Lag0040185 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040185
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cytochrome P450 CYP749A22-like
Location	chr13: 2769535 .. 2769959 (-)
RNA-Seq Expression	Lag0040185
Synteny	Lag0040185

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGCTCCATAATTTCAATAATAAAAAACAAAGAAGAAAATGTGATACGTGGTGAAGTAGATAACTATGGTAATGATTTTCTTGGATTGCTTTTGAAGGCAAAGAATGACCCTGACCAGACACAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATATACTTTGCGGGGCAAGAAACTACAAATGTTCTGATTGCTTGGATTGCTTTTGAGGTTTGAGTAAATTAAACCTTTGAATCAATTCATCCAATGTATCTGTTGCACTAAATTTAGTGTTAGTATTGTAGTTTGAGTTGTGTTTGTAAATACAGCTGGCTATACAGTTGAATAAACCAGACAAGACAACAAACTTATCTGTATATGTGATTGTAATCTTTCCTTCTCCTGTGAATAAAAAAGAATCCTGCTCCTAG

mRNA sequence

ATGAAAAGCTCCATAATTTCAATAATAAAAAACAAAGAAGAAAATGTGATACGTGGTGAAGTAGATAACTATGGTAATGATTTTCTTGGATTGCTTTTGAAGGCAAAGAATGACCCTGACCAGACACAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATATACTTTGCGGGGCAAGAAACTACAAATGTTCTGATTGCTTGGATTGCTTTTGAGCTGGCTATACAGTTGAATAAACCAGACAAGACAACAAACTTATCTGTATATGTGATTGTAATCTTTCCTTCTCCTGTGAATAAAAAAGAATCCTGCTCCTAG

Coding sequence (CDS)

ATGAAAAGCTCCATAATTTCAATAATAAAAAACAAAGAAGAAAATGTGATACGTGGTGAAGTAGATAACTATGGTAATGATTTTCTTGGATTGCTTTTGAAGGCAAAGAATGACCCTGACCAGACACAGAGGATTTCTGTTGAGGATGTGGTAGATGAATGCAAAGCAATATACTTTGCGGGGCAAGAAACTACAAATGTTCTGATTGCTTGGATTGCTTTTGAGCTGGCTATACAGTTGAATAAACCAGACAAGACAACAAACTTATCTGTATATGTGATTGTAATCTTTCCTTCTCCTGTGAATAAAAAAGAATCCTGCTCCTAG

Protein sequence

MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFAGQETTNVLIAWIAFELAIQLNKPDKTTNLSVYVIVIFPSPVNKKESCS

Homology

[Close]

BLAST of Lag0040185 vs. NCBI nr
Match: XP_022149008.1 (cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 127.5 bits (319), Expect = 7.0e-26
Identity = 59/81 (72.84%), Postives = 70/81 (86.42%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           MK+SI+SIIK +EE V+ GE DNYGNDFLGLLLKAKNDPD  QRISV+DVVDECK +YFA
Sbjct: 262 MKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDPDPRQRISVDDVVDECKTVYFA 321

Query: 61  GQETTNVLIAWIAFELAIQLN 82
           GQETTNVL++W +F LA  ++
Sbjct: 322 GQETTNVLLSWTSFLLATHVD 342

[Close]

BLAST of Lag0040185 vs. NCBI nr
Match: XP_022149052.1 (cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 114.0 bits (284), Expect = 8.1e-22
Identity = 51/85 (60.00%), Postives = 65/85 (76.47%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           MK   + +IK++EE  I GE D YGNDFLGLL+KAKNDP+++QRISV+D+VDECK  YFA
Sbjct: 263 MKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVDECKTFYFA 322

Query: 61  GQETTNVLIAWIAFELAIQLNKPDK 86
           G ETT+VL+AW  F LA+     D+
Sbjct: 323 GHETTSVLLAWTMFLLALHKQWQDQ 347

[Close]

BLAST of Lag0040185 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-21
Identity = 53/88 (60.23%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           MK   + IIK +EE +  GE D YGNDFLGLL+KAKNDP+ +QRISVED+VDECK  YFA
Sbjct: 263 MKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKNDPEISQRISVEDIVDECKTFYFA 322

Query: 61  GQETTNVLIAWIAFELAIQLNKPDKTTN 89
           G ETTNVL+AW  F LA+     ++  N
Sbjct: 323 GHETTNVLLAWTMFLLALHKEWQEEARN 350

[Close]

BLAST of Lag0040185 vs. NCBI nr
Match: XP_038892926.1 (cytochrome P450 CYP749A22-like isoform X2 [Benincasa hispida])

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-21
Identity = 53/88 (60.23%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           MK   + IIK +EE +  GE D YGNDFLGLL+KAKNDP+ +QRISVED+VDECK  YFA
Sbjct: 231 MKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKNDPEISQRISVEDIVDECKTFYFA 290

Query: 61  GQETTNVLIAWIAFELAIQLNKPDKTTN 89
           G ETTNVL+AW  F LA+     ++  N
Sbjct: 291 GHETTNVLLAWTMFLLALHKEWQEEARN 318

[Close]

BLAST of Lag0040185 vs. NCBI nr
Match: XP_004135429.2 (cytochrome P450 CYP749A22 [Cucumis sativus] >KAE8648771.1 hypothetical protein Csa_009204 [Cucumis sativus])

HSP 1 Score: 110.5 bits (275), Expect = 8.9e-21
Identity = 52/88 (59.09%), Postives = 63/88 (71.59%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           MK   + IIK +EE +   E ++YGNDFLGLL+KAKNDP  +QRIS+EDVVDECK  YFA
Sbjct: 265 MKDCFVEIIKAREEKLNNDEANDYGNDFLGLLVKAKNDPQDSQRISLEDVVDECKTFYFA 324

Query: 61  GQETTNVLIAWIAFELAIQLNKPDKTTN 89
           G ETTNVL+AW  F LA+     +K  N
Sbjct: 325 GHETTNVLLAWTMFLLALHKEWQEKARN 352

[Close]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 7.3e-18
Identity = 43/83 (51.81%), Postives = 59/83 (71.08%), Query Frame = 0


Query: 4   SIISIIKNKE-ENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFAGQ 63
           SI+ I++ +E E+ + GEV ++G DFLGLL+KA ND D+  RI+ +DVVDECK  Y AGQ
Sbjct: 276 SILRIMEKREKESTMSGEVGSFGTDFLGLLMKAMNDADEKNRITAQDVVDECKTFYVAGQ 335

Query: 64  ETTNVLIAWIAFELAIQLNKPDK 86
           ETT  L+AW+ F L I  +  +K
Sbjct: 336 ETTTTLLAWVIFLLGIHTDWQEK 358

[Close]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 4.8e-09
Identity = 29/85 (34.12%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFA 60
           M +SI  II ++ ++       +YG+D LG+LLKA N   + +++S+E+++ EC+  +F 
Sbjct: 271 MDNSIKRIISSRLQS-----KSDYGDDLLGILLKAYNTEGKERKMSIEEIIHECRTFFFG 330

Query: 61  GQETTNVLIAWIAFELAIQLNKPDK 86
           G ETT+ L+AW    L++  +  +K
Sbjct: 331 GHETTSNLLAWTTMLLSLHQDWQEK 350

[Close]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.1e-09
Identity = 26/79 (32.91%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 7   SIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFAGQETTN 66
           S IK   +  +  E  +YGND LG++L A +  +  +++S++++++ECK  +FAG ETT 
Sbjct: 326 SSIKRIIDARLTSESKDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFFAGHETTA 385

Query: 67  VLIAWIAFELAIQLNKPDK 86
            L+ W    L++  +  +K
Sbjct: 386 NLLTWSTMLLSLHQDWQEK 404

[Close]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.3e-08
Identity = 27/82 (32.93%), Postives = 52/82 (63.41%), Query Frame = 0


Query: 1   MKSSIISIIKNKEENVIRGEVDN----YGNDFLGLLLKAKNDPDQTQRISVEDVVDECKA 60
           ++ S++ +I+ + +N I GE +        D LGL+++AKN       ++V+D+V+ECK+
Sbjct: 268 IRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAKN-------VTVQDIVEECKS 327

Query: 61  IYFAGQETTNVLIAWIAFELAI 79
            +FAG++TT+ L+ W    L++
Sbjct: 328 FFFAGKQTTSNLLTWTTILLSM 342

[Close]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-07
Identity = 26/73 (35.62%), Postives = 45/73 (61.64%), Query Frame = 0


Query: 5   IISIIKNKEENVIRGEVDNYGNDFLGLLLKAKNDPDQTQRISVEDVVDECKAIYFAGQET 64
           ++ II +++++V  G   +YG+D LGLLL   +       ++V+ ++DECK  +F G ET
Sbjct: 269 LMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMD--SNKNNLNVQMIMDECKTFFFTGHET 328

Query: 65  TNVLIAWIAFELA 78
           T++L+ W    LA
Sbjct: 329 TSLLLTWTLMLLA 339

The following BLAST results are available for this feature:

BLAST of Lag0040185 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149008.1	7.0e-26	72.84	cytochrome P450 CYP749A22-like [Momordica charantia]	[more]
XP_022149052.1	8.1e-22	60.00	cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome ...	[more]
XP_038892924.1	1.1e-21	60.23	cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida]	[more]
XP_038892926.1	1.1e-21	60.23	cytochrome P450 CYP749A22-like isoform X2 [Benincasa hispida]	[more]
XP_004135429.2	8.9e-21	59.09	cytochrome P450 CYP749A22 [Cucumis sativus] >KAE8648771.1 hypothetical protein C...	[more]

BLAST of Lag0040185 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH17	7.3e-18	51.81	Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
Q9ASR3	4.8e-09	34.12	Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1	[more]
F4IK45	8.1e-09	32.91	Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1	[more]
O48786	5.3e-08	32.93	Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1	[more]
Q9ZW95	1.5e-07	35.62	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..77 e-value: 2.0E-6 score: 26.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..85 e-value: 2.4E-12 score: 48.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 23..78
None	No IPR available	PANTHER	PTHR24282:SF219	CYTOCHROME P450 CYP749A22-LIKE ISOFORM X1	coord: 3..88
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 3..88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040185.1	Lag0040185.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen