Lag0041117 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041117 vs. NCBI nr
Match: KAG7031483.1 (S-protein-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 184.1 bits (466), Expect = 8.8e-43
Identity = 87/150 (58.00%), Postives = 109/150 (72.67%), Query Frame = 0

Query: 1   MHQLVLLLLGLLFFVVSRPTNASDDVSRPNE--SLIQVSKYRVEIHNEL-IYTLDSHCFS 60
           M +LVLL LGLL  +    T AS++  +  +  SL+ + K+ VEI N L +Y LDSHCFS
Sbjct: 183 MDRLVLLWLGLLLMMSCPTTGASEEQQQQQQERSLVDIPKFHVEIRNGLKMYILDSHCFS 242

Query: 61  KDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVREFCPNF 120
           KDDDLGLHIL P+ QQ WSF  N++G TLFHCRLEW+RGF EFDAFFVD+ F  E+C NF
Sbjct: 243 KDDDLGLHILFPDDQQSWSFRDNFWGRTLFHCRLEWERGFKEFDAFFVDEVFFDEYCSNF 302

Query: 121 LCVWLAKHDGLYMVNATGQQVLNQYWELLR 148
            C+W+AK DG+YM+N  GQ+V    W+LLR
Sbjct: 303 RCIWIAKQDGIYMLNKAGQEVFYHRWKLLR 332

BLAST of Lag0041117 vs. NCBI nr
Match: XP_023546939.1 (S-protein homolog 74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 178.7 bits (452), Expect = 3.7e-41
Identity = 80/129 (62.02%), Postives = 97/129 (75.19%), Query Frame = 0

Query: 20  TNASDDVSRPNESLIQVSKYRVEIHNEL-IYTLDSHCFSKDDDLGLHILSPNQQQDWSFS 79
           T AS++  +   SLI + K+RVEI N L +Y LDSHCFSKDDDLGLHIL P+ QQ WSF 
Sbjct: 2   TGASEEQQQQERSLIDIPKFRVEIRNGLQMYILDSHCFSKDDDLGLHILFPDDQQSWSFR 61

Query: 80  INYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVREFCPNFLCVWLAKHDGLYMVNATGQQV 139
            N +G TLFHCRLEW+RGF EFDAFFVD+ F  E+C NF C+W+AK DG+YM+N  GQ+V
Sbjct: 62  DNIWGRTLFHCRLEWERGFKEFDAFFVDEVFFDEYCSNFRCIWIAKQDGIYMLNKAGQEV 121

Query: 140 LNQYWELLR 148
               W+LLR
Sbjct: 122 FYHRWKLLR 130

BLAST of Lag0041117 vs. NCBI nr
Match: KAG6600849.1 (S-protein-like 74, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 176.8 bits (447), Expect = 1.4e-40
Identity = 78/129 (60.47%), Postives = 96/129 (74.42%), Query Frame = 0

Query: 20  TNASDDVSRPNESLIQVSKYRVEIHNEL-IYTLDSHCFSKDDDLGLHILSPNQQQDWSFS 79
           T AS +  +   SL+ + K+ VEI N L +Y LDSHCFSKDDDLGLHIL P+ QQ WSF 
Sbjct: 7   TGASKEQQQQERSLVDIPKFHVEIRNGLKMYILDSHCFSKDDDLGLHILFPDDQQSWSFR 66

Query: 80  INYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVREFCPNFLCVWLAKHDGLYMVNATGQQV 139
            N++G TLFHCRLEW+RGF EFDAFFVD+ F  E+C NF C+W+AK DG+YM+N  GQ+V
Sbjct: 67  DNFWGRTLFHCRLEWERGFKEFDAFFVDEVFFDEYCSNFRCIWIAKQDGIYMLNKAGQEV 126

Query: 140 LNQYWELLR 148
               W+LLR
Sbjct: 127 FYHRWKLLR 135

BLAST of Lag0041117 vs. NCBI nr
Match: KAE8648168.1 (hypothetical protein Csa_018354 [Cucumis sativus])

HSP 1 Score: 160.6 bits (405), Expect = 1.0e-35
Identity = 66/97 (68.04%), Postives = 81/97 (83.51%), Query Frame = 0

Query: 51  LDSHCFSKDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDFV 110
           +D HC+SKD+DLGL IL P+++Q W F  N+ GTTLFHCRLEW+RGF EFDAFFVD+DFV
Sbjct: 1   MDCHCWSKDNDLGLQILLPDERQSWPFRRNFMGTTLFHCRLEWERGFREFDAFFVDEDFV 60

Query: 111 REFCPNFLCVWLAKHDGLYMVNATGQQVLNQYWELLR 148
            +FCPNFLCVW+AK DGLYM+N  GQ V + +W+LLR
Sbjct: 61  NQFCPNFLCVWIAKQDGLYMLNEAGQLVFHHHWKLLR 97

BLAST of Lag0041117 vs. NCBI nr
Match: KAE8648167.1 (hypothetical protein Csa_018435 [Cucumis sativus])

HSP 1 Score: 157.9 bits (398), Expect = 6.8e-35
Identity = 72/149 (48.32%), Postives = 103/149 (69.13%), Query Frame = 0

Query: 4   LVLLLLGLLFFVVSRP----TNASDDVSRPNESLIQVSKYRVEIHNEL-IYTLDSHCFSK 63
           LV+LLL  L   +++      + +  +S PN  ++   ++ VEIHN+L ++ LDSHC+SK
Sbjct: 9   LVILLLAPLRTSMAKKKAKNKSPTPKLSVPNNPVVMFERFTVEIHNDLRMFILDSHCYSK 68

Query: 64  DDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVREFCPNFL 123
           DDDLGLHIL P+++QDWSF  N+  TT FHCRLEW+ G++EFD+F    DFV  +C N  
Sbjct: 69  DDDLGLHILFPDEKQDWSFKGNWIATTSFHCRLEWEVGYLEFDSFKSAPDFVTNYCGNQT 128

Query: 124 CVWLAKHDGLYMVNATGQQVLNQYWELLR 148
           C+W A+ DG+Y+ NA G+QV   YWE++R
Sbjct: 129 CIWSARQDGVYLNNAAGEQVFYNYWEMIR 157

BLAST of Lag0041117 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.1e-11
Identity = 45/132 (34.09%), Postives = 66/132 (50.00%), Query Frame = 0

Query: 6   LLLLGLLFFVVSRPTNA--SDDVSRPNE-----SLIQVSKYRVEIHNEL--IYTLDSHCF 65
           L L  L+ F+ ++ + A   +D+  PN+     S+   SK  VEI+N+L    TL  HC 
Sbjct: 8   LSLFILIIFITTKLSQADHKNDIPVPNDPSSTNSVFPTSKRTVEINNDLGNQLTLLYHCK 67

Query: 66  SKDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVRE-FCP 125
           SKDDDLG   L P +   +SF   +FG TL+ C   W      FD +   +D   +  C 
Sbjct: 68  SKDDDLGNRTLQPGESWSFSFGRQFFGRTLYFCSFSWPNESHSFDIYKDHRDSGGDNKCE 127

Query: 126 NFLCVWLAKHDG 128
           +  CVW  + +G
Sbjct: 128 SDRCVWKIRRNG 139

BLAST of Lag0041117 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.2e-11
Identity = 40/114 (35.09%), Postives = 62/114 (54.39%), Query Frame = 0

Query: 35  QVSKYRVEIHNELI--YTLDSHCFSKDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLE 94
           ++S+++V + N L    TL  HC SK+DDLG   L    +  W+F  N   +T F C + 
Sbjct: 37  KISEWQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMN 96

Query: 95  WQRGFIEFDAFFVDQDFVREFCPNFLCVWLAKHDGLYMVN-ATGQQVLNQYWEL 146
              G +  + F+ D   +   C    C+W AK DGLY+ N A+G+ VL++ WE+
Sbjct: 97  KDNGHMNVNVFW-DDVILFHRCGWKNCIWTAKTDGLYLWNSASGEDVLSRKWEV 149

BLAST of Lag0041117 vs. ExPASy Swiss-Prot
Match: P0DN92 (S-protein homolog 24 OS=Arabidopsis thaliana OX=3702 GN=SPH24 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.2e-11
Identity = 36/93 (38.71%), Postives = 53/93 (56.99%), Query Frame = 0

Query: 40  RVEIHNELIYTLDSHCFSKDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRL---EWQRG 99
           RV I N+  Y L  HC S+DDDLG HIL+  +   W F +N+  +TL+ C     + ++G
Sbjct: 47  RVTIQNDNDYLLGIHCKSRDDDLGFHILAKGELFGWKFHVNFCYSTLYFCGFSQGQMKKG 106

Query: 100 FIEFDAFFVDQDFVREFCPNFLCVWLAKHDGLY 130
              F+ +  ++DF R  C N  C W A+ DG+Y
Sbjct: 107 V--FEIYRANRDFYR--CAN--CTWKAEKDGIY 133

BLAST of Lag0041117 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.5e-10
Identity = 45/133 (33.83%), Postives = 69/133 (51.88%), Query Frame = 0

Query: 15  VVSRPTNASDDVSRPNESLIQVSKYRVEIHNELI--YTLDSHCFSKDDDLGLHILSPNQQ 74
           V++R T   D V      + ++S+++V + N L    TL  HC SK++DLG   L    +
Sbjct: 23  VLARDTTTRDIV------VPKISEWQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDR 82

Query: 75  QDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDFVREFCPNFLCVWLAKHDGLYMVN 134
             W+F  N   +TLF C +    G +    F+ D   +   C    CVW AK+DGLY+ N
Sbjct: 83  FSWNFGENMLHSTLFWCYMSKDDGHMNVKVFW-DDVILFHRCDWKNCVWTAKNDGLYLWN 142

Query: 135 -ATGQQVLNQYWE 145
            A G+ VL++ W+
Sbjct: 143 SAIGEDVLSEKWK 148

BLAST of Lag0041117 vs. ExPASy Swiss-Prot
Match: Q9FMQ4 (S-protein homolog 3 OS=Arabidopsis thaliana OX=3702 GN=SPH3 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 7.8e-10
Identity = 31/72 (43.06%), Postives = 39/72 (54.17%), Query Frame = 0

Query: 50  TLDSHCFSKDDDLGLHILSPNQQQDWSFSINYFGTTLFHCRLEWQRGFIEFDAFFVDQDF 109
           TL+ HC S DDDLGL IL+PN    + F  +  GTTLF+C   W      FD +  D+D 
Sbjct: 50  TLNLHCKSADDDLGLKILAPNGSWSFKFRTSIVGTTLFYCHFTWPGQSKRFDIYDDDRDG 109

Query: 110 VREFCPNFLCVW 122
           VR       C+W
Sbjct: 110 VRSHISCINCIW 121