Lag0040545 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040545 vs. NCBI nr
Match: XP_038891874.1 (F-box protein At3g54460 isoform X1 [Benincasa hispida])

HSP 1 Score: 2512.3 bits (6510), Expect = 0.0e+00
Identity = 1224/1370 (89.34%), Postives = 1286/1370 (93.87%), Query Frame = 0

Query: 1    MADDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSP 60
            M + + FSD++LCGFL VVLAVPSPQSEL N LRPGTRCYVS EGSDV FTS+NGV+LSP
Sbjct: 1    MDNGDDFSDYKLCGFLCVVLAVPSPQSELLNVLRPGTRCYVSAEGSDVSFTSENGVMLSP 60

Query: 61   IEINPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRM 120
            IE NP+ +S   V  QDSEQCRGTVGGEG G+ E+GD TPKR+VSARGSRSSRKKRTNRM
Sbjct: 61   IEPNPKSVSNPRVLPQDSEQCRGTVGGEGIGAVEIGDLTPKRQVSARGSRSSRKKRTNRM 120

Query: 121  GMVHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFP 180
            G+VHGSMSVV+QIHALV+HKCLKIDAQV FVDIG DE AR VLLVDV+LP+ELWSGWQFP
Sbjct: 121  GLVHGSMSVVYQIHALVVHKCLKIDAQVIFVDIGVDEDARAVLLVDVHLPVELWSGWQFP 180

Query: 181  RSKTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDS 240
            RSKTVAGALF+HLSCEWQER+SILVGKD+S+ AHM  KSVWNLAECHVH+CKLHNSSG S
Sbjct: 181  RSKTVAGALFKHLSCEWQERNSILVGKDHSQAAHMFRKSVWNLAECHVHSCKLHNSSGGS 240

Query: 241  PNKRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDL 300
            PN+RLFELHEIFRSLPSI  SS+ ++TRMQPEDDY QSGIWDISDDIL NILKALRPLDL
Sbjct: 241  PNRRLFELHEIFRSLPSILNSSEREHTRMQPEDDYFQSGIWDISDDILFNILKALRPLDL 300

Query: 301  IRVASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGF 360
            +RVASTCRHLRSLA SIMPCMKLKLYPHQQAAVEWML+RERN EV YHPLY PFSTEDGF
Sbjct: 301  VRVASTCRHLRSLAASIMPCMKLKLYPHQQAAVEWMLHRERNAEVFYHPLYAPFSTEDGF 360

Query: 361  SFHINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQII 420
            SFH+NTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+
Sbjct: 361  SFHVNTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIV 420

Query: 421  WCTHNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRY 480
            WCTHNGNRKCGYYEVSSNSNTS+NHFLVKEAV  NSLKGLE+LT H PKR R+  LDDR+
Sbjct: 421  WCTHNGNRKCGYYEVSSNSNTSTNHFLVKEAVAWNSLKGLEDLTYHTPKRARVTTLDDRH 480

Query: 481  TIDNDSCGGNELRSPSSADYAKAIHMVRCTRSL-SSVKRNLLFTNEGASSLSKELNAGKK 540
            T+ NDSC  NELRSPSSADYAKA+H+VRCTRSL SSVKRNLL   EGASSLSKELN GKK
Sbjct: 481  TVTNDSCAVNELRSPSSADYAKAVHVVRCTRSLSSSVKRNLLLAYEGASSLSKELNDGKK 540

Query: 541  STRTLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSI 600
            STRT TRKF AGAK+ G+SNGFTN+ E+P TT  DK EY DTWVQCDACHKWRKLAETSI
Sbjct: 541  STRTRTRKFPAGAKKVGVSNGFTNNYEIPWTTNADKLEYNDTWVQCDACHKWRKLAETSI 600

Query: 601  ADASAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLK 660
            AD+SAAWFCSMNT+PFYQSCSVPEESYDKCRPITNI GFY+KETSGGEEKNISFFTSVLK
Sbjct: 601  ADSSAAWFCSMNTNPFYQSCSVPEESYDKCRPITNILGFYTKETSGGEEKNISFFTSVLK 660

Query: 661  ENRALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGL 720
            ENRALINSGTKRALTWLSSL PEKVSEMER GLRSPILTSYVVPGG+ RGFHQIFEAFGL
Sbjct: 661  ENRALINSGTKRALTWLSSLAPEKVSEMERTGLRSPILTSYVVPGGNVRGFHQIFEAFGL 720

Query: 721  VRKIEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKT 780
            VRK+ KGT+RWYYP NLHNLAFDVAALR ALSEP+D VR YLSRATLIVVPSNLVDHWKT
Sbjct: 721  VRKMVKGTMRWYYPQNLHNLAFDVAALRIALSEPIDLVRLYLSRATLIVVPSNLVDHWKT 780

Query: 781  QIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHR 840
            QIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHR
Sbjct: 781  QIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHR 840

Query: 841  VILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEA 900
            VILDEGHTLGSSLNLTNKLQMA+SLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEA
Sbjct: 841  VILDEGHTLGSSLNLTNKLQMAVSLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEA 900

Query: 901  YGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTE 960
            YGQNHKSWEAGILRPFEAEMEEGR+LLLNLLRRCMISARKTDLL+IPPC+KKVKYL+FTE
Sbjct: 901  YGQNHKSWEAGILRPFEAEMEEGRLLLLNLLRRCMISARKTDLLTIPPCVKKVKYLNFTE 960

Query: 961  EHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVT 1020
            EHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKV 
Sbjct: 961  EHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVA 1020

Query: 1021 EAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLD 1080
            EAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGG+C+RCGEWCRLPVIAPCRHLLCLD
Sbjct: 1021 EAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGSCSRCGEWCRLPVIAPCRHLLCLD 1080

Query: 1081 CVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQS 1140
            CVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQS
Sbjct: 1081 CVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQS 1140

Query: 1141 TSSSKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLI 1200
            TSSSKV YLIQRLKALSEANDEAALVP P LSKSDALLQEVDHSR I S H I+ +KVLI
Sbjct: 1141 TSSSKVTYLIQRLKALSEANDEAALVPSPLLSKSDALLQEVDHSRPITSDHGIIRDKVLI 1200

Query: 1201 FSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGL 1260
            FSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGL
Sbjct: 1201 FSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGL 1260

Query: 1261 DLSFVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDE 1320
            DLSFVTYVFLMEPIWDRSMEEQVISRAHRMGA RPIHVETLVM ETIEEQMVQFLQDTDE
Sbjct: 1261 DLSFVTYVFLMEPIWDRSMEEQVISRAHRMGAIRPIHVETLVMHETIEEQMVQFLQDTDE 1320

Query: 1321 CKRLMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEKVSLD 1370
            CKRLM+EEFG  DYEGPRAHRSLHDFAGSNYLSQLKFVRTNP++EK  ++
Sbjct: 1321 CKRLMKEEFGKPDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPILEKGKME 1370

BLAST of Lag0040545 vs. NCBI nr
Match: KAG7031930.1 (F-box protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2505.3 bits (6492), Expect = 0.0e+00
Identity = 1214/1362 (89.13%), Postives = 1278/1362 (93.83%), Query Frame = 0

Query: 4    DEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIEI 63
            D+ FSD++LCGFL VVLAVPS QSELANALRPGTRCYVS E SDVCFTS+ GVVL+PIE 
Sbjct: 3    DDDFSDYKLCGFLCVVLAVPSRQSELANALRPGTRCYVSVESSDVCFTSEYGVVLTPIEA 62

Query: 64   NPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGMV 123
            NP+PLSK GVS QDSEQCRGTVGGEG+ + E GD T KR++SARG R+S KKRTNRMG+V
Sbjct: 63   NPKPLSKAGVSPQDSEQCRGTVGGEGTSTVENGDLTLKRKMSARGRRTSGKKRTNRMGLV 122

Query: 124  HGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRSK 183
            HGSMSVVHQIHALV+HKCL+IDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRSK
Sbjct: 123  HGSMSVVHQIHALVVHKCLRIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRSK 182

Query: 184  TVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPNK 243
            TVAGALFRHLSCEWQERSS+LVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N+
Sbjct: 183  TVAGALFRHLSCEWQERSSLLVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSNR 242

Query: 244  RLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIRV 303
            RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIRV
Sbjct: 243  RLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIRV 302

Query: 304  ASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSFH 363
            ASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML RERN EV YHPLYVPFSTEDGFSFH
Sbjct: 303  ASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLRRERNAEVFYHPLYVPFSTEDGFSFH 362

Query: 364  INTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWCT 423
            INTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WCT
Sbjct: 363  INTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWCT 422

Query: 424  HNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTID 483
            HNGNRKCGYYEVSSNSNTS+NH LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+ 
Sbjct: 423  HNGNRKCGYYEVSSNSNTSTNHLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTVT 482

Query: 484  NDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTRT 543
            NDSC GNE+ SPSS DYAKA+ MVRCTRSLSSVKRNLL T EGASSLS+ LN GKKSTRT
Sbjct: 483  NDSCAGNEMISPSSTDYAKAVQMVRCTRSLSSVKRNLLLTYEGASSLSRHLNTGKKSTRT 542

Query: 544  LTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADAS 603
             TRK AAGAKRAG+SNGFTN+ E+P TTT DKFEYKDTWVQCDACHKWRK +ETSIADAS
Sbjct: 543  RTRKLAAGAKRAGVSNGFTNNYEVPETTTADKFEYKDTWVQCDACHKWRKHSETSIADAS 602

Query: 604  AAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENRA 663
             AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFT VLKEN A
Sbjct: 603  TAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTGVLKENMA 662

Query: 664  LINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRKI 723
            LINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG DA GFH++FEAFGLVRK+
Sbjct: 663  LINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDACGFHKMFEAFGLVRKM 722

Query: 724  EKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQK 783
            EKGT+RWYYPHNLHNLAFDVAAL+TAL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQK
Sbjct: 723  EKGTIRWYYPHNLHNLAFDVAALKTALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQK 782

Query: 784  HVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVILD 843
            HVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHRVILD
Sbjct: 783  HVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHRVILD 842

Query: 844  EGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQN 903
            EGHTLGSSL+LTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEE YGQN
Sbjct: 843  EGHTLGSSLSLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEVYGQN 902

Query: 904  HKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHAR 963
            HKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHAR
Sbjct: 903  HKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHAR 962

Query: 964  SYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGE 1023
            SYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAGE
Sbjct: 963  SYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAGE 1022

Query: 1024 DIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVAL 1083
            DIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVAL
Sbjct: 1023 DIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVAL 1082

Query: 1084 DSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSS 1143
            D EGCTFPGCGKLYVMQTPET+ARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSS
Sbjct: 1083 DCEGCTFPGCGKLYVMQTPETIARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSS 1142

Query: 1144 KVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQF 1203
            KVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV EKVLIFSQF
Sbjct: 1143 KVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVREKVLIFSQF 1202

Query: 1204 LEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLSF 1263
            LEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSL  FQHDASCMVLLMDGSAALGLDLSF
Sbjct: 1203 LEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLATFQHDASCMVLLMDGSAALGLDLSF 1262

Query: 1264 VTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKRL 1323
            VTYVFLMEPIWD+SMEEQVISRAHRMGATRPIHVETLVM ETIEEQMVQFLQDTDECKRL
Sbjct: 1263 VTYVFLMEPIWDKSMEEQVISRAHRMGATRPIHVETLVMHETIEEQMVQFLQDTDECKRL 1322

Query: 1324 MREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            M+EEF   DYEGPRAHRS+HDFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1323 MKEEFSKPDYEGPRAHRSMHDFAGSNYLSQLKFVRTNPMMEK 1364

BLAST of Lag0040545 vs. NCBI nr
Match: KAG6601131.1 (F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2504.2 bits (6489), Expect = 0.0e+00
Identity = 1213/1363 (88.99%), Postives = 1278/1363 (93.76%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD+ FSD++LCGFL VVLAVPS QSELANALRPGTRCYVS E SDVCFTS+ GVVL+PIE
Sbjct: 2    DDDDFSDYKLCGFLCVVLAVPSRQSELANALRPGTRCYVSVESSDVCFTSEYGVVLTPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
             NP+PLSK GVS QDSEQCRGTVGGEG+ + E GD T KR++SARG R+S KKRTNRMG+
Sbjct: 62   ANPKPLSKAGVSPQDSEQCRGTVGGEGTSTVENGDLTLKRKMSARGRRTSGKKRTNRMGL 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            VHGSMSVVHQIHALV+HKCL+IDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRS
Sbjct: 122  VHGSMSVVHQIHALVVHKCLRIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSS+LVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N
Sbjct: 182  KTVAGALFRHLSCEWQERSSLLVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            +RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIR
Sbjct: 242  RRLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML RERN EV YHPLYVPFSTEDGFSF
Sbjct: 302  VASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLRRERNAEVFYHPLYVPFSTEDGFSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WC
Sbjct: 362  HINTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSSNSNTS+NH LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+
Sbjct: 422  THNGNRKCGYYEVSSNSNTSTNHLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTV 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             NDSC GNE+ SPSS DYAKA+ MVRCTRSLSSVKRNLL T EGASSLS+ LN GK+STR
Sbjct: 482  TNDSCAGNEMISPSSTDYAKAVQMVRCTRSLSSVKRNLLLTYEGASSLSRHLNTGKRSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRK AAGAKRAG+SNGFTN+ E+P TTT DKFEYKDTWVQCDACHKWRK +ETSIADA
Sbjct: 542  TRTRKLAAGAKRAGVSNGFTNNYEVPETTTADKFEYKDTWVQCDACHKWRKHSETSIADA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            S AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFT VLKEN 
Sbjct: 602  STAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTGVLKENM 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG DA GFH++FEAFGLVRK
Sbjct: 662  ALINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDACGFHKMFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAAL+TAL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALKTALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSL+LTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEE YGQ
Sbjct: 842  DEGHTLGSSLSLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEVYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            RSYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAG
Sbjct: 962  RSYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LD EGCTFPGCGKLYVMQTPET+ARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS
Sbjct: 1082 LDCEGCTFPGCGKLYVMQTPETIARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQLTIA IRFAGMYSPMHASNKMKSL  FQHDASCMVLLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLTIADIRFAGMYSPMHASNKMKSLATFQHDASCMVLLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVTYVFLMEPIWD+SMEEQVISRAHRMGATRPIHVETLVM ETIEEQMVQFLQDTDECKR
Sbjct: 1262 FVTYVFLMEPIWDKSMEEQVISRAHRMGATRPIHVETLVMHETIEEQMVQFLQDTDECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+EEF   DYEGPRAHRS+HDFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1322 LMKEEFSKPDYEGPRAHRSMHDFAGSNYLSQLKFVRTNPMMEK 1364

BLAST of Lag0040545 vs. NCBI nr
Match: XP_022956679.1 (F-box protein At3g54460 isoform X1 [Cucurbita moschata] >XP_022956680.1 F-box protein At3g54460 isoform X1 [Cucurbita moschata] >XP_022956681.1 F-box protein At3g54460 isoform X1 [Cucurbita moschata] >XP_022956682.1 F-box protein At3g54460 isoform X1 [Cucurbita moschata] >XP_022956683.1 F-box protein At3g54460 isoform X1 [Cucurbita moschata] >XP_022956684.1 F-box protein At3g54460 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2497.2 bits (6471), Expect = 0.0e+00
Identity = 1211/1363 (88.85%), Postives = 1274/1363 (93.47%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD+ FSD++LCGFL VVLAVPS QSEL NAL PGTRCYVS E SDVCFTS+ GVVL+PIE
Sbjct: 2    DDDDFSDYKLCGFLCVVLAVPSRQSELVNALCPGTRCYVSVESSDVCFTSEYGVVLTPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
             NP+PLSK GVS QDSEQC GTVGGEG+ + E GD T K ++SARG R+S KKRTNRMG+
Sbjct: 62   ANPKPLSKAGVSPQDSEQCGGTVGGEGTSTVENGDLTLKLKMSARGRRTSGKKRTNRMGL 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            VHGSMSVVHQIHALV+HKCLKIDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRS
Sbjct: 122  VHGSMSVVHQIHALVVHKCLKIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSS+LVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N
Sbjct: 182  KTVAGALFRHLSCEWQERSSLLVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            +RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIR
Sbjct: 242  RRLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML RERN EV YHPLYVPFSTEDGFSF
Sbjct: 302  VASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLRRERNTEVFYHPLYVPFSTEDGFSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WC
Sbjct: 362  HINTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSSNSNTS+NH LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+
Sbjct: 422  THNGNRKCGYYEVSSNSNTSTNHLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTV 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             NDSC GNE+ SPSS DYAKA+ MVRCTRSLSSV+RNLL T EGASSLS+ LN GKKSTR
Sbjct: 482  TNDSCAGNEMISPSSTDYAKAVQMVRCTRSLSSVERNLLLTYEGASSLSRHLNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRK AAGAKRAG+SNGFTN+ E+P TTT DKFEYKDTWVQCDACHKWRK +ETSIADA
Sbjct: 542  TRTRKLAAGAKRAGVSNGFTNNYEVPETTTADKFEYKDTWVQCDACHKWRKHSETSIADA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            S AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKEN 
Sbjct: 602  STAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENM 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG DA GFH++FEAFGLVRK
Sbjct: 662  ALINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDACGFHKMFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAAL+ AL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALKAALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEE YGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEVYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            RSYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAG
Sbjct: 962  RSYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LD EGCTFPGCGKLYVMQTPET+ARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS
Sbjct: 1082 LDCEGCTFPGCGKLYVMQTPETIARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQLTIAGIRFAGMYSPMHA NKMKSL  FQHDASCMVLLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLTIAGIRFAGMYSPMHACNKMKSLATFQHDASCMVLLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVTYVFLMEPIWD+SMEEQVISRAHRMGATRPIHVETLVM ETIEEQMVQFLQDTDECKR
Sbjct: 1262 FVTYVFLMEPIWDKSMEEQVISRAHRMGATRPIHVETLVMHETIEEQMVQFLQDTDECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+EEF   DYEGPRAHRS+HDFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1322 LMKEEFSKPDYEGPRAHRSMHDFAGSNYLSQLKFVRTNPMMEK 1364

BLAST of Lag0040545 vs. NCBI nr
Match: XP_023526902.1 (F-box protein At3g54460-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2496.1 bits (6468), Expect = 0.0e+00
Identity = 1209/1363 (88.70%), Postives = 1279/1363 (93.84%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD+ FSD++LCGFL VVLAVPSP SELA+ALRPGTRCYVS E SDVCFTS+ GVVL+PIE
Sbjct: 2    DDDDFSDYKLCGFLCVVLAVPSPLSELADALRPGTRCYVSVESSDVCFTSEYGVVLTPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
             NP+PLSK GVS QDSEQCRGTVGGEG+ + E GD T KR++SARG R+S KKRTNRMG+
Sbjct: 62   ANPKPLSKAGVSPQDSEQCRGTVGGEGTSTVENGDLTLKRKMSARGRRTSGKKRTNRMGL 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            VHGSMSVVHQIHALV+HKCLKIDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRS
Sbjct: 122  VHGSMSVVHQIHALVVHKCLKIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSS+LVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N
Sbjct: 182  KTVAGALFRHLSCEWQERSSLLVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            +RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIR
Sbjct: 242  RRLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML+RERN EV YHPLYVPFSTEDGFSF
Sbjct: 302  VASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLHRERNAEVFYHPLYVPFSTEDGFSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WC
Sbjct: 362  HINTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSSNSNTS+N+ LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+
Sbjct: 422  THNGNRKCGYYEVSSNSNTSTNNLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTV 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             NDSC GNE+ SPSS DYAKA+ MVRCTRSLSSVKRNLL T EGASSLS+ LN GKKSTR
Sbjct: 482  TNDSCAGNEMISPSSTDYAKAVQMVRCTRSLSSVKRNLLLTYEGASSLSRHLNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRK AAGAKRAG+SNGFTN+ E+  TTT DKFEYKDTWVQCDACHKWRKL+ETSIADA
Sbjct: 542  TRTRKLAAGAKRAGVSNGFTNNYEVSETTTADKFEYKDTWVQCDACHKWRKLSETSIADA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            S AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSK+TSGGEEKNISFFTSVLKEN 
Sbjct: 602  STAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKDTSGGEEKNISFFTSVLKENM 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG D  GFH++FEAFGLVRK
Sbjct: 662  ALINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDVCGFHKMFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAAL+TAL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALKTALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KH+RPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHIRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEEAYGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEAYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            RSYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAG
Sbjct: 962  RSYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LD EGCTFPGCGKLYVMQTPET+ARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS
Sbjct: 1082 LDCEGCTFPGCGKLYVMQTPETIARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV +KVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVRDKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIH++  QLTIAGIRFAGMYSPMHASNKMKSL  FQHDASCMVLLMDGSAALGLDLS
Sbjct: 1202 FLEHIHLL-AQLTIAGIRFAGMYSPMHASNKMKSLATFQHDASCMVLLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVTYVFLMEPIWD+SMEEQVISRAHRMGATRPIHVETLVM ETIEEQMVQFLQDTDECKR
Sbjct: 1262 FVTYVFLMEPIWDKSMEEQVISRAHRMGATRPIHVETLVMHETIEEQMVQFLQDTDECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+EEF   DYEGPRAHRS+HDFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1322 LMKEEFSKPDYEGPRAHRSMHDFAGSNYLSQLKFVRTNPMMEK 1363

BLAST of Lag0040545 vs. ExPASy Swiss-Prot
Match: Q9M1I1 (F-box protein At3g54460 OS=Arabidopsis thaliana OX=3702 GN=At3g54460 PE=2 SV=1)

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 798/1398 (57.08%), Postives = 990/1398 (70.82%), Query Frame = 0

Query: 9    DFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLS---PIEINP 68
            D +LCGFL  VL+V SP     + L+ G+ C++  +GS   F S+NG++LS   PI  N 
Sbjct: 4    DHKLCGFLCTVLSVDSP-----DLLQSGSSCFIFNDGSVTGFKSENGLILSLTNPIS-NL 63

Query: 69   EPL--SKGGVSRQDS-----------EQCRGTVGGEGSGSTEVGDFTPK---REVSARGS 128
            + L  SKG    ++S           ++ R  V GE SG  +    TPK   R +S    
Sbjct: 64   QSLISSKGDHDVENSGTIEDGRLETPQKRRKCVEGESSGKRK----TPKSKRRVLSGSKE 123

Query: 129  RSSR-KKRTNRMGMVHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVY 188
            ++ + +KR   +GMV+GS+SVV Q+HALV +KCLKI  +V  VD G + + R V+LVDVY
Sbjct: 124  KTVQGRKRVKSIGMVNGSISVVQQLHALVANKCLKIICRVVKVDKGENGEERAVVLVDVY 183

Query: 189  LPIELWSGWQFPRSKTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHV 248
            LPIELWSGWQFP+S+  A ALF+HLSC+W  R SIL GK    +A+   K++W+L++CHV
Sbjct: 184  LPIELWSGWQFPKSQATAAALFKHLSCDWGLRVSILDGKSIWEEANGRIKAIWDLSDCHV 243

Query: 249  HNCKLHNSSGDSPNKRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDIL 308
             +CKL  ++ +SP +RLF+LHEIF+SLPS         +R+ P  D   SG+WD+SDD+L
Sbjct: 244  FDCKLLCNAPNSPKRRLFKLHEIFKSLPSPGNHDVSYSSRVLPSTDSCVSGVWDLSDDVL 303

Query: 309  INILKALRPLDLIRVASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYH 368
            I+IL  L   DL  +A+ CR  RSL   I+PCM LKL+PHQQAAV WML RER  EV  H
Sbjct: 304  ISILMKLDTKDLFSIAAVCRLFRSLTSLIVPCMNLKLFPHQQAAVGWMLERERKAEVSSH 363

Query: 369  PLYVPFSTEDGFSFHINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQG 428
            PLY+ F TEDGFSF++N VTG+I+T  AP + DFRGG+FCDEPGLGKTITALSLILKTQG
Sbjct: 364  PLYLSFDTEDGFSFYVNAVTGDIITEAAPMVKDFRGGMFCDEPGLGKTITALSLILKTQG 423

Query: 429  TLAEPPAGVQIIWCTHNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKP 488
            T+A+PP G+ I+WCTH  ++KC YYE +S+  TS++   VK     +S +        +P
Sbjct: 424  TMADPPEGLPIVWCTHKSDKKCAYYEYTSDQFTSNSMSAVKRFQSPSSCRNQVSFEAFRP 483

Query: 489  ---------KRPRLMALDDRYTIDNDSCGGNELRS--PSSADYAKAIHMVRCTRSLSSVK 548
                     K+ RLM  DD+     +S   NE  +  P+S D        +C +SL +V+
Sbjct: 484  LLESKSLPFKQARLMDPDDQTLESKNSNFENEFETHIPASLDL-----KAQCRKSLGNVR 543

Query: 549  RNLLFTNEGASSLSKELNAGKKSTRTLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFE 608
            +NLL    GAS LS+ + A          K  +  K+ GM  G    C+  G T  D   
Sbjct: 544  KNLLPAYNGASELSEVMEA----------KRISNWKKCGMITG----CKRKGLTDSD--V 603

Query: 609  YKDTWVQCDACHKWRKLAETSIADASAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPG 668
              D W+QCD+C KWR++ +  ++   +AWFCS N DP YQSC+ PEE +DK +PI  + G
Sbjct: 604  ESDIWMQCDSCSKWRRIIDEGVSVTGSAWFCSNNNDPAYQSCNDPEELWDKSQPIKYLQG 663

Query: 669  FYSKETSGGEEKNISFFTSVLKENRALINSGTKRALTWLSSLTPEKVSEMERNGLRSPIL 728
            FY+K  SG E  NISFFTSVL+E+++ ++S  K+AL WL+ L  EK+S+ME  GL  P+L
Sbjct: 664  FYTKGASGEESDNISFFTSVLREHKSSVSSTVKKALIWLAKLPLEKLSQMETVGLPGPVL 723

Query: 729  TSYVVPGGDARGFHQIFEAFGLVRKIEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSV 788
               +    DA GF +IF AFGL  ++EKG  +W+YP  L NL FDV AL+ AL +PLD+ 
Sbjct: 724  GLKL----DALGFQRIFRAFGLKSRVEKGVTKWFYPKFLENLVFDVPALKVALCQPLDTF 783

Query: 789  RFYLSRATLIVVPSNLVDHWKTQIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTF 848
            R YLS+ATLIVVP+NLV+HW TQIQKHV   QL + VW DH + S H LAWDYDV+ITTF
Sbjct: 784  RLYLSKATLIVVPTNLVNHWLTQIQKHVCSDQLRILVWADHIELSPHSLAWDYDVVITTF 843

Query: 849  SRLSAEWGPRKKSILMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPT 908
            SRLSAEW PRKKS L+QVHW RV+LDEGHTLGSS++LTNK QMA+SL + NRW+LTGTPT
Sbjct: 844  SRLSAEWNPRKKSPLIQVHWLRVMLDEGHTLGSSVSLTNKFQMAVSLTACNRWLLTGTPT 903

Query: 909  PNTPNSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISA 968
            PNTPNSQLSH+QPLL+FLHEE YG+N K WEAGILRPFEAEMEEGR+ LL LL+RCMIS+
Sbjct: 904  PNTPNSQLSHIQPLLKFLHEEVYGENPKFWEAGILRPFEAEMEEGRLRLLQLLQRCMISS 963

Query: 969  RKTDLLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWK 1028
            RK DL  IPPCIKKV YL+F   HARSYNELV TVRRNIL+ADWNDPSHVESLLN KQWK
Sbjct: 964  RKKDLQMIPPCIKKVTYLNFLPGHARSYNELVETVRRNILLADWNDPSHVESLLNSKQWK 1023

Query: 1029 FRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNC 1088
            FRS TI N+RLSCCVAGHIK+T+AG DI+ETMD L+++ LD  ++EYSFI+ +L+ G NC
Sbjct: 1024 FRSITISNVRLSCCVAGHIKMTDAGHDIKETMDALLENDLDLWTEEYSFIQDSLIGGCNC 1083

Query: 1089 ARCGEWCRLPVIAPCRHLLCLDCVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPV 1148
             RCGEWCRLPVI PCRHLLCLDCVALDSE CT  GCG LY MQTPETLARPENPNPKWPV
Sbjct: 1084 KRCGEWCRLPVITPCRHLLCLDCVALDSERCTISGCGYLYEMQTPETLARPENPNPKWPV 1143

Query: 1149 PKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLKALSEANDEAALV-----------P 1208
            PKDLIELQPSYKQD+W+PDWQSTSSSKV+YL+ RL+ L E N ++ L             
Sbjct: 1144 PKDLIELQPSYKQDDWNPDWQSTSSSKVSYLVDRLRKLHEGNKKSILSFNKTDNDNLEDN 1203

Query: 1209 PPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHVIEQQLTIAGIRFAGMYS 1268
            PP    S+A L +  H +   S   + ++KVLIFSQFLEHIHVIEQQLT AGI+F  MYS
Sbjct: 1204 PP--GTSEAFLGKELHGQDCGS-QMVFVDKVLIFSQFLEHIHVIEQQLTTAGIKFGKMYS 1263

Query: 1269 PMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLSFVTYVFLMEPIWDRSMEEQVISRA 1328
            PM + NKMK+L MFQ+DA CM LLMDGS ALGLDLSFVT+VFLMEPIWD+S+EEQVISRA
Sbjct: 1264 PMQSYNKMKALAMFQNDADCMALLMDGSGALGLDLSFVTHVFLMEPIWDKSLEEQVISRA 1323

Query: 1329 HRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKRLMREEFGNLDYEGPRAHRSLHDFA 1365
            HRMGA RPI VETL MR TIEEQM++FL+D ++  RL+  ++     E  R+ R+LHD  
Sbjct: 1324 HRMGAKRPIFVETLTMRGTIEEQMMRFLEDAEKSDRLLSGDYIEAKQETTRSRRTLHDLV 1363

BLAST of Lag0040545 vs. ExPASy Swiss-Prot
Match: Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.6e-38
Identity = 150/582 (25.77%), Postives = 241/582 (41.41%), Query Frame = 0

Query: 766  LIVVPSNLVDHWKTQIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWG 825
            LIV P  L+  WKT+I+ H +PG L VYV     +P    L    DV+ITT+  L++E+ 
Sbjct: 493  LIVCPMTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLSQSDVVITTYGVLTSEFS 552

Query: 826  PRKKSI---LMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPN 885
                +    +  V W R++LDE HT+ +S +  +    A +LV+  RW LTGTP  N   
Sbjct: 553  QENSADHEGIYAVRWFRIVLDEAHTIKNSKSQIS--LAAAALVADRRWCLTGTPIQN--- 612

Query: 886  SQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTD- 945
              L  L  LLRFL  E +G     W   + +PFE   E G  L+ ++L+  M+   K+  
Sbjct: 613  -NLEDLYSLLRFLRIEPWG-TWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSST 672

Query: 946  ------LLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMAD---------WNDPSH 1005
                  +L +PP   +V Y   +E     Y+ L    +R+ +  D          N  S 
Sbjct: 673  DREGRPILVLPPADARVIYCELSESERDFYDAL---FKRSKVKFDQFVEQGKVLHNYASI 732

Query: 1006 VESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDI--------------L 1065
            +E LL  +Q              CC   H  +  +  D  E  D+              L
Sbjct: 733  LELLLRLRQ--------------CC--DHPFLVMSRGDTAEYSDLNKLSKRFLSGKSSGL 792

Query: 1066 VDDGLDPMSQEY-SFIKYNLLYG--GNCARCGEWCRLPVIAPCRHLLCLDCVALDSEGCT 1125
              +G D  S+ +   +   L  G  G C  C E     V+ PC H LC +C+       T
Sbjct: 793  EREGKDVPSEAFVQEVVEELRKGEQGECPICLEALEDAVLTPCAHRLCRECLLASWRNST 852

Query: 1126 FPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLI 1185
               C                 P  +  V K  +   P+  +   D +     SSK+  L+
Sbjct: 853  SGLC-----------------PVCRNTVSKQELITAPTESRFQVDVEKNWVESSKITALL 912

Query: 1186 QRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHV 1245
            + L+ L  +                                     K ++FSQ+   + +
Sbjct: 913  EELEGLRSSG-----------------------------------SKSILFSQWTAFLDL 972

Query: 1246 IEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSA-ALGLDLSFVTYVF 1305
            ++  L+     F  +   +    + K L  F  D S +VLLM   A  +G++L+  +  F
Sbjct: 973  LQIPLSRNNFSFVRLDGTLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAF 996

Query: 1306 LMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQM 1311
            +M+P W+ ++EEQ + R HR+G T+ + +   +++ T+EE+M
Sbjct: 1033 VMDPWWNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERM 996

BLAST of Lag0040545 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 2.9e-34
Identity = 159/580 (27.41%), Postives = 241/580 (41.55%), Query Frame = 0

Query: 765  TLIVVPSNLVDHWKTQIQKHVRPG--QLLVYVWTDHRKPSAHCLAWDYDVIITTFSRL-S 824
            TLI+ P  L+  WK +++ H +P    +LVY   D R   A  +A  +DV++TT+  L S
Sbjct: 743  TLIICPMALLSQWKDELETHSKPDTVSVLVYYGGD-RTHDAKAIA-SHDVVLTTYGVLTS 802

Query: 825  AEWGPRKKSILMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTP 884
            A       SI  ++ W+R++LDE HT+ S    T   +    L S  RW LTGTP  N  
Sbjct: 803  AYKQDMANSIFHRIDWYRIVLDEAHTIKSW--KTQAAKATFELSSHCRWCLTGTPLQN-- 862

Query: 885  NSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTD 944
              +L  L  LL FLH E +  N   W   I +P+E     G  L+  +LR  M+   K  
Sbjct: 863  --KLEDLYSLLCFLHVEPW-CNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKET 922

Query: 945  -------LLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMAD---------WNDPS 1004
                   +L +PP   +V     +E     Y  L    +R+ +  D          N  +
Sbjct: 923  RDKEGSLILELPPTDVQVIECEQSEAERDFYTAL---FKRSKVQFDQFVAQGKVLHNYAN 982

Query: 1005 HVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDIL----VDDGLDPMS 1064
             +E LL  +Q              CC    + ++ A       +D L    +D+  D +S
Sbjct: 983  ILELLLRLRQ--------------CCNHPFLVMSRADSQQYADLDSLARRFLDNNPDSVS 1042

Query: 1065 QEYSFIKY-----NLLYGGN---CARCGEWCRLPVIAPCRHLLCLDCVALDSEGCTFPGC 1124
            Q      Y       L  GN   C  C E    PV+ PC H +C +C+         P C
Sbjct: 1043 QNAPSRAYIEEVIQDLRDGNSKECPICLESADDPVLTPCAHRMCRECLLTSWRS---PSC 1102

Query: 1125 GKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLK 1184
            G   + +T   L R E  +     P D I      K  NW        SSKV+ L++ L+
Sbjct: 1103 GLCPICRT--ILKRTELIS----CPTDSIFRVDVVK--NW------KESSKVSELLKCLE 1162

Query: 1185 ALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHVIEQQ 1244
             + ++                                    EK ++FSQ+   + ++E  
Sbjct: 1163 KIKKSGSG---------------------------------EKSIVFSQWTSFLDLLEIP 1222

Query: 1245 LTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSA-ALGLDLSFVTYVFLMEP 1304
            L   G  F      +    + K L  F       +LLM   A  +GL+L+  + VFLM+P
Sbjct: 1223 LRRRGFEFLRFDGKLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLTAASSVFLMDP 1246

Query: 1305 IWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQ 1313
             W+ ++EEQ I R HR+G  R + V   ++++T+EE+M Q
Sbjct: 1283 WWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQ 1246

BLAST of Lag0040545 vs. ExPASy Swiss-Prot
Match: O13762 (Uncharacterized ATP-dependent helicase C17A2.12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17A2.12 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.1e-32
Identity = 144/601 (23.96%), Postives = 258/601 (42.93%), Query Frame = 0

Query: 761  LSRATLIVVPSNLVDHWKTQIQKHVRPG-QLLVYVWTDHRKPSAHCLAW-DYDVIITTFS 820
            L +  LIVV   L+  W  ++   V P  +L VY+     K +        YDV++TT+S
Sbjct: 299  LRKTNLIVVSVALLHQWAEELSTKVHPSKKLSVYIHHGSTKKNLDSYELSQYDVVLTTYS 358

Query: 821  RLSAEW--------------GPRKKSILMQVHWHRVILDEGHTLGSSLNLTNKLQMAISL 880
             L+ E                P     L++  W+R++LDE HT+ +   L  K    + L
Sbjct: 359  MLAYEMKQNDAFNNNNPATATPPPACSLLETSWYRIVLDEAHTIRNRDTLAAK--CCVKL 418

Query: 881  VSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAY----------GQNHKSWEAGILRP 940
             +  RW L+GTP  N     +     LL+FL  + Y           +  KS+ A I+  
Sbjct: 419  DAKYRWCLSGTPIQN----HIDEFYSLLKFLRIKPYCVWSLFAKDISRPLKSYRADIV-- 478

Query: 941  FEAEMEEGRILLLNLLRRCMISARKTDL--LSIPPCIKKVKYLHFTEEHARSYNELVVTV 1000
             EA ++  RILL + + R     R  +L  +++PP   +   ++   E    YNE + + 
Sbjct: 479  -EAALKRLRILLASTVFRRTKETRVNNLPIVNLPPKTIRTVSVNLLPEERALYNEQMSSA 538

Query: 1001 RRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGED--IQETMD 1060
            +   L+ ++ +  H     +  ++ F   ++  +R  CC    +K +       I+++ +
Sbjct: 539  QS--LVDNYFNNDH-----DLSRYGFLLVSLLRLRQFCCHPWLVKSSSLDNSFRIRDSEN 598

Query: 1061 IL-VDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPV-IAPCRHLLCLDCVAL----- 1120
            +      LDP++ E    +   L   NC+ C + C  PV I PC H  C +C+++     
Sbjct: 599  VRNACKSLDPLTIE----RIATLQDFNCSVCLDPCLAPVFIIPCGHFTCQECMSMLVGQK 658

Query: 1121 --DSEGCTF----PGC-GKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQD----- 1180
               S   T     P C G +      +         P   + +  +++  S+ +      
Sbjct: 659  YGSSSTSTIIAKCPMCRGNIVQDSLVDATILQAIHGPLNSLKQLELDMNQSFSEQESIKL 718

Query: 1181 NWDPDWQSTSSSKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHE 1240
             W+       + K        K+ S+ N     +     SK +                 
Sbjct: 719  RWENRIDQMFTKKFGKRASEWKSSSKLNQARQTILDIIGSKRN----------------- 778

Query: 1241 IVIEKVLIFSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLM 1300
               EK+L++SQF +++ ++   L +  IR       M A+ + KSL  F +D   +V+L+
Sbjct: 779  ---EKILVYSQFSQYLCLVSHMLKLENIRHVRYDGTMSANQRQKSLHSFNNDKDVLVMLV 838

Query: 1301 DGSA-ALGLDLSFVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQM 1312
               A ++GL+L+   +V L EP ++ S+E+Q I R HR+G  +P+ V   + ++TIEE++
Sbjct: 839  SLKAGSVGLNLTIANHVILQEPFYNPSIEDQAIDRVHRLGQQKPVTVYRFITKDTIEERI 859

BLAST of Lag0040545 vs. ExPASy Swiss-Prot
Match: P36607 (DNA repair protein rad8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rad8 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 6.7e-31
Identity = 138/576 (23.96%), Postives = 252/576 (43.75%), Query Frame = 0

Query: 762  SRATLIVVPSNLVDHWKTQIQKHVRPGQLLVYVWTDHRKP--SAHCLAWDYD---VIITT 821
            SR TL+V P +L+D W ++  K  +  +    ++    KP     C+        +IIT+
Sbjct: 569  SRTTLVVAPMSLLDQWHSEACKVSQGTKFRSMIYYGSEKPLDLKSCVIDTSTAPLIIITS 628

Query: 822  FSRLSAEWGPRKKSI-LMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGT 881
            +  L +E+  +  S  L  VHW RV+LDEGH + +  + T K   +IS  S NRW++TGT
Sbjct: 629  YGVLLSEFSQQSHSSGLFSVHWFRVVLDEGHNIRNRESKTAKACHSIS--SQNRWVITGT 688

Query: 882  PTPNTPNSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMI 941
            P  N    +L  L  L++F+  E +  N+  W+  +  P++++     + ++  +   ++
Sbjct: 689  PIVN----KLDDLYSLIKFMRYEPW-CNYTYWQTFVSLPYQSKDVLKALNVVQSILEFLV 748

Query: 942  SARKTD--------LLSIPPCIKKVKYLHFTEEHARSYNELVV----TVRRNILMADW-- 1001
              R  +        ++++PP   K++YL F++   + Y+ L      TV  NI+      
Sbjct: 749  LRRTKETKDRNGNSIVTLPPKTVKIEYLDFSDSERKIYDSLYTKAKSTVNANIVAGTLFR 808

Query: 1002 NDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDILVDDGLDPMS 1061
            N  + +  LL  +Q       + N+ ++       + +   E     ++  V  G  P+ 
Sbjct: 809  NYTTILGLLLRLRQACCDPVLLSNMTINSETFDDFEFSV--EQFNSLINQFVVTG-KPIP 868

Query: 1062 QEYSFIKYNLLYGGNCARCGEWCRLPVIAP----CRHLLCLDCVALDSEGCTFPGCGKLY 1121
             +   I     +      C   C  P+  P    C+H  C DC++               
Sbjct: 869  SDILKIDTLKSFEALITECPICCNEPIQNPLLLNCKHACCGDCLS--------------- 928

Query: 1122 VMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLKALSE 1181
                 E +   +  N   P+       QP  +QD + P +   + ++   L+        
Sbjct: 929  -----EHIQYQKRRNIIPPLCHTC--RQPFNEQDVYKPFFVKNNGTQSTLLVGEEVKWKY 988

Query: 1182 ANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHVIEQQLTIA 1241
             N   ++       K + LL ++         H    EKV+IFSQF   + +I   L   
Sbjct: 989  WNRLQSV-------KLNGLLGQLRQLT-----HSSEPEKVVIFSQFTTFLDIIADVLESE 1048

Query: 1242 GIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSA-ALGLDLSFVTYVFLMEPIWDR 1301
             + +A     M    +  +L  F++D    VL++   A  +GL+L+   +VF+M+P W  
Sbjct: 1049 KMGYARFDGTMSQQMRSTALETFRNDPDVNVLIISLKAGGVGLNLTCANHVFIMDPWWSW 1100

Query: 1302 SMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQ 1313
            S+E Q I R HR+G  +P+ V   ++R+T+EE+M++
Sbjct: 1109 SVEAQAIDRIHRLGQEKPVFVTRYIVRDTVEERMLK 1100

BLAST of Lag0040545 vs. ExPASy TrEMBL
Match: A0A6J1GZS0 (F-box protein At3g54460 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458330 PE=4 SV=1)

HSP 1 Score: 2497.2 bits (6471), Expect = 0.0e+00
Identity = 1211/1363 (88.85%), Postives = 1274/1363 (93.47%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD+ FSD++LCGFL VVLAVPS QSEL NAL PGTRCYVS E SDVCFTS+ GVVL+PIE
Sbjct: 2    DDDDFSDYKLCGFLCVVLAVPSRQSELVNALCPGTRCYVSVESSDVCFTSEYGVVLTPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
             NP+PLSK GVS QDSEQC GTVGGEG+ + E GD T K ++SARG R+S KKRTNRMG+
Sbjct: 62   ANPKPLSKAGVSPQDSEQCGGTVGGEGTSTVENGDLTLKLKMSARGRRTSGKKRTNRMGL 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            VHGSMSVVHQIHALV+HKCLKIDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRS
Sbjct: 122  VHGSMSVVHQIHALVVHKCLKIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSS+LVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N
Sbjct: 182  KTVAGALFRHLSCEWQERSSLLVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            +RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIR
Sbjct: 242  RRLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML RERN EV YHPLYVPFSTEDGFSF
Sbjct: 302  VASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLRRERNTEVFYHPLYVPFSTEDGFSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WC
Sbjct: 362  HINTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSSNSNTS+NH LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+
Sbjct: 422  THNGNRKCGYYEVSSNSNTSTNHLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTV 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             NDSC GNE+ SPSS DYAKA+ MVRCTRSLSSV+RNLL T EGASSLS+ LN GKKSTR
Sbjct: 482  TNDSCAGNEMISPSSTDYAKAVQMVRCTRSLSSVERNLLLTYEGASSLSRHLNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRK AAGAKRAG+SNGFTN+ E+P TTT DKFEYKDTWVQCDACHKWRK +ETSIADA
Sbjct: 542  TRTRKLAAGAKRAGVSNGFTNNYEVPETTTADKFEYKDTWVQCDACHKWRKHSETSIADA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            S AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKEN 
Sbjct: 602  STAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENM 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG DA GFH++FEAFGLVRK
Sbjct: 662  ALINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDACGFHKMFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAAL+ AL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALKAALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEE YGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEVYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            RSYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAG
Sbjct: 962  RSYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LD EGCTFPGCGKLYVMQTPET+ARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS
Sbjct: 1082 LDCEGCTFPGCGKLYVMQTPETIARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQLTIAGIRFAGMYSPMHA NKMKSL  FQHDASCMVLLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLTIAGIRFAGMYSPMHACNKMKSLATFQHDASCMVLLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVTYVFLMEPIWD+SMEEQVISRAHRMGATRPIHVETLVM ETIEEQMVQFLQDTDECKR
Sbjct: 1262 FVTYVFLMEPIWDKSMEEQVISRAHRMGATRPIHVETLVMHETIEEQMVQFLQDTDECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+EEF   DYEGPRAHRS+HDFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1322 LMKEEFSKPDYEGPRAHRSMHDFAGSNYLSQLKFVRTNPMMEK 1364

BLAST of Lag0040545 vs. ExPASy TrEMBL
Match: A0A6J1CCT9 (F-box protein At3g54460 OS=Momordica charantia OX=3673 GN=LOC111010048 PE=4 SV=1)

HSP 1 Score: 2488.0 bits (6447), Expect = 0.0e+00
Identity = 1222/1368 (89.33%), Postives = 1277/1368 (93.35%), Query Frame = 0

Query: 1    MADDE-HFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLS 60
            MADD   FSD++LCGFL VVLAVPSPQ +L+N LRPGTRCYVS EGS+VCFTS NGVVLS
Sbjct: 1    MADDSIPFSDYKLCGFLCVVLAVPSPQPDLSNILRPGTRCYVSAEGSEVCFTSGNGVVLS 60

Query: 61   PIEINPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNR 120
            PIE N +PLSK GVS QDSEQCRG VG   +GSTEV D TPK EVSARGSRSSR KR NR
Sbjct: 61   PIEENSKPLSKAGVSHQDSEQCRGPVGEGETGSTEVVDLTPKAEVSARGSRSSR-KRMNR 120

Query: 121  MGMVHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQF 180
            MG+VHGSMSVVHQIHALV+HKCLKIDA+V FVD+G D +AR VLLVDV+LP+ELWSGWQF
Sbjct: 121  MGLVHGSMSVVHQIHALVVHKCLKIDARVVFVDVGVDGEARAVLLVDVHLPVELWSGWQF 180

Query: 181  PRSKTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGD 240
            PRSK VAGALFRHLSCEWQERSSIL GKDYSRDA M  KS+WNLAECHVH CKLH+SSG 
Sbjct: 181  PRSKMVAGALFRHLSCEWQERSSILGGKDYSRDAPMVRKSMWNLAECHVHACKLHDSSGG 240

Query: 241  SPNKRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLD 300
            SPN+RLFELHEIFRSLPS+ KSSKPDYTRMQPEDD SQ GIWDISDD LINILKALRPLD
Sbjct: 241  SPNRRLFELHEIFRSLPSVVKSSKPDYTRMQPEDDSSQLGIWDISDDTLINILKALRPLD 300

Query: 301  LIRVASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDG 360
            LIRVASTCRHL+SLAVSIMPCMKLKLYPHQQAAVEWML+RERN EV YHPLYV F+TEDG
Sbjct: 301  LIRVASTCRHLKSLAVSIMPCMKLKLYPHQQAAVEWMLHRERNAEVFYHPLYVAFTTEDG 360

Query: 361  FSFHINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQI 420
            FSFHINTVTGEIV+GGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQI
Sbjct: 361  FSFHINTVTGEIVSGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQI 420

Query: 421  IWCTHNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDR 480
            IWC HNGNRKCGYYE+S N+NTSSNHFLV  AVGC+SLK LE+L  H PKR RL ALDDR
Sbjct: 421  IWCAHNGNRKCGYYELSGNNNTSSNHFLVNGAVGCSSLKALEDLKYHTPKRARLTALDDR 480

Query: 481  YTIDNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKK 540
            +T+ NDSC GNE+RSPSSADY KA+ MVRCTRSLSSVK+NLLFT EGA SLSKELNAG+K
Sbjct: 481  HTVINDSCAGNEMRSPSSADYEKAVDMVRCTRSLSSVKKNLLFTYEGAPSLSKELNAGRK 540

Query: 541  STRTLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSI 600
            STRT TRK AAGAKRAG+SNGFT++ E+PG  T DKFEYKDTWVQCDACHKWRKLAET +
Sbjct: 541  STRTWTRKVAAGAKRAGVSNGFTDNYEVPGMATADKFEYKDTWVQCDACHKWRKLAETCV 600

Query: 601  ADASAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLK 660
            ADASAAWFCSMN DPFYQSCSVPEESYDKCRPITNIPGFYSK TSGGEEKNISFFTSVLK
Sbjct: 601  ADASAAWFCSMNIDPFYQSCSVPEESYDKCRPITNIPGFYSKGTSGGEEKNISFFTSVLK 660

Query: 661  ENRALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGL 720
            EN ALINSGTKRAL WLSSLTPEKVSEMER GLR PILTSYVV GGDARGFH++FEAFGL
Sbjct: 661  ENSALINSGTKRALAWLSSLTPEKVSEMERTGLRCPILTSYVVSGGDARGFHKMFEAFGL 720

Query: 721  VRKIEKGTVRWYYPHNLHNLAFDVAALRTALSEPL-DSVRFYLSRATLIVVPSNLVDHWK 780
            VRK+EKG +RWYYPHNLHNLAFDVAALRTAL EPL +SVR YLSRATLIVVPSNLVDHWK
Sbjct: 721  VRKMEKGIIRWYYPHNLHNLAFDVAALRTALCEPLVNSVRLYLSRATLIVVPSNLVDHWK 780

Query: 781  TQIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWH 840
            TQIQKHVRPGQLLVYVWTDH+KPSAHCLAWDYDVIITTFSRLSAEWGPRK+SILMQVHW 
Sbjct: 781  TQIQKHVRPGQLLVYVWTDHKKPSAHCLAWDYDVIITTFSRLSAEWGPRKRSILMQVHWL 840

Query: 841  RVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEE 900
            RVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEE
Sbjct: 841  RVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEE 900

Query: 901  AYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFT 960
            AYGQNHKSWEAGILRPFEAEMEEGR+LLLNLLRRCM+SARKTDLLSIPPCIKKVKYL+FT
Sbjct: 901  AYGQNHKSWEAGILRPFEAEMEEGRLLLLNLLRRCMVSARKTDLLSIPPCIKKVKYLNFT 960

Query: 961  EEHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKV 1020
            EEHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKV
Sbjct: 961  EEHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKV 1020

Query: 1021 TEAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCL 1080
            TEAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCL
Sbjct: 1021 TEAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCL 1080

Query: 1081 DCVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQ 1140
             CVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQD+WDPDWQ
Sbjct: 1081 GCVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDDWDPDWQ 1140

Query: 1141 STSSSKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVL 1200
            STSSSKVAYLIQRLKALSE NDEA +VPPPSL+KS  LLQEVDHSR   S HEIV EKVL
Sbjct: 1141 STSSSKVAYLIQRLKALSEVNDEATVVPPPSLNKSGGLLQEVDHSRTTTSDHEIVREKVL 1200

Query: 1201 IFSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALG 1260
            IFSQFLEHIHVIEQQLTIAGIRFAGMYSPMHA NKMKSLTMFQHD+SCMVLLMDGSAALG
Sbjct: 1201 IFSQFLEHIHVIEQQLTIAGIRFAGMYSPMHACNKMKSLTMFQHDSSCMVLLMDGSAALG 1260

Query: 1261 LDLSFVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTD 1320
            LDLSFVT+VFLMEPIWDRSMEEQVISRAHRMGA RPIHVETLVMRETIEEQMVQFLQDTD
Sbjct: 1261 LDLSFVTHVFLMEPIWDRSMEEQVISRAHRMGAIRPIHVETLVMRETIEEQMVQFLQDTD 1320

Query: 1321 ECKRLMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEKV 1367
            ECKRL++EEF   DYEGPRAHRSLHDFAGSNYLSQLKFVRTN  +EKV
Sbjct: 1321 ECKRLLKEEFIKPDYEGPRAHRSLHDFAGSNYLSQLKFVRTNAKVEKV 1367

BLAST of Lag0040545 vs. ExPASy TrEMBL
Match: A0A6J1KT32 (LOW QUALITY PROTEIN: F-box protein At3g54460-like OS=Cucurbita maxima OX=3661 GN=LOC111497324 PE=4 SV=1)

HSP 1 Score: 2486.8 bits (6444), Expect = 0.0e+00
Identity = 1206/1363 (88.48%), Postives = 1273/1363 (93.40%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD+ FSD++LCGFL VVLAVPSPQSEL NALRPGTRCYVS E SDVCFTS+ GVVL+PIE
Sbjct: 2    DDDDFSDYKLCGFLCVVLAVPSPQSELVNALRPGTRCYVSVECSDVCFTSEYGVVLTPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
             NP+PLSK GVS QDSEQCRG VGGEG+ + E GD T KR++SARG R+S KKRTNRMG+
Sbjct: 62   ANPKPLSKAGVSPQDSEQCRGMVGGEGTSTVENGDLTLKRKMSARGRRTSGKKRTNRMGL 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            VHGSMSVVHQIHALV+HKCLKIDAQV FVDIG DE+AR VLLVDV+LP+ELWSGWQFPRS
Sbjct: 122  VHGSMSVVHQIHALVVHKCLKIDAQVIFVDIGVDEEARAVLLVDVHLPVELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSSILVGKD+S+ A M  KSVWN AECHVHNCKLHNS G S N
Sbjct: 182  KTVAGALFRHLSCEWQERSSILVGKDHSQVADMVRKSVWNPAECHVHNCKLHNSPGGSSN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            +RLFELHEIFRSLPSI +S KP+YTR+QPEDDYSQSGIWDISDD+LINI+KALRPLDLIR
Sbjct: 242  RRLFELHEIFRSLPSILRSCKPEYTRIQPEDDYSQSGIWDISDDVLINIMKALRPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTC HLRSL+ SIMPCMKLKLYPHQQAAVEWML RERN EV YHPLYVPFSTEDGFSF
Sbjct: 302  VASTCHHLRSLSASIMPCMKLKLYPHQQAAVEWMLRRERNAEVFYHPLYVPFSTEDGFSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVT G PAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPP GVQI+WC
Sbjct: 362  HINTVTGEIVTLGTPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPPGVQIVWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSSNSNTS+NH LVKEAVGC+SLKG+E+LT H PKR RL  LDDR+T+
Sbjct: 422  THNGNRKCGYYEVSSNSNTSTNHLLVKEAVGCSSLKGMEDLTYHTPKRARLTTLDDRHTV 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             +DSC  NE+ SPSS DYAKA+ MVRCTRSLSSV+RNLL T EGASSLS+ LN GKKSTR
Sbjct: 482  TDDSCASNEMISPSSTDYAKAVQMVRCTRSLSSVERNLLLTYEGASSLSRHLNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T T K AAGAKRAG+SNGFTN+ E+P TTT DKFE KDTWVQCDACHKWRKL+ETSIADA
Sbjct: 542  TRTGKLAAGAKRAGVSNGFTNNYEVPETTTADKFEXKDTWVQCDACHKWRKLSETSIADA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            S AWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKEN 
Sbjct: 602  STAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENM 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRALTWLS+L PEK+SEME  GLRSPIL SYVVPG DA  FH++FEAFGLVRK
Sbjct: 662  ALINSGTKRALTWLSNLAPEKISEMETTGLRSPILASYVVPGSDACAFHKMFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAAL+TAL+EPLDSVR YLSRATLI+VPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALKTALTEPLDSVRLYLSRATLIIVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQLLVYVWTDHRKPSAHCLAWD+DVIITTFSRLSAEWGP+K+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLLVYVWTDHRKPSAHCLAWDHDVIITTFSRLSAEWGPQKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMA+SL+SSNRWILTGTPTP+TPNSQLSHLQPLLRFLHEE YGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAVSLISSNRWILTGTPTPSTPNSQLSHLQPLLRFLHEEVYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLL+LLRRCMISARKTDLLSIPPCIKKVKYL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLDLLRRCMISARKTDLLSIPPCIKKVKYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            RSYNELVVTVRRNILMADWNDPSH+ESLLNPKQWK RSTTIKNIRLSCCVAGHIKV EAG
Sbjct: 962  RSYNELVVTVRRNILMADWNDPSHIESLLNPKQWKSRSTTIKNIRLSCCVAGHIKVAEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LD EGCTFPGCGKLYVMQTPET++RPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS
Sbjct: 1082 LDCEGCTFPGCGKLYVMQTPETISRPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKALSEANDEAAL PPPSL+KSD LLQEVDHSR I S HEIV EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALSEANDEAALFPPPSLTKSDVLLQEVDHSRNITSDHEIVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQLTIAGI FAGMYSPMH+SNKMKSL  FQHDASCMVLLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLTIAGIIFAGMYSPMHSSNKMKSLATFQHDASCMVLLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVTYVFLMEPIWD+SMEEQVISRAHRMGAT PIHVETLVM ETIEEQMVQFLQDTDECKR
Sbjct: 1262 FVTYVFLMEPIWDKSMEEQVISRAHRMGATHPIHVETLVMHETIEEQMVQFLQDTDECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+EEF   DYEGPRAHRS+ DFAGSNYLSQLKFVRTNPMMEK
Sbjct: 1322 LMKEEFNKPDYEGPRAHRSMQDFAGSNYLSQLKFVRTNPMMEK 1364

BLAST of Lag0040545 vs. ExPASy TrEMBL
Match: A0A6J1G0J1 (F-box protein At3g54460-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449582 PE=4 SV=1)

HSP 1 Score: 2471.0 bits (6403), Expect = 0.0e+00
Identity = 1206/1363 (88.48%), Postives = 1267/1363 (92.96%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD  FSD++LCGFL VVLAVPS  SELANALRPGTRCYVS E SDV FTS+NGVVLSPIE
Sbjct: 2    DDHDFSDYKLCGFLCVVLAVPSLPSELANALRPGTRCYVSDESSDVYFTSENGVVLSPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
            +NP+PLSKGGVSRQDSEQC GTVGG+G+GS E GDFTPK+EVS RGSRSSRKKRTNRMGM
Sbjct: 62   VNPKPLSKGGVSRQDSEQCGGTVGGDGTGSMETGDFTPKQEVSGRGSRSSRKKRTNRMGM 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            V+GSMS VHQIHALV+HKCLKIDAQV FVDIG  E+ARVVLLVDV+LPIELWSGWQFPRS
Sbjct: 122  VNGSMSAVHQIHALVVHKCLKIDAQVIFVDIGVYEEARVVLLVDVHLPIELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSSILVG+DY RDA M  KSVWNLAECHVHNCKLH SS  SPN
Sbjct: 182  KTVAGALFRHLSCEWQERSSILVGEDYLRDADMVRKSVWNLAECHVHNCKLHTSSEGSPN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            KRLFELHEIFRSLPSI KSSK D+TRM+PEDD++QSGIWDISDDILINILKAL PLDLIR
Sbjct: 242  KRLFELHEIFRSLPSIVKSSKSDHTRMKPEDDHTQSGIWDISDDILINILKALYPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTCRHL+SLA SIMPCMKLKLYPHQQAAVEWML+RER+ EV YHPLYVPFS EDG SF
Sbjct: 302  VASTCRHLKSLAASIMPCMKLKLYPHQQAAVEWMLHRERDVEVFYHPLYVPFSAEDGLSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVTGGAPAI+DFRGGLFCDEPGLGKTITALSLILKTQGT+AEPPAGV+IIWC
Sbjct: 362  HINTVTGEIVTGGAPAISDFRGGLFCDEPGLGKTITALSLILKTQGTIAEPPAGVKIIWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSS +N+SSNH LVKEA+  +SLKG E L  H PKR RL AL +R+T 
Sbjct: 422  THNGNRKCGYYEVSSKNNSSSNHCLVKEAMDFSSLKGFENLAFHTPKRARLTALVERHTA 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             ND C GN+LRSPSSADYAK +HMVRCTRSLS+VKRNLLFT EG SSLSKE N GKKSTR
Sbjct: 482  TNDLCAGNDLRSPSSADYAKDVHMVRCTRSLSTVKRNLLFTYEGVSSLSKEPNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRKFAAG KR  +SNGFT+  E+PG T  DK EYKDTWVQCDACHKWRKLAETS +DA
Sbjct: 542  TWTRKFAAGTKRDDVSNGFTSKFEVPGMTAADKLEYKDTWVQCDACHKWRKLAETSASDA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            SAAWFCSMNTDPFYQSCSVPEESYD+CRPITNIPGFY KETSGGEEKNISFFTSVLKEN 
Sbjct: 602  SAAWFCSMNTDPFYQSCSVPEESYDQCRPITNIPGFYRKETSGGEEKNISFFTSVLKENS 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRAL WLSSLT EKVSEMER GLRSPILTS VVPGGDARGFHQIFEAFGLVRK
Sbjct: 662  ALINSGTKRALNWLSSLTAEKVSEMERTGLRSPILTSSVVPGGDARGFHQIFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAALR+ALS PLDSVR YLSRATLIVVPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALRSALSAPLDSVRLYLSRATLIVVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQL+VYVWTDH+KPSAHCLAWDYDV+ITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLMVYVWTDHKKPSAHCLAWDYDVVITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            NHKSWEAGILRPFEAEMEEGR+LLLNLLRRCMISARK DLLSIPPCIKKV YL+FTEEHA
Sbjct: 902  NHKSWEAGILRPFEAEMEEGRLLLLNLLRRCMISARKADLLSIPPCIKKVTYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            R+YNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG
Sbjct: 962  RTYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LDSEGCTFPGCGKLYVMQT ETLARPENPNPKWPVP DLIELQPSYKQD+WDPDWQSTSS
Sbjct: 1082 LDSEGCTFPGCGKLYVMQTLETLARPENPNPKWPVPIDLIELQPSYKQDDWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRL AL EAND+ AL+PP S  K D LLQE+DHSR   S HE+V EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLTALGEANDKIALIPPSSFPKHDTLLQEIDHSRNFASDHEVVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQL IAGI+FAGMYSPMHASNKMKSLTMFQHD+SCM LLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLAIAGIKFAGMYSPMHASNKMKSLTMFQHDSSCMALLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVT+VFLMEPIWDRSMEEQVISRAHRMGA RPIHVETLVMRETIE+QMVQF QDTDE +R
Sbjct: 1262 FVTHVFLMEPIWDRSMEEQVISRAHRMGAVRPIHVETLVMRETIEQQMVQFRQDTDEFQR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+E+F  LDYEGPRAHRSLHDFAGSNYLSQLKFVRTNP ++K
Sbjct: 1322 LMKEDFDKLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPKIKK 1364

BLAST of Lag0040545 vs. ExPASy TrEMBL
Match: A0A6J1HPM8 (F-box protein At3g54460-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466564 PE=4 SV=1)

HSP 1 Score: 2468.7 bits (6397), Expect = 0.0e+00
Identity = 1205/1363 (88.41%), Postives = 1266/1363 (92.88%), Query Frame = 0

Query: 3    DDEHFSDFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLSPIE 62
            DD  FSD++LCGF  VVLAVPS QSELANALRPGTRCYVS E SDV FTS+NGVVLSPIE
Sbjct: 2    DDHDFSDYKLCGFFCVVLAVPSLQSELANALRPGTRCYVSDESSDVYFTSENGVVLSPIE 61

Query: 63   INPEPLSKGGVSRQDSEQCRGTVGGEGSGSTEVGDFTPKREVSARGSRSSRKKRTNRMGM 122
            +NP+PLSKGGVSRQDSEQC GTVGG+G+GS  +GDFTPKREVS RGSR+SRKKRTNRMGM
Sbjct: 62   VNPKPLSKGGVSRQDSEQCGGTVGGDGTGSMGMGDFTPKREVSGRGSRTSRKKRTNRMGM 121

Query: 123  VHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVYLPIELWSGWQFPRS 182
            V+GSMS VHQIHALV+HKCLKIDAQV FVDIG  E+ARVVLLVDV+LPIELWSGWQFPRS
Sbjct: 122  VNGSMSAVHQIHALVVHKCLKIDAQVIFVDIGVYEEARVVLLVDVHLPIELWSGWQFPRS 181

Query: 183  KTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHVHNCKLHNSSGDSPN 242
            KTVAGALFRHLSCEWQERSSILVG+DY RDA    KSVWNLAECHVHNCKLH SS  SPN
Sbjct: 182  KTVAGALFRHLSCEWQERSSILVGEDYLRDADTVRKSVWNLAECHVHNCKLHTSSEGSPN 241

Query: 243  KRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDILINILKALRPLDLIR 302
            KRLFELHEIFRSLPSI KSSK D+TRM+PEDD++QSGIWDISDDILINILKAL PLDLIR
Sbjct: 242  KRLFELHEIFRSLPSIVKSSKSDHTRMKPEDDHTQSGIWDISDDILINILKALCPLDLIR 301

Query: 303  VASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYHPLYVPFSTEDGFSF 362
            VASTCRHL+SLA SIMPCMKLKLYPHQQAAVEWML+RERN EV YHPLYVP S EDG SF
Sbjct: 302  VASTCRHLKSLAASIMPCMKLKLYPHQQAAVEWMLHRERNVEVFYHPLYVPVSAEDGLSF 361

Query: 363  HINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQGTLAEPPAGVQIIWC 422
            HINTVTGEIVTGGAPAI+DFRGGLFCDEPGLGKTITALSLILKTQGT+AEPPAGV+IIWC
Sbjct: 362  HINTVTGEIVTGGAPAISDFRGGLFCDEPGLGKTITALSLILKTQGTIAEPPAGVKIIWC 421

Query: 423  THNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKPKRPRLMALDDRYTI 482
            THNGNRKCGYYEVSS +N+SSNH LVKEA+  +SLKG E L  H PKR RL AL DR T 
Sbjct: 422  THNGNRKCGYYEVSSKNNSSSNHCLVKEAMDFSSLKGFENLAFHTPKRARLTALVDRRTA 481

Query: 483  DNDSCGGNELRSPSSADYAKAIHMVRCTRSLSSVKRNLLFTNEGASSLSKELNAGKKSTR 542
             ND C GN+LRSPSSADYAK +HMVRCTRSLS+VKRNLLFT EG SSLSKE N GKKSTR
Sbjct: 482  TNDLCAGNDLRSPSSADYAKDVHMVRCTRSLSTVKRNLLFTYEGMSSLSKEPNTGKKSTR 541

Query: 543  TLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFEYKDTWVQCDACHKWRKLAETSIADA 602
            T TRKFAAG KR  +SNGFT+  ++PG T  DK EYKDTWVQCDACHKWRKLAE S +DA
Sbjct: 542  TWTRKFAAGTKRDDVSNGFTSKFDVPGMTAADKLEYKDTWVQCDACHKWRKLAEISASDA 601

Query: 603  SAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPGFYSKETSGGEEKNISFFTSVLKENR 662
            SAAWFCSMNTDPFYQSCSVPEESYD+CRPITNIPGFYSKETSGGEEKNISFFTSVLKEN 
Sbjct: 602  SAAWFCSMNTDPFYQSCSVPEESYDQCRPITNIPGFYSKETSGGEEKNISFFTSVLKENS 661

Query: 663  ALINSGTKRALTWLSSLTPEKVSEMERNGLRSPILTSYVVPGGDARGFHQIFEAFGLVRK 722
            ALINSGTKRAL WLSSLT EKVSEMER GLRSPILTS VVPGGDARGFHQIFEAFGLVRK
Sbjct: 662  ALINSGTKRALNWLSSLTAEKVSEMERTGLRSPILTSSVVPGGDARGFHQIFEAFGLVRK 721

Query: 723  IEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQ 782
            +EKGT+RWYYPHNLHNLAFDVAALR+AL  PLDSVR YLSRATLIVVPSNLVDHWKTQIQ
Sbjct: 722  MEKGTIRWYYPHNLHNLAFDVAALRSALCAPLDSVRLYLSRATLIVVPSNLVDHWKTQIQ 781

Query: 783  KHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWGPRKKSILMQVHWHRVIL 842
            KHVRPGQL+VYVWTDH+KPSAHCLAWDYDV+ITTFSRLSAEWGPRK+SILMQVHWHRVIL
Sbjct: 782  KHVRPGQLMVYVWTDHKKPSAHCLAWDYDVVITTFSRLSAEWGPRKRSILMQVHWHRVIL 841

Query: 843  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 902
            DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ
Sbjct: 842  DEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQ 901

Query: 903  NHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTDLLSIPPCIKKVKYLHFTEEHA 962
            N+KSWEAGILRPFEAEMEEGR+LLLNLLRRCMISARK DLLSIPPCIKKV YL+FTEEHA
Sbjct: 902  NYKSWEAGILRPFEAEMEEGRLLLLNLLRRCMISARKADLLSIPPCIKKVTYLNFTEEHA 961

Query: 963  RSYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1022
            ++YNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG
Sbjct: 962  QTYNELVVTVRRNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAG 1021

Query: 1023 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1082
            EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA
Sbjct: 1022 EDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNCARCGEWCRLPVIAPCRHLLCLDCVA 1081

Query: 1083 LDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSS 1142
            LDSEGCTFPGCGKLYVMQT ETLARPENPNPKWPVP DLIELQPSYKQD+WDPDWQSTSS
Sbjct: 1082 LDSEGCTFPGCGKLYVMQTLETLARPENPNPKWPVPIDLIELQPSYKQDDWDPDWQSTSS 1141

Query: 1143 SKVAYLIQRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQ 1202
            SKVAYLIQRLKAL EAND+  L+PP S  K D LLQE+DHSR I S HE+V EKVLIFSQ
Sbjct: 1142 SKVAYLIQRLKALGEANDKITLIPPSSFHKHDTLLQEMDHSRTIASDHEVVREKVLIFSQ 1201

Query: 1203 FLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLS 1262
            FLEHIHVIEQQL IAGI+FAGMYSPMHASNKMKSLTMFQHD+SCM LLMDGSAALGLDLS
Sbjct: 1202 FLEHIHVIEQQLAIAGIKFAGMYSPMHASNKMKSLTMFQHDSSCMALLMDGSAALGLDLS 1261

Query: 1263 FVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKR 1322
            FVT+VFLMEPIWDRSMEEQVISRAHRMGA RPIHVETLVMRETIE+QMVQFLQDT ECKR
Sbjct: 1262 FVTHVFLMEPIWDRSMEEQVISRAHRMGAVRPIHVETLVMRETIEQQMVQFLQDTGECKR 1321

Query: 1323 LMREEFGNLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMMEK 1366
            LM+E+F  LDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPM++K
Sbjct: 1322 LMKEDFDKLDYEGPRAHRSLHDFAGSNYLSQLKFVRTNPMIKK 1364

BLAST of Lag0040545 vs. TAIR 10
Match: AT3G54460.1 (SNF2 domain-containing protein / helicase domain-containing protein / F-box family protein )

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 798/1398 (57.08%), Postives = 990/1398 (70.82%), Query Frame = 0

Query: 9    DFRLCGFLSVVLAVPSPQSELANALRPGTRCYVSGEGSDVCFTSQNGVVLS---PIEINP 68
            D +LCGFL  VL+V SP     + L+ G+ C++  +GS   F S+NG++LS   PI  N 
Sbjct: 4    DHKLCGFLCTVLSVDSP-----DLLQSGSSCFIFNDGSVTGFKSENGLILSLTNPIS-NL 63

Query: 69   EPL--SKGGVSRQDS-----------EQCRGTVGGEGSGSTEVGDFTPK---REVSARGS 128
            + L  SKG    ++S           ++ R  V GE SG  +    TPK   R +S    
Sbjct: 64   QSLISSKGDHDVENSGTIEDGRLETPQKRRKCVEGESSGKRK----TPKSKRRVLSGSKE 123

Query: 129  RSSR-KKRTNRMGMVHGSMSVVHQIHALVLHKCLKIDAQVTFVDIGGDEKARVVLLVDVY 188
            ++ + +KR   +GMV+GS+SVV Q+HALV +KCLKI  +V  VD G + + R V+LVDVY
Sbjct: 124  KTVQGRKRVKSIGMVNGSISVVQQLHALVANKCLKIICRVVKVDKGENGEERAVVLVDVY 183

Query: 189  LPIELWSGWQFPRSKTVAGALFRHLSCEWQERSSILVGKDYSRDAHMAGKSVWNLAECHV 248
            LPIELWSGWQFP+S+  A ALF+HLSC+W  R SIL GK    +A+   K++W+L++CHV
Sbjct: 184  LPIELWSGWQFPKSQATAAALFKHLSCDWGLRVSILDGKSIWEEANGRIKAIWDLSDCHV 243

Query: 249  HNCKLHNSSGDSPNKRLFELHEIFRSLPSIAKSSKPDYTRMQPEDDYSQSGIWDISDDIL 308
             +CKL  ++ +SP +RLF+LHEIF+SLPS         +R+ P  D   SG+WD+SDD+L
Sbjct: 244  FDCKLLCNAPNSPKRRLFKLHEIFKSLPSPGNHDVSYSSRVLPSTDSCVSGVWDLSDDVL 303

Query: 309  INILKALRPLDLIRVASTCRHLRSLAVSIMPCMKLKLYPHQQAAVEWMLYRERNPEVLYH 368
            I+IL  L   DL  +A+ CR  RSL   I+PCM LKL+PHQQAAV WML RER  EV  H
Sbjct: 304  ISILMKLDTKDLFSIAAVCRLFRSLTSLIVPCMNLKLFPHQQAAVGWMLERERKAEVSSH 363

Query: 369  PLYVPFSTEDGFSFHINTVTGEIVTGGAPAITDFRGGLFCDEPGLGKTITALSLILKTQG 428
            PLY+ F TEDGFSF++N VTG+I+T  AP + DFRGG+FCDEPGLGKTITALSLILKTQG
Sbjct: 364  PLYLSFDTEDGFSFYVNAVTGDIITEAAPMVKDFRGGMFCDEPGLGKTITALSLILKTQG 423

Query: 429  TLAEPPAGVQIIWCTHNGNRKCGYYEVSSNSNTSSNHFLVKEAVGCNSLKGLEELTCHKP 488
            T+A+PP G+ I+WCTH  ++KC YYE +S+  TS++   VK     +S +        +P
Sbjct: 424  TMADPPEGLPIVWCTHKSDKKCAYYEYTSDQFTSNSMSAVKRFQSPSSCRNQVSFEAFRP 483

Query: 489  ---------KRPRLMALDDRYTIDNDSCGGNELRS--PSSADYAKAIHMVRCTRSLSSVK 548
                     K+ RLM  DD+     +S   NE  +  P+S D        +C +SL +V+
Sbjct: 484  LLESKSLPFKQARLMDPDDQTLESKNSNFENEFETHIPASLDL-----KAQCRKSLGNVR 543

Query: 549  RNLLFTNEGASSLSKELNAGKKSTRTLTRKFAAGAKRAGMSNGFTNSCEMPGTTTEDKFE 608
            +NLL    GAS LS+ + A          K  +  K+ GM  G    C+  G T  D   
Sbjct: 544  KNLLPAYNGASELSEVMEA----------KRISNWKKCGMITG----CKRKGLTDSD--V 603

Query: 609  YKDTWVQCDACHKWRKLAETSIADASAAWFCSMNTDPFYQSCSVPEESYDKCRPITNIPG 668
              D W+QCD+C KWR++ +  ++   +AWFCS N DP YQSC+ PEE +DK +PI  + G
Sbjct: 604  ESDIWMQCDSCSKWRRIIDEGVSVTGSAWFCSNNNDPAYQSCNDPEELWDKSQPIKYLQG 663

Query: 669  FYSKETSGGEEKNISFFTSVLKENRALINSGTKRALTWLSSLTPEKVSEMERNGLRSPIL 728
            FY+K  SG E  NISFFTSVL+E+++ ++S  K+AL WL+ L  EK+S+ME  GL  P+L
Sbjct: 664  FYTKGASGEESDNISFFTSVLREHKSSVSSTVKKALIWLAKLPLEKLSQMETVGLPGPVL 723

Query: 729  TSYVVPGGDARGFHQIFEAFGLVRKIEKGTVRWYYPHNLHNLAFDVAALRTALSEPLDSV 788
               +    DA GF +IF AFGL  ++EKG  +W+YP  L NL FDV AL+ AL +PLD+ 
Sbjct: 724  GLKL----DALGFQRIFRAFGLKSRVEKGVTKWFYPKFLENLVFDVPALKVALCQPLDTF 783

Query: 789  RFYLSRATLIVVPSNLVDHWKTQIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTF 848
            R YLS+ATLIVVP+NLV+HW TQIQKHV   QL + VW DH + S H LAWDYDV+ITTF
Sbjct: 784  RLYLSKATLIVVPTNLVNHWLTQIQKHVCSDQLRILVWADHIELSPHSLAWDYDVVITTF 843

Query: 849  SRLSAEWGPRKKSILMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPT 908
            SRLSAEW PRKKS L+QVHW RV+LDEGHTLGSS++LTNK QMA+SL + NRW+LTGTPT
Sbjct: 844  SRLSAEWNPRKKSPLIQVHWLRVMLDEGHTLGSSVSLTNKFQMAVSLTACNRWLLTGTPT 903

Query: 909  PNTPNSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISA 968
            PNTPNSQLSH+QPLL+FLHEE YG+N K WEAGILRPFEAEMEEGR+ LL LL+RCMIS+
Sbjct: 904  PNTPNSQLSHIQPLLKFLHEEVYGENPKFWEAGILRPFEAEMEEGRLRLLQLLQRCMISS 963

Query: 969  RKTDLLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMADWNDPSHVESLLNPKQWK 1028
            RK DL  IPPCIKKV YL+F   HARSYNELV TVRRNIL+ADWNDPSHVESLLN KQWK
Sbjct: 964  RKKDLQMIPPCIKKVTYLNFLPGHARSYNELVETVRRNILLADWNDPSHVESLLNSKQWK 1023

Query: 1029 FRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDILVDDGLDPMSQEYSFIKYNLLYGGNC 1088
            FRS TI N+RLSCCVAGHIK+T+AG DI+ETMD L+++ LD  ++EYSFI+ +L+ G NC
Sbjct: 1024 FRSITISNVRLSCCVAGHIKMTDAGHDIKETMDALLENDLDLWTEEYSFIQDSLIGGCNC 1083

Query: 1089 ARCGEWCRLPVIAPCRHLLCLDCVALDSEGCTFPGCGKLYVMQTPETLARPENPNPKWPV 1148
             RCGEWCRLPVI PCRHLLCLDCVALDSE CT  GCG LY MQTPETLARPENPNPKWPV
Sbjct: 1084 KRCGEWCRLPVITPCRHLLCLDCVALDSERCTISGCGYLYEMQTPETLARPENPNPKWPV 1143

Query: 1149 PKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLKALSEANDEAALV-----------P 1208
            PKDLIELQPSYKQD+W+PDWQSTSSSKV+YL+ RL+ L E N ++ L             
Sbjct: 1144 PKDLIELQPSYKQDDWNPDWQSTSSSKVSYLVDRLRKLHEGNKKSILSFNKTDNDNLEDN 1203

Query: 1209 PPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHVIEQQLTIAGIRFAGMYS 1268
            PP    S+A L +  H +   S   + ++KVLIFSQFLEHIHVIEQQLT AGI+F  MYS
Sbjct: 1204 PP--GTSEAFLGKELHGQDCGS-QMVFVDKVLIFSQFLEHIHVIEQQLTTAGIKFGKMYS 1263

Query: 1269 PMHASNKMKSLTMFQHDASCMVLLMDGSAALGLDLSFVTYVFLMEPIWDRSMEEQVISRA 1328
            PM + NKMK+L MFQ+DA CM LLMDGS ALGLDLSFVT+VFLMEPIWD+S+EEQVISRA
Sbjct: 1264 PMQSYNKMKALAMFQNDADCMALLMDGSGALGLDLSFVTHVFLMEPIWDKSLEEQVISRA 1323

Query: 1329 HRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKRLMREEFGNLDYEGPRAHRSLHDFA 1365
            HRMGA RPI VETL MR TIEEQM++FL+D ++  RL+  ++     E  R+ R+LHD  
Sbjct: 1324 HRMGAKRPIFVETLTMRGTIEEQMMRFLEDAEKSDRLLSGDYIEAKQETTRSRRTLHDLV 1363

BLAST of Lag0040545 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 162.9 bits (411), Expect = 1.8e-39
Identity = 150/582 (25.77%), Postives = 241/582 (41.41%), Query Frame = 0

Query: 766  LIVVPSNLVDHWKTQIQKHVRPGQLLVYVWTDHRKPSAHCLAWDYDVIITTFSRLSAEWG 825
            LIV P  L+  WKT+I+ H +PG L VYV     +P    L    DV+ITT+  L++E+ 
Sbjct: 493  LIVCPMTLLGQWKTEIEMHAKPGSLSVYVHYGQSRPKDAKLLSQSDVVITTYGVLTSEFS 552

Query: 826  PRKKSI---LMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTPN 885
                +    +  V W R++LDE HT+ +S +  +    A +LV+  RW LTGTP  N   
Sbjct: 553  QENSADHEGIYAVRWFRIVLDEAHTIKNSKSQIS--LAAAALVADRRWCLTGTPIQN--- 612

Query: 886  SQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTD- 945
              L  L  LLRFL  E +G     W   + +PFE   E G  L+ ++L+  M+   K+  
Sbjct: 613  -NLEDLYSLLRFLRIEPWG-TWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTKSST 672

Query: 946  ------LLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMAD---------WNDPSH 1005
                  +L +PP   +V Y   +E     Y+ L    +R+ +  D          N  S 
Sbjct: 673  DREGRPILVLPPADARVIYCELSESERDFYDAL---FKRSKVKFDQFVEQGKVLHNYASI 732

Query: 1006 VESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDI--------------L 1065
            +E LL  +Q              CC   H  +  +  D  E  D+              L
Sbjct: 733  LELLLRLRQ--------------CC--DHPFLVMSRGDTAEYSDLNKLSKRFLSGKSSGL 792

Query: 1066 VDDGLDPMSQEY-SFIKYNLLYG--GNCARCGEWCRLPVIAPCRHLLCLDCVALDSEGCT 1125
              +G D  S+ +   +   L  G  G C  C E     V+ PC H LC +C+       T
Sbjct: 793  EREGKDVPSEAFVQEVVEELRKGEQGECPICLEALEDAVLTPCAHRLCRECLLASWRNST 852

Query: 1126 FPGCGKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLI 1185
               C                 P  +  V K  +   P+  +   D +     SSK+  L+
Sbjct: 853  SGLC-----------------PVCRNTVSKQELITAPTESRFQVDVEKNWVESSKITALL 912

Query: 1186 QRLKALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHV 1245
            + L+ L  +                                     K ++FSQ+   + +
Sbjct: 913  EELEGLRSSG-----------------------------------SKSILFSQWTAFLDL 972

Query: 1246 IEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSA-ALGLDLSFVTYVF 1305
            ++  L+     F  +   +    + K L  F  D S +VLLM   A  +G++L+  +  F
Sbjct: 973  LQIPLSRNNFSFVRLDGTLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAF 996

Query: 1306 LMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQM 1311
            +M+P W+ ++EEQ + R HR+G T+ + +   +++ T+EE+M
Sbjct: 1033 VMDPWWNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERM 996

BLAST of Lag0040545 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 149.4 bits (376), Expect = 2.1e-35
Identity = 159/580 (27.41%), Postives = 241/580 (41.55%), Query Frame = 0

Query: 765  TLIVVPSNLVDHWKTQIQKHVRPG--QLLVYVWTDHRKPSAHCLAWDYDVIITTFSRL-S 824
            TLI+ P  L+  WK +++ H +P    +LVY   D R   A  +A  +DV++TT+  L S
Sbjct: 743  TLIICPMALLSQWKDELETHSKPDTVSVLVYYGGD-RTHDAKAIA-SHDVVLTTYGVLTS 802

Query: 825  AEWGPRKKSILMQVHWHRVILDEGHTLGSSLNLTNKLQMAISLVSSNRWILTGTPTPNTP 884
            A       SI  ++ W+R++LDE HT+ S    T   +    L S  RW LTGTP  N  
Sbjct: 803  AYKQDMANSIFHRIDWYRIVLDEAHTIKSW--KTQAAKATFELSSHCRWCLTGTPLQN-- 862

Query: 885  NSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMISARKTD 944
              +L  L  LL FLH E +  N   W   I +P+E     G  L+  +LR  M+   K  
Sbjct: 863  --KLEDLYSLLCFLHVEPW-CNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKET 922

Query: 945  -------LLSIPPCIKKVKYLHFTEEHARSYNELVVTVRRNILMAD---------WNDPS 1004
                   +L +PP   +V     +E     Y  L    +R+ +  D          N  +
Sbjct: 923  RDKEGSLILELPPTDVQVIECEQSEAERDFYTAL---FKRSKVQFDQFVAQGKVLHNYAN 982

Query: 1005 HVESLLNPKQWKFRSTTIKNIRLSCCVAGHIKVTEAGEDIQETMDIL----VDDGLDPMS 1064
             +E LL  +Q              CC    + ++ A       +D L    +D+  D +S
Sbjct: 983  ILELLLRLRQ--------------CCNHPFLVMSRADSQQYADLDSLARRFLDNNPDSVS 1042

Query: 1065 QEYSFIKY-----NLLYGGN---CARCGEWCRLPVIAPCRHLLCLDCVALDSEGCTFPGC 1124
            Q      Y       L  GN   C  C E    PV+ PC H +C +C+         P C
Sbjct: 1043 QNAPSRAYIEEVIQDLRDGNSKECPICLESADDPVLTPCAHRMCRECLLTSWRS---PSC 1102

Query: 1125 GKLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLK 1184
            G   + +T   L R E  +     P D I      K  NW        SSKV+ L++ L+
Sbjct: 1103 GLCPICRT--ILKRTELIS----CPTDSIFRVDVVK--NW------KESSKVSELLKCLE 1162

Query: 1185 ALSEANDEAALVPPPSLSKSDALLQEVDHSRAIPSYHEIVIEKVLIFSQFLEHIHVIEQQ 1244
             + ++                                    EK ++FSQ+   + ++E  
Sbjct: 1163 KIKKSGSG---------------------------------EKSIVFSQWTSFLDLLEIP 1222

Query: 1245 LTIAGIRFAGMYSPMHASNKMKSLTMFQHDASCMVLLMDGSA-ALGLDLSFVTYVFLMEP 1304
            L   G  F      +    + K L  F       +LLM   A  +GL+L+  + VFLM+P
Sbjct: 1223 LRRRGFEFLRFDGKLAQKGREKVLKEFNETKQKTILLMSLKAGGVGLNLTAASSVFLMDP 1246

Query: 1305 IWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQ 1313
             W+ ++EEQ I R HR+G  R + V   ++++T+EE+M Q
Sbjct: 1283 WWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQ 1246

BLAST of Lag0040545 vs. TAIR 10
Match: AT1G11100.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 127.9 bits (320), Expect = 6.5e-29
Identity = 150/643 (23.33%), Postives = 286/643 (44.48%), Query Frame = 0

Query: 750  LSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQKHVR-PGQLLVYVWTD-HRKPSAHCLA 809
            + + L  +R   +  TLIV P++L+  W  +++K V     L V V+    R    H LA
Sbjct: 620  VEDSLCKMRGRPAAGTLIVCPTSLMRQWADELRKKVTLEAHLSVLVYHGCSRTKDPHELA 679

Query: 810  WDYDVIITTFSRLSAEWGPRKKSI------LMQVHWHRVILDEGHTLGSSLNLTNKLQMA 869
              YDV+ITT+S +S       + +      L QV W RV+LDE  ++    N   +  +A
Sbjct: 680  -KYDVVITTYSLVSKRKHMDCEPVEFLSGPLAQVSWFRVVLDEAQSI---KNYKTQASIA 739

Query: 870  IS-LVSSNRWILTGTPTPNTPNSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEME 929
             S L +  RW L+GTP  N+    ++ L    RFL  + Y  +++++   I  P  +   
Sbjct: 740  CSGLHAKRRWCLSGTPIQNS----IADLYSYFRFLKYDPY-SSYQTFCETIKNPISSYPG 799

Query: 930  EGRILLLNLLRRCMISARKTDLL------SIPPCIKKVKYLHFTEEHARSYNELVVTVR- 989
            EG   L  +L++ M+   K  LL      S+PP   +++ + FT+E    Y++L    R 
Sbjct: 800  EGYKTLQAILKKVMLRRTKDTLLDGKPVISLPPKSIELRRVDFTKEERDFYSKLECDSRD 859

Query: 990  --RNILMADWNDPSHVESLLNPKQWKFRSTTIKNIRLSC---CVAGHIKVTEAGEDIQET 1049
              +    A     ++V  LL           +  +R +C    +   +  + + E +++ 
Sbjct: 860  QFKEYAEAGTVKQNYVNILL----------MLLRLRQACGHPLLVSSLSWSSSAEMVKKL 919

Query: 1050 MDILVDDGLDPMSQEYSFIKYNLLYG-GNCARCGEWCRLPVIAPCRHLLC----LDCVAL 1109
                         ++ +F+ + L      C  C    +  V++ C H+ C     +C+  
Sbjct: 920  -----------PYEKLTFLLHRLEASLAICGICNVAPKDAVVSLCGHVFCNQCICECLTR 979

Query: 1110 DSEGCTFPGCG---KLYVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQST 1169
            D+  C    C    ++  + + ETL        K   P D     P    +  +      
Sbjct: 980  DNNQCPLSYCKVGLEISSLFSRETLENAMLDLHKLDAPCDRTTSDPVGSGEPCEN--LPC 1039

Query: 1170 SSSKVAYLIQRLKALSEANDEAALVPPPSLSKSDA-LLQEVDHSRAIPS---------YH 1229
             SSK+   +  L++LS     A ++   + S  +    Q++D S ++P+           
Sbjct: 1040 GSSKIKAALDILQSLSRPQSPATVMNDVNQSSENGENNQQLDKSFSLPATPAKSSVGGVV 1099

Query: 1230 EIVIEKVLIFSQFLEHIHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQ--HDASCMV 1289
             +  EK ++F+Q+ + + ++E  L  +GI++      M    +  ++  F    D S M+
Sbjct: 1100 NVAGEKAIVFTQWTKMLDLLEAGLKSSGIQYRRFDGKMTVPARDAAVQDFNTLPDVSVMI 1159

Query: 1290 LLMDGSAALGLDLSFVTYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEE 1349
            + +  +A+LGL++    +V +++  W+ + E+Q I RAHR+G TRP+ V    +++T+E+
Sbjct: 1160 MSLK-AASLGLNMVAACHVIMLDLWWNPTTEDQAIDRAHRIGQTRPVKVVRFTVKDTVED 1219

Query: 1350 QMVQFLQDTDECKRLMREEFGNLDYEGPRAHRSLHDFAGSNYL 1352
            +++   Q   + ++++   FG  +     +H S+ D    NYL
Sbjct: 1220 RILALQQ---KKRKMVASAFGEHENGSRESHLSVEDL---NYL 1223

BLAST of Lag0040545 vs. TAIR 10
Match: AT1G11100.2 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 119.4 bits (298), Expect = 2.3e-26
Identity = 154/687 (22.42%), Postives = 290/687 (42.21%), Query Frame = 0

Query: 750  LSEPLDSVRFYLSRATLIVVPSNLVDHWKTQIQKHVR-PGQLLVYVWTD-HRKPSAHCLA 809
            + + L  +R   +  TLIV P++L+  W  +++K V     L V V+    R    H LA
Sbjct: 619  VEDSLCKMRGRPAAGTLIVCPTSLMRQWADELRKKVTLEAHLSVLVYHGCSRTKDPHELA 678

Query: 810  WDYDVIITTFSRLSAE-------------------------WGPRKKSI----------- 869
              YDV+ITT+S +S E                         +G  KK +           
Sbjct: 679  -KYDVVITTYSLVSVEVPKQPRDRADEEKGGIHDGGVESVGFGSNKKDLPNSQKKGTKKR 738

Query: 870  --------------LMQVHWHRVILDEGHTLGSSLNLTNKLQMAIS-LVSSNRWILTGTP 929
                          L QV W RV+LDE  ++    N   +  +A S L +  RW L+GTP
Sbjct: 739  KHMDCEPVEFLSGPLAQVSWFRVVLDEAQSI---KNYKTQASIACSGLHAKRRWCLSGTP 798

Query: 930  TPNTPNSQLSHLQPLLRFLHEEAYGQNHKSWEAGILRPFEAEMEEGRILLLNLLRRCMIS 989
              N+    ++ L    RFL  + Y  +++++   I  P  +   EG   L  +L++ M+ 
Sbjct: 799  IQNS----IADLYSYFRFLKYDPY-SSYQTFCETIKNPISSYPGEGYKTLQAILKKVMLR 858

Query: 990  ARKTDLL------SIPPCIKKVKYLHFTEEHARSYNELVVTVR---RNILMADWNDPSHV 1049
              K  LL      S+PP   +++ + FT+E    Y++L    R   +    A     ++V
Sbjct: 859  RTKDTLLDGKPVISLPPKSIELRRVDFTKEERDFYSKLECDSRDQFKEYAEAGTVKQNYV 918

Query: 1050 ESLLNPKQWKFRSTTIKNIRLSC---CVAGHIKVTEAGEDIQETMDILVDDGLDPMSQEY 1109
              LL           +  +R +C    +   +  + + E +++              ++ 
Sbjct: 919  NILL----------MLLRLRQACGHPLLVSSLSWSSSAEMVKKL-----------PYEKL 978

Query: 1110 SFIKYNLLYG-GNCARCGEWCRLPVIAPCRHLLC----LDCVALDSEGCTFPGCG---KL 1169
            +F+ + L      C  C    +  V++ C H+ C     +C+  D+  C    C    ++
Sbjct: 979  TFLLHRLEASLAICGICNVAPKDAVVSLCGHVFCNQCICECLTRDNNQCPLSYCKVGLEI 1038

Query: 1170 YVMQTPETLARPENPNPKWPVPKDLIELQPSYKQDNWDPDWQSTSSSKVAYLIQRLKALS 1229
              + + ETL        K   P D     P    +  +       SSK+   +  L++LS
Sbjct: 1039 SSLFSRETLENAMLDLHKLDAPCDRTTSDPVGSGEPCEN--LPCGSSKIKAALDILQSLS 1098

Query: 1230 EANDEAALVPPPSLSKSDA-LLQEVDHSRAIPS---------YHEIVIEKVLIFSQFLEH 1289
                 A ++   + S  +    Q++D S ++P+            +  EK ++F+Q+ + 
Sbjct: 1099 RPQSPATVMNDVNQSSENGENNQQLDKSFSLPATPAKSSVGGVVNVAGEKAIVFTQWTKM 1158

Query: 1290 IHVIEQQLTIAGIRFAGMYSPMHASNKMKSLTMFQ--HDASCMVLLMDGSAALGLDLSFV 1349
            + ++E  L  +GI++      M    +  ++  F    D S M++ +  +A+LGL++   
Sbjct: 1159 LDLLEAGLKSSGIQYRRFDGKMTVPARDAAVQDFNTLPDVSVMIMSLK-AASLGLNMVAA 1218

Query: 1350 TYVFLMEPIWDRSMEEQVISRAHRMGATRPIHVETLVMRETIEEQMVQFLQDTDECKRLM 1352
             +V +++  W+ + E+Q I RAHR+G TRP+ V    +++T+E++++   Q   + ++++
Sbjct: 1219 CHVIMLDLWWNPTTEDQAIDRAHRIGQTRPVKVVRFTVKDTVEDRILALQQ---KKRKMV 1266