Lag0040357 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040357
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein PIN-LIKES 2-like
Locationchr13: 4145912 .. 4147285 (-)
RNA-Seq ExpressionLag0040357
SyntenyLag0040357
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACATTAGGTCTAATGAAGAAGACATACTTGCTGCAATTGTGCCGTTAATGAAACTTCTCTGCCTCACAGTCATTGGATTAGTTCTTGCCCACCCAAAGACAAAAATCATCCCAAAAGAGACATTTAGGCTGCTTTGCAAACTTGTTTTCGTCTTGTTCTTGCCCTGCCTGATTTTTACTGAACTGGGCCAAAGTATTACGCTCGAGAGTTTCGCTCAATGGTGGTTCATCCCAGTAAATGTGCTACTCAGCACGGCCATCGGTTGTGCCCTTGGGTATTTAGTGGTTGCTAAATGTCATCCGCCCCCTCAGTTAAATAGAATGACTGTTATTGTGACAGCATTTGGTAATACTGGAAATCTCCCTCTTGCAGTTGTGGGGTCAGTTTGTCACACTTCAAACAACCCTTTTGGACCAAATTGTCACTCTAGTGGGGTGGCCTATGTCTCACTTGCTCAATGGATTTCTGTGATTCTAGCCTATACCTTCGTTTATCACATGATGGAACCTTCACCGGAATGTAGTGAGGTTGAAGATGAAGGCATTAAAATTGAAGAAGAACGAGCTGATGGTGATATTGGGAGGCCTCTCCTTGTAGAAGCTGAATGGCCTGGCGTTGAGGACAAAGAAACAGAACATTCTAAAACAGCCTTGATTGCTAGAATTTTCAGTAGCACTGAAATTCAATCGCAGAGCACTTCTACTGATATAAAACTCTCAGTAGGCGACAATGCTGACAGTCCCGAGTCTACTGATATCGAACTCTCAGGAGGTGACAGCGCTGACAGTCCCAAATTCACCAAATGCTTGGCTGAACCCAGAGTAGTGAGGAAGATGAGGATTGTTGCTGAGCAAACTCCAATACAGCACATCCTTCAACCCCCAACAATTGCTTCTCTAGTAACCATTATAATTGGGCTGATTCCACAACTGAAATCACTTTTCTTCGACTATGATGCCCCATTATCATTTGTCACAGACAGCCTAGAAATTTTAGCCCGTGCTACAGTGCCTTCTGTTATGCTTATTCTTGGGGGTATGCTCGCAGAGTACCCAAACGCATCTATATTAGGCCATCGAACCACGATCGGTATAATCATAGCGAGGCTCTTGGTTCTTCCTCTGCTGGGAATCGGGATAGTCACATTGGCTGATAAGCTGAATTTTCTTGTCTCTGGGGATCCAATGTACAGGTTTGTGCTTCTGTTGCAGTACACTACCCCAAGTGCTATTTTATTGGGAGCCATTGCCAGCTTGAGAGGATATGCAGTTAGGGAAGTTTCTGCACTTCTCTTCTGGCAACATATATTTGCTCTCTTCTCCCTTTCTCTATATGTTGTTATATACTTCAAGATAGTTGCATACGTTTGA

mRNA sequence

ATGGACATTAGGTCTAATGAAGAAGACATACTTGCTGCAATTGTGCCGTTAATGAAACTTCTCTGCCTCACAGTCATTGGATTAGTTCTTGCCCACCCAAAGACAAAAATCATCCCAAAAGAGACATTTAGGCTGCTTTGCAAACTTGTTTTCGTCTTGTTCTTGCCCTGCCTGATTTTTACTGAACTGGGCCAAAGTATTACGCTCGAGAGTTTCGCTCAATGGTGGTTCATCCCAGTAAATGTGCTACTCAGCACGGCCATCGGTTGTGCCCTTGGGTATTTAGTGGTTGCTAAATGTCATCCGCCCCCTCAGTTAAATAGAATGACTGTTATTGTGACAGCATTTGGTAATACTGGAAATCTCCCTCTTGCAGTTGTGGGGTCAGTTTGTCACACTTCAAACAACCCTTTTGGACCAAATTGTCACTCTAGTGGGGTGGCCTATGTCTCACTTGCTCAATGGATTTCTGTGATTCTAGCCTATACCTTCGTTTATCACATGATGGAACCTTCACCGGAATGTAGTGAGGTTGAAGATGAAGGCATTAAAATTGAAGAAGAACGAGCTGATGGTGATATTGGGAGGCCTCTCCTTGTAGAAGCTGAATGGCCTGGCGTTGAGGACAAAGAAACAGAACATTCTAAAACAGCCTTGATTGCTAGAATTTTCAGTAGCACTGAAATTCAATCGCAGAGCACTTCTACTGATATAAAACTCTCAGTAGGCGACAATGCTGACAGTCCCGAGTCTACTGATATCGAACTCTCAGGAGGTGACAGCGCTGACAGTCCCAAATTCACCAAATGCTTGGCTGAACCCAGAGTAGTGAGGAAGATGAGGATTGTTGCTGAGCAAACTCCAATACAGCACATCCTTCAACCCCCAACAATTGCTTCTCTAGTAACCATTATAATTGGGCTGATTCCACAACTGAAATCACTTTTCTTCGACTATGATGCCCCATTATCATTTGTCACAGACAGCCTAGAAATTTTAGCCCGTGCTACAGTGCCTTCTGTTATGCTTATTCTTGGGGGTATGCTCGCAGAGTACCCAAACGCATCTATATTAGGCCATCGAACCACGATCGGTATAATCATAGCGAGGCTCTTGGTTCTTCCTCTGCTGGGAATCGGGATAGTCACATTGGCTGATAAGCTGAATTTTCTTGTCTCTGGGGATCCAATGTACAGGTTTGTGCTTCTGTTGCAGTACACTACCCCAAGTGCTATTTTATTGGGAGCCATTGCCAGCTTGAGAGGATATGCAGTTAGGGAAGTTTCTGCACTTCTCTTCTGGCAACATATATTTGCTCTCTTCTCCCTTTCTCTATATGTTGTTATATACTTCAAGATAGTTGCATACGTTTGA

Coding sequence (CDS)

ATGGACATTAGGTCTAATGAAGAAGACATACTTGCTGCAATTGTGCCGTTAATGAAACTTCTCTGCCTCACAGTCATTGGATTAGTTCTTGCCCACCCAAAGACAAAAATCATCCCAAAAGAGACATTTAGGCTGCTTTGCAAACTTGTTTTCGTCTTGTTCTTGCCCTGCCTGATTTTTACTGAACTGGGCCAAAGTATTACGCTCGAGAGTTTCGCTCAATGGTGGTTCATCCCAGTAAATGTGCTACTCAGCACGGCCATCGGTTGTGCCCTTGGGTATTTAGTGGTTGCTAAATGTCATCCGCCCCCTCAGTTAAATAGAATGACTGTTATTGTGACAGCATTTGGTAATACTGGAAATCTCCCTCTTGCAGTTGTGGGGTCAGTTTGTCACACTTCAAACAACCCTTTTGGACCAAATTGTCACTCTAGTGGGGTGGCCTATGTCTCACTTGCTCAATGGATTTCTGTGATTCTAGCCTATACCTTCGTTTATCACATGATGGAACCTTCACCGGAATGTAGTGAGGTTGAAGATGAAGGCATTAAAATTGAAGAAGAACGAGCTGATGGTGATATTGGGAGGCCTCTCCTTGTAGAAGCTGAATGGCCTGGCGTTGAGGACAAAGAAACAGAACATTCTAAAACAGCCTTGATTGCTAGAATTTTCAGTAGCACTGAAATTCAATCGCAGAGCACTTCTACTGATATAAAACTCTCAGTAGGCGACAATGCTGACAGTCCCGAGTCTACTGATATCGAACTCTCAGGAGGTGACAGCGCTGACAGTCCCAAATTCACCAAATGCTTGGCTGAACCCAGAGTAGTGAGGAAGATGAGGATTGTTGCTGAGCAAACTCCAATACAGCACATCCTTCAACCCCCAACAATTGCTTCTCTAGTAACCATTATAATTGGGCTGATTCCACAACTGAAATCACTTTTCTTCGACTATGATGCCCCATTATCATTTGTCACAGACAGCCTAGAAATTTTAGCCCGTGCTACAGTGCCTTCTGTTATGCTTATTCTTGGGGGTATGCTCGCAGAGTACCCAAACGCATCTATATTAGGCCATCGAACCACGATCGGTATAATCATAGCGAGGCTCTTGGTTCTTCCTCTGCTGGGAATCGGGATAGTCACATTGGCTGATAAGCTGAATTTTCTTGTCTCTGGGGATCCAATGTACAGGTTTGTGCTTCTGTTGCAGTACACTACCCCAAGTGCTATTTTATTGGGAGCCATTGCCAGCTTGAGAGGATATGCAGTTAGGGAAGTTTCTGCACTTCTCTTCTGGCAACATATATTTGCTCTCTTCTCCCTTTCTCTATATGTTGTTATATACTTCAAGATAGTTGCATACGTTTGA

Protein sequence

MDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV
Homology
BLAST of Lag0040357 vs. NCBI nr
Match: XP_022150493.1 (protein PIN-LIKES 2-like [Momordica charantia])

HSP 1 Score: 697.6 bits (1799), Expect = 7.2e-197
Identity = 364/455 (80.00%), Postives = 398/455 (87.47%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           ++S+EED LAAIVPL+KLLCLTVIGLVLAHPKTK+IP+ TF+LL KLVF LFLPCLIFTE
Sbjct: 13  VKSSEEDALAAIVPLLKLLCLTVIGLVLAHPKTKVIPRATFKLLSKLVFALFLPCLIFTE 72

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LG+SITLE+ +QWWFIP+NVLLST IGC LGYLVV  CHPPPQLNR+TVI+TAFGNTGNL
Sbjct: 73  LGRSITLENLSQWWFIPINVLLSTGIGCVLGYLVVIICHPPPQLNRLTVIMTAFGNTGNL 132

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
           PLA+VGSVCHT +NPFG +CHSSGVAYVSLAQW+SVILAYTFVYHMMEP  +C E+ DE 
Sbjct: 133 PLAIVGSVCHTGDNPFGQHCHSSGVAYVSLAQWVSVILAYTFVYHMMEPPLDCCEIVDER 192

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSV 242
           I++EEE+AD D  RPLLVEAEWPG+EDKETEHSKT  IARIF+S    SQST        
Sbjct: 193 IEMEEEQADNDFSRPLLVEAEWPGIEDKETEHSKTPTIARIFNSISSTSQST-------- 252

Query: 243 GDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLV 302
                   STDIELSGG S DSPK   CLA+P VVR+M+IVAEQTPIQHI QPPTIASLV
Sbjct: 253 --------STDIELSGGCSTDSPKSINCLAQPDVVRRMKIVAEQTPIQHIFQPPTIASLV 312

Query: 303 TIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRT 362
           TIIIGLIP+LKSLFFDYDAPLSFV DSLEILARATVPSVML+LGGMLAE PN S L H+T
Sbjct: 313 TIIIGLIPRLKSLFFDYDAPLSFVKDSLEILARATVPSVMLVLGGMLAEGPNISKLSHQT 372

Query: 363 TIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRG 422
           TI IIIARLLVLPL GIGIVTLADKLNFLVSGDPM+RFVLLLQYTTPSAILLGAIASLRG
Sbjct: 373 TISIIIARLLVLPLFGIGIVTLADKLNFLVSGDPMFRFVLLLQYTTPSAILLGAIASLRG 432

Query: 423 YAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           YAVRE SALLFWQHIFALFSLS+Y++IYFKIV YV
Sbjct: 433 YAVREASALLFWQHIFALFSLSVYIIIYFKIVVYV 451

BLAST of Lag0040357 vs. NCBI nr
Match: XP_023541300.1 (protein PIN-LIKES 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.3 bits (1793), Expect = 3.6e-196
Identity = 363/456 (79.61%), Postives = 402/456 (88.16%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 12  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 71

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGC LGYLVVA CHPPP+ NR+TV++TAFGNTGNL
Sbjct: 72  LGQSITLESVTQWWFIPVNVLLSTAIGCVLGYLVVALCHPPPEFNRLTVVMTAFGNTGNL 131

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ V DEG
Sbjct: 132 TLAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVVLDEG 191

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+IEEERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S  I  Q+TSTDI++S 
Sbjct: 192 IEIEEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISITPQTTSTDIEISG 251

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LA+PRVVR++  +AEQTPIQHILQPPTIA L
Sbjct: 252 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAKPRVVRRLSHLAEQTPIQHILQPPTIAFL 311

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 312 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 371

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGIIIARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 372 TTIGIIIARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 431

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 432 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Lag0040357 vs. NCBI nr
Match: XP_022945293.1 (protein PIN-LIKES 2-like isoform X1 [Cucurbita moschata] >XP_022945294.1 protein PIN-LIKES 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 692.6 bits (1786), Expect = 2.3e-195
Identity = 362/456 (79.39%), Postives = 400/456 (87.72%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 12  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 71

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++TAFGNTGNL
Sbjct: 72  LGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVMTAFGNTGNL 131

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ + DEG
Sbjct: 132 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVILDEG 191

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S     Q+TSTDI++S 
Sbjct: 192 IEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQTTSTDIEISG 251

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LAEPRVVR++  VAEQTPIQHILQPPTIA L
Sbjct: 252 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHVAEQTPIQHILQPPTIAFL 311

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 312 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 371

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 372 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 431

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 432 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Lag0040357 vs. NCBI nr
Match: KAG6573917.1 (Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 691.8 bits (1784), Expect = 4.0e-195
Identity = 362/456 (79.39%), Postives = 400/456 (87.72%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 12  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 71

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++TAFGNTGNL
Sbjct: 72  LGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVMTAFGNTGNL 131

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ V DEG
Sbjct: 132 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVVLDEG 191

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S     Q+TSTDI++S 
Sbjct: 192 IEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQTTSTDIEISG 251

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LAEPRVVR++  +AEQTPIQHILQPPTIA L
Sbjct: 252 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHLAEQTPIQHILQPPTIAFL 311

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 312 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 371

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 372 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 431

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 432 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Lag0040357 vs. NCBI nr
Match: XP_022968440.1 (protein PIN-LIKES 2-like isoform X1 [Cucurbita maxima] >XP_022968441.1 protein PIN-LIKES 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 688.3 bits (1775), Expect = 4.4e-194
Identity = 360/456 (78.95%), Postives = 400/456 (87.72%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 17  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 76

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++TAFGNTGNL
Sbjct: 77  LGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVMTAFGNTGNL 136

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ V DEG
Sbjct: 137 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVVLDEG 196

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S  I  Q+TSTDI+ S 
Sbjct: 197 IEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQTTSTDIESSG 256

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LA PRV+R++  +AEQTPIQHILQPPTI+ L
Sbjct: 257 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHILQPPTISFL 316

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 317 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 376

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 377 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 436

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 437 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 472

BLAST of Lag0040357 vs. ExPASy Swiss-Prot
Match: Q9C999 (Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.4e-163
Identity = 301/454 (66.30%), Postives = 365/454 (80.40%), Query Frame = 0

Query: 2   DIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFT 61
           ++ S   DIL+ +VPL+KL+CLTVIGL+LAHPKT+++P+ TFRLL KLVF LFLPCLIFT
Sbjct: 8   NVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFT 67

Query: 62  ELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGN 121
           ELG+SITL++  QWWFIPVNVLLS  +G  +GYLVV  C PPP+ NR T+++TAFGNTGN
Sbjct: 68  ELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGN 127

Query: 122 LPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSE-VED 181
           L LA+V SVCHT  NPFGPNC+S GV+YVS AQW++VIL YT VYHMMEP  E  E VE+
Sbjct: 128 LLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEE 187

Query: 182 EGIKIEEERADG-DIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIK 241
           EG++IEE   +  D  RPLLVEAEWPG+EDKETEH KT  IAR+F+S    SQ++  ++ 
Sbjct: 188 EGVEIEEINVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 247

Query: 242 LSVGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIA 301
           L  G+             GG+S+ SP+  +CLAEPRV+R++R+VAEQTP++HILQPPTIA
Sbjct: 248 LG-GE------------YGGESS-SPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 307

Query: 302 SLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILG 361
           SL+ IIIG +PQLKS+ F YDAPLSF+TDSL I+  A VPSVML+LGGML+E PN S LG
Sbjct: 308 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNESTLG 367

Query: 362 HRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIAS 421
            RTTIGI +ARLLVLPL+GIGIV  ADKL  + S DPM++FVLLLQY+TPSAILLGAIAS
Sbjct: 368 LRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIAS 427

Query: 422 LRGYAVREVSALLFWQHIFALFSLSLYVVIYFKI 454
           LRGYAVRE SALLFWQHIFAL SL+ Y+VI+FK+
Sbjct: 428 LRGYAVREASALLFWQHIFALLSLTFYIVIFFKL 447

BLAST of Lag0040357 vs. ExPASy Swiss-Prot
Match: Q9LZN2 (Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.0e-73
Identity = 168/447 (37.58%), Postives = 255/447 (57.05%), Query Frame = 0

Query: 10  ILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITL 69
           I  A++P+ K+  +  +GL++A     I+P    +LL  LVF L LPCLIF++LGQ++TL
Sbjct: 28  IKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTL 87

Query: 70  ESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGS 129
           +   QWWFIPVNV+L T  G  +G++V +   PP    + T+I    GN GN+PL ++ +
Sbjct: 88  QKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAA 147

Query: 130 VCHTSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEE 189
           +C  ++NPFG +  C   G AY+S  QW+  I+ YT+VY M  P PE  + E+E + ++ 
Sbjct: 148 LCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEENLALKT 207

Query: 190 ERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNAD 249
              D    +  L+   +P                + FS T+      ST+          
Sbjct: 208 LPVDAAPEQVPLLTQNFP----------------KDFSPTQDLLPVQSTE---------- 267

Query: 250 SPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIG 309
            P    +   G                ++ +    + E+  ++ I+QP  +AS++ +I+G
Sbjct: 268 -PRGRGVSRKG----------------KIAQIFVFLYEKLKLKQIVQPAIVASILAMILG 327

Query: 310 LIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGII 369
            IP  K L F   APL F TDS  IL  A +P ++L LGG L   P +S LG +TT  II
Sbjct: 328 AIPFTKKLIFTNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPGSSKLGFKTTAAII 387

Query: 370 IARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVRE 429
           I RL+++P +G+GIVT+ADKL FL + D M+RFVLLLQ+T P+++L GA+A+LRG   RE
Sbjct: 388 IGRLVLVPPVGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RE 430

Query: 430 VSALLFWQHIFALFSLSLYVVIYFKIV 455
            +A+LFW HIFA+FS++ ++V+Y  I+
Sbjct: 448 SAAVLFWVHIFAIFSMAGWMVLYINIL 430

BLAST of Lag0040357 vs. ExPASy Swiss-Prot
Match: Q9SHL8 (Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.2e-46
Identity = 125/444 (28.15%), Postives = 220/444 (49.55%), Query Frame = 0

Query: 13  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 72
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 73  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 132
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 133 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERA 192
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 193 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPE 252
           + D+                E +H KT L+                              
Sbjct: 190 NSDL----------------EADH-KTHLL------------------------------ 249

Query: 253 STDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 312
                      A   K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 ----------GAPEDKENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 313 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIAR 372
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 373 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 432
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 433 LLFWQHIFALFSLSLYVVIYFKIV 455
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Lag0040357 vs. ExPASy Swiss-Prot
Match: Q9FKY4 (Protein PIN-LIKES 7 OS=Arabidopsis thaliana OX=3702 GN=PILS7 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.5e-45
Identity = 122/445 (27.42%), Postives = 221/445 (49.66%), Query Frame = 0

Query: 13  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 72
           A +P++++L ++V+G  LA     ++  +T R + KLVFV+F PC++F  L +++TL+  
Sbjct: 10  ASMPIVQVLLISVLGAFLATDYCSLLSADTRRSVNKLVFVVFTPCIMFANLAETVTLQDI 69

Query: 73  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 132
             WWF+P+NV ++  +G  LG+LVV   +P PQL+ + +   A GN GNL L +V ++C 
Sbjct: 70  ISWWFMPINVGITFLVGGILGWLVVKLLNPKPQLHGLIIATCASGNMGNLMLILVPAICD 129

Query: 133 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERA 192
              +PFG    C S G++Y S +  +     +T+ Y +         V     +     A
Sbjct: 130 EEGSPFGNRSVCRSIGLSYASFSMALGGFYIWTYSYQL---------VRSSATQFRALEA 189

Query: 193 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPE 252
            G +  P           +K+ +    AL+ +                           +
Sbjct: 190 AGLVKSP-----------NKDIDSDPHALLLK-------------------------PHQ 249

Query: 253 STDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 312
           + D+E+ G     +  + K L                 ++ +  PPTI +++  + G   
Sbjct: 250 NQDLEIQGKQKVSTRTYIKDLL-------------HQILEELFAPPTIGAILGFVFGATN 309

Query: 313 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIAR 372
            L++L    +APL  + DS+++L   T+P + LILGG L +   +S +     +G+II R
Sbjct: 310 WLRNLIIGENAPLRVIQDSVKLLGEGTIPCITLILGGNLIQGLRSSAVKKSVIVGVIIVR 369

Query: 373 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 432
            ++LP++G+G+V LA  L +L   DP++R+VL+LQ+  P A+ +  +A L   A  E S 
Sbjct: 370 YILLPVVGVGVVQLAGNLGYL-PPDPLFRYVLMLQFALPPAMNISTMAQLFDVAQDECSV 395

Query: 433 LLFWQHIFALFSLSLYVVIYFKIVA 456
           +  W ++ A  +L+++  I+  I++
Sbjct: 430 IFLWTYLVASLALTVWSTIFLSILS 395

BLAST of Lag0040357 vs. ExPASy Swiss-Prot
Match: F4HWB6 (Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 5.1e-44
Identity = 133/447 (29.75%), Postives = 217/447 (48.55%), Query Frame = 0

Query: 11  LAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLE 70
           + + +P+ K+L +T IG  LA  +  I+  +  + L  +VF +F P L+ + L ++IT E
Sbjct: 96  ITSSIPVAKILLITGIGFYLALDQVNILNHDARKQLNNIVFYVFSPSLVASSLSETITYE 155

Query: 71  SFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSV 130
           S  + WF+P+NVLL+  IG  LG++V+    PP  L  + V   A GN GN+PL ++ ++
Sbjct: 156 SMVKMWFMPLNVLLTFIIGSFLGWIVIKITKPPSHLRGIIVGCCAAGNLGNMPLIIIPAI 215

Query: 131 CHTSNNPFG--PNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEE 190
           C+   +PFG   +C   G+ Y++L+  I  I  +T+VY++M                   
Sbjct: 216 CNEKGSPFGDPESCEKFGLGYIALSMAIGAIYIWTYVYNLMR------------------ 275

Query: 191 RADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADS 250
                                         ++A     T I S S++  +         S
Sbjct: 276 ------------------------------MLANPAGETAINSTSSTMPL--------IS 335

Query: 251 PESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGL 310
           P+    E  G                +V +++  VAE+  ++ I  P TIA+L+ + +GL
Sbjct: 336 PKVEVAEQVG-------------TWGKVKQRVCSVAEKINLRTIFAPSTIAALIALAVGL 395

Query: 311 IPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIII 370
            P L+ L     APL  + DS+ +L    +P + LI+GG L      S +     +G+++
Sbjct: 396 NPLLRKLLVGNTAPLRVIEDSVSLLGDGAIPVLTLIVGGNLLNGLRGSGINKSVIMGVVV 455

Query: 371 ARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREV 430
            R L+LP+LG+ IV  A  L  LV+ +P+Y+FVLLLQY  P A+ LG I  L G    E 
Sbjct: 456 VRYLLLPILGVFIVRGAHYLG-LVTSEPLYQFVLLLQYVVPPAMNLGTITQLFGSGESEC 472

Query: 431 SALLFWQHIFALFSLSLYVVIYFKIVA 456
           S +LFW +  A  SL+++   +  +VA
Sbjct: 516 SVILFWSYALASVSLTVWPTFFMWLVA 472

BLAST of Lag0040357 vs. ExPASy TrEMBL
Match: A0A6J1D9L3 (protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111018630 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.5e-197
Identity = 364/455 (80.00%), Postives = 398/455 (87.47%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           ++S+EED LAAIVPL+KLLCLTVIGLVLAHPKTK+IP+ TF+LL KLVF LFLPCLIFTE
Sbjct: 13  VKSSEEDALAAIVPLLKLLCLTVIGLVLAHPKTKVIPRATFKLLSKLVFALFLPCLIFTE 72

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LG+SITLE+ +QWWFIP+NVLLST IGC LGYLVV  CHPPPQLNR+TVI+TAFGNTGNL
Sbjct: 73  LGRSITLENLSQWWFIPINVLLSTGIGCVLGYLVVIICHPPPQLNRLTVIMTAFGNTGNL 132

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
           PLA+VGSVCHT +NPFG +CHSSGVAYVSLAQW+SVILAYTFVYHMMEP  +C E+ DE 
Sbjct: 133 PLAIVGSVCHTGDNPFGQHCHSSGVAYVSLAQWVSVILAYTFVYHMMEPPLDCCEIVDER 192

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSV 242
           I++EEE+AD D  RPLLVEAEWPG+EDKETEHSKT  IARIF+S    SQST        
Sbjct: 193 IEMEEEQADNDFSRPLLVEAEWPGIEDKETEHSKTPTIARIFNSISSTSQST-------- 252

Query: 243 GDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLV 302
                   STDIELSGG S DSPK   CLA+P VVR+M+IVAEQTPIQHI QPPTIASLV
Sbjct: 253 --------STDIELSGGCSTDSPKSINCLAQPDVVRRMKIVAEQTPIQHIFQPPTIASLV 312

Query: 303 TIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRT 362
           TIIIGLIP+LKSLFFDYDAPLSFV DSLEILARATVPSVML+LGGMLAE PN S L H+T
Sbjct: 313 TIIIGLIPRLKSLFFDYDAPLSFVKDSLEILARATVPSVMLVLGGMLAEGPNISKLSHQT 372

Query: 363 TIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRG 422
           TI IIIARLLVLPL GIGIVTLADKLNFLVSGDPM+RFVLLLQYTTPSAILLGAIASLRG
Sbjct: 373 TISIIIARLLVLPLFGIGIVTLADKLNFLVSGDPMFRFVLLLQYTTPSAILLGAIASLRG 432

Query: 423 YAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           YAVRE SALLFWQHIFALFSLS+Y++IYFKIV YV
Sbjct: 433 YAVREASALLFWQHIFALFSLSVYIIIYFKIVVYV 451

BLAST of Lag0040357 vs. ExPASy TrEMBL
Match: A0A6J1G0E7 (protein PIN-LIKES 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449580 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.1e-195
Identity = 362/456 (79.39%), Postives = 400/456 (87.72%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 12  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 71

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++TAFGNTGNL
Sbjct: 72  LGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVMTAFGNTGNL 131

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ + DEG
Sbjct: 132 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVILDEG 191

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S     Q+TSTDI++S 
Sbjct: 192 IEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQTTSTDIEISG 251

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LAEPRVVR++  VAEQTPIQHILQPPTIA L
Sbjct: 252 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHVAEQTPIQHILQPPTIAFL 311

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 312 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 371

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 372 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 431

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 432 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Lag0040357 vs. ExPASy TrEMBL
Match: A0A6J1HUV6 (protein PIN-LIKES 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467687 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.1e-194
Identity = 360/456 (78.95%), Postives = 400/456 (87.72%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 17  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 76

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++TAFGNTGNL
Sbjct: 77  LGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVMTAFGNTGNL 136

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ V DEG
Sbjct: 137 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVVLDEG 196

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S  I  Q+TSTDI+ S 
Sbjct: 197 IEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQTTSTDIESSG 256

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LA PRV+R++  +AEQTPIQHILQPPTI+ L
Sbjct: 257 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHILQPPTISFL 316

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 317 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 376

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 377 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 436

Query: 423 GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
           GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 437 GYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 472

BLAST of Lag0040357 vs. ExPASy TrEMBL
Match: A0A6J1HTI4 (protein PIN-LIKES 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467687 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.1e-179
Identity = 337/444 (75.90%), Postives = 378/444 (85.14%), Query Frame = 0

Query: 3   IRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTE 62
           I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF LFLPCLIFTE
Sbjct: 17  IEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFALFLPCLIFTE 76

Query: 63  LGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNL 122
           LGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++TAFGNTGNL
Sbjct: 77  LGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVMTAFGNTGNL 136

Query: 123 PLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEG 182
            LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P PEC+ V DEG
Sbjct: 137 ALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPPECNVVLDEG 196

Query: 183 IKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS- 242
           I+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S  I  Q+TSTDI+ S 
Sbjct: 197 IEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQTTSTDIESSG 256

Query: 243 VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 302
            GD  D+P+  DIEL+G +  +SPK T+ LA PRV+R++  +AEQTPIQHILQPPTI+ L
Sbjct: 257 GGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHILQPPTISFL 316

Query: 303 VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 362
             IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE  N S LGHR
Sbjct: 317 AAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAECSNTSTLGHR 376

Query: 363 TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 422
           TTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAILLGA+ASL 
Sbjct: 377 TTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAILLGALASLG 436

Query: 423 GYAVREVSALLFWQHIFALFSLSL 446
           GYA+R + ALL    + +L  + L
Sbjct: 437 GYAIR-IHALLLMMSVESLMDIFL 459

BLAST of Lag0040357 vs. ExPASy TrEMBL
Match: A0A6A6MXI7 (ABC transporter domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_041171 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.2e-176
Identity = 328/456 (71.93%), Postives = 380/456 (83.33%), Query Frame = 0

Query: 2    DIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFT 61
            ++RS+ E+++ AIVPLMKLLCL +IGL+LAHPKT+IIP+ TF+LL KLVF LFLPCLIFT
Sbjct: 693  NMRSSGENLVTAIVPLMKLLCLIIIGLILAHPKTQIIPRATFKLLSKLVFALFLPCLIFT 752

Query: 62   ELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGN 121
            ELG+SITLE+FA+WWFIPVNVL+ST IG  LG LVVA CHPPPQ NR T+I+TAFGNTGN
Sbjct: 753  ELGESITLENFARWWFIPVNVLVSTIIGFFLGLLVVAICHPPPQYNRFTIIMTAFGNTGN 812

Query: 122  LPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDE 181
            LPLA+VGSVCHT ++PFG +CHSSGVAYVS AQW++VIL YT VYHMMEP  +  E+ +E
Sbjct: 813  LPLAIVGSVCHTKDSPFGAHCHSSGVAYVSFAQWVAVILVYTLVYHMMEPPLQYYEIVEE 872

Query: 182  GIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLS 241
              +IEE+R   D+ RPLL+EAEWPG+EDKETEHSKT  IARIF+S    SQ         
Sbjct: 873  EFEIEEQRPTPDVSRPLLIEAEWPGIEDKETEHSKTPFIARIFNSISSLSQI-------- 932

Query: 242  VGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASL 301
                       DIEL+G  +A+SP+  +CLAEPRVVR++RIVAEQTPI+HILQPPTIASL
Sbjct: 933  --------NFPDIELAGESTANSPRSIRCLAEPRVVRRIRIVAEQTPIRHILQPPTIASL 992

Query: 302  VTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHR 361
              IIIG++PQLK+  F YDAPLSF+TDSLEILA A VPSVMLILGGMLAE PN S LG  
Sbjct: 993  FAIIIGMVPQLKAFVFGYDAPLSFITDSLEILAGAMVPSVMLILGGMLAEGPNESTLGLP 1052

Query: 362  TTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLR 421
            TTIGI +ARLLVLPLLGIGIV LADKLNFLV GD MYRFVLLLQYTTPSAILLGAIASLR
Sbjct: 1053 TTIGISVARLLVLPLLGIGIVALADKLNFLVKGDAMYRFVLLLQYTTPSAILLGAIASLR 1112

Query: 422  GYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 458
            GYAV+E SALLFWQH+FALFSLSLY+VIYFK+++Y+
Sbjct: 1113 GYAVKEASALLFWQHVFALFSLSLYIVIYFKLLSYI 1132

BLAST of Lag0040357 vs. TAIR 10
Match: AT1G71090.1 (Auxin efflux carrier family protein )

HSP 1 Score: 576.6 bits (1485), Expect = 1.7e-164
Identity = 301/454 (66.30%), Postives = 365/454 (80.40%), Query Frame = 0

Query: 2   DIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFT 61
           ++ S   DIL+ +VPL+KL+CLTVIGL+LAHPKT+++P+ TFRLL KLVF LFLPCLIFT
Sbjct: 8   NVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFT 67

Query: 62  ELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGN 121
           ELG+SITL++  QWWFIPVNVLLS  +G  +GYLVV  C PPP+ NR T+++TAFGNTGN
Sbjct: 68  ELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGN 127

Query: 122 LPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSE-VED 181
           L LA+V SVCHT  NPFGPNC+S GV+YVS AQW++VIL YT VYHMMEP  E  E VE+
Sbjct: 128 LLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEE 187

Query: 182 EGIKIEEERADG-DIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIK 241
           EG++IEE   +  D  RPLLVEAEWPG+EDKETEH KT  IAR+F+S    SQ++  ++ 
Sbjct: 188 EGVEIEEINVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 247

Query: 242 LSVGDNADSPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIA 301
           L  G+             GG+S+ SP+  +CLAEPRV+R++R+VAEQTP++HILQPPTIA
Sbjct: 248 LG-GE------------YGGESS-SPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 307

Query: 302 SLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILG 361
           SL+ IIIG +PQLKS+ F YDAPLSF+TDSL I+  A VPSVML+LGGML+E PN S LG
Sbjct: 308 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNESTLG 367

Query: 362 HRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIAS 421
            RTTIGI +ARLLVLPL+GIGIV  ADKL  + S DPM++FVLLLQY+TPSAILLGAIAS
Sbjct: 368 LRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIAS 427

Query: 422 LRGYAVREVSALLFWQHIFALFSLSLYVVIYFKI 454
           LRGYAVRE SALLFWQHIFAL SL+ Y+VI+FK+
Sbjct: 428 LRGYAVREASALLFWQHIFALLSLTFYIVIFFKL 447

BLAST of Lag0040357 vs. TAIR 10
Match: AT5G01990.1 (Auxin efflux carrier family protein )

HSP 1 Score: 278.9 bits (712), Expect = 7.5e-75
Identity = 168/447 (37.58%), Postives = 255/447 (57.05%), Query Frame = 0

Query: 10  ILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITL 69
           I  A++P+ K+  +  +GL++A     I+P    +LL  LVF L LPCLIF++LGQ++TL
Sbjct: 28  IKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTL 87

Query: 70  ESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGS 129
           +   QWWFIPVNV+L T  G  +G++V +   PP    + T+I    GN GN+PL ++ +
Sbjct: 88  QKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAA 147

Query: 130 VCHTSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEE 189
           +C  ++NPFG +  C   G AY+S  QW+  I+ YT+VY M  P PE  + E+E + ++ 
Sbjct: 148 LCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEENLALKT 207

Query: 190 ERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNAD 249
              D    +  L+   +P                + FS T+      ST+          
Sbjct: 208 LPVDAAPEQVPLLTQNFP----------------KDFSPTQDLLPVQSTE---------- 267

Query: 250 SPESTDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIG 309
            P    +   G                ++ +    + E+  ++ I+QP  +AS++ +I+G
Sbjct: 268 -PRGRGVSRKG----------------KIAQIFVFLYEKLKLKQIVQPAIVASILAMILG 327

Query: 310 LIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGII 369
            IP  K L F   APL F TDS  IL  A +P ++L LGG L   P +S LG +TT  II
Sbjct: 328 AIPFTKKLIFTNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPGSSKLGFKTTAAII 387

Query: 370 IARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVRE 429
           I RL+++P +G+GIVT+ADKL FL + D M+RFVLLLQ+T P+++L GA+A+LRG   RE
Sbjct: 388 IGRLVLVPPVGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RE 430

Query: 430 VSALLFWQHIFALFSLSLYVVIYFKIV 455
            +A+LFW HIFA+FS++ ++V+Y  I+
Sbjct: 448 SAAVLFWVHIFAIFSMAGWMVLYINIL 430

BLAST of Lag0040357 vs. TAIR 10
Match: AT2G17500.1 (Auxin efflux carrier family protein )

HSP 1 Score: 187.2 bits (474), Expect = 3.0e-47
Identity = 125/444 (28.15%), Postives = 220/444 (49.55%), Query Frame = 0

Query: 13  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 72
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 73  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 132
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 133 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERA 192
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 193 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPE 252
           + D+                E +H KT L+                              
Sbjct: 190 NSDL----------------EADH-KTHLL------------------------------ 249

Query: 253 STDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 312
                      A   K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 ----------GAPEDKENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 313 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIAR 372
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 373 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 432
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 433 LLFWQHIFALFSLSLYVVIYFKIV 455
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Lag0040357 vs. TAIR 10
Match: AT2G17500.3 (Auxin efflux carrier family protein )

HSP 1 Score: 187.2 bits (474), Expect = 3.0e-47
Identity = 125/444 (28.15%), Postives = 220/444 (49.55%), Query Frame = 0

Query: 13  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 72
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 73  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 132
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 133 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERA 192
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 193 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPE 252
           + D+                E +H KT L+                              
Sbjct: 190 NSDL----------------EADH-KTHLL------------------------------ 249

Query: 253 STDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 312
                      A   K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 ----------GAPEDKENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 313 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIAR 372
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 373 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 432
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 433 LLFWQHIFALFSLSLYVVIYFKIV 455
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Lag0040357 vs. TAIR 10
Match: AT2G17500.2 (Auxin efflux carrier family protein )

HSP 1 Score: 187.2 bits (474), Expect = 3.0e-47
Identity = 125/444 (28.15%), Postives = 220/444 (49.55%), Query Frame = 0

Query: 13  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 72
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 73  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 132
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 133 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEVEDEGIKIEEERA 192
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 193 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTEIQSQSTSTDIKLSVGDNADSPE 252
           + D+                E +H KT L+                              
Sbjct: 190 NSDL----------------EADH-KTHLL------------------------------ 249

Query: 253 STDIELSGGDSADSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 312
                      A   K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 ----------GAPEDKENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 313 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNASILGHRTTIGIIIAR 372
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 373 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 432
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 433 LLFWQHIFALFSLSLYVVIYFKIV 455
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150493.17.2e-19780.00protein PIN-LIKES 2-like [Momordica charantia][more]
XP_023541300.13.6e-19679.61protein PIN-LIKES 2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022945293.12.3e-19579.39protein PIN-LIKES 2-like isoform X1 [Cucurbita moschata] >XP_022945294.1 protein... [more]
KAG6573917.14.0e-19579.39Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968440.14.4e-19478.95protein PIN-LIKES 2-like isoform X1 [Cucurbita maxima] >XP_022968441.1 protein P... [more]
Match NameE-valueIdentityDescription
Q9C9992.4e-16366.30Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1[more]
Q9LZN21.0e-7337.58Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1[more]
Q9SHL84.2e-4628.15Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1[more]
Q9FKY43.5e-4527.42Protein PIN-LIKES 7 OS=Arabidopsis thaliana OX=3702 GN=PILS7 PE=2 SV=1[more]
F4HWB65.1e-4429.75Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D9L33.5e-19780.00protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111018630 PE=4 SV=... [more]
A0A6J1G0E71.1e-19579.39protein PIN-LIKES 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144958... [more]
A0A6J1HUV62.1e-19478.95protein PIN-LIKES 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467687 ... [more]
A0A6J1HTI41.1e-17975.90protein PIN-LIKES 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467687 ... [more]
A0A6A6MXI71.2e-17671.93ABC transporter domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714... [more]
Match NameE-valueIdentityDescription
AT1G71090.11.7e-16466.30Auxin efflux carrier family protein [more]
AT5G01990.17.5e-7537.58Auxin efflux carrier family protein [more]
AT2G17500.13.0e-4728.15Auxin efflux carrier family protein [more]
AT2G17500.33.0e-4728.15Auxin efflux carrier family protein [more]
AT2G17500.23.0e-4728.15Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 14..447
e-value: 2.5E-84
score: 282.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 235..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 235..262
NoneNo IPR availablePANTHERPTHR31419:SF12AUXIN EFFLUX CARRIER FAMILY PROTEIN-RELATEDcoord: 2..454
IPR039305Protein PIN-LIKES 2/6PANTHERPTHR31419PROTEIN PIN-LIKES 2coord: 2..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040357.1Lag0040357.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0080162 intracellular auxin transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane