Lag0040400 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040400 vs. NCBI nr
Match: KAG6579374.1 (hypothetical protein SDJN03_23822, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.7 bits (901), Expect = 4.8e-93
Identity = 178/259 (68.73%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS+FAP SLLHSY+RSFNGFV KLTEVE  KVS                     
Sbjct: 52  MLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFLNGKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPPPKWKG+CE S +FSC
Sbjct: 112 WDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPPKWKGSCEVSSDFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT  ASTVAG LVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGELVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSDGC DAD+LAAFDDAIADGVDI+S S+GGR  +DYFNDSIAIGAFHA
Sbjct: 232 PSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHA 291

BLAST of Lag0040400 vs. NCBI nr
Match: XP_023550545.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.3 bits (900), Expect = 6.2e-93
Identity = 178/259 (68.73%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS+FAP SLLHSY+RSFNGFV KLTEVE  KVS                     
Sbjct: 52  MLQETIGSSFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPP KWKG+CE S +FSC
Sbjct: 112 WDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT  ASTVAGGLVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSDGC DAD+LAAFDDAIADGVDI+S S+GGR  +DYFNDSIAIGAFHA
Sbjct: 232 PSARIASYKICWSDGCRDADVLAAFDDAIADGVDIISFSVGGRKPRDYFNDSIAIGAFHA 291

BLAST of Lag0040400 vs. NCBI nr
Match: XP_022922167.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 1.1e-92
Identity = 178/259 (68.73%), Postives = 200/259 (77.22%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS+FAP SLLHSY+RSFNGFVVKLTE E   +S                     
Sbjct: 52  MLQETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPPP+WKG+CEAS NFSC
Sbjct: 112 WDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT TASTVAGGLVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSD C DADILAAFDDAIADGVDI+S S+GG   KDYFNDS+AIGAFHA
Sbjct: 232 PSARIASYKICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHA 291

BLAST of Lag0040400 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 346.3 bits (887), Expect = 2.0e-91
Identity = 177/259 (68.34%), Postives = 197/259 (76.06%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS FAP SLLHSY+RSFNGFV KLTEVE  KVS                     
Sbjct: 52  MLQETIGSHFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPP KWKG+CE S +FSC
Sbjct: 112 WDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT  ASTVAGGLVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSDGC DADILAAFDDAIADGVDI+S S+GG+  +DYFNDSIAIGAFHA
Sbjct: 232 PSARIASYKICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHA 291

BLAST of Lag0040400 vs. NCBI nr
Match: XP_038891432.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 345.9 bits (886), Expect = 2.6e-91
Identity = 176/256 (68.75%), Postives = 195/256 (76.17%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+E IGSTFAP SLLHSY RSFNGFV KLTE EA KVS                     
Sbjct: 54  LLKETIGSTFAPHSLLHSYTRSFNGFVAKLTEPEAKKVSEMEGVISVFPNGKKQLHTTRS 113

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +SN+IVGVLD+GIWPESPSF+  GYGPPPPKWKG+CE S NFSC
Sbjct: 114 WDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGPPPPKWKGSCEVSANFSC 173

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIG R+YRSDGK P  DI GPRDS+GHGT TAS VAGG+VR+ASMLGLGLGTARGGV
Sbjct: 174 NNKIIGGRAYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGVVRQASMLGLGLGTARGGV 233

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 220
           PS+RIA+YKVCWSDGC DADILAAFDDAIADGVDI+S SLGG  ++DYFNDSIAIG+FHA
Sbjct: 234 PSARIASYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSGIRDYFNDSIAIGSFHA 293

BLAST of Lag0040400 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.9e-84
Identity = 157/259 (60.62%), Postives = 186/259 (71.81%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           MLE+V+GSTFAP+S+LH+YKRSFNGF VKLTE EA K++                     
Sbjct: 55  MLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRS 114

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +SNI+VGVLDTGIWPESPSF+D+G+ PPPPKWKGTCE S NF C
Sbjct: 115 WDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRC 174

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
             KIIGARSY         D+ GPRD+NGHGT TAST AGGLV +A++ GLGLGTARGGV
Sbjct: 175 NRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGV 234

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           P +RIA YKVCW+DGC D DILAA+DDAIADGVDI+S+S+GG   + YF D+IAIG+FHA
Sbjct: 235 PLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHA 294

BLAST of Lag0040400 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 7.7e-62
Identity = 128/258 (49.61%), Postives = 155/258 (60.08%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+EV G +     L+ SYKRSFNGF  +LTE E  +V+                     
Sbjct: 58  ILQEVTGESSVEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTAS 117

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+G +D+GIWPES SF+DKG+GPPP KWKG C A  NF
Sbjct: 118 WDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNF 177

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y ++         G RD  GHGT TAST AG  V+  S  G+G GTARG
Sbjct: 178 TCNNKLIGARDYTNE---------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARG 237

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 220
           GVP+SRIA YK C   GC    +L+AFDDAIADGVD++SISLG  +V+ Y  D IAIGAF
Sbjct: 238 GVPASRIAAYKACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAF 297

BLAST of Lag0040400 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-60
Identity = 127/258 (49.22%), Postives = 153/258 (59.30%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+EV G +     L+ SYKRSFNGF   LTE E   V+                     
Sbjct: 56  ILQEVTGESSIEGRLVRSYKRSFNGFSALLTESEREGVAEMEGVVSVFRSKNYKLQTTAS 115

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+G +D+GIWPES SF+DKG+GPPP KWKG C+   NF
Sbjct: 116 WDFMGMKEGKNTKRNFAVESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNF 175

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD  GHGT T ST AG  V   S  G+G GTARG
Sbjct: 176 TCNNKLIGARDYTSE---------GTRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARG 235

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 220
           GVP+SR+A YKVC   GC D ++L+AFDDAIADGVD++S+SLGG     Y  D+IAIGAF
Sbjct: 236 GVPASRVAAYKVCTITGCSDDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAF 295

BLAST of Lag0040400 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-60
Identity = 125/259 (48.26%), Postives = 155/259 (59.85%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVSK-------------------- 60
           +L+EV G +     L+ SYKRSFNGF  +LTE E  +V+K                    
Sbjct: 54  ILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTS 113

Query: 61  -------------------SNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                              S+ I+GV+D+GI PES SF+DKG+GPPP KWKG C    NF
Sbjct: 114 WDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNF 173

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD +GHGT TAST AG  V  AS  G+G GT RG
Sbjct: 174 TCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRG 233

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 221
           GVP+SR+A YKVC   GC    +L+AFDDAIADGVD+++IS+G +    + ND IAIGAF
Sbjct: 234 GVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAF 293

BLAST of Lag0040400 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.5e-60
Identity = 123/259 (47.49%), Postives = 156/259 (60.23%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L++V G +     L+ SYKRSFNGF  +LTE E   ++                     
Sbjct: 55  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 114

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+GV+DTGIWPES SF+DKG+GPPP KWKG C    NF
Sbjct: 115 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 174

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD++GHGT TAST AG  V+  S  G+G GT RG
Sbjct: 175 TCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 234

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 221
           GVP+SRIA YKVC   GC    +L++FDDAIADGVD+++IS+G +    + +D IAIGAF
Sbjct: 235 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 294

BLAST of Lag0040400 vs. ExPASy TrEMBL
Match: A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.1e-93
Identity = 178/259 (68.73%), Postives = 200/259 (77.22%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS+FAP SLLHSY+RSFNGFVVKLTE E   +S                     
Sbjct: 52  MLQETIGSSFAPHSLLHSYQRSFNGFVVKLTEDEVKIISEMKGVISVFPNEKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPPP+WKG+CEAS NFSC
Sbjct: 112 WDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFLDEGYGPPPPRWKGSCEASLNFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT TASTVAGGLVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHTASTVAGGLVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSD C DADILAAFDDAIADGVDI+S S+GG   KDYFNDS+AIGAFHA
Sbjct: 232 PSARIASYKICWSDDCSDADILAAFDDAIADGVDIISFSVGGHTPKDYFNDSMAIGAFHA 291

BLAST of Lag0040400 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.7e-92
Identity = 177/259 (68.34%), Postives = 197/259 (76.06%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           ML+E IGS FAP SLLHSY+RSFNGFV KLTEVE  KVS                     
Sbjct: 52  MLQETIGSHFAPHSLLHSYERSFNGFVAKLTEVEVQKVSEMKGVISVFPNGKKQLHTTRS 111

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+IIVGVLDTGIWPESPSF D+GYGPPP KWKG+CE S +FSC
Sbjct: 112 WDFMGLSQQASRVPSVESDIIVGVLDTGIWPESPSFLDQGYGPPPRKWKGSCEVSSDFSC 171

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYR++G+ P  DI GPRDSNGHGT  ASTVAGGLVR+ASMLGLG GTARGGV
Sbjct: 172 NNKIIGARSYRTNGQYPINDIQGPRDSNGHGTHAASTVAGGLVRQASMLGLGSGTARGGV 231

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA+YK+CWSDGC DADILAAFDDAIADGVDI+S S+GG+  +DYFNDSIAIGAFHA
Sbjct: 232 PSARIASYKICWSDGCPDADILAAFDDAIADGVDIISFSVGGQTPRDYFNDSIAIGAFHA 291

BLAST of Lag0040400 vs. ExPASy TrEMBL
Match: A0A5D3CCZ5 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001930 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.0e-89
Identity = 173/256 (67.58%), Postives = 193/256 (75.39%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+E IGS+F P SLLHS+KRSFNGFV KLTE EA KVS                     
Sbjct: 54  LLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRS 113

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +SN+IVGVLD+GIWPESPSF+  GYGPPP KWKG+CE S NFSC
Sbjct: 114 WDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSC 173

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYRS+G+ P  DI GPRDS+GHGT TAS VAGGLVR+A+MLGLGLGTARGGV
Sbjct: 174 NNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGGV 233

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 220
           PS+RIA YKVCWSDGC DADILAAFDDAIADGVDI+S SLGG   +DYFNDSIAIG+FHA
Sbjct: 234 PSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHA 293

BLAST of Lag0040400 vs. ExPASy TrEMBL
Match: A0A5A7SZX9 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006680 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.0e-89
Identity = 173/256 (67.58%), Postives = 193/256 (75.39%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+E IGS+F P SLLHS+KRSFNGFV KLTE EA KVS                     
Sbjct: 54  LLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRS 113

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +SN+IVGVLD+GIWPESPSF+  GYGPPP KWKG+CE S NFSC
Sbjct: 114 WDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSC 173

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NKIIGARSYRS+G+ P  DI GPRDS+GHGT TAS VAGGLVR+A+MLGLGLGTARGGV
Sbjct: 174 NNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGGV 233

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 220
           PS+RIA YKVCWSDGC DADILAAFDDAIADGVDI+S SLGG   +DYFNDSIAIG+FHA
Sbjct: 234 PSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHA 293

BLAST of Lag0040400 vs. ExPASy TrEMBL
Match: A0A0A0KLR4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.0e-89
Identity = 171/259 (66.02%), Postives = 194/259 (74.90%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           MLEEV+GS+FAP++LLHSYKRSFNGFVVKLTE EA K+S                     
Sbjct: 18  MLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRS 77

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +SNI+VGVLD+GIWPESPSF+D GYGPPPPKWKG C+ S NF C
Sbjct: 78  WDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQTSANFHC 137

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
             KIIGAR+YRSD   P +DI  PRDS+GHGT TASTVAGGLV +AS+ GL LGTARGGV
Sbjct: 138 NRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGV 197

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 223
           PS+RIA YK+CWSDGC+DADILAAFDDAIADGVDI+S+S+GG   K YFNDSIAIGAFH+
Sbjct: 198 PSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHS 257

BLAST of Lag0040400 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 5.5e-63
Identity = 128/258 (49.61%), Postives = 155/258 (60.08%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L+EV G +     L+ SYKRSFNGF  +LTE E  +V+                     
Sbjct: 58  ILQEVTGESSVEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTAS 117

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+G +D+GIWPES SF+DKG+GPPP KWKG C A  NF
Sbjct: 118 WDFLGLKEGKNTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNF 177

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y ++         G RD  GHGT TAST AG  V+  S  G+G GTARG
Sbjct: 178 TCNNKLIGARDYTNE---------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARG 237

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 220
           GVP+SRIA YK C   GC    +L+AFDDAIADGVD++SISLG  +V+ Y  D IAIGAF
Sbjct: 238 GVPASRIAAYKACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAF 297

BLAST of Lag0040400 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 233.4 bits (594), Expect = 1.8e-61
Identity = 125/259 (48.26%), Postives = 155/259 (59.85%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVSK-------------------- 60
           +L+EV G +     L+ SYKRSFNGF  +LTE E  +V+K                    
Sbjct: 54  ILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTS 113

Query: 61  -------------------SNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                              S+ I+GV+D+GI PES SF+DKG+GPPP KWKG C    NF
Sbjct: 114 WDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNF 173

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD +GHGT TAST AG  V  AS  G+G GT RG
Sbjct: 174 TCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRG 233

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 221
           GVP+SR+A YKVC   GC    +L+AFDDAIADGVD+++IS+G +    + ND IAIGAF
Sbjct: 234 GVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAF 293

BLAST of Lag0040400 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 233.0 bits (593), Expect = 2.3e-61
Identity = 123/257 (47.86%), Postives = 156/257 (60.70%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L++V G +     L+ SYKRSFNGF  +LTE E   ++                     
Sbjct: 55  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEGVVSVFPNKILQLHTTTSWD 114

Query: 61  ----------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNFSC 120
                           +S+ I+GV+DTGIWPES SF+DKG+GPPP KWKG C    NF+C
Sbjct: 115 FMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTC 174

Query: 121 INKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARGGV 180
            NK+IGAR Y S+         G RD++GHGT TAST AG  V+  S  G+G GT RGGV
Sbjct: 175 NNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGV 234

Query: 181 PSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAFHA 221
           P+SRIA YKVC   GC    +L++FDDAIADGVD+++IS+G +    + +D IAIGAFHA
Sbjct: 235 PASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHA 294

BLAST of Lag0040400 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 232.3 bits (591), Expect = 3.9e-61
Identity = 123/259 (47.49%), Postives = 156/259 (60.23%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L++V G +     L+ SYKRSFNGF  +LTE E   ++                     
Sbjct: 55  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 114

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+GV+DTGIWPES SF+DKG+GPPP KWKG C    NF
Sbjct: 115 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 174

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD++GHGT TAST AG  V+  S  G+G GT RG
Sbjct: 175 TCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 234

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 221
           GVP+SRIA YKVC   GC    +L++FDDAIADGVD+++IS+G +    + +D IAIGAF
Sbjct: 235 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 294

BLAST of Lag0040400 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 232.3 bits (591), Expect = 3.9e-61
Identity = 123/259 (47.49%), Postives = 156/259 (60.23%), Query Frame = 0

Query: 1   MLEEVIGSTFAPQSLLHSYKRSFNGFVVKLTEVEAHKVS--------------------- 60
           +L++V G +     L+ SYKRSFNGF  +LTE E   ++                     
Sbjct: 55  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 114

Query: 61  ------------------KSNIIVGVLDTGIWPESPSFNDKGYGPPPPKWKGTCEASPNF 120
                             +S+ I+GV+DTGIWPES SF+DKG+GPPP KWKG C    NF
Sbjct: 115 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 174

Query: 121 SCINKIIGARSYRSDGKCPTKDIAGPRDSNGHGTQTASTVAGGLVRRASMLGLGLGTARG 180
           +C NK+IGAR Y S+         G RD++GHGT TAST AG  V+  S  G+G GT RG
Sbjct: 175 TCNNKLIGARDYTSE---------GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 234

Query: 181 GVPSSRIATYKVCWSDGCFDADILAAFDDAIADGVDILSISLGGRVVKDYFNDSIAIGAF 221
           GVP+SRIA YKVC   GC    +L++FDDAIADGVD+++IS+G +    + +D IAIGAF
Sbjct: 235 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 294