Lag0040239 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040239
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionIsoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1
Locationchr13: 3163935 .. 3167220 (+)
RNA-Seq ExpressionLag0040239
SyntenyLag0040239
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGTCGCCTCAGATCCTTCCTATAACCCAGCCCCCTTCGTCGTCTCCATTTTCCTCCGAAGGCTCAGCGGCCAATTCTATTATGGCAACAGCATCTACTACCATAGACGTAACGTTGCTTAAAGAAGACGATGAACAGAGAACTGGAGTGCTTGTTTCTCCCTTGTTCGAGGATGATAAGACGGTAGCTTATAAGCCCTTTCTTCCAAGGACTTCAAGCTATGCCTCCACCACATCCAGTTCATCCACCGGAACCATTATGTACAAGCAGAAGCGGAGGCGAGTCAAGAGTGAAGAATTTTTGCCCTTTCTCTCCGGTGACGGCCGTCACCAGACTGTTGATCGTCATGTCGAGAATGCCGATGTCGTGAGAGCTGAGAGATTTCTACTGACTCGGTTTGGTTTGAAACTCTCGAAATATATTAGGTAAAATTGCCTAACTCATTTTCTTTCTGTTAAGAGATCGGTTTCTTAATCCAATCCTCTTCTGCTTTCTGATTAGTGTGTATAAATCAAGATTCATGGACACAAACTTATATATCAAAATCTAGAATATCTGCTTATGGAAAGTTTTTACTTGTATATCATCACCTTTTCTTATTGGGTGGCTTATCATGTTTGGGATTTTACAACATTCTATAATTCAGGGTAGCCATCAGATATATTGCAAGATTTCTCGCACTTGGCTGTTACTCATTGTTTCTTCTCCCAGGTTTTCTTCAAGGTATGCGAGTCAAGATTCTTATTTGTAAGCAGCACATTGCAATATTCATTTTTGCTTTGTGAAGTTAAGTTAGCTGACATATTTCACCCATCGATCATTCTGAACTTTGTTATTGGCAGTTGGATACTATTATTTCTCATCCAGCCAGATACGCAGAAGTATACCCTATGGTGATAAACCAAGGAATAAGTGAGGAACCAATAAACGATGCATATATTTCTCTTATGTGAATTTAAGATGAAAGTGCATGAAGTAATTACAACTTCATATGTTGAGGCATGAAATTTACTGGTGTAGGTTGGATCTTTATCTTCCAAAACATATTGACGGGCCAAAACCAGTTGTTGCATTTATCACTGGCGGGGCTTGGATTATTGGGTAAGCAGTGATATCCTTTGACTGTTCTTAATTTCATGTTCTTCCAGGGGAAGGTGTTGTAAAGCTATTTTAGACAAGACATAACCGATTCTGCTGATACTGACATCTGCTTCGTTATATGGCTGAAATTTCAGCCATGATGTTGAAACCATAAGTTGTTAAAGTGAACATGATAGTAGAAATTATGTTCAATTTGATGATAGAGTTGTCATTTCTGTCGATGCAGGTACAAAGCTTGGGGTTGTCTTCTAGGACAACAATTATCAGAACGAGATGTCATCGTAGCATGCATCGATTACAGGTAATTTGGTCTCAATAATGATGTAATCATCATTGCCATACGTCCATCTCGTCAAATGAAATCCACTTTCAGTGTAGTTAATGATAAATTGTACATACTTTTGATGTAGAAATTTTCCTCAGGGAACCATGAGTGATATGATTGACGATGCTTCCCAAGGTATCTCGTTCTTGTGTAATAACATCAGAGAATTTGGAGGCGATCCAAACAGGTTAATGTTTTACTTTCCCCACCTCCACCTCCACCTCCTGCTATGTTCTGGAGTTAAAAATCTTCAAAAATTAACTTCCTAGGCAGATTCCTTCCATTTAAAGAGATCCAAACACTTCTGATTAGGTTGAGCCATAAATATTGAATCAATTTTTTGAAAGGGTGGATGATCATTTATCAATGAAATTTGTTTCTCTTCTCAAAAAAAAAAAAAAAACAAAAAAAAAAAAGAAAGGGTGGATGATCATGAGAGAGTGGAGAGATAGCTTCATGTCTCTGTGCAAAGCTCCATCCGAAAGAAGAAGAAAAGAGAACTAAGGCTCTTACTATATTCTTATAGTTTTCTCTTTCAATATTAATACAGAATGGCACATTGTTACTATTTTGTCCTTCAAATTCAGATGCCTATTACTGTTGAGTTGTATAGTTCATGATTTGAAGCATTAACTGACTGTGTATTCCTTTTTTTTTTCTTTTTATCTCTTCTAGAATTTTTCTAATGGGTCAGTCAGCTGGCGCTCATATAGCTGCTTGCACACTTTTGGAGCAAGCAATTAAAGAGGTTCGCAGAGTAGAAAGTATCTCATGGAGTGTGTCCCAAATTAAAGCTTACTTTGGTTTGTCAGGAGGGTGAGTATTTCTACCACTATGGATCCAAGAGTTTGGCCATAAAAAGGGTATGCTATGGCATTCCCTTCTATAATAATGTTCTTATAATCATTTTGCTTTCGTATTTGGTGTTTTGTGTTCTTGTAATCATTTTGTGCTCTACATGTAAAAAGGTACAATTTGCTTAATCTGGTAGACTACTTTCACTCTCGAGGTCTATATCGTTCTCTTTTCTAAGGTATTAGAAGTTCTTGTAGTAAATTATTACCATACATCCTTAATTTTGAATTTTTATAATGTAATCAAATTCATTGCAGCATAATGGAAGGAGAACAATCCTTGAAACGATTTTCACCCGAAGTAATGATCCTGGAAGAACCTAACATGGGAGCTGTCGTCTCGATTCTTCCTCCCATCATTTTATTTCATGGAACAGCAGACTATTCTATACCATCAGATGCTAGGTACGTCGTCATGTAGATCTATAAAGGCATATGTAACATAGATATCGATGATGCCTTAAAATTCAAGAAGTTTATCTCCTCTCCGTTTAACCCAAATTTAAAGCATCTCTTGTATTTCAAGCAGTTTTGTTGCCATGCAGTAAAACTTTTGCTGAGACTCTTCAAAGTGTTGGAGTGAAAGCCGAGACATTTTTCTACGAAGGAAAGACACATACAGATGTATTTGTTCAGGTAAAAGAACATTGTTCTTAAGATACTTTGGCAACCTTGTTTACACTTAGAAATGCTACTATGGTGGTTTTGTTTTACACTTCCAGCATCTGCATCTTGTTTATCTAAAAGATTTTGCATAGCAAACTTATTACAGTCACATGAAGTAACGTTTGCTGGCTTCATCAGTTCCCTTTTTGCAATAACCTTAATCTTTCTTCATGCAGGATCCACTAAGGGTGGCAGAGATCAGATGTTTGAAGATCTTGTCGCGATCATCCATGCTAATGATGCAGATGCGCAGGCGAGAGATGCAGAGGCACCTCCAAGAAGACGCCTTGTACCCGAATTTATGTTGATGTTGGCTCGTAGTGTTAGCCCTTTCTAA

mRNA sequence

ATGCCGTCGCCTCAGATCCTTCCTATAACCCAGCCCCCTTCGTCGTCTCCATTTTCCTCCGAAGGCTCAGCGGCCAATTCTATTATGGCAACAGCATCTACTACCATAGACGTAACGTTGCTTAAAGAAGACGATGAACAGAGAACTGGAGTGCTTGTTTCTCCCTTGTTCGAGGATGATAAGACGGTAGCTTATAAGCCCTTTCTTCCAAGGACTTCAAGCTATGCCTCCACCACATCCAGTTCATCCACCGGAACCATTATGTACAAGCAGAAGCGGAGGCGAGTCAAGAGTGAAGAATTTTTGCCCTTTCTCTCCGGTGACGGCCGTCACCAGACTGTTGATCGTCATGTCGAGAATGCCGATGTCGTGAGAGCTGAGAGATTTCTACTGACTCGGTTTGGTTTGAAACTCTCGAAATATATTAGGGTAGCCATCAGATATATTGCAAGATTTCTCGCACTTGGCTGTTACTCATTGTTTCTTCTCCCAGGTTTTCTTCAAGTTGGATACTATTATTTCTCATCCAGCCAGATACGCAGAAGTATACCCTATGGTGATAAACCAAGGAATAAGTTGGATCTTTATCTTCCAAAACATATTGACGGGCCAAAACCAGTTGTTGCATTTATCACTGGCGGGGCTTGGATTATTGGGTACAAAGCTTGGGGTTGTCTTCTAGGACAACAATTATCAGAACGAGATGTCATCGTAGCATGCATCGATTACAGAAATTTTCCTCAGGGAACCATGAGTGATATGATTGACGATGCTTCCCAAGGTATCTCGTTCTTGTGTAATAACATCAGAGAATTTGGAGGCGATCCAAACAGAATTTTTCTAATGGGTCAGTCAGCTGGCGCTCATATAGCTGCTTGCACACTTTTGGAGCAAGCAATTAAAGAGGTTCGCAGAGTAGAAAGTATCTCATGGAGTGTGTCCCAAATTAAAGCTTACTTTGGTTTGTCAGGAGGCATAATGGAAGGAGAACAATCCTTGAAACGATTTTCACCCGAAGTAATGATCCTGGAAGAACCTAACATGGGAGCTGTCGTCTCGATTCTTCCTCCCATCATTTTATTTCATGGAACAGCAGACTATTCTATACCATCAGATGCTAGTAAAACTTTTGCTGAGACTCTTCAAAGTGTTGGAGTGAAAGCCGAGACATTTTTCTACGAAGGAAAGACACATACAGATGTATTTGTTCAGTCACATGAAGTAACGTTTGCTGGCTTCATCAGTTCCCTTTTTGCAATAACCTTAATCTTTCTTCATGCAGGATCCACTAAGGGTGGCAGAGATCAGATGTTTGAAGATCTTGTCGCGATCATCCATGCTAATGATGCAGATGCGCAGGCGAGAGATGCAGAGGCACCTCCAAGAAGACGCCTTGTACCCGAATTTATGTTGATGTTGGCTCGTAGTGTTAGCCCTTTCTAA

Coding sequence (CDS)

ATGCCGTCGCCTCAGATCCTTCCTATAACCCAGCCCCCTTCGTCGTCTCCATTTTCCTCCGAAGGCTCAGCGGCCAATTCTATTATGGCAACAGCATCTACTACCATAGACGTAACGTTGCTTAAAGAAGACGATGAACAGAGAACTGGAGTGCTTGTTTCTCCCTTGTTCGAGGATGATAAGACGGTAGCTTATAAGCCCTTTCTTCCAAGGACTTCAAGCTATGCCTCCACCACATCCAGTTCATCCACCGGAACCATTATGTACAAGCAGAAGCGGAGGCGAGTCAAGAGTGAAGAATTTTTGCCCTTTCTCTCCGGTGACGGCCGTCACCAGACTGTTGATCGTCATGTCGAGAATGCCGATGTCGTGAGAGCTGAGAGATTTCTACTGACTCGGTTTGGTTTGAAACTCTCGAAATATATTAGGGTAGCCATCAGATATATTGCAAGATTTCTCGCACTTGGCTGTTACTCATTGTTTCTTCTCCCAGGTTTTCTTCAAGTTGGATACTATTATTTCTCATCCAGCCAGATACGCAGAAGTATACCCTATGGTGATAAACCAAGGAATAAGTTGGATCTTTATCTTCCAAAACATATTGACGGGCCAAAACCAGTTGTTGCATTTATCACTGGCGGGGCTTGGATTATTGGGTACAAAGCTTGGGGTTGTCTTCTAGGACAACAATTATCAGAACGAGATGTCATCGTAGCATGCATCGATTACAGAAATTTTCCTCAGGGAACCATGAGTGATATGATTGACGATGCTTCCCAAGGTATCTCGTTCTTGTGTAATAACATCAGAGAATTTGGAGGCGATCCAAACAGAATTTTTCTAATGGGTCAGTCAGCTGGCGCTCATATAGCTGCTTGCACACTTTTGGAGCAAGCAATTAAAGAGGTTCGCAGAGTAGAAAGTATCTCATGGAGTGTGTCCCAAATTAAAGCTTACTTTGGTTTGTCAGGAGGCATAATGGAAGGAGAACAATCCTTGAAACGATTTTCACCCGAAGTAATGATCCTGGAAGAACCTAACATGGGAGCTGTCGTCTCGATTCTTCCTCCCATCATTTTATTTCATGGAACAGCAGACTATTCTATACCATCAGATGCTAGTAAAACTTTTGCTGAGACTCTTCAAAGTGTTGGAGTGAAAGCCGAGACATTTTTCTACGAAGGAAAGACACATACAGATGTATTTGTTCAGTCACATGAAGTAACGTTTGCTGGCTTCATCAGTTCCCTTTTTGCAATAACCTTAATCTTTCTTCATGCAGGATCCACTAAGGGTGGCAGAGATCAGATGTTTGAAGATCTTGTCGCGATCATCCATGCTAATGATGCAGATGCGCAGGCGAGAGATGCAGAGGCACCTCCAAGAAGACGCCTTGTACCCGAATTTATGTTGATGTTGGCTCGTAGTGTTAGCCCTTTCTAA

Protein sequence

MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDDKTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGGIMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF
Homology
BLAST of Lag0040239 vs. NCBI nr
Match: XP_038891409.1 (LOW QUALITY PROTEIN: isoprenylcysteine alpha-carbonyl methylesterase ICME [Benincasa hispida])

HSP 1 Score: 806.6 bits (2082), Expect = 1.2e-229
Identity = 419/502 (83.47%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPI QPPSSS FSS GSAANSIMAT ST ID+ LLKEDDEQRTGVLVSPLFE+D
Sbjct: 92  MPSPQILPIAQPPSSSSFSSGGSAANSIMATTSTAIDIILLKEDDEQRTGVLVSPLFEND 151

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+A+KP LPRTSSYAS+TSSSS+G+ MYKQKRRRVKSE+FL FLSGDGRHQTVD  VEN
Sbjct: 152 KTIAHKPLLPRTSSYASSTSSSSSGSNMYKQKRRRVKSEDFLSFLSGDGRHQTVDHDVEN 211

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           ADV RAERFLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 212 ADVERAERFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 271

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC
Sbjct: 272 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 331

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLE A+
Sbjct: 332 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEHAM 391

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 392 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLLDYFHSRGLSRSLFLSIMEGEQSLKRFSP 451

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           EVMILEEPN+GA VSILPP+ILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH
Sbjct: 452 EVMILEEPNIGAAVSILPPVILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 511

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GGRDQMFEDLVAIIHANDA+A A+DA 
Sbjct: 512 TDVFVQD-----------------------PLRGGRDQMFEDLVAIIHANDAEALAKDAV 570

BLAST of Lag0040239 vs. NCBI nr
Match: KAA0034515.1 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 [Cucumis melo var. makuwa] >TYK09069.1 isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 792.3 bits (2045), Expect = 2.3e-225
Identity = 408/502 (81.27%), Postives = 431/502 (85.86%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSP ILPITQPPSSSPFSS GS  NSIMAT ST +D+ +LKEDDE RTGVLVSPLF+DD
Sbjct: 1   MPSPPILPITQPPSSSPFSSGGSVTNSIMATTSTAVDIMVLKEDDEHRTGVLVSPLFDDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           +++A+KP LPRTSSYAS+TS+SS+G+ MYKQKRRRVKSEEFL FLSGDGRHQTVD  VEN
Sbjct: 61  RSIAHKPLLPRTSSYASSTSTSSSGSTMYKQKRRRVKSEEFLSFLSGDGRHQTVDHDVEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           ADV RAERFLLTRFGLKLSKYIRVA R+IARFLALGCYS FLLPGFLQVGYYYFSSSQIR
Sbjct: 121 ADVERAERFLLTRFGLKLSKYIRVAFRWIARFLALGCYSFFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLP+HIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPRHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLE A+
Sbjct: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEHAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLSRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E+MILEEPN+GA VSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH
Sbjct: 361 ELMILEEPNIGAAVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GGRDQMFEDLVAIIHANDA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGRDQMFEDLVAIIHANDAEALAKDAV 479

BLAST of Lag0040239 vs. NCBI nr
Match: XP_022957176.1 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 [Cucurbita moschata] >KAG7032117.1 putative isoprenylcysteine alpha-carbonyl methylesterase ICMEL1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 791.2 bits (2042), Expect = 5.0e-225
Identity = 408/502 (81.27%), Postives = 433/502 (86.25%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPITQPPSSSP +S  S ANSIM T+ST +D+ LLKEDDEQRTGVLVSPLFEDD
Sbjct: 1   MPSPQILPITQPPSSSPLTSRASDANSIMETSSTALDIMLLKEDDEQRTGVLVSPLFEDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+AYKP LPRTSSYAS+TSSSS+G+  YKQKRRRVKSE FL +LSGDGRHQT+DR VEN
Sbjct: 61  KTIAYKPLLPRTSSYASSTSSSSSGSNTYKQKRRRVKSENFLSYLSGDGRHQTIDRDVEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           AD  RAE+FLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 121 ADADRAEKFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSER +IVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERGIIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMI DASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLEQA+
Sbjct: 241 IDYRNFPQGTMSDMIYDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEQAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLYRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E++ILEEPNMGA VSILPPIILFHGTADYSIPSD+SKTFA+TLQSVGVKAETFFYEGKTH
Sbjct: 361 ELLILEEPNMGAAVSILPPIILFHGTADYSIPSDSSKTFADTLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GG+DQMFEDLVAIIHA+DA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGKDQMFEDLVAIIHADDAEALAKDAV 479

BLAST of Lag0040239 vs. NCBI nr
Match: XP_004135123.2 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 [Cucumis sativus] >KGN52032.1 hypothetical protein Csa_008383 [Cucumis sativus])

HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 408/502 (81.27%), Postives = 427/502 (85.06%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSP ILPITQPPSSSPFSS GS  NSIMAT ST ID+ LLKEDDE RTG+LVSPLF+DD
Sbjct: 86  MPSPSILPITQPPSSSPFSSGGSVTNSIMATTSTAIDIMLLKEDDEHRTGLLVSPLFDDD 145

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           +++ +KP LPRTSSYAS+TS+SS+G+ MYKQKRRRVKSEEFL FLSGDGRHQT D  VEN
Sbjct: 146 RSIGHKPLLPRTSSYASSTSTSSSGSTMYKQKRRRVKSEEFLSFLSGDGRHQTFDHDVEN 205

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           ADV RAERFLLTR GLKLSKYIRVA R+IARFLALGCYS FLLPGFLQVGYYYFSSSQIR
Sbjct: 206 ADVERAERFLLTRLGLKLSKYIRVAFRWIARFLALGCYSFFLLPGFLQVGYYYFSSSQIR 265

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC
Sbjct: 266 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 325

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLE A+
Sbjct: 326 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEHAM 385

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 386 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLSRSLFLSIMEGEQSLKRFSP 445

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           EVMILEEPN+GA VSILPPIILFHGTADYSIPSDASKTFAETLQSVGVK ETFFYEGKTH
Sbjct: 446 EVMILEEPNIGAAVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKTETFFYEGKTH 505

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GGRDQMFEDLV IIHANDA+A A+DA 
Sbjct: 506 TDVFVQD-----------------------PLRGGRDQMFEDLVGIIHANDAEALAKDAV 564

BLAST of Lag0040239 vs. NCBI nr
Match: XP_022977831.1 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 [Cucurbita maxima])

HSP 1 Score: 787.3 bits (2032), Expect = 7.3e-224
Identity = 405/502 (80.68%), Postives = 432/502 (86.06%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPITQPPSSSP +S  S ANSIM T+ST +D+ LLKEDDEQRTGVLVSPLFEDD
Sbjct: 1   MPSPQILPITQPPSSSPLTSRASDANSIMETSSTALDIMLLKEDDEQRTGVLVSPLFEDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+AYKP LPRTSSYAS+TSSSS+G+  YKQKRRRVKSE FL +LSGDGRHQT+DR +EN
Sbjct: 61  KTIAYKPLLPRTSSYASSTSSSSSGSNTYKQKRRRVKSENFLSYLSGDGRHQTIDRDIEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
            +  RAE+FLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 121 GNADRAEKFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSER +IVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERGIIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMI DASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLEQA+
Sbjct: 241 IDYRNFPQGTMSDMIYDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEQAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLYRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E++ILEEPNMGA VSILPPIILFHGTADYSIPSD+SKTFA+TLQSVGVKAETFFYEGKTH
Sbjct: 361 ELLILEEPNMGAAVSILPPIILFHGTADYSIPSDSSKTFADTLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GG+DQMFEDLVAIIHA+DA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGKDQMFEDLVAIIHADDAEALAKDAV 479

BLAST of Lag0040239 vs. ExPASy Swiss-Prot
Match: Q8VYP9 (Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1 OS=Arabidopsis thaliana OX=3702 GN=ICMEL1 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.7e-130
Identity = 272/521 (52.21%), Postives = 335/521 (64.30%), Query Frame = 0

Query: 1   MPSPQILPITQ--PPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFE 60
           MPS QIL I+   PP SSP               ST +    L  DD   T  L+S   +
Sbjct: 1   MPS-QILQISHHLPPKSSP---------------STEMMFKSLIYDDPSTT--LLSRFGD 60

Query: 61  DDKTVA--YKPFLPRTSSY----ASTTSSSSTG--TIMYKQKRRRVKSEEFLPFLS---- 120
           D  T++   KP L R+SSY      T+SSSS G  T  Y+ +RRR  S+  L   S    
Sbjct: 61  DHHTISSTVKPLLSRSSSYNGTAMKTSSSSSAGGFTGWYQNRRRRSNSDNCLSAFSDDTN 120

Query: 121 --GDGRHQTVDRHV---ENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLF 180
              DG + + DR     +      AE FLLTR  LKL  Y+ V  R+I RF+ALGCY+  
Sbjct: 121 GTADGGNNSGDRQTTIGQEVGHAAAETFLLTRLCLKLLSYLGVGYRWITRFMALGCYAFL 180

Query: 181 LLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYK 240
           L+PGF+QVGYYYF S  +RRSI YGD+PRN+LDLYLPK+  GPKPVVAF+TGGAWIIGYK
Sbjct: 181 LMPGFIQVGYYYFFSPYVRRSIVYGDQPRNRLDLYLPKNSTGPKPVVAFVTGGAWIIGYK 240

Query: 241 AWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFL 300
           AWG LLGQQLSERD+IVACIDYRNFPQG++SDM+ DAS GISF+CN+I E+GGDP+RI+L
Sbjct: 241 AWGSLLGQQLSERDIIVACIDYRNFPQGSISDMVKDASSGISFVCNHIAEYGGDPDRIYL 300

Query: 301 MGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG---------------- 360
           MGQSAGAHIAACT++EQ IKE    +S+SWS SQI AYFGLSGG                
Sbjct: 301 MGQSAGAHIAACTIVEQVIKESGEGDSVSWSSSQINAYFGLSGGYNLLNLVDHFHSRGLY 360

Query: 361 ------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAE 420
                 IMEGE+SL++FSPE ++++ PN+  +++ LPP ILFHGT DYSIPSDASK+FAE
Sbjct: 361 RSIFLSIMEGEESLRQFSPE-LVVQNPNLKHIIARLPPFILFHGTDDYSIPSDASKSFAE 420

Query: 421 TLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFE 480
           TLQ +G KA+   YEGKTHTD+F+Q                          +GG D+MFE
Sbjct: 421 TLQRLGAKAKVILYEGKTHTDLFLQD-----------------------PMRGGIDEMFE 476

BLAST of Lag0040239 vs. ExPASy Swiss-Prot
Match: Q94AS5 (Isoprenylcysteine alpha-carbonyl methylesterase ICME OS=Arabidopsis thaliana OX=3702 GN=ICME PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 5.2e-124
Identity = 226/393 (57.51%), Postives = 280/393 (71.25%), Query Frame = 0

Query: 110 RHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQV 169
           R Q+  R + +A    AE +L+T    KL +Y+ V  R++ + LAL CY++ L+PGFLQV
Sbjct: 63  RQQSFGRDIGHA---AAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQV 122

Query: 170 GYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQ 229
            Y YF S Q+RRSI YGD+PRN+LDLYLP + DG KPVV F+TGGAWIIGYKAWG LLG 
Sbjct: 123 AYSYFFSKQVRRSIVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGSLLGM 182

Query: 230 QLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAH 289
           QL+ERD+IVAC+DYRNFPQGT+SDM+ DASQGISF+CNNI  FGGDPNRI+LMGQSAGAH
Sbjct: 183 QLAERDIIVACLDYRNFPQGTISDMVTDASQGISFVCNNISAFGGDPNRIYLMGQSAGAH 242

Query: 290 IAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG----------------------IM 349
           IAAC LLEQA KE++  ESISW+VSQIKAYFGLSGG                      IM
Sbjct: 243 IAACALLEQATKELKG-ESISWTVSQIKAYFGLSGGYNLYKLVDHFHNRGLYRSIFLSIM 302

Query: 350 EGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVK 409
           EGE+S ++FSPEV  L++P +G   S+LPPIILFHG++DYSIP D SKTF + LQ+VG K
Sbjct: 303 EGEESFEKFSPEVR-LKDPVVGKAASLLPPIILFHGSSDYSIPCDESKTFTDALQAVGAK 362

Query: 410 AETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHA 469
           AE   Y GKTHTD+F+Q                          +GG+D++F+D+V++IHA
Sbjct: 363 AELVLYSGKTHTDLFLQD-----------------------PLRGGKDELFDDIVSVIHA 422

Query: 470 NDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
            D D   +D+ APPR+RLVPE +L LAR +SPF
Sbjct: 423 EDNDGLTKDSLAPPRKRLVPELLLKLAREISPF 427

BLAST of Lag0040239 vs. ExPASy Swiss-Prot
Match: Q1PET6 (Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL2 OS=Arabidopsis thaliana OX=3702 GN=ICMEL2 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.2e-120
Identity = 233/413 (56.42%), Postives = 285/413 (69.01%), Query Frame = 0

Query: 93  RRRV---KSEEFLPFLSGDGRHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYI 152
           RRRV    SE+  P +    R Q+  R + +A    AE +L+TR    L  Y+ V  R+I
Sbjct: 41  RRRVSGNSSEDGSPRIC---RQQSFGRDIGHA---AAETYLITRLSFNLLGYLGVGYRWI 100

Query: 153 ARFLALGCYSLFLLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVA 212
            R LAL CY++ L+PGFLQV Y YF SSQ+RRSI YG  PRN+LDLY+P   DG KPVV 
Sbjct: 101 TRLLALACYAMLLMPGFLQVAYLYFFSSQVRRSIVYGGHPRNRLDLYIPPTSDGLKPVVV 160

Query: 213 FITGGAWIIGYKAWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNI 272
           F+TGGAWIIGYKAWG LLG QL+ERD+IVAC+DYRNFPQGT+SDM+ DA+QGISF+CNNI
Sbjct: 161 FVTGGAWIIGYKAWGSLLGLQLAERDIIVACLDYRNFPQGTISDMVSDAAQGISFVCNNI 220

Query: 273 REFGGDPNRIFLMGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG---- 332
             FGGDPNRI+LMGQSAGAHI++C L EQAIKE  R ESISWSVSQIKAYFGLSGG    
Sbjct: 221 SAFGGDPNRIYLMGQSAGAHISSCALFEQAIKE-SRGESISWSVSQIKAYFGLSGGYNLF 280

Query: 333 ------------------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADY 392
                             IMEGE+S K+FSPEV  L++ N+    ++LP IILFHG+ADY
Sbjct: 281 NLVEHFHNRGLYRSIFLSIMEGEESFKQFSPEVR-LKDLNVRKAAALLPHIILFHGSADY 340

Query: 393 SIPSDASKTFAETLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHA 452
           SIP +ASKTF + LQ+  VKAE   Y+GKTHTD+F+Q                       
Sbjct: 341 SIPPEASKTFTDALQAAEVKAELVMYKGKTHTDLFLQD---------------------- 400

Query: 453 GSTKGGRDQMFEDLVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
              +GG+D++F+ +V++IHA+D+DA   DA APPR+RLVPEF+L LA  VSPF
Sbjct: 401 -PLRGGKDELFDHIVSMIHADDSDALRNDAVAPPRKRLVPEFLLKLAGRVSPF 422

BLAST of Lag0040239 vs. ExPASy Swiss-Prot
Match: Q5Z9I2 (Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1 OS=Oryza sativa subsp. japonica OX=39947 GN=IMCEL1 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 4.6e-112
Identity = 213/410 (51.95%), Postives = 274/410 (66.83%), Query Frame = 0

Query: 93  RRRVKSEEFLPFLSGDGRHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARF 152
           RRRV   E     SG  R + V R  E       E +LLTR  L L +Y+ +  R+I +F
Sbjct: 48  RRRVVVSERFRQRSGSFRRE-VRRAAE-------ETYLLTRLTLILLRYLGIGYRWIRQF 107

Query: 153 LALGCYSLFLLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFIT 212
           LAL CY+  L+PGF+QV YYYF SSQ+ RS+ YG++PRN+LDLY+P    G KPVVAF+T
Sbjct: 108 LALCCYTFLLMPGFIQVVYYYFFSSQVCRSVVYGEQPRNRLDLYIPTDRTGLKPVVAFVT 167

Query: 213 GGAWIIGYKAWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREF 272
           GGAWIIGYK WG LLG++L+ER ++VACIDYRNFPQGT+ DM++DASQGI+F+CNNI  +
Sbjct: 168 GGAWIIGYKGWGALLGRRLAERGILVACIDYRNFPQGTIGDMVEDASQGIAFVCNNIASY 227

Query: 273 GGDPNRIFLMGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG------- 332
           GGDP RI+L+GQSAGAHIAACTLL QAIKE    ++ +WS++Q+KAYFG+SGG       
Sbjct: 228 GGDPERIYLVGQSAGAHIAACTLLHQAIKESGEGDASTWSIAQLKAYFGISGGYNLLNLV 287

Query: 333 ---------------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIP 392
                          IMEGE+SL++FSP VM+ ++P   + VS+LP I LFHGT+DYSIP
Sbjct: 288 DHFHKRGLYRSIFLSIMEGEESLQKFSPLVMV-KDPAARSAVSLLPRIFLFHGTSDYSIP 347

Query: 393 SDASKTFAETLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGST 452
           S  S+ F + LQ  G KA+ F Y+GKTHTD+F+Q                          
Sbjct: 348 SAESEAFFDALQQNGAKADLFLYDGKTHTDLFLQD-----------------------PL 407

Query: 453 KGGRDQMFEDLVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
           +GGRD++ E++V +IH ++ D  A+    P  RRLVPEFMLMLA  VSPF
Sbjct: 408 RGGRDKLLEEIVTVIHNDNPDTSAQHLAVPVARRLVPEFMLMLAGRVSPF 425

BLAST of Lag0040239 vs. ExPASy Swiss-Prot
Match: Q6L5F5 (Probable isoprenylcysteine alpha-carbonyl methylesterase ICME OS=Oryza sativa subsp. japonica OX=39947 GN=IMCE PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.9e-108
Identity = 205/400 (51.25%), Postives = 264/400 (66.00%), Query Frame = 0

Query: 103 PFLSGDGRHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFL 162
           P   G G  ++  R  E+     AE +L+TR    L +Y+ V  R+I++  AL  Y++ L
Sbjct: 43  PGSRGGGERRSTFR--EDVSHAAAETYLVTRLAFILLRYLGVGYRWISQLAALIIYAILL 102

Query: 163 LPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKA 222
           +PGF++VGYYYF S Q+ RS+ YGD+PRN+LDLY+P+    P PVVAF+TGGAWIIGYKA
Sbjct: 103 MPGFIRVGYYYFFSRQVLRSVIYGDQPRNRLDLYIPRDPKKPSPVVAFVTGGAWIIGYKA 162

Query: 223 WGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLM 282
           WG LLG++L+ER +IVACIDYRNFPQGT+SDM+ DAS GISF+C  +  +GGDPN+I+LM
Sbjct: 163 WGALLGRRLAERGIIVACIDYRNFPQGTISDMVSDASDGISFVCETVGAYGGDPNQIYLM 222

Query: 283 GQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG----------------- 342
           GQSAGAHIAAC LLEQA KE  R E ISWSV+QIKAYFGLSGG                 
Sbjct: 223 GQSAGAHIAACALLEQAAKE-SRGEQISWSVTQIKAYFGLSGGYNIENLVDHFHERGLYR 282

Query: 343 -----IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAET 402
                IMEG++SL  FSPE   + +      +++LP I+L HGT DYSIP  AS+TFA  
Sbjct: 283 SIFLSIMEGKKSLPHFSPET--VAKKLCPETIALLPQIVLLHGTDDYSIPFSASETFAGV 342

Query: 403 LQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFED 462
           L+  G KA+   YEGKTHTDVF+Q                          +GGRD++ ED
Sbjct: 343 LKQAGAKAKLLLYEGKTHTDVFLQD-----------------------PLRGGRDKLVED 402

Query: 463 LVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
           ++++IHA+DADA+ +DA AP   RLV E+ + LA  +SPF
Sbjct: 403 VISVIHADDADAREKDALAPIPGRLVSEWQIKLAHRISPF 414

BLAST of Lag0040239 vs. ExPASy TrEMBL
Match: A0A5D3CFI9 (Isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00590 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.1e-225
Identity = 408/502 (81.27%), Postives = 431/502 (85.86%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSP ILPITQPPSSSPFSS GS  NSIMAT ST +D+ +LKEDDE RTGVLVSPLF+DD
Sbjct: 1   MPSPPILPITQPPSSSPFSSGGSVTNSIMATTSTAVDIMVLKEDDEHRTGVLVSPLFDDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           +++A+KP LPRTSSYAS+TS+SS+G+ MYKQKRRRVKSEEFL FLSGDGRHQTVD  VEN
Sbjct: 61  RSIAHKPLLPRTSSYASSTSTSSSGSTMYKQKRRRVKSEEFLSFLSGDGRHQTVDHDVEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           ADV RAERFLLTRFGLKLSKYIRVA R+IARFLALGCYS FLLPGFLQVGYYYFSSSQIR
Sbjct: 121 ADVERAERFLLTRFGLKLSKYIRVAFRWIARFLALGCYSFFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLP+HIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPRHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLE A+
Sbjct: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEHAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLSRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E+MILEEPN+GA VSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH
Sbjct: 361 ELMILEEPNIGAAVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GGRDQMFEDLVAIIHANDA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGRDQMFEDLVAIIHANDAEALAKDAV 479

BLAST of Lag0040239 vs. ExPASy TrEMBL
Match: A0A6J1GYH9 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458648 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.4e-225
Identity = 408/502 (81.27%), Postives = 433/502 (86.25%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPITQPPSSSP +S  S ANSIM T+ST +D+ LLKEDDEQRTGVLVSPLFEDD
Sbjct: 1   MPSPQILPITQPPSSSPLTSRASDANSIMETSSTALDIMLLKEDDEQRTGVLVSPLFEDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+AYKP LPRTSSYAS+TSSSS+G+  YKQKRRRVKSE FL +LSGDGRHQT+DR VEN
Sbjct: 61  KTIAYKPLLPRTSSYASSTSSSSSGSNTYKQKRRRVKSENFLSYLSGDGRHQTIDRDVEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           AD  RAE+FLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 121 ADADRAEKFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSER +IVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERGIIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMI DASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLEQA+
Sbjct: 241 IDYRNFPQGTMSDMIYDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEQAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLYRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E++ILEEPNMGA VSILPPIILFHGTADYSIPSD+SKTFA+TLQSVGVKAETFFYEGKTH
Sbjct: 361 ELLILEEPNMGAAVSILPPIILFHGTADYSIPSDSSKTFADTLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GG+DQMFEDLVAIIHA+DA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGKDQMFEDLVAIIHADDAEALAKDAV 479

BLAST of Lag0040239 vs. ExPASy TrEMBL
Match: A0A0A0KUE3 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608200 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.4e-225
Identity = 408/502 (81.27%), Postives = 427/502 (85.06%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSP ILPITQPPSSSPFSS GS  NSIMAT ST ID+ LLKEDDE RTG+LVSPLF+DD
Sbjct: 86  MPSPSILPITQPPSSSPFSSGGSVTNSIMATTSTAIDIMLLKEDDEHRTGLLVSPLFDDD 145

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           +++ +KP LPRTSSYAS+TS+SS+G+ MYKQKRRRVKSEEFL FLSGDGRHQT D  VEN
Sbjct: 146 RSIGHKPLLPRTSSYASSTSTSSSGSTMYKQKRRRVKSEEFLSFLSGDGRHQTFDHDVEN 205

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           ADV RAERFLLTR GLKLSKYIRVA R+IARFLALGCYS FLLPGFLQVGYYYFSSSQIR
Sbjct: 206 ADVERAERFLLTRLGLKLSKYIRVAFRWIARFLALGCYSFFLLPGFLQVGYYYFSSSQIR 265

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC
Sbjct: 266 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 325

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLE A+
Sbjct: 326 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEHAM 385

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 386 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLSRSLFLSIMEGEQSLKRFSP 445

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           EVMILEEPN+GA VSILPPIILFHGTADYSIPSDASKTFAETLQSVGVK ETFFYEGKTH
Sbjct: 446 EVMILEEPNIGAAVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKTETFFYEGKTH 505

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GGRDQMFEDLV IIHANDA+A A+DA 
Sbjct: 506 TDVFVQD-----------------------PLRGGRDQMFEDLVGIIHANDAEALAKDAV 564

BLAST of Lag0040239 vs. ExPASy TrEMBL
Match: A0A6J1IL23 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477934 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.5e-224
Identity = 405/502 (80.68%), Postives = 432/502 (86.06%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPITQPPSSSP +S  S ANSIM T+ST +D+ LLKEDDEQRTGVLVSPLFEDD
Sbjct: 1   MPSPQILPITQPPSSSPLTSRASDANSIMETSSTALDIMLLKEDDEQRTGVLVSPLFEDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+AYKP LPRTSSYAS+TSSSS+G+  YKQKRRRVKSE FL +LSGDGRHQT+DR +EN
Sbjct: 61  KTIAYKPLLPRTSSYASSTSSSSSGSNTYKQKRRRVKSENFLSYLSGDGRHQTIDRDIEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
            +  RAE+FLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 121 GNADRAEKFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSER +IVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERGIIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMI DASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLEQA+
Sbjct: 241 IDYRNFPQGTMSDMIYDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEQAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLYRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E++ILEEPNMGA VSILPPIILFHGTADYSIPSD+SKTFA+TLQSVGVKAETFFYEGKTH
Sbjct: 361 ELLILEEPNMGAAVSILPPIILFHGTADYSIPSDSSKTFADTLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 480
           TDVFVQ                          +GG+DQMFEDLVAIIHA+DA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGKDQMFEDLVAIIHADDAEALAKDAV 479

BLAST of Lag0040239 vs. ExPASy TrEMBL
Match: A0A6J1GZT4 (isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458648 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.6e-223
Identity = 404/498 (81.12%), Postives = 429/498 (86.14%), Query Frame = 0

Query: 1   MPSPQILPITQPPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFEDD 60
           MPSPQILPITQPPSSSP +S  S ANSIM T+ST +D+ LLKEDDEQRTGVLVSPLFEDD
Sbjct: 1   MPSPQILPITQPPSSSPLTSRASDANSIMETSSTALDIMLLKEDDEQRTGVLVSPLFEDD 60

Query: 61  KTVAYKPFLPRTSSYASTTSSSSTGTIMYKQKRRRVKSEEFLPFLSGDGRHQTVDRHVEN 120
           KT+AYKP LPRTSSYAS+TSSSS+G+  YKQKRRRVKSE FL +LSGDGRHQT+DR VEN
Sbjct: 61  KTIAYKPLLPRTSSYASSTSSSSSGSNTYKQKRRRVKSENFLSYLSGDGRHQTIDRDVEN 120

Query: 121 ADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180
           AD  RAE+FLLTRFGLKLSKYIRVA R+IARFLALGCYSLFLLPGFLQVGYYYFSSSQIR
Sbjct: 121 ADADRAEKFLLTRFGLKLSKYIRVAFRWIARFLALGCYSLFLLPGFLQVGYYYFSSSQIR 180

Query: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERDVIVAC 240
           RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSER +IVAC
Sbjct: 181 RSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQQLSERGIIVAC 240

Query: 241 IDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAI 300
           IDYRNFPQGTMSDMI DASQGISFLCNNIREFGGDPNRI+LMGQSAGAHIAACTLLEQA+
Sbjct: 241 IDYRNFPQGTMSDMIYDASQGISFLCNNIREFGGDPNRIYLMGQSAGAHIAACTLLEQAM 300

Query: 301 KEVRRVESISWSVSQIKAYFGLSGG----------------------IMEGEQSLKRFSP 360
           KEVR+VESISWSVSQIKAYFGLSGG                      IMEGEQSLKRFSP
Sbjct: 301 KEVRKVESISWSVSQIKAYFGLSGGYNLLNLVDYFHSRGLYRSLFLSIMEGEQSLKRFSP 360

Query: 361 EVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVKAETFFYEGKTH 420
           E++ILEEPNMGA VSILPPIILFHGTADYSIPSD+SKTFA+TLQSVGVKAETFFYEGKTH
Sbjct: 361 ELLILEEPNMGAAVSILPPIILFHGTADYSIPSDSSKTFADTLQSVGVKAETFFYEGKTH 420

Query: 421 TDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHANDADAQARDAE 477
           TDVFVQ                          +GG+DQMFEDLVAIIHA+DA+A A+DA 
Sbjct: 421 TDVFVQD-----------------------PLRGGKDQMFEDLVAIIHADDAEALAKDAV 475

BLAST of Lag0040239 vs. TAIR 10
Match: AT1G26120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 467.6 bits (1202), Expect = 1.2e-131
Identity = 272/521 (52.21%), Postives = 335/521 (64.30%), Query Frame = 0

Query: 1   MPSPQILPITQ--PPSSSPFSSEGSAANSIMATASTTIDVTLLKEDDEQRTGVLVSPLFE 60
           MPS QIL I+   PP SSP               ST +    L  DD   T  L+S   +
Sbjct: 1   MPS-QILQISHHLPPKSSP---------------STEMMFKSLIYDDPSTT--LLSRFGD 60

Query: 61  DDKTVA--YKPFLPRTSSY----ASTTSSSSTG--TIMYKQKRRRVKSEEFLPFLS---- 120
           D  T++   KP L R+SSY      T+SSSS G  T  Y+ +RRR  S+  L   S    
Sbjct: 61  DHHTISSTVKPLLSRSSSYNGTAMKTSSSSSAGGFTGWYQNRRRRSNSDNCLSAFSDDTN 120

Query: 121 --GDGRHQTVDRHV---ENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLF 180
              DG + + DR     +      AE FLLTR  LKL  Y+ V  R+I RF+ALGCY+  
Sbjct: 121 GTADGGNNSGDRQTTIGQEVGHAAAETFLLTRLCLKLLSYLGVGYRWITRFMALGCYAFL 180

Query: 181 LLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYK 240
           L+PGF+QVGYYYF S  +RRSI YGD+PRN+LDLYLPK+  GPKPVVAF+TGGAWIIGYK
Sbjct: 181 LMPGFIQVGYYYFFSPYVRRSIVYGDQPRNRLDLYLPKNSTGPKPVVAFVTGGAWIIGYK 240

Query: 241 AWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFL 300
           AWG LLGQQLSERD+IVACIDYRNFPQG++SDM+ DAS GISF+CN+I E+GGDP+RI+L
Sbjct: 241 AWGSLLGQQLSERDIIVACIDYRNFPQGSISDMVKDASSGISFVCNHIAEYGGDPDRIYL 300

Query: 301 MGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG---------------- 360
           MGQSAGAHIAACT++EQ IKE    +S+SWS SQI AYFGLSGG                
Sbjct: 301 MGQSAGAHIAACTIVEQVIKESGEGDSVSWSSSQINAYFGLSGGYNLLNLVDHFHSRGLY 360

Query: 361 ------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAE 420
                 IMEGE+SL++FSPE ++++ PN+  +++ LPP ILFHGT DYSIPSDASK+FAE
Sbjct: 361 RSIFLSIMEGEESLRQFSPE-LVVQNPNLKHIIARLPPFILFHGTDDYSIPSDASKSFAE 420

Query: 421 TLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFE 480
           TLQ +G KA+   YEGKTHTD+F+Q                          +GG D+MFE
Sbjct: 421 TLQRLGAKAKVILYEGKTHTDLFLQD-----------------------PMRGGIDEMFE 476

BLAST of Lag0040239 vs. TAIR 10
Match: AT5G15860.1 (prenylcysteine methylesterase )

HSP 1 Score: 446.0 bits (1146), Expect = 3.7e-125
Identity = 226/393 (57.51%), Postives = 280/393 (71.25%), Query Frame = 0

Query: 110 RHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQV 169
           R Q+  R + +A    AE +L+T    KL +Y+ V  R++ + LAL CY++ L+PGFLQV
Sbjct: 63  RQQSFGRDIGHA---AAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQV 122

Query: 170 GYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQ 229
            Y YF S Q+RRSI YGD+PRN+LDLYLP + DG KPVV F+TGGAWIIGYKAWG LLG 
Sbjct: 123 AYSYFFSKQVRRSIVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGSLLGM 182

Query: 230 QLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAH 289
           QL+ERD+IVAC+DYRNFPQGT+SDM+ DASQGISF+CNNI  FGGDPNRI+LMGQSAGAH
Sbjct: 183 QLAERDIIVACLDYRNFPQGTISDMVTDASQGISFVCNNISAFGGDPNRIYLMGQSAGAH 242

Query: 290 IAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG----------------------IM 349
           IAAC LLEQA KE++  ESISW+VSQIKAYFGLSGG                      IM
Sbjct: 243 IAACALLEQATKELKG-ESISWTVSQIKAYFGLSGGYNLYKLVDHFHNRGLYRSIFLSIM 302

Query: 350 EGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADYSIPSDASKTFAETLQSVGVK 409
           EGE+S ++FSPEV  L++P +G   S+LPPIILFHG++DYSIP D SKTF + LQ+VG K
Sbjct: 303 EGEESFEKFSPEVR-LKDPVVGKAASLLPPIILFHGSSDYSIPCDESKTFTDALQAVGAK 362

Query: 410 AETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHAGSTKGGRDQMFEDLVAIIHA 469
           AE   Y GKTHTD+F+Q                          +GG+D++F+D+V++IHA
Sbjct: 363 AELVLYSGKTHTDLFLQD-----------------------PLRGGKDELFDDIVSVIHA 422

Query: 470 NDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
            D D   +D+ APPR+RLVPE +L LAR +SPF
Sbjct: 423 EDNDGLTKDSLAPPRKRLVPELLLKLAREISPF 427

BLAST of Lag0040239 vs. TAIR 10
Match: AT3G02410.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 8.5e-122
Identity = 233/413 (56.42%), Postives = 285/413 (69.01%), Query Frame = 0

Query: 93  RRRV---KSEEFLPFLSGDGRHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYI 152
           RRRV    SE+  P +    R Q+  R + +A    AE +L+TR    L  Y+ V  R+I
Sbjct: 41  RRRVSGNSSEDGSPRIC---RQQSFGRDIGHA---AAETYLITRLSFNLLGYLGVGYRWI 100

Query: 153 ARFLALGCYSLFLLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVA 212
            R LAL CY++ L+PGFLQV Y YF SSQ+RRSI YG  PRN+LDLY+P   DG KPVV 
Sbjct: 101 TRLLALACYAMLLMPGFLQVAYLYFFSSQVRRSIVYGGHPRNRLDLYIPPTSDGLKPVVV 160

Query: 213 FITGGAWIIGYKAWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNI 272
           F+TGGAWIIGYKAWG LLG QL+ERD+IVAC+DYRNFPQGT+SDM+ DA+QGISF+CNNI
Sbjct: 161 FVTGGAWIIGYKAWGSLLGLQLAERDIIVACLDYRNFPQGTISDMVSDAAQGISFVCNNI 220

Query: 273 REFGGDPNRIFLMGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG---- 332
             FGGDPNRI+LMGQSAGAHI++C L EQAIKE  R ESISWSVSQIKAYFGLSGG    
Sbjct: 221 SAFGGDPNRIYLMGQSAGAHISSCALFEQAIKE-SRGESISWSVSQIKAYFGLSGGYNLF 280

Query: 333 ------------------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGTADY 392
                             IMEGE+S K+FSPEV  L++ N+    ++LP IILFHG+ADY
Sbjct: 281 NLVEHFHNRGLYRSIFLSIMEGEESFKQFSPEVR-LKDLNVRKAAALLPHIILFHGSADY 340

Query: 393 SIPSDASKTFAETLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIFLHA 452
           SIP +ASKTF + LQ+  VKAE   Y+GKTHTD+F+Q                       
Sbjct: 341 SIPPEASKTFTDALQAAEVKAELVMYKGKTHTDLFLQD---------------------- 400

Query: 453 GSTKGGRDQMFEDLVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
              +GG+D++F+ +V++IHA+D+DA   DA APPR+RLVPEF+L LA  VSPF
Sbjct: 401 -PLRGGKDELFDHIVSMIHADDSDALRNDAVAPPRKRLVPEFLLKLAGRVSPF 422

BLAST of Lag0040239 vs. TAIR 10
Match: AT3G02410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 4.8e-117
Identity = 214/356 (60.11%), Postives = 258/356 (72.47%), Query Frame = 0

Query: 147 RYIARFLALGCYSLFLLPGFLQVGYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKP 206
           R+I R LAL CY++ L+PGFLQV Y YF SSQ+RRSI YG  PRN+LDLY+P   DG KP
Sbjct: 23  RWITRLLALACYAMLLMPGFLQVAYLYFFSSQVRRSIVYGGHPRNRLDLYIPPTSDGLKP 82

Query: 207 VVAFITGGAWIIGYKAWGCLLGQQLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLC 266
           VV F+TGGAWIIGYKAWG LLG QL+ERD+IVAC+DYRNFPQGT+SDM+ DA+QGISF+C
Sbjct: 83  VVVFVTGGAWIIGYKAWGSLLGLQLAERDIIVACLDYRNFPQGTISDMVSDAAQGISFVC 142

Query: 267 NNIREFGGDPNRIFLMGQSAGAHIAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGG- 326
           NNI  FGGDPNRI+LMGQSAGAHI++C L EQAIKE  R ESISWSVSQIKAYFGLSGG 
Sbjct: 143 NNISAFGGDPNRIYLMGQSAGAHISSCALFEQAIKE-SRGESISWSVSQIKAYFGLSGGY 202

Query: 327 ---------------------IMEGEQSLKRFSPEVMILEEPNMGAVVSILPPIILFHGT 386
                                IMEGE+S K+FSPEV  L++ N+    ++LP IILFHG+
Sbjct: 203 NLFNLVEHFHNRGLYRSIFLSIMEGEESFKQFSPEVR-LKDLNVRKAAALLPHIILFHGS 262

Query: 387 ADYSIPSDASKTFAETLQSVGVKAETFFYEGKTHTDVFVQSHEVTFAGFISSLFAITLIF 446
           ADYSIP +ASKTF + LQ+  VKAE   Y+GKTHTD+F+Q                    
Sbjct: 263 ADYSIPPEASKTFTDALQAAEVKAELVMYKGKTHTDLFLQD------------------- 322

Query: 447 LHAGSTKGGRDQMFEDLVAIIHANDADAQARDAEAPPRRRLVPEFMLMLARSVSPF 481
                 +GG+D++F+ +V++IHA+D+DA   DA APPR+RLVPEF+L LA  VSPF
Sbjct: 323 ----PLRGGKDELFDHIVSMIHADDSDALRNDAVAPPRKRLVPEFLLKLAGRVSPF 353

BLAST of Lag0040239 vs. TAIR 10
Match: AT5G15860.2 (prenylcysteine methylesterase )

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-83
Identity = 148/218 (67.89%), Postives = 177/218 (81.19%), Query Frame = 0

Query: 110 RHQTVDRHVENADVVRAERFLLTRFGLKLSKYIRVAIRYIARFLALGCYSLFLLPGFLQV 169
           R Q+  R + +A    AE +L+T    KL +Y+ V  R++ + LAL CY++ L+PGFLQV
Sbjct: 63  RQQSFGRDIGHA---AAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQV 122

Query: 170 GYYYFSSSQIRRSIPYGDKPRNKLDLYLPKHIDGPKPVVAFITGGAWIIGYKAWGCLLGQ 229
            Y YF S Q+RRSI YGD+PRN+LDLYLP + DG KPVV F+TGGAWIIGYKAWG LLG 
Sbjct: 123 AYSYFFSKQVRRSIVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGSLLGM 182

Query: 230 QLSERDVIVACIDYRNFPQGTMSDMIDDASQGISFLCNNIREFGGDPNRIFLMGQSAGAH 289
           QL+ERD+IVAC+DYRNFPQGT+SDM+ DASQGISF+CNNI  FGGDPNRI+LMGQSAGAH
Sbjct: 183 QLAERDIIVACLDYRNFPQGTISDMVTDASQGISFVCNNISAFGGDPNRIYLMGQSAGAH 242

Query: 290 IAACTLLEQAIKEVRRVESISWSVSQIKAYFGLSGGIM 328
           IAAC LLEQA KE++  ESISW+VSQIKAYFGLSGG +
Sbjct: 243 IAACALLEQATKELKG-ESISWTVSQIKAYFGLSGGYL 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891409.11.2e-22983.47LOW QUALITY PROTEIN: isoprenylcysteine alpha-carbonyl methylesterase ICME [Benin... [more]
KAA0034515.12.3e-22581.27isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 [Cucumis melo va... [more]
XP_022957176.15.0e-22581.27isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 [Cucurbita mosch... [more]
XP_004135123.21.1e-22481.27isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 [Cucumis sativus... [more]
XP_022977831.17.3e-22480.68isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
Q8VYP91.7e-13052.21Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1 OS=Arabidopsis t... [more]
Q94AS55.2e-12457.51Isoprenylcysteine alpha-carbonyl methylesterase ICME OS=Arabidopsis thaliana OX=... [more]
Q1PET61.2e-12056.42Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL2 OS=Arabidopsis t... [more]
Q5Z9I24.6e-11251.95Probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1 OS=Oryza sativa ... [more]
Q6L5F58.9e-10851.25Probable isoprenylcysteine alpha-carbonyl methylesterase ICME OS=Oryza sativa su... [more]
Match NameE-valueIdentityDescription
A0A5D3CFI91.1e-22581.27Isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 OS=Cucumis melo ... [more]
A0A6J1GYH92.4e-22581.27isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 OS=Cucurbita mos... [more]
A0A0A0KUE35.4e-22581.27Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6082... [more]
A0A6J1IL233.5e-22480.68isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X2 OS=Cucurbita max... [more]
A0A6J1GZT46.6e-22381.12isoprenylcysteine alpha-carbonyl methylesterase ICME isoform X1 OS=Cucurbita mos... [more]
Match NameE-valueIdentityDescription
AT1G26120.11.2e-13152.21alpha/beta-Hydrolases superfamily protein [more]
AT5G15860.13.7e-12557.51prenylcysteine methylesterase [more]
AT3G02410.18.5e-12256.42alpha/beta-Hydrolases superfamily protein [more]
AT3G02410.24.8e-11760.11alpha/beta-Hydrolases superfamily protein [more]
AT5G15860.22.7e-8367.89prenylcysteine methylesterase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 173..409
e-value: 1.3E-54
score: 187.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 175..400
IPR002018Carboxylesterase, type BPFAMPF00135COesterasecoord: 193..301
e-value: 1.6E-13
score: 50.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 112..330
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 326..414
NoneNo IPR availablePANTHERPTHR23024:SF516ISOPRENYLCYSTEINE ALPHA-CARBONYL METHYLESTERASE ICMEL1-RELATEDcoord: 326..414
NoneNo IPR availablePANTHERPTHR23024:SF516ISOPRENYLCYSTEINE ALPHA-CARBONYL METHYLESTERASE ICMEL1-RELATEDcoord: 112..330
IPR019826Carboxylesterase type B, active sitePROSITEPS00122CARBOXYLESTERASE_B_1coord: 272..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040239.1Lag0040239.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010296 prenylcysteine methylesterase activity