCCTGCTCCGGAGAATGTAATCCCGTTGGTGAAAGCGATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACGAGTGTGGAGTTCATAGTCGGACTTGGAAACGAATACTTGGCGAAAATGAAGAATCCGGCTTGTGCTCAAGAGTGGGTGAAGAACAATGTTCAAGCGTACTTGCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTCTTGACCTTCAATGATACTTCTCTTAGTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTGAATCTAGGGCTAGATAAGCAGGTGGTCGTGACGACGGCGCATTCCTTGGCCATACTTGAAACCTCTTATCCGCCTTCTGCCGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTGATCCCAATCTTGGACTTCCATGTCAAGATCGGCTCCCCGTTCTTAATCAATGCGTACCCTTACTTCGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTTGATTTCGTCCTCTTCCAGCCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGATGCGGTTTACTATGCGCTCGCCGCTGTTGGTTACAAGAAACTTCCTGTTCACATCTCGGAGACTGGATGGCCGTCGAAGGGAGACGAAGATGAGGCCGGTGCGACTCCGGAGAATGCGAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGGACGCCATTAAGGCCAAGCTCGGACCTCAACATTTATGTGTTTGCTTTGTTTAACGAGAATATGAAGCCTGGACCAACTTCAGAGAGGAATTATGGGCTCTTTAAACCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGGAACGGTACTCATGGCACTTCCGGCGTGCAACCTTCGCCGCCGGGATCTCCCACCACCTCCACTGGTTATTTGTCCATTTCCTCCGCCACGGAGAGAAGCCATTGGCTTGGATTCTTCTCAGCGTGCCTGTTTCTGATGCTGTTCAAGTTGCTACTTTGAATTTAGGAGATTATTTGTTTTTCAGTGTATGTTTCCGAAAGATGCTTTGGATTTATTTCATTTATCAGAATCAGCCGTTAAAAAGCAAATTGGAAGATGCTAAAGAGAATAAGAGGCAGCGTCGGATTTGGGAAAAGGGATATATCAGTGTTTTAGTAATTAATAATTTTTATGTGAATTTGGTTCGTCGATGGACATAAAATCTGGTGGATGGTTGATCCGAACTTCTTCTTTGTAATGCTATTTAGTATGGAAATTTTATTAATTTGCCTTTTTGATGTACCTCTCTCCTCTATTCTCCACGTTTGATTCTAGTTCGACTTTTATTATATTCAGAGATTTATTGGATTTTTCGTTTTTTT

Coding sequence (CDS)

ATGGAGCTGAGAAGGTTCGGATTCGAGCAATGCGGTTCGTTCGTTCTACTTCTGGTTTCAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAGATAGCGAACAATCTTCCTGCTCCGGAGAATGTAATCCCGTTGGTGAAAGCGATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACGAGTGTGGAGTTCATAGTCGGACTTGGAAACGAATACTTGGCGAAAATGAAGAATCCGGCTTGTGCTCAAGAGTGGGTGAAGAACAATGTTCAAGCGTACTTGCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTCTTGACCTTCAATGATACTTCTCTTAGTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTGAATCTAGGGCTAGATAAGCAGGTGGTCGTGACGACGGCGCATTCCTTGGCCATACTTGAAACCTCTTATCCGCCTTCTGCCGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTGATCCCAATCTTGGACTTCCATGTCAAGATCGGCTCCCCGTTCTTAATCAATGCGTACCCTTACTTCGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTTGATTTCGTCCTCTTCCAGCCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGATGCGGTTTACTATGCGCTCGCCGCTGTTGGTTACAAGAAACTTCCTGTTCACATCTCGGAGACTGGATGGCCGTCGAAGGGAGACGAAGATGAGGCCGGTGCGACTCCGGAGAATGCGAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGGACGCCATTAAGGCCAAGCTCGGACCTCAACATTTATGTGTTTGCTTTGTTTAACGAGAATATGAAGCCTGGACCAACTTCAGAGAGGAATTATGGGCTCTTTAAACCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGGAACGGTACTCATGGCACTTCCGGCGTGCAACCTTCGCCGCCGGGATCTCCCACCACCTCCACTGGTTATTTGTCCATTTCCTCCGCCACGGAGAGAAGCCATTGGCTTGGATTCTTCTCAGCGTGCCTGTTTCTGATGCTGTTCAAGTTGCTACTTTGA

Protein sequence

MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSGVQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL

Homology

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BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 6.2e-142
Identity = 253/403 (62.78%), Postives = 313/403 (77.67%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MEL  F      S + L+   L++LP   +S+GVNYGQI +NLP+P +VIPL+K+IGATK
Sbjct: 1   MELTSF---HRSSLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDA+P++L+AF+NT +EFI+GLGNEYL+KMK+P+ A  W+K NV  +LP T IT I 
Sbjct: 61  VKLYDANPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCIT 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           +GNE+L  ND+SL+ NLLPAMQ VH+AL+  GL  Q+ VTTAHSL+IL++S+PPSAG F+
Sbjct: 121 IGNEILALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQ 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
            DL+D L PIL+FH K  SPFLINAYP+FAYK NPK+V LDFVLFQPNQG++DP +  HY
Sbjct: 181 PDLLDSLTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVY ALAA G+K L V ISETGWPSKGD+DE GATPENAK+YNGNL+K + 
Sbjct: 241 DNMLFAQIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMM 300

Query: 301 --QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAV-- 360
             +K  TPL+P++DL+IYVFALFNEN+KPGPTSERNYGLFKPDGT  Y LGF++ND V  
Sbjct: 301 SGKKTKTPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRG 360

Query: 361 --GNGTHG----------TSGVQPSPPGSP-TTSTGYLSISSA 387
             G GT G           S V P  P +P + STGYL+IS++
Sbjct: 361 ASGGGTGGGNSSSGGGRDKSPVFPVSPVAPDSASTGYLAISAS 400

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BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.1e-137
Identity = 257/413 (62.23%), Postives = 302/413 (73.12%), Query Frame = 0


Query: 13  SFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+K++GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  AQ WVK NVQAYLP TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILD 192
           L+A L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAVG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL AVG  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF--SINDAVGNGTHGTSGVQ 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   S   + G+G+  ++G  
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PSPPGSPT----------------TSTGYLSISSATERSHW----LGFFSACL 401
            S  G  T                 S  Y+SISSA  +  +    L FF  C+
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGGKGRFVECVLFFFLLCI 420

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BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-116
Identity = 202/336 (60.12%), Postives = 261/336 (77.68%), Query Frame = 0


Query: 15  VLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++++  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  AQ+W++  ++ ++  T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 SANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

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BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.5e-83
Identity = 155/349 (44.41%), Postives = 233/349 (66.76%), Query Frame = 0


Query: 28  MVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAFANTSVEFIVGLGN 87
           M S +G+ YG+ A+NLP+P  V  L++ +    V++YDA+  VL+AFANT +E ++G+ N
Sbjct: 23  MASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPN 82

Query: 88  -EYLAKMKNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSLSANLLPAMQSVHT 147
            + LA  +  +    W+ NN+  Y P TKITSI VG EV    D + +  +LPAM+++HT
Sbjct: 83  ADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA-TGLVLPAMRNIHT 142

Query: 148 ALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDFHVKIGSPFLINAY 207
           AL   GLDK++ ++++HSLAIL  S+PPS+  F +     L P+L+F V+  SPF+I+ Y
Sbjct: 143 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLY 202

Query: 208 PYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAVGYKKLPVH 267
           PY+AY+ + ++V L++ LF+ +  V+DP + L Y NM  AQ+DA+Y+AL A+ +K + V 
Sbjct: 203 PYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVM 262

Query: 268 ISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMK 327
           ++E+GWPSKG   E  ATPENA  YN NL++ +    GTP +P  ++++Y+F+LFNEN K
Sbjct: 263 VTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRK 322

Query: 328 PGPTSERNYGLFKPDGTPVYQLGFS-INDAVGNGTHGTSGVQPSPPGSP 375
           PG  SERN+G+F  +GT VY L F+  N    + T+ T+G  PSP  SP
Sbjct: 323 PGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSP 370

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BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 6.7e-80
Identity = 152/347 (43.80%), Postives = 219/347 (63.11%), Query Frame = 0


Query: 30  SSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAFANTSVEFIVGLGNEY 89
           S +GVNYGQ+++NLP+ +  + L+K+    KV+L+ A P V++AFANT VE ++G  N  
Sbjct: 28  SFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGD 87

Query: 90  LAKM-KNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSLSANLLPAMQSVHTAL 149
           +  +  NP  A ++VK+NV ++ P + I +I VGNEVLT  D  L + LLPAMQ+V  AL
Sbjct: 88  IPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNAL 147

Query: 150 VNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDFHVKIGSPFLINAYPY 209
               L  +V V+T H++A+L  SYPPS+GVF   L D +  +L F     +PF+I+ YPY
Sbjct: 148 NAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMISPYPY 207

Query: 210 FAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAVGYKKLPVHIS 269
           FAYK  P   +L F LFQPN G +D G+   Y NM  AQ+DAV+ AL A+G+K + + ++
Sbjct: 208 FAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVA 267

Query: 270 ETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMKPG 329
           ETGWP  GD +E G + +NAK Y GNL+  +  K GTPL P   ++ Y+F+L++E+ K G
Sbjct: 268 ETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTG 327

Query: 330 PTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSGVQPSPPGSPT 376
            +SE+ +GLFKPDG+  Y +G   N              P+ P +PT
Sbjct: 328 ASSEKYFGLFKPDGSTTYDVGLLKNTQ-----------NPTTPATPT 363

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BLAST of Lcy02g012420 vs. ExPASy TrEMBL
Match: A0A0A0KK78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G146960 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.1e-225
Identity = 390/409 (95.35%), Postives = 401/409 (98.04%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGF+QCGSFVLLL+SGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSIND VGNGT
Sbjct: 301 QKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDGVGNGT 360

Query: 361 HGTSGVQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTS  QPSPPG PTTSTGYL+ISSATERSHW+GFFS  LFLM+FKLLL
Sbjct: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHWVGFFSMFLFLMVFKLLL 409

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BLAST of Lcy02g012420 vs. ExPASy TrEMBL
Match: A0A6J1H1N2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459311 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 2.7e-225
Identity = 395/412 (95.87%), Postives = 403/412 (97.82%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGSFVLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTSTGYLSISSA ERS  LGFFS CL+LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSRCLGFFSVCLYLMLFKLLL 412

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BLAST of Lcy02g012420 vs. ExPASy TrEMBL
Match: A0A6J1K8L2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491117 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.3e-223
Identity = 393/412 (95.39%), Postives = 401/412 (97.33%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGS VLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGS-VLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLA+LETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAVLETSYPPSAGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTS GYLSISSA ERSH LGFF  CL+LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSNGYLSISSAKERSHCLGFFLVCLYLMLFKLLL 411

[Close]

BLAST of Lcy02g012420 vs. ExPASy TrEMBL
Match: A0A6J1E421 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111025884 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 2.8e-222
Identity = 391/409 (95.60%), Postives = 398/409 (97.31%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRR GFEQC S  LLLVSG LLLPVMVSS+GVNYGQIANNLPAPENVI LVK+IGATK
Sbjct: 1   MELRRPGFEQCCSVFLLLVSG-LLLPVMVSSIGVNYGQIANNLPAPENVISLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQ YLPGT+ITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQDYLPGTRITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPG+NLHY
Sbjct: 181 RDLVDCLTPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGTNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVY ALAAVGYKKLPVHISETGWPSKGDEDEAGATP+NAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYSALAAVGYKKLPVHISETGWPSKGDEDEAGATPDNAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF+INDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFTINDAVGNGT 360

Query: 361 HGTSGVQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG QPSPPGSPTTSTGYLSISSATERSHWLGFFSAC+FLMLFKLLL
Sbjct: 361 HGTSGTQPSPPGSPTTSTGYLSISSATERSHWLGFFSACMFLMLFKLLL 408

[Close]

BLAST of Lcy02g012420 vs. ExPASy TrEMBL
Match: A0A5A7TM33 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001430 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.4e-221
Identity = 385/410 (93.90%), Postives = 399/410 (97.32%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSG-LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGAT 60
           MELRRFGF+QCGS VLLL+SG LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVK+IGAT
Sbjct: 1   MELRRFGFKQCGSLVLLLISGLLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60

Query: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSI 120
           KVKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACA+EWVKNNVQAY PGTKITSI
Sbjct: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSI 120

Query: 121 FVGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVF 180
           FVGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPSAGVF
Sbjct: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180

Query: 181 RRDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLH 240
           RRDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNL 
Sbjct: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLR 240

Query: 241 YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFI 300
           YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGD+DEAGATPENAKKYNGNLLKFI
Sbjct: 241 YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDDDEAGATPENAKKYNGNLLKFI 300

Query: 301 CQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNG 360
           CQKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF+IND VGNG
Sbjct: 301 CQKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFTINDGVGNG 360

Query: 361 THGTSGVQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           THGTS  QPSPPG+PTTSTGYL+ISSATERSHW+GFFS  LFL +FKLLL
Sbjct: 361 THGTSSAQPSPPGTPTTSTGYLAISSATERSHWVGFFSMSLFLTVFKLLL 410

[Close]

BLAST of Lcy02g012420 vs. NCBI nr
Match: XP_023534433.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 793.9 bits (2049), Expect = 6.6e-226
Identity = 396/412 (96.12%), Postives = 404/412 (98.06%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGSFVLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTSTGYLSISSA ERSH LGFFS CL+LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSHCLGFFSVCLYLMLFKLLL 412

[Close]

BLAST of Lcy02g012420 vs. NCBI nr
Match: XP_011654675.1 (glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN49973.1 hypothetical protein Csa_000456 [Cucumis sativus])

HSP 1 Score: 791.2 bits (2042), Expect = 4.3e-225
Identity = 390/409 (95.35%), Postives = 401/409 (98.04%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGF+QCGSFVLLL+SGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSIND VGNGT
Sbjct: 301 QKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDGVGNGT 360

Query: 361 HGTSGVQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTS  QPSPPG PTTSTGYL+ISSATERSHW+GFFS  LFLM+FKLLL
Sbjct: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHWVGFFSMFLFLMVFKLLL 409

[Close]

BLAST of Lcy02g012420 vs. NCBI nr
Match: XP_022957923.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata])

HSP 1 Score: 790.8 bits (2041), Expect = 5.6e-225
Identity = 395/412 (95.87%), Postives = 403/412 (97.82%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGSFVLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTSTGYLSISSA ERS  LGFFS CL+LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSRCLGFFSVCLYLMLFKLLL 412

[Close]

BLAST of Lcy02g012420 vs. NCBI nr
Match: KAG6605611.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 790.4 bits (2040), Expect = 7.3e-225
Identity = 395/412 (95.87%), Postives = 404/412 (98.06%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGSFVLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMK+PACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKSPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNL+KFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLIKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTSTGYLSISSA ERSH LGFFSACL LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSHRLGFFSACLSLMLFKLLL 412

[Close]

BLAST of Lcy02g012420 vs. NCBI nr
Match: XP_022995648.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima])

HSP 1 Score: 783.9 bits (2023), Expect = 6.9e-223
Identity = 393/412 (95.39%), Postives = 401/412 (97.33%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MELRRFGFEQCGS VLLLVSGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVKAIGATK
Sbjct: 1   MELRRFGFEQCGS-VLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQV VTTAHSLA+LETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAVLETSYPPSAGVFR 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCL+PILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSG---VQPSPPGSPTTSTGYLSISSATERSHWLGFFSACLFLMLFKLLL 410
           HGTSG   VQP+PPG PTTS GYLSISSA ERSH LGFF  CL+LMLFKLLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSNGYLSISSAKERSHCLGFFLVCLYLMLFKLLL 411

[Close]

BLAST of Lcy02g012420 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 505.4 bits (1300), Expect = 4.4e-143
Identity = 253/403 (62.78%), Postives = 313/403 (77.67%), Query Frame = 0


Query: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATK 60
           MEL  F      S + L+   L++LP   +S+GVNYGQI +NLP+P +VIPL+K+IGATK
Sbjct: 1   MELTSF---HRSSLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120
           VKLYDA+P++L+AF+NT +EFI+GLGNEYL+KMK+P+ A  W+K NV  +LP T IT I 
Sbjct: 61  VKLYDANPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCIT 120

Query: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFR 180
           +GNE+L  ND+SL+ NLLPAMQ VH+AL+  GL  Q+ VTTAHSL+IL++S+PPSAG F+
Sbjct: 121 IGNEILALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQ 180

Query: 181 RDLVDCLIPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
            DL+D L PIL+FH K  SPFLINAYP+FAYK NPK+V LDFVLFQPNQG++DP +  HY
Sbjct: 181 PDLLDSLTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHY 240

Query: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVY ALAA G+K L V ISETGWPSKGD+DE GATPENAK+YNGNL+K + 
Sbjct: 241 DNMLFAQIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMM 300

Query: 301 --QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAV-- 360
             +K  TPL+P++DL+IYVFALFNEN+KPGPTSERNYGLFKPDGT  Y LGF++ND V  
Sbjct: 301 SGKKTKTPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRG 360

Query: 361 --GNGTHG----------TSGVQPSPPGSP-TTSTGYLSISSA 387
             G GT G           S V P  P +P + STGYL+IS++
Sbjct: 361 ASGGGTGGGNSSSGGGRDKSPVFPVSPVAPDSASTGYLAISAS 400

[Close]

BLAST of Lcy02g012420 vs. TAIR 10
Match: AT5G42100.1 (beta-1,3-glucanase_putative )

HSP 1 Score: 490.3 bits (1261), Expect = 1.5e-138
Identity = 257/413 (62.23%), Postives = 302/413 (73.12%), Query Frame = 0


Query: 13  SFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+K++GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  AQ WVK NVQAYLP TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILD 192
           L+A L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAVG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL AVG  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF--SINDAVGNGTHGTSGVQ 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   S   + G+G+  ++G  
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PSPPGSPT----------------TSTGYLSISSATERSHW----LGFFSACL 401
            S  G  T                 S  Y+SISSA  +  +    L FF  C+
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGGKGRFVECVLFFFLLCI 420

[Close]

BLAST of Lcy02g012420 vs. TAIR 10
Match: AT5G42100.2 (beta-1,3-glucanase_putative )

HSP 1 Score: 488.0 bits (1255), Expect = 7.2e-138
Identity = 258/414 (62.32%), Postives = 301/414 (72.71%), Query Frame = 0


Query: 13  SFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+K++GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  AQ WVK NVQAYLP TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LSANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILD 192
           L+A L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAVG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL AVG  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF--SINDAVGNGTHGTSGVQ 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   S   + G+G+  ++G  
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PSPPGSPT----------------TSTGYLSISSATERSHWLGFFSACLFLMLF 406
            S  G  T                 S  Y+SISSA      L F   C  L +F
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGVTFSTLHFI-CCFTLTIF 420

[Close]

BLAST of Lcy02g012420 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 8.3e-118
Identity = 202/336 (60.12%), Postives = 261/336 (77.68%), Query Frame = 0


Query: 15  VLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++++  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  AQ+W++  ++ ++  T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 SANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

[Close]

BLAST of Lcy02g012420 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 8.3e-118
Identity = 202/336 (60.12%), Postives = 261/336 (77.68%), Query Frame = 0


Query: 15  VLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKAIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++++  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAQEWVKNNVQAYLPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  AQ+W++  ++ ++  T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 SANLLPAMQSVHTALVNLGLDKQVVVTTAHSLAILETSYPPSAGVFRRDLVDCLIPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

The following BLAST results are available for this feature:

BLAST of Lcy02g012420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L868	6.2e-142	62.78	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	2.1e-137	62.23	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9ZQG9	1.2e-116	60.12	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8VYE5	6.5e-83	44.41	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q94G86	6.7e-80	43.80	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]

BLAST of Lcy02g012420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK78	2.1e-225	95.35	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G146960 PE=3...	[more]
A0A6J1H1N2	2.7e-225	95.87	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459311 P...	[more]
A0A6J1K8L2	3.3e-223	95.39	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491117 PE=...	[more]
A0A6J1E421	2.8e-222	95.60	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111025884 ...	[more]
A0A5A7TM33	2.4e-221	93.90	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Lcy02g012420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534433.1	6.6e-226	96.12	glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo]	[more]
XP_011654675.1	4.3e-225	95.35	glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN49973.1 hypothetical p...	[more]
XP_022957923.1	5.6e-225	95.87	glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata]	[more]
KAG6605611.1	7.3e-225	95.87	Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022995648.1	6.9e-223	95.39	glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima]	[more]

BLAST of Lcy02g012420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32860.1	4.4e-143	62.78	Glycosyl hydrolase superfamily protein	[more]
AT5G42100.1	1.5e-138	62.23	beta-1,3-glucanase_putative	[more]
AT5G42100.2	7.2e-138	62.32	beta-1,3-glucanase_putative	[more]
AT2G27500.1	8.3e-118	60.12	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	8.3e-118	60.12	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 32..350 e-value: 2.3E-86 score: 290.2
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 30..357 e-value: 2.7E-123 score: 413.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 359..380
None	No IPR available	PANTHER	PTHR32227:SF348	GLYCOSIDE HYDROLASE FAMILY 17-RELATED	coord: 16..400
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 16..400
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 32..349

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy02g012420.1	Lcy02g012420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds