Lcy01g004450 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g004450
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionserine/threonine-protein kinase BLUS1-like
LocationChr01: 5968577 .. 5970127 (-)
RNA-Seq ExpressionLcy01g004450
SyntenyLcy01g004450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAGGTTGAAGATGAATTCCCAATGGAAGCCAAGGCCTACGAAATTCTGTGCGAGATCGGCGTAAGCGAACACACTATGGTTTACAAGGCGATCTGCGAGTCGATGAACTCCACGGTTGTTGCCGTCAAAGTTGTGGACATGGACGGATTGAACATGGGTGTCGACGAGTCTCATGCGAAGAACACGATAAACTCCCTCTCGCAGATCAACCACGCCAATATTCTCAGCCCCCACTGTTGTTTCACGGTGGATAATCGTCACCTCTGGATCGTAATGCCTTTCATCCATGCCGGATCTTTGGAGTCCGTAGCCGCCGCTCGTTTCCAGAACGGCTTACCGGGCCAATTCTTGGCCACAATCCTCCGCCAACTTCTCTCCGGCTTGGTTCATCTTCACCAACTCGGTCACGTTCATAAAAACATCAAGCCAAGTAACATTTTCCTCGATTCCGAAGCCACCATCAAGCTCTCGGATGTGAATGTTTTCAACCCAATAAAGGGTGCTTCCGATTCAGTACCATATTGGGAAGCGCCGGAACTAGGACGAGACTCCGCGGCTGACGAGTACAGTTTCAAATCTGATGTCTGGGCTGTTGGGGTTGTGGCGTTGGAATTAGCATCTGGATGCCTTCCTTTTACGGACTGGATGGTACTTATCGCCCAGTTCTTGAAGATTTCCCGGCGGTTCCCATACCGTATGGACCTGTATAACTTCGTGAGCAAGAATGCAGTGGACTGGAGATTCTCATTCTCGGACTCATTCAAGGACTTCGTGGGTTGTTGTCTTGTTAAAGATCCATCCGAGAGGGCTTCCGCGGCGAGTTTATCGGCGCATCCGTTCGTCGAGCTGGAGCGGGAGGGTACGGATTATGATATTGTATTGCTCTTGAAGAGTATTGTTAATGGAAAAGATATGGTTGGAGGGATCCAGGAAATGTTTCGAAAGAATGAAGATCGACTCAAGTGGTTAATGGATAAGAAGAGGACAGAGAGGTCCAGCATTGTAAATGACAGCATAGGAAAGACTAAGTCGTGGGAGTTCATCAACTGCAGCTTCAAACTCAAGGAAATCAAAAGAGATCATTCTTCCGAGAAGAAGGCGGAATCTGAATCCGAATCCGAATGGGAATCCGAACCGGAATCGGAATCGGAATCGGAAGAGAAGGAAGAAATAGTTGATGAAGCCAGAGTGATGAAGTCCTTGAGAGAAAGCTTAAACTGTGTGATTACTCAGTTTGGTGTGTTGTTTAAAAGAGAGTTGGAAATGGTGAGGGAAATAGAGAGTTTGGAGGGGGAGTTGGGGGTAGAGAAGGAGAGAAGCTTTGAGTTGGAGTTGGTAATGAGGAGGGAAATACAGAGGCTCAAGGCCGAGTTGGGGGTCGAGAAGGATCGAAACTTTCACTTAACCTTGGGTTACCGCTATTCCGAACCTCAAAAAGTTCAGAATTGACAAATTATTCAATGAGACAGTCACAATCTAGTTGTATACCCACTAGTTATTAGGGTATGTTATTGACACAACTAGAAGATATATGAAGGGAGGAAG

mRNA sequence

ATGGATCAGGTTGAAGATGAATTCCCAATGGAAGCCAAGGCCTACGAAATTCTGTGCGAGATCGGCGTAAGCGAACACACTATGGTTTACAAGGCGATCTGCGAGTCGATGAACTCCACGGTTGTTGCCGTCAAAGTTGTGGACATGGACGGATTGAACATGGGTGTCGACGAGTCTCATGCGAAGAACACGATAAACTCCCTCTCGCAGATCAACCACGCCAATATTCTCAGCCCCCACTGTTGTTTCACGGTGGATAATCGTCACCTCTGGATCGTAATGCCTTTCATCCATGCCGGATCTTTGGAGTCCGTAGCCGCCGCTCGTTTCCAGAACGGCTTACCGGGCCAATTCTTGGCCACAATCCTCCGCCAACTTCTCTCCGGCTTGGTTCATCTTCACCAACTCGGTCACGTTCATAAAAACATCAAGCCAAGTAACATTTTCCTCGATTCCGAAGCCACCATCAAGCTCTCGGATGTGAATGTTTTCAACCCAATAAAGGGTGCTTCCGATTCAGTACCATATTGGGAAGCGCCGGAACTAGGACGAGACTCCGCGGCTGACGAGTACAGTTTCAAATCTGATGTCTGGGCTGTTGGGGTTGTGGCGTTGGAATTAGCATCTGGATGCCTTCCTTTTACGGACTGGATGGTACTTATCGCCCAGTTCTTGAAGATTTCCCGGCGGTTCCCATACCGTATGGACCTGTATAACTTCGTGAGCAAGAATGCAGTGGACTGGAGATTCTCATTCTCGGACTCATTCAAGGACTTCGTGGGTTGTTGTCTTGTTAAAGATCCATCCGAGAGGGCTTCCGCGGCGAGTTTATCGGCGCATCCGTTCGTCGAGCTGGAGCGGGAGGGTACGGATTATGATATTGTATTGCTCTTGAAGAGTATTGTTAATGGAAAAGATATGGTTGGAGGGATCCAGGAAATGTTTCGAAAGAATGAAGATCGACTCAAGTGGTTAATGGATAAGAAGAGGACAGAGAGGTCCAGCATTGTAAATGACAGCATAGGAAAGACTAAGTCGTGGGAGTTCATCAACTGCAGCTTCAAACTCAAGGAAATCAAAAGAGATCATTCTTCCGAGAAGAAGGCGGAATCTGAATCCGAATCCGAATGGGAATCCGAACCGGAATCGGAATCGGAATCGGAAGAGAAGGAAGAAATAGTTGATGAAGCCAGAGTGATGAAGTCCTTGAGAGAAAGCTTAAACTGTGTGATTACTCAGTTTGGTGTGTTGTTTAAAAGAGAGTTGGAAATGGTGAGGGAAATAGAGAGTTTGGAGGGGGAGTTGGGGGTAGAGAAGGAGAGAAGCTTTGAGTTGGAGTTGGTAATGAGGAGGGAAATACAGAGGCTCAAGGCCGAGTTGGGGGTCGAGAAGGATCGAAACTTTCACTTAACCTTGGGTTACCGCTATTCCGAACCTCAAAAAGTTCAGAATTGACAAATTATTCAATGAGACAGTCACAATCTAGTTGTATACCCACTAGTTATTAGGGTATGTTATTGACACAACTAGAAGATATATGAAGGGAGGAAG

Coding sequence (CDS)

ATGGATCAGGTTGAAGATGAATTCCCAATGGAAGCCAAGGCCTACGAAATTCTGTGCGAGATCGGCGTAAGCGAACACACTATGGTTTACAAGGCGATCTGCGAGTCGATGAACTCCACGGTTGTTGCCGTCAAAGTTGTGGACATGGACGGATTGAACATGGGTGTCGACGAGTCTCATGCGAAGAACACGATAAACTCCCTCTCGCAGATCAACCACGCCAATATTCTCAGCCCCCACTGTTGTTTCACGGTGGATAATCGTCACCTCTGGATCGTAATGCCTTTCATCCATGCCGGATCTTTGGAGTCCGTAGCCGCCGCTCGTTTCCAGAACGGCTTACCGGGCCAATTCTTGGCCACAATCCTCCGCCAACTTCTCTCCGGCTTGGTTCATCTTCACCAACTCGGTCACGTTCATAAAAACATCAAGCCAAGTAACATTTTCCTCGATTCCGAAGCCACCATCAAGCTCTCGGATGTGAATGTTTTCAACCCAATAAAGGGTGCTTCCGATTCAGTACCATATTGGGAAGCGCCGGAACTAGGACGAGACTCCGCGGCTGACGAGTACAGTTTCAAATCTGATGTCTGGGCTGTTGGGGTTGTGGCGTTGGAATTAGCATCTGGATGCCTTCCTTTTACGGACTGGATGGTACTTATCGCCCAGTTCTTGAAGATTTCCCGGCGGTTCCCATACCGTATGGACCTGTATAACTTCGTGAGCAAGAATGCAGTGGACTGGAGATTCTCATTCTCGGACTCATTCAAGGACTTCGTGGGTTGTTGTCTTGTTAAAGATCCATCCGAGAGGGCTTCCGCGGCGAGTTTATCGGCGCATCCGTTCGTCGAGCTGGAGCGGGAGGGTACGGATTATGATATTGTATTGCTCTTGAAGAGTATTGTTAATGGAAAAGATATGGTTGGAGGGATCCAGGAAATGTTTCGAAAGAATGAAGATCGACTCAAGTGGTTAATGGATAAGAAGAGGACAGAGAGGTCCAGCATTGTAAATGACAGCATAGGAAAGACTAAGTCGTGGGAGTTCATCAACTGCAGCTTCAAACTCAAGGAAATCAAAAGAGATCATTCTTCCGAGAAGAAGGCGGAATCTGAATCCGAATCCGAATGGGAATCCGAACCGGAATCGGAATCGGAATCGGAAGAGAAGGAAGAAATAGTTGATGAAGCCAGAGTGATGAAGTCCTTGAGAGAAAGCTTAAACTGTGTGATTACTCAGTTTGGTGTGTTGTTTAAAAGAGAGTTGGAAATGGTGAGGGAAATAGAGAGTTTGGAGGGGGAGTTGGGGGTAGAGAAGGAGAGAAGCTTTGAGTTGGAGTTGGTAATGAGGAGGGAAATACAGAGGCTCAAGGCCGAGTTGGGGGTCGAGAAGGATCGAAACTTTCACTTAACCTTGGGTTACCGCTATTCCGAACCTCAAAAAGTTCAGAATTGA

Protein sequence

MDQVEDEFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTINSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHSSEKKAESESESEWESEPESESESEEKEEIVDEARVMKSLRESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELELVMRREIQRLKAELGVEKDRNFHLTLGYRYSEPQKVQN
Homology
BLAST of Lcy01g004450 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.4e-52
Identity = 159/501 (31.74%), Postives = 264/501 (52.69%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTIN 66
           EFP++A+AYEI+C+IGV     VYKAIC  MNS VVA+K +D+D      D    +    
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLD--QSRADFDSLRRETK 66

Query: 67  SLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQL 126
           ++S ++H NIL+ +C FTVD R LW+VMPF+  GSL S+ ++ F +GLP   ++  L++ 
Sbjct: 67  TMSLLSHPNILNAYCSFTVD-RCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKET 126

Query: 127 LSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSD----VNVFNPIKGASDS--------- 186
           L+ + +LH  GH+H++IK  NI +DS+ ++KL+D     +++ P+  +S +         
Sbjct: 127 LNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTD 186

Query: 187 ---VPYWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRR 246
               PYW APE+        Y FK+D+W+ G+ ALELA G  P +    L +  +KI++R
Sbjct: 187 IAGTPYWMAPEVVHSHTG--YGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKR 246

Query: 247 FPYRMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGT 306
           F +  D     S ++      FS +F++ VG CL +DP++R SA  L  HPF +   +G 
Sbjct: 247 FHF-SDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFK-NCKGL 306

Query: 307 DYDIVLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMD---KKRTERSSIVNDSIGKTKSW 366
           D+ +  +L S+ N        ++MF +++  +K + D   ++  E   IV +   +   W
Sbjct: 307 DFVVKNVLHSLSNA-------EQMFMESQILIKSVGDDDEEEEEEDEEIVKNR--RISGW 366

Query: 367 EFINCSFKLKEI---KRDHSSEKKAESESESEWESEPES---------------ESESEE 426
            F     +L  +       SSE     E +S+ + E ++               E++++E
Sbjct: 367 NFREDDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQE 426

Query: 427 KEEI-VDEARVMKSLRESLNCVITQFGVLFKRELEMVRE-----IESLEGELGVEKERSF 465
            E +  D+  V++ L+            + K+ LE  R      IE+L G+   ++E+S 
Sbjct: 427 GEVVGFDKDLVLEKLK------------VLKKSLEHQRARVSIIIEALSGD---KEEKSR 474

BLAST of Lcy01g004450 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 91/302 (30.13%), Postives = 146/302 (48.34%), Query Frame = 0

Query: 8   FPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGL-NMGVDESHAKNTIN 67
           +P  A  YE+   IG     +V +AIC       VA+K++D++   N+ ++E   +  I 
Sbjct: 64  YPNSADQYELKETIGKGGSGLVQRAICLPFQEN-VAIKIIDLEHCKNVSLEE--IRKEIQ 123

Query: 68  SLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQL 127
           ++S  +H N+++ H  F V N  LW++M F+ AGS   +    F  G     +ATIL++ 
Sbjct: 124 AMSLCHHPNVVAYHTSF-VYNESLWVIMDFLSAGSCSDIMRFSFPQGFEEHVIATILKEA 183

Query: 128 LSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDS-------VPYWEA 187
           L  + + H+ G +H++IK  NI +DS   I+LSD  V   +    ++        P W A
Sbjct: 184 LKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLIDTGETSRNTFVGTPCWMA 243

Query: 188 PELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYN 247
           PE+        Y +  D+W+ G+ ALELA G  PF ++  +    L +    P       
Sbjct: 244 PEIMEQV---NYDYAVDIWSFGITALELARGKAPFAEYPPMKVLLLTLQNPPP------- 303

Query: 248 FVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLK 302
                  D    +S SFKD V  CL KDPS+R   + L  H F + + +  DY +  +L 
Sbjct: 304 ---SLEGDGESKWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFK-QAKKPDYLVQHILA 347

BLAST of Lcy01g004450 vs. ExPASy Swiss-Prot
Match: Q86IX1 (Serine/threonine-protein kinase dst1 OS=Dictyostelium discoideum OX=44689 GN=dst1 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.0e-27
Identity = 88/300 (29.33%), Postives = 150/300 (50.00%), Query Frame = 0

Query: 15  YEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTINSLSQINHA 74
           Y I   +G      V+KA+    N  VVA+K++ +D      D    +  I+ L++ N+ 
Sbjct: 29  YHIQERLGKGSFGQVFKAV-HFANGKVVAIKIISLDDQEAIKD---VRKEISILAECNYP 88

Query: 75  NILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQLLSGLVHLH 134
           NI+     +  D++ LWIVM +   GS+  +   +  + +    +A I R+ L GL +LH
Sbjct: 89  NIVQYFGSYFKDHQ-LWIVMEYCGGGSVSDL--LQVIDTISEDEIALICREALKGLNYLH 148

Query: 135 QLGHVHKNIKPSNIFLDSEATIKLSDVNV----FNPIKGASDSV--PYWEAPELGRDSAA 194
           +   +H++IK  NI L+    +KL+D  V    FN     +  V  PYW APE+ ++   
Sbjct: 149 EFKKIHRDIKGGNILLNDRGEVKLADFGVSAQLFNTFSKRNTFVGTPYWMAPEVIQE--- 208

Query: 195 DEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNAVDW 254
           ++Y  K+DVW++G+ A+E+A G  P ++   +   F+      P   D            
Sbjct: 209 NKYDGKADVWSLGITAIEMAEGLPPNSNVHPMRVIFMIPREESPALTD------------ 268

Query: 255 RFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELERE-GTDYDIVLLLKSIVNGKDM 308
           +  +SD F+DF+  CL KDP+ER +A  L  H F++ ++      D+V   K+++N   M
Sbjct: 269 KSIWSDKFQDFISKCLTKDPAERPTAKELLNHEFIQTKKPLSILSDLVENCKTLINNSSM 306

BLAST of Lcy01g004450 vs. ExPASy Swiss-Prot
Match: G5EEN4 (Germinal center kinase 3 OS=Caenorhabditis elegans OX=6239 GN=gck-3 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 7.3e-25
Identity = 107/426 (25.12%), Postives = 187/426 (43.90%), Query Frame = 0

Query: 6   DEFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTI 65
           D +P E  AY++   IGV     V+ A C   N   VA+K ++++     VDE    + I
Sbjct: 99  DRWPTEPSAYKLDESIGVGATATVFTAYCLPRNEK-VAIKCINLEKCQTSVDE--LSHEI 158

Query: 66  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSL--------ESVAAARFQNGLPGQ 125
            ++SQ NH N++S +  F +    LW+VM  ++ GS+        +++   + Q G+  +
Sbjct: 159 QAMSQCNHPNVVSYYTSF-IAQEELWVVMRLLNCGSMLDILKRKVKAIGKEQAQFGVLDE 218

Query: 126 F-LATILRQLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSV-- 185
             +AT+LR++L GL + H  G +H++IK  NI L  + TI+++D  V   +  +   +  
Sbjct: 219 VSIATVLREVLKGLEYFHLNGQIHRDIKAGNILLADDGTIQIADFGVSGWLASSGGDLSR 278

Query: 186 ----------PYWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQF 245
                     P W APE+        Y FK+D+W++G++A+ELA+G  P+  +  +    
Sbjct: 279 QKVRHTFVGTPCWMAPEVMEQ--VQGYDFKADIWSLGILAIELATGTAPYHKYPPMKVLM 338

Query: 246 LKISRRFPYRMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVE 305
           L +    P         +    D   ++  SFK  +  CL KDP++R +A+ L  + F  
Sbjct: 339 LTLQNDPPTLE-----TNAERKDQYKAYGKSFKTLIRDCLQKDPAKRPTASELLKYSF-- 398

Query: 306 LEREGTDYDIVLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKT 365
                                         F+K +D+ K+L+       +S+        
Sbjct: 399 ------------------------------FKKGKDK-KYLVHTLIENLASV-------- 458

Query: 366 KSWEFINCSFKLKEIKRDHSSEK----KAESESESEWESEPESESESEEKEEIVDEARVM 407
                         +   HSS+K    K   ++   WE E +S  ES++  ++ DE R  
Sbjct: 459 -------------PVVAHHSSKKVASGKLRKDAHGNWEFEYDSPQESDDDSDLEDEEREK 459

BLAST of Lcy01g004450 vs. ExPASy Swiss-Prot
Match: P83510 (Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 1.6e-24
Identity = 133/484 (27.48%), Postives = 216/484 (44.63%), Query Frame = 0

Query: 12  AKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTINSLSQI 71
           A  +E++  +G   +  VYK         + A+KV+D+     G +E   K  IN L + 
Sbjct: 22  AGIFELVELVGNGTYGQVYKG-RHVKTGQLAAIKVMDV----TGDEEEEIKQEINMLKKY 81

Query: 72  -NHANILSPHCCFTVDN-----RHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 131
            +H NI + +  F   N       LW+VM F  AGS+  +      N L  +++A I R+
Sbjct: 82  SHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICRE 141

Query: 132 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPI------KGASDSVPYWEA 191
           +L GL HLHQ   +H++IK  N+ L   A +KL D  V   +      +      PYW A
Sbjct: 142 ILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMA 201

Query: 192 PELGRDSAADE-----YSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYR 251
           PE+    A DE     Y FKSD+W++G+ A+E+A G  P  D   + A FL I R    R
Sbjct: 202 PEV---IACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFL-IPRNPAPR 261

Query: 252 MDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDI 311
           +      SK        +S  F+ F+  CLVK+ S+R +   L  HPF+    +  +  +
Sbjct: 262 LK-----SK-------KWSKKFQSFIESCLVKNHSQRPATEQLMKHPFI--RDQPNERQV 321

Query: 312 VLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTER------SSIVNDSIGKTKSWE 371
            + LK  ++      G      K+E   ++   ++  E       SSI+N     T   +
Sbjct: 322 RIQLKDHIDRTKKKRG-----EKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRD 381

Query: 372 FINCSFKLKE----IKRDHSSEKKAESESESEW---ESEPESESESEEKEEIVDEARVMK 431
           F+      KE    ++R    +++ E+E        E +   E + E++  + ++ R  K
Sbjct: 382 FLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREK 441

Query: 432 SLRESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELELVMRREIQRLKAELG 466
            LR+       +       E +M RE E    E   E +R    +L  +R+ +RL+ +L 
Sbjct: 442 ELRKQQEREQRR-----HYEEQMRREEERRRAEHEQEYKRK---QLEEQRQAERLQRQLK 469

BLAST of Lcy01g004450 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 2.7e-91
Identity = 232/504 (46.03%), Postives = 311/504 (61.71%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESM-NSTVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  Y+IL +IGVS+H++VYKAICES  NS  VAVKV+D+D     VDE      +
Sbjct: 22  QFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDVD---RHVDEFFVMK-M 81

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
           NSLS I+HANILSPHCCFTVD R LW VMPFIHAG L+++ A RF NGLP QFLA +L Q
Sbjct: 82  NSLSLIDHANILSPHCCFTVD-RRLWTVMPFIHAGPLQAILANRFPNGLPDQFLAILLHQ 141

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD-SVPYWEAPELGR 186
           LL  L+ LH+LG+VH+NIK SNIF+DSE ++KLSD+NV+       D SVPYW+ P+   
Sbjct: 142 LLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVGDDDDDDSSVPYWDPPD--- 201

Query: 187 DSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKN 246
               +EYSFKSDVWA+G+VALE+A GC P +D   + +Q L ISRRFPY ++L    SK+
Sbjct: 202 ----EEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYNLNLDKMSSKS 261

Query: 247 AVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNG 306
            +D +  F + FKDFV  CLVKDPS+R SAASL AHPF+   ++G   ++  L KSI   
Sbjct: 262 VIDRK--FGELFKDFVSSCLVKDPSKRPSAASLLAHPFI---KKGETSNV--LFKSI--A 321

Query: 307 KDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINCSFKLK------- 366
           KDM  GI++ F KN+DRL  LM K R    +    ++ K   W+F N  F+L+       
Sbjct: 322 KDM-AGIEDRFEKNKDRLVELMKKDRRVEDN-NRGNVVKVSGWDFNNFLFELEPDEESLG 381

Query: 367 -----------EIKRDHSSEKKA-------------ESESESEWESEPESESESEEKEEI 426
                       ++ DH S  +A               +S S         S +EE+  +
Sbjct: 382 KRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSRAEEEVRV 441

Query: 427 VDE---ARVMKSLRESLN-------CVITQFGVLFKRELEMVREIESLEGELGVEKERSF 468
           +D    A+V+ SL ES++        VI+QFG L +R++EM +EI+ L+ EL  EKERSF
Sbjct: 442 MDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELAYEKERSF 500

BLAST of Lcy01g004450 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-76
Identity = 186/467 (39.83%), Postives = 280/467 (59.96%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNS-TVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S ++ T+VA+K++D    N+ +D++   N  
Sbjct: 20  KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIIDS---NVEIDQTAITNIN 79

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +IL+PHCCFT  +  LWIVMPFI AG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 80  TNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFRTGLPDQFMSILINQ 139

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK +K SNIF D+   + LSD++V   +K    SVPYW+ PE    
Sbjct: 140 LLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMKKDCSSVPYWDPPE---- 199

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YSFKSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L N   K+ 
Sbjct: 200 ---EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDNRSGKSV 259

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KD   RASA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 260 VPRK--FGDTLKHFVGSCLAKDRFARASALSLLDHPFVK-KGENSNF----LFKSIAKG- 319

Query: 307 DMVGGIQEMFRKNEDRLKWLM-DKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHS 366
             + GI EMF +++DR+  LM ++ +   SS     + +   W++ N  F+L+  +    
Sbjct: 320 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPDEESFG 379

Query: 367 SEKKAESESESEWESEPESE----------SESEEKEEIVDEARVMKSL----------- 426
              +   E+   ++ + +              S+EK++ VD+A   ++L           
Sbjct: 380 KRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTETLATLLSSLLDNI 439

Query: 427 ---RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
              +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 440 DTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Lcy01g004450 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 1.8e-74
Identity = 190/468 (40.60%), Postives = 277/468 (59.19%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNST-VVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S +ST +VA+K++D    N+ +D +   N  
Sbjct: 20  KFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIKIIDS---NVEIDPTAITNIN 79

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +ILSPHCCF  ++  LWIVMPFIHAG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 80  TNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQRFRTGLPDQFMSILINQ 139

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK++K SNIF D+   + LSD++V   +     SVPYW  PE    
Sbjct: 140 LLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVSKDMNKDCSSVPYWVPPE---- 199

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YS KSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L     K+ 
Sbjct: 200 ---EDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDTRSGKSV 259

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KDP  R SA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 260 VPRK--FGDTLKHFVGSCLAKDPFARVSALSLLDHPFVK-KGENSNF----LFKSIAKG- 319

Query: 307 DMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKT---KSWEFINCSFKL------ 366
             + GI EMF +++DR+  LM  +     S      GK      W++ N  F+L      
Sbjct: 320 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYNNFLFELEPDEES 379

Query: 367 --KEIK-RDHSSEKKAESESESEWESEPESESESEEKEEIVDEARVMKSL---------- 426
             K ++ R  +     E E  ++++ +      S+EK+E VD+A   ++L          
Sbjct: 380 FGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSDEKKE-VDQAMDSETLATLLSSLLDN 439

Query: 427 ----RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
               +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 440 IDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Lcy01g004450 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-60
Identity = 176/492 (35.77%), Postives = 263/492 (53.46%), Query Frame = 0

Query: 8   FPMEAKAYEILCEIGVSEHTMVYKAICESMNST-----VVAVKVVDMDGLNMGVDESHAK 67
           FP  +  Y IL +IG S ++ VY A C+S +S+      VA+K+++   +   + +++  
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQI---IFDTNLL 62

Query: 68  NTINSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATI 127
           NT  SL+ I+H NI+ PHCCF  D+  LWIVMP++ AG L+SV +  F  G+P QF + +
Sbjct: 63  NT--SLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSIL 122

Query: 128 LRQLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD---SVPYWEA 187
           L Q+L  LV+LH++G VHK IKPSNIF DS+ T+KLS  ++        D   S+PYW  
Sbjct: 123 LSQVLQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISADNKIDRDSSIPYWVP 182

Query: 188 PELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYN 247
           P+       DEYSFKSD+W VGV+A+E+  G  P  D  +L +Q L ISRRFPY +    
Sbjct: 183 PD-------DEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKT-E 242

Query: 248 FVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLK 307
              +N+ D R  F D+ K+FV  CL+KD  ER +AASL  HPFV       ++    L K
Sbjct: 243 TGRRNSGDRRI-FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNF----LFK 302

Query: 308 SIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINCSFKLK-- 367
           SI  G   V  I   F+  ++R+  +M+     ++   ++ I K   W + +  F+L+  
Sbjct: 303 SIPKG---VIEIDRRFQLEKERIIGIMNNDEGLKNQ--SEKITKISGWVYNDFLFELEPD 362

Query: 368 ------------EIKRDHSSEKKAESESESE--------------------WESEPESES 427
                       E  R+   E++ E E ES                      +   ES  
Sbjct: 363 GESLGKRVRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSK 422

Query: 428 ESEEKEEIVDEARVMKSL----------RESLNCVITQFGVLFKRELEMVREIESLEGEL 448
           + ++K++IVD+  ++K L          RE ++ +I   G L +++ EM  EI+ LE EL
Sbjct: 423 KKKKKKKIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETEL 471

BLAST of Lcy01g004450 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-60
Identity = 176/492 (35.77%), Postives = 263/492 (53.46%), Query Frame = 0

Query: 8   FPMEAKAYEILCEIGVSEHTMVYKAICESMNST-----VVAVKVVDMDGLNMGVDESHAK 67
           FP  +  Y IL +IG S ++ VY A C+S +S+      VA+K+++   +   + +++  
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQI---IFDTNLL 62

Query: 68  NTINSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATI 127
           NT  SL+ I+H NI+ PHCCF  D+  LWIVMP++ AG L+SV +  F  G+P QF + +
Sbjct: 63  NT--SLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSIL 122

Query: 128 LRQLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD---SVPYWEA 187
           L Q+L  LV+LH++G VHK IKPSNIF DS+ T+KLS  ++        D   S+PYW  
Sbjct: 123 LSQVLQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISADNKIDRDSSIPYWVP 182

Query: 188 PELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYN 247
           P+       DEYSFKSD+W VGV+A+E+  G  P  D  +L +Q L ISRRFPY +    
Sbjct: 183 PD-------DEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKT-E 242

Query: 248 FVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLK 307
              +N+ D R  F D+ K+FV  CL+KD  ER +AASL  HPFV       ++    L K
Sbjct: 243 TGRRNSGDRRI-FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNF----LFK 302

Query: 308 SIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINCSFKLK-- 367
           SI  G   V  I   F+  ++R+  +M+     ++   ++ I K   W + +  F+L+  
Sbjct: 303 SIPKG---VIEIDRRFQLEKERIIGIMNNDEGLKNQ--SEKITKISGWVYNDFLFELEPD 362

Query: 368 ------------EIKRDHSSEKKAESESESE--------------------WESEPESES 427
                       E  R+   E++ E E ES                      +   ES  
Sbjct: 363 GESLGKRVRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSK 422

Query: 428 ESEEKEEIVDEARVMKSL----------RESLNCVITQFGVLFKRELEMVREIESLEGEL 448
           + ++K++IVD+  ++K L          RE ++ +I   G L +++ EM  EI+ LE EL
Sbjct: 423 KKKKKKKIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETEL 471

BLAST of Lcy01g004450 vs. NCBI nr
Match: XP_022138626.1 (serine/threonine-protein kinase BLUS1-like [Momordica charantia])

HSP 1 Score: 345.9 bits (886), Expect = 5.6e-91
Identity = 232/504 (46.03%), Postives = 311/504 (61.71%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESM-NSTVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  Y+IL +IGVS+H++VYKAICES  NS  VAVKV+D+D     VDE      +
Sbjct: 22  QFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDVD---RHVDEFFVMK-M 81

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
           NSLS I+HANILSPHCCFTVD R LW VMPFIHAG L+++ A RF NGLP QFLA +L Q
Sbjct: 82  NSLSLIDHANILSPHCCFTVD-RRLWTVMPFIHAGPLQAILANRFPNGLPDQFLAILLHQ 141

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD-SVPYWEAPELGR 186
           LL  L+ LH+LG+VH+NIK SNIF+DSE ++KLSD+NV+       D SVPYW+ P+   
Sbjct: 142 LLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVGDDDDDDSSVPYWDPPD--- 201

Query: 187 DSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKN 246
               +EYSFKSDVWA+G+VALE+A GC P +D   + +Q L ISRRFPY ++L    SK+
Sbjct: 202 ----EEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYNLNLDKMSSKS 261

Query: 247 AVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNG 306
            +D +  F + FKDFV  CLVKDPS+R SAASL AHPF+   ++G   ++  L KSI   
Sbjct: 262 VIDRK--FGELFKDFVSSCLVKDPSKRPSAASLLAHPFI---KKGETSNV--LFKSI--A 321

Query: 307 KDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINCSFKLK------- 366
           KDM  GI++ F KN+DRL  LM K R    +    ++ K   W+F N  F+L+       
Sbjct: 322 KDM-AGIEDRFEKNKDRLVELMKKDRRVEDN-NRGNVVKVSGWDFNNFLFELEPDEESLG 381

Query: 367 -----------EIKRDHSSEKKA-------------ESESESEWESEPESESESEEKEEI 426
                       ++ DH S  +A               +S S         S +EE+  +
Sbjct: 382 KRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSRAEEEVRV 441

Query: 427 VDE---ARVMKSLRESLN-------CVITQFGVLFKRELEMVREIESLEGELGVEKERSF 468
           +D    A+V+ SL ES++        VI+QFG L +R++EM +EI+ L+ EL  EKERSF
Sbjct: 442 MDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELAYEKERSF 500

BLAST of Lcy01g004450 vs. NCBI nr
Match: KAG7013499.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 297.7 bits (761), Expect = 1.8e-76
Identity = 189/468 (40.38%), Postives = 282/468 (60.26%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNS-TVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S ++ T+VA+K++D    N+ +D++   N  
Sbjct: 26  KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIIDS---NVEIDQTAITNIN 85

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +IL+PHCCFT  +  LWIVMPFI AG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 86  TNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFRTGLPDQFMSILINQ 145

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK +K SNIF D+   + LSD++V   +K    SVPYW+ PE    
Sbjct: 146 LLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMKKDCSSVPYWDPPE---- 205

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YSFKSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L N   K+ 
Sbjct: 206 ---EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDNRSGKSV 265

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KD   RASA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 266 VPRK--FGDTLKHFVGSCLAKDRFARASALSLLDHPFVK-KGENSNF----LFKSIAKG- 325

Query: 307 DMVGGIQEMFRKNEDRLKWLM-DKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHS 366
             + GI EMF +++DR+  LM ++ +   SS     + +   W++ N  F+L+  +    
Sbjct: 326 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPDEESFG 385

Query: 367 -----------SEKKAESESESEWESEPESESESEEKEEIVDEARVMKSL---------- 426
                      S K+ E  ++ + +S       S+EK++ VD+A   ++L          
Sbjct: 386 KRVRFRGETVFSYKEQEQCNKFDMKSLMHGGGSSDEKKKEVDQAMDTETLATLLSSLLDN 445

Query: 427 ----RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
               +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 446 IDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 473

BLAST of Lcy01g004450 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 297.4 bits (760), Expect = 2.3e-76
Identity = 186/467 (39.83%), Postives = 280/467 (59.96%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNS-TVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S ++ T+VA+K++D    N+ +D++   N  
Sbjct: 20  KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIIDS---NVEIDQTAITNIN 79

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +IL+PHCCFT  +  LWIVMPFI AG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 80  TNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFRTGLPDQFMSILINQ 139

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK +K SNIF D+   + LSD++V   +K    SVPYW+ PE    
Sbjct: 140 LLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMKKDCSSVPYWDPPE---- 199

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YSFKSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L N   K+ 
Sbjct: 200 ---EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDNRSGKSV 259

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KD   RASA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 260 VPRK--FGDTLKHFVGSCLAKDRFARASALSLLDHPFVK-KGENSNF----LFKSIAKG- 319

Query: 307 DMVGGIQEMFRKNEDRLKWLM-DKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHS 366
             + GI EMF +++DR+  LM ++ +   SS     + +   W++ N  F+L+  +    
Sbjct: 320 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPDEESFG 379

Query: 367 SEKKAESESESEWESEPESE----------SESEEKEEIVDEARVMKSL----------- 426
              +   E+   ++ + +              S+EK++ VD+A   ++L           
Sbjct: 380 KRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTETLATLLSSLLDNI 439

Query: 427 ---RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
              +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 440 DTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Lcy01g004450 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.4 bits (760), Expect = 2.3e-76
Identity = 186/467 (39.83%), Postives = 280/467 (59.96%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNS-TVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S ++ T+VA+K++D    N+ +D++   N  
Sbjct: 20  KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIIDS---NVEIDQTAITNIN 79

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +IL+PHCCFT  +  LWIVMPFI AG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 80  TNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFRTGLPDQFMSILINQ 139

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK +K SNIF D+   + LSD++V   +K    SVPYW+ PE    
Sbjct: 140 LLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMKKDCSSVPYWDPPE---- 199

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YSFKSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L N   K+ 
Sbjct: 200 ---EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDNRSGKSV 259

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KD   RASA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 260 VPRK--FGDTLKHFVGSCLAKDRFARASALSLLDHPFVK-KGENSNF----LFKSIAKG- 319

Query: 307 DMVGGIQEMFRKNEDRLKWLM-DKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHS 366
             + GI EMF +++DR+  LM ++ +   SS     + +   W++ N  F+L+  +    
Sbjct: 320 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPDEESFG 379

Query: 367 SEKKAESESESEWESEPESE----------SESEEKEEIVDEARVMKSL----------- 426
              +   E+   ++ + +              S+EK++ VD+A   ++L           
Sbjct: 380 KRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTETLATLLSSLLDNI 439

Query: 427 ---RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
              +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 440 DTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Lcy01g004450 vs. NCBI nr
Match: XP_023548299.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296.6 bits (758), Expect = 3.9e-76
Identity = 186/466 (39.91%), Postives = 279/466 (59.87%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNS-TVVAVKVVDMDGLNMGVDESHAKNTI 66
           +FP  A  YEI  +IG S+H+ VYKAIC+S ++ T+VA+K++D    N+ +D +   N  
Sbjct: 26  KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIIDS---NVEIDPTAITNIN 85

Query: 67  NSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQ 126
            +LS INH +IL+PHCCFT  +  LWIVMPFIHAG L+++ + RF+ GLP QF++ ++ Q
Sbjct: 86  TNLSFINHPHILTPHCCFTAPDGRLWIVMPFIHAGPLQAIISQRFRTGLPDQFMSILINQ 145

Query: 127 LLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASDSVPYWEAPELGRD 186
           LL  LV LH+ G VHK +K SNIF D+   + LSD++V   +     SVPYW+ PE    
Sbjct: 146 LLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSKEMNKDCSSVPYWDPPE---- 205

Query: 187 SAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPYRMDLYNFVSKNA 246
              ++YSFKSDVW+VG++A+E+A GC P  D  V+ AQF KISRRFPY ++L N   K+ 
Sbjct: 206 ---EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRRFPYDLNLDNRSGKSV 265

Query: 247 VDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDIVLLLKSIVNGK 306
           V  +  F D+ K FVG CL KD   RASA SL  HPFV+ + E +++    L KSI  G 
Sbjct: 266 VPRK--FGDTLKHFVGSCLAKDRFARASALSLLDHPFVK-KGENSNF----LFKSIAKG- 325

Query: 307 DMVGGIQEMFRKNEDRLKWLM-DKKRTERSSIVNDSIGKTKSWEFINCSFKLKEIKRDHS 366
             + GI EMF +++DR+  LM ++ +   SS     + +   W++ N  F+L+  +    
Sbjct: 326 --MVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPDEESFG 385

Query: 367 SEKKAESESESEWESEPE-------------SESESEEKEEIVDE---ARVMKSL----- 426
              +   E+   ++ + +             S  E +E ++ +D    A ++ SL     
Sbjct: 386 KRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKEVDQAMDSETLATLLSSLLDNID 445

Query: 427 --RESLNCVITQFGVLFKRELEMVREIESLEGELGVEKERSFELEL 448
             +E ++ ++   G +  ++LEMV++I+ LE EL  EKE+SF+LEL
Sbjct: 446 TQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 471

BLAST of Lcy01g004450 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-53
Identity = 159/501 (31.74%), Postives = 264/501 (52.69%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTIN 66
           EFP++A+AYEI+C+IGV     VYKAIC  MNS VVA+K +D+D      D    +    
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLD--QSRADFDSLRRETK 66

Query: 67  SLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQL 126
           ++S ++H NIL+ +C FTVD R LW+VMPF+  GSL S+ ++ F +GLP   ++  L++ 
Sbjct: 67  TMSLLSHPNILNAYCSFTVD-RCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKET 126

Query: 127 LSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSD----VNVFNPIKGASDS--------- 186
           L+ + +LH  GH+H++IK  NI +DS+ ++KL+D     +++ P+  +S +         
Sbjct: 127 LNAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTD 186

Query: 187 ---VPYWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRR 246
               PYW APE+        Y FK+D+W+ G+ ALELA G  P +    L +  +KI++R
Sbjct: 187 IAGTPYWMAPEVVHSHTG--YGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKR 246

Query: 247 FPYRMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGT 306
           F +  D     S ++      FS +F++ VG CL +DP++R SA  L  HPF +   +G 
Sbjct: 247 FHF-SDYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFK-NCKGL 306

Query: 307 DYDIVLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMD---KKRTERSSIVNDSIGKTKSW 366
           D+ +  +L S+ N        ++MF +++  +K + D   ++  E   IV +   +   W
Sbjct: 307 DFVVKNVLHSLSNA-------EQMFMESQILIKSVGDDDEEEEEEDEEIVKNR--RISGW 366

Query: 367 EFINCSFKLKEI---KRDHSSEKKAESESESEWESEPES---------------ESESEE 426
            F     +L  +       SSE     E +S+ + E ++               E++++E
Sbjct: 367 NFREDDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQE 426

Query: 427 KEEI-VDEARVMKSLRESLNCVITQFGVLFKRELEMVRE-----IESLEGELGVEKERSF 465
            E +  D+  V++ L+            + K+ LE  R      IE+L G+   ++E+S 
Sbjct: 427 GEVVGFDKDLVLEKLK------------VLKKSLEHQRARVSIIIEALSGD---KEEKSR 474

BLAST of Lcy01g004450 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 3.0e-42
Identity = 130/419 (31.03%), Postives = 203/419 (48.45%), Query Frame = 0

Query: 5   EDEFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNT 64
           E +FP+ AK Y++  EIG      V++A+C  +N  VVA+KV+D++  N  +D    +  
Sbjct: 6   EKKFPLNAKDYKLYEEIGDGVSATVHRALCIPLN-VVVAIKVLDLEKCNNDLD--GIRRE 65

Query: 65  INSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILR 124
           + ++S INH N+L  HC FT  ++ LW+VMP++  GS   +  + + +G     +AT+LR
Sbjct: 66  VQTMSLINHPNVLQAHCSFTTGHQ-LWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLLR 125

Query: 125 QLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD---------SVP 184
           + L  LV+LH  GH+H+++K  NI LDS   +KL+D  V   +    D           P
Sbjct: 126 ETLKALVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQRSRNTFVGTP 185

Query: 185 YWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDW---MVLIAQFLKISRRFP 244
            W APE+ +      Y FK+DVW+ G+ ALELA G  PF+ +    VL+           
Sbjct: 186 CWMAPEVMQQ--LHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPPGLD 245

Query: 245 YRMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDY 304
           Y  D               FS +FK+ VG CLVKDP +R ++  L  HPF +  R   DY
Sbjct: 246 YERDK-------------RFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARP-ADY 305

Query: 305 DIVLLLKSIVNGKDMVGGIQEMFRKNEDRLKWLMDKKRTERSSIVNDSIGKTKSWEFINC 364
               L+K+I+NG   +G      +  E  L  + +K   E      + I    +W     
Sbjct: 306 ----LVKTILNGLPPLGDRYRQIKSKEADL-LMQNKSEYEAHLSQQEYIRGISAW----- 365

Query: 365 SFKLKEIKRDH---SSEKKAESESESEWESEPESESESEEKEEIVDEARVMKSLRESLN 409
           +F L+++K      S +  + +E     + + E + ES    E    +    S  + LN
Sbjct: 366 NFNLEDLKTQAALISDDDTSHAEEPDFNQKQCERQDESALSPERASSSATAPSQDDELN 394

BLAST of Lcy01g004450 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-39
Identity = 110/358 (30.73%), Postives = 176/358 (49.16%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTIN 66
           ++P+  + Y +   IG     +V++A+C   +  VVA+K++D +  N   D ++      
Sbjct: 8   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDN--CDLNNISREAQ 67

Query: 67  SLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQL 126
           ++  ++H N+L  HC F V + +LW++MP++  GS   +  A + +G     +ATILR+ 
Sbjct: 68  TMMLVDHPNVLKSHCSF-VSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 127

Query: 127 LSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD---------SVPYW 186
           L GL +LHQ GH+H+++K  NI L +   +KL D  V   +  + D           P W
Sbjct: 128 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCW 187

Query: 187 EAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDW---MVLIAQFLKISRRFPYR 246
            APE+        Y FK+D+W+ G+  LELA G  PF+ +    VL+           Y 
Sbjct: 188 MAPEVMEQ--LHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYE 247

Query: 247 MDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDI 306
            D               FS SFK  +  CLVKDPS+R SA  L  H F +  R  +DY  
Sbjct: 248 RDK-------------KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQAR-SSDY-- 307

Query: 307 VLLLKSIVNG-KDMVGGIQEMFRKNEDRL--KWLMDKKRTERSSIVNDSIGKTKSWEF 350
             + + +++G  D+V  +Q + RK ED L  + + D ++ E S   N+       W F
Sbjct: 308 --IARKLLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKEELSQ--NEYKRGISGWNF 339

BLAST of Lcy01g004450 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-39
Identity = 110/358 (30.73%), Postives = 176/358 (49.16%), Query Frame = 0

Query: 7   EFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNTIN 66
           ++P+  + Y +   IG     +V++A+C   +  VVA+K++D +  N   D ++      
Sbjct: 5   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDN--CDLNNISREAQ 64

Query: 67  SLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILRQL 126
           ++  ++H N+L  HC F V + +LW++MP++  GS   +  A + +G     +ATILR+ 
Sbjct: 65  TMMLVDHPNVLKSHCSF-VSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREA 124

Query: 127 LSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNVFNPIKGASD---------SVPYW 186
           L GL +LHQ GH+H+++K  NI L +   +KL D  V   +  + D           P W
Sbjct: 125 LKGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCW 184

Query: 187 EAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDW---MVLIAQFLKISRRFPYR 246
            APE+        Y FK+D+W+ G+  LELA G  PF+ +    VL+           Y 
Sbjct: 185 MAPEVMEQ--LHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYE 244

Query: 247 MDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYDI 306
            D               FS SFK  +  CLVKDPS+R SA  L  H F +  R  +DY  
Sbjct: 245 RDK-------------KFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQAR-SSDY-- 304

Query: 307 VLLLKSIVNG-KDMVGGIQEMFRKNEDRL--KWLMDKKRTERSSIVNDSIGKTKSWEF 350
             + + +++G  D+V  +Q + RK ED L  + + D ++ E S   N+       W F
Sbjct: 305 --IARKLLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKEELSQ--NEYKRGISGWNF 336

BLAST of Lcy01g004450 vs. TAIR 10
Match: AT1G23700.1 (Protein kinase superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 7.0e-39
Identity = 114/331 (34.44%), Postives = 175/331 (52.87%), Query Frame = 0

Query: 5   EDEFPMEAKAYEILCEIGVSEHTMVYKAICESMNSTVVAVKVVDMDGLNMGVDESHAKNT 64
           E  FP+ AK YEIL EIG      VY+A C  ++  +VA+K+ +++      D    +  
Sbjct: 6   ETRFPLVAKDYEILEEIGDG----VYRARCILLDE-IVAIKIWNLEKCTN--DLETIRKE 65

Query: 65  INSLSQINHANILSPHCCFTVDNRHLWIVMPFIHAGSLESVAAARFQNGLPGQFLATILR 124
           ++ LS I+H N+L  HC F +D+  LWIVMPF+  GS  ++  + + NGL    +A +LR
Sbjct: 66  VHRLSLIDHPNLLRVHCSF-IDSSSLWIVMPFMSCGSSLNIMKSVYPNGLEEPVIAILLR 125

Query: 125 QLLSGLVHLHQLGHVHKNIKPSNIFLDSEATIKLSDVNV----FNPIKGASDSV------ 184
           ++L  LV+LH LGH+H+N+K  N+ +DSE T+KL D  V    F+ ++    S       
Sbjct: 126 EILKALVYLHGLGHIHRNVKAGNVLVDSEGTVKLGDFEVSASMFDSVERMRTSSENTFVG 185

Query: 185 -PYWEAPELGRDSAADEYSFKSDVWAVGVVALELASGCLPFTDWMVLIAQFLKISRRFPY 244
            P   APE       D Y FK D+W+ G+ ALELA G  P T    ++   L+ S    Y
Sbjct: 186 NPRRMAPEKDMQQ-VDGYDFKVDIWSFGMTALELAHGHSPTT----VLPLNLQNSPFPNY 245

Query: 245 RMDLYNFVSKNAVDWRFSFSDSFKDFVGCCLVKDPSERASAASLSAHPFVELEREGTDYD 304
             D               FS SF++ V  CL++DP +R +A+ L  +PF++ +   T+Y 
Sbjct: 246 EEDT-------------KFSKSFRELVAACLIEDPEKRPTASQLLEYPFLQ-QTLSTEY- 302

Query: 305 IVLLLKSIVNGKDMVGGIQEMFRK-NEDRLK 324
              L  + ++G   +G   E +RK  E++ K
Sbjct: 306 ---LASTFLDGLSPLG---ERYRKLKEEKAK 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O233041.4e-5231.74Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P... [more]
Q551H41.1e-3130.13Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr... [more]
Q86IX16.0e-2729.33Serine/threonine-protein kinase dst1 OS=Dictyostelium discoideum OX=44689 GN=dst... [more]
G5EEN47.3e-2525.12Germinal center kinase 3 OS=Caenorhabditis elegans OX=6239 GN=gck-3 PE=1 SV=1[more]
P835101.6e-2427.48Traf2 and NCK-interacting protein kinase OS=Mus musculus OX=10090 GN=Tnik PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CA922.7e-9146.03serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1H6C51.1e-7639.83serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KYZ41.8e-7440.60serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3C8A91.1e-6035.77Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CN881.1e-6035.77serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028... [more]
Match NameE-valueIdentityDescription
XP_022138626.15.6e-9146.03serine/threonine-protein kinase BLUS1-like [Momordica charantia][more]
KAG7013499.11.8e-7640.38Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022958864.12.3e-7639.83serine/threonine-protein kinase BLUS1-like [Cucurbita moschata][more]
KAG6574930.12.3e-7639.83Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023548299.13.9e-7639.91serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G14480.11.0e-5331.74Protein kinase superfamily protein [more]
AT5G14720.13.0e-4231.03Protein kinase superfamily protein [more]
AT1G79640.11.4e-3930.73Protein kinase superfamily protein [more]
AT1G79640.21.4e-3930.73Protein kinase superfamily protein [more]
AT1G23700.17.0e-3934.44Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 4..96
e-value: 2.8E-16
score: 61.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 100..301
e-value: 1.1E-36
score: 128.0
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 34..162
e-value: 7.2E-7
score: 26.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..393
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 375..391
NoneNo IPR availablePANTHERPTHR48014SERINE/THREONINE-PROTEIN KINASE FRAY2coord: 4..471
NoneNo IPR availablePANTHERPTHR48014:SF7SERINE/THREONINE-PROTEIN KINASE BLUS1coord: 4..471
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 15..283
e-value: 4.7E-36
score: 124.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 15..283
score: 34.124435
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 12..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g004450.1Lcy01g004450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity