Lag0041066 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0041066
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCytochrome P450
Locationchr13: 11504353 .. 11508845 (-)
RNA-Seq ExpressionLag0041066
SyntenyLag0041066
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGGGCTCTACTTGGTGATTTGGTGTCGATTTTTCCGGCAGGGGTTGCGATGGCGGTGGTGGCAGTGTTGGTCCATTTCTACTATGGGGTGTGGAGAAGGACGGCGGAGGTGAGGAGGAAGCTTGGAGCGCAGGGGGTGACGGGTCCGCCACCGTCGTCCGCTCTTTTTGGAAACTTGCCAGAGATGAAGGAGATCGAGTTGCAGGCGGCGGCCATGGCTTCTCCGCCGCATCATCCGTCGCCAACTGTAGCTCATGATTATATCTCCACCCTCTTTCCCTATTTCGTCAAGTGGAGGAAGCAATACGGTATTTTTCTACCTTTTGTTTTTCGAGTTAAATTATAAATTTGATACCAAGTGTATTGATCTTGTGTTTTTATTTGTTTGATTTTTTTTTTTTAAAAAAAGTTTTTAATTAGATCTATGAATATTCAACTTTCAAATGTTTTAATATTCTTTAAAAGAAAATTTGTCTTTAAACTTTGGAGGAAAAAAGCATTGGTCCGTTTGATAACTATTTGGTTTTTAAAAATTAAGCTTGTTTTCTCCTCATTATGATGTTTTACATCTTTGTTAAGGACACATTTGAATTCCTTGCCAGATTTAAAAAATAAAAACAAGTTTTTGGAAACTATTTTTTTTTTTTTCAAAATTTGATTTGGTTTTTGAAAACAAGGAAAAAACTAATACTAAAACAAAGAAACTCATGTGTAGGTATAAACTTAATTTTCAAAAACTAAAAACCAACGACCAAATGGTTATCAAACGGGACCTCAAATGATGATGGGTCAAAATTTTCAAAAACTAAAAACAAAAAACCAAACCGTTATCAAATGGATCTAAATAGCTATGTGATACTTATCCAAAATACTTTTGTTTTCTCTTTCACACTTGGAAACTTTTAACCGTTGAGCTATGCGAATGTGAGCACCGAGAGAGGAAAATGGAATGAAATTCTCTTAAAAAATTGTGGCAGGCCCATTGTACACGTATTCAACAGGGACGAGGCAGCATCTGTACGCCAACGATGTGGATCTGGTGAAGGACTTGAGCCTCTCCAAGAATCCCGACCTGGACAAGCCACCCTACATCTTCAAGAAGTTCGCGCCGCTCCTCGGCCACGGCGTCATCCTCTCCAACGGCGCCGACTGGACGCTGCAGCACAAGATCATCGCCCCGGAGTTCTTCATGGACCGGGTCCGGGGCATGGCGGCGCTGATGGTGGAGGCGGCGGCGCCGATGCTGGACAAATGGGAGGCCAGGATCGGAGAAGAAGGAGAGGGAGCGGCGGCGGAGATCGAGGTGGAGGAGGGTCTGCGAGGATTCTCGGCGGATGTGATTTCAAGGGCTTGCTTTGGGAGCTCCTATGAGAAAGGGAAGCACATTTTTTCAAAACTTAGGGATCTTCAGAAGTTGATCAGCCACGGAAACCCTCTGTTTCGCTCTTCTTTCAGGTTTCTTTCTCTCTATAAACAATTTTCATACAAAACTATGTTTTCATTTATTTTTTTTTTTTCAATAGGATAGTCGTTGATTCCTTATTTTATTAATATATTTGGAAATCGTGTGCCCGTACAAGTTTTCTTGACCGTAAACTTGTTAAGCTATATCAATTTTTGGACTATAAACTTAAATATATAAGTGCGGCAATTTTCACTCTCTATTCAATTTCACATAATTCACGTACTAATGTTATACAATGTAAAAATTTTCATCAATTCATTAATTTCAATAAAATAAAACTTTACACAAACATGATTCTATAACCAATGTTGAAATTAACTAGGAAATAACTTTGCAAAAATTCATAAATTCAAGGAAATATCCTAGCACGGGTGTCATGATCAATTTCATATCCTACGTATGATTTATAATTTCTTGACAACACATATATGATCAAATTTTTTATAAAGATATTTAGGGCGAGAACTTATTAAAATGATCAAAATTGTAACACTTTTTAAAGTTTAAGGTTAATATTGCAATACTTATGTAAGTTTAAGGTGCAATTTGATGAGAAATTGAAAGTTTAGGATCTATATATTGTTACAATCTAAACCATTTTAGATCTAATTAAATTGATTTGTTATCTTCTTTTTTAAGTTGAACAACGACTCATGAGAAAGTGAGAATTTGATAGTTCTTAGCTCTTACCCAATTTAGCTACAGTCGATAAGTTATGGACTTGACACTTAAACATCCTTTGGAAGAAATTTAATTTATTTACGGGAGAATTAAGATCTTCCGGACGAGTCGAATTTTGTTCCAATTAATATGTGAGTGGTGGTATGTTTATATGAACATGGTTAAAGCATAAGTTAACTTTTGAAATGTTTAGATGGTTTTTGCTTTTATGGAAAAAAAAAGTTATTTATTGGTTTATGGGACCTATCTATTTATTATTATTAATTTTAAATTAAAATTTGGTCCACTTATTTATTTTATACCATAGAATTAGACTAATAAAAATAAAGGGAGAAATACACTTTTGGTCCCTAAAGTTTGGGTTAAGTGACACTTTGGTCCCTGAGTTTTCAAACTCAACAAAAATACCCATGAGGTTTGAGATAAGTAGCACTTTGGTCCCTGCCGTCCATTTTGTCGTTAAACCCTAATAGTTTGTTGACGTGACACTATTATTTTAATTAAAAAAATGAATTTTTAATTTTTTTAAATAAAAATATATAAAAATATTAAATATATAAAAGAATGGAGTTTCGGGGAGGAGGAATATATTTAATATTTTTATATATTTTTATTTAAAAAAATTAAAAATTCATTTTTTTAATTAAAATAATAGTGTCACGTCAGCAAACTATTAGGGTCTAACGGCAAAATGGACGGCAGGGACCAAAGTGTTACTTATCTCAAACCTTAGGGGTATTTTTGTTGAGTTTGAAAATTCAGAGACCAAAGTGTCACTTAACCCAAACTTCAGGGACCAAAAGTGTATTTTTTCCAAAAATAAATTACATTAGAAAGTTGGTAGAAGCTCCTAAACAAGTTTGTAGGACTAATATCATTGTGTAAGATTTAACGAGTTAAGTTATGTTTAGATAGCCAGAAGGATCAATTACCGTTAAAGGTTGGACTAAAAAGGAAAAAAATAAATAAAATGGTGAACAGTGACAGATTTTTTCAACCAAGCAAACACAGAAGAATAAAAAAGCTGGAGAAGGAGATCGAGTCATTGATATGGGAGACGGTTCAGCAACGGCAGGAAGAAAGCTCCAAAACGTCGTCGTTGGAGAAGGATCTGTTACAGGTTTTAATGGAAGGCGCCATGAACGACCCAACTATAGGAGCTTGCAATGAGTCCTGCAAGAAGTTCATCGTTGACAACTGCAAGACCATGTACTTTGCCGGCCATGAGTCCACCGCGGCCACCGCCACTTGGGCCTTAATGCTCCTTGCCCTCCACCCTGAATGGCAAGATCGTATCCGCTCTGAGTTCGCTCTAGCTTGCCCCGATGGTCGCCTCGACACGACCGCAATCTCCCAACTCAAATCGGTATCCCTCCTCCTACCCGAACTAAATCATTAATTGAAGTTTTATGCACATTCGTTCCAAATGAGATGGGGTCTGTGATACTTTTCAAAATAAGGTCTTAAAATATTTTATAGCAAAGACAAAATTTTAAAATATCAAAATTGATTTGTATTTTACGATTTTTTTTTTACAAAAAAGAAAACAAAAAAAATATATGAAACTAAGAAAATATCACCAAACAACTATACGATAGTGATATTTTATTTAAAAAGTGTCATTTTTCGAACTAGATTAAAAAGTTTTATGAGAAATGACAAAATTAAGAATGATTCGGAAGAGTGCTTCTTGACGAGGAGAGAGAAATGAAGAGGTGAGAAATTGAAATTTCTTGTCTACCTTTTTCAAAAAAATTTGTTTGTTAAGAACATTTTCAATTTGGGGTATCTGAATTTTGGAGTTACGTGATGAGTCACAATACACACATACTAAAGGACGTCCTTGTTTTTATGATTATAGTATAGATGCAAAAAGTATTTTCTTAACCTTAACCTGAGATAATCATTGGGTGTAGCTGAGTATGGTGGTTCAAGAAACGTTGCGACTATACCCACCAGGGGCATACGTGGCAAGGGAGACATTTGCTGAGACACGACTTGGGAACGTGGTGGTTCCGAAGGGCGTCTGTCTATGGACTTTAATTCCAATGTTGCACACAGAAGTAGAAATATGGGGAGAAGATGCGAATGAGTTCAAACCAGAGCGATTCGCCAAAGGCATAGCCAAGGCCTGCAAGTTTCCTCAGGCCTATTTGCCATTTGGGGCAGGTCATCGGACCTGTTTAGGGAAGAACTTTGCATTGGTTCAATTGAAAATTATCATTTCTTTGGTAGTTTCCAAGTTTAGATTCTCACTATCTCCTGATTATCGTCATTCTCCTTCTCATAGAATAATTGTGGAGCCTGACGATGGTGTGAAGATTGTCTTCCAAAAGCTTTGA

mRNA sequence

ATGGAGGGGGCTCTACTTGGTGATTTGGTGTCGATTTTTCCGGCAGGGGTTGCGATGGCGGTGGTGGCAGTGTTGGTCCATTTCTACTATGGGGTGTGGAGAAGGACGGCGGAGGTGAGGAGGAAGCTTGGAGCGCAGGGGGTGACGGGTCCGCCACCGTCGTCCGCTCTTTTTGGAAACTTGCCAGAGATGAAGGAGATCGAGTTGCAGGCGGCGGCCATGGCTTCTCCGCCGCATCATCCGTCGCCAACTGTAGCTCATGATTATATCTCCACCCTCTTTCCCTATTTCGTCAAGTGGAGGAAGCAATACGGCCCATTGTACACGTATTCAACAGGGACGAGGCAGCATCTGTACGCCAACGATGTGGATCTGGTGAAGGACTTGAGCCTCTCCAAGAATCCCGACCTGGACAAGCCACCCTACATCTTCAAGAAGTTCGCGCCGCTCCTCGGCCACGGCGTCATCCTCTCCAACGGCGCCGACTGGACGCTGCAGCACAAGATCATCGCCCCGGAGTTCTTCATGGACCGGGTCCGGGGCATGGCGGCGCTGATGGTGGAGGCGGCGGCGCCGATGCTGGACAAATGGGAGGCCAGGATCGGAGAAGAAGGAGAGGGAGCGGCGGCGGAGATCGAGGTGGAGGAGGGTCTGCGAGGATTCTCGGCGGATGTGATTTCAAGGGCTTGCTTTGGGAGCTCCTATGAGAAAGGGAAGCACATTTTTTCAAAACTTAGGGATCTTCAGAAGTTGATCAGCCACGGAAACCCTCTGTTTCGCTCTTCTTTCAGAAGAATAAAAAAGCTGGAGAAGGAGATCGAGTCATTGATATGGGAGACGGTTCAGCAACGGCAGGAAGAAAGCTCCAAAACGTCGTCGTTGGAGAAGGATCTGTTACAGGTTTTAATGGAAGGCGCCATGAACGACCCAACTATAGGAGCTTGCAATGAGTCCTGCAAGAAGTTCATCGTTGACAACTGCAAGACCATGTACTTTGCCGGCCATGAGTCCACCGCGGCCACCGCCACTTGGGCCTTAATGCTCCTTGCCCTCCACCCTGAATGGCAAGATCGTATCCGCTCTGAGTTCGCTCTAGCTTGCCCCGATGGTCGCCTCGACACGACCGCAATCTCCCAACTCAAATCGCTGAGTATGGTGGTTCAAGAAACGTTGCGACTATACCCACCAGGGGCATACGTGGCAAGGGAGACATTTGCTGAGACACGACTTGGGAACGTGGTGGTTCCGAAGGGCGTCTGTCTATGGACTTTAATTCCAATGTTGCACACAGAAGTAGAAATATGGGGAGAAGATGCGAATGAGTTCAAACCAGAGCGATTCGCCAAAGGCATAGCCAAGGCCTGCAAGTTTCCTCAGGCCTATTTGCCATTTGGGGCAGGTCATCGGACCTGTTTAGGGAAGAACTTTGCATTGGTTCAATTGAAAATTATCATTTCTTTGGTAGTTTCCAAGTTTAGATTCTCACTATCTCCTGATTATCGTCATTCTCCTTCTCATAGAATAATTGTGGAGCCTGACGATGGTGTGAAGATTGTCTTCCAAAAGCTTTGA

Coding sequence (CDS)

ATGGAGGGGGCTCTACTTGGTGATTTGGTGTCGATTTTTCCGGCAGGGGTTGCGATGGCGGTGGTGGCAGTGTTGGTCCATTTCTACTATGGGGTGTGGAGAAGGACGGCGGAGGTGAGGAGGAAGCTTGGAGCGCAGGGGGTGACGGGTCCGCCACCGTCGTCCGCTCTTTTTGGAAACTTGCCAGAGATGAAGGAGATCGAGTTGCAGGCGGCGGCCATGGCTTCTCCGCCGCATCATCCGTCGCCAACTGTAGCTCATGATTATATCTCCACCCTCTTTCCCTATTTCGTCAAGTGGAGGAAGCAATACGGCCCATTGTACACGTATTCAACAGGGACGAGGCAGCATCTGTACGCCAACGATGTGGATCTGGTGAAGGACTTGAGCCTCTCCAAGAATCCCGACCTGGACAAGCCACCCTACATCTTCAAGAAGTTCGCGCCGCTCCTCGGCCACGGCGTCATCCTCTCCAACGGCGCCGACTGGACGCTGCAGCACAAGATCATCGCCCCGGAGTTCTTCATGGACCGGGTCCGGGGCATGGCGGCGCTGATGGTGGAGGCGGCGGCGCCGATGCTGGACAAATGGGAGGCCAGGATCGGAGAAGAAGGAGAGGGAGCGGCGGCGGAGATCGAGGTGGAGGAGGGTCTGCGAGGATTCTCGGCGGATGTGATTTCAAGGGCTTGCTTTGGGAGCTCCTATGAGAAAGGGAAGCACATTTTTTCAAAACTTAGGGATCTTCAGAAGTTGATCAGCCACGGAAACCCTCTGTTTCGCTCTTCTTTCAGAAGAATAAAAAAGCTGGAGAAGGAGATCGAGTCATTGATATGGGAGACGGTTCAGCAACGGCAGGAAGAAAGCTCCAAAACGTCGTCGTTGGAGAAGGATCTGTTACAGGTTTTAATGGAAGGCGCCATGAACGACCCAACTATAGGAGCTTGCAATGAGTCCTGCAAGAAGTTCATCGTTGACAACTGCAAGACCATGTACTTTGCCGGCCATGAGTCCACCGCGGCCACCGCCACTTGGGCCTTAATGCTCCTTGCCCTCCACCCTGAATGGCAAGATCGTATCCGCTCTGAGTTCGCTCTAGCTTGCCCCGATGGTCGCCTCGACACGACCGCAATCTCCCAACTCAAATCGCTGAGTATGGTGGTTCAAGAAACGTTGCGACTATACCCACCAGGGGCATACGTGGCAAGGGAGACATTTGCTGAGACACGACTTGGGAACGTGGTGGTTCCGAAGGGCGTCTGTCTATGGACTTTAATTCCAATGTTGCACACAGAAGTAGAAATATGGGGAGAAGATGCGAATGAGTTCAAACCAGAGCGATTCGCCAAAGGCATAGCCAAGGCCTGCAAGTTTCCTCAGGCCTATTTGCCATTTGGGGCAGGTCATCGGACCTGTTTAGGGAAGAACTTTGCATTGGTTCAATTGAAAATTATCATTTCTTTGGTAGTTTCCAAGTTTAGATTCTCACTATCTCCTGATTATCGTCATTCTCCTTCTCATAGAATAATTGTGGAGCCTGACGATGGTGTGAAGATTGTCTTCCAAAAGCTTTGA

Protein sequence

MEGALLGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISHGNPLFRSSFRRIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL
Homology
BLAST of Lag0041066 vs. NCBI nr
Match: XP_023516881.1 (cytochrome P450 714A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.0 bits (2070), Expect = 3.1e-228
Identity = 408/532 (76.69%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNLPEM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KP Y+ KK AP+LGH V+ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSVVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFS 245
           ALM EAA+ ML KWE RIGE  +GA AEI+V+E LR FSADVISRACFGSSYEKGK IFS
Sbjct: 181 ALMTEAASAMLQKWEGRIGER-DGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTS 305
           KLR LQKL+S GN LF           +   RRIKKLEKEIESLIWETVQQRQ E SKTS
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQPKHRRIKKLEKEIESLIWETVQQRQRECSKTS 300

Query: 306 SLEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLAL 365
           S +KDLLQ++ME AMNDPT+GA CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLAL
Sbjct: 301 S-DKDLLQLIMEAAMNDPTVGANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEF  ACPDGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+ANEFKPERF  GIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           LGKNFALVQLKIIISL+V KFRFSLSP+YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 LGKNFALVQLKIIISLIVFKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Lag0041066 vs. NCBI nr
Match: KAG6599934.1 (Cytochrome P450 714A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 801.2 bits (2068), Expect = 5.3e-228
Identity = 408/533 (76.55%), Postives = 454/533 (85.18%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNLPEM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLPEMQ 60

Query: 66  EIELQ---AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYAND 125
           EI+L+   AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN 
Sbjct: 61  EIQLEAAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANH 120

Query: 126 VDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGM 185
           VDLVKDLSL+ +  L KP Y+ KK AP+LGH ++ +NG  W LQ KIIAPEFFMDRVR M
Sbjct: 121 VDLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVM 180

Query: 186 AALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIF 245
           AALM EAAA ML KWE RIG EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK IF
Sbjct: 181 AALMTEAAAAMLQKWEERIG-EGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIF 240

Query: 246 SKLRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKT 305
           SKLR LQKL+S GN LF           +S  R IKKLEKEIESLIWETVQQRQ+E SKT
Sbjct: 241 SKLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKT 300

Query: 306 SSLEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLA 365
           SS +KDLLQ++ME AMNDPT+GA CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLA
Sbjct: 301 SS-DKDLLQLIMEAAMNDPTVGANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLA 360

Query: 366 LHPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRL 425
           LHPEWQDRIRSEF  ACPDGR++ T ISQLKS SMVVQETLRLYPP A+VARETF+ETRL
Sbjct: 361 LHPEWQDRIRSEFTQACPDGRVNMTTISQLKSASMVVQETLRLYPPAAFVARETFSETRL 420

Query: 426 GNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRT 485
           GNVV+PKGV LWTLIP LH EVEIWGE+ANEFKPERF  GIAKACKFPQ Y+PFGAG R 
Sbjct: 421 GNVVIPKGVYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQTYVPFGAGPRL 480

Query: 486 CLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           CLGKNFALVQLKIIISL+VSKFRFSLS +YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 CLGKNFALVQLKIIISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 530

BLAST of Lag0041066 vs. NCBI nr
Match: XP_022995469.1 (cytochrome P450 714A1-like [Cucurbita maxima])

HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 405/531 (76.27%), Postives = 452/531 (85.12%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNL EM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLQEMQ 60

Query: 66  EIELQ-AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           EI+L+ AAAM SPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN VD
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSL+    L KP Y+ KK AP+LGH ++ +NG  W LQ KIIAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 LMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSK 245
           LM EAAA ML KWE RIG EGEGA AEI+V+E LR FSADVISRACFGSSYEKGK IFSK
Sbjct: 181 LMTEAAAAMLQKWEGRIG-EGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTSS 305
           LR LQKL+S GN LF           +S  RRIKKLEKEIESLIWETVQQRQ+E SKTSS
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKTSS 300

Query: 306 LEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALH 365
            +KDLLQ++ME AMNDPT+ A CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLALH
Sbjct: 301 -DKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGN 425
           PEWQDRIRSEF  AC DGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLGN
Sbjct: 361 PEWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCL 485
           VV+PKG+ LWTLIP LH EVEIWGE+ANEFKPERF  GIAKACKFPQAYLPFGAG R CL
Sbjct: 421 VVIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCL 480

Query: 486 GKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           GKNFALVQLKI+ISL+VSKFRFSLSP+YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of Lag0041066 vs. NCBI nr
Match: XP_022943066.1 (cytochrome P450 714A1-like isoform X1 [Cucurbita moschata] >XP_022943067.1 cytochrome P450 714A1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 795.4 bits (2053), Expect = 2.9e-226
Identity = 404/532 (75.94%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+    VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGP P S L+GNLPEM+
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPSP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KP Y+ KK AP+LGH ++ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFS 245
           ALM EAAA ML KWE RI +EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK IFS
Sbjct: 181 ALMTEAAAAMLQKWEERI-DEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTS 305
           KLR LQKL+S GN LF           +S  R IKKLEKEIESLIWETVQQRQ+E SKTS
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKTS 300

Query: 306 SLEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLAL 365
           S +KDLLQ++ME AMNDPT+G+ CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLAL
Sbjct: 301 S-DKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEF  ACPDGR++ TAISQLKS SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+AN+FKPERF  GIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           LGKNFALVQLKI+ISL+VSKFRFSLS +YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 LGKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Lag0041066 vs. NCBI nr
Match: XP_038893046.1 (cytochrome P450 714A1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 795.0 bits (2052), Expect = 3.8e-226
Identity = 400/533 (75.05%), Postives = 448/533 (84.05%), Query Frame = 0

Query: 1   MEGALLGDLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG+++   +S+FP  V + VVA+LVH Y YG WRRTAEVRRKL AQGVTGPPP S L+G
Sbjct: 6   MEGSIVCGFISVFPVAVTVTVVAILVHLYFYGAWRRTAEVRRKLRAQGVTGPPP-SILYG 65

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASPPH  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 66  NLPEMQKIQLQTAAMASPPHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 125

Query: 121 ANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N +L KP Y+ KK AP+LGH ++ SNGA W +Q KIIAPEFFMDRV
Sbjct: 126 ANKVELVKDLSLSNNLNLGKPFYVTKKLAPILGHSIVRSNGAIWAVQRKIIAPEFFMDRV 185

Query: 181 RGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R MA LM +AA+ +++KWE+RI   G+GA+ EIEV+E LRGFSADVISRACFGSSYEKGK
Sbjct: 186 RAMAVLMADAASSLVEKWESRI---GDGASVEIEVDEDLRGFSADVISRACFGSSYEKGK 245

Query: 241 HIFSKLRDLQKLISHGNPLFRSS---------FRRIKKLEKEIESLIWETVQQRQEESSK 300
            IFSKLRDLQKLIS G+ LF  S          +RIKKLEKEIESLIWETVQQRQ+E SK
Sbjct: 246 EIFSKLRDLQKLISEGSFLFGYSSLRFLQPRKHKRIKKLEKEIESLIWETVQQRQKEFSK 305

Query: 301 TSSLEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLA 360
           TSS EKDLLQ++ME   NDP +GA N+S K FIVDNCK++YFAGHESTA  ATW LMLLA
Sbjct: 306 TSSSEKDLLQLIMEATTNDPNVGA-NDSSKNFIVDNCKSIYFAGHESTAVAATWTLMLLA 365

Query: 361 LHPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRL 420
           LHPEWQD IRSEFA ACPDGR DTT IS LK++SMVV E LRLYPP A+VARETFA+TRL
Sbjct: 366 LHPEWQDCIRSEFAHACPDGRPDTTVISNLKTVSMVVHEALRLYPPAAFVARETFADTRL 425

Query: 421 GNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRT 480
           GNVVVPKGVCLWTLIP LH EVEIWGEDANEFKPERF  GIAKACKFPQAY+PFGAG R 
Sbjct: 426 GNVVVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFKNGIAKACKFPQAYVPFGAGPRL 485

Query: 481 CLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           CLGKNFALVQLKIIISL+VSKFRFSLSP+Y H PS+R+IVEP +GVKI FQ+L
Sbjct: 486 CLGKNFALVQLKIIISLIVSKFRFSLSPEYCHCPSYRMIVEPANGVKIAFQRL 533

BLAST of Lag0041066 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 3.8e-152
Identity = 275/519 (52.99%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALF-GNLPEMKEIELQAAAMA 76
           V + V+ + +  Y  V      +RRKL  QGV GPPPS  LF GN+PEM++I  Q+  M+
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWRMRRKLTMQGVKGPPPS--LFRGNVPEMQKI--QSQIMS 75

Query: 77  SPPHHPSPT-VAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKN 136
           +  H+     +AHDY S+LFPY   WRKQYG +YTYSTG +QHLY N  +LVK+L+ +  
Sbjct: 76  NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANT 135

Query: 137 PDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPML 196
            +L K  Y+ K+   +LG GVI SNG  W  Q +IIAPEFF+D+V+GM  L+VE+A PML
Sbjct: 136 LNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPML 195

Query: 197 DKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISH 256
            KWE  +  EGE    +I V+E LR  SADVISRACFGSS+ KGK IFSKLR LQK I+H
Sbjct: 196 SKWEEMMKREGE-MVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 GNPLF-------------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQV 316
            N LF             +    +I +LE+ IESLIWETV++R+ E       +KDL+Q+
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 LMEGAMN--DPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRI 376
           ++EGA +  D  +    +S K F+VDNCK++YFAGHE++A   +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGV 436
           R E  L C +G  D  +IS LK+++MV+QETLRLYPP A+V+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALV 496
           C+WTLIP LH + EIWG DANEF PERF++G++KACK PQ+++PFG G R CLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 QLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKI 519
           +LK+++SL+VS+F F+LSP Y+HSP  R++VEP  GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of Lag0041066 vs. ExPASy Swiss-Prot
Match: Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.9e-141
Identity = 255/516 (49.42%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMAS 76
           V + + +V  H Y         +RR L  QGV GPPP S   GN+ EM+ I+ +A   + 
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPP-SIFNGNVSEMQRIQSEAKHCSG 75

Query: 77  PPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPD 136
                   ++HDY S+LFP+F  WRKQYG +YTYSTG +QHLY N  ++VK+LS +   +
Sbjct: 76  -----DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLN 135

Query: 137 LDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDK 196
           L +  +I K+  P+LG+G+I SNG  W  Q +IIA EF  D+++GM  LMVE+A PML+K
Sbjct: 136 LGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNK 195

Query: 197 WEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISHGN 256
           WE  +   GE    +I V+E L+  SADVI++ACFGSS+ KGK IFS +RDL   I+  +
Sbjct: 196 WEEMVKRGGE-MGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS 255

Query: 257 PLFR-SSFR------------RIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQVLM 316
            LFR + F              I  LE E+ES IWETV++R+ E   T   +KDL+Q+++
Sbjct: 256 VLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTH--KKDLMQLIL 315

Query: 317 EGAMNDPTIGACNESC-KKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSE 376
           EGAM        ++S  ++F+VDNCK++YFAGH+STA + +W LMLLAL+P WQ +IR E
Sbjct: 316 EGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDE 375

Query: 377 FALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLW 436
              +C +G  D  +I  LK+++MV+QET+RLYPP   V RE   + RLG++VVPKGVC+W
Sbjct: 376 ILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIW 435

Query: 437 TLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLK 496
           TLIP LH + EIWG DAN+FKPERF++GI+KACK+PQ+Y+PFG G RTC+GKNF ++++K
Sbjct: 436 TLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVK 495

Query: 497 IIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKI 519
           +++SL+VSKF F+LSP Y+HSPSH+++VEP  GV I
Sbjct: 496 VLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Lag0041066 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.6e-126
Identity = 235/511 (45.99%), Postives = 328/511 (64.19%), Query Frame = 0

Query: 22  VAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMASPPHHP 81
           + +  + Y  +W R   +R+KL +QGV GP P S LFGN+PEM+ I+  A +        
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVRGPKP-SFLFGNIPEMRRIQQLAKSAHEQEAGS 79

Query: 82  SPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPP 141
           +   + +Y++TLFPYF+ W + YG +Y YSTG+ Q L   D ++VK+L+  K+ DL KP 
Sbjct: 80  TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDLGKPC 139

Query: 142 YIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDKWEARI 201
           Y+ K+   LLG G++ SNG  W  Q K+IAPE FM+RV+GM  LM+EAA  ML+ W+  +
Sbjct: 140 YLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNEV 199

Query: 202 GEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISHGNPLF-- 261
             E  G +AEI V+E LR FSADVISRACFGSS+ +GK IF K+R LQK ++  + L   
Sbjct: 200 --EDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 259

Query: 262 -------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQVLMEGAMNDPTI 321
                    S R I  L+  I +LI    ++ + +SS  +S+ KDLL  +++G+ + P  
Sbjct: 260 PGSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSS--TSVNKDLLHSIIQGSKDGPFA 319

Query: 322 GACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSEFALACPDGRL 381
               E    FIVDNCK +YFAGHE+T+ TA W LMLLA H EWQ R R E    C    L
Sbjct: 320 SCTPED---FIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPL 379

Query: 382 DTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEV 441
           D   + +LK L+MV+QETLRLYPP ++VARE   + +LG + +PKG  +W  I M H + 
Sbjct: 380 DFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDP 439

Query: 442 EIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKF 501
            +WG  A++F P+RFA GIA ACK P  Y+PFG G RTC G+N A+V+LK+++SL++SKF
Sbjct: 440 SVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKF 499

Query: 502 RFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
            F LSP+Y H P+ R+ +EP  GV ++F++L
Sbjct: 500 EFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of Lag0041066 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.8e-120
Identity = 245/563 (43.52%), Postives = 334/563 (59.33%), Query Frame = 0

Query: 1   MEGALLGDLVSIFPAGVAMAVVAVLVHFYYGVW----RRTAEVRRKLGAQGVTGPPPSSA 60
           ME   +    +  P  VA AV+A L       W    RR AEV R+   QG+ GPPPSS 
Sbjct: 1   MESFFVFFTAAALPVVVAAAVIAGLC--ITAAWLARPRRVAEVFRR---QGIDGPPPSSF 60

Query: 61  LFGNLPEMKEIELQAAAMASPPHHPSPTVA---------------HDYISTLFPYFVKWR 120
           L GNLPEMK     AA+ A+P      T +                DY + +FPYF KWR
Sbjct: 61  LAGNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWR 120

Query: 121 KQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGA 180
           K YG  Y Y    R  LY  D +L+ ++    + D+ KP Y+ K   PL G GV+ +NGA
Sbjct: 121 KAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKGQEPLFGGGVLKANGA 180

Query: 181 DWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGF 240
            W  Q K+IAPEF+M RVR M  LMV+AA P++  WE+RI   G  AAAE+ V+  LR F
Sbjct: 181 CWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSF 240

Query: 241 SADVISRACFGSSYEKGKHIFSKLRDLQKLISHGNPLF---------RSSFRRIKKLEKE 300
           S DVISRACFGS Y +G+ IF +LR+L  L+S  + +F             RRI +L  E
Sbjct: 241 SFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSLRHLPTGKNRRIWRLTGE 300

Query: 301 IESLIWETVQQRQ----------EESSKTSSLEKDLLQVLMEGAMNDPTIGACNESCKKF 360
           I SLI E V++R+           +++  S  E+D L  ++E +   P           F
Sbjct: 301 IRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIENSGGQP-------RPDDF 360

Query: 361 IVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSEFALAC-PDGRL-----DTTA 420
           +VDNCK +YFAGHE++A TATW LMLLA HPEWQDR R+E    C  DG       D   
Sbjct: 361 VVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDM 420

Query: 421 ISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVEIWG 480
           +S+++++ MVVQETLRL+PP ++V RETF + +LG ++ PKG  L+  +  +H +V  WG
Sbjct: 421 VSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWG 480

Query: 481 EDANEFKPERFAKGIAKACKFPQA-YLPFGAGHRTCLGKNFALVQLKIIISLVVSKFRFS 519
             A  F P RF  G+A ACK PQA ++PFG G RTCLG+N ALV++K ++++V+++F F+
Sbjct: 481 PTARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEFT 540

BLAST of Lag0041066 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.1e-116
Identity = 226/510 (44.31%), Postives = 320/510 (62.75%), Query Frame = 0

Query: 26  VHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQA-AAMASPPHHPS-- 85
           V+ Y+ +W    +V  K   Q + GP PS   +GNL +M+E    A AA AS     S  
Sbjct: 25  VYLYHILWLVPQKVLAKFEDQKIGGPRPSFP-YGNLADMREAAAAAKAARASARRSGSGG 84

Query: 86  PTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPPY 145
             + HDY   + PY+ KWRK+YGP++TYS G    L+ +  D+V+D++L  + DL K  Y
Sbjct: 85  GGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCVSLDLGKSSY 144

Query: 146 IFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDKWEARIG 205
           +     PL G G++ SNG  W  Q KIIAPEFF+D+V+GM  LMV++A P+L  WE R+ 
Sbjct: 145 LKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERV- 204

Query: 206 EEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISHGNPL---- 265
            +  G   +I++++ +R +SADVISR CFGSSY KGK IF K+R+LQ+ +S  N L    
Sbjct: 205 -DRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAVSKPNVLAEMT 264

Query: 266 ---FRSSFRRIK--KLEKEIESLIWETVQQRQEESSKTSSLEKDLLQVLMEGAMNDPTIG 325
              F  S R  +  +L K++  LI E V        K S  +++LL  ++  A +   +G
Sbjct: 265 GLRFFPSMRNKQAWELHKQVRKLILEIV--------KESGEDRNLLSAILHSA-STSRVG 324

Query: 326 ACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSEFALACPDGRLD 385
                 + FIVDNCK++YFAGHESTA TA W LMLL LHPEWQ+R+R E    C    +D
Sbjct: 325 IA--EAENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVD 384

Query: 386 TTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVE 445
           + ++ ++K+L+MV+QETLRLYP GA+V+R+   E +LG V +PKGV ++  +  +H + E
Sbjct: 385 SRSLQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPE 444

Query: 446 IWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKFR 505
           +WG D  EF PERF+    +      +YLPFGAG RTCLG+ FA+ +LKI+ISL+VSKF 
Sbjct: 445 LWGPDVKEFNPERFSDVRPQL----HSYLPFGAGARTCLGQGFAMAELKILISLIVSKFV 504

Query: 506 FSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
             LSP Y+HSP+ ++IVEP+ GV +   K+
Sbjct: 505 LKLSPHYQHSPTLKLIVEPELGVDLTLTKV 516

BLAST of Lag0041066 vs. ExPASy TrEMBL
Match: A0A6J1K5V5 (cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 8.3e-227
Identity = 405/531 (76.27%), Postives = 452/531 (85.12%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+  A VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGPPP S L+GNL EM+
Sbjct: 1   MGGLGSVLFAAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPPP-SLLYGNLQEMQ 60

Query: 66  EIELQ-AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           EI+L+ AAAM SPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN VD
Sbjct: 61  EIQLEAAAAMVSPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHVD 120

Query: 126 LVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSL+    L KP Y+ KK AP+LGH ++ +NG  W LQ KIIAPEFFMD+VR MA 
Sbjct: 121 LVKDLSLTNYLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDQVRVMAT 180

Query: 186 LMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSK 245
           LM EAAA ML KWE RIG EGEGA AEI+V+E LR FSADVISRACFGSSYEKGK IFSK
Sbjct: 181 LMTEAAAAMLQKWEGRIG-EGEGATAEIDVDEDLREFSADVISRACFGSSYEKGKRIFSK 240

Query: 246 LRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTSS 305
           LR LQKL+S GN LF           +S  RRIKKLEKEIESLIWETVQQRQ+E SKTSS
Sbjct: 241 LRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRRIKKLEKEIESLIWETVQQRQKECSKTSS 300

Query: 306 LEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALH 365
            +KDLLQ++ME AMNDPT+ A CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLALH
Sbjct: 301 -DKDLLQLIMEAAMNDPTVAANCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLALH 360

Query: 366 PEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGN 425
           PEWQDRIRSEF  AC DGR++ T ISQLKS+SMVVQETLRLYPP A+VARETF+ETRLGN
Sbjct: 361 PEWQDRIRSEFGQACLDGRVNMTTISQLKSVSMVVQETLRLYPPAAFVARETFSETRLGN 420

Query: 426 VVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCL 485
           VV+PKG+ LWTLIP LH EVEIWGE+ANEFKPERF  GIAKACKFPQAYLPFGAG R CL
Sbjct: 421 VVIPKGIYLWTLIPTLHREVEIWGENANEFKPERFVNGIAKACKFPQAYLPFGAGPRLCL 480

Query: 486 GKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           GKNFALVQLKI+ISL+VSKFRFSLSP+YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 GKNFALVQLKIVISLIVSKFRFSLSPEYRHSPSYRMIVEPANGMKIIFKRV 528

BLAST of Lag0041066 vs. ExPASy TrEMBL
Match: A0A6J1FRZ8 (cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447915 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.4e-226
Identity = 404/532 (75.94%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 65
           +G L S+    VA+AVVAV+VHFYYGVWRRTAE+RRKL AQGVTGP P S L+GNLPEM+
Sbjct: 1   MGGLGSVLFTAVAVAVVAVVVHFYYGVWRRTAEIRRKLRAQGVTGPSP-SLLYGNLPEMQ 60

Query: 66  EIELQ--AAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDV 125
           EI+L+  AAAMASPPHH S  VAHDY STLFPYFVKWRKQYGPLYTYSTGTRQHLYAN V
Sbjct: 61  EIQLEAAAAAMASPPHHASVIVAHDYTSTLFPYFVKWRKQYGPLYTYSTGTRQHLYANHV 120

Query: 126 DLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMA 185
           DLVKDLSL+ +  L KP Y+ KK AP+LGH ++ +NG  W LQ KIIAPEFFMDRVR MA
Sbjct: 121 DLVKDLSLTNHLHLGKPSYVTKKLAPILGHSIVRANGPVWALQRKIIAPEFFMDRVRVMA 180

Query: 186 ALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFS 245
           ALM EAAA ML KWE RI +EG+GA AEI+V+E LR FSADVISRACFGSSYEKGK IFS
Sbjct: 181 ALMTEAAAAMLQKWEERI-DEGDGATAEIDVDEALREFSADVISRACFGSSYEKGKRIFS 240

Query: 246 KLRDLQKLISHGNPLF-----------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTS 305
           KLR LQKL+S GN LF           +S  R IKKLEKEIESLIWETVQQRQ+E SKTS
Sbjct: 241 KLRVLQKLLSEGNFLFGYVSLSDRLFQQSKHRTIKKLEKEIESLIWETVQQRQKECSKTS 300

Query: 306 SLEKDLLQVLMEGAMNDPTIGA-CNESCKKFIVDNCKTMYFAGHESTAATATWALMLLAL 365
           S +KDLLQ++ME AMNDPT+G+ CNES KKFIVDNCK++YFAGHESTA  ATW+LMLLAL
Sbjct: 301 S-DKDLLQLIMEAAMNDPTVGSNCNESPKKFIVDNCKSIYFAGHESTAVAATWSLMLLAL 360

Query: 366 HPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLG 425
           HPEWQDRIRSEF  ACPDGR++ TAISQLKS SMVVQETLRLYPP A+VARETF+ETRLG
Sbjct: 361 HPEWQDRIRSEFTQACPDGRVNMTAISQLKSASMVVQETLRLYPPAAFVARETFSETRLG 420

Query: 426 NVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTC 485
           NVV+PKGV LWTLIP LH EVEIWGE+AN+FKPERF  GIAKACKFPQAY+PFGAG R C
Sbjct: 421 NVVIPKGVYLWTLIPTLHREVEIWGENANKFKPERFVNGIAKACKFPQAYVPFGAGPRLC 480

Query: 486 LGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           LGKNFALVQLKI+ISL+VSKFRFSLS +YRHSPS+R+IVEP +G+KI+F+++
Sbjct: 481 LGKNFALVQLKIVISLIVSKFRFSLSSEYRHSPSYRMIVEPANGMKIIFKRV 529

BLAST of Lag0041066 vs. ExPASy TrEMBL
Match: A0A5A7VH75 (Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00950 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.8e-219
Identity = 385/533 (72.23%), Postives = 444/533 (83.30%), Query Frame = 0

Query: 1   MEGALLGDLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG ++   V +FP  V MAVV +L+HFY YG WRRTA+VRRKL AQGVTGPPP S L+G
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPP-SILYG 60

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 61  NLPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 120

Query: 121 ANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N ++ KP Y+ +K AP+LG  V+ SNG+ W +Q K+IAPEFFMDRV
Sbjct: 121 ANKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRV 180

Query: 181 RGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R M   MV+AA+ +++KWE R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK
Sbjct: 181 RAMGFHMVDAASCLINKWERRVGD----GTVEIDVDEDLRGFSADVISRACFGSSYEKGK 240

Query: 241 HIFSKLRDLQKLISHGNPLFRSS---------FRRIKKLEKEIESLIWETVQQRQEESSK 300
            IFSKLRDLQKLIS  + LF  S          + IK+LEKEIES+IWETVQQRQ+E SK
Sbjct: 241 EIFSKLRDLQKLISEDSFLFGYSSWRFLRPRKHKSIKRLEKEIESIIWETVQQRQKECSK 300

Query: 301 TSSLEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLA 360
           TSS +KDLLQ++ME  +NDP IGA  +S K FIVDNCK++YFAGHESTA  ATW+LMLLA
Sbjct: 301 TSSSDKDLLQLIMEATINDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLA 360

Query: 361 LHPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRL 420
           LHPEWQDRIRSEFA ACPDG LDTTA  QLKS++MV+ ETLRLYPP A+VARETFAET+L
Sbjct: 361 LHPEWQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAETQL 420

Query: 421 GNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRT 480
           GNVVVPKGVCLWTLIPMLH EVEIWGEDAN+FKPERFA G+AKACKFPQAY+PFGAG R 
Sbjct: 421 GNVVVPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGPRL 480

Query: 481 CLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           CLGKNFALV+LKIIISL+VSKFRFSLSP+Y H PS+R++VEP +GVKIVFQ+L
Sbjct: 481 CLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 527

BLAST of Lag0041066 vs. ExPASy TrEMBL
Match: A0A1S3CNV5 (cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.9e-219
Identity = 385/535 (71.96%), Postives = 444/535 (82.99%), Query Frame = 0

Query: 1   MEGALLGDLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFG 60
           MEG ++   V +FP  V MAVV +L+HFY YG WRRTA+VRRKL AQGVTGPPP S L+G
Sbjct: 1   MEGYVISGFVLVFPTAVVMAVVMILLHFYFYGAWRRTADVRRKLRAQGVTGPPP-SILYG 60

Query: 61  NLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLY 120
           NLPEM++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLY
Sbjct: 61  NLPEMQKIQLQTAAMASPLHCASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLY 120

Query: 121 ANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRV 180
           AN V+LVKDLSLS N ++ KP Y+ +K AP+LG  V+ SNG+ W +Q K+IAPEFFMDRV
Sbjct: 121 ANKVELVKDLSLSNNLNVGKPFYVTRKLAPILGQSVVRSNGSVWAVQRKVIAPEFFMDRV 180

Query: 181 RGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGK 240
           R M   MV+AA+ +++KWE R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK
Sbjct: 181 RAMGFHMVDAASCLINKWERRVGD----GTVEIDVDEDLRGFSADVISRACFGSSYEKGK 240

Query: 241 HIFSKLRDLQKLISHGNPLFRSS-----------FRRIKKLEKEIESLIWETVQQRQEES 300
            IFSKLRDLQKLIS  + LF  S            + IK+LEKEIES+IWETVQQRQ+E 
Sbjct: 241 EIFSKLRDLQKLISEDSFLFGYSSWSDRFLRPRKHKSIKRLEKEIESIIWETVQQRQKEC 300

Query: 301 SKTSSLEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALML 360
           SKTSS +KDLLQ++ME  +NDP IGA  +S K FIVDNCK++YFAGHESTA  ATW+LML
Sbjct: 301 SKTSSSDKDLLQLIMEATINDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLML 360

Query: 361 LALHPEWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAET 420
           LALHPEWQDRIRSEFA ACPDG LDTTA  QLKS++MV+ ETLRLYPP A+VARETFAET
Sbjct: 361 LALHPEWQDRIRSEFAQACPDGHLDTTATLQLKSVNMVIHETLRLYPPAAFVARETFAET 420

Query: 421 RLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGH 480
           +LGNVVVPKGVCLWTLIPMLH EVEIWGEDAN+FKPERFA G+AKACKFPQAY+PFGAG 
Sbjct: 421 QLGNVVVPKGVCLWTLIPMLHREVEIWGEDANKFKPERFANGVAKACKFPQAYVPFGAGP 480

Query: 481 RTCLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           R CLGKNFALV+LKIIISL+VSKFRFSLSP+Y H PS+R++VEP +GVKIVFQ+L
Sbjct: 481 RLCLGKNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMVVEPANGVKIVFQRL 529

BLAST of Lag0041066 vs. ExPASy TrEMBL
Match: A0A0A0KQQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 8.3e-219
Identity = 386/530 (72.83%), Postives = 441/530 (83.21%), Query Frame = 0

Query: 6   LGDLVSIFPAGVAMAVVAVLVHFY-YGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEM 65
           +   V +FP  + +AVVA+L+HFY YG WRRTAEVRRKL AQGVTGPPP S L+GNLPEM
Sbjct: 1   MSGFVLVFPTAIVVAVVAILLHFYSYGAWRRTAEVRRKLEAQGVTGPPP-SILYGNLPEM 60

Query: 66  KEIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVD 125
           ++I+LQ AAMASP H  S  VAHDY STLFPYFV+WRKQYGPLYTYSTGTRQHLYAN V+
Sbjct: 61  QKIQLQTAAMASPLHRASAIVAHDYTSTLFPYFVEWRKQYGPLYTYSTGTRQHLYANKVE 120

Query: 126 LVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAA 185
           LVKDLSLS N ++ KP Y+ KK AP+LG  V+ SNGA W +Q K+IAPEFFMDRVR MA 
Sbjct: 121 LVKDLSLSNNLNVGKPFYVTKKLAPILGQSVVRSNGAVWAVQRKVIAPEFFMDRVRAMAF 180

Query: 186 LMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSK 245
            MV+AA+ +++KWE+R+G+       EI+V+E LRGFSADVISRACFGSSYEKGK IFSK
Sbjct: 181 HMVDAASCLINKWESRVGD----GTIEIDVDEDLRGFSADVISRACFGSSYEKGKEIFSK 240

Query: 246 LRDLQKLISHGNPLFRSS-----------FRRIKKLEKEIESLIWETVQQRQEESSKTSS 305
           LRDLQKLI   + LF  S            +RI +LEKEIESLIWETVQQRQ+E SKTSS
Sbjct: 241 LRDLQKLICEDSFLFGYSSWSDRFLQPRKHKRINRLEKEIESLIWETVQQRQKECSKTSS 300

Query: 306 LEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHP 365
            +KDLLQ++ME  ++DP IGA  +S K FIVDNCK++YFAGHESTA  ATW+LMLLALHP
Sbjct: 301 SDKDLLQLIMEATIDDPNIGA-KDSSKNFIVDNCKSIYFAGHESTAVAATWSLMLLALHP 360

Query: 366 EWQDRIRSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNV 425
           EWQDRIRSEFA ACPDG LD TA SQLKS+SMV+ ETLRLYPP A+VARETFAET+LGNV
Sbjct: 361 EWQDRIRSEFAQACPDGHLDPTATSQLKSVSMVIHETLRLYPPAAFVARETFAETQLGNV 420

Query: 426 VVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLG 485
           VVPKGVCLWTLIP LH EVEIWGEDANEFKPERFA G+AKACKFPQAY+PFGAG R CLG
Sbjct: 421 VVPKGVCLWTLIPTLHREVEIWGEDANEFKPERFANGVAKACKFPQAYVPFGAGPRLCLG 480

Query: 486 KNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           KNFALV+LKIIISL+VSKFRFSLSP+Y H PS+R+IVEP +GVKIVFQ+L
Sbjct: 481 KNFALVELKIIISLIVSKFRFSLSPEYHHCPSYRMIVEPANGVKIVFQRL 524

BLAST of Lag0041066 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 539.7 bits (1389), Expect = 2.7e-153
Identity = 275/519 (52.99%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALF-GNLPEMKEIELQAAAMA 76
           V + V+ + +  Y  V      +RRKL  QGV GPPPS  LF GN+PEM++I  Q+  M+
Sbjct: 16  VVIGVLGLGIRVYGKVMAEQWRMRRKLTMQGVKGPPPS--LFRGNVPEMQKI--QSQIMS 75

Query: 77  SPPHHPSPT-VAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKN 136
           +  H+     +AHDY S+LFPY   WRKQYG +YTYSTG +QHLY N  +LVK+L+ +  
Sbjct: 76  NSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQANT 135

Query: 137 PDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPML 196
            +L K  Y+ K+   +LG GVI SNG  W  Q +IIAPEFF+D+V+GM  L+VE+A PML
Sbjct: 136 LNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPML 195

Query: 197 DKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISH 256
            KWE  +  EGE    +I V+E LR  SADVISRACFGSS+ KGK IFSKLR LQK I+H
Sbjct: 196 SKWEEMMKREGE-MVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITH 255

Query: 257 GNPLF-------------RSSFRRIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQV 316
            N LF             +    +I +LE+ IESLIWETV++R+ E       +KDL+Q+
Sbjct: 256 NNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKEREREC--VGDHKKDLMQL 315

Query: 317 LMEGAMN--DPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRI 376
           ++EGA +  D  +    +S K F+VDNCK++YFAGHE++A   +W LMLLAL+P WQ RI
Sbjct: 316 ILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRI 375

Query: 377 RSEFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGV 436
           R E  L C +G  D  +IS LK+++MV+QETLRLYPP A+V+RE   +T+LGN+VVPKGV
Sbjct: 376 RDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGV 435

Query: 437 CLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALV 496
           C+WTLIP LH + EIWG DANEF PERF++G++KACK PQ+++PFG G R CLGKNF ++
Sbjct: 436 CIWTLIPTLHRDPEIWGADANEFNPERFSEGVSKACKHPQSFVPFGLGTRLCLGKNFGMM 495

Query: 497 QLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKI 519
           +LK+++SL+VS+F F+LSP Y+HSP  R++VEP  GV I
Sbjct: 496 ELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVI 527

BLAST of Lag0041066 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 503.1 bits (1294), Expect = 2.8e-142
Identity = 255/516 (49.42%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 17  VAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMAS 76
           V + + +V  H Y         +RR L  QGV GPPP S   GN+ EM+ I+ +A   + 
Sbjct: 16  VIVGIFSVGYHVYGRAVVEQWRMRRSLKLQGVKGPPP-SIFNGNVSEMQRIQSEAKHCSG 75

Query: 77  PPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPD 136
                   ++HDY S+LFP+F  WRKQYG +YTYSTG +QHLY N  ++VK+LS +   +
Sbjct: 76  -----DNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQTNTLN 135

Query: 137 LDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDK 196
           L +  +I K+  P+LG+G+I SNG  W  Q +IIA EF  D+++GM  LMVE+A PML+K
Sbjct: 136 LGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNK 195

Query: 197 WEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISHGN 256
           WE  +   GE    +I V+E L+  SADVI++ACFGSS+ KGK IFS +RDL   I+  +
Sbjct: 196 WEEMVKRGGE-MGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS 255

Query: 257 PLFR-SSFR------------RIKKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQVLM 316
            LFR + F              I  LE E+ES IWETV++R+ E   T   +KDL+Q+++
Sbjct: 256 VLFRFNGFTDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTH--KKDLMQLIL 315

Query: 317 EGAMNDPTIGACNESC-KKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRSE 376
           EGAM        ++S  ++F+VDNCK++YFAGH+STA + +W LMLLAL+P WQ +IR E
Sbjct: 316 EGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDE 375

Query: 377 FALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLW 436
              +C +G  D  +I  LK+++MV+QET+RLYPP   V RE   + RLG++VVPKGVC+W
Sbjct: 376 ILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIW 435

Query: 437 TLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLK 496
           TLIP LH + EIWG DAN+FKPERF++GI+KACK+PQ+Y+PFG G RTC+GKNF ++++K
Sbjct: 436 TLIPALHRDPEIWGPDANDFKPERFSEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVK 495

Query: 497 IIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKI 519
           +++SL+VSKF F+LSP Y+HSPSH+++VEP  GV I
Sbjct: 496 VLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVI 522

BLAST of Lag0041066 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 317.8 bits (813), Expect = 1.7e-86
Identity = 174/497 (35.01%), Postives = 281/497 (56.54%), Query Frame = 0

Query: 39  VRRKLGAQGVTGPPPSSALFGNLPEMKEIELQAAAMASPPHHPSPTVAHDYISTLFPYFV 98
           +++ +  QG+TGP P   L GN+ ++ ++   +A+      +   ++ H+ +  L P++V
Sbjct: 36  IKKFMERQGITGPKP-RLLTGNIIDISKMLSHSAS------NDCSSIHHNIVPRLLPHYV 95

Query: 99  KWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILS 158
            W KQYG  +    GT   L   + +++K+L    NP   K     +     +G G++++
Sbjct: 96  SWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMA 155

Query: 159 NGADWTLQHKIIAPEFFMDRVRGMAALMVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGL 218
           NG  W  Q  + AP F  DR++G A  MVE    M ++    +GE       E+E+ E +
Sbjct: 156 NGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKEVGE-------EVEIGEEM 215

Query: 219 RGFSADVISRACFGSSYEKGKHIFSKLRDLQKLISH--------GNPLFRSSF-RRIKKL 278
           R  +AD+ISR  FGSS +KGK +FS L  LQ+L +         G+    S + R IK L
Sbjct: 216 RRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKSL 275

Query: 279 EKEIESLIWETVQQRQE--ESSKTSSLEKDLLQVLMEGAMNDPTIGACNESCKKFIVDNC 338
           + E+E L+ E +  R++  E  ++SS   DLL +L+    ++      N    + I+D C
Sbjct: 276 KTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNK-----NNLNVQMIMDEC 335

Query: 339 KTMYFAGHESTAATATWALMLLALHPEWQDRIRSEFALAC-PDGRLDTTAISQLKSLSMV 398
           KT +F GHE+T+   TW LMLLA +P WQD +R E    C  DG      +S L SL+ V
Sbjct: 336 KTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKV 395

Query: 399 VQETLRLYPPGAYVARETFAETRLGNVVVPKGVCLWTLIPMLHTEVEIWGEDANEFKPER 458
           + E+LRLYPP   + R  F + +LG++++PKG+ +W  +  +H   E+WGEDANEF PER
Sbjct: 396 INESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPER 455

Query: 459 FAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQLKIIISLVVSKFRFSLSPDYRHSPSH 518
           F     ++    + ++PF AG R C+G+ FA+++ KII++++VSKF F++S +YRH+P  
Sbjct: 456 FT---TRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIV 510

Query: 519 RIIVEPDDGVKIVFQKL 524
            + ++P  GV++V + L
Sbjct: 516 VLTIKPKYGVQLVLKPL 510

BLAST of Lag0041066 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-85
Identity = 181/530 (34.15%), Postives = 297/530 (56.04%), Query Frame = 0

Query: 9   LVSIFPAGVAMAVVAVLVHFYYGV---WRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMK 68
           L++I  + + + V  +L   Y  +   W     +++ +  QGVTGP P   L GN+ E+ 
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKP-RPLTGNILEIS 61

Query: 69  EIELQAAAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDL 128
            +  Q+A+      H      HD +  L P++V W KQYG  +    GT   L   + +L
Sbjct: 62  AMVSQSASKDCDSIH------HDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETEL 121

Query: 129 VKDLSLSKNPDLDKPPYIFKKFAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAAL 188
           +K+L +  N    +     +     +G G++++NG DW  Q  + AP F  +R++G A  
Sbjct: 122 IKELLMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARH 181

Query: 189 MVEAAAPMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKL 248
           MVE  + ++++    +GE     A E+E+ E +   +AD+ISR  FGSS+EKGK +F+ L
Sbjct: 182 MVECTSKLVERLRKEVGE----GANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHL 241

Query: 249 RDLQK--------LISHGNPLFRSSF-RRIKKLEKEIESLIWETVQQRQE--ESSKTSSL 308
             LQ+        L   G+    S + R IK L+KE+E L+ E +Q R++  E  ++S+ 
Sbjct: 242 TVLQRRCAQATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTH 301

Query: 309 EKDLLQVLMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPE 368
             DLL +L+   M+       N +  + I+D CKT +FAGHE+TA   TW  MLLA +P 
Sbjct: 302 GDDLLGLLL-NEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPT 361

Query: 369 WQDRIRSEF-ALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNV 428
           WQ+++R E   +   +G      +S+L SLS V+ E+LRLYPP   + R  F + +LG++
Sbjct: 362 WQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDL 421

Query: 429 VVPKGVCLWTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLG 488
            +PKG+ +W  +  +H   E+WG+DAN+F PERF     +     + ++PF AG R C+G
Sbjct: 422 TIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG---GRPFASGRHFIPFAAGPRNCIG 481

Query: 489 KNFALVQLKIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           + FAL++ KII++ ++SKF F++S +YRH+P   + ++P  GV+++ + L
Sbjct: 482 QQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Lag0041066 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 306.2 bits (783), Expect = 5.0e-83
Identity = 167/522 (31.99%), Postives = 268/522 (51.34%), Query Frame = 0

Query: 12  IFPAGVAMAVVAVLVHFYYGVWRRTAEVRRKLGAQGVTGPPPSSALFGNLPEMKEIELQA 71
           IF     + +  + +  +   W      ++KL   G  GP PS   FGNL +MK++++  
Sbjct: 12  IFFKVFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFP-FGNLNDMKKLKM-- 71

Query: 72  AAMASPPHHPSPTVAHDYISTLFPYFVKWRKQYGPLYTYSTGTRQHLYANDVDLVKDLSL 131
           A++       S  + HD  S   P+F +W+++YG ++ Y  G    +Y  D + +  +S 
Sbjct: 72  ASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSK 131

Query: 132 SKNPDLDKPPYIFKK-FAPLLGHGVILSNGADWTLQHKIIAPEFFMDRVRGMAALMVEAA 191
                    P +FKK   P+ G G+++  G DWT    II P F    ++ M  +MVE+ 
Sbjct: 132 GVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESV 191

Query: 192 APMLDKWEARIGEEGEGAAAEIEVEEGLRGFSADVISRACFGSSYEKGKHIFSKLRDLQK 251
           + MLD+W  +I         E ++E  + G + ++I++  FG + E G  +   LR +Q 
Sbjct: 192 SNMLDRWGIQI----NSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQF 251

Query: 252 LISHGNPLFRSSFRRI---------KKLEKEIESLIWETVQQRQEESSKTSSLEKDLLQV 311
            + + N      F  I         K L  EI+ L+   + +R+   ++      DLL +
Sbjct: 252 ALFNSNRYVGVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGM 311

Query: 312 LMEGAMNDPTIGACNESCKKFIVDNCKTMYFAGHESTAATATWALMLLALHPEWQDRIRS 371
           L++                K +VD CKT +FAGHE+TA   TW  MLLA+HPEWQD IR 
Sbjct: 312 LLKADQK-------GNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIRE 371

Query: 372 EFALACPDGRLDTTAISQLKSLSMVVQETLRLYPPGAYVARETFAETRLGNVVVPKGVCL 431
           E      D +++   ++ LK +S V+ E LRLYPP     R+   +  +   V+P G  +
Sbjct: 372 EIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNI 431

Query: 432 WTLIPMLHTEVEIWGEDANEFKPERFAKGIAKACKFPQAYLPFGAGHRTCLGKNFALVQL 491
           W  +  +H +VE+WG+D NEFKPERF   +   CK    Y+PFG G R C+G+N   ++ 
Sbjct: 432 WIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEY 491

Query: 492 KIIISLVVSKFRFSLSPDYRHSPSHRIIVEPDDGVKIVFQKL 524
           KI++SLV+S+F  S+SP YRHSP++ + + P  G+ ++ + L
Sbjct: 492 KIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023516881.13.1e-22876.69cytochrome P450 714A1-like [Cucurbita pepo subsp. pepo][more]
KAG6599934.15.3e-22876.55Cytochrome P450 714A1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022995469.11.7e-22676.27cytochrome P450 714A1-like [Cucurbita maxima][more]
XP_022943066.12.9e-22675.94cytochrome P450 714A1-like isoform X1 [Cucurbita moschata] >XP_022943067.1 cytoc... [more]
XP_038893046.13.8e-22675.05cytochrome P450 714A1-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q93Z793.8e-15252.99Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1[more]
Q6NKZ83.9e-14149.42Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1[more]
Q2QYH73.6e-12645.99Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
Q5KQH73.8e-12043.52Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ... [more]
B6SSW81.1e-11644.31Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K5V58.3e-22776.27cytochrome P450 714A1-like OS=Cucurbita maxima OX=3661 GN=LOC111490994 PE=3 SV=1[more]
A0A6J1FRZ81.4e-22675.94cytochrome P450 714A1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
A0A5A7VH752.8e-21972.23Cytochrome P450 714A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CNV54.9e-21971.96cytochrome P450 714A1-like OS=Cucumis melo OX=3656 GN=LOC103502912 PE=3 SV=1[more]
A0A0A0KQQ78.3e-21972.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G224130 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G24910.12.7e-15352.99cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.12.8e-14249.42cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
AT1G67110.11.7e-8635.01cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G38450.11.4e-8534.15cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT5G52400.15.0e-8331.99cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 266..286
NoneNo IPR availablePANTHERPTHR24282:SF36CYTOCHROME P450 714A1-RELATEDcoord: 18..518
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 18..518
IPR001128Cytochrome P450PRINTSPR00385P450coord: 333..350
score: 37.17
coord: 471..482
score: 39.43
coord: 385..396
score: 44.24
coord: 462..471
score: 59.59
IPR001128Cytochrome P450PFAMPF00067p450coord: 93..504
e-value: 4.9E-71
score: 239.8
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 342..368
score: 25.82
coord: 322..339
score: 21.76
coord: 221..239
score: 23.31
coord: 461..471
score: 54.58
coord: 471..494
score: 24.29
coord: 101..120
score: 24.36
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..523
e-value: 5.7E-99
score: 333.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 77..521
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 464..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0041066.1Lag0041066.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen