Lag0040374 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040374
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLipoyl(octanoyl) transferase
Locationchr13: 4280952 .. 4282786 (-)
RNA-Seq ExpressionLag0040374
SyntenyLag0040374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATACTGTCAACGCCCTCTGCCAGCTTTCTTCCAAGCCGCCCACATACCCGCCGTCGGCCGGAATCACAACGGTCTCAACCTCCAAAGGTATTCTCTAAATTCGATCGAATTCTTCACCCAAAAGCTTCCAGATCAACCTTGATGCTCAATGCACAACAAAGAACGTAAGCTTTTCTTTCACGCATTTTCACAAACGGGTTATGCGCACAATCTGTGGAATCAACCTATAGCTCTGGCTTGGGATGTTGCAGGTGCGAGTGTTTCGATTTGCATAAGGATCTGATTCCATATGGCGATGCATGGTCTTGGCAGAAGGACATTGTGAGAGAAAAAATTGCATTGATCGATGCGAACGAGGACTGCCCAGATTCGCTCATCGTCTTGCAGCATCATCCTGTGTACACAATGGGTACTAACAGTACGGAAGAGTATCTCAATTTTTCTCCTAAGGATTCTCCCTTTTCGATTTATCGCACTGAGCGTGGAGGGGAGGTTACTTATCATGGCCCTGGCCAGGTACTCGATAATTGTTTCTACTTTCTAATCATTGACCTAAGGATTTGATATGATATGAACTTTTTTAGCATTTTCTTATCATTCTAAGTAGGGATATATTGCATTTACTGGGTAGTTTTTGTTTTAATCACTTCACATTGAACTTAGAGTATGTACATATTTTTCTCATATCAATTTTAGCTGTATTCTTCACATTGTAATGGTGATGCTTTGTTTTTCCCTATGTTGTGGTAATTTAGGAGTTCAATCTCAGAGTTGCTGGTTTGGTTGTTTTAGAGGGTTGTTTACTAGTTGCTGGGGTTTCTTCTTGACATTGATAGGATTTGTCCTGTGCTGTAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCGTCATACGTGTTCTTTCCTCGACGTTTTCGATCAACGCTCATCGTGTCGATGGTTTAACGGGTGTCTGGTCAGGTATTCTTTATAGCTCTTGGCCTCAAATTTTTATGTAGGATATTACAAATGAACATCTTTTTCTTCTGTTTTTGTTTGGAAATCTCATTTTTTTTTTAATTTCTTCTCTTTAGAAGTTATATGAGTATGGAATGTTCATTGCAACTTAATCTGACTGAAATTTTACTTGTGTTGTTTAAGTGGCAATGTCAACATTTTTGTTGTCGAGTCATTAGAACTTAAGTTCACTCGGAGTTTTATTATTTACTTCATTATCAACTTGAAATCATGCCTCATATTAATAACATAGCTTCGCTTGGTCCGATGAATTCGAGCACGGGCTTTCATGTTTCATGTCAGTAGTTTCTATAAATTTCCCCCCATCTAGCATGCTATATTCAGTAGTTCCGAATTGGACATTTTTACCTTTCTTTTCTTCAAAACATTGTCAATTTCTTGAATGATTTCCCTCCGTTTTCCCTTTGCAGGAAACCAAAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAACATTTCATGGATTAGCACTTAATGTCACCACTGATCTAACTCCATTTAATTGGATAGTTCCATGTGGAATACGAAATCGCCACGTCGGAAACATCAAGGAGTTGCTGAGAGAAGCTGATTTACACACTGAATTTCAGAACAACATACATCTTGACAATGATTGCCAGTTGATTGATATCACTTTTGAAGCTCTAATACAGGAATTCTCTGAAGTTTTCCAACTTGACATACATCAGAAAACCATCCCCACTTTGGAGTTCATGAAGCCAGCATCCAAAAGCTCCCCTGGTGAGATATCTGATTATCTGTAA

mRNA sequence

ATGATACTGTCAACGCCCTCTGCCAGCTTTCTTCCAAGCCGCCCACATACCCGCCGTCGGCCGGAATCACAACGGTCTCAACCTCCAAAGGTATTCTCTAAATTCGATCGAATTCTTCACCCAAAAGCTTCCAGATCAACCTTGATGCTCAATGCACAACAAAGAACGTGCGAGTGTTTCGATTTGCATAAGGATCTGATTCCATATGGCGATGCATGGTCTTGGCAGAAGGACATTGTGAGAGAAAAAATTGCATTGATCGATGCGAACGAGGACTGCCCAGATTCGCTCATCGTCTTGCAGCATCATCCTGTGTACACAATGGGTACTAACAGTACGGAAGAGTATCTCAATTTTTCTCCTAAGGATTCTCCCTTTTCGATTTATCGCACTGAGCGTGGAGGGGAGGTTACTTATCATGGCCCTGGCCAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCGTCATACGTGTTCTTTCCTCGACGTTTTCGATCAACGCTCATCGTGTCGATGGTTTAACGGGTGTCTGGTCAGGAAACCAAAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAACATTTCATGGATTAGCACTTAATGTCACCACTGATCTAACTCCATTTAATTGGATAGTTCCATGTGGAATACGAAATCGCCACGTCGGAAACATCAAGGAGTTGCTGAGAGAAGCTGATTTACACACTGAATTTCAGAACAACATACATCTTGACAATGATTGCCAGTTGATTGATATCACTTTTGAAGCTCTAATACAGGAATTCTCTGAAGTTTTCCAACTTGACATACATCAGAAAACCATCCCCACTTTGGAGTTCATGAAGCCAGCATCCAAAAGCTCCCCTGGTGAGATATCTGATTATCTGTAA

Coding sequence (CDS)

ATGATACTGTCAACGCCCTCTGCCAGCTTTCTTCCAAGCCGCCCACATACCCGCCGTCGGCCGGAATCACAACGGTCTCAACCTCCAAAGGTATTCTCTAAATTCGATCGAATTCTTCACCCAAAAGCTTCCAGATCAACCTTGATGCTCAATGCACAACAAAGAACGTGCGAGTGTTTCGATTTGCATAAGGATCTGATTCCATATGGCGATGCATGGTCTTGGCAGAAGGACATTGTGAGAGAAAAAATTGCATTGATCGATGCGAACGAGGACTGCCCAGATTCGCTCATCGTCTTGCAGCATCATCCTGTGTACACAATGGGTACTAACAGTACGGAAGAGTATCTCAATTTTTCTCCTAAGGATTCTCCCTTTTCGATTTATCGCACTGAGCGTGGAGGGGAGGTTACTTATCATGGCCCTGGCCAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCGTCATACGTGTTCTTTCCTCGACGTTTTCGATCAACGCTCATCGTGTCGATGGTTTAACGGGTGTCTGGTCAGGAAACCAAAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAACATTTCATGGATTAGCACTTAATGTCACCACTGATCTAACTCCATTTAATTGGATAGTTCCATGTGGAATACGAAATCGCCACGTCGGAAACATCAAGGAGTTGCTGAGAGAAGCTGATTTACACACTGAATTTCAGAACAACATACATCTTGACAATGATTGCCAGTTGATTGATATCACTTTTGAAGCTCTAATACAGGAATTCTCTGAAGTTTTCCAACTTGACATACATCAGAAAACCATCCCCACTTTGGAGTTCATGAAGCCAGCATCCAAAAGCTCCCCTGGTGAGATATCTGATTATCTGTAA

Protein sequence

MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKPASKSSPGEISDYL
Homology
BLAST of Lag0040374 vs. NCBI nr
Match: XP_022150592.1 (plastidial lipoyltransferase 2-like [Momordica charantia] >XP_022150593.1 plastidial lipoyltransferase 2-like [Momordica charantia])

HSP 1 Score: 527.3 bits (1357), Expect = 8.8e-146
Identity = 255/305 (83.61%), Postives = 274/305 (89.84%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           +++STPSA  LP       RPE QRS+P KV S+ DRILHPKAS S + LN+++RTCECF
Sbjct: 2   ILVSTPSAGLLPC------RPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH DLIPYGDAW+WQKDIVREK ALIDANE+CPD+LIVLQHHPVYTMGT+S+EEYLNF 
Sbjct: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
           PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLE-FMK 300
           N+KELLRE DLHT FQ NIHLD D QLID TFEAL+QEFSEVFQLDIHQKTIPTLE F+ 
Sbjct: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300

Query: 301 PASKS 305
            A KS
Sbjct: 302 QAPKS 300

BLAST of Lag0040374 vs. NCBI nr
Match: KAA0034631.1 (plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa] >TYK09182.1 plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 252/310 (81.29%), Postives = 271/310 (87.42%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MIL TPSA FLPS  +T R PESQRS P K+F KFD+IL  K S S L LNA  ++CECF
Sbjct: 192 MILLTPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCECF 251

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEY NF 
Sbjct: 252 DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNFD 311

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
            KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 312 LKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTFS 371

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 372 INAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKVG 431

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITF ALIQEFSEVFQLD++ KTIP+LEF+K 
Sbjct: 432 NIKELLREVDLHTGFQ------KDSQLIDITFGALIQEFSEVFQLDMYHKTIPSLEFLKQ 491

Query: 301 ASKSSPGEIS 311
              +SPGEIS
Sbjct: 492 IPNNSPGEIS 495

BLAST of Lag0040374 vs. NCBI nr
Match: XP_016900264.1 (PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 252/310 (81.29%), Postives = 271/310 (87.42%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MIL TPSA FLPS  +T R PESQRS P K+F KFD+IL  K S S L LNA  ++CECF
Sbjct: 1   MILLTPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCECF 60

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEY NF 
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNFD 120

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
            KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKVG 240

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITF ALIQEFSEVFQLD++ KTIP+LEF+K 
Sbjct: 241 NIKELLREVDLHTGFQ------KDSQLIDITFGALIQEFSEVFQLDMYHKTIPSLEFLKQ 300

Query: 301 ASKSSPGEIS 311
              +SPGEIS
Sbjct: 301 IPNNSPGEIS 304

BLAST of Lag0040374 vs. NCBI nr
Match: XP_004135053.2 (octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158.1 hypothetical protein Csa_008925 [Cucumis sativus])

HSP 1 Score: 517.3 bits (1331), Expect = 9.2e-143
Identity = 250/312 (80.13%), Postives = 270/312 (86.54%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MILSTPSA FLPSR  T R PESQRS P  +  KFD+ILH K S S+L LNA+ ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEYLNF 
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGI+NR VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITFEALIQEFSEVFQLDI+ KTIP+LEF+K 
Sbjct: 241 NIKELLREVDLHTGFQ------KDSQLIDITFEALIQEFSEVFQLDIYHKTIPSLEFLKQ 300

Query: 301 ASKSSPGEISDY 313
              S P    D+
Sbjct: 301 IPNSPPASSGDH 306

BLAST of Lag0040374 vs. NCBI nr
Match: XP_031742006.1 (octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 516.9 bits (1330), Expect = 1.2e-142
Identity = 250/307 (81.43%), Postives = 269/307 (87.62%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MILSTPSA FLPSR  T R PESQRS P  +  KFD+ILH K S S+L LNA+ ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEYLNF 
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGI+NR VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITFEALIQEFSEVFQLDI+ KTIP+LEF+K 
Sbjct: 241 NIKELLREVDLHTGFQ------KDSQLIDITFEALIQEFSEVFQLDIYHKTIPSLEFLKQ 300

Query: 301 ASKSSPG 308
              S PG
Sbjct: 301 IPNSPPG 301

BLAST of Lag0040374 vs. ExPASy Swiss-Prot
Match: Q948J9 (Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 6.5e-99
Identity = 174/252 (69.05%), Postives = 203/252 (80.56%), Query Frame = 0

Query: 45  RSTLMLNAQQ----RTCECFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVL 104
           RS  +LN+      R C CFDL+  L+PY  AWSWQK IV EK  LID N+DC D++I+L
Sbjct: 29  RSVKILNSGNHEIPRKCLCFDLYDKLVPYKKAWSWQKSIVEEKKTLIDRNQDCADTVILL 88

Query: 105 QHHPVYTMGTNSTEEYLNFSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDL 164
           QH PVYTMGT STE+YLNF  KD+PF++YRTERGGEVTYHGPGQLVMYPIINLRNH+MDL
Sbjct: 89  QHSPVYTMGTASTEDYLNFDIKDAPFNVYRTERGGEVTYHGPGQLVMYPIINLRNHEMDL 148

Query: 165 HWYLRALEEVVIRVLSSTFSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVT 224
           HWYLR LEE+VIRVLSSTFSI A R+DGLTGVW GNQK+AAIGIRVSKWIT+HGLALNVT
Sbjct: 149 HWYLRMLEEIVIRVLSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSKWITYHGLALNVT 208

Query: 225 TDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFS 284
           TDLTPFNWIVPCGIR+R VGNIK LL + +          + +D +LIDI  E+L++EFS
Sbjct: 209 TDLTPFNWIVPCGIRDRKVGNIKGLLEDGE--------HGMVDDLRLIDIVHESLLKEFS 268

Query: 285 EVFQLDIHQKTI 293
           E FQL I ++T+
Sbjct: 269 EAFQLQIEKQTV 272

BLAST of Lag0040374 vs. ExPASy Swiss-Prot
Match: P0C7R2 (Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P2 PE=1 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.6e-97
Identity = 170/259 (65.64%), Postives = 206/259 (79.54%), Query Frame = 0

Query: 42  KASRSTLMLNAQQRT----CECFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSL 101
           + S S  ++N++  T    CECFDL+  LIPY  AWSWQK I+ EK ALID N++C DSL
Sbjct: 28  RVSTSLKIMNSKNHTNPRKCECFDLYDQLIPYKKAWSWQKSILNEKKALIDKNQECSDSL 87

Query: 102 IVLQHHPVYTMGTNSTEEYLNFSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHK 161
           I+LQH  VYTMGT S+E YLNF  K++PF +YRTERGGEVTYHGPGQLVMYPIINLRNHK
Sbjct: 88  IILQHPSVYTMGTGSSENYLNFDIKNAPFDVYRTERGGEVTYHGPGQLVMYPIINLRNHK 147

Query: 162 MDLHWYLRALEEVVIRVLSSTFSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLAL 221
           MDLHWYLR LEEVVIRVLSS F+INA R+DG TGVW GN+K+AAIGIRVSKW+T+HGLAL
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSKWMTYHGLAL 207

Query: 222 NVTTDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQ 281
           NVTTDLTPFN IVPCGIRNR VG++K L+ + + + + +       D QL+DI  E+L++
Sbjct: 208 NVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHYNKLE-------DLQLLDIAHESLLK 267

Query: 282 EFSEVFQLDIHQKTIPTLE 297
           EFSEVFQL + ++T+  LE
Sbjct: 268 EFSEVFQLQMEKQTVFKLE 279

BLAST of Lag0040374 vs. ExPASy Swiss-Prot
Match: Q3M698 (Octanoyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=lipB PE=3 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 1.6e-52
Identity = 98/186 (52.69%), Postives = 131/186 (70.43%), Query Frame = 0

Query: 59  CFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLN 118
           C   +K L+PY +A +WQ+ ++ ++I       +  D LI+L+H PVYT+G  S+ E+L 
Sbjct: 12  CLLYNKGLMPYLEAHAWQRSLLHQRIDY----PNLEDVLILLEHPPVYTLGQGSSLEFLK 71

Query: 119 FSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSST 178
           F P  S F I+R ERGGEVTYH PGQLV YPI+NLR H+ DLHWYLR LEEV+IRVL + 
Sbjct: 72  FDPNQSEFEIHRIERGGEVTYHCPGQLVGYPILNLRRHRQDLHWYLRQLEEVIIRVL-AV 131

Query: 179 FSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRH 238
           + +   R+  LTGVW   +K+AAIGI+VS+WIT HG ALNV  D+T F  IVPCGI ++ 
Sbjct: 132 YGLKGDRLPSLTGVWLEGRKVAAIGIKVSRWITMHGFALNVCPDMTGFGRIVPCGITDKP 191

Query: 239 VGNIKE 245
           VG++ +
Sbjct: 192 VGSLAQ 192

BLAST of Lag0040374 vs. ExPASy Swiss-Prot
Match: Q8YSA4 (Octanoyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=lipB PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.7e-52
Identity = 105/229 (45.85%), Postives = 147/229 (64.19%), Query Frame = 0

Query: 59  CFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLN 118
           C   +K L+PY  A +WQ+ ++R++I       +  D LI+L+H PVYT+G  S+ E+L 
Sbjct: 12  CLLYNKGLMPYLAAHAWQRSLLRQRIDY----PNLEDVLILLEHPPVYTLGQGSSLEFLK 71

Query: 119 FSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSST 178
           F P  S F I+R ERGGEVTYH PGQLV YPI+NL  H+ DLHWYLR LEEV+IRVL + 
Sbjct: 72  FDPNQSEFEIHRIERGGEVTYHCPGQLVGYPILNLHRHRQDLHWYLRQLEEVIIRVL-AV 131

Query: 179 FSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRH 238
           + +   R+  LTGVW   +K+AAIGI+VS+WIT HG ALNV  D+T F+ IVPCGI ++ 
Sbjct: 132 YDLKGDRLPSLTGVWLEGRKVAAIGIKVSRWITMHGFALNVCPDMTGFSRIVPCGITDKP 191

Query: 239 VGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDI 288
           VG++ + +                  C+ + +    ++Q F+EVF L++
Sbjct: 192 VGSLAQWIPGI--------------TCEEVRV---HVVQAFAEVFGLEL 218

BLAST of Lag0040374 vs. ExPASy Swiss-Prot
Match: Q8DKM7 (Octanoyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=lipB PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.8e-51
Identity = 104/230 (45.22%), Postives = 138/230 (60.00%), Query Frame = 0

Query: 66  LIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFSPKDSP 125
           +IPY DAW WQ+ +V E+      N   PD L+ L+H  +YT+G  +T  +++F P  + 
Sbjct: 13  IIPYRDAWRWQQQLVAER----RQNLAAPDVLLTLEHPAIYTLGQGATTAHIHFDPVATG 72

Query: 126 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 185
             + R ERGG+VTYH PGQLV YPI+NLR H  DLHWYL  LEEV+I+ L   + I   R
Sbjct: 73  IEVLRIERGGDVTYHCPGQLVAYPILNLRRHCCDLHWYLHQLEEVIIQTLRH-YGIKGER 132

Query: 186 VDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVGNIKEL 245
           V GLTGVW    K+AAIGI+V++WI+FHG+ALNV  DL  F  IVPCGI +R VGN+++ 
Sbjct: 133 VAGLTGVWVEGYKVAAIGIKVTRWISFHGIALNVVNDLKGFGRIVPCGIGDRPVGNLRQF 192

Query: 246 LREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTL 296
                             D QL D+  +  +  FS VF L+   K +  L
Sbjct: 193 CA----------------DIQLEDVR-QQFVAAFSRVFGLEFAPKALADL 220

BLAST of Lag0040374 vs. ExPASy TrEMBL
Match: A0A6J1D9U7 (Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.3e-146
Identity = 255/305 (83.61%), Postives = 274/305 (89.84%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           +++STPSA  LP       RPE QRS+P KV S+ DRILHPKAS S + LN+++RTCECF
Sbjct: 2   ILVSTPSAGLLPC------RPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH DLIPYGDAW+WQKDIVREK ALIDANE+CPD+LIVLQHHPVYTMGT+S+EEYLNF 
Sbjct: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
           PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLE-FMK 300
           N+KELLRE DLHT FQ NIHLD D QLID TFEAL+QEFSEVFQLDIHQKTIPTLE F+ 
Sbjct: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300

Query: 301 PASKS 305
            A KS
Sbjct: 302 QAPKS 300

BLAST of Lag0040374 vs. ExPASy TrEMBL
Match: A0A5D3CD13 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001740 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 252/310 (81.29%), Postives = 271/310 (87.42%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MIL TPSA FLPS  +T R PESQRS P K+F KFD+IL  K S S L LNA  ++CECF
Sbjct: 192 MILLTPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCECF 251

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEY NF 
Sbjct: 252 DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNFD 311

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
            KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 312 LKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTFS 371

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 372 INAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKVG 431

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITF ALIQEFSEVFQLD++ KTIP+LEF+K 
Sbjct: 432 NIKELLREVDLHTGFQ------KDSQLIDITFGALIQEFSEVFQLDMYHKTIPSLEFLKQ 491

Query: 301 ASKSSPGEIS 311
              +SPGEIS
Sbjct: 492 IPNNSPGEIS 495

BLAST of Lag0040374 vs. ExPASy TrEMBL
Match: A0A1S4DWA5 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 252/310 (81.29%), Postives = 271/310 (87.42%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MIL TPSA FLPS  +T R PESQRS P K+F KFD+IL  K S S L LNA  ++CECF
Sbjct: 1   MILLTPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCECF 60

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEY NF 
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNFD 120

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
            KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNR VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKVG 240

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITF ALIQEFSEVFQLD++ KTIP+LEF+K 
Sbjct: 241 NIKELLREVDLHTGFQ------KDSQLIDITFGALIQEFSEVFQLDMYHKTIPSLEFLKQ 300

Query: 301 ASKSSPGEIS 311
              +SPGEIS
Sbjct: 301 IPNNSPGEIS 304

BLAST of Lag0040374 vs. ExPASy TrEMBL
Match: A0A0A0KRC7 (Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.4e-143
Identity = 250/312 (80.13%), Postives = 270/312 (86.54%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MILSTPSA FLPSR  T R PESQRS P  +  KFD+ILH K S S+L LNA+ ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH++LIPYGDAWSWQK+IVREK+ LIDAN+DCPD+LIVLQHHPVYTMGTNSTEEYLNF 
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INAHR+DGLTGVW+GNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGI+NR VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPTLEFMKP 300
           NIKELLRE DLHT FQ       D QLIDITFEALIQEFSEVFQLDI+ KTIP+LEF+K 
Sbjct: 241 NIKELLREVDLHTGFQ------KDSQLIDITFEALIQEFSEVFQLDIYHKTIPSLEFLKQ 300

Query: 301 ASKSSPGEISDY 313
              S P    D+
Sbjct: 301 IPNSPPASSGDH 306

BLAST of Lag0040374 vs. ExPASy TrEMBL
Match: A0A6J1G154 (Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.6e-143
Identity = 255/309 (82.52%), Postives = 273/309 (88.35%), Query Frame = 0

Query: 1   MILSTPSASFLPSRPHTRRRPESQRSQPPKVFSKFDRILHPKASRSTLMLNAQQRTCECF 60
           MILSTPSASF PS   T RRPES  S P KV +KFDR+LHPKAS+S L LNAQQRTCECF
Sbjct: 3   MILSTPSASFFPSCGRTHRRPESPWSHPRKVIAKFDRVLHPKASKSRLTLNAQQRTCECF 62

Query: 61  DLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFS 120
           DLH+DLIPY DAWSWQKDIVREKIALIDANEDCPDSLIVLQH PVYT+GTNSTEE+LNF 
Sbjct: 63  DLHRDLIPYNDAWSWQKDIVREKIALIDANEDCPDSLIVLQHPPVYTLGTNSTEEFLNFD 122

Query: 121 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 180
           PKDSPFSIYRTERGGEVTYHGPGQ+VMYPIINLRNHKMDLHWYLRALEEV+IRVLSSTFS
Sbjct: 123 PKDSPFSIYRTERGGEVTYHGPGQIVMYPIINLRNHKMDLHWYLRALEEVIIRVLSSTFS 182

Query: 181 INAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVG 240
           INA+RVDGLTGVW+GNQKLAAIGIRVSKWITFHGLALNVTTDL+PFN IVPCGIRNR VG
Sbjct: 183 INANRVDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLSPFNLIVPCGIRNRQVG 242

Query: 241 NIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFSEVFQLDIHQKTIPT--LEFM 300
           NIKELL    L  E +N+IH D D QLIDITFEALI +F++VFQ+DIHQKT P   LE +
Sbjct: 243 NIKELLL---LLREAENSIHFDKDSQLIDITFEALIHQFAQVFQVDIHQKTTPPPFLESL 302

Query: 301 KPASKSSPG 308
           K A  SSPG
Sbjct: 303 KQAPPSSPG 308

BLAST of Lag0040374 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 362.1 bits (928), Expect = 4.6e-100
Identity = 174/252 (69.05%), Postives = 203/252 (80.56%), Query Frame = 0

Query: 45  RSTLMLNAQQ----RTCECFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSLIVL 104
           RS  +LN+      R C CFDL+  L+PY  AWSWQK IV EK  LID N+DC D++I+L
Sbjct: 29  RSVKILNSGNHEIPRKCLCFDLYDKLVPYKKAWSWQKSIVEEKKTLIDRNQDCADTVILL 88

Query: 105 QHHPVYTMGTNSTEEYLNFSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDL 164
           QH PVYTMGT STE+YLNF  KD+PF++YRTERGGEVTYHGPGQLVMYPIINLRNH+MDL
Sbjct: 89  QHSPVYTMGTASTEDYLNFDIKDAPFNVYRTERGGEVTYHGPGQLVMYPIINLRNHEMDL 148

Query: 165 HWYLRALEEVVIRVLSSTFSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLALNVT 224
           HWYLR LEE+VIRVLSSTFSI A R+DGLTGVW GNQK+AAIGIRVSKWIT+HGLALNVT
Sbjct: 149 HWYLRMLEEIVIRVLSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSKWITYHGLALNVT 208

Query: 225 TDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQEFS 284
           TDLTPFNWIVPCGIR+R VGNIK LL + +          + +D +LIDI  E+L++EFS
Sbjct: 209 TDLTPFNWIVPCGIRDRKVGNIKGLLEDGE--------HGMVDDLRLIDIVHESLLKEFS 268

Query: 285 EVFQLDIHQKTI 293
           E FQL I ++T+
Sbjct: 269 EAFQLQIEKQTV 272

BLAST of Lag0040374 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 357.5 bits (916), Expect = 1.1e-98
Identity = 170/259 (65.64%), Postives = 206/259 (79.54%), Query Frame = 0

Query: 42  KASRSTLMLNAQQRT----CECFDLHKDLIPYGDAWSWQKDIVREKIALIDANEDCPDSL 101
           + S S  ++N++  T    CECFDL+  LIPY  AWSWQK I+ EK ALID N++C DSL
Sbjct: 28  RVSTSLKIMNSKNHTNPRKCECFDLYDQLIPYKKAWSWQKSILNEKKALIDKNQECSDSL 87

Query: 102 IVLQHHPVYTMGTNSTEEYLNFSPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHK 161
           I+LQH  VYTMGT S+E YLNF  K++PF +YRTERGGEVTYHGPGQLVMYPIINLRNHK
Sbjct: 88  IILQHPSVYTMGTGSSENYLNFDIKNAPFDVYRTERGGEVTYHGPGQLVMYPIINLRNHK 147

Query: 162 MDLHWYLRALEEVVIRVLSSTFSINAHRVDGLTGVWSGNQKLAAIGIRVSKWITFHGLAL 221
           MDLHWYLR LEEVVIRVLSS F+INA R+DG TGVW GN+K+AAIGIRVSKW+T+HGLAL
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSKWMTYHGLAL 207

Query: 222 NVTTDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEFQNNIHLDNDCQLIDITFEALIQ 281
           NVTTDLTPFN IVPCGIRNR VG++K L+ + + + + +       D QL+DI  E+L++
Sbjct: 208 NVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHYNKLE-------DLQLLDIAHESLLK 267

Query: 282 EFSEVFQLDIHQKTIPTLE 297
           EFSEVFQL + ++T+  LE
Sbjct: 268 EFSEVFQLQMEKQTVFKLE 279

BLAST of Lag0040374 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 126.3 bits (316), Expect = 4.2e-29
Identity = 68/185 (36.76%), Postives = 105/185 (56.76%), Query Frame = 0

Query: 80  VREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFSPKDSP---FSIYRTERGGE 139
           ++EK+         PD+L+ LQH P YT+G   T+  L     +       ++ T+RGG+
Sbjct: 23  LQEKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIGAELHYTQRGGD 82

Query: 140 VTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHRVDGL-TGVWSG 199
           +T+HGP Q ++YPII+LR+       Y+  LE  +I   +S + + A   +   TGVW G
Sbjct: 83  ITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIE-FASIYGVKARAGNKCETGVWVG 142

Query: 200 NQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEF 259
           ++K+ AIG+R+S  IT HGLALN+  D+  F  IVPCGI ++ V +++   RE D     
Sbjct: 143 DRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLR---RETDTLLPS 202

Query: 260 QNNIH 261
           +  IH
Sbjct: 203 EEVIH 203

BLAST of Lag0040374 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 126.3 bits (316), Expect = 4.2e-29
Identity = 68/185 (36.76%), Postives = 105/185 (56.76%), Query Frame = 0

Query: 80  VREKIALIDANEDCPDSLIVLQHHPVYTMGTNSTEEYLNFSPKDSP---FSIYRTERGGE 139
           ++EK+         PD+L+ LQH P YT+G   T+  L     +       ++ T+RGG+
Sbjct: 23  LQEKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIGAELHYTQRGGD 82

Query: 140 VTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHRVDGL-TGVWSG 199
           +T+HGP Q ++YPII+LR+       Y+  LE  +I   +S + + A   +   TGVW G
Sbjct: 83  ITFHGPHQAILYPIISLRSIGFGARNYVETLERSMIE-FASIYGVKARAGNKCETGVWVG 142

Query: 200 NQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRHVGNIKELLREADLHTEF 259
           ++K+ AIG+R+S  IT HGLALN+  D+  F  IVPCGI ++ V +++   RE D     
Sbjct: 143 DRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLR---RETDTLLPS 202

Query: 260 QNNIH 261
           +  IH
Sbjct: 203 EEVIH 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150592.18.8e-14683.61plastidial lipoyltransferase 2-like [Momordica charantia] >XP_022150593.1 plasti... [more]
KAA0034631.12.4e-14381.29plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa] >TYK09182.1 plast... [more]
XP_016900264.12.4e-14381.29PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo][more]
XP_004135053.29.2e-14380.13octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158.... [more]
XP_031742006.11.2e-14281.43octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q948J96.5e-9969.05Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2... [more]
P0C7R21.6e-9765.64Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP... [more]
Q3M6981.6e-5252.69Octanoyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=2... [more]
Q8YSA42.7e-5245.85Octanoyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=1... [more]
Q8DKM73.8e-5145.22Octanoyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1D9U74.3e-14683.61Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3... [more]
A0A5D3CD131.2e-14381.29Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S4DWA51.2e-14381.29Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1[more]
A0A0A0KRC74.4e-14380.13Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=... [more]
A0A6J1G1547.6e-14382.52Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G31050.14.6e-10069.05Biotin/lipoate A/B protein ligase family [more]
AT1G47578.11.1e-9865.64Biotin/lipoate A/B protein ligase family [more]
AT1G04640.14.2e-2936.76lipoyltransferase 2 [more]
AT1G04640.24.2e-2936.76lipoyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 112..222
e-value: 1.0E-8
score: 35.1
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPROSITEPS51733BPL_LPL_CATALYTICcoord: 91..287
score: 56.484875
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 52..262
e-value: 1.4E-68
score: 232.2
NoneNo IPR availablePIRSRPIRSR016262-2PIRSR016262-2coord: 63..259
e-value: 3.2E-62
score: 207.4
NoneNo IPR availablePIRSRPIRSR016262-3PIRSR016262-3coord: 63..259
e-value: 3.2E-62
score: 207.4
NoneNo IPR availablePIRSRPIRSR016262-1PIRSR016262-1coord: 63..259
e-value: 3.2E-62
score: 207.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR10993:SF14BNAC03G77390D PROTEINcoord: 50..302
NoneNo IPR availablePANTHERPTHR10993OCTANOYLTRANSFERASEcoord: 50..302
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 56..248
IPR000544OctanoyltransferaseTIGRFAMTIGR00214TIGR00214coord: 88..254
e-value: 1.8E-56
score: 188.6
IPR000544OctanoyltransferaseHAMAPMF_00013LipBcoord: 56..286
score: 34.39846
IPR000544OctanoyltransferaseCDDcd16444LipBcoord: 63..283
e-value: 2.93233E-96
score: 280.917
IPR020605Octanoyltransferase, conserved sitePROSITEPS01313LIPBcoord: 133..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040374.1Lag0040374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009249 protein lipoylation
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016874 ligase activity
molecular_function GO:0033819 lipoyl(octanoyl) transferase activity
molecular_function GO:0102555 octanoyl transferase activity (acting on glycine-cleavage complex H protein)