Lag0040275 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040275 vs. NCBI nr
Match: XP_022968284.1 (uncharacterized protein LOC111467561 [Cucurbita maxima] >XP_022968285.1 uncharacterized protein LOC111467561 [Cucurbita maxima] >XP_022968286.1 uncharacterized protein LOC111467561 [Cucurbita maxima])

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-190
Identity = 381/435 (87.59%), Postives = 405/435 (93.10%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLSARPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSARPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLEAYE STEMQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEAYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHMGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD GNGESSSSTLEE++SSFQ C+KMESESAEI TH+EES+NK +++++S
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEEKNSSFQSCKKMESESAEIITHIEESDNK-SSEHLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 434

BLAST of Lag0040275 vs. NCBI nr
Match: KAG6573979.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013036.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.2 bits (1733), Expect = 3.1e-189
Identity = 379/435 (87.13%), Postives = 405/435 (93.10%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGR+FKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRNFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLSARPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSARPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLEAYE STEMQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEAYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           K+KDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KFKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHRGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD GNGESSSSTLEE++SSFQ CEKMESESAEI T++EES+NK +++++S
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEEKNSSFQSCEKMESESAEIITNIEESDNK-SSEHLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 434

BLAST of Lag0040275 vs. NCBI nr
Match: XP_038893458.1 (uncharacterized protein LOC120082250 isoform X1 [Benincasa hispida])

HSP 1 Score: 670.6 bits (1729), Expect = 9.0e-189
Identity = 380/435 (87.36%), Postives = 405/435 (93.10%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVR+S ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRASHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVVLERIK LE  RECPEE+KEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKLLEHERECPEEIKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PEL+EIRSVITSRFGKEFTAR IELRNNCAV PMIVQKLSARP SLEIKLKLLKQIASE 
Sbjct: 121 PELIEIRSVITSRFGKEFTARAIELRNNCAVGPMIVQKLSARPSSLEIKLKLLKQIASEH 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           GITLKDLEAYEVSTEMQE+VVAEEKK++PQQETKVE VFQILPEEIEKD+RYADSMRGRK
Sbjct: 181 GITLKDLEAYEVSTEMQERVVAEEKKEQPQQETKVESVFQILPEEIEKDDRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD DDLS+  SKPRKS+ET ESKN +
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSSPSSKPRKSTETLESKNAD 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           +EL+S+NKETH GSTSIE EESE+  E KQP+SSSSSEAGDASLKENEIPIEAINII+LL
Sbjct: 301 MELKSENKETHGGSTSIEAEESESGIESKQPISSSSSEAGDASLKENEIPIEAINIIDLL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD GNGESSSSTLEER+S F+ C K+ESESAEIT + +ES+NKNTAQN+S
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEERNSCFE-CHKIESESAEITANNDESDNKNTAQNLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IG+EAV  TIDVRGH
Sbjct: 421 IGNEAVITTIDVRGH 434

BLAST of Lag0040275 vs. NCBI nr
Match: XP_022945818.1 (uncharacterized protein LOC111449950 [Cucurbita moschata])

HSP 1 Score: 666.8 bits (1719), Expect = 1.3e-187
Identity = 376/435 (86.44%), Postives = 402/435 (92.41%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDA LGRSFKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDAFLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLS+RPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSSRPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLE YE STEMQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEGYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           K+KDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KFKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHRGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           E DL FDESDVD GNGESSSSTLEE++SSFQ CEKMESESAEI T++EES+NK +++++S
Sbjct: 361 ETDLAFDESDVDAGNGESSSSTLEEKNSSFQSCEKMESESAEIITNIEESDNK-SSEHLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 434

BLAST of Lag0040275 vs. NCBI nr
Match: XP_023541747.1 (uncharacterized protein LOC111801812 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.2 bits (1715), Expect = 3.8e-187
Identity = 377/435 (86.67%), Postives = 399/435 (91.72%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDA LGRSFKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDAFLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQK LDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKQLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLSARPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSARPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLEAYE ST+MQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEAYEDSTKMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHMGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD GNGESSSSTLEE++ SFQ CEKMESESAEI T++EES+NK++    S
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEEKNPSFQSCEKMESESAEIITNIEESDNKSSE---S 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 432

BLAST of Lag0040275 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-16
Identity = 56/177 (31.64%), Postives = 99/177 (55.93%), Query Frame = 0

Query: 10  GRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVIKE 69
           G S+ + + K  + LA+SR+ +L N++       + +V +LL+  ++E A  RVE +I++
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDFPELVEIRSV 129
           + L++ + IIE  CE++  RI  +    E P E+KE+I  L+++S R    PEL +I++ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 ITSRFGKEFTARVIELRNNC----AVSPMIVQKLSARPPSLEIKLKLLKQIASEIGI 183
           + +++GK      +E   NC     V+P IV KLS   P   I  + L +IA +  +
Sbjct: 125 LKAKYGKG-----LENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNV 175

BLAST of Lag0040275 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-13
Identity = 50/176 (28.41%), Postives = 92/176 (52.27%), Query Frame = 0

Query: 8   LLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRC-GDFPELVEI 127
           +E  L++A  I+E YC+++L R   ++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGI 183
              + +++ KE+  ++        V+  ++ KLS   P   +  + L +IA    +
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Lag0040275 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-13
Identity = 50/176 (28.41%), Postives = 92/176 (52.27%), Query Frame = 0

Query: 8   LLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRC-GDFPELVEI 127
           +E  L++A  I+E YC+++L R   ++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGI 183
              + +++ KE+  ++        V+  ++ KLS   P   +  + L +IA    +
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Lag0040275 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-13
Identity = 50/176 (28.41%), Postives = 92/176 (52.27%), Query Frame = 0

Query: 8   LLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVI 67
           +LG  FK  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRC-GDFPELVEI 127
           +E  L++A  I+E YC+++L R   ++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGI 183
              + +++ KE+  ++        V+  ++ KLS   P   +  + L +IA    +
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Lag0040275 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.5e-13
Identity = 49/176 (27.84%), Postives = 91/176 (51.70%), Query Frame = 0

Query: 8   LLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVI 67
           +LG   K  R +  + L I+RL +L  ++   + +AR ++   L  G  ERA  RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRC-GDFPELVEI 127
           +E  L++A  I+E YC+++L R   ++  +E    L E++S LI+A+ R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 RSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGI 183
              + +++ KE+  ++        V+  ++ KLS   P   +  + L +IA    +
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNV 175

BLAST of Lag0040275 vs. ExPASy TrEMBL
Match: A0A6J1HWT1 (uncharacterized protein LOC111467561 OS=Cucurbita maxima OX=3661 GN=LOC111467561 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.0e-190
Identity = 381/435 (87.59%), Postives = 405/435 (93.10%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLSARPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSARPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLEAYE STEMQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEAYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHMGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD GNGESSSSTLEE++SSFQ C+KMESESAEI TH+EES+NK +++++S
Sbjct: 361 EKDLAFDESDVDAGNGESSSSTLEEKNSSFQSCKKMESESAEIITHIEESDNK-SSEHLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 434

BLAST of Lag0040275 vs. ExPASy TrEMBL
Match: A0A6J1G222 (uncharacterized protein LOC111449950 OS=Cucurbita moschata OX=3662 GN=LOC111449950 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 6.3e-188
Identity = 376/435 (86.44%), Postives = 402/435 (92.41%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDA LGRSFKT RFKALVNLAISRLAVLANQRQVR + ARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDAFLGRSFKTTRFKALVNLAISRLAVLANQRQVRHTHARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RVEQVIKEQKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  LRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PELVEIRSVI SRFGK+FTA+ IELRNNCAVSPMIVQKLS+RPPSLEIK+KLLKQIASE 
Sbjct: 121 PELVEIRSVIASRFGKDFTAKAIELRNNCAVSPMIVQKLSSRPPSLEIKMKLLKQIASEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           G+TLKDLE YE STEMQEKVVAEE KD+P QETKVEGVFQILPEEIEKD+RYADSMRGR+
Sbjct: 181 GVTLKDLEGYEDSTEMQEKVVAEENKDQPAQETKVEGVFQILPEEIEKDSRYADSMRGRR 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           K+KDVADAAQAAFESAAYAAAAARAAVELSQSKSLD+DDL+ L +KPRKSSETDESKN E
Sbjct: 241 KFKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDQDDLNTLSTKPRKSSETDESKNIE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
           IELES+NK  H GSTSIEVEESE+  EL QPVSSSSSEAGD SLK+NEIPIEAINIIELL
Sbjct: 301 IELESENKVIHRGSTSIEVEESESPAELMQPVSSSSSEAGDVSLKDNEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           E DL FDESDVD GNGESSSSTLEE++SSFQ CEKMESESAEI T++EES+NK +++++S
Sbjct: 361 ETDLAFDESDVDAGNGESSSSTLEEKNSSFQSCEKMESESAEIITNIEESDNK-SSEHLS 420

Query: 421 IGDEAVERTIDVRGH 436
           IGDEAV RT DVRGH
Sbjct: 421 IGDEAVVRTTDVRGH 434

BLAST of Lag0040275 vs. ExPASy TrEMBL
Match: A0A0A0KUG8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608550 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.1e-187
Identity = 380/435 (87.36%), Postives = 398/435 (91.49%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHERECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PEL+EIRSVIT+RFGKEFTAR IELRNNC+V PMIVQKLSAR  SLEIKLKLLKQIASE 
Sbjct: 121 PELIEIRSVITTRFGKEFTARAIELRNNCSVGPMIVQKLSARSASLEIKLKLLKQIASEH 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           GITLKDLEAYE STE +  V+AEE K++PQQETK EGVFQILPEEIEKDNRYADSMRGRK
Sbjct: 181 GITLKDLEAYEASTEEKVMVMAEETKEQPQQETKEEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD DDLSNL  KPRKS+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
            E+   NKETH GS+SIEVEESENE ELKQPVSSSSSEAGDASLKENEIPIEAINIIELL
Sbjct: 301 KEM---NKETHGGSSSIEVEESENEIELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD  NGESS+S LEE++ SF  CEKMESESAEIT + EES+N +TAQN S
Sbjct: 361 EKDLTFDESDVDAANGESSNSALEEKNLSFVSCEKMESESAEITANNEESDNNDTAQNTS 420

Query: 421 IGDEAVERTIDVRGH 436
           IG+EAV RTIDVRGH
Sbjct: 421 IGNEAVIRTIDVRGH 432

BLAST of Lag0040275 vs. ExPASy TrEMBL
Match: A0A5A7STU8 (Midasin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00860 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.1e-183
Identity = 380/435 (87.36%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PEL+EIRSVIT+RFGKEFTAR IELRNNCAV PMIVQKLSARP SLEIKLKLLKQIASE 
Sbjct: 121 PELIEIRSVITNRFGKEFTARAIELRNNCAVGPMIVQKLSARPASLEIKLKLLKQIASEH 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           GITLKDLE YEVS+E +  V+AEEKK+ PQQETKVEGVFQILPEEIEKDNRYADSMRGRK
Sbjct: 181 GITLKDLEDYEVSSEEKVVVMAEEKKEEPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD DDLSNL  KPRKS+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
            E+   NKE  SGSTSIE EESEN TELKQPVSSSSSEAGDASLKENEIPIEAINII LL
Sbjct: 301 KEM---NKE-ESGSTSIE-EESENGTELKQPVSSSSSEAGDASLKENEIPIEAINIIGLL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD   GESS S +EER+ SF+  EKMESESAEITT+ EES+N +TAQN+S
Sbjct: 361 EKDLAFDESDVDAAKGESSGSAIEERNLSFESREKMESESAEITTNNEESDNDDTAQNIS 420

Query: 421 IGDEAVERTIDVRGH 436
           IG+EAV RTIDVRGH
Sbjct: 421 IGNEAVIRTIDVRGH 430

BLAST of Lag0040275 vs. ExPASy TrEMBL
Match: A0A1S3BEW7 (midasin OS=Cucumis melo OX=3656 GN=LOC103489273 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.1e-183
Identity = 380/435 (87.36%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKT RFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGH ERAL
Sbjct: 1   MGKKLDALLGRSFKTTRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHHERAL 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
           FRVEQVIK+QKLLDAYAIIESYCEVVLERIKQLE  RECPEELKEAISGLIFASSRCGDF
Sbjct: 61  FRVEQVIKDQKLLDAYAIIESYCEVVLERIKQLEHQRECPEELKEAISGLIFASSRCGDF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PEL+EIRSVIT+RFGKEFTAR IELRNNCAV PMIVQKLSARP SLEIKLKLLKQIASE 
Sbjct: 121 PELIEIRSVITNRFGKEFTARAIELRNNCAVGPMIVQKLSARPASLEIKLKLLKQIASEH 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240
           GITLKDLE YEVS+E +  V+AEEKK+ PQQETKVEGVFQILPEEIEKDNRYADSMRGRK
Sbjct: 181 GITLKDLEDYEVSSEEKVVVMAEEKKEEPQQETKVEGVFQILPEEIEKDNRYADSMRGRK 240

Query: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDESKNTE 300
           KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLD DDLSNL  KPRKS+ET ESKNTE
Sbjct: 241 KYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDHDDLSNLSPKPRKSTETLESKNTE 300

Query: 301 IELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEAINIIELL 360
            E+   NKE  SGSTSIE EESEN TELKQPVSSSSSEAGDASLKENEIPIEAINII LL
Sbjct: 301 KEM---NKE-ESGSTSIE-EESENGTELKQPVSSSSSEAGDASLKENEIPIEAINIIGLL 360

Query: 361 EKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEITTHVEESENKNTAQNVS 420
           EKDL FDESDVD   GESS S +EER+ SF+  EKMESESAEITT+ EES+N +TAQN+S
Sbjct: 361 EKDLAFDESDVDAAKGESSGSAIEERNLSFESREKMESESAEITTNNEESDNDDTAQNIS 420

Query: 421 IGDEAVERTIDVRGH 436
           IG+EAV RTIDVRGH
Sbjct: 421 IGNEAVIRTIDVRGH 430

BLAST of Lag0040275 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-65
Identity = 188/446 (42.15%), Postives = 259/446 (58.07%), Query Frame = 0

Query: 1   MGKKLDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERAL 60
           MGKKLDALLGRSFKTN+FK+L+ LA++RL++L NQRQ R SQA SDV +LL+LG  E A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  FRVEQVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDF 120
            RV+QV+K+Q  LD    I  Y  + L+RI   E  R+CPEEL EA+SGL+FA+SR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELVEIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEI 180
           PEL EIR+V+ SRFGK+  AR IELR+NC V P I+QKLS RPP  E+++K LK+IA+E 
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GITLKDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEI-EKDNR-----YAD 240
            I LK L+    STE                 T ++G   +   ++  KD R      +D
Sbjct: 181 NIVLK-LDQASTSTE---------------GTTNMQGTSDVSKTKLTSKDGRGEGYGLSD 240

Query: 241 SM-RGRKKYKDVADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSET 300
           S+ RG+KKYKDVADAAQAAFESAA+AA AA+AAVELSQ      D   N+  +   S   
Sbjct: 241 SVKRGKKKYKDVADAAQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGE--NSFHG 300

Query: 301 DESKNTEIELESKNKETHSGSTSIEVEESENETELKQPVSSSSSEAGDASLKENEIPIEA 360
            E+K +E E E  +            + SE E ++      S S++ D      ++P+ +
Sbjct: 301 SENKESEQEQEGND------------DSSEGEGDVMSESKRSMSDSEDII----DVPVMS 360

Query: 361 I--NIIELLEKDLVFDESDVDTGNGESSSSTLEERSSSFQRCEKMESESAEI--TTHVEE 420
              + ++LLEKD +  +S+ +T   + S  T+    S  ++   + S  A+     H+  
Sbjct: 361 FREDPVKLLEKDTIIYDSEEET---QPSVETITISKSKDEQNRMVGSNRADTRHVDHMVR 409

Query: 421 SENKNTAQNVSIGDEAVERTIDVRGH 436
           S      +   +      RT  VRG+
Sbjct: 421 SIEDPIMRKAGLKGPVSVRTRQVRGY 409

BLAST of Lag0040275 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 132.9 bits (333), Expect = 6.3e-31
Identity = 98/298 (32.89%), Postives = 153/298 (51.34%), Query Frame = 0

Query: 5   LDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVE 64
           L+ L  R     + K  +NLAI+R+ +L N+R ++    + ++   LQ G +  A  RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  QVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDFPELV 124
            VI+E  L  AY I+E +CE +L R+  LE  +ECP EL+EAI+ +IFA+ RC + P+L+
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGITL 184
           +I+++  +++GKEF     ELR +  V+  I++KLS   PS   +LK+LK+IA E  +  
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNW 183

Query: 185 KDLEAYEVSTEMQEKVVAEEK---------KDRPQQ----ETKVEGVFQILPEE------ 244
                     +  E ++   K         + RP Q    ++ V    + LP E      
Sbjct: 184 DSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 243

Query: 245 -IEKDNRYADSMRG-------------RKKYKDVADAAQAAFESAAYAAAAARAAVEL 270
            ++  N  + SM               R+   DV + A+AA  SA  A AAARAA +L
Sbjct: 244 KLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQL 301

BLAST of Lag0040275 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 126.3 bits (316), Expect = 5.9e-29
Identity = 112/390 (28.72%), Postives = 188/390 (48.21%), Query Frame = 0

Query: 7   ALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQV 66
           +L  R F +++ K    +A++R+ ++ N+R V   Q R D+  LLQ G    A  RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDFPELVEI 126
           I+EQ +  A  IIE +CE+++ R+  + + ++CP +LKE I+ LIFA+ RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 RSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGITLKD 186
           R +   ++GK+F +   +LR +C V+ M++ KLS R P  E KLK++K+IA E  +    
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDT 199

Query: 187 LEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKD-NRYADSMRGRKKYKDV 246
            E  +   + QE+ +   +K        V       P +  K   R   SM     Y D 
Sbjct: 200 TETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDT 259

Query: 247 ADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETDES------KNT 306
             AA+AA E A  A AAA+ A  L+  +     + S  +S    + + D         + 
Sbjct: 260 ESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFS--VSSDHSTHQKDSQYMDHHHHHP 319

Query: 307 EIELESKNKETHS------------GSTSIEVEESENETELKQPVSSSSSEAGDA----- 366
               +S++ ET S            G          NE++ ++  +++ +EA +      
Sbjct: 320 GSRRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKETMRRRH 379

Query: 367 SLKENEIPIEAINIIELLEKDLVFDESDVD 373
           S     +P  A + I+  E D   +E++ D
Sbjct: 380 SYNPRSVPPPATSEIKFDESDYYEEETEPD 407

BLAST of Lag0040275 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 123.6 bits (309), Expect = 3.8e-28
Identity = 99/341 (29.03%), Postives = 160/341 (46.92%), Query Frame = 0

Query: 8   LLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVEQVI 67
           +L R FK  + K  + +A SRL +L N+++++  Q R ++ QLL+ G    A  RVE V+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 68  KEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDFPELVEIR 127
           +E+K + AY +I  YCE+++ R+  +E  + CP +LKEA++ ++FAS R  D PEL EI 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 128 SVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGIT---- 187
              T+++GK+F+   +ELR +  VS ++V+KLSA+ P    K+K+L  IA E  +     
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

Query: 188 ------------LKDLEAYEVSTEMQEKVVAEEKKDRP---QQETKVEGVFQILPEEIEK 247
                       L    +++ ++ M         K++P        V             
Sbjct: 184 SFVESDPKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHSP 243

Query: 248 DNRYAD----------------------------------------------SMRGRKKY 280
           +N YA+                                              S R ++K+
Sbjct: 244 ENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENSSSRNKQKW 303

BLAST of Lag0040275 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 120.2 bits (300), Expect = 4.2e-27
Identity = 99/325 (30.46%), Postives = 167/325 (51.38%), Query Frame = 0

Query: 5   LDALLGRSFKTNRFKALVNLAISRLAVLANQRQVRSSQARSDVVQLLQLGHQERALFRVE 64
           LD+   + FK  + K L+ L I R+ ++ N+R+ +  Q R ++ +LL+ G +  A  RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  QVIKEQKLLDAYAIIESYCEVVLERIKQLEQGRECPEELKEAISGLIFASSRCGDFPELV 124
            +I+E+K++ A  I+E +CE++  R+  +E  RECP +LKEAIS + FA+ RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIRSVITSRFGKEFTARVIELRNNCAVSPMIVQKLSARPPSLEIKLKLLKQIASEIGITL 184
           +++ +  S++GKEF A   EL+ +  V+  +V+ LS R PS E KLKLLK+IA E     
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEE----- 183

Query: 185 KDLEAYEVSTEMQEKVVAEEKKDRPQQETKVEGVFQILPEEIEKDNRYADSMRGRKKYKD 244
            +L+    STE       E+  D P+Q     G    LPEE + +     S+   K+  D
Sbjct: 184 HELDWDPASTETDLFKSHEDLLDGPKQFG--GGSKLPLPEE-QNEKTNLTSLSAAKEKSD 243

Query: 245 VADAAQAAFESAAYAAAAARAAVELSQSKSLDRDDLSNLISKPRKSSETD-ESKNTEIEL 304
               + + ++   +             + S++  D +   S    S +   +S+N  +E 
Sbjct: 244 ----SDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEK 303

Query: 305 ESKNKETHSGSTSIEVEESENETEL 329
            +  ++ H    S  V E +  + +
Sbjct: 304 TASKRDEHPAKASKTVVEGQQSSPI 316