Homology
BLAST of Lag0040461 vs. NCBI nr
Match:
XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])
HSP 1 Score: 982.2 bits (2538), Expect = 1.8e-282
Identity = 504/564 (89.36%), Postives = 531/564 (94.15%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS EESCTAP AVE+R NS GGY+VNTAVYHS VQ+DEA KIST PDLDTATFVLSQFP
Sbjct: 1 MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTT+P YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIESFE +P+T+++QSD AAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQFKQQQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQVLSKL 564
BLAST of Lag0040461 vs. NCBI nr
Match:
KAG7031989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 979.9 bits (2532), Expect = 9.0e-282
Identity = 505/564 (89.54%), Postives = 532/564 (94.33%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS EESCTAP AVE+R NS GGY+VNTAVYHSLVQ+DEASKIST PDLDTATFVLSQFP
Sbjct: 1 MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIE+FE +PKT+++QSDTAAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGW+KTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563
BLAST of Lag0040461 vs. NCBI nr
Match:
XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])
HSP 1 Score: 977.2 bits (2525), Expect = 5.8e-281
Identity = 504/564 (89.36%), Postives = 530/564 (93.97%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS EESCTAP AVE+R NS GGY+VNTAVYHSLVQ DEA KIST PDLDTATFVLSQFP
Sbjct: 1 MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTG+PTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIESFE +PKT+++QSDTAAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGW+KTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563
BLAST of Lag0040461 vs. NCBI nr
Match:
XP_038891768.1 (4-coumarate--CoA ligase-like 5 [Benincasa hispida])
HSP 1 Score: 974.2 bits (2517), Expect = 4.9e-280
Identity = 502/564 (89.01%), Postives = 529/564 (93.79%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MSGEESCTAP AVE++ +NS GGYNV TAVYHSL+Q+DEA ISTR DL+TATFVLSQFP
Sbjct: 1 MSGEESCTAPAAVEFQPQNSPGGYNVKTAVYHSLIQLDEAPIISTRQDLNTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYGQLS SIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AV+SIGAVITTANP+NTASEIGKQVRDSGAKLAVSAPEELHK+SPTGIPTILTTR YGD
Sbjct: 121 AVMSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELHKLSPTGIPTILTTRSSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
LSVEELIESC ESFEPLPK E++QSD AAILYSSGTTGTSKGVVLTHSN+I V+ELL W
Sbjct: 181 TLSVEELIESCSESFEPLPKIEVTQSDPAAILYSSGTTGTSKGVVLTHSNIISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
VD++SSQ+DVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMI AIEK+K+NN
Sbjct: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIAAIEKHKINN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKSG GSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSGDGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDAKAHPGSCGMLM F AKIVDVETGEG+ PMKEGELWLKSPTIMK YLG
Sbjct: 361 GAATWMITDKDAKAHPGSCGMLMPSFFAKIVDVETGEGVAPMKEGELWLKSPTIMKGYLG 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+D+EGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATIDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIPMEDEAAGQIPVACVVKA +LTEEQVIQFVASQVA YKKVRGVRF+ +IPR
Sbjct: 481 ILDAAVIPMEDEAAGQIPVACVVKAPTSKLTEEQVIQFVASQVAAYKKVRGVRFISSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQFKQQQLLSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQLLSKL 564
BLAST of Lag0040461 vs. NCBI nr
Match:
KAG6601192.1 (4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 974.2 bits (2517), Expect = 4.9e-280
Identity = 503/564 (89.18%), Postives = 530/564 (93.97%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS ESCTAP AVE+R NS G Y+VNTAVYHSLVQ+DEASKIST PDLDTATFVLSQFP
Sbjct: 1 MSRAESCTAPAAVEFRPMNSPGVYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIE+FE +PKT+++QSDTAAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGW+KTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563
BLAST of Lag0040461 vs. ExPASy Swiss-Prot
Match:
M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)
HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-126
Identity = 245/539 (45.45%), Postives = 350/539 (64.94%), Query Frame = 0
Query: 23 GYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQL 82
GY + ++++S E ++ +D TF+ S+ ++A ID+ T +T+ QL
Sbjct: 14 GYCKSNSIFYSK---REPVQLPQNHSVDVTTFISSR--AHHGKIAFIDAATGRHLTFPQL 73
Query: 83 SASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 142
++ S+A L A+G+RKGDV+ +LS NS+ +PV+CLAV+S+GA+ITT NP+NT EI
Sbjct: 74 WRAVDSVATCL-SAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIA 133
Query: 143 KQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDILSVEELIESCIESF--EPLPK 202
KQ+ DS LA + P+ + K++ + +P ++ + ++ S E EP P
Sbjct: 134 KQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPN 193
Query: 203 T---EMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIP 262
++Q DTA +LYSSGTTG SKGVV +H NLI +++ ++ ++ F+C +P
Sbjct: 194 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEH-TFICTVP 253
Query: 263 MFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG-- 322
MFHIYGL F +GL G T V++ +F M+ AIEKY+ +P VPP+++ L+K+
Sbjct: 254 MFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANH 313
Query: 323 --GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDKDAK 382
DLSSL+ + SG APL K+V E F E +P V + GYGLTESTG T ++++
Sbjct: 314 IRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESR 373
Query: 383 AHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEG 442
+ G+ GML AKIV+ ETGE L + GELWL+ PTIMK Y N+EAT +T+D EG
Sbjct: 374 RY-GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEG 433
Query: 443 WLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEA 502
WL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LLSHPEI DAAVIP D+
Sbjct: 434 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKE 493
Query: 503 AGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
AGQ P+A VV+ L+E V+ F+A VAPYK++R V F+ +IP++ +GKILRKDL+
Sbjct: 494 AGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLI 544
BLAST of Lag0040461 vs. ExPASy Swiss-Prot
Match:
Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)
HSP 1 Score: 448.7 bits (1153), Expect = 9.4e-125
Identity = 244/542 (45.02%), Postives = 347/542 (64.02%), Query Frame = 0
Query: 19 NSSGGY-NVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRV 78
NS G+ N N+ Y I + P LD TF+ SQ R+A ID+ T +
Sbjct: 5 NSRSGFCNSNSTFYSKRTPI----PLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64
Query: 79 TYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNT 138
T+ +L ++ S+A L +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65 TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124
Query: 139 ASEIGKQVRDSGAKLAVSAPEELHKVSPTG--IPTILTTRPPYGDILSVEELIESC-IES 198
++EI KQ++DS LA + + L K+S +P +L + V L+E E
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184
Query: 199 FEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLC 258
K + Q DTA +LYSSGTTG SKGV+ +H NLI +++ ++ + + F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244
Query: 259 FIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKS 318
+PMFHIYGL F GL G T +++ +F M+ AI KY+ ++P VPP+++ +V
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304
Query: 319 G----GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDK 378
DLSS+ + G APL K+V E F EK+P V++ GYGLTESTG T +
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364
Query: 379 DAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLD 438
+++ + G+ G L + +IVD TG+ L P + GELWLK P+IMK Y N+EAT +TLD
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424
Query: 439 KEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPME 498
EGWL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LL+HPEI DAAVIP
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484
Query: 499 DEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKD 553
D+ GQ P+A VV+ + L+E+ +++FVA QVAPYK++R V F+ +IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537
BLAST of Lag0040461 vs. ExPASy Swiss-Prot
Match:
Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)
HSP 1 Score: 432.6 bits (1111), Expect = 7.0e-120
Identity = 235/522 (45.02%), Postives = 332/522 (63.60%), Query Frame = 0
Query: 47 PDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVF 106
P+ +F+ + S++A+ DS T +T+ QL +++ LA G +H LG+RK DVV
Sbjct: 25 PNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG-FHRLGIRKNDVVL 84
Query: 107 VLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPT 166
+ + NS +P+ LAV +IG V TTANP+ T +E+ KQ++DS K+ +S + K+
Sbjct: 85 IFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGF 144
Query: 167 GIPTIL-----TTRPPYGD---ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTT 226
+P +L T P G ILS + ++E E P E+ QSDTAA+LYSSGTT
Sbjct: 145 DLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFVEIKQSDTAALLYSSGTT 204
Query: 227 GTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITT 286
GTSKGV LTH N I ++ D + VFLCF+PMFH++GL RG
Sbjct: 205 GTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNAL 264
Query: 287 VLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKD 346
V M RF + ++ IEK++V ++ VPPV L L K DLSSL+ IGSGAAPLGKD
Sbjct: 265 VSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKD 324
Query: 347 VEEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETG 406
+ E P V L GYG+TE+ G ++ + K + GS GML A+IV VETG
Sbjct: 325 LMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETG 384
Query: 407 EGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIK 466
+ PP ++GE+W++ P +MK YL N +AT+ T+DK+ W+ TGDL Y +EDG +Y+VDRIK
Sbjct: 385 KSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIK 444
Query: 467 ELIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVI 526
ELIK+ G+QVAPAELE +L+SHP+I+DA VIP DE AG++P+A VV++ N +TE+ +
Sbjct: 445 ELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQ 504
Query: 527 QFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFKQQ 559
+F+A QVAPYK++R V F+ +P+S AGKILR++LV Q + +
Sbjct: 505 KFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543
BLAST of Lag0040461 vs. ExPASy Swiss-Prot
Match:
P0C5B6 (OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1)
HSP 1 Score: 429.1 bits (1102), Expect = 7.7e-119
Identity = 230/521 (44.15%), Postives = 332/521 (63.72%), Query Frame = 0
Query: 43 ISTRPDL--DTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVR 102
+S P+L D TF+ SQ + A ID+ T +T+ L ++ +A LYH +G+R
Sbjct: 30 LSLPPNLSRDVTTFISSQ--PHRGKTAFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIR 89
Query: 103 KGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEEL 162
+GDVV +LS NS+ PV+CL+V+S+GAV TTAN +NT+ EI KQ+ DS L V +L
Sbjct: 90 RGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIADSNPTL-VFTTRQL 149
Query: 163 HKVSPTGIPTILTTRPPYGDILSVEELIESCIESFEPLP-----KTEMSQSDTAAILYSS 222
P I +LT Y ++ S ++ E + P + ++Q DTA +LYSS
Sbjct: 150 APKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSS 209
Query: 223 GTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRG 282
GTTG SKGV+ +H NL + ++ + ++D+F+C +PMFH YGL+ F +G G
Sbjct: 210 GTTGPSKGVISSHRNLTAHVAR---FISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALG 269
Query: 283 ITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAA 342
T V+++RF M+DA+EK++ + PPV++ ++ DLSSL+ + G A
Sbjct: 270 STVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGA 329
Query: 343 PLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIV 402
PL K+V E F EK+P V++ GY LTES G T ++++ + G+ G L S A+IV
Sbjct: 330 PLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRY-GTAGTLTSDVEARIV 389
Query: 403 DVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYI 462
D TG + + GELWLK P+I K Y N+EAT T++ EGWLKTGDL YIDEDGF+++
Sbjct: 390 DPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFV 449
Query: 463 VDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLT 522
VDR+KELIK+ GYQV PAELE +L++HP+I+DAAVIP D+ AGQ P+A VV+ L+
Sbjct: 450 VDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLS 509
Query: 523 EEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
E+QVI F++ QVAPYKK+R V F+++IP++ +GK LRKDL+
Sbjct: 510 EKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 543
BLAST of Lag0040461 vs. ExPASy Swiss-Prot
Match:
M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)
HSP 1 Score: 421.0 bits (1081), Expect = 2.1e-116
Identity = 228/519 (43.93%), Postives = 331/519 (63.78%), Query Frame = 0
Query: 48 DLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFV 107
+L +FV + + ALIDS T+ +++ Q + + ++ G + LGV+K DVV +
Sbjct: 26 NLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQFKSMVIKVSHGFLN-LGVQKNDVVLI 85
Query: 108 LSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTG 167
+ NS+ PV L +++ GA+ TT+NP+ T SE+ KQV+DS KL V+ PE KV
Sbjct: 86 FAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSKQVKDSNPKLIVTVPELFEKVKGFN 145
Query: 168 IPTIL--------TTRPPYGDILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTG 227
+PTIL + +L+ +L+ + P + QSDTAA+LYSSGTTG
Sbjct: 146 LPTILIGPNSEDSSPLKSRAKVLTFHDLV-TLSGPVSDFPMVDFKQSDTAALLYSSGTTG 205
Query: 228 TSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTV 287
SKGVVL+H N I ++ D + ++VFLCF+PMFH++GL RG T +
Sbjct: 206 MSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFLPMFHVFGLAIITYAQLQRGNTVI 265
Query: 288 LMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKDV 347
M RF+ + ++ +EKYKV ++ VPPVIL L K+ DLSSL+ IGSGAAPLGKD+
Sbjct: 266 SMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSIVKKYDLSSLKHIGSGAAPLGKDL 325
Query: 348 EEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGE 407
E + P + GYG+TE+ G ++ + K H GS GML S A+IV V+T +
Sbjct: 326 MEECAKIVPHGIVAQGYGMTETCGIVSVE-DTRGGKRHTGSAGMLSSGVEAQIVSVDTLK 385
Query: 408 GLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKE 467
LPP + GE+W++ P +M+ Y N AT+ T+DK+GW+ TGDL Y DEDG +Y+VDRIKE
Sbjct: 386 PLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRIKE 445
Query: 468 LIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQ 527
LIK+ G+QVAPAELE +L+SHPEI+DA VIP D AG++PVA VV++ N LTE+ V +
Sbjct: 446 LIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAGEVPVAYVVRSPNSSLTEDDVKK 505
Query: 528 FVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFK 557
F+A QVA +K++R V F++++P+S +GKILR++L+ + +
Sbjct: 506 FIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKVR 541
BLAST of Lag0040461 vs. ExPASy TrEMBL
Match:
A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)
HSP 1 Score: 982.2 bits (2538), Expect = 8.8e-283
Identity = 504/564 (89.36%), Postives = 531/564 (94.15%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS EESCTAP AVE+R NS GGY+VNTAVYHS VQ+DEA KIST PDLDTATFVLSQFP
Sbjct: 1 MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTT+P YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIESFE +P+T+++QSD AAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQFKQQQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQVLSKL 564
BLAST of Lag0040461 vs. ExPASy TrEMBL
Match:
A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)
HSP 1 Score: 977.2 bits (2525), Expect = 2.8e-281
Identity = 504/564 (89.36%), Postives = 530/564 (93.97%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFP 60
MS EESCTAP AVE+R NS GGY+VNTAVYHSLVQ DEA KIST PDLDTATFVLSQFP
Sbjct: 1 MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60
Query: 61 TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61 TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120
Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTG+PTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180
Query: 181 ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIW 240
+LSVEELIESCIESFE +PKT+++QSDTAAILYSSGTTGTSKGVVLTHSNLI V+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240
Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300
Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
Query: 361 GAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLG 420
GAAT I DKDA+AHPGSCGMLM RF AKIVD+ETGEGLPPMKEGE+WLKSPTIMKEYL
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420
Query: 421 NKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
NKEATEAT+DKEGW+KTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480
Query: 481 IIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
I+DAAVIP EDEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540
Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563
BLAST of Lag0040461 vs. ExPASy TrEMBL
Match:
A0A6J1CEK1 (4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE=4 SV=1)
HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 500/571 (87.57%), Postives = 531/571 (92.99%), Query Frame = 0
Query: 1 MSGEESCTAPDAVEYRRRN------SSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATF 60
MSGEES TAP A EY R+N S GGY+VN AVYHSLV+ DEA KIS+RPDLDTATF
Sbjct: 1 MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEARKISSRPDLDTATF 60
Query: 61 VLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVL 120
VLSQFP AESRVALIDSVTSSRVTYGQLSASIRSLACGL+HALGVRKGDVVFVLSANS+L
Sbjct: 61 VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
Query: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTT 180
YPVICLAVLS+GAVITTANPVNTASEI KQVRDSGAKLAVSAPEE+HK++PTGIPTILTT
Sbjct: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
Query: 181 RPPYG-DILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLIC 240
RP G D+LSVEELIESC +S E LP TE++QSDTAAILYSSGTTGTSKGVVLTHSNLI
Sbjct: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
Query: 241 VMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAI 300
V+ELL YVDASSS+NDVFLCFIPMFHIYGLVFFGLGLFCRGIT +LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360
EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
Query: 361 YGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPT 420
YGLTESTGAAT I D DAK HPGSCGMLM RFSAKIVD+ETGEGLPPMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
Query: 421 IMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELET 480
IMK YLGN+EATEAT+D+EGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
Query: 481 ILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVR 540
IL+ H EI+DAAVIPMEDEAAGQIPVACVVKAS QLTE+QVIQFVASQVAPYKKVRGV+
Sbjct: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
Query: 541 FMDAIPRSLAGKILRKDLVSQFKQQQLLSKL 565
F++AIPRSLAGKILRKDLVSQFKQQQL SKL
Sbjct: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
BLAST of Lag0040461 vs. ExPASy TrEMBL
Match:
A0A1S3BFZ3 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)
HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 500/566 (88.34%), Postives = 525/566 (92.76%), Query Frame = 0
Query: 1 MSGEESC-TAPDAVEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQF 60
MS EESC T P A+E++ RNS GGYNVNTAVYHSL+ DEA ISTR DLDTAT+VLSQF
Sbjct: 1 MSDEESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQF 60
Query: 61 PTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVIC 120
PTAESRVALIDSVTS RVTYGQL SIRSLACGLYHALGVRKGDVVFVLSAN VLYPVIC
Sbjct: 61 PTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVIC 120
Query: 121 LAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYG 180
LAVLSIGAVITTANP+NTASEIGKQVRDSGAKLAVSAPEEL K+ PTGIPTILTTR +
Sbjct: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFS 180
Query: 181 DILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLI 240
D LSVEELIESC ES EPLPKTE++QSDTAAILYSSGTTGTSKGVVLTHSNLI V+ELL
Sbjct: 181 DALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240
Query: 241 WYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVN 300
W VD++SSQ+DVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYK+N
Sbjct: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKIN 300
Query: 301 NIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360
NIPAVPPVILGLVKSGGGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTES
Sbjct: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360
Query: 361 TGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYL 420
TGAAT I DKDAKAHPGSCGMLM F KIVDVETGEGLPPMK+GELWLKSPTIMKEYL
Sbjct: 361 TGAATWMITDKDAKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYL 420
Query: 421 GNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHP 480
GN+EATEATLD+EGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH
Sbjct: 421 GNREATEATLDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHT 480
Query: 481 EIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIP 540
EI+DAAVIP+EDEAAGQIPVACVVKA +C LTE+QVIQFVASQVAPYKKVRGVRF+ AIP
Sbjct: 481 EILDAAVIPIEDEAAGQIPVACVVKAPSCVLTEQQVIQFVASQVAPYKKVRGVRFISAIP 540
Query: 541 RSLAGKILRKDLVSQFK-QQQLLSKL 565
RSLAGKILRKDLVSQFK QQQL SKL
Sbjct: 541 RSLAGKILRKDLVSQFKQQQQLFSKL 566
BLAST of Lag0040461 vs. ExPASy TrEMBL
Match:
A0A0A0KRI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1)
HSP 1 Score: 937.9 bits (2423), Expect = 1.9e-269
Identity = 485/553 (87.70%), Postives = 515/553 (93.13%), Query Frame = 0
Query: 13 VEYRRRNSSGGYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSV 72
VE++ ++S GGYNVNTAVYHSL+ +DEA ISTR DLDTAT+VLSQFPTAESRVALIDSV
Sbjct: 7 VEFQPQSSPGGYNVNTAVYHSLLHLDEAPVISTRSDLDTATYVLSQFPTAESRVALIDSV 66
Query: 73 TSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTA 132
TS RVTYGQLS SIRSLACGLYHALGVRKGDVVFVLSAN VLYPVICLAVLSIGAVITTA
Sbjct: 67 TSLRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVLSIGAVITTA 126
Query: 133 NPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDILSVEELIESCI 192
NP+NT SEIGKQVRDSGAKLAVSAPEEL K+SPTGIPTILTTR YGD LSVEELIESC
Sbjct: 127 NPMNTESEIGKQVRDSGAKLAVSAPEELQKLSPTGIPTILTTRSSYGDALSVEELIESCS 186
Query: 193 E-SFEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDV 252
E S EPLPK E++ SDTAAILYSSGTTGTSKGVVLTHSNLI V+E+L W VD++SSQ+DV
Sbjct: 187 ESSSEPLPKAEVTPSDTAAILYSSGTTGTSKGVVLTHSNLISVIEILTWCVDSTSSQHDV 246
Query: 253 FLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGL 312
FLCFIPMFHIYGLVFFGLGLF RGITTVLM RFNFQSMIDAIEKYK+NNIPAVPPVILGL
Sbjct: 247 FLCFIPMFHIYGLVFFGLGLFRRGITTVLMPRFNFQSMIDAIEKYKINNIPAVPPVILGL 306
Query: 313 VKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDKD 372
VKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAAT I DKD
Sbjct: 307 VKSDGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMITDKD 366
Query: 373 AKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDK 432
AKAHPGSCGMLM F KIVDVETGEGLPPMK+GELWLKSPTIMKEYLGN+EATEAT+D+
Sbjct: 367 AKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYLGNREATEATIDE 426
Query: 433 EGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMED 492
EGWLKTGDL YIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH EI+DAAVIP+ED
Sbjct: 427 EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTEILDAAVIPIED 486
Query: 493 EAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDL 552
EAAGQIPVACVVKA +C+L+E+QVIQFVASQVAPYKKVRGVRF+ AIPRSLAGKILRKDL
Sbjct: 487 EAAGQIPVACVVKAPSCELSEQQVIQFVASQVAPYKKVRGVRFISAIPRSLAGKILRKDL 546
Query: 553 VSQFKQQQLLSKL 565
VSQFKQ QLLSKL
Sbjct: 547 VSQFKQLQLLSKL 559
BLAST of Lag0040461 vs. TAIR 10
Match:
AT1G20510.1 (OPC-8:0 CoA ligase1 )
HSP 1 Score: 448.7 bits (1153), Expect = 6.7e-126
Identity = 244/542 (45.02%), Postives = 347/542 (64.02%), Query Frame = 0
Query: 19 NSSGGY-NVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRV 78
NS G+ N N+ Y I + P LD TF+ SQ R+A ID+ T +
Sbjct: 5 NSRSGFCNSNSTFYSKRTPI----PLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64
Query: 79 TYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNT 138
T+ +L ++ S+A L +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65 TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124
Query: 139 ASEIGKQVRDSGAKLAVSAPEELHKVSPTG--IPTILTTRPPYGDILSVEELIESC-IES 198
++EI KQ++DS LA + + L K+S +P +L + V L+E E
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184
Query: 199 FEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLC 258
K + Q DTA +LYSSGTTG SKGV+ +H NLI +++ ++ + + F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244
Query: 259 FIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKS 318
+PMFHIYGL F GL G T +++ +F M+ AI KY+ ++P VPP+++ +V
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304
Query: 319 G----GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDK 378
DLSS+ + G APL K+V E F EK+P V++ GYGLTESTG T +
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364
Query: 379 DAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLD 438
+++ + G+ G L + +IVD TG+ L P + GELWLK P+IMK Y N+EAT +TLD
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424
Query: 439 KEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPME 498
EGWL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LL+HPEI DAAVIP
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484
Query: 499 DEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKD 553
D+ GQ P+A VV+ + L+E+ +++FVA QVAPYK++R V F+ +IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537
BLAST of Lag0040461 vs. TAIR 10
Match:
AT4G05160.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 432.6 bits (1111), Expect = 5.0e-121
Identity = 235/522 (45.02%), Postives = 332/522 (63.60%), Query Frame = 0
Query: 47 PDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVF 106
P+ +F+ + S++A+ DS T +T+ QL +++ LA G +H LG+RK DVV
Sbjct: 25 PNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG-FHRLGIRKNDVVL 84
Query: 107 VLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPT 166
+ + NS +P+ LAV +IG V TTANP+ T +E+ KQ++DS K+ +S + K+
Sbjct: 85 IFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGF 144
Query: 167 GIPTIL-----TTRPPYGD---ILSVEELIESCIESFEPLPKTEMSQSDTAAILYSSGTT 226
+P +L T P G ILS + ++E E P E+ QSDTAA+LYSSGTT
Sbjct: 145 DLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFVEIKQSDTAALLYSSGTT 204
Query: 227 GTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITT 286
GTSKGV LTH N I ++ D + VFLCF+PMFH++GL RG
Sbjct: 205 GTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNAL 264
Query: 287 VLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKD 346
V M RF + ++ IEK++V ++ VPPV L L K DLSSL+ IGSGAAPLGKD
Sbjct: 265 VSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKD 324
Query: 347 VEEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETG 406
+ E P V L GYG+TE+ G ++ + K + GS GML A+IV VETG
Sbjct: 325 LMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETG 384
Query: 407 EGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIK 466
+ PP ++GE+W++ P +MK YL N +AT+ T+DK+ W+ TGDL Y +EDG +Y+VDRIK
Sbjct: 385 KSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIK 444
Query: 467 ELIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVI 526
ELIK+ G+QVAPAELE +L+SHP+I+DA VIP DE AG++P+A VV++ N +TE+ +
Sbjct: 445 ELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQ 504
Query: 527 QFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFKQQ 559
+F+A QVAPYK++R V F+ +P+S AGKILR++LV Q + +
Sbjct: 505 KFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543
BLAST of Lag0040461 vs. TAIR 10
Match:
AT1G20500.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 429.1 bits (1102), Expect = 5.5e-120
Identity = 230/521 (44.15%), Postives = 332/521 (63.72%), Query Frame = 0
Query: 43 ISTRPDL--DTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVR 102
+S P+L D TF+ SQ + A ID+ T +T+ L ++ +A LYH +G+R
Sbjct: 30 LSLPPNLSRDVTTFISSQ--PHRGKTAFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIR 89
Query: 103 KGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEEL 162
+GDVV +LS NS+ PV+CL+V+S+GAV TTAN +NT+ EI KQ+ DS L V +L
Sbjct: 90 RGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIADSNPTL-VFTTRQL 149
Query: 163 HKVSPTGIPTILTTRPPYGDILSVEELIESCIESFEPLP-----KTEMSQSDTAAILYSS 222
P I +LT Y ++ S ++ E + P + ++Q DTA +LYSS
Sbjct: 150 APKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSS 209
Query: 223 GTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRG 282
GTTG SKGV+ +H NL + ++ + ++D+F+C +PMFH YGL+ F +G G
Sbjct: 210 GTTGPSKGVISSHRNLTAHVAR---FISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALG 269
Query: 283 ITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAA 342
T V+++RF M+DA+EK++ + PPV++ ++ DLSSL+ + G A
Sbjct: 270 STVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGA 329
Query: 343 PLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIV 402
PL K+V E F EK+P V++ GY LTES G T ++++ + G+ G L S A+IV
Sbjct: 330 PLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRY-GTAGTLTSDVEARIV 389
Query: 403 DVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYI 462
D TG + + GELWLK P+I K Y N+EAT T++ EGWLKTGDL YIDEDGF+++
Sbjct: 390 DPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFV 449
Query: 463 VDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLT 522
VDR+KELIK+ GYQV PAELE +L++HP+I+DAAVIP D+ AGQ P+A VV+ L+
Sbjct: 450 VDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLS 509
Query: 523 EEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
E+QVI F++ QVAPYKK+R V F+++IP++ +GK LRKDL+
Sbjct: 510 EKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 543
BLAST of Lag0040461 vs. TAIR 10
Match:
AT1G20480.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 416.8 bits (1070), Expect = 2.8e-116
Identity = 237/543 (43.65%), Postives = 344/543 (63.35%), Query Frame = 0
Query: 23 GYNVNTAVYHSLVQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQL 82
G+ +T++++S E + LD +F+ SQ + +D+VT R+++ +L
Sbjct: 26 GFCESTSIFYSK---REPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPEL 85
Query: 83 SASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 142
+ +A G +ALGVRKG+VV +LS NS+L+P++ L+V+S+GA+ITTANP+NT+ EI
Sbjct: 86 WLGVERVA-GCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 145
Query: 143 KQVRDSGAKLAVSAPEELHKV---SPTGIPTIL-----TTRPPYGDILSVEELIESCIES 202
KQ+ DS LA + + + K+ S +P +L YGD + + +E+ IE+
Sbjct: 146 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 205
Query: 203 --FEPLPKTEMSQSDTAAILYSSGTTGTSKGVVLTHSNLICVMELLIWYVDASSSQNDVF 262
E K ++Q DTAA+LYSSGTTGTSKGV+L+H NLI L+ A
Sbjct: 206 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA----LVQAYRARFGLEQRT 265
Query: 263 LCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLV 322
+C IPM HI+G F GL G T V++ +F+ ++ A+E ++ + + VPP+++ +V
Sbjct: 266 ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 325
Query: 323 KS----GGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIK 382
DLSSL + +G APL ++V E F E +P V++ GYGLTEST A
Sbjct: 326 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 385
Query: 383 DKDAKAHPGSCGMLMSRFSAKIVDVETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEAT 442
++ K + G+ G+L KIVD +TG L + GELW++SPT+MK Y NKEAT +T
Sbjct: 386 KEETKRY-GASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAST 445
Query: 443 LDKEGWLKTGDLSYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIP 502
+D EGWLKTGDL YID DGFV++VDR+KELIK NGYQVAPAELE +LL+HPEI DAAVIP
Sbjct: 446 IDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIP 505
Query: 503 MEDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILR 552
+ D AGQ P+A +V+ L+E +++ FVA QV+PYKK+R V F+ +IP++ +GKILR
Sbjct: 506 IPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILR 557
BLAST of Lag0040461 vs. TAIR 10
Match:
AT4G19010.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 413.7 bits (1062), Expect = 2.4e-115
Identity = 225/514 (43.77%), Postives = 333/514 (64.79%), Query Frame = 0
Query: 47 PDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVF 106
P+LD + + S ++ ALIDS+T +++ +L ++S+A G+YH LGVR+GDVV
Sbjct: 45 PNLDAVSALFSHKHHGDT--ALIDSLTGFSISHTELQIMVQSMAAGIYHVLGVRQGDVVS 104
Query: 107 VLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPT 166
++ NSV +P+I L+++S+GA++TT NP ++ EI KQV + LA ++ E + K+S
Sbjct: 105 LVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSL 164
Query: 167 GIPTILTTRPPYGDILSVE--ELIESCIESFEPLPKTEMSQSDTAAILYSSGTTGTSKGV 226
G+ I + D + +E + ESF +PK + Q D AAI+YSSGTTG SKGV
Sbjct: 165 GVSVISVSESYDFDSIRIENPKFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGV 224
Query: 227 VLTHSNLICVMELLIWYVDASS----SQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVL 286
+LTH NLI MEL + + +AS ++V+L +P+ HIYGL F +GL G T V+
Sbjct: 225 LLTHRNLIASMELFVRF-EASQYEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVV 284
Query: 287 MQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSGG---GSDLSSLRRIGSGAAPLGKDV 346
M+RF+ +++ IE++K+ + P VPP+++ L K G SL+++ SGAAPL +
Sbjct: 285 MKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKF 344
Query: 347 EEAFREKFPWVELRPGYGLTESTGAATITIKDKDAKAHPGSCGMLMSRFSAKIVDVETGE 406
E F + P V+L GYG+TEST T + + S G+L AK+VD +G
Sbjct: 345 IEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRY-SSVGLLAPNMQAKVVDWSSGS 404
Query: 407 GLPPMKEGELWLKSPTIMKEYLGNKEATEATLDKEGWLKTGDLSYIDEDGFVYIVDRIKE 466
LPP GELW++ P +MK YL N +AT+ ++ ++ WL+TGD++Y DEDG+++IVDRIKE
Sbjct: 405 FLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKE 464
Query: 467 LIKHNGYQVAPAELETILLSHPEIIDAAVIPMEDEAAGQIPVACVVKASNCQLTEEQVIQ 526
+IK+ G+Q+APA+LE +L+SHP IIDAAV +E G+IPVA VV+ L+EE VI
Sbjct: 465 IIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVIS 524
Query: 527 FVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDL 552
+VASQVAPY+KVR V +++IP+S GKILRK+L
Sbjct: 525 YVASQVAPYRKVRKVVMVNSIPKSPTGKILRKEL 554
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022994494.1 | 1.8e-282 | 89.36 | 4-coumarate--CoA ligase-like 5 [Cucurbita maxima] | [more] |
| KAG7031989.1 | 9.0e-282 | 89.54 | 4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| XP_022957594.1 | 5.8e-281 | 89.36 | 4-coumarate--CoA ligase 1-like [Cucurbita moschata] | [more] |
| XP_038891768.1 | 4.9e-280 | 89.01 | 4-coumarate--CoA ligase-like 5 [Benincasa hispida] | [more] |
| KAG6601192.1 | 4.9e-280 | 89.18 | 4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| M4IQR7 | 2.2e-126 | 45.45 | Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1 | [more] |
| Q84P21 | 9.4e-125 | 45.02 | 4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... | [more] |
| Q9M0X9 | 7.0e-120 | 45.02 | 4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... | [more] |
| P0C5B6 | 7.7e-119 | 44.15 | OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1 | [more] |
| M4IRL6 | 2.1e-116 | 43.93 | Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1K303 | 8.8e-283 | 89.36 | 4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ... | [more] |
| A0A6J1H2E6 | 2.8e-281 | 89.36 | 4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=... | [more] |
| A0A6J1CEK1 | 1.9e-277 | 87.57 | 4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE... | [more] |
| A0A1S3BFZ3 | 1.9e-277 | 88.34 | 4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348942... | [more] |
| A0A0A0KRI4 | 1.9e-269 | 87.70 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1 | [more] |
