Lag0042064 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0042064
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDUF4283 domain-containing protein
Locationchr13: 35797538 .. 35799212 (+)
RNA-Seq ExpressionLag0042064
SyntenyLag0042064
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGGAAAGATCACTCGTTGTTTCTCGATTGATGGCTCATTATTCTTGGAAGAAAGTTCATATTGCCCTTGAGAATTTCTTTAAATCCTCTGTCTCGATTAACCCCTTCATGGATGATAAAGCTTTGGTTCATGTTGTAGTTGGTGGCCTGGAATTTTCAGCAATTGGTAAGTGGAAGAGATTTGGGGACTTACACTTGAAAATGGAGTTTTGGTCCTTTGATATTCATTCACAGCCGAAGGTTATAAAAAGTTATGGAGGGTGGATAGCTATTAGAAATCTTCCACTGGATTTATGGCATCGTGATTCCTTTGAGGCTATTGGAAAGAATCTTGGAGGGTTGGTAAGTATATTTTCCAATACTCTTAATTTGTTAGATTGTTCTAAAGCCTTTATTGAAGTTGAAAAGAATTTTTGTGGATTTATTCCTGCTGATATTAATGTTAAGATTGGTAGCAAGTATGAATTTTCATTAAGATATGGTGATATTAATTCTTTAGAAACCAGAAATCTGAATCTTGATTCAAGAAAAATGCTCAATGTCAATGACTTTTCAAATTCCCTGGATTCAAGTAGGGTTAGGAAAGTAATTTTGGATGAAGATTCTGATAACAAAAATGATGGGGAAGGGATGAGTGAAAGCCATCTAAATCTCGGTATCAGGAGGAATTTAATGAGGAATTGGTTCCTTCAAAGGTTGTCTCGGTACAAGAAAAATGTATTAATTGCAGCAGCTGTAATATCCCTTCAACCAAGATGATTAATGCTATTGATAGCTGTTTTTTGAATAATGAAGTACAACAGGTTTGAAAAGAGGATGAATCATCAGCATTAATTTCTCATTCTTCTGCCCCTTCAATTCCAGAGCCTTCATCCGAAAAGTTAGCATTGAATAGGGAGTCTTCATTGGCATTAAATCTGGAGCATTCTTCTGAAGTTATCCCTATTTTGTCTCCAATTTTAAATAATCACTCTTTCAATCATTTGTTGCCTGATGTTCCCAAGACGACTAAGGAAATTCTTCCTCCTTTATTCGGTGACTCTTCAAGTCCACAAAGCATTCAAGATGTCAAAAATAAATTCATTCAGACCTATTCTCATTTTTATACTCGGAAGAAGAAAGAAGTTGCTTCATCATCTATTGCTAAGGATTCTCACGGTAATCCAGATGCTATTGAAGAGGCCTGTTCTTTATTATTGGCTCCTTCTCTTAAGCAAGCTTCGTATTCTGGGAAATCATTAATGTTCATCCACAATAATTGTCTTTTAATAATTGCAGCCTTACTAAAATTCCAATTGGGGAATCCAGAGATGTCTTCATCAAGGGTATTTTTTCTCATGATCAAAATGTTTCTATTAATTGTCCAATTTCTATCTCTTGTATGGAAGAATCTGATGTGGCTTCTGATGTCAGCTTAAGTAGTGAAGAATTTGAACCACATTCTATGAGTCAACCTAATGATACCATTATTGAAGACACTATTGAAGACACTTATGCTGAAACTTTGAGATTATTATTTCAGGATGATGAGCAGAACTTTGATCCTCCTAATTTAAATGTTTCTTCTCAATCACCTATAGCTATTCCGGATAATTTCTCTTCGTTGGTTGATGCGTGTGGTTTCCAGATTAAAGAAATTCCTCCTTTAAATCCAAAGACAATTCAGTAA

mRNA sequence

ATGGATTTGGAAAGATCACTCGTTGTTTCTCGATTGATGGCTCATTATTCTTGGAAGAAAGTTCATATTGCCCTTGAGAATTTCTTTAAATCCTCTGTCTCGATTAACCCCTTCATGGATGATAAAGCTTTGGTTCATGTTGTAGTTGGTGGCCTGGAATTTTCAGCAATTGGTAAGTGGAAGAGATTTGGGGACTTACACTTGAAAATGGAGTTTTGGTCCTTTGATATTCATTCACAGCCGAAGGTTATAAAAAGTTATGGAGGGTGGATAGCTATTAGAAATCTTCCACTGGATTTATGGCATCGTGATTCCTTTGAGGCTATTGGAAAGAATCTTGGAGGGTTGATTGGTAGCAAGTATGAATTTTCATTAAGATATGGTGATATTAATTCTTTAGAAACCAGAAATCTGAATCTTGATTCAAGAAAAATGCTCAATGTCAATGACTTTTCAAATTCCCTGGATTCAAGTAGGGTTAGGAAAGTAATTTTGGATGAAGATTCTGATAACAAAAATGATGGGGAAGGGATGAGTGAAAGCCATCTAAATCTCGAGCCTTCATCCGAAAAGTTAGCATTGAATAGGGAGTCTTCATTGGCATTAAATCTGGAGCATTCTTCTGAAGTTATCCCTATTTTGTCTCCAATTTTAAATAATCACTCTTTCAATCATTTGTTGCCTGATGTTCCCAAGACGACTAAGGAAATTCTTCCTCCTTTATTCGGTGACTCTTCAAGTCCACAAAGCATTCAAGATGTCAAAAATAAATTCATTCAGACCTATTCTCATTTTTATACTCGGAAGAAGAAAGAAGTTGCTTCATCATCTATTGCTAAGGATTCTCACGGTAATCCAGATGCTATTGAAGAGGCCTGTTCTTTATTATTGGCTCCTTCTCTTAAGCAAGCTTCAGATGTCTTCATCAAGGGTATTTTTTCTCATGATCAAAATGTTTCTATTAATTGTCCAATTTCTATCTCTTGTATGGAAGAATCTGATGTGGCTTCTGATGTCAGCTTAAGTAGTGAAGAATTTGAACCACATTCTATGAGTCAACCTAATGATACCATTATTGAAGACACTATTGAAGACACTTATGCTGAAACTTTGAGATTATTATTTCAGGATGATGAGCAGAACTTTGATCCTCCTAATTTAAATGTTTCTTCTCAATCACCTATAGCTATTCCGGATAATTTCTCTTCGTTGGTTGATGCGTGTGGTTTCCAGATTAAAGAAATTCCTCCTTTAAATCCAAAGACAATTCAGTAA

Coding sequence (CDS)

ATGGATTTGGAAAGATCACTCGTTGTTTCTCGATTGATGGCTCATTATTCTTGGAAGAAAGTTCATATTGCCCTTGAGAATTTCTTTAAATCCTCTGTCTCGATTAACCCCTTCATGGATGATAAAGCTTTGGTTCATGTTGTAGTTGGTGGCCTGGAATTTTCAGCAATTGGTAAGTGGAAGAGATTTGGGGACTTACACTTGAAAATGGAGTTTTGGTCCTTTGATATTCATTCACAGCCGAAGGTTATAAAAAGTTATGGAGGGTGGATAGCTATTAGAAATCTTCCACTGGATTTATGGCATCGTGATTCCTTTGAGGCTATTGGAAAGAATCTTGGAGGGTTGATTGGTAGCAAGTATGAATTTTCATTAAGATATGGTGATATTAATTCTTTAGAAACCAGAAATCTGAATCTTGATTCAAGAAAAATGCTCAATGTCAATGACTTTTCAAATTCCCTGGATTCAAGTAGGGTTAGGAAAGTAATTTTGGATGAAGATTCTGATAACAAAAATGATGGGGAAGGGATGAGTGAAAGCCATCTAAATCTCGAGCCTTCATCCGAAAAGTTAGCATTGAATAGGGAGTCTTCATTGGCATTAAATCTGGAGCATTCTTCTGAAGTTATCCCTATTTTGTCTCCAATTTTAAATAATCACTCTTTCAATCATTTGTTGCCTGATGTTCCCAAGACGACTAAGGAAATTCTTCCTCCTTTATTCGGTGACTCTTCAAGTCCACAAAGCATTCAAGATGTCAAAAATAAATTCATTCAGACCTATTCTCATTTTTATACTCGGAAGAAGAAAGAAGTTGCTTCATCATCTATTGCTAAGGATTCTCACGGTAATCCAGATGCTATTGAAGAGGCCTGTTCTTTATTATTGGCTCCTTCTCTTAAGCAAGCTTCAGATGTCTTCATCAAGGGTATTTTTTCTCATGATCAAAATGTTTCTATTAATTGTCCAATTTCTATCTCTTGTATGGAAGAATCTGATGTGGCTTCTGATGTCAGCTTAAGTAGTGAAGAATTTGAACCACATTCTATGAGTCAACCTAATGATACCATTATTGAAGACACTATTGAAGACACTTATGCTGAAACTTTGAGATTATTATTTCAGGATGATGAGCAGAACTTTGATCCTCCTAATTTAAATGTTTCTTCTCAATCACCTATAGCTATTCCGGATAATTTCTCTTCGTTGGTTGATGCGTGTGGTTTCCAGATTAAAGAAATTCCTCCTTTAAATCCAAAGACAATTCAGTAA

Protein sequence

MDLERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALVHVVVGGLEFSAIGKWKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGLIGSKYEFSLRYGDINSLETRNLNLDSRKMLNVNDFSNSLDSSRVRKVILDEDSDNKNDGEGMSESHLNLEPSSEKLALNRESSLALNLEHSSEVIPILSPILNNHSFNHLLPDVPKTTKEILPPLFGDSSSPQSIQDVKNKFIQTYSHFYTRKKKEVASSSIAKDSHGNPDAIEEACSLLLAPSLKQASDVFIKGIFSHDQNVSINCPISISCMEESDVASDVSLSSEEFEPHSMSQPNDTIIEDTIEDTYAETLRLLFQDDEQNFDPPNLNVSSQSPIAIPDNFSSLVDACGFQIKEIPPLNPKTIQ
Homology
BLAST of Lag0042064 vs. NCBI nr
Match: KAA0038211.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 92.0 bits (227), Expect = 1.3e-14
Identity = 41/94 (43.62%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 25  LENFFKSSVSINPFMDDKALVHVVVGGLEFSAI--GKWKRFGDLHLKMEFWSFDIHSQPK 84
           LE+ F+  ++INPF D++A   +  G +E   +   KW  +G  HL  E W    HS+P 
Sbjct: 172 LEDLFEVKITINPFFDNRATNKINHGKIENLVVTQDKWFNYGKFHLLFEKWDVINHSRPS 231

Query: 85  VIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGL 117
            IK + GW++I+NLPLDLW R  FE IG + GGL
Sbjct: 232 SIKGFRGWLSIKNLPLDLWRRAIFEVIGAHFGGL 265

BLAST of Lag0042064 vs. NCBI nr
Match: TYK30603.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 92.0 bits (227), Expect = 1.3e-14
Identity = 41/94 (43.62%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 25  LENFFKSSVSINPFMDDKALVHVVVGGLEFSAI--GKWKRFGDLHLKMEFWSFDIHSQPK 84
           LE+ F+  ++INPF D++A   +  G +E   +   KW  +G  HL  E W    HS+P 
Sbjct: 172 LEDLFEVKITINPFFDNRATNKINHGKIENLVVTQDKWFNYGKFHLLFEKWDVINHSRPS 231

Query: 85  VIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGL 117
            IK + GW++I+NLPLDLW R  FE IG + GGL
Sbjct: 232 SIKGFRGWLSIKNLPLDLWRRAIFEVIGAHFGGL 265

BLAST of Lag0042064 vs. NCBI nr
Match: XP_038904899.1 (uncharacterized protein LOC120091119 isoform X2 [Benincasa hispida])

HSP 1 Score: 89.4 bits (220), Expect = 8.3e-14
Identity = 34/59 (57.63%), Postives = 44/59 (74.58%), Query Frame = 0

Query: 58  GKWKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGL 117
           GKW++FG  HLK E W+  IH +P  ++ YGGWI+I+NLPLD W + +FEAIGK  GGL
Sbjct: 222 GKWQKFGSFHLKFERWNNSIHGRPAYVRGYGGWISIKNLPLDYWCKQTFEAIGKYFGGL 280

BLAST of Lag0042064 vs. NCBI nr
Match: KAA0047189.1 (hypothetical protein E6C27_scaffold83G00690 [Cucumis melo var. makuwa] >TYK29739.1 hypothetical protein E5676_scaffold1280G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 87.0 bits (214), Expect = 4.1e-13
Identity = 56/186 (30.11%), Postives = 91/186 (48.92%), Query Frame = 0

Query: 2   DLERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALVHVVVGGLE--FSAIGK 61
           + E   ++++L A    +K+   LEN+F++ + INP  D+ AL+++  G ++      GK
Sbjct: 62  NFENLWIITKLFAFDDRRKIRKMLENYFQAKIVINPLFDENALINLDEGSIKDLICNEGK 121

Query: 62  WKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFE-------AIGKN 121
           W+  G  +LK E W    +S+P  +K YGGW+ I+NL   LW   + E        +  N
Sbjct: 122 WQVLGSFYLKFEKWDKHKYSRPLTMKGYGGWLKIKNL---LWITGAGEPSSEARIQVKHN 181

Query: 122 LGGLIGSKYE--------FSLRYGDINSLETRNLNLDSRKMLNVNDFSNSLDSSRVRKVI 171
           L G + S  E          L +GD   L   N    +   + V+DF  S+   R+ +V+
Sbjct: 182 LCGFVPSTIEIIDPKRGNIFLNFGDFEFL---NPPFPTSDTIMVSDFKKSIHLLRIMEVL 241

BLAST of Lag0042064 vs. NCBI nr
Match: KAA0035766.1 (hypothetical protein E6C27_scaffold403G00440 [Cucumis melo var. makuwa])

HSP 1 Score: 86.3 bits (212), Expect = 7.1e-13
Identity = 39/119 (32.77%), Postives = 66/119 (55.46%), Query Frame = 0

Query: 1   MDLERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALV--HVVVGGLEFSAIG 60
           + LE ++V++R + H  WK +  +L+   K+S + NPF  +KAL+            +  
Sbjct: 134 LPLENAVVITRRLFHDDWKVIMNSLKKQTKTSFTYNPFHAEKALIIFSEHQNAKSLCSNT 193

Query: 61  KWKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGLI 118
           +W       +K EFWS + H+  K+I SYGGW++ R +P+  W+ D+F  IG +  GL+
Sbjct: 194 RWTTISKFQVKFEFWSIEKHAGQKLIPSYGGWMSFRGIPIHTWNIDTFLQIGNSSEGLL 252

BLAST of Lag0042064 vs. ExPASy TrEMBL
Match: A0A5A7T996 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G00480 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.2e-15
Identity = 41/94 (43.62%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 25  LENFFKSSVSINPFMDDKALVHVVVGGLEFSAI--GKWKRFGDLHLKMEFWSFDIHSQPK 84
           LE+ F+  ++INPF D++A   +  G +E   +   KW  +G  HL  E W    HS+P 
Sbjct: 172 LEDLFEVKITINPFFDNRATNKINHGKIENLVVTQDKWFNYGKFHLLFEKWDVINHSRPS 231

Query: 85  VIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGL 117
            IK + GW++I+NLPLDLW R  FE IG + GGL
Sbjct: 232 SIKGFRGWLSIKNLPLDLWRRAIFEVIGAHFGGL 265

BLAST of Lag0042064 vs. ExPASy TrEMBL
Match: A0A5D3E3A5 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00110 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.2e-15
Identity = 41/94 (43.62%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 25  LENFFKSSVSINPFMDDKALVHVVVGGLEFSAI--GKWKRFGDLHLKMEFWSFDIHSQPK 84
           LE+ F+  ++INPF D++A   +  G +E   +   KW  +G  HL  E W    HS+P 
Sbjct: 172 LEDLFEVKITINPFFDNRATNKINHGKIENLVVTQDKWFNYGKFHLLFEKWDVINHSRPS 231

Query: 85  VIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGL 117
            IK + GW++I+NLPLDLW R  FE IG + GGL
Sbjct: 232 SIKGFRGWLSIKNLPLDLWRRAIFEVIGAHFGGL 265

BLAST of Lag0042064 vs. ExPASy TrEMBL
Match: A0A5A7U128 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1280G00090 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-13
Identity = 56/186 (30.11%), Postives = 91/186 (48.92%), Query Frame = 0

Query: 2   DLERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALVHVVVGGLE--FSAIGK 61
           + E   ++++L A    +K+   LEN+F++ + INP  D+ AL+++  G ++      GK
Sbjct: 62  NFENLWIITKLFAFDDRRKIRKMLENYFQAKIVINPLFDENALINLDEGSIKDLICNEGK 121

Query: 62  WKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFE-------AIGKN 121
           W+  G  +LK E W    +S+P  +K YGGW+ I+NL   LW   + E        +  N
Sbjct: 122 WQVLGSFYLKFEKWDKHKYSRPLTMKGYGGWLKIKNL---LWITGAGEPSSEARIQVKHN 181

Query: 122 LGGLIGSKYE--------FSLRYGDINSLETRNLNLDSRKMLNVNDFSNSLDSSRVRKVI 171
           L G + S  E          L +GD   L   N    +   + V+DF  S+   R+ +V+
Sbjct: 182 LCGFVPSTIEIIDPKRGNIFLNFGDFEFL---NPPFPTSDTIMVSDFKKSIHLLRIMEVL 241

BLAST of Lag0042064 vs. ExPASy TrEMBL
Match: A0A5A7T0M9 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00440 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.4e-13
Identity = 39/119 (32.77%), Postives = 66/119 (55.46%), Query Frame = 0

Query: 1   MDLERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALV--HVVVGGLEFSAIG 60
           + LE ++V++R + H  WK +  +L+   K+S + NPF  +KAL+            +  
Sbjct: 134 LPLENAVVITRRLFHDDWKVIMNSLKKQTKTSFTYNPFHAEKALIIFSEHQNAKSLCSNT 193

Query: 61  KWKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGLI 118
           +W       +K EFWS + H+  K+I SYGGW++ R +P+  W+ D+F  IG +  GL+
Sbjct: 194 RWTTISKFQVKFEFWSIEKHAGQKLIPSYGGWMSFRGIPIHTWNIDTFLQIGNSSEGLL 252

BLAST of Lag0042064 vs. ExPASy TrEMBL
Match: A0A5D3DKV0 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00050 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.5e-13
Identity = 45/118 (38.14%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 3   LERSLVVSRLMAHYSWKKVHIALENFFKSSVSINPFMDDKALVHV---VVGGLEFSAIGK 62
           LE ++V+ R   H  W K+   L    + S + N F  +KALVH    +   L     G 
Sbjct: 200 LENTVVIVRRFFHDDWHKILQNLRKQTEESFTYNAFHAEKALVHFSSNIPANLLCQNKG- 259

Query: 63  WKRFGDLHLKMEFWSFDIHSQPKVIKSYGGWIAIRNLPLDLWHRDSFEAIGKNLGGLI 118
           W   G   +K E WS   H+ PK+I SYGGW   R +PL LW+  +F+ +GK  GGLI
Sbjct: 260 WTTVGKYSVKFEKWSSAYHATPKLIPSYGGWTTFRGIPLHLWNMTTFQQLGKACGGLI 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0038211.11.3e-1443.62Ulp1-like peptidase [Cucumis melo var. makuwa][more]
TYK30603.11.3e-1443.62Ulp1-like peptidase [Cucumis melo var. makuwa][more]
XP_038904899.18.3e-1457.63uncharacterized protein LOC120091119 isoform X2 [Benincasa hispida][more]
KAA0047189.14.1e-1330.11hypothetical protein E6C27_scaffold83G00690 [Cucumis melo var. makuwa] >TYK29739... [more]
KAA0035766.17.1e-1332.77hypothetical protein E6C27_scaffold403G00440 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7T9966.2e-1543.62Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G... [more]
A0A5D3E3A56.2e-1543.62Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold8466... [more]
A0A5A7U1282.0e-1330.11Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7T0M93.4e-1332.77DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3DKV04.5e-1338.14DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025558Domain of unknown function DUF4283PFAMPF14111DUF4283coord: 63..118
e-value: 7.4E-7
score: 28.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0042064.1Lag0042064.1mRNA