Lcy01g004470 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g004470
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionVacuolar protein sorting-associated protein 35
LocationChr01: 5993130 .. 5994123 (+)
RNA-Seq ExpressionLcy01g004470
SyntenyLcy01g004470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGTGTCTCAGATTTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAGATATGGCAGAGTATTGCCCTCTCTCTATCTCTCTTTCTTTTTCCCAATCTCTCTCTCCTATCTCTCTTTTTCCCCTTTTATGATTTTGGGTTTTGTTCTTTGTTGCAGTACAATGAAATTTTGGGAAAAGGAGCTTCAAAAACAGTGTAGGGATTTGATTTTCTTGCATCTTATGGGCTTCCCATTTTTCCATTTTCTAGAAATTTGCAAATTCTTCTGAAGCATTTTAATGGGTTTTTGTTTGTTAATTTATTACAATTTATGAAAATGAGTAGAAAATGGAGTTCAATGACTATACATTGCGAACCTTTCAAATGGAAATGCTTGTATGTATGTCCAGTCCAACTCTTTACATATACTCAATTTGAAAAATTTTATTTTACGTCCTTTTAATGATTACATTATTTCTGTCTGAAAGATGGCATGTGAACAACTTTACGACTATGAATAATTTTGGTCCAAGACCCTGACAAAGCTACCTCATGGTGCAGTACTCTGCAAAGCTTTTAAAAAAGCTTGATCAATGTCGAGCTGTTTATGCTTGCTTGCATCTCTTCTGGCTAGATGATCATGATAATATGAAGGATGGTGAGAGGTGAGTTACAAACTTACAAGTACGTTTATTACAGTGGCTATGGAGCTGAGCAGGGTGAAAAGGTACACAATTCAGTTCCTGACAAACTTTGTTACTCCTGCTGGCTTTTTTAAGCGAATTTGTGCATTTGACATCTTACACTTAAGCTTCTATATTTGGGAGAATTGAAACAAATAAAAGTTTATTGACATTGGGGCGAGTGATCAATGCACTAGTGGAGGATCTTGGGCATATCCCATATAGGTTAGCGGCTAGTGTTAGTGCTTTGACAAATTCTTATGATGTTTCATTCCACATTATAATTATTGCTTACGTGATTGAACTTCGGGGATAG

mRNA sequence

ATGGATGGTGTCTCAGATTTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAGATATGGCAGATACTCTGCAAAGCTTTTAAAAAAGCTTGATCAATGTCGAGCTGTTTATGCTTGCTTGCATCTCTTCTGGCTAGATGATCATGATAATATGAAGGATGGTGAGAGGTTAGCGGCTAGTGTTAGTGCTTTGACAAATTCTTATGATGTTTCATTCCACATTATAATTATTGCTTACGTGATTGAACTTCGGGGATAG

Coding sequence (CDS)

ATGGATGGTGTCTCAGATTTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAGATATGGCAGATACTCTGCAAAGCTTTTAAAAAAGCTTGATCAATGTCGAGCTGTTTATGCTTGCTTGCATCTCTTCTGGCTAGATGATCATGATAATATGAAGGATGGTGAGAGGTTAGCGGCTAGTGTTAGTGCTTTGACAAATTCTTATGATGTTTCATTCCACATTATAATTATTGCTTACGTGATTGAACTTCGGGGATAG

Protein sequence

MDGVSDFEPDCSEFVEVDPTGRYGRYSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERLAASVSALTNSYDVSFHIIIIAYVIELRG
Homology
[Close]
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match: F4I0P8 (Vacuolar protein sorting-associated protein 35B OS=Arabidopsis thaliana OX=3702 GN=VPS35B PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.2e-10
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSA+LLKK DQCRAVYAC HLFW+DD D +KDGER+
Sbjct: 649 YSARLLKKPDQCRAVYACSHLFWVDDPDGIKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match: Q7X659 (Vacuolar protein sorting-associated protein 35A OS=Arabidopsis thaliana OX=3702 GN=VPS35A PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.9e-10
Identity = 26/36 (72.22%), Postives = 32/36 (88.89%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           Y+AKLLKK DQCRAVYAC HLFWL+D + ++DGER+
Sbjct: 649 YAAKLLKKPDQCRAVYACSHLFWLEDRETIQDGERV 684

[Close]
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match: A8R7K9 (Vacuolar protein sorting-associated protein 35C OS=Arabidopsis thaliana OX=3702 GN=VPS35C PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.3e-09
Identity = 25/36 (69.44%), Postives = 31/36 (86.11%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSA+LL+K DQCRAVY C HLFW D+ +N+KDGER+
Sbjct: 646 YSARLLRKPDQCRAVYECAHLFWADECENLKDGERV 681

[Close]
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 7.7e-05
Identity = 22/32 (68.75%), Postives = 25/32 (78.12%), Query Frame = 0 

Query: 1  MDGVSDFEPDCSEFVEVDPTGRYGRYSAKLLK 33
          M+ +S  EPD SEFVEVDPTGRYGRY+  L K
Sbjct: 1  MNNLSYLEPDYSEFVEVDPTGRYGRYNEVLGK 32

[Close]
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match: P34110 (Vacuolar protein sorting-associated protein 35 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS35 PE=1 SV=2)

HSP 1 Score: 45.1 bits (105), Expect = 5.0e-04
Identity = 18/34 (52.94%), Postives = 24/34 (70.59%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGE 60
           + +KLLKK DQCRAVY C HL+W  +  N+ + E
Sbjct: 723 HGSKLLKKQDQCRAVYLCSHLWWATEISNIGEEE 756

[Close]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match: A0A4P1RIC9 (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_22864 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.7e-12
Identity = 33/38 (86.84%), Postives = 36/38 (94.74%), Query Frame = 0 

Query: 24  GRYSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           G+YSAKLLKK DQCRAVYAC HLFW+DDHDNMKDGER+
Sbjct: 146 GQYSAKLLKKRDQCRAVYACSHLFWVDDHDNMKDGERI 183

[Close]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match: A0A6J1L3G7 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita maxima OX=3661 GN=LOC111500194 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.3e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 649 YSAKLLKKSDQCRAVYACSHLFWLDDHDNMKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match: A0A6J1E644 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita moschata OX=3662 GN=LOC111430951 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.3e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 649 YSAKLLKKSDQCRAVYACSHLFWLDDHDNMKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match: A0A5A7UCN0 (Vacuolar protein sorting-associated protein 35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00570 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.4e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 649 YSAKLLKKPDQCRAVYACSHLFWLDDHDNMKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match: A0A0A0L666 (Vacuolar protein sorting-associated protein 35 OS=Cucumis sativus OX=3659 GN=Csa_3G002660 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.4e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 635 YSAKLLKKPDQCRAVYACSHLFWLDDHDNMKDGERV 670

[Close]
BLAST of Lcy01g004470 vs. NCBI nr
Match: OIW11057.1 (hypothetical protein TanjilG_22864 [Lupinus angustifolius])

HSP 1 Score: 79.7 bits (195), Expect = 1.4e-11
Identity = 33/38 (86.84%), Postives = 36/38 (94.74%), Query Frame = 0 

Query: 24  GRYSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           G+YSAKLLKK DQCRAVYAC HLFW+DDHDNMKDGER+
Sbjct: 146 GQYSAKLLKKRDQCRAVYACSHLFWVDDHDNMKDGERI 183

[Close]
BLAST of Lcy01g004470 vs. NCBI nr
Match: XP_019444852.1 (PREDICTED: aconitate hydratase-like isoform X1 [Lupinus angustifolius])

HSP 1 Score: 79.7 bits (195), Expect = 1.4e-11
Identity = 33/38 (86.84%), Postives = 36/38 (94.74%), Query Frame = 0 

Query: 24 GRYSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
          G+YSAKLLKK DQCRAVYAC HLFW+DDHDNMKDGER+
Sbjct: 41 GQYSAKLLKKRDQCRAVYACSHLFWVDDHDNMKDGERI 78

[Close]
BLAST of Lcy01g004470 vs. NCBI nr
Match: XP_023007615.1 (vacuolar protein sorting-associated protein 35A-like [Cucurbita maxima])

HSP 1 Score: 77.4 bits (189), Expect = 6.9e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 649 YSAKLLKKSDQCRAVYACSHLFWLDDHDNMKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. NCBI nr
Match: KAG7015827.1 (Vacuolar protein sorting-associated protein 35A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 77.4 bits (189), Expect = 6.9e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 676 YSAKLLKKSDQCRAVYACSHLFWLDDHDNMKDGERV 711

[Close]
BLAST of Lcy01g004470 vs. NCBI nr
Match: KAG6577787.1 (Vacuolar protein sorting-associated protein 35A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.4 bits (189), Expect = 6.9e-11
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSAKLLKK DQCRAVYAC HLFWLDDHDNMKDGER+
Sbjct: 649 YSAKLLKKSDQCRAVYACSHLFWLDDHDNMKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. TAIR 10
Match: AT1G75850.1 (VPS35 homolog B )

HSP 1 Score: 67.0 bits (162), Expect = 8.7e-12
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSA+LLKK DQCRAVYAC HLFW+DD D +KDGER+
Sbjct: 649 YSARLLKKPDQCRAVYACSHLFWVDDPDGIKDGERV 684

[Close]
BLAST of Lcy01g004470 vs. TAIR 10
Match: AT2G17790.1 (VPS35 homolog A )

HSP 1 Score: 64.3 bits (155), Expect = 5.6e-11
Identity = 26/36 (72.22%), Postives = 32/36 (88.89%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           Y+AKLLKK DQCRAVYAC HLFWL+D + ++DGER+
Sbjct: 649 YAAKLLKKPDQCRAVYACSHLFWLEDRETIQDGERV 684

[Close]
BLAST of Lcy01g004470 vs. TAIR 10
Match: AT3G51310.1 (VPS35 homolog C )

HSP 1 Score: 62.8 bits (151), Expect = 1.6e-10
Identity = 25/36 (69.44%), Postives = 31/36 (86.11%), Query Frame = 0 

Query: 26  YSAKLLKKLDQCRAVYACLHLFWLDDHDNMKDGERL 62
           YSA+LL+K DQCRAVY C HLFW D+ +N+KDGER+
Sbjct: 646 YSARLLRKPDQCRAVYECAHLFWADECENLKDGERV 681

[Close]
BLAST of Lcy01g004470 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 47.8 bits (112), Expect = 5.4e-06
Identity = 22/32 (68.75%), Postives = 25/32 (78.12%), Query Frame = 0 

Query: 1  MDGVSDFEPDCSEFVEVDPTGRYGRYSAKLLK 33
          M+ +S  EPD SEFVEVDPTGRYGRY+  L K
Sbjct: 1  MNNLSYLEPDYSEFVEVDPTGRYGRYNEVLGK 32

[Close]
BLAST of Lcy01g004470 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 6.0e-05
Identity = 20/32 (62.50%), Postives = 24/32 (75.00%), Query Frame = 0 

Query: 1  MDGVSDFEPDCSEFVEVDPTGRYGRYSAKLLK 33
          M+ +S  E D SE+VEVDPTGRYGRY+  L K
Sbjct: 2  MNNLSHLESDYSEYVEVDPTGRYGRYNEVLGK 33

The following BLAST results are available for this feature:
BLAST of Lcy01g004470 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4I0P81.2e-1077.78Vacuolar protein sorting-associated protein 35B OS=Arabidopsis thaliana OX=3702 ... [more]
Q7X6597.9e-1072.22Vacuolar protein sorting-associated protein 35A OS=Arabidopsis thaliana OX=3702 ... [more]
A8R7K92.3e-0969.44Vacuolar protein sorting-associated protein 35C OS=Arabidopsis thaliana OX=3702 ... [more]
Q9CAV67.7e-0568.75Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
P341105.0e-0452.94Vacuolar protein sorting-associated protein 35 OS=Saccharomyces cerevisiae (stra... [more]
BLAST of Lcy01g004470 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A4P1RIC96.7e-1286.84Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_22864 PE=3 S... [more]
A0A6J1L3G73.3e-1191.67Vacuolar protein sorting-associated protein 35 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1E6443.3e-1191.67Vacuolar protein sorting-associated protein 35 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5A7UCN04.4e-1191.67Vacuolar protein sorting-associated protein 35 OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0L6664.4e-1191.67Vacuolar protein sorting-associated protein 35 OS=Cucumis sativus OX=3659 GN=Csa... [more]
BLAST of Lcy01g004470 vs. NCBI nr
Match NameE-valueIdentityDescription
OIW11057.11.4e-1186.84hypothetical protein TanjilG_22864 [Lupinus angustifolius][more]
XP_019444852.11.4e-1186.84PREDICTED: aconitate hydratase-like isoform X1 [Lupinus angustifolius][more]
XP_023007615.16.9e-1191.67vacuolar protein sorting-associated protein 35A-like [Cucurbita maxima][more]
KAG7015827.16.9e-1191.67Vacuolar protein sorting-associated protein 35A [Cucurbita argyrosperma subsp. a... [more]
KAG6577787.16.9e-1191.67Vacuolar protein sorting-associated protein 35A, partial [Cucurbita argyrosperma... [more]
BLAST of Lcy01g004470 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G75850.18.7e-1277.78VPS35 homolog B [more]
AT2G17790.15.6e-1172.22VPS35 homolog A [more]
AT3G51310.11.6e-1069.44VPS35 homolog C [more]
AT3G04910.15.4e-0668.75with no lysine (K) kinase 1 [more]
AT5G28080.26.0e-0562.50Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005378Vacuolar protein sorting-associated protein 35PFAMPF03635Vps35coord: 25..62
e-value: 6.8E-9
score: 34.8
IPR005378Vacuolar protein sorting-associated protein 35PANTHERPTHR11099VACUOLAR SORTING PROTEIN 35coord: 25..62
IPR042491Vacuolar protein sorting-associated protein 35, C-terminalGENE3D1.25.40.660coord: 20..72
e-value: 3.5E-10
score: 41.5
NoneNo IPR availablePANTHERPTHR11099:SF3VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35Acoord: 25..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g004470.1Lcy01g004470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0042147 retrograde transport, endosome to Golgi
biological_process GO:0015031 protein transport
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005770 late endosome
cellular_component GO:0030906 retromer, cargo-selective complex