Lcy01g004830 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g004830
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr01: 6664547 .. 6666615 (-)
RNA-Seq ExpressionLcy01g004830
SyntenyLcy01g004830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATATATAAGAGAATGTAAAAAAGAAAAAGAAAAAGAACCACGAGAGAAAAAAGAAAGAAAAAGTTAGAAGATAAGGCAAGAGACTTGTCTGTTTCCCGAACCTCCGCCGCTACTTTTATTTTTTATTTTTCCTCTTCAAATATAAACTCTCTTAACTTTTCCTCCACCGCCGCCCCATTTTCCGGCCCCCCGCCGCCGCACGACTGAACCTCGATCACCTTCTCCGACGACTCAAGACTTCGAAGGATCTTCTTCCTCTGCAGATTCCGCTTCACATGGACTCCGTCGGAATTCCCGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAAGACTTTTGCACCGCCTTCTGTCCGCAAAACTGCCCCTTCATATTGCCGCCGCCGCCGCCGCTCTTCGGCCTCGACGACAGCGACGATTCCGGTACGTTCTTCTCGCCTCTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTCGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGGCGGAGAGTCGACGACGGCGGCGGAGGCGTCGGACCGGGAGAGAATTTCGAGACGGATCGAGCGATTAACGAAACGCGGCAGTCCGGTGCGGGACCTGGATTGGACGAGGCGCTGATTAAATCGATCTCGGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAAACGAGAGTTTGCGATTGTTGCCTAAATGTAGCCACGCTTTTCATCTCGCTTGCATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGCCGCTGCAATATCGCTTCCACGAATCCTCCGCCTCCGGAGAGTCCGCCGCCTTCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCCGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAGGCCGTCGTAGTCGAAATCGTAAACGATTCAACTAATCAAGATCAAGGAAGTCCGAATCCGAATCCAAATCCGCCGAGTTTCGTAACAGAGAGAAGAGAAGAATCCGTCCGTTCGATTAGGTCAGTTTCAGAGGATCATCAATCCTCTCCATGTCCAGGCCGCAATCTCTCCGTTGCAGACATATTGAGCATCAACCACGAAGAAGACGAAGAAATTGAAGCCGTGGATTCATCAACGGCGGCCGGAATCGGTCCCTCAAACGGCGGCGGCGGCAAAGAAACAGAGAAATCCAGAAACAGAAACGGCGTATTGAATTCCGCCATGAGAATGTCGATTTCAACTGGAATCTTCTCGTTCGCAAGGTATGCAAAAGGGAAGAACTCGAATTTACCAAATTGACTTCAAATTTTTTCAGAATCAAAAGTTTCGAAACAGAAAAAATCATTTGGCAAAGATCTTCAAAGAAATGTTGTTGAATCAAATTCTGTTTTGATCGACTGCAAAAACTGCAGTAAAATTTCTGGCGAATACAGAGTAGAGCTACGGTACTGGAGTATGTTGTTTCTGGAAGGCTCGAACAATTTTCCTTTTTTTTTTTTTCCTTATTTTTCCAAAGTCAAGATTGTCGGTTTGGACGGTTCTGATTTGGCCGTTCTGGTAATTTCCGGAAATTTATAGTTAATTTTAATGACAATCAAGTACTAAAGTTATAGGCTATTGTAATTAAGGCCCTCCAATTTTATGGATTTCAAAATTGGTCTCTCCAATTGTACCCATTGGATTGGATTGATGTTTTACCAATTTTGTTTGATTTCATAGTTTAAGTGGTCAACCATTTAGCTTACTGGCAACTAGGTACCTTTTTTTTCAAAATTTTTACCTCTCGATCGAAGTCATATGTTTGAACTAACTGAGTTATAGTCAAATATTTTGAGTTAGTTATTTTGTTGGAATGTACCTAAGATAAAAGCCAGTCAAGATACTTGTCAATTTTATCAAGATTTTTACTCAAAAGAAATTAGAATAACTATAATTTCATAAATAATTTTTATTTTATTTTATTTTTTTTAATAAAATGGGTGAAAATTTTGAATTTAT

mRNA sequence

AATATATATAAGAGAATGTAAAAAAGAAAAAGAAAAAGAACCACGAGAGAAAAAAGAAAGAAAAAGTTAGAAGATAAGGCAAGAGACTTGTCTGTTTCCCGAACCTCCGCCGCTACTTTTATTTTTTATTTTTCCTCTTCAAATATAAACTCTCTTAACTTTTCCTCCACCGCCGCCCCATTTTCCGGCCCCCCGCCGCCGCACGACTGAACCTCGATCACCTTCTCCGACGACTCAAGACTTCGAAGGATCTTCTTCCTCTGCAGATTCCGCTTCACATGGACTCCGTCGGAATTCCCGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAAGACTTTTGCACCGCCTTCTGTCCGCAAAACTGCCCCTTCATATTGCCGCCGCCGCCGCCGCTCTTCGGCCTCGACGACAGCGACGATTCCGGTACGTTCTTCTCGCCTCTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTCGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGGCGGAGAGTCGACGACGGCGGCGGAGGCGTCGGACCGGGAGAGAATTTCGAGACGGATCGAGCGATTAACGAAACGCGGCAGTCCGGTGCGGGACCTGGATTGGACGAGGCGCTGATTAAATCGATCTCGGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAAACGAGAGTTTGCGATTGTTGCCTAAATGTAGCCACGCTTTTCATCTCGCTTGCATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGCCGCTGCAATATCGCTTCCACGAATCCTCCGCCTCCGGAGAGTCCGCCGCCTTCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCCGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAGGCCGTCGTAGTCGAAATCGTAAACGATTCAACTAATCAAGATCAAGGAAGTCCGAATCCGAATCCAAATCCGCCGAGTTTCGTAACAGAGAGAAGAGAAGAATCCGTCCGTTCGATTAGGTCAGTTTCAGAGGATCATCAATCCTCTCCATGTCCAGGCCGCAATCTCTCCGTTGCAGACATATTGAGCATCAACCACGAAGAAGACGAAGAAATTGAAGCCGTGGATTCATCAACGGCGGCCGGAATCGGTCCCTCAAACGGCGGCGGCGGCAAAGAAACAGAGAAATCCAGAAACAGAAACGGCGTATTGAATTCCGCCATGAGAATGTCGATTTCAACTGGAATCTTCTCGTTCGCAAGGTATGCAAAAGGGAAGAACTCGAATTTACCAAATTGACTTCAAATTTTTTCAGAATCAAAAGTTTCGAAACAGAAAAAATCATTTGGCAAAGATCTTCAAAGAAATGTTGTTGAATCAAATTCTGTTTTGATCGACTGCAAAAACTGCAGTAAAATTTCTGGCGAATACAGAGTAGAGCTACGGTACTGGAGTATGTTGTTTCTGGAAGGCTCGAACAATTTTCCTTTTTTTTTTTTTCCTTATTTTTCCAAAGTCAAGATTGTCGGTTTGGACGGTTCTGATTTGGCCGTTCTGGTAATTTCCGGAAATTTATAGTTAATTTTAATGACAATCAAGTACTAAAGTTATAGGCTATTGTAATTAAGGCCCTCCAATTTTATGGATTTCAAAATTGGTCTCTCCAATTGTACCCATTGGATTGGATTGATGTTTTACCAATTTTGTTTGATTTCATAGTTTAAGTGGTCAACCATTTAGCTTACTGGCAACTAGGTACCTTTTTTTTCAAAATTTTTACCTCTCGATCGAAGTCATATGTTTGAACTAACTGAGTTATAGTCAAATATTTTGAGTTAGTTATTTTGTTGGAATGTACCTAAGATAAAAGCCAGTCAAGATACTTGTCAATTTTATCAAGATTTTTACTCAAAAGAAATTAGAATAACTATAATTTCATAAATAATTTTTATTTTATTTTATTTTTTTTAATAAAATGGGTGAAAATTTTGAATTTAT

Coding sequence (CDS)

ATGGACTCCGTCGGAATTCCCGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAAGACTTTTGCACCGCCTTCTGTCCGCAAAACTGCCCCTTCATATTGCCGCCGCCGCCGCCGCTCTTCGGCCTCGACGACAGCGACGATTCCGGTACGTTCTTCTCGCCTCTTATAGTCGCGGTGATCGGGATCTTGGCGAGCGCGTTCGTTCTCGTCACTTACTACGCGATCGTCTCGAAATATTGCCGGCGGAGAGTCGACGACGGCGGCGGAGGCGTCGGACCGGGAGAGAATTTCGAGACGGATCGAGCGATTAACGAAACGCGGCAGTCCGGTGCGGGACCTGGATTGGACGAGGCGCTGATTAAATCGATCTCGGTTTTCAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAAAACGAGAGTTTGCGATTGTTGCCTAAATGTAGCCACGCTTTTCATCTCGCTTGCATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGCCGCTGCAATATCGCTTCCACGAATCCTCCGCCTCCGGAGAGTCCGCCGCCTTCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCCGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAGGCCGTCGTAGTCGAAATCGTAAACGATTCAACTAATCAAGATCAAGGAAGTCCGAATCCGAATCCAAATCCGCCGAGTTTCGTAACAGAGAGAAGAGAAGAATCCGTCCGTTCGATTAGGTCAGTTTCAGAGGATCATCAATCCTCTCCATGTCCAGGCCGCAATCTCTCCGTTGCAGACATATTGAGCATCAACCACGAAGAAGACGAAGAAATTGAAGCCGTGGATTCATCAACGGCGGCCGGAATCGGTCCCTCAAACGGCGGCGGCGGCAAAGAAACAGAGAAATCCAGAAACAGAAACGGCGTATTGAATTCCGCCATGAGAATGTCGATTTCAACTGGAATCTTCTCGTTCGCAAGGTATGCAAAAGGGAAGAACTCGAATTTACCAAATTGA

Protein sequence

MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVNDSTNQDQGSPNPNPNPPSFVTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAKGKNSNLPN
Homology
BLAST of Lcy01g004830 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.4e-58
Identity = 151/375 (40.27%), Postives = 208/375 (55.47%), Query Frame = 0

Query: 7   PDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGL-DDSDDSGTFFSPLIVAVI 66
           P+P  P++ +N  DCSQ  C  +CPQ C  I PPPPP F L DDS  S + FSPL++A+I
Sbjct: 5   PNPWSPYDSYN--DCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALI 64

Query: 67  GILASAFVLVTYYAIVSKYCRRRVDDGGGGV----GPGENFETDRAINETRQSGAGPGLD 126
           GIL SA +LV+YY ++SKYC R               GE F +    +  R S  G GL+
Sbjct: 65  GILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFS----STQRISTNGDGLN 124

Query: 127 EALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 186
           E++IKSI+V+K+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHL CIDTWLKS+S
Sbjct: 125 ESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHS 184

Query: 187 SCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVS---------QEA 246
           +CPLCR  +   N P           +  + +S      V +++ N++            
Sbjct: 185 NCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSV 244

Query: 247 VVVEIVN-DSTNQDQGSPNPNPNPPSFVTERR----EESVRSIRSVSEDHQSSPCPGRNL 306
           VV+E +   S N D  S    P       ERR    E+S+   RSVS +       G  +
Sbjct: 245 VVIEDLEIGSRNSDARSELQLPE------ERRETKDEDSLPIRRSVSLN------SGVVV 304

Query: 307 SVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGI 363
           S+AD+L    +E+ E   V +S     G    G      ++  R+G ++     S+STG 
Sbjct: 305 SIADVLREIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGR 361

BLAST of Lcy01g004830 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 1.3e-55
Identity = 147/381 (38.58%), Postives = 206/381 (54.07%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTF-FSP 60
           M S G P+P       +++DCSQ  C+ +CPQ C +++ PPPP F LDD DDS +  FSP
Sbjct: 1   MGSTGNPNPWGTTYD-SYRDCSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSDFSP 60

Query: 61  LIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGG------------VGPGENFETDRA 120
           L++A+IGILASAF+LV+YY ++SKYC RR  +                   G N   +  
Sbjct: 61  LLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNG 120

Query: 121 I---NETRQSGAGPGLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKC 180
               N+T   G G GLDE+LIKSI+V+K++K +G +E SDCSVCLSEFQENESLRLLPKC
Sbjct: 121 ATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKC 180

Query: 181 SHAFHLACIDTWLKSNSSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDL 240
           +HAFH+ CIDTWLKS+S+CPLCR  I +++         +V  +   +      +V ++ 
Sbjct: 181 NHAFHVPCIDTWLKSHSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENN 240

Query: 241 SANVSQEAVVV---EIVNDSTNQDQGSPNPNPNPPSFVTERREESVRSIRSVSEDHQSSP 300
           S +   ++VVV   ++ N  +  +  +    P PP     R  E  RS         S  
Sbjct: 241 SISTGDDSVVVVNLDLENSRSRNETVNEGSTPKPPEMQDSRDGEERRS--------ASLN 300

Query: 301 CPGRNLSVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRM 360
             G  +S+ADIL    E+DEE   V +S     G            +   NG+ N  +R 
Sbjct: 301 SGGGVVSIADILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRS 355

Query: 361 SISTGIFSFARYAKGKNSNLP 363
           S++      + Y + KN  LP
Sbjct: 361 SMAA--MKRSGYDRAKNYRLP 355

BLAST of Lcy01g004830 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 1.8e-47
Identity = 137/359 (38.16%), Postives = 185/359 (51.53%), Query Frame = 0

Query: 12  PFNPFNFKDCSQDFCTAFCPQNCPFILPPPPP--LFGLDDSDDSGTFFSPLIVAVIGILA 71
           P+ P   +DCSQ  C+ +CPQ C FI PPPPP  + G    D SG  FSPL++A+IG+LA
Sbjct: 10  PYEP--TRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 69

Query: 72  SAFVLVTYYAIVSKYC------RRRVDDG-------GGGVGPGENFETDRAINETRQSGA 131
           SAF+LV+YY  +SKYC      R RV          GGG G G      ++ +    + +
Sbjct: 70  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVS 129

Query: 132 GP-GLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDT 191
            P GLDE LI  I+V K+++G+G +  +DCSVCL EF + ESLRLLP+CSHAFH  CIDT
Sbjct: 130 PPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDT 189

Query: 192 WLKSNSSCPLCRCNIASTN----PPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQE 251
           WLKS+S+CPLCR NI         P PE   P  T     H     VVVV D   N+ +E
Sbjct: 190 WLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHE----VVVVMDGLENLCEE 249

Query: 252 -------AVVVEIVNDSTNQDQGSPNPNPNPPSFVTERREESVRSIRSVS--EDHQSSPC 311
                  A   +  +D+ +  +G    N        E REE     R  S  + H+ +  
Sbjct: 250 QQEAVSRASTADDDHDAKDVAEGMEEANG-----AAEIREEGSPPKRGASSFDLHRDN-- 309

Query: 312 PGRNLSVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRM 342
               + +AD+L  + E++          A G G S    G   E S+ R G    A+++
Sbjct: 310 ---RMCIADVLQESMEDELTAARESGLLAGGAGTSRRCHG---ENSKGRGGRSRRALQL 349

BLAST of Lcy01g004830 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 88/207 (42.51%), Postives = 115/207 (55.56%), Query Frame = 0

Query: 7   PDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLF---GLDDSDDSGTFFSPLIVA 66
           P+PS      N +DC+Q  C+ FCPQ C +I   PPP+     L+D   S    SPL++A
Sbjct: 5   PNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLVIA 64

Query: 67  VIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQ---------S 126
           + GI A+AF+L  YY +VSKYC     +         +   D  +N   +          
Sbjct: 65  IFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILD--VNSPERGDQDDPFALE 124

Query: 127 GAGPGLDEALIKSISVFKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSHAFHLAC 186
            +  GLD+ LIK I  FK KK +    I G+DCS+CL EF E+ESLRLLPKC+H FH+ C
Sbjct: 125 SSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVC 184

Query: 187 IDTWLKSNSSCPLCRCNIASTNPPPPE 200
           ID WLKS+S+CPLCR  I       PE
Sbjct: 185 IDRWLKSHSNCPLCRAKIIVPTTQQPE 209

BLAST of Lcy01g004830 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.0e-27
Identity = 89/228 (39.04%), Postives = 122/228 (53.51%), Query Frame = 0

Query: 39  PPPPPLFGLDDSDDSGTFFSPLIVAVIGILASAFVLVTYYAIVSKYCR--RRVDDGGGGV 98
           PPPPP+F    +  +GT F  L VAVIGILA+AF+LV+YY  V K C    R+D  G   
Sbjct: 18  PPPPPIF--HRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFS 77

Query: 99  GPGENFETDRAINETRQSGAGPGLDEALIKSISVFKFKK----------GEGLIE----G 158
                      +          GLDE++I++I +FKFKK          GEG  E     
Sbjct: 78  LSRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRS 137

Query: 159 SDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPLCRCNIA-STNPPPPESP 218
            +CSVCLSEFQ+ E LR++P CSH FH+ CID WL++N++CPLCR  ++  T+ PP    
Sbjct: 138 QECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVS 197

Query: 219 PPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVNDSTNQDQGSP 250
            PS        +S   +V+++        E VV+E+   S   D+ SP
Sbjct: 198 APS--------TSPENLVMLRG-----ENEYVVIEL-GSSIGSDRDSP 229

BLAST of Lcy01g004830 vs. ExPASy TrEMBL
Match: A0A6J1HMW7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.3e-167
Identity = 310/368 (84.24%), Postives = 328/368 (89.13%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           M+S+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q +PNP+PNP SF      TERREESV SIRSVSEDH SSPCPG NLSVAD+L+I
Sbjct: 241 STNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 360

Query: 361 GKNSNLPN 364
           GKNSN PN
Sbjct: 361 GKNSNSPN 362

BLAST of Lcy01g004830 vs. ExPASy TrEMBL
Match: A0A6J1KJE3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.4e-164
Identity = 308/368 (83.70%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           MDS+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q     NPNP SF      TERREES+ SIRSVSEDH SSPCPG NLSVAD+LSI
Sbjct: 241 STNQEQ----ENPNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 358

Query: 361 GKNSNLPN 364
           GKN N PN
Sbjct: 361 GKNPNSPN 358

BLAST of Lcy01g004830 vs. ExPASy TrEMBL
Match: A0A6J1DSH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022765 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.9e-162
Identity = 311/376 (82.71%), Postives = 328/376 (87.23%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           MDSVGIPDPSPPFNPFN KDCSQ  CTAFCPQ C ++ PPPPP FGLDDSDDSGTFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNLKDCSQGICTAFCPQWCRYVFPPPPPFFGLDDSDDSGTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGV--GPGENFETDRAINETRQ-SGAG 120
           IVAVIGILASAFVLVTYYAIVSKYCRRRVD GGGG   GPG+ FETDRA+N+ R+ SGAG
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDSGGGGAGGGPGDYFETDRAMNDARRPSGAG 120

Query: 121 PGLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWL 180
           PGLDEALIKSISVFKFKK EGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWL
Sbjct: 121 PGLDEALIKSISVFKFKKNEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLVCIDTWL 180

Query: 181 KSNSSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEI 240
           KSNSSCPLCRCNIASTNP PPE+PPPSVTALGYRH SDAAVVVVQD S NVSQEAVVVEI
Sbjct: 181 KSNSSCPLCRCNIASTNPNPPETPPPSVTALGYRHRSDAAVVVVQDPSENVSQEAVVVEI 240

Query: 241 VNDS----TNQDQGSPNPNPNPPSFV----TERREESVRSIRSVSEDHQS--SPCPGRNL 300
           +  S    +NQDQG  N NP P S      TERREE+VRSIRSVSED QS  SPC GRNL
Sbjct: 241 IGHSISNPSNQDQGGSNSNP-PSSEQGDGGTERREETVRSIRSVSEDQQSSDSPCLGRNL 300

Query: 301 SVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGI 360
           SVADILSI+H+EDEE+EAVDSS AAGIGPSN   GKET KSR RNGVLNSAMRMSI +GI
Sbjct: 301 SVADILSISHDEDEEVEAVDSSMAAGIGPSN-DDGKETGKSRIRNGVLNSAMRMSIPSGI 360

Query: 361 FSFARYAKGKNSNLPN 364
           FSFA++AKGK+SNLPN
Sbjct: 361 FSFAKHAKGKSSNLPN 374

BLAST of Lcy01g004830 vs. ExPASy TrEMBL
Match: A0A6J1H2X8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.4e-162
Identity = 309/371 (83.29%), Postives = 323/371 (87.06%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNC--PFILPPPPPLFGLDDSDDSGTFFS 60
           MDSVGIPDPSPPFNPFNF DCSQ+ CTAFCPQ C  PF   PPPPLFGLDDSDDS TFFS
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPF---PPPPLFGLDDSDDSDTFFS 60

Query: 61  PLIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGP 120
           PLIVAVIGILASAFVLVTYYAIVSKYCRRRVDD GGG  P ENFE DR +N+TRQS AGP
Sbjct: 61  PLIVAVIGILASAFVLVTYYAIVSKYCRRRVDD-GGGAEPVENFEADRVVNDTRQSSAGP 120

Query: 121 GLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLK 180
           GL+EAL+KSISVFKFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHLACIDTWLK
Sbjct: 121 GLNEALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLK 180

Query: 181 SNSSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIV 240
           SNSSCP CR NI  TNPPPPE+PPPSVT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV
Sbjct: 181 SNSSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIV 240

Query: 241 NDSTNQDQGSPNPNPNP-PSFV------TER-REESVRSIRSVSEDHQSSPCPGRNLSVA 300
            DSTN+DQGSPNPNPNP P  V      TER REES+RSIRSVSEDHQSSPCP R LSVA
Sbjct: 241 TDSTNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVA 300

Query: 301 DILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSF 360
           DILSIN EEDEE+EA++SS AAG GPSN   GKETEKSRNRN VLNSAMRMSI +GIFSF
Sbjct: 301 DILSINEEEDEEVEAMNSSMAAGSGPSN-NDGKETEKSRNRNSVLNSAMRMSIPSGIFSF 360

Query: 361 ARYAKGKNSNL 362
            RY KGK SNL
Sbjct: 361 QRYVKGKCSNL 366

BLAST of Lcy01g004830 vs. ExPASy TrEMBL
Match: A0A6J1L482 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.2e-160
Identity = 301/367 (82.02%), Postives = 316/367 (86.10%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           MDSVGIPDPSPPFNPFNF DCSQ  CTAFCPQ C F   PPPPLFGLDDSD S TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQKLCTAFCPQWCAFPF-PPPPLFGLDDSDKSDTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRRRVDD GGG  P ENFE DR  N+TRQS AGPGL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDD-GGGAEPVENFEADRVANDTRQSSAGPGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           +EALIKSISVFKFKKGEGL+E SDC++CLSEFQENESLRLLPKCSHAFHLACIDTWLKSN
Sbjct: 121 NEALIKSISVFKFKKGEGLVEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCP CR NI  TNPPPPE+PPPSVT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV D
Sbjct: 181 SSCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTD 240

Query: 241 STNQDQGSPNPNPNPPSF------VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILS 300
           STN+DQGSPNPNPNPPS         + REES+RSIRSVSEDHQSSPCP R LSVADILS
Sbjct: 241 STNRDQGSPNPNPNPPSVDRGDGRTEKNREESIRSIRSVSEDHQSSPCPVRILSVADILS 300

Query: 301 INHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYA 360
           I+ EEDEE+EA++S  AA  GPSN   GKETEKSRNRN VLNSAMRMSI +G+FSF RYA
Sbjct: 301 IDEEEDEEVEAMNSPMAADTGPSN-NDGKETEKSRNRNSVLNSAMRMSIPSGVFSFQRYA 360

Query: 361 KGKNSNL 362
           KGK SNL
Sbjct: 361 KGKCSNL 364

BLAST of Lcy01g004830 vs. NCBI nr
Match: XP_022964349.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata])

HSP 1 Score: 598.2 bits (1541), Expect = 4.7e-167
Identity = 310/368 (84.24%), Postives = 328/368 (89.13%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           M+S+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q +PNP+PNP SF      TERREESV SIRSVSEDH SSPCPG NLSVAD+L+I
Sbjct: 241 STNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 360

Query: 361 GKNSNLPN 364
           GKNSN PN
Sbjct: 361 GKNSNSPN 362

BLAST of Lcy01g004830 vs. NCBI nr
Match: KAG7026103.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.4 bits (1539), Expect = 8.1e-167
Identity = 310/368 (84.24%), Postives = 328/368 (89.13%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           M+S+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q +PNP+PNP SF      TERREESV SIRSVSEDH SSPCPG NLSVAD+L+I
Sbjct: 241 STNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 360

Query: 361 GKNSNLPN 364
           GKNSN PN
Sbjct: 361 GKNSNSPN 362

BLAST of Lcy01g004830 vs. NCBI nr
Match: KAG6593770.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-166
Identity = 309/368 (83.97%), Postives = 328/368 (89.13%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           M+S+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYC+R V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCQRSVGDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q +PNP+PNP SF      TERREESV SIRSVSEDH SSPCPG NLSVAD+L+I
Sbjct: 241 STNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 360

Query: 361 GKNSNLPN 364
           GKNSN PN
Sbjct: 361 GKNSNSPN 362

BLAST of Lcy01g004830 vs. NCBI nr
Match: XP_023000254.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 588.2 bits (1515), Expect = 4.9e-164
Identity = 308/368 (83.70%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           MDS+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSDAAVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q     NPNP SF      TERREES+ SIRSVSEDH SSPCPG NLSVAD+LSI
Sbjct: 241 STNQEQ----ENPNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN G  KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-GNNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 358

Query: 361 GKNSNLPN 364
           GKN N PN
Sbjct: 361 GKNPNSPN 358

BLAST of Lcy01g004830 vs. NCBI nr
Match: XP_023513580.1 (RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.4 bits (1500), Expect = 2.7e-162
Identity = 304/368 (82.61%), Postives = 322/368 (87.50%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTFFSPL 60
           MDS+GI DPSPPFNPFNFKDCSQ  CTAFCPQ C F+ PPPPP FGLDDSDDS TFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSGAGPGL 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGGG GP E+FET+RAINETR+S AG GL
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGG-GPEESFETNRAINETRRSSAGSGL 120

Query: 121 DEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSN 180
           DEALIKSISV+KFKKGEGLIEGSDCSVCLSEFQENE+LRLLPKCSHAFHL CIDTWLKSN
Sbjct: 121 DEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENENLRLLPKCSHAFHLPCIDTWLKSN 180

Query: 181 SSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVND 240
           SSCPLCR NIASTNPPP E+PPP VTALGYRHSSD AVVVVQDLSANVSQE+VVVEIV+D
Sbjct: 181 SSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDTAVVVVQDLSANVSQESVVVEIVSD 240

Query: 241 STNQDQGSPNPNPNPPSF-----VTERREESVRSIRSVSEDHQSSPCPGRNLSVADILSI 300
           STNQ+Q     NPNP SF      TERREES+ SIRSVSEDH S+PCPG NLSVAD+L+I
Sbjct: 241 STNQEQ----ENPNPRSFDQGHGRTERREESIHSIRSVSEDHHSAPCPGENLSVADVLNI 300

Query: 301 NHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGIFSFARYAK 360
           NHEEDEEIEA+DSS AAGIGPSN    KETEKSRN+NGVL    RMSIS+GIFSF RY K
Sbjct: 301 NHEEDEEIEALDSSMAAGIGPSN-ANNKETEKSRNKNGVL----RMSISSGIFSFTRYTK 358

Query: 361 GKNSNLPN 364
           GKNSN PN
Sbjct: 361 GKNSNSPN 358

BLAST of Lcy01g004830 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 4.6e-59
Identity = 151/375 (40.27%), Postives = 208/375 (55.47%), Query Frame = 0

Query: 7   PDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGL-DDSDDSGTFFSPLIVAVI 66
           P+P  P++ +N  DCSQ  C  +CPQ C  I PPPPP F L DDS  S + FSPL++A+I
Sbjct: 5   PNPWSPYDSYN--DCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALI 64

Query: 67  GILASAFVLVTYYAIVSKYCRRRVDDGGGGV----GPGENFETDRAINETRQSGAGPGLD 126
           GIL SA +LV+YY ++SKYC R               GE F +    +  R S  G GL+
Sbjct: 65  GILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFS----STQRISTNGDGLN 124

Query: 127 EALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 186
           E++IKSI+V+K+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHL CIDTWLKS+S
Sbjct: 125 ESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHS 184

Query: 187 SCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDLSANVS---------QEA 246
           +CPLCR  +   N P           +  + +S      V +++ N++            
Sbjct: 185 NCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSV 244

Query: 247 VVVEIVN-DSTNQDQGSPNPNPNPPSFVTERR----EESVRSIRSVSEDHQSSPCPGRNL 306
           VV+E +   S N D  S    P       ERR    E+S+   RSVS +       G  +
Sbjct: 245 VVIEDLEIGSRNSDARSELQLPE------ERRETKDEDSLPIRRSVSLN------SGVVV 304

Query: 307 SVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRMSISTGI 363
           S+AD+L    +E+ E   V +S     G    G      ++  R+G ++     S+STG 
Sbjct: 305 SIADVLREIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGR 361

BLAST of Lcy01g004830 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 9.5e-57
Identity = 147/381 (38.58%), Postives = 206/381 (54.07%), Query Frame = 0

Query: 1   MDSVGIPDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLFGLDDSDDSGTF-FSP 60
           M S G P+P       +++DCSQ  C+ +CPQ C +++ PPPP F LDD DDS +  FSP
Sbjct: 1   MGSTGNPNPWGTTYD-SYRDCSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSDFSP 60

Query: 61  LIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGG------------VGPGENFETDRA 120
           L++A+IGILASAF+LV+YY ++SKYC RR  +                   G N   +  
Sbjct: 61  LLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNG 120

Query: 121 I---NETRQSGAGPGLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKC 180
               N+T   G G GLDE+LIKSI+V+K++K +G +E SDCSVCLSEFQENESLRLLPKC
Sbjct: 121 ATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKC 180

Query: 181 SHAFHLACIDTWLKSNSSCPLCRCNIASTNPPPPESPPPSVTALGYRHSSDAAVVVVQDL 240
           +HAFH+ CIDTWLKS+S+CPLCR  I +++         +V  +   +      +V ++ 
Sbjct: 181 NHAFHVPCIDTWLKSHSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENN 240

Query: 241 SANVSQEAVVV---EIVNDSTNQDQGSPNPNPNPPSFVTERREESVRSIRSVSEDHQSSP 300
           S +   ++VVV   ++ N  +  +  +    P PP     R  E  RS         S  
Sbjct: 241 SISTGDDSVVVVNLDLENSRSRNETVNEGSTPKPPEMQDSRDGEERRS--------ASLN 300

Query: 301 CPGRNLSVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRNGVLNSAMRM 360
             G  +S+ADIL    E+DEE   V +S     G            +   NG+ N  +R 
Sbjct: 301 SGGGVVSIADILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRS 355

Query: 361 SISTGIFSFARYAKGKNSNLP 363
           S++      + Y + KN  LP
Sbjct: 361 SMAA--MKRSGYDRAKNYRLP 355

BLAST of Lcy01g004830 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 8.9e-39
Identity = 88/207 (42.51%), Postives = 115/207 (55.56%), Query Frame = 0

Query: 7   PDPSPPFNPFNFKDCSQDFCTAFCPQNCPFILPPPPPLF---GLDDSDDSGTFFSPLIVA 66
           P+PS      N +DC+Q  C+ FCPQ C +I   PPP+     L+D   S    SPL++A
Sbjct: 5   PNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLVIA 64

Query: 67  VIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQ---------S 126
           + GI A+AF+L  YY +VSKYC     +         +   D  +N   +          
Sbjct: 65  IFGIFATAFLLAAYYTLVSKYCANDTTNEAASESGRSDIILD--VNSPERGDQDDPFALE 124

Query: 127 GAGPGLDEALIKSISVFKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSHAFHLAC 186
            +  GLD+ LIK I  FK KK +    I G+DCS+CL EF E+ESLRLLPKC+H FH+ C
Sbjct: 125 SSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFHVVC 184

Query: 187 IDTWLKSNSSCPLCRCNIASTNPPPPE 200
           ID WLKS+S+CPLCR  I       PE
Sbjct: 185 IDRWLKSHSNCPLCRAKIIVPTTQQPE 209

BLAST of Lcy01g004830 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-28
Identity = 89/228 (39.04%), Postives = 122/228 (53.51%), Query Frame = 0

Query: 39  PPPPPLFGLDDSDDSGTFFSPLIVAVIGILASAFVLVTYYAIVSKYCR--RRVDDGGGGV 98
           PPPPP+F    +  +GT F  L VAVIGILA+AF+LV+YY  V K C    R+D  G   
Sbjct: 18  PPPPPIF--HRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFS 77

Query: 99  GPGENFETDRAINETRQSGAGPGLDEALIKSISVFKFKK----------GEGLIE----G 158
                      +          GLDE++I++I +FKFKK          GEG  E     
Sbjct: 78  LSRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRS 137

Query: 159 SDCSVCLSEFQENESLRLLPKCSHAFHLACIDTWLKSNSSCPLCRCNIA-STNPPPPESP 218
            +CSVCLSEFQ+ E LR++P CSH FH+ CID WL++N++CPLCR  ++  T+ PP    
Sbjct: 138 QECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVS 197

Query: 219 PPSVTALGYRHSSDAAVVVVQDLSANVSQEAVVVEIVNDSTNQDQGSP 250
            PS        +S   +V+++        E VV+E+   S   D+ SP
Sbjct: 198 APS--------TSPENLVMLRG-----ENEYVVIEL-GSSIGSDRDSP 229

BLAST of Lcy01g004830 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 3.9e-26
Identity = 105/364 (28.85%), Postives = 161/364 (44.23%), Query Frame = 0

Query: 20  DCSQDFCTAFCPQNC-------------------PFILPPPPPLFG---LDDSDDSGTFF 79
           DCS   C   CP NC                   P   P P P      L   D S +  
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 80  SPLIVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGGVGPGENFETDRAINETRQSG-- 139
           +  I+ + G + +  +L+T + +V+K+    V+    G    +N + D  + E  Q    
Sbjct: 82  AISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ 141

Query: 140 --------AGPGLDEALIKSISVFKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHA 199
                      GL +++I SI++  +K+G+GLIE +DC VCL+EF+E+ESLRLLPKC+HA
Sbjct: 142 VDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHA 201

Query: 200 FHLACIDTWLKSNSSCPLCRCNIASTNPPPPE-SPPPSVTALGYRHSSDAAVVVVQD--- 259
           FH++CIDTWL S+++CPLCR  IA  +   P  S P  VT  G     +   V  +D   
Sbjct: 202 FHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGSHLENDGVDEEDHGE 261

Query: 260 LSANVSQE-------AVVVEIVNDSTNQDQGSPNPNPNPPSFVTERRE----ESVRSIRS 319
           +   V  +        + +EI    ++ D GS          + E  +    +SV S+  
Sbjct: 262 IENRVDSDFKESDDSDIRIEIYRFDSDGD-GSETETKERVRVLKECMDPNGGDSVNSLSH 321

Query: 320 VSEDHQSSPCPGRNLSVADILSINHEEDEEIEAVDSSTAAGIGPSNGGGGKETEKSRNRN 337
                +S   PG++            + EE    +    A     NGGG  +  +S + +
Sbjct: 322 TKTHVESVDFPGKSC---------ENQSEEFTRHNGEDEASCSEENGGGSNQLRRSCD-S 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF646.4e-5840.27RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ81.3e-5538.58RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY81.8e-4738.16E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0411.3e-3742.51Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q9LSW92.0e-2739.04RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HMW72.3e-16784.24RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384... [more]
A0A6J1KJE32.4e-16483.70RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 P... [more]
A0A6J1DSH14.9e-16282.71RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102276... [more]
A0A6J1H2X88.4e-16283.29RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995... [more]
A0A6J1L4821.2e-16082.02RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 P... [more]
Match NameE-valueIdentityDescription
XP_022964349.14.7e-16784.24RING-H2 finger protein ATL52-like [Cucurbita moschata][more]
KAG7026103.18.1e-16784.24RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6593770.11.4e-16683.97RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023000254.14.9e-16483.70RING-H2 finger protein ATL52-like [Cucurbita maxima][more]
XP_023513580.12.7e-16282.61RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G17600.14.6e-5940.27RING/U-box superfamily protein [more]
AT3G03550.19.5e-5738.58RING/U-box superfamily protein [more]
AT4G17905.18.9e-3942.51RING/U-box superfamily protein [more]
AT5G43420.11.4e-2839.04RING/U-box superfamily protein [more]
AT1G72220.13.9e-2628.85RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 145..186
e-value: 3.8E-8
score: 43.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 144..187
e-value: 7.7E-14
score: 51.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 145..187
score: 13.153163
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 120..194
e-value: 4.7E-22
score: 79.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..337
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 240..287
NoneNo IPR availablePANTHERPTHR45676:SF108E3 UBIQUITIN-PROTEIN LIGASE OS04G0590900-LIKEcoord: 16..224
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 144..187
e-value: 6.85826E-18
score: 74.2605
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 139..193
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 16..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g004830.1Lcy01g004830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane