Lcy02g010510 (gene) Sponge gourd (P93075) v1

BLAST of Lcy02g010510 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.2e-113
Identity = 215/492 (43.70%), Postives = 306/492 (62.20%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           I ++   FLL L  +        LPPGP P P++GNLLQ+G  P       +  +G L S
Sbjct: 6   IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFS 65

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L+LGSQ  VVASSP+AAAEIL+THD  LSGRY+FQ+      +   + ++  EC+++WK 
Sbjct: 66  LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVE--NSIVWSECNDNWKL 125

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQ+ +R  KA  +V +L  K   GE V I +V F T+ N   N+
Sbjct: 126 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGK--EGEVVKIVEVVFGTLVNIFGNL 185

Query: 197 IFSKDLMEYEG--DGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFSKD+ + E    G+   KEH  K L++G   N AD++PI+  LDL G R+  AE  ++
Sbjct: 186 IFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQ 245

Query: 257 VDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTT 316
           +  V+   + ERR  +    G+ +  DF+D +L+ G  D ++  L+ E+  A T+T+++T
Sbjct: 246 IYDVWGVMLKERRGTK----GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAST 305

Query: 317 VEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPF 376
           +EW + EL K   ++ K+  E+   + D    + ES +P L YLQA VKETLRLHPP P 
Sbjct: 306 IEWAITELTKKPLVVSKIRLELVNVVGDN--TVKESDLPHLPYLQAFVKETLRLHPPTPL 365

Query: 377 LLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDF 436
           LLP RA++TC VM YTIPK+ +I VN WAIGRDP  W++P  F P+RFL SD+++KG DF
Sbjct: 366 LLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDF 425

Query: 437 RYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEK 496
             +PFG GRRICPGLP+     NLI+ATL++ F+W LP     S+L +  K G+   K+ 
Sbjct: 426 ELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDP 485

Query: 497 PLSLIPKIRVTS 507
           PL ++ K R ++
Sbjct: 486 PLLIVLKARASN 487

BLAST of Lcy02g010510 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.6e-113
Identity = 210/473 (44.40%), Postives = 297/473 (62.79%), Query Frame = 0

Query: 34  RKHSPP-LPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISLRLGSQLAVVASSPSA 93
           R  SP  LPPGP PWP++GNLLQ+G  P       +  +G L SL+LGS+  VVAS+P A
Sbjct: 16  RDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLA 75

Query: 94  AAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFLRTTCRTSLFSSKALD 153
           A+EIL+THD  LSGRY+FQ+      +   + ++  EC+E WK LR  CRT LF+ K ++
Sbjct: 76  ASEILKTHDRVLSGRYVFQSFRVKEHVE--NSIVWSECNETWKKLRKVCRTELFTQKMIE 135

Query: 154 SQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVIFSKDLMEY--EGDGA 213
           SQA +R +KA+ +V YL  K   G  V I +V F T+ N   N+IFS+++ +   E  G+
Sbjct: 136 SQAEVRESKAMEMVEYL--KKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 195

Query: 214 YGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDSVYDTFIAERRRDR 273
              KEH  + LELG   N AD++P L   DL G  K  A+C + + SV+   + ER+  +
Sbjct: 196 VEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAK 255

Query: 274 DRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEWTMAELMKNKHIMH 333
             N       DF++ +LD G  D Q+  L+ EI  A T+T+++T+EW ++EL KN  +  
Sbjct: 256 QHNNSKK--NDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTA 315

Query: 334 KVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLPHRAVQTCQVMGYT 393
            +  E+   +  +   + ES IP + YLQA VKETLRLHP  P LLP RA++TC+V+ YT
Sbjct: 316 NMRLELLSVVGKR--PVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYT 375

Query: 394 IPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLP 453
           IPK+ +I VN W IGRDP  W +P  F+P+RFL S I+FKG DF  +PFG+GRRICPG+P
Sbjct: 376 IPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVP 435

Query: 454 MGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKPLSLIPKIR 504
           +    ++LI+++L++ FDW  P   DPS+L +  K G+   KE PL ++PK R
Sbjct: 436 LATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480

BLAST of Lcy02g010510 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.3e-109
Identity = 209/490 (42.65%), Postives = 300/490 (61.22%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           + L+ +    IL LL        LPPGP PWP++GNLLQ+G  P       +  +G + +
Sbjct: 5   VALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFT 64

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L++G++  VVAS+ SAA+EIL+THD  LS RY+FQ+      +   + ++  +C E WK 
Sbjct: 65  LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKN 124

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+
Sbjct: 125 LRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK--QGEEVKIVEVIFGTLVNIFGNL 184

Query: 197 IFSKDLMEY--EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFS+++ E      G+  FKE+  + LELG   N AD++P+L   DL G RK+ AEC + 
Sbjct: 185 IFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 244

Query: 257 VDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTT 316
           + +++   + ER+  + +  G  +  DF+D  LD G  D Q+  L+ E+  A T+T+++T
Sbjct: 245 IYAIWGAMLQERKLAK-KVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 304

Query: 317 VEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPF 376
           +EW M EL KN  I  K+  EI   + ++  ++ ES  P L YL+A VKETLRLHPP P 
Sbjct: 305 IEWAMTELTKNPKITAKIRSEIQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPL 364

Query: 377 LLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDF 436
           LLP RA++TC ++ YTIPKD +I VN W IGRDP  W +P TF+P+RFL S ++F+G DF
Sbjct: 365 LLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDF 424

Query: 437 RYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEK 496
             +PFG+GRRICPGLP+    + L++AT ++  DW LPN      L +  K G+   KE 
Sbjct: 425 SLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEP 484

Query: 497 PLSLIPKIRV 505
           PL ++PK RV
Sbjct: 485 PLFIVPKSRV 487

BLAST of Lcy02g010510 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 5.0e-109
Identity = 208/490 (42.45%), Postives = 300/490 (61.22%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLIS 76
           + L+ +    IL LL        LPPGP PWP++GNLLQ+G  P       +  +G + +
Sbjct: 6   VALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFT 65

Query: 77  LRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKF 136
           L++G++  VVAS+ SAA+EIL+THD  LS RY+FQ+      +   + ++  +C E WK 
Sbjct: 66  LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKN 125

Query: 137 LRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNV 196
           LR  CRT LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+
Sbjct: 126 LRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKK--QGEEVKIVEVIFGTLVNIFGNL 185

Query: 197 IFSKDLME--YEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 256
           IFS+++ E      G+  FKE+  + LELG   N AD++P+L   DL G RK+ AEC + 
Sbjct: 186 IFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 245

Query: 257 VDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTT 316
           + +++   + ER+  + +  G  +  DF+D  LD G  D Q+  L+ E+  A T+T+++T
Sbjct: 246 IYAIWGAMLQERKLAK-KVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 305

Query: 317 VEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPF 376
           +EW M EL KN  I  K+  E+   + ++  ++ ES  P L YL+A VKETLRLHPP P 
Sbjct: 306 IEWAMTELTKNPKITAKLRSELQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPL 365

Query: 377 LLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDF 436
           LLP RA++TC ++ YTIPKD +I VN W IGRDP  W +P TF+P+RFL S ++F+G DF
Sbjct: 366 LLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDF 425

Query: 437 RYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEK 496
             +PFG+GRRICPGLP+    + L++AT ++  DW LPN      L +  K G+   KE 
Sbjct: 426 SLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEP 485

Query: 497 PLSLIPKIRV 505
           PL ++PK RV
Sbjct: 486 PLFIVPKSRV 488

BLAST of Lcy02g010510 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 8.6e-101
Identity = 203/491 (41.34%), Postives = 302/491 (61.51%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPN---PL-LAITHFSTLHGP 77
           L  L+P +L+  LL   K+   LPPGP PWPL+GNL  +  N   PL + + + +  HGP
Sbjct: 7   LFSLIPVILVFILLLKPKYK-NLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGP 66

Query: 78  LISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEH 137
           ++ L LG+Q  V+AS+  AA EIL+THD   S R+I  +    H + ++SL+ S +C ++
Sbjct: 67  IMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHI-KYSLVWS-DCTDY 126

Query: 138 WKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTM 197
           WK LR   RT +FS K L +Q+ +R  K   L+ +L SK   G+ V I    F T+ N +
Sbjct: 127 WKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSK--EGQVVKISQFVFGTLLNIL 186

Query: 198 TNVIFSKDLMEY-EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECS 257
            NV+FSKD+  Y +     G +   R+ L +G  PN+A+FYP L  LDLQGL+KK  E  
Sbjct: 187 GNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERF 246

Query: 258 RRVDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTS 317
            RV  +++  + ER+ +R+        +D LD +L   F D Q+  L  E     ++T+S
Sbjct: 247 IRVMKMWEGTVKERKANRNEES-----KDMLDVLLANDFNDAQINALFLETFGPGSETSS 306

Query: 318 TTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPA 377
            T+EW +AEL+K+   M KV +E+   +    + I ES +PQL YLQAC+KE +RLHP A
Sbjct: 307 ATIEWVIAELIKSPKEMAKVRKELNEVV--GTSTIKESDLPQLPYLQACIKEAMRLHPAA 366

Query: 378 PFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGF 437
           PFLLP RA +TC+VMGYTIPK++++ VN +AIGRDP +W++PSTF P+RFL SD++F G 
Sbjct: 367 PFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGA 426

Query: 438 DFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYK 497
            ++++PFGSGRR C G+P+    + LI+ +L+  +D+ LP    P  L ++  + +    
Sbjct: 427 HYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAI 485

Query: 498 EKPLSLIPKIR 504
           +  L ++PK R
Sbjct: 487 DPSLCVVPKAR 485

BLAST of Lcy02g010510 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 6.9e-154
Identity = 276/488 (56.56%), Postives = 354/488 (72.54%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL     IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 315
           DS ++  I ERR    R+  AA+  DFLD +++ GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSRDVSAAS--DFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 316 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 375
           EW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 376 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 435
           LP  A + C++MGY++PKDT IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYR 427

Query: 436 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 495
           +LPFG GRRICPGLPM  VQV LILATLI  F+WYLPN+ED S LDL+G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 496 LSLIPKIR 504
           L L+ K R
Sbjct: 488 LKLLSKKR 489

BLAST of Lcy02g010510 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 8.5e-152
Identity = 272/488 (55.74%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL    +IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++GPLI
Sbjct: 8   FLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 315
           DS ++  I ERR    +  G +   DFLD ++  GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSQ--GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 316 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 375
           EW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 376 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 435
           LP  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 427

Query: 436 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 495
           +LPFG GRRICPGLPM  VQV LILATLI  F+W LPN+ED S LDL+G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 496 LSLIPKIR 504
           L L+ K R
Sbjct: 488 LKLLSKNR 489

BLAST of Lcy02g010510 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 6.1e-150
Identity = 269/493 (54.56%), Postives = 349/493 (70.79%), Query Frame = 0

Query: 13  MAPFILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           +A F  LLLLP +  ILK + S    PP PPGP PWP+LGNLLQIG N  +++T FS ++
Sbjct: 3   IASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIY 62

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ + D       SL+ SPEC 
Sbjct: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECG 122

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           + WK LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFN
Sbjct: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKEFVFITVFN 182

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N IFS+DL+ + GDG  G + H++  ++  L+PNLADFYP+L   DLQGLRKK+A  
Sbjct: 183 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 242

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTT 312
             ++DS ++  I ERR    R  G     DFLD ++  GF D+Q+ YL+ E+LSA TDTT
Sbjct: 243 KNQIDSSWEILIKERRTIHSR--GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 302

Query: 313 STTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPP 372
           +TTVEW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPP
Sbjct: 303 TTTVEWAMAELLKNRDIMNRVREETKKAINGD--AIDESKLSELHYLWKCVKETLRLHPP 362

Query: 373 APFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKG 432
           APFLLP  A + C++MGY++PK T I VN+W IGRDP+ WE+P +F P+RF   D + KG
Sbjct: 363 APFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKG 422

Query: 433 FDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCY 492
            D+R+LPFG GRR+CPGLPM  VQV LILATLI  F+W LPN+ED S LDL G++G+   
Sbjct: 423 HDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQ 482

Query: 493 KEKPLSLIPKIRV 505
           KE+PL L+PK R+
Sbjct: 483 KEEPLKLLPKRRI 489

BLAST of Lcy02g010510 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 1.0e-149
Identity = 272/491 (55.40%), Postives = 354/491 (72.10%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISL 77
           LLLLLP  +ILK +KS     PLPPGP PWP+LGNLLQIG N  ++IT F+ ++GPLISL
Sbjct: 13  LLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISL 72

Query: 78  RLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFL 137
           +LG+Q  +VASSP+AA  +L+T D  LS RYIFQ   D     + SL+ SPEC + WK L
Sbjct: 73  KLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWKNL 132

Query: 138 RTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVI 197
           R+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT++N+I
Sbjct: 133 RSLCKVNLFTAKAIESQAILREKKMKELVKFLESK--QGSVVEVKDFVFTSVFNTLSNLI 192

Query: 198 FSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDS 257
           FS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGL+ K+      VDS
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 258 VYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEW 317
           ++   I ERR    + G A+   DFLD ++  GF D Q+  L+ E+LSA TDTT+TTVEW
Sbjct: 253 LWGILIKERREIHCQQGSAS--NDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 312

Query: 318 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 377
            M+E++K+K I++KV EE+ +++  +N  IDES I +L YL  CVKETLRLHPPAPFLLP
Sbjct: 313 AMSEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLP 372

Query: 378 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSD-IEFKGFD 437
             A   C+VMGY+IPKD+ +FVN+W IGRDP+ WE+  TFNP RF    G+D ++FKG+D
Sbjct: 373 RLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYD 432

Query: 438 FRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKE 497
           +RYLPFG GRRICPGLPM IVQV LILATLI  F+W LPN+ED SKLDL+G++G+   K 
Sbjct: 433 YRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKY 492

Query: 498 KPLSLIPKIRV 505
           + L LIPK RV
Sbjct: 493 ETLKLIPKRRV 498

BLAST of Lcy02g010510 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 1.4e-149
Identity = 272/491 (55.40%), Postives = 354/491 (72.10%), Query Frame = 0

Query: 18  LLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLISL 77
           LLLLLP  +ILK +KS     PLPPGP PWP+LGNLLQIG N  ++IT F+ ++GPLISL
Sbjct: 13  LLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISL 72

Query: 78  RLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWKFL 137
           +LG+Q  +VASSP+AA  +L+T D  LS RYIFQ   D     + SL+ SPEC + WK L
Sbjct: 73  KLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWKNL 132

Query: 138 RTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTNVI 197
           R+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT++N+I
Sbjct: 133 RSLCKVNLFTAKAIESQAILREKKMKELVKFLESK--QGSVVEVKDFVFTSVFNTLSNLI 192

Query: 198 FSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRVDS 257
           FS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGL+ K+      VDS
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 258 VYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEW 317
           ++   I ERR    + G A+   DFLD ++  GF D Q+  L+ E+LSA TDTT+TTVEW
Sbjct: 253 LWGILIKERREIHCQQGSAS--NDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 312

Query: 318 TMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFLLP 377
            M+E++K+K I++KV EE+ +++  +N  IDES I +L YL  CVKETLRLHPPAPFLLP
Sbjct: 313 AMSEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLP 372

Query: 378 HRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSD-IEFKGFD 437
             A   C+VMGY+IPKD+ +FVN+W IGRDP+ WE+  TFNP RF    G+D ++FKG+D
Sbjct: 373 RLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYD 432

Query: 438 FRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKE 497
           +RYLPFG GRRICPGLPM IVQV LILATLI  F+W LPN+ED SKLDL+G++G+   K 
Sbjct: 433 YRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKY 492

Query: 498 KPLSLIPKIRV 505
           + L LIPK RV
Sbjct: 493 ETLKLIPKRRV 498

BLAST of Lcy02g010510 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 553.9 bits (1426), Expect = 1.4e-153
Identity = 276/488 (56.56%), Postives = 354/488 (72.54%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL     IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 315
           DS ++  I ERR    R+  AA+  DFLD +++ GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSRDVSAAS--DFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 316 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 375
           EW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 376 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 435
           LP  A + C++MGY++PKDT IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYR 427

Query: 436 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 495
           +LPFG GRRICPGLPM  VQV LILATLI  F+WYLPN+ED S LDL+G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 496 LSLIPKIR 504
           L L+ K R
Sbjct: 488 LKLLSKKR 489

BLAST of Lcy02g010510 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 547.0 bits (1408), Expect = 1.7e-151
Identity = 272/488 (55.74%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+LLLL    +IL+ + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++GPLI
Sbjct: 8   FLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHWK 135
           SL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 136 FLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMTN 195
            LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKELVFITVFNTLSN 187

Query: 196 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRRV 255
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQV 247

Query: 256 DSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 315
           DS ++  I ERR    +  G +   DFLD ++  GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSQ--GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 316 EWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPFL 375
           EW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 376 LPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDFR 435
           LP  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYR 427

Query: 436 YLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEKP 495
           +LPFG GRRICPGLPM  VQV LILATLI  F+W LPN+ED S LDL+G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 496 LSLIPKIR 504
           L L+ K R
Sbjct: 488 LKLLSKNR 489

BLAST of Lcy02g010510 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.4 bits (1404), Expect = 5.1e-151
Identity = 273/489 (55.83%), Postives = 354/489 (72.39%), Query Frame = 0

Query: 16  FILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPL 75
           F+LLLLLP ++ ILK + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++GPL
Sbjct: 8   FLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 67

Query: 76  ISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEHW 135
           ISL+LG+Q  VVASSP+AAA IL+T D  LS RYIFQ + D       SL+ SPEC + W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 127

Query: 136 KFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTMT 195
           K LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFNT++
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSK--QGSVVEIKEFVFITVFNTLS 187

Query: 196 NVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSRR 255
           N IFS+DL+ + GDG  G K H++  ++L L+PNLADFYP+L   DLQGLRKK+A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 247

Query: 256 VDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTT 315
           +DS ++  I ERR    R   AAT  DFLD ++  G+ ++Q+ YL+ E+LSA TDT  TT
Sbjct: 248 IDSSWEILIKERRTIHSRGVSAAT--DFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TT 307

Query: 316 VEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPPAPF 375
           VEW MAEL+KN+ IM++V EE  + I     AIDES + +LHYL  CVKETLRLHPPAPF
Sbjct: 308 VEWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPF 367

Query: 376 LLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKGFDF 435
           LLP  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF G D + KG D+
Sbjct: 368 LLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDY 427

Query: 436 RYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCYKEK 495
           R+LPFG GRR+CPGLPM  VQV LILATLI  F+W LPN+E+ S LDL+G++GI   KE+
Sbjct: 428 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEE 487

Query: 496 PLSLIPKIR 504
           PL L+PK R
Sbjct: 488 PLKLLPKKR 488

BLAST of Lcy02g010510 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.4 bits (1404), Expect = 5.1e-151
Identity = 271/492 (55.08%), Postives = 352/492 (71.54%), Query Frame = 0

Query: 13  MAPFILLLLLPFLL-ILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           +A F  LLLLP +  +LK + S    PPLPPGP PWP+LGNLLQIG N  +++T FS ++
Sbjct: 3   IASFSFLLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIY 62

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+AAA IL+T D  LS RYI Q   D       SLL SPEC 
Sbjct: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECG 122

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           + WK LR+ CR  LF+++A++SQA+LRR K   +V +L SK   G  V I++  F TVFN
Sbjct: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSK--QGSVVEIKEFVFITVFN 182

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N+IFS+DL+++ GDG  G K  ++  ++LGL+PNLADFYP+L   DLQGLRKK+A  
Sbjct: 183 TLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIY 242

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTT 312
             ++DS  +  I ERR    R  G +   DFLD +++ GF ++Q+ YL+ E+L A TDTT
Sbjct: 243 KNQIDSSSEILIKERRTIHSR--GVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTT 302

Query: 313 STTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPP 372
           +TTVEW MAELMKN+ IM++V EE  + +     AIDES + +LHYL  CVKET+RLHPP
Sbjct: 303 TTTVEWAMAELMKNRDIMNRVREETKKAL--NGGAIDESKLSELHYLWKCVKETVRLHPP 362

Query: 373 APFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGSDIEFKG 432
           APFLLP  A + C++MGY++PK T IFVN+W IGRDP+ WE+P +F P+RF  SD + KG
Sbjct: 363 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKG 422

Query: 433 FDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGIVCY 492
            D+R+LPFG GRR CPGLPM  VQV LILATLI  F+W LPN+ED S LDL+G++GI   
Sbjct: 423 HDYRFLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQ 482

Query: 493 KEKPLSLIPKIR 504
           KE+PL L+PK R
Sbjct: 483 KEEPLKLVPKKR 488

BLAST of Lcy02g010510 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 544.7 bits (1402), Expect = 8.7e-151
Identity = 273/493 (55.38%), Postives = 357/493 (72.41%), Query Frame = 0

Query: 13  MAPFILLLLLPFLLILKLLKSRK-HSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLH 72
           + P +LLL+ P  +ILK +KS      PLPPGP PWP+LGNL QIG N  ++IT F+ ++
Sbjct: 9   LLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIY 68

Query: 73  GPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECD 132
           GPLISL+LG+Q  VVASSP+ A  +L+THD  LS RYI Q   D     + SL+ SPEC 
Sbjct: 69  GPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECG 128

Query: 133 EHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFN 192
           +HWK LR+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V I+D  FT+VFN
Sbjct: 129 DHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESK--QGSVVKIKDFVFTSVFN 188

Query: 193 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAEC 252
           T++N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   DLQGLRKK+A  
Sbjct: 189 TLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIY 248

Query: 253 SRRVDSVYDTFIAERRRDRDRNGGAATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTT 312
              VDS++   I ERR    + G A++  DFLD ++  GF D Q+ YL+ E+LSA TDTT
Sbjct: 249 MNEVDSLWGILIKERREIHCQQGSASS--DFLDVLIQSGFSDQQINYLINELLSAGTDTT 308

Query: 313 STTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKETLRLHPP 372
           +TTVEW M+E++K+K I++KV +EI +  K+  + IDES I +L YL +CVKETLRLHPP
Sbjct: 309 TTTVEWAMSEILKDKGILNKVRQEIKKVTKE--SPIDESNISKLQYLWSCVKETLRLHPP 368

Query: 373 APFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF---LGSDIE 432
           APFLLP  A   C+VMGY+IPKD+ IFVN+W IGRDP+ WE+  TFNP+RF    G++++
Sbjct: 369 APFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVD 428

Query: 433 FKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGKMGI 492
           FKG+D+RYLPFG GRRICPGLPM IVQV LILATL+  F+W LPN ED SKLDL+G++G+
Sbjct: 429 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGV 488

Query: 493 VCYKEKPLSLIPK 502
              K + L LIPK
Sbjct: 489 TLQKYEALELIPK 495

BLAST of Lcy02g010510 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 360.5 bits (924), Expect = 2.2e-99
Identity = 201/499 (40.28%), Postives = 283/499 (56.71%), Query Frame = 0

Query: 16  FILLLLLPFLLILKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGPLI 75
           F+L   + F    +   SRK   P PPGP   P++GN+  +G NP  +    S  +GP++
Sbjct: 15  FVLSFFIIFFTTTRPRSSRK-VVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIM 74

Query: 76  SLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLR-----RFSLLLSPEC 135
           SL+ GS   VV +SP AA E+LRT+D  LS R      T T+ +R     + S++  P  
Sbjct: 75  SLKFGSLNTVVVTSPEAAREVLRTYDQILSSR------TPTNSIRSINHDKVSVVWLPPS 134

Query: 136 DEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVF 195
              W+ LR    T LFS + +++   LR NK   LV +++  +E  E V I    F T  
Sbjct: 135 SSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITAL 194

Query: 196 NTMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAE 255
           N ++N++FS DL  Y+ + +  F++     +E    P+ A+F+P L  LDLQG RK    
Sbjct: 195 NIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKA 254

Query: 256 CSRRVDSVYDTFIAERRRD---RDRNGGAATCRDFLDSMLDFGFCDD------QMRYLMA 315
           CS R+  V+  FI  +  +   RD N      RDF+D +LD    D+       + +L+ 
Sbjct: 255 CSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLL 314

Query: 316 EILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQAC 375
           ++  A TDT S+TVEW MAEL++N   M K   EI   I  Q   ++ES I  L YLQA 
Sbjct: 315 DLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVI-GQKGVVEESDISALPYLQAV 374

Query: 376 VKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDR 435
           VKET RLHP AP L+P +A    +V+G+ +PKDT++FVN+WAIGRDP  WEN S F P+R
Sbjct: 375 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPER 434

Query: 436 FLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLD 495
           FLG DI+ +G D+   PFG+GRRICPGLP+ +  V L+LA+L+  FDW LPN      LD
Sbjct: 435 FLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLD 494

Query: 496 LSGKMGIVCYKEKPLSLIP 501
           +    G+  +K  PL  +P
Sbjct: 495 MDETFGLTLHKTNPLHAVP 505

BLAST of Lcy02g010510 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 349.7 bits (896), Expect = 3.8e-96
Identity = 193/503 (38.37%), Postives = 289/503 (57.46%), Query Frame = 0

Query: 10  VKAMAPFILLLLL-PFLLILKLLKSRKHS---PPLPPGPTPWPLLGNLLQIGPNPLLAIT 69
           +   A F+L   +    LI    +SR+ S     LPPGP   P++GN+ Q+G NP  +  
Sbjct: 4   ISGQALFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFA 63

Query: 70  HFSTLHGPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLL 129
             + ++GP++SL+ G   +VV +SP AA E+LRTHD  LSGR    ++         S++
Sbjct: 64  DLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIR-CFGHEEVSVI 123

Query: 130 LSPECDEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVA 189
             P     W+ LR    T +FS +  ++  ALR  K   LV ++   +E  E V I   +
Sbjct: 124 WLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRAS 183

Query: 190 FTTVFNTMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLR 249
           +TTV N ++N++FS DL  Y+   +  F++     +E    P+ A+++P +  LDLQG R
Sbjct: 184 YTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNR 243

Query: 250 KKSAECSRRVDSVYDTFIAERRRDRDRNGGA--ATCRDFLDSMLDFGFCDD------QMR 309
           K     + R+  V+  F+  +  ++     +   + RDFLDS+L     D+       + 
Sbjct: 244 KAMRGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIE 303

Query: 310 YLMAEILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHY 369
           +L+ ++ +A TDT+S+T+EW MAEL++N   M K   E+ R +  QN+ + ES I  L Y
Sbjct: 304 HLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDR-VLGQNSVVQESDISGLPY 363

Query: 370 LQACVKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTF 429
           LQA VKET RLHP AP L+P +A    +V+G+ +PKDT++ VN+WAIGRDP+ WENPS F
Sbjct: 364 LQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQF 423

Query: 430 NPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDP 489
            P+RF+G DI+ KG D+   PFG GRRICPGLP+ +  V+L+LA+L+  FDW LPN    
Sbjct: 424 EPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVS 483

Query: 490 SKLDLSGKMGIVCYKEKPLSLIP 501
             LD+    GI  ++   L  IP
Sbjct: 484 EDLDMDETFGITLHRTNTLYAIP 504

BLAST of Lcy02g010510 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 348.6 bits (893), Expect = 8.5e-96
Identity = 197/495 (39.80%), Postives = 284/495 (57.37%), Query Frame = 0

Query: 17  ILLLLLPFLLILKLLKSRKHSPPL---PPGPTPWPLLGNLLQIGPNPLLAITHFSTLHGP 76
           I   +L  LL     +SR+    L   PPGP   P++GN+  +G NP  + T  S  +GP
Sbjct: 12  IFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGP 71

Query: 77  LISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPECDEH 136
           ++SL+LG   +VV +S  A  E+L+THD  LSGRYI +  T ++    FS+         
Sbjct: 72  VMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISE-ATKSNNHHEFSVGWIHPSSSR 131

Query: 137 WKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVFNTM 196
           ++ LR    T LFS + + +  ALR  K   LV++L+   E  E V I  V+F T  N +
Sbjct: 132 FRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNII 191

Query: 197 TNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSAECSR 256
           +N++FS +L  Y+   +  F+E      E    P+LA+F+P +  LDLQG  KK  E S 
Sbjct: 192 SNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSG 251

Query: 257 RVDSVYDTFIAERRRDRDRNG--GAATCRDFLDSMLDFGFCD------DQMRYLMAEILS 316
           R+  V+  F   R  ++         + +DFLD ++D    D      D++ +L+ ++  
Sbjct: 252 RLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHLLLDMFV 311

Query: 317 ASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACVKET 376
           A TDT S+TVEW MAEL+ N   M KV +EI   I  QN    ES I +L YL+A VKET
Sbjct: 312 AGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVI-GQNGDFQESDISKLPYLKAVVKET 371

Query: 377 LRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRFLGS 436
            RLHP APFLL  +A    +++G+T+ KD+++ VN+WAIGRDP  WENP+ F P+RFLG 
Sbjct: 372 FRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERFLGK 431

Query: 437 DIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDLSGK 496
           +I+ KG D+   PFG+GRRICPGLP+ +  V+L+LA+L+  F+W LPN      LD+   
Sbjct: 432 EIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEET 491

Query: 497 MGIVCYKEKPLSLIP 501
            G+  +K  PL  +P
Sbjct: 492 FGLTVHKTNPLLAVP 504

BLAST of Lcy02g010510 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 346.7 bits (888), Expect = 3.2e-95
Identity = 197/514 (38.33%), Postives = 292/514 (56.81%), Query Frame = 0

Query: 7   VSRVKAMAPFILLLLLPFLLILKLLKSRKHSP--PLPPGPTPWPLLGNLLQIGPNPLLAI 66
           +S ++ M+  +  LL  F       K+R+ S    LPPGP   PL+GN+ Q+G NP  ++
Sbjct: 3   LSLIQGMSLPLYFLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSL 62

Query: 67  THFSTLHGPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSL 126
             FS  +GP++SL+LG   AVV SSP AA E LRTHD  +S R  F          + S+
Sbjct: 63  AAFSKTYGPIMSLKLGRLTAVVISSPEAAKEALRTHDHVMSAR-TFNDALRAFDHHKHSI 122

Query: 127 LLSPECDEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDV 186
           +  P     W+FL+ T    L S + LD+  +LR  K   LV  +    E GE + +   
Sbjct: 123 VWIPP-SARWRFLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARA 182

Query: 187 AFTTVFNTMTNVIFSKDLMEYEGD-GAYGFKEHYRKTLELGLVPNLADFYPILSGLDLQG 246
           +F T FN ++N +FS DL  Y+ +  +Y F        ++  +PN+ D++  +  LDLQG
Sbjct: 183 SFVTSFNIISNALFSVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQG 242

Query: 247 LRKKSAECSRRVDSVYDTFI----AERRRDRDRNGGAATCRDFLDSMLDF------GFCD 306
            RKK+  C  ++  V+  FI    A+R    ++    A+  D LDS+LD           
Sbjct: 243 TRKKAVLCIEKLFRVFQEFIDARLAKRFSRTEKEPKEASSIDMLDSLLDLTQQNEAELTM 302

Query: 307 DQMRYLMAEILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIP 366
           + +++L+ ++  A TDT S+T+EW M EL ++   M K   EI R++  QN  + ES IP
Sbjct: 303 NDLKHLLLDVFVAGTDTNSSTMEWAMTELFRSTEKMVKAQSEI-RQVIGQNGFVQESDIP 362

Query: 367 QLHYLQACVKETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWEN 426
            L YLQA VKETLRLHP AP L+P ++    Q+MG+ +PK+T++ VN+WAIGRD + WEN
Sbjct: 363 SLPYLQAIVKETLRLHPAAP-LIPRKSESDVQIMGFLVPKNTQVVVNVWAIGRDASVWEN 422

Query: 427 PSTFNPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPN 486
           P  F P+RFL  + + KG DF  +PFGSGRR+CPG+ M +  ++++LA+L+  FDW L N
Sbjct: 423 PMKFEPERFLLRETDVKGRDFELIPFGSGRRMCPGISMALKTMHMVLASLLYSFDWKLQN 482

Query: 487 NEDPSKLDLSGKMGIVCYKEKPLSLIPKIRVTSS 508
              P  +D+S   G+  +K K L  +P  + T S
Sbjct: 483 GVVPGNIDMSETFGLTLHKAKSLCAVPVKKPTIS 512

BLAST of Lcy02g010510 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 342.4 bits (877), Expect = 6.1e-94
Identity = 188/498 (37.75%), Postives = 285/498 (57.23%), Query Frame = 0

Query: 18  LLLLLPFLLILKLL-------KSRKHSPPLPPGPTPWPLLGNLLQIGPNPLLAITHFSTL 77
           LLLL  F+L   L+       +  + +  LPPGP   P++GN+  +G +P  +    S  
Sbjct: 9   LLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKT 68

Query: 78  HGPLISLRLGSQLAVVASSPSAAAEILRTHDCTLSGRYIFQTVTDTHKLRRFSLLLSPEC 137
           +GP++SL+LGS   VV +SP AA E+LRTHD  LS R     V   +  +  SL+  P  
Sbjct: 69  YGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSINH-QDASLVWLPSS 128

Query: 138 DEHWKFLRTTCRTSLFSSKALDSQAALRRNKALALVHYLASKTETGEPVAIRDVAFTTVF 197
              W+ LR    T L S + +++  ALR NK   LV +++  ++  E V I  VAF T  
Sbjct: 129 SARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTL 188

Query: 198 NTMTNVIFSKDLMEYEGDGAY-GFKEHYRKTLELGLVPNLADFYPILSGLDLQGLRKKSA 257
           N ++N++FS DL  Y    +  G ++     ++    P+ A+++P L  LDLQG  K   
Sbjct: 189 NIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFK 248

Query: 258 ECSRRVDSVYDTFIAER--RRDRDRNGGAATCRDFLDSMLDFGFCDDQM-----RYLMAE 317
            C+ R+  V+  FI  +   +    N    +  DF+D++LD+   + ++      +L+ +
Sbjct: 249 VCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHLLLD 308

Query: 318 ILSASTDTTSTTVEWTMAELMKNKHIMHKVHEEITREIKDQNAAIDESMIPQLHYLQACV 377
           + +A TDT+S+T+EW M EL+KN   M K   EI   I  QN  ++ES I +L YLQA V
Sbjct: 309 MFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVI-GQNGIVEESDISKLPYLQAVV 368

Query: 378 KETLRLHPPAPFLLPHRAVQTCQVMGYTIPKDTRIFVNIWAIGRDPTAWENPSTFNPDRF 437
           KET RLH P P L+P +A    +++G+ + KDT++ VN+WAIGRDP+ W+NPS F P+RF
Sbjct: 369 KETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF 428

Query: 438 LGSDIEFKGFDFRYLPFGSGRRICPGLPMGIVQVNLILATLIRKFDWYLPNNEDPSKLDL 497
           LG D++ +G D+   PFG+GRRICPG+P+ +  V+L+LA+L+  FDW LP       LD+
Sbjct: 429 LGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDM 488

Query: 498 SGKMGIVCYKEKPLSLIP 501
               G+  +K  PL  +P
Sbjct: 489 DETFGLTLHKTNPLHAVP 504