Lag0042122 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0042122
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr13: 36855888 .. 36857243 (-)
RNA-Seq ExpressionLag0042122
SyntenyLag0042122
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTGGACTAGTTGGAAAAATCTTTGTGTAGGGAAGGATGGAGGAGGCATGGGGTTTAGGGAGATAAGCCTCTTCAACCAAGCTATACTTGCTAAGCAAAGTTGGAGAATTTTAAGAAACCCGAATAGCCTCTTAGCCAAAACCCTTAGAGGTAGGTATTATAAGCATGAGGATTTCCTTAAAGCTCAATTAGGTCATAACCCTTCTTTAACCTGGAGGGGTATTGTTTGGGGTCGGGATCTTTTCACCAAGGGTTATAGATGGAGGGTTGGGTCGGGAAGTCGTATATACATTGATCAAGATCCATGGATCAATAGGCTAGGGTTGAGATACCCTGTCTATGTTCCTGAAGAGCTGAAAGGGAAGAATGTGAAGAGTATCATGACTGAGAACGACAAGTGGGACGAAGATAAGATAATGGAGAAGTTTTTTCCTCACGATGCAGAAGATATCCTTAAAATTCCCCTGGGTAATGGTAGAGGAAAGGATGAAATTATCTGGGCTCATAGCAAAAAGGGAATATTCACTGTTAAATCAGCATATCTCTTAGGCATGGATAACAAAGTTAGGGAGGAAACATCAGCTTCTGATAAGACCAAGTCTAAATCCCTTTGGAGGAAAGTTTGGAATCTTAATATCATCCCTAGAGCTAAAATATGTGTGTGGAAAATTCTCAAGAATCTTATCCCCACCAAAGCAAATCTCATATCAAAAGGAATCGACACGGATCATTTGTGTTTTCTTTGTAGGGCTAAGGAGGAATCATCTATTCATACTATGTGGGAGTGTAAGTTCTCTAAAAGCATTTGGAAAAGATTCTTTCCTAATGTAGCTAGCATCTTCGATTTGGGCAGGAGAAATTGGGAGGCGGTGGAGATTTGGGAGAAGATGACAGATCAAGCTACAGCAGAAGATACAAACAAAATGGCTATAATCCTTGGGAGCATTTGGAACTATCGTAACAGAGTCCTATCCAACGGAATAAAGCCAGATGCAGAGCAATTATCAAGATCAATTCAAACATCCTGGGAAGAGCATGTCAGAGTTAAAGAGAAAAACCTAGAAATCCTAAAATCGGAGAGCCACCCGAGTCAGGATTGTTGGACCCCCCCTTCCAATGCGTGCATGAAACTCAATGTTGATGCATCATGGAATAACTCGAGAGATATTGGAGGAGTAGGTTGGGTAGTTCGTGACTCCGAGGGATCTCTAATTATCGCCGGAGGAAAACCAATTAAAGAAAAATGGTTTGTGGAAGCGTTGGAATTGCTAGCGATCAAGGAAGGAATTGACAATTACCTCAAGATTTGCAGCGAGCGGCCAAAGCCCTTGACAGTGGAATCAGACTCGTAG

mRNA sequence

ATGCATTGGACTAGTTGGAAAAATCTTTGTGTAGGGAAGGATGGAGGAGGCATGGGGTTTAGGGAGATAAGCCTCTTCAACCAAGCTATACTTGCTAAGCAAAGTTGGAGAATTTTAAGAAACCCGAATAGCCTCTTAGCCAAAACCCTTAGAGGTAGGTATTATAAGCATGAGGATTTCCTTAAAGCTCAATTAGGTCATAACCCTTCTTTAACCTGGAGGGGTATTGTTTGGGGTCGGGATCTTTTCACCAAGGGTTATAGATGGAGGGTTGGGTCGGGAAGTCGTATATACATTGATCAAGATCCATGGATCAATAGGCTAGGGTTGAGATACCCTGTCTATGTTCCTGAAGAGCTGAAAGGGAAGAATGTGAAGAGTATCATGACTGAGAACGACAAGTGGGACGAAGATAAGATAATGGAGAAGTTTTTTCCTCACGATGCAGAAGATATCCTTAAAATTCCCCTGGGTAATGGTAGAGGAAAGGATGAAATTATCTGGGCTCATAGCAAAAAGGGAATATTCACTGTTAAATCAGCATATCTCTTAGGCATGGATAACAAAGTTAGGGAGGAAACATCAGCTTCTGATAAGACCAAGTCTAAATCCCTTTGGAGGAAAGTTTGGAATCTTAATATCATCCCTAGAGCTAAAATATGTGTGTGGAAAATTCTCAAGAATCTTATCCCCACCAAAGCAAATCTCATATCAAAAGGAATCGACACGGATCATTTGTGTTTTCTTTGTAGGGCTAAGGAGGAATCATCTATTCATACTATGTGGGAGTGTAAGTTCTCTAAAAGCATTTGGAAAAGATTCTTTCCTAATGTAGCTAGCATCTTCGATTTGGGCAGGAGAAATTGGGAGGCGGTGGAGATTTGGGAGAAGATGACAGATCAAGCTACAGCAGAAGATACAAACAAAATGGCTATAATCCTTGGGAGCATTTGGAACTATCGTAACAGAGTCCTATCCAACGGAATAAAGCCAGATGCAGAGCAATTATCAAGATCAATTCAAACATCCTGGGAAGAGCATGTCAGAGTTAAAGAGAAAAACCTAGAAATCCTAAAATCGGAGAGCCACCCGAGTCAGGATTGTTGGACCCCCCCTTCCAATGCGTGCATGAAACTCAATGTTGATGCATCATGGAATAACTCGAGAGATATTGGAGGAGTAGGTTGGGTAGTTCGTGACTCCGAGGGATCTCTAATTATCGCCGGAGGAAAACCAATTAAAGAAAAATGGTTTGTGGAAGCGTTGGAATTGCTAGCGATCAAGGAAGGAATTGACAATTACCTCAAGATTTGCAGCGAGCGGCCAAAGCCCTTGACAGTGGAATCAGACTCGTAG

Coding sequence (CDS)

ATGCATTGGACTAGTTGGAAAAATCTTTGTGTAGGGAAGGATGGAGGAGGCATGGGGTTTAGGGAGATAAGCCTCTTCAACCAAGCTATACTTGCTAAGCAAAGTTGGAGAATTTTAAGAAACCCGAATAGCCTCTTAGCCAAAACCCTTAGAGGTAGGTATTATAAGCATGAGGATTTCCTTAAAGCTCAATTAGGTCATAACCCTTCTTTAACCTGGAGGGGTATTGTTTGGGGTCGGGATCTTTTCACCAAGGGTTATAGATGGAGGGTTGGGTCGGGAAGTCGTATATACATTGATCAAGATCCATGGATCAATAGGCTAGGGTTGAGATACCCTGTCTATGTTCCTGAAGAGCTGAAAGGGAAGAATGTGAAGAGTATCATGACTGAGAACGACAAGTGGGACGAAGATAAGATAATGGAGAAGTTTTTTCCTCACGATGCAGAAGATATCCTTAAAATTCCCCTGGGTAATGGTAGAGGAAAGGATGAAATTATCTGGGCTCATAGCAAAAAGGGAATATTCACTGTTAAATCAGCATATCTCTTAGGCATGGATAACAAAGTTAGGGAGGAAACATCAGCTTCTGATAAGACCAAGTCTAAATCCCTTTGGAGGAAAGTTTGGAATCTTAATATCATCCCTAGAGCTAAAATATGTGTGTGGAAAATTCTCAAGAATCTTATCCCCACCAAAGCAAATCTCATATCAAAAGGAATCGACACGGATCATTTGTGTTTTCTTTGTAGGGCTAAGGAGGAATCATCTATTCATACTATGTGGGAGTGTAAGTTCTCTAAAAGCATTTGGAAAAGATTCTTTCCTAATGTAGCTAGCATCTTCGATTTGGGCAGGAGAAATTGGGAGGCGGTGGAGATTTGGGAGAAGATGACAGATCAAGCTACAGCAGAAGATACAAACAAAATGGCTATAATCCTTGGGAGCATTTGGAACTATCGTAACAGAGTCCTATCCAACGGAATAAAGCCAGATGCAGAGCAATTATCAAGATCAATTCAAACATCCTGGGAAGAGCATGTCAGAGTTAAAGAGAAAAACCTAGAAATCCTAAAATCGGAGAGCCACCCGAGTCAGGATTGTTGGACCCCCCCTTCCAATGCGTGCATGAAACTCAATGTTGATGCATCATGGAATAACTCGAGAGATATTGGAGGAGTAGGTTGGGTAGTTCGTGACTCCGAGGGATCTCTAATTATCGCCGGAGGAAAACCAATTAAAGAAAAATGGTTTGTGGAAGCGTTGGAATTGCTAGCGATCAAGGAAGGAATTGACAATTACCTCAAGATTTGCAGCGAGCGGCCAAAGCCCTTGACAGTGGAATCAGACTCGTAG

Protein sequence

MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDFLKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEELKGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKSAYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKMTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRVKEKNLEILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKEKWFVEALELLAIKEGIDNYLKICSERPKPLTVESDS
Homology
BLAST of Lag0042122 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-72
Identity = 147/431 (34.11%), Postives = 236/431 (54.76%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW  W+ +   K  GGMGFR++S FNQA++AKQ WRI++ P+SL+A+ L+ RY+KH  F
Sbjct: 635  IHWARWERISHSKARGGMGFRDLSSFNQALVAKQGWRIMQFPSSLVARVLKARYFKHTGF 694

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            + A LG  PS  WR IVWGR +  KG RWR+G+G  + +  + WI R     P+  P   
Sbjct: 695  MNAGLGSKPSFVWRSIVWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMG 754

Query: 121  KGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKS 180
                V  ++ E  +W ED I++ F P DAE I++IPL     +D++IW + KKG ++VKS
Sbjct: 755  TDTTVAELIDEKQQWREDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKS 814

Query: 181  AYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKG 240
             Y + M  K  E+ S S+    ++LWR +W L I  + KI +W+   +L+PT  NL  K 
Sbjct: 815  GYQVAMRIKFPEDPSCSN--HDQNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKK 874

Query: 241  IDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKMTD 300
            +  + +C  C    E+  H + EC  ++ IW+  + N+A     G    + V + +    
Sbjct: 875  VLQEPMCQSCHCHVETVSHALVECNRARKIWR--YSNLAEEL-RGVYRCDIVWMLQFWPR 934

Query: 301  QATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRVKEKNLEILKS 360
            Q    +  ++A +L +IW  RN+ L  G K +  ++  + +   E   ++++  +     
Sbjct: 935  QHAKVEGAEVAALLWAIWKARNKWLFEGKKENPLRVVANAEAIVESFKKIRQPEMVYKTK 994

Query: 361  ESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKEKWFVE 420
             +   Q  W+PP N   K+NVDA+ +    + G+G VVRDS+G+   A  K ++    V 
Sbjct: 995  GNAERQKQWSPPPNGWQKVNVDAAVDVENQMAGLGVVVRDSDGNCRAAAIKSLRLPGSVA 1054

Query: 421  ALELLAIKEGI 432
              E  A++ G+
Sbjct: 1055 MAEATAMEWGL 1060

BLAST of Lag0042122 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 281.2 bits (718), Expect = 1.6e-71
Identity = 162/459 (35.29%), Postives = 249/459 (54.25%), Query Frame = 0

Query: 3    WTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDFLK 62
            W SWK LC  K  GGMGFR +  FN A+LAKQ WR++ NPNSL+A+  + RYY H D  +
Sbjct: 678  WVSWKKLCKAKKNGGMGFRNLQAFNLAMLAKQGWRLISNPNSLVAQIYKARYYPHGDVFQ 737

Query: 63   AQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEELKG 122
            A+LG +PS TWR I  G ++  +G RWRVG+G RI I +D W+    + Y V  P +   
Sbjct: 738  AKLGASPSYTWRSIFNGLEVVRRGTRWRVGNGERILIWEDKWL-PTPITYKVISPPKPFD 797

Query: 123  KNVK---SIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVK 182
               +    I  E  +W +D + + F P +A  IL IPL +   +D+IIW  ++KG F+VK
Sbjct: 798  DYPRVSALIDRERKRWKDDVVRDLFLPFEARTILSIPLSHNFPEDQIIWVGNRKGEFSVK 857

Query: 183  SAYLLG---MDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANL 242
            SAY +    +DN    E+S+ D      LWRK+W+LNI P+ +I  WK+  N +PT  NL
Sbjct: 858  SAYYIAVGVIDNLEVGESSSGD--SRSLLWRKLWHLNIPPKVRIFAWKMCMNALPTFLNL 917

Query: 243  ISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWE 302
            + KG++   +C  C  + ES+IH   +C+ +K +W+ +  N A   DL   N + V+I  
Sbjct: 918  LRKGVNICDVCPACGMEPESNIHIFVKCEVAKRVWRCWLDNPA---DLVNVNMDIVDIAM 977

Query: 303  KMTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVR--VKEKN 362
            K+ D  T  D     ++  +IW  RN+++        E LS+  +  W   ++  ++ KN
Sbjct: 978  KILDFGTPSDLEIFFVVAWAIWCNRNKIV-------FESLSQVPEFIWGFAIKYILEFKN 1037

Query: 363  LEILKSESHPSQD-CWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPI 422
                  +  P  D  W  P     K+NVD + +       VG ++RD+ G +  A    +
Sbjct: 1038 ASATYCQILPQSDGKWMAPPPGVFKINVDGATSEYGRNSSVGVIIRDATGDVCAACCTYL 1097

Query: 423  KEKWFVEALELLAIKEGIDNYLKICSERPKP-LTVESDS 452
            + ++ VE +E LA++ G    L +  E+  P + +ESD+
Sbjct: 1098 QGQYSVEEVEALAMERG----LLLAKEQKLPQIIMESDA 1119

BLAST of Lag0042122 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 280.4 bits (716), Expect = 2.7e-71
Identity = 150/451 (33.26%), Postives = 243/451 (53.88%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW  W ++   K  GG+GFR++  FNQA++AKQ WR++R PNSL+A+ ++ RYYK+  F
Sbjct: 930  IHWARWDSMSKAKRRGGLGFRDLPSFNQALVAKQGWRLVRYPNSLMARVMKARYYKNSTF 989

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
              A++G NPS  WR I+WG  +  KG RWR+G G ++ + +D WI R     P+  P+ L
Sbjct: 990  WNAKVGSNPSFIWRSILWGSQVIKKGVRWRIGDGKKVLVYKDKWIPRPATFQPI-SPKTL 1049

Query: 121  KGKN-VKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVK 180
              +  V  ++   +KW  D++ + F   D E ILKI L +G+ +DE++W   KKG ++VK
Sbjct: 1050 PHETVVADLIDSENKWRVDRLEQHFMKEDIEAILKILLPSGKEEDEVLWHFDKKGEYSVK 1109

Query: 181  SAYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISK 240
            S Y L ++     E  +S+   S  LW+  W L++  + KI +W+ LKN++PT  NL  +
Sbjct: 1110 SGYQLALNQNFPNEPESSN--SSSRLWKIPWMLDLPEKVKIFMWRALKNILPTAENLWKR 1169

Query: 241  GIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKMT 300
                + +C  C+ + E+  H + ECK ++ IW      V    D  +  + A+   ++M 
Sbjct: 1170 RSLQEPICQRCKLQVETVSHVLIECKAARKIWDLAPLIVQPSKDHNQDFFSAI---QEMW 1229

Query: 301  DQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRVKEKNLEILK 360
             +++  +   M +    IW+ RN+ +  G K D+  L+    +  + + RV +       
Sbjct: 1230 SRSSTAEAELMIVYCWVIWSARNKFIFEGKKSDSRFLAAKADSVLKAYQRVSKPGNVHGA 1289

Query: 361  SESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKEKWFV 420
             +    Q  W PPS   +KLNVDA+ +      G+G +VRD+EG ++  G K  + +  V
Sbjct: 1290 KDRGIDQQKWKPPSQNVLKLNVDAAVSTKDQKVGLGAIVRDAEGKILAVGIKQAQFRERV 1349

Query: 421  EALELLAIKEGIDNYLKICSERPKPLTVESD 451
               E  AI  G+    +I S     L VESD
Sbjct: 1350 SLAEAEAIHWGLQVANQISS---SSLIVESD 1371

BLAST of Lag0042122 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 7.4e-69
Identity = 134/436 (30.73%), Postives = 233/436 (53.44%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW +W +L + K  GGMGFR++ LFN+A+LAKQ WRIL +PNS+L++ L+GRY+K   F
Sbjct: 629  IHWVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSF 688

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            ++A++  NPS  WR I+WGRDL  KG RWR+G+G  ++I  D W+        +  P   
Sbjct: 689  MEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLP 748

Query: 121  KGKNVKSIMT-ENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVK 180
                V S++  E   W  D + ++F P +A+ IL IP+G G  +D +IW + K G+++V+
Sbjct: 749  LVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVR 808

Query: 181  SAYLLG-MDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLIS 240
            S Y +  ++N   +  S+S   + +  W   W ++I  + K+ +W++  + +PT  NL  
Sbjct: 809  SGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSK 868

Query: 241  KGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKM 300
            +G++  + C+ C    E SIH  W CKF++++W        S F + R + E++      
Sbjct: 869  RGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESL------ 928

Query: 301  TDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRV---KEKNL 360
                +  D  ++ +++  +WN RN    N       ++   +   W     +   + K+ 
Sbjct: 929  ----SKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMEL-VEWANKYAMEFREAKSN 988

Query: 361  EILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKE 420
             I    ++ ++  W PP     K+N DAS+  S    G+G ++ +  G ++ A  K ++ 
Sbjct: 989  PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLEN 1048

Query: 421  KWFVEALELLAIKEGI 432
               V+  E +A  EG+
Sbjct: 1049 IQSVDMAEAIAAVEGL 1053

BLAST of Lag0042122 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 269.6 bits (688), Expect = 4.8e-68
Identity = 137/415 (33.01%), Postives = 227/415 (54.70%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW  W+ L   K  GG+GFRE S FNQA++AKQ+WR+L+ PNSL+++ L+ RY+++  F
Sbjct: 871  IHWRKWEKLSQAKIRGGLGFREFSCFNQALVAKQAWRLLQYPNSLVSRVLQARYFRNSSF 930

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            L A+ G N S  WR I+WGR +  KG RWR+G+G +I I  D W+ R     P++     
Sbjct: 931  LCAKAGANASYIWRSIMWGRQVIKKGMRWRIGNGKKIAIFSDNWLPRPETFRPIFPLSLP 990

Query: 121  KGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKS 180
                V  ++  +++WDE K+ + F   D  +ILKIPL   + +DE++W + K+G ++VKS
Sbjct: 991  VSSVVADLIKADNQWDEIKLRQHFLDVDTAEILKIPLPAEKAEDEVLWHYDKRGNYSVKS 1050

Query: 181  AYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKG 240
             Y L + +K  + TS ++   S   W  +W L +  + KI +W+   NL+P+  NL  + 
Sbjct: 1051 GYQLALRSKFPDSTSCTE--ASHKYWSALWTLELPEKLKIFMWRASNNLLPSAENLWKRK 1110

Query: 241  IDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIW---EK 300
            +  +  C  C+   E+  H + ECK ++ IW      + S F   R    + +I+   + 
Sbjct: 1111 VVEEPTCKRCKLSVETISHALLECKAARKIW------LQSPFSAPRLEANSQDIFSTLQN 1170

Query: 301  MTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPD---AEQLSRSIQTSWEEHVRVKEKN 360
            M  +    D   M  +  S W  RN+ + +G + +   +   + S+ T+++   + ++ +
Sbjct: 1171 MAKELRKSDLELMVALCWSAWYARNKCIFDGRELNPIISAAKAESVLTAFQRVRKPQQSH 1230

Query: 361  LEILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAG 410
            + I   E    Q  W PP     K+NVDA++N+     GVG V+RDS G ++ AG
Sbjct: 1231 ISISIKE---KQQEWLPPPQNVFKVNVDAAFNSKNLSAGVGAVIRDSNGKIVAAG 1274

BLAST of Lag0042122 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 5.8e-24
Identity = 98/419 (23.39%), Postives = 170/419 (40.57%), Query Frame = 0

Query: 2   HWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHE--D 61
           H   W  +C  K  GG+G R     N+A+++K  WR+L+  NSL    L+ +Y+  E  D
Sbjct: 85  HLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGEIRD 144

Query: 62  FLKAQLGHNPSLTWRGIVWG-RDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRY--PVYV 121
                   + S TWR I  G RD+ + G  W  G G +I    D W++   L        
Sbjct: 145 SRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKPLLELDNGER 204

Query: 122 PEELKGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRG-KDEIIWAHSKKGI 181
           P +      K +      WD  KI      +   ++  + L    G +D + W  S+ G 
Sbjct: 205 PTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTGARDRLSWKFSQDGQ 264

Query: 182 FTVKSAY-LLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKA 241
           F+V+SAY +L +D   R   +        S +  +W + +  R K  +W +    + T+ 
Sbjct: 265 FSVRSAYEMLTVDEVPRPNMA--------SFFNCLWKVRVPERVKTFLWLVGNQAVMTEE 324

Query: 242 NLISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFP--NVASIFDLGRRNWEAV 301
               + +   ++C +C+   ES +H + +C     IW R  P       F      W   
Sbjct: 325 ERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRRQQGFFSKSLFEW--- 384

Query: 302 EIWEKMTDQATAED---TNKMAIILGSIWNYR-NRVLSNGIKPDAEQLSRSIQTSWEEHV 361
            +++ + D++  ED   +   A+I+   W +R   +     K       + ++    E  
Sbjct: 385 -LYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFGENTK--CRDRVKFVKEWAVEVY 444

Query: 362 RVKEKNLEILKSESHPSQD---CWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGS 405
           R    N  +L   + P  +    W  P    +K+N D +   +  +   G V+RD  G+
Sbjct: 445 RAHSGN--VLVGITQPRVERMIGWVSPCVGWVKVNTDGASRGNPGLASAGGVLRDCTGA 487

BLAST of Lag0042122 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 4.1e-22
Identity = 45/104 (43.27%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 3   WTSWKNLCVGK-DGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDFL 62
           W +W+ LC  K D GG+GFR++  FNQA+LAKQS+RI+  P++LL++ LR RY+ H   +
Sbjct: 41  WVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMM 100

Query: 63  KAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWI 106
           +  +G  PS  WR I+ GR+L ++G    +G G    +  D WI
Sbjct: 101 ECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lag0042122 vs. ExPASy TrEMBL
Match: A0A1S8ACU2 (Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-70
Identity = 144/450 (32.00%), Postives = 232/450 (51.56%), Query Frame = 0

Query: 2   HWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDFL 61
           HW SW+ LC  K  GG+GFR+I+ FNQA++AKQSWRI++ P SL+AK L+ +Y+K  DFL
Sbjct: 82  HWASWEKLCQAKGRGGLGFRDITSFNQALVAKQSWRIIKYPESLMAKILQAKYFKGADFL 141

Query: 62  KAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEELK 121
           +A+LG  PS  WR I+WGR +   G RWR+G+G R+ I +  WI R     P+  P    
Sbjct: 142 QAKLGSKPSFVWRSIIWGRQVIVNGMRWRIGTGDRVKIYKSHWIPRPQAFKPISAPTLGM 201

Query: 122 GKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKSA 181
              V  ++ EN KW E  I + F   DAE I +I L      D+I+W + KKG ++VKS 
Sbjct: 202 DCTVAELIDENQKWKESLIQQHFNFEDAELISRIQLPISPKPDQILWHYDKKGNYSVKSG 261

Query: 182 YLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKGI 241
           Y + M  +++     S  + +   W  +W+L +  + KI +WK  +N +PT  NL  + +
Sbjct: 262 YQIAM--RIKHPARPSSSSSNLGQWNVIWSLELPEKIKIFMWKAARNFLPTSENLWRRKM 321

Query: 242 DTDHLCFLCRAKEESSIHTMWECKFSKSIWKRF-FPNVASIFDLGRRNWEAVEIWEKMTD 301
             + +C  C+ K+E   H +  CK +K +WK   F     + D    N + + + +++ +
Sbjct: 322 VQEPICPRCKMKKEDINHAIMVCKAAKKMWKLTPFVEEMQLLD----NQDLLSMLQELVN 381

Query: 302 QATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRVKEKNLEILKS 361
           + + ++   +  +  + W+ RN  +    + D +      +   E + RV+   ++    
Sbjct: 382 RRSKDELRLIIALCWTAWHTRNIFVFENKRQDPQISVAKAEAVVESYARVRMTKVQAAAK 441

Query: 362 ESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKEKWFVE 421
                   W PP     K NVDA+ N  ++  G+G V+RD  G++IIA     K    V 
Sbjct: 442 AIPAKGAKWIPPPQGHFKANVDAAVNKEKNQVGLGVVIRDDSGAIIIAAVNHTKYHGDVA 501

Query: 422 ALELLAIKEGIDNYLKICSERPKPLTVESD 451
             E  A+  G+     +      PL +E+D
Sbjct: 502 QAEAAAVNFGLQ---VVMEASLSPLILETD 522

BLAST of Lag0042122 vs. ExPASy TrEMBL
Match: A0A803Q6Y1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 1.9e-70
Identity = 147/449 (32.74%), Postives = 235/449 (52.34%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW SWK LC  KD GGMGFR    FNQA+LAKQ+WR+   P+SLL K L+ RYY +  F
Sbjct: 2533 IHWRSWKLLCKRKDEGGMGFRSFVHFNQALLAKQAWRLHSQPDSLLTKLLKCRYYSNNSF 2592

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            L+A LGH+PSLTW+GI WG+ L   G RW++  G RI+  QDPWI       P +    +
Sbjct: 2593 LEASLGHSPSLTWQGIHWGKKLLISGLRWKIREGCRIHCRQDPWIPGHSTFLPFHYNGPI 2652

Query: 121  KGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKS 180
             G  V +++ +N +WD D + + FF  D E IL IPL   R  D +IW H   G++TV++
Sbjct: 2653 NGV-VSNLIKDNREWDLDLLQQWFFQPDVERILTIPLSFFRNNDSLIWHHHTSGLYTVQT 2712

Query: 181  AYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKG 240
             Y L   + + +    S    +   W+  W L +  + KI  W++  + +P    L+ + 
Sbjct: 2713 GYHLA--SSLEDADFCSGSYSNSPWWKFFWALQLPQKIKIFTWRVFHDALPVATGLVKRK 2772

Query: 241  IDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEK--- 300
            + TD  C +CR   E+  H ++ CK++K++W+       S F+    +W      +K   
Sbjct: 2773 VITDSTCSICRQAWETVGHALFGCKYAKAVWR------ISGFNF---DWYKASSMKKGDY 2832

Query: 301  ---MTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSR-------SIQTSWEEH 360
               ++   +  +  ++  +L SIW  RNRV+       A +LS        + + +  ++
Sbjct: 2833 LVHLSSIHSKSEMERIICMLWSIWTERNRVVHGHKAKQARELSTFAENYLLNYRAALSKY 2892

Query: 361  VRVKEKNLEILKSESHPS-----QDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSE 420
             +  +K +  + + +HPS        W PP  A  KLN+DA+ + +    G+G ++RDS 
Sbjct: 2893 QQPLQKPVAAIGNGTHPSTSNGHNPPWKPPPPAIFKLNIDAANDRTTHTTGLGAIIRDST 2952

Query: 421  GSLIIAGGKPIKEKWFVEALELLAIKEGI 432
            G ++ A  K +   +    +E LA+  G+
Sbjct: 2953 GFIVAAFSKKLLGSFDPHEMEALALFHGV 2969

BLAST of Lag0042122 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 3.6e-69
Identity = 134/436 (30.73%), Postives = 233/436 (53.44%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW +W +L + K  GGMGFR++ LFN+A+LAKQ WRIL +PNS+L++ L+GRY+K   F
Sbjct: 629  IHWVAWNSLYLPKCEGGMGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSF 688

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            ++A++  NPS  WR I+WGRDL  KG RWR+G+G  ++I  D W+        +  P   
Sbjct: 689  MEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLP 748

Query: 121  KGKNVKSIMT-ENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVK 180
                V S++  E   W  D + ++F P +A+ IL IP+G G  +D +IW + K G+++V+
Sbjct: 749  LVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVR 808

Query: 181  SAYLLG-MDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLIS 240
            S Y +  ++N   +  S+S   + +  W   W ++I  + K+ +W++  + +PT  NL  
Sbjct: 809  SGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSK 868

Query: 241  KGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKM 300
            +G++  + C+ C    E SIH  W CKF++++W        S F + R + E++      
Sbjct: 869  RGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKLSPFLILRESHESL------ 928

Query: 301  TDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRV---KEKNL 360
                +  D  ++ +++  +WN RN    N       ++   +   W     +   + K+ 
Sbjct: 929  ----SKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMEL-VEWANKYAMEFREAKSN 988

Query: 361  EILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKPIKE 420
             I    ++ ++  W PP     K+N DAS+  S    G+G ++ +  G ++ A  K ++ 
Sbjct: 989  PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLEN 1048

Query: 421  KWFVEALELLAIKEGI 432
               V+  E +A  EG+
Sbjct: 1049 IQSVDMAEAIAAVEGL 1053

BLAST of Lag0042122 vs. ExPASy TrEMBL
Match: A0A803QEG9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 4.7e-69
Identity = 152/471 (32.27%), Postives = 245/471 (52.02%), Query Frame = 0

Query: 1    MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
            +HW SW  LC  K  GGMGFR    FNQA+LAKQ+WRI+  PNSLL+  L+ +Y+ H  F
Sbjct: 1346 IHWRSWNLLCKTKGEGGMGFRSFIQFNQALLAKQAWRIVEYPNSLLSTLLKSKYFPHNSF 1405

Query: 61   LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
            L+A +GH+PSLTW+GI WGR+L   G RW++G G  +    DPW+       P+      
Sbjct: 1406 LEASMGHSPSLTWQGIRWGRELLAHGLRWKIGEGRTVRSGFDPWLPGHSSFLPITYSGPP 1465

Query: 121  KGKNVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVKS 180
             G  V +++T+  +W+   + + F P D E IL +PL     +D++IW H   G FTV+S
Sbjct: 1466 NGV-VANLITDERQWNIPLLQQYFCPLDIEKILTLPLSFFPTRDKLIWHHHSSGNFTVQS 1525

Query: 181  AYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISKG 240
            AY L     +  E  +S  T +   W+  W+L I  + KI  W+++ + +P   +L+ + 
Sbjct: 1526 AYHLA--TSLENEDLSSSSTSAVQWWKHFWSLQIPQKVKIFAWRVIHDALPVATSLVRRK 1585

Query: 241  IDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGRRNWEAVEIWEKMTD 300
            I TD  C +C+   ES+ H ++ CK++K++W+ F  N      +  +N + +     + +
Sbjct: 1586 IITDSTCSICKQAWESTGHALFGCKYAKAVWRSFHFNFNWSAAISMKNGDYLMYLSSIYN 1645

Query: 301  QATAEDTNKMAIILGSIWNYRNRVL-------SNGIKPDAEQLSRSIQTSWEEHVRVKEK 360
            +A  E+   +  I+ +IW  RN+V+       +N +   A    ++ + +  +H  V   
Sbjct: 1646 KAEMEE---LFCIMWAIWGERNKVVHGKNARRANDLAVFATVFLQNFRAA--QHKPVAAA 1705

Query: 361  NLEILKSESHPSQ------------DCWTPPSNACMKLNVDASWNNSRDIGGVGWVVRDS 420
                    + PSQ              W PP+  C KLN DA+  NS +  GVG V+RD+
Sbjct: 1706 TAPAATDVAGPSQQPSKPPAATAALSSWRPPAADCYKLNTDAAAENSSNTIGVGAVLRDA 1765

Query: 421  EGSLIIAGGKPIKEKWFVEALELLAIKEGIDNYLKICSERPKPLT-VESDS 452
             GS+  A   PI   +    +E  A+   ++  L    ++  P+T +E+D+
Sbjct: 1766 SGSVTGALAMPIIGNFKSHEMEAKALFHSLNWAL----QQQLPITHIETDA 1804

BLAST of Lag0042122 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.0e-68
Identity = 154/458 (33.62%), Postives = 240/458 (52.40%), Query Frame = 0

Query: 1   MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
           +HW  W+ LC  KD GG+GFR++ +FNQA+LAKQ WR LR+P  L ++ L+  Y+  +  
Sbjct: 183 IHWCKWRYLCRPKDKGGLGFRDLGMFNQALLAKQIWRCLRHPQLLCSRVLKASYFPRKGV 242

Query: 61  LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLGLRYPVYVPEEL 120
           L+A  G N S  WR +VWG+ L  KGYRWRVG+G  + + +DPW+ R  + + VY    L
Sbjct: 243 LEAGCGANASFVWRSLVWGKKLILKGYRWRVGNGESVRVLEDPWLPR-PVTFKVYDQPSL 302

Query: 121 KGK-NVKSIMTENDKWDEDKIMEKFFPHDAEDILKIPLGNGRGKDEIIWAHSKKGIFTVK 180
                V  +   + +WDE  I   F P D + IL IP  +   +D+I+W +SK G ++VK
Sbjct: 303 PANLYVTDLKLADGQWDEGFIRSIFNPTDVDLILGIPCSDWDFEDKILWHYSKYGEYSVK 362

Query: 181 SAYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILKNLIPTKANLISK 240
           S Y   M      E   S++      W+K+W L I P+ K  VWK+  N +P   NL  +
Sbjct: 363 SGY--RMAASFTTEQHQSNEHSIVQWWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKR 422

Query: 241 GIDTDHLCFLCRAK-EESSIHTMWECKFSKSIWKRFFPNVASIFD-----LGRRNWEAVE 300
           GI +  +C  C +  +ES  H +WECK SK  W+     V+ ++D     LG  N   + 
Sbjct: 423 GIASSVVCSRCSSHVDESVAHALWECKASKGYWR-----VSGLYDDLKQMLGEDN---LT 482

Query: 301 IWEKMTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDAEQLSRSIQTSWEEHVRVKEK 360
           +  ++  +   E      ++  +IWN RN V+  G  P  E++       W  +     +
Sbjct: 483 MLMRIAAEWDKEKLEFFLLVSWNIWNVRNTVVHGGYHPKPEEM-----IEWCGNFLADFR 542

Query: 361 NLEILKSESHPSQDC-WTPPSNACMKLNVDASWNNSRDIGGVGWVVRDSEGSLIIAGGKP 420
                +     S+D  W PP+   + +NVDA       I G+G+VVRD+ G ++ A    
Sbjct: 543 GDTGRERSQRSSEDSRWVPPARDQVTINVDAGVKQGGLISGLGYVVRDAAGVVLSAAATV 602

Query: 421 IKEKWFVEALELLAIKEGIDNYLKICSERPKPLTVESD 451
           ++++     LEL+AIK+GI   ++   +R +  +VE+D
Sbjct: 603 LQQELPPLQLELMAIKKGIQVGIQ---QRLQRFSVETD 621

BLAST of Lag0042122 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 3.3e-51
Identity = 126/456 (27.63%), Postives = 215/456 (47.15%), Query Frame = 0

Query: 1   MHWTSWKNLCVGKDGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDF 60
           MHW +W +L   K  GG+GF++I  FN A+L KQ WR+L  P SL+AK  + RY+   D 
Sbjct: 39  MHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDP 98

Query: 61  LKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWIN----RLGLRYPVYV 120
           L A LG  PS  W+ I   +++  +G R  VG+G  I I +  W++       LR     
Sbjct: 99  LNAPLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVP 158

Query: 121 PEELKGKNVKSIMTENDKWDED------KIMEKFFPHDAEDIL-KIPLGNGRGKDEIIWA 180
           P+E    +V SI+  +D  DE        ++E  FP     ++ ++  G  R  D   W 
Sbjct: 159 PQEY--ASVSSILKVSDLIDESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWD 218

Query: 181 HSKKGIFTVKSAY--LLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKILK 240
           ++  G +TVKS Y  L  + NK R       +     +++K+W     P+ +  +WK L 
Sbjct: 219 YTSSGDYTVKSGYWVLTQIINK-RSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLS 278

Query: 241 NLIPTKANLISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIW--------------KR 300
           N +P    L  + +  +  C  C + +E+  H +++C F++  W                
Sbjct: 279 NSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSIPIPLGGEWADS 338

Query: 301 FFPNVASIFDLGRRNWEAVEIWEKMTDQATAEDTNKMAIILGSIWNYRNRVLSNGIKPDA 360
            + N+  +F+LG  N +    WEK         +  +  +L  +W  RN ++  G + +A
Sbjct: 339 IYVNLYWVFNLGNGNPQ----WEKA--------SQLVPWLLWRLWKNRNELVFRGREFNA 398

Query: 361 EQLSRSIQTSWEE-HVRVKEKNLEILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIG 420
           +++ R  +   EE  +R + ++       +  S   W PP +  +K N DA+WN   +  
Sbjct: 399 QEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGRWRPPPHQWVKCNTDATWNRDNERC 458

Query: 421 GVGWVVRDSEGSLIIAGGKPIKEKWFVEALELLAIK 429
           G+GWV+R+ +G +   G + + +   V   EL A++
Sbjct: 459 GIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

BLAST of Lag0042122 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-27
Identity = 90/372 (24.19%), Postives = 156/372 (41.94%), Query Frame = 0

Query: 50  LRGRYYKHEDFLKAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWINRLG 109
           ++ RY+K    L A++    S  W  ++ G  L  KG R  +G G  I I  D  ++   
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

Query: 110 LRYPVYVPEELKGKNVKSIMTENDK---WDEDKIMEKFFPHDAEDILKIPLGNGRGKDEI 169
            R P+   E  K   + ++         WD+ KI +     D   I +I L   +  D+I
Sbjct: 61  PR-PLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKI 120

Query: 170 IWAHSKKGIFTVKSAYLLGMDNKVREETSASDKTKSKSLWRKVWNLNIIPRAKICVWKIL 229
           IW ++  G +TV+S Y L   +      + +    S  L  ++WNL I+P+ K  +W+ L
Sbjct: 121 IWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRAL 180

Query: 230 KNLIPTKANLISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASIFDLGR 289
              + T   L ++G+  D  C  C  + ES  H ++ C F+   W+    ++     +  
Sbjct: 181 SQALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSN 240

Query: 290 RNWEAVEIWEKMTDQATAEDTNKM--AIILGSIWNYRNRVLSNGIK--PDAEQLSRSIQT 349
              E +          T  D +K+    ++  IW  RN V+ N  +  P    LS   +T
Sbjct: 241 DFEENISNILNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAET 300

Query: 350 -SW----EEHVRVKEKNLEILKSESHPSQDCWTPPSNACMKLNVDASWNNSRDIGGVGWV 409
             W    + H +      +I +++       W  P    +K N DA ++  +     GW+
Sbjct: 301 HDWLNATQSHKKTPSPTRQIAENKIE-----WRNPPATYVKCNFDAGFDVQKLEATGGWI 360

BLAST of Lag0042122 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 107.5 bits (267), Expect = 2.9e-23
Identity = 45/104 (43.27%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 3   WTSWKNLCVGK-DGGGMGFREISLFNQAILAKQSWRILRNPNSLLAKTLRGRYYKHEDFL 62
           W +W+ LC  K D GG+GFR++  FNQA+LAKQS+RI+  P++LL++ LR RY+ H   +
Sbjct: 41  WVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMM 100

Query: 63  KAQLGHNPSLTWRGIVWGRDLFTKGYRWRVGSGSRIYIDQDPWI 106
           +  +G  PS  WR I+ GR+L ++G    +G G    +  D WI
Sbjct: 101 ECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lag0042122 vs. TAIR 10
Match: AT4G10613.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 54.7 bits (130), Expect = 2.3e-07
Identity = 31/108 (28.70%), Postives = 52/108 (48.15%), Query Frame = 0

Query: 222 VWKILKNLIPTKANLISKGIDTDHLCFLCRAKEESSIHTMWECKFSKSIWKRFFPNVASI 281
           +W    + +PT+A L+S G+    LC LC    E+  H +  C FS SIW      +  +
Sbjct: 1   MWISNLDRLPTRARLVSWGLQISPLCCLCSQSVETRDHLILTCGFSSSIWNMVQARL-RL 60

Query: 282 FDLGRRNWEAVEIWEKMTDQATAEDTNKMA--IILGSIWNYRNRVLSN 328
             +  R WE++  W K++  ++     K+     + +IW  RN +L N
Sbjct: 61  QPVQFRVWESLLSWIKLSTASSPSIIRKLVAQATVCAIWKQRNNLLHN 107

BLAST of Lag0042122 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-06
Identity = 21/60 (35.00%), Postives = 35/60 (58.33%), Query Frame = 0

Query: 209 VWNLNIIPRAKICVWKILKNLIPTKANLISKGIDTDHLCFLCRAKEESSIHTMWECKFSK 268
           +W+L I P+ K+ +WK L N +P  A L+S+ I  +  C  CR   E+  H ++ C F++
Sbjct: 9   IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCR-DFETITHILFNCPFAQ 67

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_024037590.11.4e-7234.11uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_023881891.11.6e-7135.29uncharacterized protein LOC111994244 [Quercus suber][more]
XP_006491472.12.7e-7133.26uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_022150918.17.4e-6930.73uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_024950112.14.8e-6833.01uncharacterized protein LOC112496847 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
P0C2F65.8e-2423.39Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P932954.1e-2243.27Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A1S8ACU21.5e-7032.00Ribonuclease H-like superfamily protein OS=Citrus limon OX=2708 PE=4 SV=1[more]
A0A803Q6Y11.9e-7032.74Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DAR43.6e-6930.73uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803QEG94.7e-6932.27Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QQT21.0e-6833.62Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.13.3e-5127.63Ribonuclease H-like superfamily protein [more]
AT3G09510.12.6e-2724.19Ribonuclease H-like superfamily protein [more]
ATMG00310.12.9e-2343.27RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G10613.12.3e-0728.70RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G26855.12.5e-0635.00RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 380..451
e-value: 5.3E-7
score: 29.5
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 176..271
e-value: 4.8E-17
score: 62.4
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 64..408
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 64..408
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 379..451
e-value: 3.5191E-9
score: 52.7016

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0042122.1Lag0042122.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity