Lcy01g006690 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g006690
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr01: 10488210 .. 10488740 (-)
RNA-Seq ExpressionLcy01g006690
SyntenyLcy01g006690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCATGGACCCCTGATCAACTGCGTGAAAATCAACGTTGATGTTTCATGGAACAAGGAGCTGAAAAGAGGAGTGGGTTGGATTATCTGTGATTCTCTCGGATCTCCGATGGGAGTTGGCTACGCTAAAATCCAGAGAAATTGGTCGATCAAAGCCCTTGAGACGTTAGCGATTAGGAAAGGGTTAGATGCGTACCTTTCCTCCAAGCGTGAGCCGTCCTTCCGAGTCATTGTCGAATCAGACTCGACGGACGTGGTGAGGGCTCTGAACCATGAATGGGTTGATGCATCGAAGTCGAAGCTGGTGATGGATGATATCGAAGAGCTAGCTGCTCTGGTTGGTGTGATTGCGTTCGTAAAATGCCCTCGAGCAAGCAACAAAGTCGCACATGTCCTCACGCGAGCTACTGTTGGATTTCGATCCAAGGAAGTCCCGTCTTCCATTGTTGTAGACGGTTTTTTTGTGCTCGTTCCATCTTCCACGTTGGAAGATAATTCGTTTGGTTGGGAAGGGAAAATCCCTTTTTGA

mRNA sequence

ATGAGTCATGGACCCCTGATCAACTGCGTGAAAATCAACGTTGATGTTTCATGGAACAAGGAGCTGAAAAGAGGAGTGGGTTGGATTATCTGTGATTCTCTCGGATCTCCGATGGGAGTTGGCTACGCTAAAATCCAGAGAAATTGGTCGATCAAAGCCCTTGAGACGTTAGCGATTAGGAAAGGGTTAGATGCGTACCTTTCCTCCAAGCGTGAGCCGTCCTTCCGAGTCATTGTCGAATCAGACTCGACGGACGTGGTGAGGGCTCTGAACCATGAATGGGTTGATGCATCGAAGTCGAAGCTGGTGATGGATGATATCGAAGAGCTAGCTGCTCTGGTTGGTGTGATTGCGTTCGTAAAATGCCCTCGAGCAAGCAACAAAGTCGCACATGTCCTCACGCGAGCTACTGTTGGATTTCGATCCAAGGAAGTCCCGTCTTCCATTGTTGTAGACGGTTTTTTTGTGCTCGTTCCATCTTCCACGTTGGAAGATAATTCGTTTGGTTGGGAAGGGAAAATCCCTTTTTGA

Coding sequence (CDS)

ATGAGTCATGGACCCCTGATCAACTGCGTGAAAATCAACGTTGATGTTTCATGGAACAAGGAGCTGAAAAGAGGAGTGGGTTGGATTATCTGTGATTCTCTCGGATCTCCGATGGGAGTTGGCTACGCTAAAATCCAGAGAAATTGGTCGATCAAAGCCCTTGAGACGTTAGCGATTAGGAAAGGGTTAGATGCGTACCTTTCCTCCAAGCGTGAGCCGTCCTTCCGAGTCATTGTCGAATCAGACTCGACGGACGTGGTGAGGGCTCTGAACCATGAATGGGTTGATGCATCGAAGTCGAAGCTGGTGATGGATGATATCGAAGAGCTAGCTGCTCTGGTTGGTGTGATTGCGTTCGTAAAATGCCCTCGAGCAAGCAACAAAGTCGCACATGTCCTCACGCGAGCTACTGTTGGATTTCGATCCAAGGAAGTCCCGTCTTCCATTGTTGTAGACGGTTTTTTTGTGCTCGTTCCATCTTCCACGTTGGAAGATAATTCGTTTGGTTGGGAAGGGAAAATCCCTTTTTGA

Protein sequence

MSHGPLINCVKINVDVSWNKELKRGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGLDAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCPRASNKVAHVLTRATVGFRSKEVPSSIVVDGFFVLVPSSTLEDNSFGWEGKIPF
Homology
BLAST of Lcy01g006690 vs. ExPASy TrEMBL
Match: A0A6P5S2T0 (uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-08
Identity = 49/140 (35.00%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 5   PLINCVKINVDVSWNKELKR-GVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGL 64
           P  + +K+N DV+W  +L R GVGW+I DS G  +  G     R  S   +E LAIR  L
Sbjct: 79  PPPSLLKVNCDVAWFAQLLRGGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHAL 138

Query: 65  DAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCP 124
            A    +      V+VESD+   +  LN +    S  + ++ DI++L A    ++FV  P
Sbjct: 139 SACAHFQLT---HVMVESDTQRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAP 198

Query: 125 RASNKVAHVLTRATVGFRSK 144
           ++ N+VAH +     GF SK
Sbjct: 199 KSCNRVAHTV----AGFVSK 211

BLAST of Lcy01g006690 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-07
Identity = 42/126 (33.33%), Postives = 64/126 (50.79%), Query Frame = 0

Query: 10   VKINVDVSWNKELKR-GVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGLDAYLS 69
            +K+N D +W  + +  GVGW++ DS G  +  G     R  S  A+E  AIR+ L A + 
Sbjct: 1067 LKVNCDAAWVSQTQMGGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQ 1126

Query: 70   SKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCPRASNK 129
            S      ++ VESDS  ++R L  EW      + ++ DI++L        F+  PR  NK
Sbjct: 1127 SGLS---KLEVESDSLQLIRMLGGEWNIDIAVETIVFDIKQLVCQFQHCVFLYTPRNCNK 1186

Query: 130  VAHVLT 135
             AH +T
Sbjct: 1187 AAHKIT 1189

BLAST of Lcy01g006690 vs. ExPASy TrEMBL
Match: M7ZXZ0 (Laccase OS=Triticum urartu OX=4572 GN=TRIUR3_26891 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.4e-07
Identity = 43/134 (32.09%), Postives = 73/134 (54.48%), Query Frame = 0

Query: 5   PLINCVKINVDVSWN-KELKRGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGL 64
           P  + VK+NVD S++  +L+  VG ++ D  G  +     +++    ++  E  A+R G+
Sbjct: 580 PQEDFVKLNVDASFHADDLQGAVGAVLRDCRGGFIASSNERLEHVVDVETAEAHALRHGI 639

Query: 65  DAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGV--IAFVK 124
              L ++R    ++IVESD  +V+  +N+    AS S  +  D   L  ++G   ++FV 
Sbjct: 640 ---LLAQRMGITKLIVESDCLEVINTMNNGGFTASGSAAIYSDC--LVFIIGYTSVSFVH 699

Query: 125 CPRASNKVAHVLTR 136
           CPR +N VAHVL R
Sbjct: 700 CPRKTNYVAHVLVR 708

BLAST of Lcy01g006690 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.8e-07
Identity = 42/128 (32.81%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 5    PLINCVKINVDVSWNKELKR-GVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGL 64
            P    +K+N D +W  + +  GVGW++ DS G  +  G     R  S  A+E  AIR+ L
Sbjct: 1150 PPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIREAL 1209

Query: 65   DAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCP 124
             A + S      ++ VESDS  V+R +  EW        ++ DI++L        F+  P
Sbjct: 1210 VACVQSGLS---KLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCVFLYTP 1269

Query: 125  RASNKVAH 132
            R  NK AH
Sbjct: 1270 RNCNKAAH 1274

BLAST of Lcy01g006690 vs. ExPASy TrEMBL
Match: A0A803L9N8 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.2e-06
Identity = 41/151 (27.15%), Postives = 72/151 (47.68%), Query Frame = 0

Query: 5   PLINCVKINVDVSWNKELKRGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGLD 64
           P  +  K+N D S +K+ + G+G ++ D++G  M      +     I  +E +A+  G+ 
Sbjct: 130 PTSSRYKLNTDASISKDRRGGLGMVVRDNIGDVMMTWGKSLNGTGIISEIEAVALLFGMR 189

Query: 65  AYLSSKREPSFRVI-VESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCP 124
           A      +  FR + V SD   V+  LN    + S ++L++ DI   A     + F  CP
Sbjct: 190 AVF----DAGFRKLEVSSDCLQVIELLNGSKHENSPTQLIVTDILSFAYSFDFVCFSFCP 249

Query: 125 RASNKVAHVLTRATVG-----FRSKEVPSSI 150
           R  NKVAH +   ++      F  ++ P+S+
Sbjct: 250 RLCNKVAHSMANLSLSLSEERFWMEDCPASV 276

BLAST of Lcy01g006690 vs. NCBI nr
Match: XP_028960018.1 (uncharacterized protein LOC114825459 [Malus domestica])

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-11
Identity = 47/135 (34.81%), Postives = 72/135 (53.33%), Query Frame = 0

Query: 3    HGPLINCVKINVDVSWNKELKR-GVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRK 62
            H P    +KIN D +W+K  ++ G+GW+ CD  G   G G +   R  S  A E  AIR+
Sbjct: 1001 HKPGFGTIKINTDAAWSKVTQQAGLGWVACDFAGVLQGAGGSGSIRFHSAAAAEVAAIRE 1060

Query: 63   GL-DAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFV 122
             L D  L   R     VI+ESD+  +V+ + +E     + + ++ DIE LA  +  + F+
Sbjct: 1061 ALEDCLLRGFR----NVIIESDAKTIVQMIRNELTQDFRLECLLGDIEFLARRMQAVVFI 1120

Query: 123  KCPRASNKVAHVLTR 136
               R SNK AH++T+
Sbjct: 1121 FVSRESNKAAHLVTK 1131

BLAST of Lcy01g006690 vs. NCBI nr
Match: XP_028948099.1 (uncharacterized protein LOC114820925 [Malus domestica])

HSP 1 Score: 71.6 bits (174), Expect = 7.5e-09
Identity = 44/129 (34.11%), Postives = 67/129 (51.94%), Query Frame = 0

Query: 5   PLINCVKINVDVSWNKELK-RGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGL 64
           P    +K+N D +W KE K  GVGW++ D  G P  V     QR  +   +E  AIR+GL
Sbjct: 14  PSFGVLKLNCDAAWRKETKVGGVGWVLRDFAGLPNLVEGVGGQRFAATIMVEAEAIRQGL 73

Query: 65  DAYLSS-KREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKC 124
           +  +SS   EP   ++V SDS  +++ +N E +      + + DI  +A L  ++ F   
Sbjct: 74  EMVISSGVLEPGGSLVVASDSKGLIQMINQELMADVALDIYLQDIWRMANLFQLVRFCFT 133

Query: 125 PRASNKVAH 132
           PR  N+ AH
Sbjct: 134 PRQYNRAAH 142

BLAST of Lcy01g006690 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-08
Identity = 41/116 (35.34%), Postives = 67/116 (57.76%), Query Frame = 0

Query: 11  KINVDVSWNKEL-KRGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGLDAYLSS 70
           K+N   SW++E  K GV W+I DSLGS + +   +++R W +K LE  A+ +GL  YL  
Sbjct: 7   KLNSYASWSEEAGKGGVSWVIHDSLGSSICIECKELRRKWLVKMLEGKAMIEGLKTYLLI 66

Query: 71  KREPSF--RVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCP 124
           +   S+  R I+     ++ R LN+  +D  +   V+D+I +L  L G+I+F + P
Sbjct: 67  RETVSYSHRPII---FLELARILNNTHMDLIELSNVVDEIFDLELLAGIISFFQVP 119

BLAST of Lcy01g006690 vs. NCBI nr
Match: XP_021753633.1 (uncharacterized protein LOC110719026 [Chenopodium quinoa])

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-08
Identity = 40/127 (31.50%), Postives = 66/127 (51.97%), Query Frame = 0

Query: 5   PLINCVKINVDVSWNKELKRGVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGLD 64
           P +  +K+NVD  +  EL  G+G ++ D +G  + VG  +I+ +W ++  E  AI  GL 
Sbjct: 158 PAVGFLKVNVDNGFVGELACGLGAVVRDEVGIVVAVGVQQIKASWEVRIAEAKAIEWGLR 217

Query: 65  AYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCPR 124
                       ++VESD   V++AL ++  D S+  L++DDI  L +    + +    R
Sbjct: 218 VAAGLGVRD---LVVESDCLQVIQALKNKTADLSELSLIIDDILLLCSSFDNVIWSYVKR 277

Query: 125 ASNKVAH 132
             NKVAH
Sbjct: 278 GGNKVAH 281

BLAST of Lcy01g006690 vs. NCBI nr
Match: XP_021808158.1 (uncharacterized protein LOC110751913 [Prunus avium])

HSP 1 Score: 68.6 bits (166), Expect = 6.3e-08
Identity = 49/140 (35.00%), Postives = 73/140 (52.14%), Query Frame = 0

Query: 5   PLINCVKINVDVSWNKELKR-GVGWIICDSLGSPMGVGYAKIQRNWSIKALETLAIRKGL 64
           P  + +K+N DV+W  +L R GVGW+I DS G  +  G     R  S   +E LAIR  L
Sbjct: 79  PPPSLLKVNCDVAWFAQLLRGGVGWVIRDSHGLLLCAGGEGDLRGSSALLMELLAIRHAL 138

Query: 65  DAYLSSKREPSFRVIVESDSTDVVRALNHEWVDASKSKLVMDDIEELAALVGVIAFVKCP 124
            A    +      V+VESD+   +  LN +    S  + ++ DI++L A    ++FV  P
Sbjct: 139 SACAHFQLT---HVMVESDTQRGISMLNGQATVDSDLEGIVFDIQQLVATFSQVSFVFAP 198

Query: 125 RASNKVAHVLTRATVGFRSK 144
           ++ N+VAH +     GF SK
Sbjct: 199 KSCNRVAHTV----AGFVSK 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6P5S2T03.1e-0835.00uncharacterized protein LOC110751913 OS=Prunus avium OX=42229 GN=LOC110751913 PE... [more]
A0A6J5UV011.2e-0733.33Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
M7ZXZ04.4e-0732.09Laccase OS=Triticum urartu OX=4572 GN=TRIUR3_26891 PE=3 SV=1[more]
A0A6J5UAY25.8e-0732.81Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
A0A803L9N82.2e-0627.15Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_028960018.12.7e-1134.81uncharacterized protein LOC114825459 [Malus domestica][more]
XP_028948099.17.5e-0934.11uncharacterized protein LOC114820925 [Malus domestica][more]
KAG6599977.12.8e-0835.34hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_021753633.12.8e-0831.50uncharacterized protein LOC110719026 [Chenopodium quinoa][more]
XP_021808158.16.3e-0835.00uncharacterized protein LOC110751913 [Prunus avium][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 9..141
e-value: 8.5E-10
score: 40.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 13..135
e-value: 7.3E-17
score: 61.3
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 5..144
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 5..144
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 12..135
e-value: 1.36651E-15
score: 67.3392
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 9..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g006690.1Lcy01g006690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity