Lcy01g005480 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g005480
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionprotein NRT1/ PTR FAMILY 7.3-like
LocationChr01: 7678465 .. 7683454 (-)
RNA-Seq ExpressionLcy01g005480
SyntenyLcy01g005480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCAAAACTAGAGCTCAATGGCGCCGTTTCTAAAGGCAACGGTGAGCTTCAGGTAATGATGTTCCCTCATCATGGTTGCTTTGCTTTGGCTTTACTTCAAATTTCATTTTCATGATTTTGCCTCCATTGTTACACTTCGGTCAATTCGTGCTATGATTGCCCATTTGTGCATTTCTATCACTTTCTCTAGCTAAAAGTTCATTTTTATCCACAATAGGGCTTGCTTTCGACTGTTTGAGATGGTGAATTGAATAGCCTTCTTTTGTTTTTTCTATTACTTGGACAACATGTTTCATTATTATTTTGTTTCAATAATGCATTTGTTCTATGTTTTCTTATTAAAGTAATGATTTAGTCCCCACCGAAACTAAATTGGTACTTACTAAAATTGAAGTGATGATTTAGTTATTGTAATTTAGAATTTGTAATAATTTAGTTCTTATTGTGAAAAAATCTATTAAGGTGTAGTAAAGGTTTCTTAGTTATGTAGATTGATAGATCACTGAGGAACCAAATGTGTTTCTAGATACTAAGTGCCTTCATAAAAACCCACCTATCATAACTTTATACTTCTGAAAAGTTGTTTTGTGATGTTAAAGTATTTGATATAAGATAAAGTTTGCCGTAACCTATTGGCTTGGGCTTATAGGTTTAGCGGTGATTTAACATGGTATTAGAAGTGCAATCGGTCCTATATTTGAACCGTATTTCATTCTCAATTAATATTGATAACCATTTATTGGGTCTTGTGCTAATTTTCAAGCCCACATGTGATGAGAGGTAAGTTTTTGGGTTTATTGGTAATTTAACATGCTAATCTTTCTCAACAACTTCTCGTTTTTCAAAACAATGCGAAACTAAAGTAATCTGTTTTCCCCTTTTTATTTTCTACCAAACTTGTAGGAAATGAGGAAGAAATAATACAAAACAACAGAAGATCACTAGTTTCAGTGAATGCTATCATTAATGACCGTCATCTTAACCTAAGATGACACCATTTGAACTGTTATCTTCATCAAAAGTCAAAGGTTCTACAGTGGGTTGTATATATTTAGTTTAATCGCTAATGCTATAGCTTTTAATATATATTTTATGTAGCTTAAGTTTGATATGAGATGGACTTTTATAAAGGTTTAACAGTGCCTAAAATGTTGCAAAAGGTGGCAGATCTACATCAGTTGGAGAATGGCCAAGAAAGGATTCAGAGTTCATGCTCAGAAGAAGCTGCTTTGGCTGATCAAAATGAAAAAACAACGGCAAAAGGAAGAAAAGTTGGTGGGTGGAATTGTGGGATGCTGCTGCTAGGTAAAAAAACTCATTTTTGGTGATCATATTATAAAAAATCTGAGAATCTCAACCCAAATTTCATTTCAAGCTCGGCATTTTTATTTTCCAAGGGCTCGTTTGATAACATTCTTCCCGCTGTTTGTGACCATTTTGTGGGAAATATATGTTTATCATATTATAAACAGAGAAAACTGAATTAGCATTCTGGTTCTGCTTGGAGTTGTTTGGCAACATTCCCATTTCTCGGCTCCAATTTTACCAAAAGAGCCCTAAATTTTGTCTTTTCCCCCTCTAAATGTTTAGAATTTGGTTTGGTAACTGTTGCAGTGAATCAAGGATTCATCACACTGGCATTTGCTGGAGTTGAAGTGAATTTGGTTCTGTTTTCAAAGTCAGTGCTGAGGCAAACCAATGCTGAAGCAGCAAATACATTCAGCAGATGGATGGGAACCACTTATTGCTTCTCTTTGTTGGGTGCATTTCTCAGTGACTCATATTTGGGAAGATATCTCACTTGTATCATTTTTCAACTCGTCTTCATTGTTGTAAGTGAGCTTCTTCTCTTGTTCCCTATTCTGTTGCTTCTATGTTTTGAAATCGTTCTGATAACGACTTTGTGCACCTATATATATTGTTTTTGTTTTCAACTTTTAGAAATACTTTTGAAATCTCAACTAAATACAGCGTCTTGGTGGATATCCTTACATAGGAATTCTTTTTGTAATTACAGTTTGTTAATGATTATTAGCGATGGGAAGGAGTTGCTAATTTAGATTCTGGGCAAGGGCTCCCTCTGCCCTTGGCCCCTAGGCTGTATGCCTTTTTTTGTGATTAATATATATCTTTGATGTCTCTTGTCAAAAAAAAAAAAAAAATACAAGAACTTTTAAACATGGTTTCTATTCTTCTAGAAAAGATCATGGAAAATTGTTTAGTTGACCATTTATAATTTTTTTTTAATTGTTATCATGTGTGGTCTATTTCTTAGAATAAGTTTTCTGTGGGTAACCATGACCTTGAGTATGTAAAGGGTTACCAAATAACTTTAACCTTACAAATTACTCCATCACAAATCTGAAAAACAAGTTAAACAGTTGTTTGGCAGAATAAGTCACTAGACAATCTCCACTCATTTAGTTTTTGAAACTTGTTGGATTTCTTTAAGTGAAGTGTCAAGGGCCCACTTAGCATTATGTTCATGGTCCACCCCCACTAATCCTCGAGGAGGCTTGGCTCACCTCAAAGTCAACTTTGAAAGACAACTGGGACTTTGTAAGTAATTAAGTTTTCAACCTAAACATCTGAATCCCATAACACTTCAGATAATATTTCCAAACATCCCCAGTCCTTACCATTAGAGCATCCCTGGATAGCTTGAAAATTTTAACTAAGATTATTTCAACATTAGGTTATAAAACAAAGAAATGTCAATTAATTAGCTTAGAAAACTAAAAAAAAAAATCTTCATGAGATGAGATTGAGGTTATAACATTCTTTAGTAAATGGTAGTGTTAGAGAAGAATGATATTCATGCAATATGAACTAAGTTCATGCTCTCTTTTTGCAGGGTCTGATAGCACTTTCCCTATTAACTCACATATCTCTGCTCAAACCCAATGGTTGTGGCAGAATAGGCGAATTATGTGACCCTCATGGCTTAACCGAACTCTCAATATTTTACACATCGATCTATCTTATTGCCTTAGGGAATGGAGCCCCTGAGCCTGCATTGGCTACATTCGGTGCAGAGCAGTTTGACGAGAACGATCCTGAGGAAAATCGAGCCAAGACCTCGTTTTACAGCTACTTCTATGTGTCGATAAACATGGGGTGTTTGATTGCAGAAACAGTTTTGGTTTATATAGAGAACTTGGGGCATTGGATGGTGGGGTTTTGGATATGTGCTGTGAGTGGATTGCTAGCCTATCTTTTGTTATTGAGTGGAACTTCAAGATATAGACGGTCCAAACCATGTGGAAATCCTATATCAAGATTTTCTCAAGTTGTAATGGCTTCCATGAAAAAGATGAACCTTCAATTGCCTTCAAATGGACAAGGCCTTTATGAAGGGTTTGGGAAAGAGGGTACCGGGAGAATGCTACACACTGAAGGCTTCAAGTAAGTAAGGTTTTTAATTTTTTTTCATCATATTTAGATTTTTTTTTTTTTTGAAAAAATTAAATAAACATTATGTAGTTTTTTAAAAACAAACAATGGTTGGTATGAGAATAATTATAAATTTAGGGAAAATTGTCTCTGGAAGAATATTATCTCTTTGAGGGATGAGTTTCTTAAACACTGAGATGATTCATCTTCATTTTTCTTTTCTGTTAGTGTTGTCCAAGTAAAATCATCCTCTTTTTAGAAAACATTTTTGGAAATAAGTTAGTCTGAACAATCTTTTCTAAAAGTTTATTAAAAGTGTTTAAAAAATTATTATTGAAGTGTAAAGAGATTGAGCAGCTGTTTGGGAAATTGCTCTGAAAATAATTTTCTGTTATTTTAAAAACTGTTCTCGAAATTGTAGTGGAATAACTTGTAAATGATTTGAGGAAGAAAGTTTATATGTTAAAAAATAAATTATTTGTGCACATGCTCATAAATATATAAAAATATATATAAAACAAAACTATTTTGAAATGGCTGAGATCCTTCAATGATTAATGACATGTGCTCTTAAATATTTATTGTACTACTTAATGAATACTTCTTTGAATTATGCTTTGCTTACCTGGAGGTGGTTGTGATGTGATTGAAGCCTTAATAATCAAAATGCTGACTTAAAGCTTTTTACTTTTTTCTTTTTCTTTTTGAAATATTGCAGCTTTTTGGACAGAGCAGCCATTCTTACACCAGAGGAGATAAACTTGATATGCAACCAAGGCCAAACTCCAAATCCATGGCAAATTTGCCCCGTCACTCAGGTTGAAGAAGTCAAATGCATTTTGAGACTTCTTCCAGTCTGGTTATGCACTATTTTCTCCTCTGTTGTGTTCATTCAAATGCTCTCTCTTTTTGTTGAACAAGGAGCTGCAATGGACACCCTCGTCGCGTCAAACTTCCACATACCTCCAGCTAGCATGACCGCCTTCGACATTGTAAGCACGACAACATTCATCATGCTTTATGACAGACTCCTTGTTCCTTTATACATCAGAATCACAAAAAGAAACCCAAAACCACCAAATGAGCTACAAAGGATTGGGATCGGCCTAGCCATTGCAATAGTAGCACTCATAATTGCAGGCTTCGTTGAGCAAAAACGGTTGAGGCATGCAAGTGTAAGTGGTAAAGAAACAAGTTCTTTGAGTATTTTCTGGCAAACGCCGCAATATGTACTCGTGGGGGTCTCTGAAGCATTTGTGTATGTAGCTCAGATGGATTTTTTCACATCACAAATACCAGAAGGATTGAAAAGCTTGGGAATGGCACTTTCCATGTCTTCTACAGCAATGGGGAGTTACCTTGCAAGCCTGATTCTAACAATTGTAATGGCTATCACAAAATCTAATGGCAAGCCCGGTTGGGTTCCTCCAAACCTGAACGACGGTCATTTGGACAGATTTTTCTTCCTATCAGCCGCTCTGACTGCTCTTAACTTGGCCCTGTATGTATACTGTGCCAAACGGTATAAAGGTTCAACGATAGAGAAGCGAGACAGAGACCAAGAAGAGGAGCTTGATAGAGAGTTTAAGTAA

mRNA sequence

ATGGAGTCAAAACTAGAGCTCAATGGCGCCGTTTCTAAAGGCAACGGTGAGCTTCAGGTGGCAGATCTACATCAGTTGGAGAATGGCCAAGAAAGGATTCAGAGTTCATGCTCAGAAGAAGCTGCTTTGGCTGATCAAAATGAAAAAACAACGGCAAAAGGAAGAAAAGTTGGTGGGTGGAATTGTGGGATGCTGCTGCTAGTGAATCAAGGATTCATCACACTGGCATTTGCTGGAGTTGAAGTGAATTTGGTTCTGTTTTCAAAGTCAGTGCTGAGGCAAACCAATGCTGAAGCAGCAAATACATTCAGCAGATGGATGGGAACCACTTATTGCTTCTCTTTGTTGGGTGCATTTCTCAGTGACTCATATTTGGGAAGATATCTCACTTGTATCATTTTTCAACTCGTCTTCATTGTTGGTCTGATAGCACTTTCCCTATTAACTCACATATCTCTGCTCAAACCCAATGGTTGTGGCAGAATAGGCGAATTATGTGACCCTCATGGCTTAACCGAACTCTCAATATTTTACACATCGATCTATCTTATTGCCTTAGGGAATGGAGCCCCTGAGCCTGCATTGGCTACATTCGGTGCAGAGCAGTTTGACGAGAACGATCCTGAGGAAAATCGAGCCAAGACCTCGTTTTACAGCTACTTCTATGTGTCGATAAACATGGGGTGTTTGATTGCAGAAACAGTTTTGGTTTATATAGAGAACTTGGGGCATTGGATGGTGGGGTTTTGGATATGTGCTGTGAGTGGATTGCTAGCCTATCTTTTGTTATTGAGTGGAACTTCAAGATATAGACGGTCCAAACCATGTGGAAATCCTATATCAAGATTTTCTCAAGTTGTAATGGCTTCCATGAAAAAGATGAACCTTCAATTGCCTTCAAATGGACAAGGCCTTTATGAAGGGTTTGGGAAAGAGGGTACCGGGAGAATGCTACACACTGAAGGCTTCAACTTTTTGGACAGAGCAGCCATTCTTACACCAGAGGAGATAAACTTGATATGCAACCAAGGCCAAACTCCAAATCCATGGCAAATTTGCCCCGTCACTCAGGTTGAAGAAGTCAAATGCATTTTGAGACTTCTTCCAGTCTGGTTATGCACTATTTTCTCCTCTGTTGTGTTCATTCAAATGCTCTCTCTTTTTGTTGAACAAGGAGCTGCAATGGACACCCTCGTCGCGTCAAACTTCCACATACCTCCAGCTAGCATGACCGCCTTCGACATTGTAAGCACGACAACATTCATCATGCTTTATGACAGACTCCTTGTTCCTTTATACATCAGAATCACAAAAAGAAACCCAAAACCACCAAATGAGCTACAAAGGATTGGGATCGGCCTAGCCATTGCAATAGTAGCACTCATAATTGCAGGCTTCGTTGAGCAAAAACGGTTGAGGCATGCAAGTGTAAGTGGTAAAGAAACAAGTTCTTTGAGTATTTTCTGGCAAACGCCGCAATATGTACTCGTGGGGGTCTCTGAAGCATTTGTGTATGTAGCTCAGATGGATTTTTTCACATCACAAATACCAGAAGGATTGAAAAGCTTGGGAATGGCACTTTCCATGTCTTCTACAGCAATGGGGAGTTACCTTGCAAGCCTGATTCTAACAATTGTAATGGCTATCACAAAATCTAATGGCAAGCCCGGTTGGGTTCCTCCAAACCTGAACGACGGTCATTTGGACAGATTTTTCTTCCTATCAGCCGCTCTGACTGCTCTTAACTTGGCCCTGTATGTATACTGTGCCAAACGGTATAAAGGTTCAACGATAGAGAAGCGAGACAGAGACCAAGAAGAGGAGCTTGATAGAGAGTTTAAGTAA

Coding sequence (CDS)

ATGGAGTCAAAACTAGAGCTCAATGGCGCCGTTTCTAAAGGCAACGGTGAGCTTCAGGTGGCAGATCTACATCAGTTGGAGAATGGCCAAGAAAGGATTCAGAGTTCATGCTCAGAAGAAGCTGCTTTGGCTGATCAAAATGAAAAAACAACGGCAAAAGGAAGAAAAGTTGGTGGGTGGAATTGTGGGATGCTGCTGCTAGTGAATCAAGGATTCATCACACTGGCATTTGCTGGAGTTGAAGTGAATTTGGTTCTGTTTTCAAAGTCAGTGCTGAGGCAAACCAATGCTGAAGCAGCAAATACATTCAGCAGATGGATGGGAACCACTTATTGCTTCTCTTTGTTGGGTGCATTTCTCAGTGACTCATATTTGGGAAGATATCTCACTTGTATCATTTTTCAACTCGTCTTCATTGTTGGTCTGATAGCACTTTCCCTATTAACTCACATATCTCTGCTCAAACCCAATGGTTGTGGCAGAATAGGCGAATTATGTGACCCTCATGGCTTAACCGAACTCTCAATATTTTACACATCGATCTATCTTATTGCCTTAGGGAATGGAGCCCCTGAGCCTGCATTGGCTACATTCGGTGCAGAGCAGTTTGACGAGAACGATCCTGAGGAAAATCGAGCCAAGACCTCGTTTTACAGCTACTTCTATGTGTCGATAAACATGGGGTGTTTGATTGCAGAAACAGTTTTGGTTTATATAGAGAACTTGGGGCATTGGATGGTGGGGTTTTGGATATGTGCTGTGAGTGGATTGCTAGCCTATCTTTTGTTATTGAGTGGAACTTCAAGATATAGACGGTCCAAACCATGTGGAAATCCTATATCAAGATTTTCTCAAGTTGTAATGGCTTCCATGAAAAAGATGAACCTTCAATTGCCTTCAAATGGACAAGGCCTTTATGAAGGGTTTGGGAAAGAGGGTACCGGGAGAATGCTACACACTGAAGGCTTCAACTTTTTGGACAGAGCAGCCATTCTTACACCAGAGGAGATAAACTTGATATGCAACCAAGGCCAAACTCCAAATCCATGGCAAATTTGCCCCGTCACTCAGGTTGAAGAAGTCAAATGCATTTTGAGACTTCTTCCAGTCTGGTTATGCACTATTTTCTCCTCTGTTGTGTTCATTCAAATGCTCTCTCTTTTTGTTGAACAAGGAGCTGCAATGGACACCCTCGTCGCGTCAAACTTCCACATACCTCCAGCTAGCATGACCGCCTTCGACATTGTAAGCACGACAACATTCATCATGCTTTATGACAGACTCCTTGTTCCTTTATACATCAGAATCACAAAAAGAAACCCAAAACCACCAAATGAGCTACAAAGGATTGGGATCGGCCTAGCCATTGCAATAGTAGCACTCATAATTGCAGGCTTCGTTGAGCAAAAACGGTTGAGGCATGCAAGTGTAAGTGGTAAAGAAACAAGTTCTTTGAGTATTTTCTGGCAAACGCCGCAATATGTACTCGTGGGGGTCTCTGAAGCATTTGTGTATGTAGCTCAGATGGATTTTTTCACATCACAAATACCAGAAGGATTGAAAAGCTTGGGAATGGCACTTTCCATGTCTTCTACAGCAATGGGGAGTTACCTTGCAAGCCTGATTCTAACAATTGTAATGGCTATCACAAAATCTAATGGCAAGCCCGGTTGGGTTCCTCCAAACCTGAACGACGGTCATTTGGACAGATTTTTCTTCCTATCAGCCGCTCTGACTGCTCTTAACTTGGCCCTGTATGTATACTGTGCCAAACGGTATAAAGGTTCAACGATAGAGAAGCGAGACAGAGACCAAGAAGAGGAGCTTGATAGAGAGTTTAAGTAA

Protein sequence

MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQGLYEGFGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTTFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGKETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIEKRDRDQEEELDREFK
Homology
BLAST of Lcy01g005480 vs. ExPASy Swiss-Prot
Match: Q9LQL2 (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 568.9 bits (1465), Expect = 6.8e-161
Identity = 295/559 (52.77%), Postives = 400/559 (71.56%), Query Frame = 0

Query: 58  GGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLG 117
           G W  G+++L+NQG  TLAF GV VNLVLF   VL+Q NA+AAN  S+W GT Y FSL+G
Sbjct: 39  GQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVG 98

Query: 118 AFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIF 177
           AFLSDSY GRY TC IFQ++F++GL +LSL +++ L++P GCG     C  H + E+++F
Sbjct: 99  AFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMF 158

Query: 178 YTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLV 237
           Y SIYLIALG G  +P +AT GA+QFDE  P+E  +K +F+SYFY+++N+G L + T+L 
Sbjct: 159 YFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILG 218

Query: 238 YIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQ 297
           Y E+ G W +GFW    S ++  +L L GT RYR  KP GNP+SRF QV++A+ KK +++
Sbjct: 219 YFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVE 278

Query: 298 LPSNG-QGLYEG--FGKE---GTG-RMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPW 357
            P  G + +Y+G   GK     TG R++HT+ F FLD+AA +T  +++    +  + NPW
Sbjct: 279 APLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLD--DKKQDSVNPW 338

Query: 358 QICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASM 417
           ++CPVTQVEEVKCILRL+P+WLCTI  SVVF QM SLFVEQGAAM+T V S+F IPPASM
Sbjct: 339 RLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSV-SDFKIPPASM 398

Query: 418 TAFDIVSTTTFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQK 477
           ++FDI+S   FI LY R+L P+  R  K   K   EL R+GIGL IA++A+I AG VE  
Sbjct: 399 SSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECY 458

Query: 478 RLRHASVSGKE---TSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMAL 537
           RL++A  S      +SSLSIFWQ PQY L+G SE F+YV Q++FF +Q P+GLKS G AL
Sbjct: 459 RLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSAL 518

Query: 538 SMSSTAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYV 597
            M S +MG++++SL++T+V+ I+  +  PGW+P NLN GHLDRF+FL AALT+++L +Y+
Sbjct: 519 CMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYI 578

Query: 598 YCAKRYKGSTIEKRDRDQE 607
            CAK YK   +E +D  Q+
Sbjct: 579 ACAKWYKPIQLEGKDEMQD 594

BLAST of Lcy01g005480 vs. ExPASy Swiss-Prot
Match: Q8GXN2 (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 536.2 bits (1380), Expect = 4.9e-151
Identity = 286/544 (52.57%), Postives = 372/544 (68.38%), Query Frame = 0

Query: 58  GGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLG 117
           G W   +L+LVNQG  TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y FSLLG
Sbjct: 30  GKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDNAEAANNVSKWTGTVYIFSLLG 89

Query: 118 AFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIF 177
           AFLSDSY GRY TC IFQ  F+ GL+ LSL T   LL+P+GCG     C PH   +  +F
Sbjct: 90  AFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEPSGCGVEDSPCKPHSTFKTVLF 149

Query: 178 YTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLV 237
           Y S+YLIALG G  +P +ATFGA+QFD  D  E  +K +F+SYFY+++N+G L + TVL 
Sbjct: 150 YLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIAFFSYFYLALNLGSLFSNTVLG 209

Query: 238 YIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQ 297
           Y E+ G W +GFW  A S     +L L GT +YR   P  +P SRF QV++A+ +K  + 
Sbjct: 210 YFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPRESPWSRFCQVLVAATRKAKID 269

Query: 298 LPSNGQGLYEGFGK-EGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVT 357
           +      LY+   +  G  ++LHT+GF FLDRAAI+TP++       G   +PW++C VT
Sbjct: 270 VHHEELNLYDSETQYTGDKKILHTKGFRFLDRAAIVTPDDEAEKVESGSKYDPWRLCSVT 329

Query: 358 QVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIV 417
           QVEEVKC+LRLLP+WLCTI  SVVF QM SLFV QGAAM T +  NF IP +SM++FDI+
Sbjct: 330 QVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQGAAMKTNI-KNFRIPASSMSSFDIL 389

Query: 418 STTTFIMLYDRLLVPLYIRI--TKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRH 477
           S   FI  Y R L PL+ R+  T+RN K   ELQR+GIGL IAI+A+I AG VE  RL++
Sbjct: 390 SVAFFIFAYRRFLDPLFARLNKTERN-KGLTELQRMGIGLVIAIMAMISAGIVEIHRLKN 449

Query: 478 ----ASVSGKETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMS 537
               ++ S   +S+LSIFWQ PQY+L+G SE F+YV Q++FF SQ P GLKS   AL M+
Sbjct: 450 KEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQLEFFNSQAPTGLKSFASALCMA 509

Query: 538 STAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCA 595
           S ++G+Y++SL+++IVM I+ ++   GW+P NLN GHL+RF+FL A LTA +  +Y+ CA
Sbjct: 510 SISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHLERFYFLLAGLTAADFVVYLICA 569

BLAST of Lcy01g005480 vs. ExPASy Swiss-Prot
Match: Q3E9B5 (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 8.4e-143
Identity = 275/582 (47.25%), Postives = 384/582 (65.98%), Query Frame = 0

Query: 18  LQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGWNCGMLLLVNQGFITLAF 77
           L+V ++ ++++ Q  + S  S ++ L  Q +    + +K GGW   ++LLVNQG  TLAF
Sbjct: 24  LEVREVVEVQDDQS-VVSLMSNDSDL--QKKMMKKEEKKNGGWTNAIILLVNQGLATLAF 83

Query: 78  AGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCIIFQLV 137
            GV VNLVLF   V+ Q NAEAAN  S+W GT Y FSL+GAFLSDSY GRYLTC IFQ++
Sbjct: 84  FGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSLVGAFLSDSYWGRYLTCTIFQVI 143

Query: 138 FIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTSIYLIALGNGAPEPALAT 197
           F++G+  LS ++   L+KP GCG     C+P     ++IFY S+YL+A G G  +P LAT
Sbjct: 144 FVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVAIFYLSVYLVAFGYGGHQPTLAT 203

Query: 198 FGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGHWMVGFWICAVSGL 257
           FGA+Q D    ++  +K +F+SYFY ++N+G L + T+LVY E+ G W  GF +   S +
Sbjct: 204 FGADQLD----DDKNSKAAFFSYFYFALNVGALFSNTILVYFEDKGLWTEGFLVSLGSAI 263

Query: 258 LAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQGLYEGFGKE----G 317
           +A +  L+ T +YR  KPCGNP+ R +QV +A+ +K ++  P +   LYE  G E    G
Sbjct: 264 VALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWSVVRPGDPHELYELEGPESAIKG 323

Query: 318 TGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEEVKCILRLLPVWLC 377
           + ++ H+  F FLDRAA++T  + N     G   N W++C VTQVEE KC+++LLP+WLC
Sbjct: 324 SRKIFHSTKFLFLDRAAVITENDRN-----GTRSNAWRLCSVTQVEEAKCVMKLLPIWLC 383

Query: 378 TIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTTFIMLYDRLLVPLY 437
           TI  SV+F QM SLFVEQG  M+  V   FHIP ASM+ FDI S      +Y  ++ P Y
Sbjct: 384 TIIYSVIFTQMASLFVEQGDVMNAYV-GKFHIPAASMSVFDIFSVFVSTGIYRHIIFP-Y 443

Query: 438 IRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGKETSSLSIFWQTPQ 497
           +R        P EL R+GIGL I I+A++ AG  E +RL+   V G++ S L+I WQ PQ
Sbjct: 444 VR--------PTELMRMGIGLIIGIMAMVAAGLTEIQRLKRV-VPGQKESELTILWQIPQ 503

Query: 498 YVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLASLILTIVMAITK-S 557
           YVLVG SE F+YV Q++FF  Q P+GLK+LG +L M+S A+G+Y++SL++ IVMAITK  
Sbjct: 504 YVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMALGNYVSSLMVNIVMAITKRG 563

Query: 558 NGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYK 595
              PGW+P NLN+GH+DRF+FL AAL A++  +Y+  AK Y+
Sbjct: 564 ENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKWYQ 582

BLAST of Lcy01g005480 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.8e-119
Identity = 227/553 (41.05%), Postives = 351/553 (63.47%), Query Frame = 0

Query: 49  KTTAKGRKVGGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMG 108
           K  A   K G W     +L N+    LA+ G+  NLV + +S L Q NA AAN  + W G
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 109 TTYCFSLLGAFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDP 168
           T Y   L+GAF++D+YLGRY T   F  +++ G+  L+L   +  LKP  C    + C P
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--ADTCHP 136

Query: 169 HGLTELSIFYTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMG 228
           +  ++ ++F+ ++Y+IALG G  +P +++FGA+QFDEND  E   K+SF+++FY SIN+G
Sbjct: 137 NS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVG 196

Query: 229 CLIAETVLVYIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVM 288
            LIA TVLV+I+    W  GF +  V+ ++A      G+  YR  +P G+P++R  QV++
Sbjct: 197 ALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIV 256

Query: 289 ASMKKMNLQLPSNGQGLYEGFGKE----GTGRMLHTEGFNFLDRAAILTPEEINLICNQG 348
           A+ +K+++++P +   L+E    E    G+ +++HT+   F D+AA+   E  +     G
Sbjct: 257 AAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV---ESQSDSIKDG 316

Query: 349 QTPNPWQICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFH 408
           +  NPW++C VTQVEE+K I+ LLPVW   I  + V+ QM ++FV QG  MD  +  NF 
Sbjct: 317 EV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFE 376

Query: 409 IPPASMTAFDIVSTTTFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIA 468
           IP AS++ FD VS   +  +YD+ ++PL  + T RN +   +LQR+GIGL ++I A+I A
Sbjct: 377 IPSASLSLFDTVSVLFWTPVYDQFIIPLARKFT-RNERGFTQLQRMGIGLVVSIFAMITA 436

Query: 469 GFVEQKRLRHASVSG---KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLK 528
           G +E  RL +        ++   +SIFWQ PQY+L+G +E F ++ Q++FF  Q P+ ++
Sbjct: 437 GVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMR 496

Query: 529 SLGMALSMSSTAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTAL 588
           SL  ALS+++ A+G+YL+++++T+VM ITK NGKPGW+P NLN GHLD FF+L A L+ L
Sbjct: 497 SLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFL 556

Query: 589 NLALYVYCAKRYK 595
           N  +Y++ +KRYK
Sbjct: 557 NFLVYLWISKRYK 561

BLAST of Lcy01g005480 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 2.9e-111
Identity = 229/595 (38.49%), Postives = 355/595 (59.66%), Query Frame = 0

Query: 12  SKGNGELQVADLHQLENGQERIQ------SSCSEEAALADQNEKTTAKGRKVGGWNCGML 71
           S    E ++ D+ +    QE +       SS +E+ ++       + K  K G W     
Sbjct: 4   SNEEDERRILDVEESLLTQEEVNNHLTGLSSTAEDGSIDIYGNPPSKK--KTGNWKACPF 63

Query: 72  LLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYL 131
           +L N+    LA+ G+  NL+ +  S L ++N  AA+    W GT Y   L+GA ++DSY 
Sbjct: 64  ILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYW 123

Query: 132 GRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGC-GRIGELCDPHGLTELSIFYTSIYLI 191
           GRY T   F  ++ +G+  L+L   + +LKP  C G    LC P    + ++F+T +YLI
Sbjct: 124 GRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLI 183

Query: 192 ALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGH 251
           ALG G  +P +++FGA+QFD+ DP E   K SF+++FY SIN+G  I+ T+LV+++    
Sbjct: 184 ALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVG 243

Query: 252 WMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQG 311
           W +GF I  V   ++      GT  YR  KP G+PI+R  QV++A+ +K+ L LP +   
Sbjct: 244 WGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISF 303

Query: 312 LYEGFGKE----GTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEE 371
           LYE   K     G+ ++ HT+G+ FLD+AA+++  E       G   NPW++C VTQVEE
Sbjct: 304 LYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYE----SKSGAFSNPWKLCTVTQVEE 363

Query: 372 VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTT 431
           VK ++R+ P+W   I  SV++ Q+ +LFV+QG +M+ ++ S F IPPAS   FD +    
Sbjct: 364 VKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRIIRS-FEIPPASFGVFDTLIVLI 423

Query: 432 FIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGK 491
            I +YDR LVP   R T   PK   +LQR+GIGL ++++++  A  VE  RL+ A    +
Sbjct: 424 SIPIYDRFLVPFVRRFT-GIPKGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLA----Q 483

Query: 492 ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLAS 551
           +  ++SIFWQ PQY+L+G++E F ++ +++FF  + P+ ++S+  AL++ +TA+GSYL+S
Sbjct: 484 DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSS 543

Query: 552 LILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALY-VYCAKRYK 595
           LILT+V   T   GK GWVP +LN GHLD FF+L  +L  +N+ +Y + C K  K
Sbjct: 544 LILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTK 586

BLAST of Lcy01g005480 vs. ExPASy TrEMBL
Match: A0A6J1H418 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111460297 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 547/615 (88.94%), Postives = 581/615 (94.47%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK ELNG VS GN +LQVADLHQLENGQE  QSS SEEAALADQN+KTTAKGRKVGGW
Sbjct: 37  MESKPELNGTVSSGNDQLQVADLHQLENGQEGFQSSGSEEAALADQNDKTTAKGRKVGGW 96

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 97  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 156

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGL+ALSLLTHISLLKP+GCG+IG+LCDPHGLTEL+IFY S
Sbjct: 157 SDSYLGRYLTCVIFQLVFIVGLVALSLLTHISLLKPSGCGKIGQLCDPHGLTELTIFYIS 216

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+E RAKTSFYSYFYVSINMGCLI+ETVLVYIE
Sbjct: 217 IYLIALGNGAPEPALATFGAEQFDDKDPKEKRAKTSFYSYFYVSINMGCLISETVLVYIE 276

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTSRYR+SKP GNPISRFSQV+MASMKKMNL++PS
Sbjct: 277 NLGHWMVGFWICAVCGLLAYLLLLSGTSRYRQSKPSGNPISRFSQVIMASMKKMNLKMPS 336

Query: 301 NGQGLYEG-FGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           NG+GLYEG  GK+GT RMLHTEGF FLDRAAILT EEINLICNQGQT NPW++C V+QVE
Sbjct: 337 NGEGLYEGPGGKKGTRRMLHTEGFKFLDRAAILTEEEINLICNQGQTANPWKLCTVSQVE 396

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVWLCTIF+SVVFIQMLSLFVEQGAAM+TLV SNFHIPPASMTAFDIVSTT
Sbjct: 397 EVKCILRLLPVWLCTIFASVVFIQMLSLFVEQGAAMNTLVVSNFHIPPASMTAFDIVSTT 456

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVP YIRITKR PKPP+ELQRIGIGLAI +VAL+IAGFVEQ+RL++ASV+G
Sbjct: 457 LFIMLYDRLLVPFYIRITKRKPKPPSELQRIGIGLAITVVALVIAGFVEQRRLKYASVTG 516

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIP+GLKSLGM LSMSS+AMGSYLA
Sbjct: 517 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPDGLKSLGMGLSMSSSAMGSYLA 576

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           SLILT VMAITK+NGKPGWVPPNLNDGHLDRFFFLSA LTALNLALY+ CAKRYKGS IE
Sbjct: 577 SLILTTVMAITKTNGKPGWVPPNLNDGHLDRFFFLSATLTALNLALYLVCAKRYKGSIIE 636

Query: 601 KRDRDQEEELDREFK 615
           K+D D EEE D EFK
Sbjct: 637 KQDEDLEEEFDSEFK 651

BLAST of Lcy01g005480 vs. ExPASy TrEMBL
Match: A0A6J1L195 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111498952 PE=3 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 547/615 (88.94%), Postives = 577/615 (93.82%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK ELNG VS GN +LQVADLHQLENGQE  QSS SEEAALADQN KTTAKGRKVGGW
Sbjct: 38  MESKPELNGTVSSGNDQLQVADLHQLENGQEGFQSSGSEEAALADQNVKTTAKGRKVGGW 97

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 98  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 157

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGLIALSLLTHISLLKP+GCG+IG+LCDPHGLTEL IFY S
Sbjct: 158 SDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPSGCGKIGQLCDPHGLTELMIFYIS 217

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+E +AK SFYSYFYVSINMGCLIAETVLVYIE
Sbjct: 218 IYLIALGNGAPEPALATFGAEQFDDKDPKEKQAKASFYSYFYVSINMGCLIAETVLVYIE 277

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTS+YR+SKP GNPISRFSQV+MASMKKMNL++PS
Sbjct: 278 NLGHWMVGFWICAVCGLLAYLLLLSGTSKYRQSKPSGNPISRFSQVIMASMKKMNLKMPS 337

Query: 301 NGQGLYEG-FGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           NG+GLYEG  GKEGT RMLHTEGF FLDRAAILT EE NLICNQGQT NPW++C V+QVE
Sbjct: 338 NGEGLYEGPGGKEGTRRMLHTEGFKFLDRAAILTEEETNLICNQGQTANPWKLCTVSQVE 397

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVWLCTIF+SVVFIQMLSLFVEQGAAMDTLV SNFHIPPASMTAFDIVSTT
Sbjct: 398 EVKCILRLLPVWLCTIFASVVFIQMLSLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTT 457

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVPLYIRITKR PKPP+ELQRIGIGLAI +VAL+IAGFVEQ+RL++ASV+G
Sbjct: 458 LFIMLYDRLLVPLYIRITKRKPKPPSELQRIGIGLAITVVALVIAGFVEQRRLKYASVTG 517

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIP+GLKSLGM LSMSS+AMGSYLA
Sbjct: 518 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPDGLKSLGMGLSMSSSAMGSYLA 577

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           SLILT VMAITK+NGKPGWVPPNLNDGHLDRFFFLSA LTALNL LY+ CAKRYKGS IE
Sbjct: 578 SLILTTVMAITKTNGKPGWVPPNLNDGHLDRFFFLSATLTALNLVLYLVCAKRYKGSIIE 637

Query: 601 KRDRDQEEELDREFK 615
           K+D D EEE D EFK
Sbjct: 638 KQDEDLEEEFDSEFK 652

BLAST of Lcy01g005480 vs. ExPASy TrEMBL
Match: A0A6J1DSQ5 (protein NRT1/ PTR FAMILY 7.3-like isoform X5 OS=Momordica charantia OX=3673 GN=LOC111022811 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.9e-310
Identity = 540/615 (87.80%), Postives = 578/615 (93.98%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGE-LQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGG 60
           MES+ E++GA+S G+ E LQVADLHQLENGQER QSS +EEAALADQN+KTT KGR++GG
Sbjct: 1   MESEPEIDGAISSGDDELLQVADLHQLENGQERFQSSYTEEAALADQNDKTTPKGRRIGG 60

Query: 61  WNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF 120
           WNCGMLLLVNQG +TLA+AGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF
Sbjct: 61  WNCGMLLLVNQGLVTLAYAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF 120

Query: 121 LSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYT 180
           LSDSYLGRYLTCIIFQL+FIVGLIALSLLTHISLLKPNGCG+IG+LCDPHGLTELSIFY 
Sbjct: 121 LSDSYLGRYLTCIIFQLIFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHGLTELSIFYI 180

Query: 181 SIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYI 240
           SIYLIALGNGAPEPALATFGAEQFD+ DP+ENRAK SFYSYFYVSINMGCL +ETVLVY+
Sbjct: 181 SIYLIALGNGAPEPALATFGAEQFDDKDPKENRAKASFYSYFYVSINMGCLFSETVLVYM 240

Query: 241 ENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLP 300
           ENLGHW VGFWIC  S LL Y+LLLSG+SRYR+SKPCGNPISRFSQVVMASMKKMNL +P
Sbjct: 241 ENLGHWKVGFWICGGSALLGYILLLSGSSRYRQSKPCGNPISRFSQVVMASMKKMNLPMP 300

Query: 301 SNGQGLYEGFGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           SNG+GLYEG GKE T RMLHTEGF FLDRAAILT EE+NLI NQGQTPNPWQIC V+QVE
Sbjct: 301 SNGEGLYEGLGKEATRRMLHTEGFKFLDRAAILTAEEMNLIWNQGQTPNPWQICCVSQVE 360

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVW CTIFSSVVFIQMLSLFVEQGA+MDT+V SNFHIPPASMTAFDIVSTT
Sbjct: 361 EVKCILRLLPVWFCTIFSSVVFIQMLSLFVEQGASMDTVV-SNFHIPPASMTAFDIVSTT 420

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVP+YIRI KR PKPPNELQRIGIGLAIAIVAL+IAGFVEQKRLRHAS+SG
Sbjct: 421 LFIMLYDRLLVPIYIRIAKRRPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLRHASISG 480

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           +ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFF SQ P+GLKSLGM LSMSSTAMGSYLA
Sbjct: 481 EETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFASQTPDGLKSLGMGLSMSSTAMGSYLA 540

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           S+ILTIVMA+T++NGKPGWVPPNLNDGHLDRFFFLSAALTALNLA YV CAKRYKGST E
Sbjct: 541 SMILTIVMAVTETNGKPGWVPPNLNDGHLDRFFFLSAALTALNLAFYVICAKRYKGSTTE 600

Query: 601 KRDRDQEEELDREFK 615
           K+D D+EEELDREFK
Sbjct: 601 KQDGDREEELDREFK 614

BLAST of Lcy01g005480 vs. ExPASy TrEMBL
Match: A0A1S3CD14 (protein NRT1/ PTR FAMILY 7.3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499016 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 9.1e-310
Identity = 543/614 (88.44%), Postives = 569/614 (92.67%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK +L     K N ELQVADL  LENGQER Q S SE+ ALADQNEKT AK RKVGGW
Sbjct: 16  MESKPDL-----KSNDELQVADLQLLENGQERFQRSSSEDGALADQNEKTIAKRRKVGGW 75

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 76  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 135

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC IFQLVFIVGLI+LSLLTHISLLKPNGCG+IG+LCDPH LTELSIFY S
Sbjct: 136 SDSYLGRYLTCAIFQLVFIVGLISLSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYIS 195

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYL+ALGNGAPEPALATFGAEQFD+ DP+ENRAKTSFYSYFYVSINMGCLIAET+LVYIE
Sbjct: 196 IYLLALGNGAPEPALATFGAEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIE 255

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHW VGFWICAV GLLAYLL LSGTSRYR+SKPCGNPISRFSQV+MAS+KKMNL +PS
Sbjct: 256 NLGHWKVGFWICAVCGLLAYLLFLSGTSRYRQSKPCGNPISRFSQVLMASVKKMNLPMPS 315

Query: 301 NGQGLYEGFGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEE 360
           NG+GLYEG GKEGT RMLHTEGF FLDRAAILT EE NLICNQ QTPNPWQ+CPVTQVEE
Sbjct: 316 NGEGLYEGLGKEGTRRMLHTEGFKFLDRAAILTAEEANLICNQAQTPNPWQLCPVTQVEE 375

Query: 361 VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTT 420
           VKCILRLLPVWLCTIFSSVVFIQM+SLFVEQGAAMDTLV SNFHIPPASMTAFDIVSTT 
Sbjct: 376 VKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTM 435

Query: 421 FIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGK 480
           FIMLYDRLLVPLY+RI KR PKPPNELQRIGIGLAIAIVAL+IAGFVEQKRL+HA VSGK
Sbjct: 436 FIMLYDRLLVPLYVRIAKRKPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGK 495

Query: 481 ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLAS 540
           E SSLSIFWQTPQYVLVGV+EAFV+VAQMDFFTSQIPEGLKSLGM LSMSSTAMGSY+AS
Sbjct: 496 EISSLSIFWQTPQYVLVGVAEAFVFVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVAS 555

Query: 541 LILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIEK 600
           LILT VMAITK+NG PGWVPPNLNDGHLDRFFFLSAALTA NLALY+ CAK+YKGS IEK
Sbjct: 556 LILTTVMAITKTNGHPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEK 615

Query: 601 RDRDQEEELDREFK 615
           +D DQEEE DREFK
Sbjct: 616 QDGDQEEESDREFK 624

BLAST of Lcy01g005480 vs. ExPASy TrEMBL
Match: A0A5A7T9M4 (Protein NRT1/ PTR FAMILY 7.3-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00530 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.6e-306
Identity = 533/596 (89.43%), Postives = 559/596 (93.79%), Query Frame = 0

Query: 19  QVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGWNCGMLLLVNQGFITLAFA 78
           +VADL  LENGQER Q S SE+ ALADQNEKT AK RKVGGWNCGMLLLVNQG ITLAFA
Sbjct: 4   KVADLQLLENGQERFQRSSSEDGALADQNEKTIAKRRKVGGWNCGMLLLVNQGLITLAFA 63

Query: 79  GVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCIIFQLVF 138
           GVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTC IFQLVF
Sbjct: 64  GVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCAIFQLVF 123

Query: 139 IVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTSIYLIALGNGAPEPALATF 198
           IVGLI+LSLLTHISLLKPNGCG+IG+LCDPH LTELSIFY SIYL+ALGNGAPEPALATF
Sbjct: 124 IVGLISLSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLLALGNGAPEPALATF 183

Query: 199 GAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGHWMVGFWICAVSGLL 258
           GAEQFD+ DP+ENRAKTSFYSYFYVSINMGCLIAET+LVYIENLGHW VGFWICAV GLL
Sbjct: 184 GAEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWKVGFWICAVCGLL 243

Query: 259 AYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQGLYEGFGKEGTGRML 318
           AYLL LSGTSRYR+SKPCGNPISRFSQV+MAS+KKMNL +PSNG+GLYEG GKEGT RML
Sbjct: 244 AYLLFLSGTSRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKEGTRRML 303

Query: 319 HTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEEVKCILRLLPVWLCTIFSS 378
           HTEGF FLDRAAILT EE NLICNQ QTPNPWQ+CPVTQVEEVKCILRLLPVWLCTIFSS
Sbjct: 304 HTEGFKFLDRAAILTAEEANLICNQAQTPNPWQLCPVTQVEEVKCILRLLPVWLCTIFSS 363

Query: 379 VVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTTFIMLYDRLLVPLYIRITK 438
           VVFIQM+SLFVEQGAAMDTLV SNFHIPPASMTAFDIVSTT FIMLYDRLLVPLY+RI K
Sbjct: 364 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 423

Query: 439 RNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGKETSSLSIFWQTPQYVLVG 498
           R PKPPNELQRIGIGLAIAIVAL+IAGFVEQKRL+HA VSGKE SSLSIFWQTPQYVLVG
Sbjct: 424 RKPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSLSIFWQTPQYVLVG 483

Query: 499 VSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLASLILTIVMAITKSNGKPGW 558
           V+EAFV+VAQMDFFTSQIPEGLKSLGM LSMSSTAMGSY+ASLILT VMAITK+NG PGW
Sbjct: 484 VAEAFVFVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGHPGW 543

Query: 559 VPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIEKRDRDQEEELDREFK 615
           VPPNLNDGHLDRFFFLSAALTA NLALY+ CAK+YKGS IEK+D DQEEE DREFK
Sbjct: 544 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEESDREFK 599

BLAST of Lcy01g005480 vs. NCBI nr
Match: XP_038875638.1 (protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 561/614 (91.37%), Postives = 580/614 (94.46%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK EL       + ELQVADLH LENGQER QSS SE+AALADQN+KTTAKGRKVGGW
Sbjct: 1   MESKPELKA----NDEELQVADLHLLENGQERSQSSSSEDAALADQNDKTTAKGRKVGGW 60

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 61  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGLIALSLLTHISLLKPNGCG+IG+LCDPHGLTELSIFY S
Sbjct: 121 SDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHGLTELSIFYIS 180

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+ENRAKTSFYSYFYVSINMGCLIAETVLVYIE
Sbjct: 181 IYLIALGNGAPEPALATFGAEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTSRYR+SKPCGNPISRFSQVVMASMKKMNLQ+PS
Sbjct: 241 NLGHWMVGFWICAVCGLLAYLLLLSGTSRYRQSKPCGNPISRFSQVVMASMKKMNLQMPS 300

Query: 301 NGQGLYEGFGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEE 360
           NG+GLYE  GKEGT RM+HTEGF FLDRAAILT EEINLICNQ QTPNPWQICPVTQVEE
Sbjct: 301 NGEGLYEELGKEGTRRMVHTEGFKFLDRAAILTAEEINLICNQSQTPNPWQICPVTQVEE 360

Query: 361 VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTT 420
           VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDT+V SNFHIPPASMTAFDIVSTT 
Sbjct: 361 VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTIVVSNFHIPPASMTAFDIVSTTM 420

Query: 421 FIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGK 480
           FIMLYDRL+VPLYIRI KR PKPPNELQRIGIGLAIAIVAL+IAGFVEQKRL+HASVSGK
Sbjct: 421 FIMLYDRLIVPLYIRIAKRKPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLKHASVSGK 480

Query: 481 ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLAS 540
           ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGM LSMSS+AMGSYLAS
Sbjct: 481 ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSSAMGSYLAS 540

Query: 541 LILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIEK 600
           LILT VMAITK+NGK GWVPPNLNDGHLDRFFFLSAALTALNLA YV CAKRYKGS IEK
Sbjct: 541 LILTTVMAITKTNGKLGWVPPNLNDGHLDRFFFLSAALTALNLAFYVVCAKRYKGSVIEK 600

Query: 601 RDRDQEEELDREFK 615
           RD DQEEEL+ EFK
Sbjct: 601 RDGDQEEELNIEFK 610

BLAST of Lcy01g005480 vs. NCBI nr
Match: XP_022959267.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita moschata] >KAG6574799.1 Protein NRT1/ PTR FAMILY 7.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 547/615 (88.94%), Postives = 581/615 (94.47%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK ELNG VS GN +LQVADLHQLENGQE  QSS SEEAALADQN+KTTAKGRKVGGW
Sbjct: 37  MESKPELNGTVSSGNDQLQVADLHQLENGQEGFQSSGSEEAALADQNDKTTAKGRKVGGW 96

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 97  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 156

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGL+ALSLLTHISLLKP+GCG+IG+LCDPHGLTEL+IFY S
Sbjct: 157 SDSYLGRYLTCVIFQLVFIVGLVALSLLTHISLLKPSGCGKIGQLCDPHGLTELTIFYIS 216

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+E RAKTSFYSYFYVSINMGCLI+ETVLVYIE
Sbjct: 217 IYLIALGNGAPEPALATFGAEQFDDKDPKEKRAKTSFYSYFYVSINMGCLISETVLVYIE 276

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTSRYR+SKP GNPISRFSQV+MASMKKMNL++PS
Sbjct: 277 NLGHWMVGFWICAVCGLLAYLLLLSGTSRYRQSKPSGNPISRFSQVIMASMKKMNLKMPS 336

Query: 301 NGQGLYEG-FGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           NG+GLYEG  GK+GT RMLHTEGF FLDRAAILT EEINLICNQGQT NPW++C V+QVE
Sbjct: 337 NGEGLYEGPGGKKGTRRMLHTEGFKFLDRAAILTEEEINLICNQGQTANPWKLCTVSQVE 396

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVWLCTIF+SVVFIQMLSLFVEQGAAM+TLV SNFHIPPASMTAFDIVSTT
Sbjct: 397 EVKCILRLLPVWLCTIFASVVFIQMLSLFVEQGAAMNTLVVSNFHIPPASMTAFDIVSTT 456

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVP YIRITKR PKPP+ELQRIGIGLAI +VAL+IAGFVEQ+RL++ASV+G
Sbjct: 457 LFIMLYDRLLVPFYIRITKRKPKPPSELQRIGIGLAITVVALVIAGFVEQRRLKYASVTG 516

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIP+GLKSLGM LSMSS+AMGSYLA
Sbjct: 517 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPDGLKSLGMGLSMSSSAMGSYLA 576

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           SLILT VMAITK+NGKPGWVPPNLNDGHLDRFFFLSA LTALNLALY+ CAKRYKGS IE
Sbjct: 577 SLILTTVMAITKTNGKPGWVPPNLNDGHLDRFFFLSATLTALNLALYLVCAKRYKGSIIE 636

Query: 601 KRDRDQEEELDREFK 615
           K+D D EEE D EFK
Sbjct: 637 KQDEDLEEEFDSEFK 651

BLAST of Lcy01g005480 vs. NCBI nr
Match: XP_023006114.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 547/615 (88.94%), Postives = 577/615 (93.82%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK ELNG VS GN +LQVADLHQLENGQE  QSS SEEAALADQN KTTAKGRKVGGW
Sbjct: 38  MESKPELNGTVSSGNDQLQVADLHQLENGQEGFQSSGSEEAALADQNVKTTAKGRKVGGW 97

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 98  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 157

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGLIALSLLTHISLLKP+GCG+IG+LCDPHGLTEL IFY S
Sbjct: 158 SDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPSGCGKIGQLCDPHGLTELMIFYIS 217

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+E +AK SFYSYFYVSINMGCLIAETVLVYIE
Sbjct: 218 IYLIALGNGAPEPALATFGAEQFDDKDPKEKQAKASFYSYFYVSINMGCLIAETVLVYIE 277

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTS+YR+SKP GNPISRFSQV+MASMKKMNL++PS
Sbjct: 278 NLGHWMVGFWICAVCGLLAYLLLLSGTSKYRQSKPSGNPISRFSQVIMASMKKMNLKMPS 337

Query: 301 NGQGLYEG-FGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           NG+GLYEG  GKEGT RMLHTEGF FLDRAAILT EE NLICNQGQT NPW++C V+QVE
Sbjct: 338 NGEGLYEGPGGKEGTRRMLHTEGFKFLDRAAILTEEETNLICNQGQTANPWKLCTVSQVE 397

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVWLCTIF+SVVFIQMLSLFVEQGAAMDTLV SNFHIPPASMTAFDIVSTT
Sbjct: 398 EVKCILRLLPVWLCTIFASVVFIQMLSLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTT 457

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVPLYIRITKR PKPP+ELQRIGIGLAI +VAL+IAGFVEQ+RL++ASV+G
Sbjct: 458 LFIMLYDRLLVPLYIRITKRKPKPPSELQRIGIGLAITVVALVIAGFVEQRRLKYASVTG 517

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIP+GLKSLGM LSMSS+AMGSYLA
Sbjct: 518 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPDGLKSLGMGLSMSSSAMGSYLA 577

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           SLILT VMAITK+NGKPGWVPPNLNDGHLDRFFFLSA LTALNL LY+ CAKRYKGS IE
Sbjct: 578 SLILTTVMAITKTNGKPGWVPPNLNDGHLDRFFFLSATLTALNLVLYLVCAKRYKGSIIE 637

Query: 601 KRDRDQEEELDREFK 615
           K+D D EEE D EFK
Sbjct: 638 KQDEDLEEEFDSEFK 652

BLAST of Lcy01g005480 vs. NCBI nr
Match: XP_023548509.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.7e-310
Identity = 543/615 (88.29%), Postives = 577/615 (93.82%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGELQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGW 60
           MESK EL+G VS GN +LQVADLHQLENGQE  QSS SEEAALADQN KTTAKGRKVGGW
Sbjct: 37  MESKPELHGTVSSGNDQLQVADLHQLENGQEGFQSSGSEEAALADQNVKTTAKGRKVGGW 96

Query: 61  NCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 120
           NCGMLLLVNQG ITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL
Sbjct: 97  NCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFL 156

Query: 121 SDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTS 180
           SDSYLGRYLTC+IFQLVFIVGL+ALSLLTHISLLKP+GCG+IG+LCDPHGLTEL+IFY S
Sbjct: 157 SDSYLGRYLTCVIFQLVFIVGLVALSLLTHISLLKPSGCGKIGQLCDPHGLTELAIFYIS 216

Query: 181 IYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIE 240
           IYLIALGNGAPEPALATFGAEQFD+ DP+E RAKTSFYSYFYVSINMGCLI+ETVLVYIE
Sbjct: 217 IYLIALGNGAPEPALATFGAEQFDDKDPKEKRAKTSFYSYFYVSINMGCLISETVLVYIE 276

Query: 241 NLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPS 300
           NLGHWMVGFWICAV GLLAYLLLLSGTSRYR+SKP GNPISRFSQV+MASMKKMNL++PS
Sbjct: 277 NLGHWMVGFWICAVCGLLAYLLLLSGTSRYRQSKPSGNPISRFSQVIMASMKKMNLKMPS 336

Query: 301 NGQGLYEG-FGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           NG+GLYEG  GK+GT RMLHTEGF FLDRAAILT EEINLICNQGQT NPW++C V+QVE
Sbjct: 337 NGEGLYEGPGGKKGTRRMLHTEGFKFLDRAAILTEEEINLICNQGQTANPWKLCTVSQVE 396

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVWLCTIF+SVVFIQMLSLFVEQGAAMDTLV SNFHIPPASMTAFDIVSTT
Sbjct: 397 EVKCILRLLPVWLCTIFASVVFIQMLSLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTT 456

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVPLYIRI KR PKPP+ELQRIGIGLAI +VAL+IAGFVEQ+RL++ASV+G
Sbjct: 457 LFIMLYDRLLVPLYIRIAKRKPKPPSELQRIGIGLAITVVALVIAGFVEQRRLKYASVTG 516

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           KETSSLSIFWQTPQYVLVG+SEAFVYVAQMDFFTSQIP+GLKSLGM LSMSS+AMGSYLA
Sbjct: 517 KETSSLSIFWQTPQYVLVGISEAFVYVAQMDFFTSQIPDGLKSLGMGLSMSSSAMGSYLA 576

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           SLILT VMAITK+NGKPGWVPPNLNDGHLDRFFFLSA LTALNL  Y+ CAKRYKGS IE
Sbjct: 577 SLILTTVMAITKTNGKPGWVPPNLNDGHLDRFFFLSATLTALNLVFYLVCAKRYKGSIIE 636

Query: 601 KRDRDQEEELDREFK 615
            +D D EEE D EFK
Sbjct: 637 NQDEDLEEEFDSEFK 651

BLAST of Lcy01g005480 vs. NCBI nr
Match: XP_022155771.1 (protein NRT1/ PTR FAMILY 7.3-like isoform X5 [Momordica charantia])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.4e-309
Identity = 540/615 (87.80%), Postives = 578/615 (93.98%), Query Frame = 0

Query: 1   MESKLELNGAVSKGNGE-LQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGG 60
           MES+ E++GA+S G+ E LQVADLHQLENGQER QSS +EEAALADQN+KTT KGR++GG
Sbjct: 1   MESEPEIDGAISSGDDELLQVADLHQLENGQERFQSSYTEEAALADQNDKTTPKGRRIGG 60

Query: 61  WNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF 120
           WNCGMLLLVNQG +TLA+AGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF
Sbjct: 61  WNCGMLLLVNQGLVTLAYAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAF 120

Query: 121 LSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYT 180
           LSDSYLGRYLTCIIFQL+FIVGLIALSLLTHISLLKPNGCG+IG+LCDPHGLTELSIFY 
Sbjct: 121 LSDSYLGRYLTCIIFQLIFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHGLTELSIFYI 180

Query: 181 SIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYI 240
           SIYLIALGNGAPEPALATFGAEQFD+ DP+ENRAK SFYSYFYVSINMGCL +ETVLVY+
Sbjct: 181 SIYLIALGNGAPEPALATFGAEQFDDKDPKENRAKASFYSYFYVSINMGCLFSETVLVYM 240

Query: 241 ENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLP 300
           ENLGHW VGFWIC  S LL Y+LLLSG+SRYR+SKPCGNPISRFSQVVMASMKKMNL +P
Sbjct: 241 ENLGHWKVGFWICGGSALLGYILLLSGSSRYRQSKPCGNPISRFSQVVMASMKKMNLPMP 300

Query: 301 SNGQGLYEGFGKEGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVE 360
           SNG+GLYEG GKE T RMLHTEGF FLDRAAILT EE+NLI NQGQTPNPWQIC V+QVE
Sbjct: 301 SNGEGLYEGLGKEATRRMLHTEGFKFLDRAAILTAEEMNLIWNQGQTPNPWQICCVSQVE 360

Query: 361 EVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTT 420
           EVKCILRLLPVW CTIFSSVVFIQMLSLFVEQGA+MDT+V SNFHIPPASMTAFDIVSTT
Sbjct: 361 EVKCILRLLPVWFCTIFSSVVFIQMLSLFVEQGASMDTVV-SNFHIPPASMTAFDIVSTT 420

Query: 421 TFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSG 480
            FIMLYDRLLVP+YIRI KR PKPPNELQRIGIGLAIAIVAL+IAGFVEQKRLRHAS+SG
Sbjct: 421 LFIMLYDRLLVPIYIRIAKRRPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLRHASISG 480

Query: 481 KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLA 540
           +ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFF SQ P+GLKSLGM LSMSSTAMGSYLA
Sbjct: 481 EETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFASQTPDGLKSLGMGLSMSSTAMGSYLA 540

Query: 541 SLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYKGSTIE 600
           S+ILTIVMA+T++NGKPGWVPPNLNDGHLDRFFFLSAALTALNLA YV CAKRYKGST E
Sbjct: 541 SMILTIVMAVTETNGKPGWVPPNLNDGHLDRFFFLSAALTALNLAFYVICAKRYKGSTTE 600

Query: 601 KRDRDQEEELDREFK 615
           K+D D+EEELDREFK
Sbjct: 601 KQDGDREEELDREFK 614

BLAST of Lcy01g005480 vs. TAIR 10
Match: AT1G32450.1 (nitrate transporter 1.5 )

HSP 1 Score: 568.9 bits (1465), Expect = 4.8e-162
Identity = 295/559 (52.77%), Postives = 400/559 (71.56%), Query Frame = 0

Query: 58  GGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLG 117
           G W  G+++L+NQG  TLAF GV VNLVLF   VL+Q NA+AAN  S+W GT Y FSL+G
Sbjct: 39  GQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVG 98

Query: 118 AFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIF 177
           AFLSDSY GRY TC IFQ++F++GL +LSL +++ L++P GCG     C  H + E+++F
Sbjct: 99  AFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMF 158

Query: 178 YTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLV 237
           Y SIYLIALG G  +P +AT GA+QFDE  P+E  +K +F+SYFY+++N+G L + T+L 
Sbjct: 159 YFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILG 218

Query: 238 YIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQ 297
           Y E+ G W +GFW    S ++  +L L GT RYR  KP GNP+SRF QV++A+ KK +++
Sbjct: 219 YFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVE 278

Query: 298 LPSNG-QGLYEG--FGKE---GTG-RMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPW 357
            P  G + +Y+G   GK     TG R++HT+ F FLD+AA +T  +++    +  + NPW
Sbjct: 279 APLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLD--DKKQDSVNPW 338

Query: 358 QICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASM 417
           ++CPVTQVEEVKCILRL+P+WLCTI  SVVF QM SLFVEQGAAM+T V S+F IPPASM
Sbjct: 339 RLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSV-SDFKIPPASM 398

Query: 418 TAFDIVSTTTFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQK 477
           ++FDI+S   FI LY R+L P+  R  K   K   EL R+GIGL IA++A+I AG VE  
Sbjct: 399 SSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECY 458

Query: 478 RLRHASVSGKE---TSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMAL 537
           RL++A  S      +SSLSIFWQ PQY L+G SE F+YV Q++FF +Q P+GLKS G AL
Sbjct: 459 RLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSAL 518

Query: 538 SMSSTAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYV 597
            M S +MG++++SL++T+V+ I+  +  PGW+P NLN GHLDRF+FL AALT+++L +Y+
Sbjct: 519 CMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYI 578

Query: 598 YCAKRYKGSTIEKRDRDQE 607
            CAK YK   +E +D  Q+
Sbjct: 579 ACAKWYKPIQLEGKDEMQD 594

BLAST of Lcy01g005480 vs. TAIR 10
Match: AT4G21680.1 (NITRATE TRANSPORTER 1.8 )

HSP 1 Score: 536.2 bits (1380), Expect = 3.5e-152
Identity = 286/544 (52.57%), Postives = 372/544 (68.38%), Query Frame = 0

Query: 58  GGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLG 117
           G W   +L+LVNQG  TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y FSLLG
Sbjct: 30  GKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDNAEAANNVSKWTGTVYIFSLLG 89

Query: 118 AFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIF 177
           AFLSDSY GRY TC IFQ  F+ GL+ LSL T   LL+P+GCG     C PH   +  +F
Sbjct: 90  AFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEPSGCGVEDSPCKPHSTFKTVLF 149

Query: 178 YTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLV 237
           Y S+YLIALG G  +P +ATFGA+QFD  D  E  +K +F+SYFY+++N+G L + TVL 
Sbjct: 150 YLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIAFFSYFYLALNLGSLFSNTVLG 209

Query: 238 YIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQ 297
           Y E+ G W +GFW  A S     +L L GT +YR   P  +P SRF QV++A+ +K  + 
Sbjct: 210 YFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPRESPWSRFCQVLVAATRKAKID 269

Query: 298 LPSNGQGLYEGFGK-EGTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVT 357
           +      LY+   +  G  ++LHT+GF FLDRAAI+TP++       G   +PW++C VT
Sbjct: 270 VHHEELNLYDSETQYTGDKKILHTKGFRFLDRAAIVTPDDEAEKVESGSKYDPWRLCSVT 329

Query: 358 QVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIV 417
           QVEEVKC+LRLLP+WLCTI  SVVF QM SLFV QGAAM T +  NF IP +SM++FDI+
Sbjct: 330 QVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQGAAMKTNI-KNFRIPASSMSSFDIL 389

Query: 418 STTTFIMLYDRLLVPLYIRI--TKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRH 477
           S   FI  Y R L PL+ R+  T+RN K   ELQR+GIGL IAI+A+I AG VE  RL++
Sbjct: 390 SVAFFIFAYRRFLDPLFARLNKTERN-KGLTELQRMGIGLVIAIMAMISAGIVEIHRLKN 449

Query: 478 ----ASVSGKETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMS 537
               ++ S   +S+LSIFWQ PQY+L+G SE F+YV Q++FF SQ P GLKS   AL M+
Sbjct: 450 KEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQLEFFNSQAPTGLKSFASALCMA 509

Query: 538 STAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCA 595
           S ++G+Y++SL+++IVM I+ ++   GW+P NLN GHL+RF+FL A LTA +  +Y+ CA
Sbjct: 510 SISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHLERFYFLLAGLTAADFVVYLICA 569

BLAST of Lcy01g005480 vs. TAIR 10
Match: AT5G19640.1 (Major facilitator superfamily protein )

HSP 1 Score: 508.8 bits (1309), Expect = 6.0e-144
Identity = 275/582 (47.25%), Postives = 384/582 (65.98%), Query Frame = 0

Query: 18  LQVADLHQLENGQERIQSSCSEEAALADQNEKTTAKGRKVGGWNCGMLLLVNQGFITLAF 77
           L+V ++ ++++ Q  + S  S ++ L  Q +    + +K GGW   ++LLVNQG  TLAF
Sbjct: 24  LEVREVVEVQDDQS-VVSLMSNDSDL--QKKMMKKEEKKNGGWTNAIILLVNQGLATLAF 83

Query: 78  AGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCIIFQLV 137
            GV VNLVLF   V+ Q NAEAAN  S+W GT Y FSL+GAFLSDSY GRYLTC IFQ++
Sbjct: 84  FGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSLVGAFLSDSYWGRYLTCTIFQVI 143

Query: 138 FIVGLIALSLLTHISLLKPNGCGRIGELCDPHGLTELSIFYTSIYLIALGNGAPEPALAT 197
           F++G+  LS ++   L+KP GCG     C+P     ++IFY S+YL+A G G  +P LAT
Sbjct: 144 FVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVAIFYLSVYLVAFGYGGHQPTLAT 203

Query: 198 FGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGHWMVGFWICAVSGL 257
           FGA+Q D    ++  +K +F+SYFY ++N+G L + T+LVY E+ G W  GF +   S +
Sbjct: 204 FGADQLD----DDKNSKAAFFSYFYFALNVGALFSNTILVYFEDKGLWTEGFLVSLGSAI 263

Query: 258 LAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQGLYEGFGKE----G 317
           +A +  L+ T +YR  KPCGNP+ R +QV +A+ +K ++  P +   LYE  G E    G
Sbjct: 264 VALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWSVVRPGDPHELYELEGPESAIKG 323

Query: 318 TGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEEVKCILRLLPVWLC 377
           + ++ H+  F FLDRAA++T  + N     G   N W++C VTQVEE KC+++LLP+WLC
Sbjct: 324 SRKIFHSTKFLFLDRAAVITENDRN-----GTRSNAWRLCSVTQVEEAKCVMKLLPIWLC 383

Query: 378 TIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTTFIMLYDRLLVPLY 437
           TI  SV+F QM SLFVEQG  M+  V   FHIP ASM+ FDI S      +Y  ++ P Y
Sbjct: 384 TIIYSVIFTQMASLFVEQGDVMNAYV-GKFHIPAASMSVFDIFSVFVSTGIYRHIIFP-Y 443

Query: 438 IRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGKETSSLSIFWQTPQ 497
           +R        P EL R+GIGL I I+A++ AG  E +RL+   V G++ S L+I WQ PQ
Sbjct: 444 VR--------PTELMRMGIGLIIGIMAMVAAGLTEIQRLKRV-VPGQKESELTILWQIPQ 503

Query: 498 YVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLASLILTIVMAITK-S 557
           YVLVG SE F+YV Q++FF  Q P+GLK+LG +L M+S A+G+Y++SL++ IVMAITK  
Sbjct: 504 YVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMALGNYVSSLMVNIVMAITKRG 563

Query: 558 NGKPGWVPPNLNDGHLDRFFFLSAALTALNLALYVYCAKRYK 595
              PGW+P NLN+GH+DRF+FL AAL A++  +Y+  AK Y+
Sbjct: 564 ENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKWYQ 582

BLAST of Lcy01g005480 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 430.3 bits (1105), Expect = 2.7e-120
Identity = 227/553 (41.05%), Postives = 351/553 (63.47%), Query Frame = 0

Query: 49  KTTAKGRKVGGWNCGMLLLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMG 108
           K  A   K G W     +L N+    LA+ G+  NLV + +S L Q NA AAN  + W G
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 109 TTYCFSLLGAFLSDSYLGRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGCGRIGELCDP 168
           T Y   L+GAF++D+YLGRY T   F  +++ G+  L+L   +  LKP  C    + C P
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--ADTCHP 136

Query: 169 HGLTELSIFYTSIYLIALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMG 228
           +  ++ ++F+ ++Y+IALG G  +P +++FGA+QFDEND  E   K+SF+++FY SIN+G
Sbjct: 137 NS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVG 196

Query: 229 CLIAETVLVYIENLGHWMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVM 288
            LIA TVLV+I+    W  GF +  V+ ++A      G+  YR  +P G+P++R  QV++
Sbjct: 197 ALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIV 256

Query: 289 ASMKKMNLQLPSNGQGLYEGFGKE----GTGRMLHTEGFNFLDRAAILTPEEINLICNQG 348
           A+ +K+++++P +   L+E    E    G+ +++HT+   F D+AA+   E  +     G
Sbjct: 257 AAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV---ESQSDSIKDG 316

Query: 349 QTPNPWQICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFH 408
           +  NPW++C VTQVEE+K I+ LLPVW   I  + V+ QM ++FV QG  MD  +  NF 
Sbjct: 317 EV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFE 376

Query: 409 IPPASMTAFDIVSTTTFIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIA 468
           IP AS++ FD VS   +  +YD+ ++PL  + T RN +   +LQR+GIGL ++I A+I A
Sbjct: 377 IPSASLSLFDTVSVLFWTPVYDQFIIPLARKFT-RNERGFTQLQRMGIGLVVSIFAMITA 436

Query: 469 GFVEQKRLRHASVSG---KETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLK 528
           G +E  RL +        ++   +SIFWQ PQY+L+G +E F ++ Q++FF  Q P+ ++
Sbjct: 437 GVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMR 496

Query: 529 SLGMALSMSSTAMGSYLASLILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTAL 588
           SL  ALS+++ A+G+YL+++++T+VM ITK NGKPGW+P NLN GHLD FF+L A L+ L
Sbjct: 497 SLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFL 556

Query: 589 NLALYVYCAKRYK 595
           N  +Y++ +KRYK
Sbjct: 557 NFLVYLWISKRYK 561

BLAST of Lcy01g005480 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 2.1e-112
Identity = 229/595 (38.49%), Postives = 355/595 (59.66%), Query Frame = 0

Query: 12  SKGNGELQVADLHQLENGQERIQ------SSCSEEAALADQNEKTTAKGRKVGGWNCGML 71
           S    E ++ D+ +    QE +       SS +E+ ++       + K  K G W     
Sbjct: 4   SNEEDERRILDVEESLLTQEEVNNHLTGLSSTAEDGSIDIYGNPPSKK--KTGNWKACPF 63

Query: 72  LLVNQGFITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYL 131
           +L N+    LA+ G+  NL+ +  S L ++N  AA+    W GT Y   L+GA ++DSY 
Sbjct: 64  ILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYW 123

Query: 132 GRYLTCIIFQLVFIVGLIALSLLTHISLLKPNGC-GRIGELCDPHGLTELSIFYTSIYLI 191
           GRY T   F  ++ +G+  L+L   + +LKP  C G    LC P    + ++F+T +YLI
Sbjct: 124 GRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTVQYAVFFTGLYLI 183

Query: 192 ALGNGAPEPALATFGAEQFDENDPEENRAKTSFYSYFYVSINMGCLIAETVLVYIENLGH 251
           ALG G  +P +++FGA+QFD+ DP E   K SF+++FY SIN+G  I+ T+LV+++    
Sbjct: 184 ALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFISSTLLVWVQENVG 243

Query: 252 WMVGFWICAVSGLLAYLLLLSGTSRYRRSKPCGNPISRFSQVVMASMKKMNLQLPSNGQG 311
           W +GF I  V   ++      GT  YR  KP G+PI+R  QV++A+ +K+ L LP +   
Sbjct: 244 WGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYRKLKLNLPEDISF 303

Query: 312 LYEGFGKE----GTGRMLHTEGFNFLDRAAILTPEEINLICNQGQTPNPWQICPVTQVEE 371
           LYE   K     G+ ++ HT+G+ FLD+AA+++  E       G   NPW++C VTQVEE
Sbjct: 304 LYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYE----SKSGAFSNPWKLCTVTQVEE 363

Query: 372 VKCILRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMDTLVASNFHIPPASMTAFDIVSTTT 431
           VK ++R+ P+W   I  SV++ Q+ +LFV+QG +M+ ++ S F IPPAS   FD +    
Sbjct: 364 VKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRIIRS-FEIPPASFGVFDTLIVLI 423

Query: 432 FIMLYDRLLVPLYIRITKRNPKPPNELQRIGIGLAIAIVALIIAGFVEQKRLRHASVSGK 491
            I +YDR LVP   R T   PK   +LQR+GIGL ++++++  A  VE  RL+ A    +
Sbjct: 424 SIPIYDRFLVPFVRRFT-GIPKGLTDLQRMGIGLFLSVLSIAAAAIVETVRLQLA----Q 483

Query: 492 ETSSLSIFWQTPQYVLVGVSEAFVYVAQMDFFTSQIPEGLKSLGMALSMSSTAMGSYLAS 551
           +  ++SIFWQ PQY+L+G++E F ++ +++FF  + P+ ++S+  AL++ +TA+GSYL+S
Sbjct: 484 DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSS 543

Query: 552 LILTIVMAITKSNGKPGWVPPNLNDGHLDRFFFLSAALTALNLALY-VYCAKRYK 595
           LILT+V   T   GK GWVP +LN GHLD FF+L  +L  +N+ +Y + C K  K
Sbjct: 544 LILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTK 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQL26.8e-16152.77Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2[more]
Q8GXN24.9e-15152.57Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2[more]
Q3E9B58.4e-14347.25Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1[more]
Q9M3903.8e-11941.05Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q93Z202.9e-11138.49Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4180.0e+0088.94protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111460297 ... [more]
A0A6J1L1950.0e+0088.94protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111498952 PE... [more]
A0A6J1DSQ56.9e-31087.80protein NRT1/ PTR FAMILY 7.3-like isoform X5 OS=Momordica charantia OX=3673 GN=L... [more]
A0A1S3CD149.1e-31088.44protein NRT1/ PTR FAMILY 7.3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7T9M41.6e-30689.43Protein NRT1/ PTR FAMILY 7.3-like isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
XP_038875638.10.0e+0091.37protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Benincasa hispida][more]
XP_022959267.10.0e+0088.94protein NRT1/ PTR FAMILY 7.3-like [Cucurbita moschata] >KAG6574799.1 Protein NRT... [more]
XP_023006114.10.0e+0088.94protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima][more]
XP_023548509.14.7e-31088.29protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo][more]
XP_022155771.11.4e-30987.80protein NRT1/ PTR FAMILY 7.3-like isoform X5 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G32450.14.8e-16252.77nitrate transporter 1.5 [more]
AT4G21680.13.5e-15252.57NITRATE TRANSPORTER 1.8 [more]
AT5G19640.16.0e-14447.25Major facilitator superfamily protein [more]
AT3G54140.12.7e-12041.05peptide transporter 1 [more]
AT1G62200.12.1e-11238.49Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 129..559
e-value: 2.9E-82
score: 276.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 51..599
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 34..602
e-value: 2.6E-157
score: 526.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 58..604
NoneNo IPR availablePANTHERPTHR11654:SF384PROTEIN NRT1/ PTR FAMILY 7.3coord: 51..599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g005480.1Lcy01g005480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity