Lcy02g012250 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g012250 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 537.7 bits (1384), Expect = 1.3e-151
Identity = 304/419 (72.55%), Postives = 338/419 (80.67%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           ME  +  H HI+EV          IV SK+CGEAP CGFSD KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGH+HG  HSHGH HGHG            HD H H HG++VTT HHHHH HE   +   
Sbjct: 181 LGHDHG--HSHGHGHGHG------------HDHHNHSHGVTVTT-HHHHHDHEHGHSHGH 240

Query: 289 GGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVMIG 348
           G   HHAH + VT  LL    D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIG
Sbjct: 241 GEDKHHAHGD-VTEQLL----DKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIG 300

Query: 349 GAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 408
           GAIIWY PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM
Sbjct: 301 GAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEM 360

Query: 409 DEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIER 468
           +EVVA+HELHIWAITVGKVLLACHV ++PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Lcy02g012250 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 519.2 bits (1336), Expect = 4.7e-146
Identity = 296/422 (70.14%), Postives = 339/422 (80.33%), Query Frame = 0

Query: 48  EMEVQNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFSDVKNSSKDAKERSASMRK 107
           +M+   H+H H++++CG+V +   S+VG  K CGEAP CGFSD K SS +A+ER+ASMRK
Sbjct: 15  KMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRK 74

Query: 108 LFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQS 167
           L IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QS
Sbjct: 75  LLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQS 134

Query: 168 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMA 227
           YGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA
Sbjct: 135 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMA 194

Query: 228 LLLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAA 287
           +LLGH+HG  H HGHS     HD+GHG   HSHD   H HGI+ T HHH   H E + + 
Sbjct: 195 ILLGHDHG--HGHGHS-----HDNGHG---HSHD---HGHGIAATEHHHDSGHDESQLSD 254

Query: 288 VDGGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVM 347
           V                L+E             QKKQRN+N+QGAYLHVLGDSIQS+GVM
Sbjct: 255 V----------------LIE-------------QKKQRNVNIQGAYLHVLGDSIQSVGVM 314

Query: 348 IGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLC 407
           IGGAIIWYKPE+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+C
Sbjct: 315 IGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVC 374

Query: 408 EMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIE 467
           E++EVVA+HELHIWAITVGK+LLACHV ++PEA ADMVLDK+I+YI+RE+NISHVTIQIE
Sbjct: 375 EIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 393

Query: 468 RQ 469
           RQ
Sbjct: 435 RQ 393

BLAST of Lcy02g012250 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 9.8e-144
Identity = 294/426 (69.01%), Postives = 330/426 (77.46%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           M+  N     I EV  D+ +      G+K+C  A +C FSD  NSSKDA+ER ASMRKL 
Sbjct: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGA-ACDFSDSSNSSKDARERMASMRKLI 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IAV+LCI+FM+VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATP+QSYG
Sbjct: 61  IAVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQ+IWLLAGILVYEAIVRLIN  GEV+G LMFAVSAFGL VNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGH+HG+ H HGH HGH  HDH HGG +H H    HHH      H H HHH +  G ++ 
Sbjct: 181 LGHDHGHGHGHGHGHGH-SHDHDHGGSDHDH----HHH--EDQEHGHVHHHEDGHGNSIT 240

Query: 289 GGKHHHA----HEESVTAPLLEN--CCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQS 348
              HHH     H      PLL++   CD         +K +RNINV  AYLHVLGDSIQS
Sbjct: 241 VNLHHHPGTGHHHHDAEEPLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQS 300

Query: 349 IGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLE 408
           IGVMIGGAIIWYKPE+KI+DLICTLIFS IVL TTI+MLRNILEVLMESTPREIDAT LE
Sbjct: 301 IGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLE 360

Query: 409 KGLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVT 468
            GL +MD VVA+HELHIWAITVGKVLLACHV +  +A+AD +LDKVI YI+ EYNISHVT
Sbjct: 361 NGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVT 418

BLAST of Lcy02g012250 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.4e-113
Identity = 235/393 (59.80%), Postives = 277/393 (70.48%), Query Frame = 0

Query: 76  SKICGEAPSCGFSDVKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 135
           SK+CGE  +CGFS    SS DAK+R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGFS---TSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 136 DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 195
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 196 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGYAHSHGHSHGHGDHDHGHGG 255
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HG+ H HGHSH   DH H +G 
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSH---DHGHSYG- 185

Query: 256 HEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVDGGKHHHAHEESVTAPLLENCCDDDSQ 315
                                                                   + ++
Sbjct: 186 --------------------------------------------------------ERAE 245

Query: 316 KVTKTQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVL 375
           ++ +  K+ RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FS IVL
Sbjct: 246 QLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVL 305

Query: 376 CTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVI 435
            TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV 
Sbjct: 306 GTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVK 334

Query: 436 VKPEANADMVLDKVIEYIRREYNISHVTIQIER 468
           V+PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 VRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334

BLAST of Lcy02g012250 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 9.1e-81
Identity = 167/385 (43.38%), Postives = 240/385 (62.34%), Query Frame = 0

Query: 84  SCGFSDVKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSD 143
           SC F+  ++   + KER  S R+L   + L ++ MSV++VGG KANSLA++TDAAHLLSD
Sbjct: 35  SCAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSD 94

Query: 144 VAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGP 203
           VA   +SL ++  S WEA PR S+GF R+E+L A +S+Q+IWL++G++++EAI RL++  
Sbjct: 95  VAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRS 154

Query: 204 GEVKGLLMFAVSAFGLVVNIAMALLLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHR 263
            EV G +MF +SAFG  +N+ M L LGH H +                     H HD H 
Sbjct: 155 REVNGEIMFGISAFGFFMNLVMVLWLGHNHSH---------------------HHHDHHH 214

Query: 264 HHHGISVTTHHHHHHHHEEEGAAVDGGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQ 323
           HHH      H H H HH +E  A +        EE    PL          K  K+  K+
Sbjct: 215 HHH-----NHKHQHQHHHKEVVAEE--------EEEEMNPL----------KGEKSSSKE 274

Query: 324 RNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRN 383
            NIN+QGAYLH + D IQS+GVMIGG IIW KP++ ++DLICTL+FSA  L  T+ +L+N
Sbjct: 275 MNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPILKN 334

Query: 384 ILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADM 443
           I  +LME  PR++D  KLE+GL  +D V  +++LH+W ITVG+++L+CH++ +P A+   
Sbjct: 335 IFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGASPKE 375

Query: 444 VLDKVIEYIRREYNISHVTIQIERQ 469
           ++  V  + R+ Y I H T+Q+E +
Sbjct: 395 IITGVRNFCRKSYGIYHATVQVESE 375

BLAST of Lcy02g012250 vs. ExPASy TrEMBL
Match: A0A6J1H2S0 (metal tolerance protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459497 PE=4 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.2e-204
Identity = 383/423 (90.54%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQN DHGH+IEVCGDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGHSHGHGDHDHGHG HEH H+DHRHHHGISVT HHHHH HHEEEGAA D
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 289 GGK---HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 348
           G K   HHHAHEESVT PLLE+     SQKVT TQK++RNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 GVKHHHHHHAHEESVTTPLLES----HSQKVTNTQKRERNINVQGAYLHVLGDSIQSIGV 300

Query: 349 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 408
           MIGGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 409 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 468
           CEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISH+TIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQI 419

BLAST of Lcy02g012250 vs. ExPASy TrEMBL
Match: A0A6J1JZI0 (metal tolerance protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 5.7e-203
Identity = 381/423 (90.07%), Postives = 398/423 (94.09%), Query Frame = 0

Query: 48  EMEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKL 107
           +MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKL
Sbjct: 2   KMEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKL 61

Query: 108 FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 167
           FIA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY
Sbjct: 62  FIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 121

Query: 168 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 227
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL
Sbjct: 122 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 181

Query: 228 LLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAV 287
           LLGHEHG+AHSHGHSHGHGDH+HG GGHEH H+DHRHHHGISVT HHHHH HHEEEGAA 
Sbjct: 182 LLGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAG 241

Query: 288 DGGK--HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 347
           D  K  HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQS+GV
Sbjct: 242 DSVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGV 301

Query: 348 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 407
           M+GGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 302 MVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 361

Query: 408 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 467
           CEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 362 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 420

Query: 468 ERQ 469
           ERQ
Sbjct: 422 ERQ 420

BLAST of Lcy02g012250 vs. ExPASy TrEMBL
Match: A0A6J1JZI6 (metal tolerance protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491116 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 7.4e-203
Identity = 381/422 (90.28%), Postives = 397/422 (94.08%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGHSHGHGDH+HG GGHEH H+DHRHHHGISVT HHHHH HHEEEGAA D
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 289 GGK--HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVM 348
             K  HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQS+GVM
Sbjct: 241 SVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVM 300

Query: 349 IGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLC 408
           +GGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGLC
Sbjct: 301 VGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLC 360

Query: 409 EMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIE 468
           EM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTIQIE
Sbjct: 361 EMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIE 418

BLAST of Lcy02g012250 vs. ExPASy TrEMBL
Match: A0A1S3CB54 (metal tolerance protein 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498976 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 7.7e-200
Identity = 380/424 (89.62%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 48  EMEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKL 107
           +MEVQ  DHGHIIEVCGDVQAVGPSIVGSKICG+APSCGFSD KNSSKDAKERSASMRKL
Sbjct: 7   QMEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKL 66

Query: 108 FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 167
            IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY
Sbjct: 67  CIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 126

Query: 168 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 227
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVS FGLVVNIAMAL
Sbjct: 127 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSTFGLVVNIAMAL 186

Query: 228 LLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAV 287
           LLGHEHG+AHSHGH+HGHG+HDHGHG HEH  +DHRH HGISVT    HHHHH+E+GAA 
Sbjct: 187 LLGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTM---HHHHHDEKGAAS 246

Query: 288 DGGK---HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIG 347
           DG +   HHH H+ES T PLL     D S+KVTKTQKKQRNINVQGAYLHVLGDSIQSIG
Sbjct: 247 DGVEHHHHHHEHKESTTVPLL-----DSSRKVTKTQKKQRNINVQGAYLHVLGDSIQSIG 306

Query: 348 VMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKG 407
           VMIGGAIIWY+PEY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DATKLEKG
Sbjct: 307 VMIGGAIIWYRPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKG 366

Query: 408 LCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQ 467
           LCEM+EVVAIHELHIWAITVGK+LLACHVI+KPEANADMVLDKVIEYIRREYNISHVTIQ
Sbjct: 367 LCEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQ 420

Query: 468 IERQ 469
           IERQ
Sbjct: 427 IERQ 420

BLAST of Lcy02g012250 vs. ExPASy TrEMBL
Match: E5GC92 (Metal tolerance protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.7e-199
Identity = 380/423 (89.83%), Postives = 395/423 (93.38%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQ  DHGHIIEVCGDVQAVGPSIVGSKICG+APSCGFSD KNSSKDAKERSASMRKL 
Sbjct: 1   MEVQ--DHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLC 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVS FGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSTFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGH+HGHG+HDHGHG HEH  +DHRH HGISVT    HHHHH+E+GAA D
Sbjct: 181 LGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTM---HHHHHDEKGAASD 240

Query: 289 GGK---HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 348
           G +   HHH H+ES T PLL     D S+KVTKTQKKQRNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 GVEHHHHHHEHKESTTVPLL-----DSSRKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 300

Query: 349 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 408
           MIGGAIIWY+PEY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DATKLEKGL
Sbjct: 301 MIGGAIIWYRPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKGL 360

Query: 409 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 468
           CEM+EVVAIHELHIWAITVGK+LLACHVI+KPEANADMVLDKVIEYIRREYNISHVTIQI
Sbjct: 361 CEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 413

BLAST of Lcy02g012250 vs. NCBI nr
Match: KAG6605616.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035528.1 Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 721.8 bits (1862), Expect = 3.7e-204
Identity = 385/425 (90.59%), Postives = 398/425 (93.65%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGHSHGHGDHDHGHG HEH H+DHRHHHGISVT HHHHH HHEEEGAA D
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 289 GGK-----HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSI 348
           G K     HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQSI
Sbjct: 241 GVKHHHHHHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSI 300

Query: 349 GVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEK 408
           GVMIGGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEK
Sbjct: 301 GVMIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEK 360

Query: 409 GLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTI 468
           GLCEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTI
Sbjct: 361 GLCEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTI 420

BLAST of Lcy02g012250 vs. NCBI nr
Match: XP_022958195.1 (metal tolerance protein 1-like [Cucurbita moschata] >XP_022958198.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958199.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958200.1 metal tolerance protein 1-like [Cucurbita moschata] >XP_022958201.1 metal tolerance protein 1-like [Cucurbita moschata])

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-203
Identity = 383/423 (90.54%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQN DHGH+IEVCGDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNPDHGHMIEVCGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGHSHGHGDHDHGHG HEH H+DHRHHHGISVT HHHHH HHEEEGAA D
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 289 GGK---HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 348
           G K   HHHAHEESVT PLLE+     SQKVT TQK++RNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 GVKHHHHHHAHEESVTTPLLES----HSQKVTNTQKRERNINVQGAYLHVLGDSIQSIGV 300

Query: 349 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 408
           MIGGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 409 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 468
           CEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISH+TIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHITIQI 419

BLAST of Lcy02g012250 vs. NCBI nr
Match: XP_022995642.1 (metal tolerance protein 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 716.8 bits (1849), Expect = 1.2e-202
Identity = 381/423 (90.07%), Postives = 398/423 (94.09%), Query Frame = 0

Query: 48  EMEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKL 107
           +MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKL
Sbjct: 2   KMEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKL 61

Query: 108 FIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 167
           FIA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY
Sbjct: 62  FIAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSY 121

Query: 168 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 227
           GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL
Sbjct: 122 GFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMAL 181

Query: 228 LLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAV 287
           LLGHEHG+AHSHGHSHGHGDH+HG GGHEH H+DHRHHHGISVT HHHHH HHEEEGAA 
Sbjct: 182 LLGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAG 241

Query: 288 DGGK--HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 347
           D  K  HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQS+GV
Sbjct: 242 DSVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGV 301

Query: 348 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 407
           M+GGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 302 MVGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 361

Query: 408 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 467
           CEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 362 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 420

Query: 468 ERQ 469
           ERQ
Sbjct: 422 ERQ 420

BLAST of Lcy02g012250 vs. NCBI nr
Match: XP_022995643.1 (metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995644.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995646.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima] >XP_022995647.1 metal tolerance protein 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 716.5 bits (1848), Expect = 1.5e-202
Identity = 381/422 (90.28%), Postives = 397/422 (94.08%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVIGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGHEHG+AHSHGHSHGHGDH+HG GGHEH H+DHRHHHGISVT HHHHH HHEEEGAA D
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHNHGPGGHEHGHEDHRHHHGISVTAHHHHHPHHEEEGAAGD 240

Query: 289 GGK--HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVM 348
             K  HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQS+GVM
Sbjct: 241 SVKHQHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSVGVM 300

Query: 349 IGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLC 408
           +GGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGLC
Sbjct: 301 VGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGLC 360

Query: 409 EMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIE 468
           EM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTIQIE
Sbjct: 361 EMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQIE 418

BLAST of Lcy02g012250 vs. NCBI nr
Match: XP_023534426.1 (metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534427.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo] >XP_023534428.1 metal tolerance protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.1 bits (1847), Expect = 2.0e-202
Identity = 384/423 (90.78%), Postives = 397/423 (93.85%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           MEVQNHDHGH+IEV GDVQAVGPSIVGSKICGEAPSCGF D KNSS D +ER+ASMRKLF
Sbjct: 1   MEVQNHDHGHMIEVSGDVQAVGPSIVGSKICGEAPSCGFKDAKNSSIDMRERNASMRKLF 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IA V CIVFMS+EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG
Sbjct: 61  IAAVCCIVFMSIEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL
Sbjct: 121 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHH-HHHHEEEGAAV 288
           LGHEHG+AHSHGHSHGHGDHDHGHG HEH H+DHRHHHGISVT HHHH H HHEEEGAA 
Sbjct: 181 LGHEHGHAHSHGHSHGHGDHDHGHGSHEHGHEDHRHHHGISVTAHHHHPHPHHEEEGAAG 240

Query: 289 DGGK--HHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 348
           D  K  HHHAHEESVT PLLE+     SQKVTKTQK++RNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 DSVKHHHHHAHEESVTTPLLES----HSQKVTKTQKRERNINVQGAYLHVLGDSIQSIGV 300

Query: 349 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 408
           MIGGAIIWYKPEYKILDLICTLIFS IVL TTIQMLRNILEVLMESTPREIDATKLEKGL
Sbjct: 301 MIGGAIIWYKPEYKILDLICTLIFSVIVLYTTIQMLRNILEVLMESTPREIDATKLEKGL 360

Query: 409 CEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQI 468
           CEM+EVVA+HELHIWAITVGKVLLACHVI+KPEANADMVLD VIEYI+REYNISHVTIQI
Sbjct: 361 CEMEEVVAVHELHIWAITVGKVLLACHVIIKPEANADMVLDNVIEYIKREYNISHVTIQI 419

BLAST of Lcy02g012250 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 537.7 bits (1384), Expect = 9.1e-153
Identity = 304/419 (72.55%), Postives = 338/419 (80.67%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           ME  +  H HI+EV          IV SK+CGEAP CGFSD KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGH+HG  HSHGH HGHG            HD H H HG++VTT HHHHH HE   +   
Sbjct: 181 LGHDHG--HSHGHGHGHG------------HDHHNHSHGVTVTT-HHHHHDHEHGHSHGH 240

Query: 289 GGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVMIG 348
           G   HHAH + VT  LL    D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIG
Sbjct: 241 GEDKHHAHGD-VTEQLL----DKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIG 300

Query: 349 GAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 408
           GAIIWY PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM
Sbjct: 301 GAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEM 360

Query: 409 DEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIER 468
           +EVVA+HELHIWAITVGKVLLACHV ++PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Lcy02g012250 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 537.7 bits (1384), Expect = 9.1e-153
Identity = 304/419 (72.55%), Postives = 338/419 (80.67%), Query Frame = 0

Query: 49  MEVQNHDHGHIIEVCGDVQAVGPSIVGSKICGEAPSCGFSDVKNSSKDAKERSASMRKLF 108
           ME  +  H HI+EV          IV SK+CGEAP CGFSD KN+S DA ERSASMRKL 
Sbjct: 1   MESSSPHHSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLC 60

Query: 109 IAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYG 168
           IAVVLC+VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA+GWEATPRQ+YG
Sbjct: 61  IAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYG 120

Query: 169 FFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMALL 228
           FFRIEILGALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+L
Sbjct: 121 FFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVL 180

Query: 229 LGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVD 288
           LGH+HG  HSHGH HGHG            HD H H HG++VTT HHHHH HE   +   
Sbjct: 181 LGHDHG--HSHGHGHGHG------------HDHHNHSHGVTVTT-HHHHHDHEHGHSHGH 240

Query: 289 GGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVMIG 348
           G   HHAH + VT  LL    D    +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIG
Sbjct: 241 GEDKHHAHGD-VTEQLL----DKSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIG 300

Query: 349 GAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEM 408
           GAIIWY PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM
Sbjct: 301 GAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEM 360

Query: 409 DEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIER 468
           +EVVA+HELHIWAITVGKVLLACHV ++PEA+ADMVL+KVI+YIRREYNISHVTIQIER
Sbjct: 361 EEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNISHVTIQIER 398

BLAST of Lcy02g012250 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 522.3 bits (1344), Expect = 4.0e-148
Identity = 303/440 (68.86%), Postives = 347/440 (78.86%), Query Frame = 0

Query: 30  LFLSGFSILLISNVICLDEMEVQNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFS 89
           LFLS F   LI+      +M+   H+H H++++CG+V +   S+VG  K CGEAP CGFS
Sbjct: 37  LFLS-FDAFLIARSCFFFQMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFS 96

Query: 90  DVKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFA 149
           D K SS +A+ER+ASMRKL IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFA
Sbjct: 97  DAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFA 156

Query: 150 ISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKG 209
           ISLFSLWASGW+A P+QSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G
Sbjct: 157 ISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEG 216

Query: 210 LLMFAVSAFGLVVNIAMALLLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGI 269
            LMFAVSA GL+VNIAMA+LLGH+HG  H HGHS     HD+GHG   HSHD   H HGI
Sbjct: 217 SLMFAVSAVGLLVNIAMAILLGHDHG--HGHGHS-----HDNGHG---HSHD---HGHGI 276

Query: 270 SVTTHHHHHHHHEEEGAAVDGGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINV 329
           + T HHH   H E + + V                L+E             QKKQRN+N+
Sbjct: 277 AATEHHHDSGHDESQLSDV----------------LIE-------------QKKQRNVNI 336

Query: 330 QGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVL 389
           QGAYLHVLGDSIQS+GVMIGGAIIWYKPE+KILDLICTL+FS IVL TTI MLRNILEVL
Sbjct: 337 QGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVL 396

Query: 390 MESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKV 449
           MESTPREID T LEKG+CE++EVVA+HELHIWAITVGK+LLACHV ++PEA ADMVLDK+
Sbjct: 397 MESTPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKI 432

Query: 450 IEYIRREYNISHVTIQIERQ 469
           I+YI+RE+NISHVTIQIERQ
Sbjct: 457 IDYIKREHNISHVTIQIERQ 432

BLAST of Lcy02g012250 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.4e-147
Identity = 296/422 (70.14%), Postives = 339/422 (80.33%), Query Frame = 0

Query: 48  EMEVQNHDHGHIIEVCGDVQAVGPSIVG-SKICGEAPSCGFSDVKNSSKDAKERSASMRK 107
           +M+   H+H H++++CG+V +   S+VG  K CGEAP CGFSD K SS +A+ER+ASMRK
Sbjct: 15  KMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRK 74

Query: 108 LFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQS 167
           L IAV+LC +F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGW+A P+QS
Sbjct: 75  LLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQS 134

Query: 168 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIAMA 227
           YGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNIAMA
Sbjct: 135 YGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMA 194

Query: 228 LLLGHEHGYAHSHGHSHGHGDHDHGHGGHEHSHDDHRHHHGISVTTHHHHHHHHEEEGAA 287
           +LLGH+HG  H HGHS     HD+GHG   HSHD   H HGI+ T HHH   H E + + 
Sbjct: 195 ILLGHDHG--HGHGHS-----HDNGHG---HSHD---HGHGIAATEHHHDSGHDESQLSD 254

Query: 288 VDGGKHHHAHEESVTAPLLENCCDDDSQKVTKTQKKQRNINVQGAYLHVLGDSIQSIGVM 347
           V                L+E             QKKQRN+N+QGAYLHVLGDSIQS+GVM
Sbjct: 255 V----------------LIE-------------QKKQRNVNIQGAYLHVLGDSIQSVGVM 314

Query: 348 IGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLC 407
           IGGAIIWYKPE+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+C
Sbjct: 315 IGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVC 374

Query: 408 EMDEVVAIHELHIWAITVGKVLLACHVIVKPEANADMVLDKVIEYIRREYNISHVTIQIE 467
           E++EVVA+HELHIWAITVGK+LLACHV ++PEA ADMVLDK+I+YI+RE+NISHVTIQIE
Sbjct: 375 EIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 393

Query: 468 RQ 469
           RQ
Sbjct: 435 RQ 393

BLAST of Lcy02g012250 vs. TAIR 10
Match: AT3G61940.1 (Cation efflux family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 9.9e-115
Identity = 235/393 (59.80%), Postives = 277/393 (70.48%), Query Frame = 0

Query: 76  SKICGEAPSCGFSDVKNSSKDAKERSASMRKLFIAVVLCIVFMSVEVVGGIKANSLAILT 135
           SK+CGE  +CGFS    SS DAK+R+ASMRKL   VVLC++FMS+EVV GIKANSLAIL 
Sbjct: 6   SKVCGET-ACGFS---TSSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILA 65

Query: 136 DAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEA 195
           DAAHLL+DV AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA
Sbjct: 66  DAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEA 125

Query: 196 IVRLINGPG-EVKGLLMFAVSAFGLVVNIAMALLLGHEHGYAHSHGHSHGHGDHDHGHGG 255
           + RL+     +V G  M  V+AFGLVVNI M ++LGH+HG+ H HGHSH   DH H +G 
Sbjct: 126 VTRLVQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSH---DHGHSYG- 185

Query: 256 HEHSHDDHRHHHGISVTTHHHHHHHHEEEGAAVDGGKHHHAHEESVTAPLLENCCDDDSQ 315
                                                                   + ++
Sbjct: 186 --------------------------------------------------------ERAE 245

Query: 316 KVTKTQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVL 375
           ++ +  K+ RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FS IVL
Sbjct: 246 QLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVL 305

Query: 376 CTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKVLLACHVI 435
            TTI+MLR+ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV 
Sbjct: 306 GTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVK 334

Query: 436 VKPEANADMVLDKVIEYIRREYNISHVTIQIER 468
           V+PEA  +MVL+KVI+YI REY ISHVTIQIER
Sbjct: 366 VRPEAGDEMVLNKVIDYIWREYRISHVTIQIER 334