Lag0040087 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040087
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr13: 2068039 .. 2069883 (-)
RNA-Seq ExpressionLag0040087
SyntenyLag0040087
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCGCGAAATAGCGCGGTTGGTGGCAGATGGGTTTTACATCGAAGCACTCTCCATGTACTCACAGCACCACTCGGCCTCCCTTCGTCCTCACAATTTCATTTTCCCTCCTCTCTTCAAAGCATGCGCAAAGCTTAACTCAGTTCCACAAGGCAAAATGCTACACACCCACTTGATCAAAACAGGGTTTCTTGCAGATGTCTATGCAGCAACAGCCCTCACAGATATGTACGTGAAACTTCTTCATTTTGAAGATGCTCTAAAGGTGTTCGACGAAATGCCTCACAGAAACTTGGCATCTTTAAATACAATGATTTCTGGGTTTTCGATGAATGGTTCACGTGGAGAAGCATTACGGTTGGCCGAGCTTGTGAATCGTGGTTCACTAAGGCCTAATACAATTACCATTGTTAACTTGCTGTCAGCATGCGCAAGTGTTGCTCATGGAATGCAAATACATTGTTGGGCTATAAAACTGGGTTTTCAAATGGATGTCTATGTTGCTACGGCGCTCTTGACGATGTATTCTACTTGTGAAGAAGTGGGTTGCGCTGCTAAGGTATTTCAGGATATGTCGAACAAAAATGTGGTGAGTTTTAATGCTTATATTTCAGGGCTTCTACTGAATGACATGCCTCGTACGGTGTTGGATATATTTAAGAACATGATGGAATGCCCATGTGCGAAACCAAATTCAGTCACACTGGCTTCTGTCCTTTCTGCTTGTTCCAATCTTTCCCACCTTGGATTTGGTAGGCAGATTCATGCACTCACTATCAAAATTGATAGCGATGATGTAATAGTAGGAACTGCACTGGTGGACATGTATTCTAAATGTGGGGCTTGGCAGTGGGCACATAATCTTTTCAACGAGCGGATAGAAACCAATAACTTAATTACTTGGAATTCATTGATTGCTGGAATGATGTTAAATGATCAGAGTGAGATTGCAATGGAACTTTTTGAATCTTTGGAGTCCCACAAATTGCAACCAGATTCAGCTACGTGGAATTCAATGATTAGTGGACTTGCCCGATCGGGTCGGCCAATTGAGGCCTTCAAGTACTTTCATAGAATGCAATCGGCTGGGATAGTCCCGAGTTTAAAATCTCTCACCAGTCTCTTATCCATTTGTTCAAATTTGTCTGCATTGCGGCATGGCAAAGAGATTCATACACAAGCAATTAGAAGCATCATTCATATGGATGTGTTTCTTGCCACGGCACTAATTGACATGTACATGAAGTGTGGACATCTTTCTTCAGCACGAAAACTCTTCGATCAATTTGACTTCAAACCAAAAGATACAATATTTTGGAACGCAATTATCTCAGGTTACGGAAGAAATGGAGAGAACAAATCTGCATTTGATATCTTTGATCGAATGTTGGAAGAAAAAGTACAACCAAATGTGGCCACGTTTACCAGTCTCTTATCTATTTGCAGTCATTGTGGTCAGGTTGACAAAGGTTGGCAATTTTTCAGAATGATGAACAAGAAATATGATCTGCAGCCATATCGTGACCATTATAGCTGCATGATAGACATTTTGAGTAGAGCCGGCCAGTTGGGAAAAGCTAGAGAATTGTTGGAAGAATTGCCAGAGCCTTCTATGTCTTCTCTTGCTTCTTTGCTTGGTGCTTGTAGCTTGCACAAAGATCCTAAACTGGGCGAAGAAATGGCATTAAGAATTTCAGAATTAGAGCCGAAGAACCCACTTCCATTTGTTATATTATCCAATATATATGCTGAGCTTGAAAGGTGGAAAGATGTTGAAAGGGTTAGAGAAATGATGAATGATAAAGGATTGAGAAAGCCATCCGGCATTAGTTCAATAGAATGA

mRNA sequence

ATGAAGCGCGAAATAGCGCGGTTGGTGGCAGATGGGTTTTACATCGAAGCACTCTCCATGTACTCACAGCACCACTCGGCCTCCCTTCGTCCTCACAATTTCATTTTCCCTCCTCTCTTCAAAGCATGCGCAAAGCTTAACTCAGTTCCACAAGGCAAAATGCTACACACCCACTTGATCAAAACAGGGTTTCTTGCAGATGTCTATGCAGCAACAGCCCTCACAGATATGTACGTGAAACTTCTTCATTTTGAAGATGCTCTAAAGGTGTTCGACGAAATGCCTCACAGAAACTTGGCATCTTTAAATACAATGATTTCTGGGTTTTCGATGAATGGTTCACGTGGAGAAGCATTACGGTTGGCCGAGCTTGTGAATCGTGGTTCACTAAGGCCTAATACAATTACCATTGTTAACTTGCTGTCAGCATGCGCAAGTGTTGCTCATGGAATGCAAATACATTGTTGGGCTATAAAACTGGGTTTTCAAATGGATGTCTATGTTGCTACGGCGCTCTTGACGATGTATTCTACTTGTGAAGAAGTGGGTTGCGCTGCTAAGGTATTTCAGGATATGTCGAACAAAAATGTGGTGAGTTTTAATGCTTATATTTCAGGGCTTCTACTGAATGACATGCCTCGTACGGTGTTGGATATATTTAAGAACATGATGGAATGCCCATGTGCGAAACCAAATTCAGTCACACTGGCTTCTGTCCTTTCTGCTTGTTCCAATCTTTCCCACCTTGGATTTGGTAGGCAGATTCATGCACTCACTATCAAAATTGATAGCGATGATGTAATAGTAGGAACTGCACTGGTGGACATGTATTCTAAATGTGGGGCTTGGCAGTGGGCACATAATCTTTTCAACGAGCGGATAGAAACCAATAACTTAATTACTTGGAATTCATTGATTGCTGGAATGATGTTAAATGATCAGAGTGAGATTGCAATGGAACTTTTTGAATCTTTGGAGTCCCACAAATTGCAACCAGATTCAGCTACGTGGAATTCAATGATTAGTGGACTTGCCCGATCGGGTCGGCCAATTGAGGCCTTCAAGTACTTTCATAGAATGCAATCGGCTGGGATAGTCCCGAGTTTAAAATCTCTCACCAGTCTCTTATCCATTTGTTCAAATTTGTCTGCATTGCGGCATGGCAAAGAGATTCATACACAAGCAATTAGAAGCATCATTCATATGGATGTGTTTCTTGCCACGGCACTAATTGACATGTACATGAAGTGTGGACATCTTTCTTCAGCACGAAAACTCTTCGATCAATTTGACTTCAAACCAAAAGATACAATATTTTGGAACGCAATTATCTCAGGTTACGGAAGAAATGGAGAGAACAAATCTGCATTTGATATCTTTGATCGAATGTTGGAAGAAAAAGTACAACCAAATGTGGCCACGTTTACCAGTCTCTTATCTATTTGCAGTCATTGTGGTCAGGTTGACAAAGGTTGGCAATTTTTCAGAATGATGAACAAGAAATATGATCTGCAGCCATATCGTGACCATTATAGCTGCATGATAGACATTTTGAGTAGAGCCGGCCAGTTGGGAAAAGCTAGAGAATTGTTGGAAGAATTGCCAGAGCCTTCTATGTCTTCTCTTGCTTCTTTGCTTGGTGCTTGTAGCTTGCACAAAGATCCTAAACTGGGCGAAGAAATGGCATTAAGAATTTCAGAATTAGAGCCGAAGAACCCACTTCCATTTGTTATATTATCCAATATATATGCTGAGCTTGAAAGGTGGAAAGATGTTGAAAGGGTTAGAGAAATGATGAATGATAAAGGATTGAGAAAGCCATCCGGCATTAGTTCAATAGAATGA

Coding sequence (CDS)

ATGAAGCGCGAAATAGCGCGGTTGGTGGCAGATGGGTTTTACATCGAAGCACTCTCCATGTACTCACAGCACCACTCGGCCTCCCTTCGTCCTCACAATTTCATTTTCCCTCCTCTCTTCAAAGCATGCGCAAAGCTTAACTCAGTTCCACAAGGCAAAATGCTACACACCCACTTGATCAAAACAGGGTTTCTTGCAGATGTCTATGCAGCAACAGCCCTCACAGATATGTACGTGAAACTTCTTCATTTTGAAGATGCTCTAAAGGTGTTCGACGAAATGCCTCACAGAAACTTGGCATCTTTAAATACAATGATTTCTGGGTTTTCGATGAATGGTTCACGTGGAGAAGCATTACGGTTGGCCGAGCTTGTGAATCGTGGTTCACTAAGGCCTAATACAATTACCATTGTTAACTTGCTGTCAGCATGCGCAAGTGTTGCTCATGGAATGCAAATACATTGTTGGGCTATAAAACTGGGTTTTCAAATGGATGTCTATGTTGCTACGGCGCTCTTGACGATGTATTCTACTTGTGAAGAAGTGGGTTGCGCTGCTAAGGTATTTCAGGATATGTCGAACAAAAATGTGGTGAGTTTTAATGCTTATATTTCAGGGCTTCTACTGAATGACATGCCTCGTACGGTGTTGGATATATTTAAGAACATGATGGAATGCCCATGTGCGAAACCAAATTCAGTCACACTGGCTTCTGTCCTTTCTGCTTGTTCCAATCTTTCCCACCTTGGATTTGGTAGGCAGATTCATGCACTCACTATCAAAATTGATAGCGATGATGTAATAGTAGGAACTGCACTGGTGGACATGTATTCTAAATGTGGGGCTTGGCAGTGGGCACATAATCTTTTCAACGAGCGGATAGAAACCAATAACTTAATTACTTGGAATTCATTGATTGCTGGAATGATGTTAAATGATCAGAGTGAGATTGCAATGGAACTTTTTGAATCTTTGGAGTCCCACAAATTGCAACCAGATTCAGCTACGTGGAATTCAATGATTAGTGGACTTGCCCGATCGGGTCGGCCAATTGAGGCCTTCAAGTACTTTCATAGAATGCAATCGGCTGGGATAGTCCCGAGTTTAAAATCTCTCACCAGTCTCTTATCCATTTGTTCAAATTTGTCTGCATTGCGGCATGGCAAAGAGATTCATACACAAGCAATTAGAAGCATCATTCATATGGATGTGTTTCTTGCCACGGCACTAATTGACATGTACATGAAGTGTGGACATCTTTCTTCAGCACGAAAACTCTTCGATCAATTTGACTTCAAACCAAAAGATACAATATTTTGGAACGCAATTATCTCAGGTTACGGAAGAAATGGAGAGAACAAATCTGCATTTGATATCTTTGATCGAATGTTGGAAGAAAAAGTACAACCAAATGTGGCCACGTTTACCAGTCTCTTATCTATTTGCAGTCATTGTGGTCAGGTTGACAAAGGTTGGCAATTTTTCAGAATGATGAACAAGAAATATGATCTGCAGCCATATCGTGACCATTATAGCTGCATGATAGACATTTTGAGTAGAGCCGGCCAGTTGGGAAAAGCTAGAGAATTGTTGGAAGAATTGCCAGAGCCTTCTATGTCTTCTCTTGCTTCTTTGCTTGGTGCTTGTAGCTTGCACAAAGATCCTAAACTGGGCGAAGAAATGGCATTAAGAATTTCAGAATTAGAGCCGAAGAACCCACTTCCATTTGTTATATTATCCAATATATATGCTGAGCTTGAAAGGTGGAAAGATGTTGAAAGGGTTAGAGAAATGATGAATGATAAAGGATTGAGAAAGCCATCCGGCATTAGTTCAATAGAATGA

Protein sequence

MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRMQSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMSSLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKGLRKPSGISSIE
Homology
BLAST of Lag0040087 vs. NCBI nr
Match: KAG7032196.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1072.8 bits (2773), Expect = 9.4e-310
Identity = 527/614 (85.83%), Postives = 566/614 (92.18%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  +NFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSYNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MPR V+D+FK+MMECP  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPRMVIDVFKSMMECPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AH +FNER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHKVFNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG AR G+P++AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHARLGQPVKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTSLLSICS+LSALRHGKEIH Q  +S IHMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSLLSICSDLSALRHGKEIHAQVTKSFIHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQFF+MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFFKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLH+D KLGEEMA RISELEPKNPLPFVILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHRDSKLGEEMASRISELEPKNPLPFVILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. NCBI nr
Match: XP_023537719.1 (pentatricopeptide repeat-containing protein At2g02750 [Cucurbita pepo subsp. pepo] >XP_023537727.1 pentatricopeptide repeat-containing protein At2g02750 [Cucurbita pepo subsp. pepo] >XP_023537736.1 pentatricopeptide repeat-containing protein At2g02750 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.4e-309
Identity = 528/614 (85.99%), Postives = 564/614 (91.86%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  HNFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSHNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGFS+NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFSLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNR SL+PN+ITIVNLLSACASVAHG+QIHCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRDSLKPNSITIVNLLSACASVAHGIQIHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MPR V+D+FK+MMECP  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPRMVIDVFKSMMECPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AH +FNER  T+NL 
Sbjct: 241 SACSTLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHKVFNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG A  G+P++AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHAHLGQPVKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTSLLSICS+LSALRHGKEIH Q  +S IHMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSLLSICSDLSALRHGKEIHAQVTKSFIHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQFF+MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFFKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPFVILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFVILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. NCBI nr
Match: KAG6601415.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1070.1 bits (2766), Expect = 7.1e-309
Identity = 527/614 (85.83%), Postives = 565/614 (92.02%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  +NFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSYNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MPR V+D+FK+MMECP  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPRMVIDVFKSMMECPPGKSNSVTLFSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ A  +FNER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCARKVFNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG AR G+P++AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHARLGQPVKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTSLLSICS+LSALRHGKEIH Q  +S IHMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSLLSICSDLSALRHGKEIHAQVTKSFIHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQFF+MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFFKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPFVILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFVILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. NCBI nr
Match: XP_022998231.1 (pentatricopeptide repeat-containing protein At2g02750 [Cucurbita maxima])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.0e-307
Identity = 525/614 (85.50%), Postives = 561/614 (91.37%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  HNFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSHNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MPR V+D+FK+MME P  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPRMVIDVFKSMMERPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AHN+ NER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHNVLNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG A  G+P +AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHAHLGQPAKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTS LSICS+LSALRHGKEIH Q  +  IHMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSFLSICSDLSALRHGKEIHAQVTKRFIHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQFF+MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFFKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPFVILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFVILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. NCBI nr
Match: XP_022956404.1 (pentatricopeptide repeat-containing protein At2g02750 [Cucurbita moschata])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.2e-307
Identity = 522/614 (85.02%), Postives = 562/614 (91.53%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  +NFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSYNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MP  V+D+FK+MMECP  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPPMVIDVFKSMMECPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AH +FNER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHKVFNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG AR G+P++AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHARLGQPVKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTSLL +CS+LSALRHGKEIH Q  +S  HMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSLLFVCSDLSALRHGKEIHAQVTKSFTHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQF +MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFLKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPF+ILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFIILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. ExPASy Swiss-Prot
Match: Q1PFA6 (Pentatricopeptide repeat-containing protein At2g02750 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E22 PE=2 SV=2)

HSP 1 Score: 624.0 bits (1608), Expect = 1.8e-177
Identity = 301/608 (49.51%), Postives = 435/608 (71.55%), Query Frame = 0

Query: 5   IARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGF 64
           ++ LV  G  ++ +  +S        P+ F FPPL K+CAKL  V QG++LH  ++KTGF
Sbjct: 11  VSNLVTGGTSLDVILSHS--------PNKFTFPPLLKSCAKLGDVVQGRILHAQVVKTGF 70

Query: 65  LADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAEL 124
             DV+ ATAL  MY+K+    DALKV DEMP R +AS+N  +SG   NG   +A R+   
Sbjct: 71  FVDVFTATALVSMYMKVKQVTDALKVLDEMPERGIASVNAAVSGLLENGFCRDAFRMFGD 130

Query: 125 VNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGC 184
                   N++T+ ++L  C  +  GMQ+HC A+K GF+M+VYV T+L++MYS C E   
Sbjct: 131 ARVSGSGMNSVTVASVLGGCGDIEGGMQLHCLAMKSGFEMEVYVGTSLVSMYSRCGEWVL 190

Query: 185 AAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACS 244
           AA++F+ + +K+VV++NA+ISGL+ N +   V  +F  M +    +PN VT  + ++AC+
Sbjct: 191 AARMFEKVPHKSVVTYNAFISGLMENGVMNLVPSVFNLMRKFSSEEPNDVTFVNAITACA 250

Query: 245 NLSHLGFGRQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWN 304
           +L +L +GRQ+H L +K +   + +VGTAL+DMYSKC  W+ A+ +F E  +T NLI+WN
Sbjct: 251 SLLNLQYGRQLHGLVMKKEFQFETMVGTALIDMYSKCRCWKSAYIVFTELKDTRNLISWN 310

Query: 305 SLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRMQSA 364
           S+I+GMM+N Q E A+ELFE L+S  L+PDSATWNS+ISG ++ G+ IEAFK+F RM S 
Sbjct: 311 SVISGMMINGQHETAVELFEKLDSEGLKPDSATWNSLISGFSQLGKVIEAFKFFERMLSV 370

Query: 365 GIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSA 424
            +VPSLK LTSLLS CS++  L++GKEIH   I++    D+F+ T+LIDMYMKCG  S A
Sbjct: 371 VMVPSLKCLTSLLSACSDIWTLKNGKEIHGHVIKAAAERDIFVLTSLIDMYMKCGLSSWA 430

Query: 425 RKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICS 484
           R++FD+F+ KPKD +FWN +ISGYG++GE +SA +IF+ + EEKV+P++ATFT++LS CS
Sbjct: 431 RRIFDRFEPKPKDPVFWNVMISGYGKHGECESAIEIFELLREEKVEPSLATFTAVLSACS 490

Query: 485 HCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMSSLA 544
           HCG V+KG Q FR+M ++Y  +P  +H  CMID+L R+G+L +A+E+++++ EPS S  +
Sbjct: 491 HCGNVEKGSQIFRLMQEEYGYKPSTEHIGCMIDLLGRSGRLREAKEVIDQMSEPSSSVYS 550

Query: 545 SLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKG 604
           SLLG+C  H DP LGEE A++++ELEP+NP PFVILS+IYA LERW+DVE +R++++ K 
Sbjct: 551 SLLGSCRQHLDPVLGEEAAMKLAELEPENPAPFVILSSIYAALERWEDVESIRQVIDQKQ 610

Query: 605 LRKPSGIS 612
           L K  G+S
Sbjct: 611 LVKLPGLS 610

BLAST of Lag0040087 vs. ExPASy Swiss-Prot
Match: Q3E6Q1 (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.5e-102
Identity = 212/604 (35.10%), Postives = 338/604 (55.96%), Query Frame = 0

Query: 16  EALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGFLADVYAATALT 75
           +AL  + +     + P  + F  L K C     +  GK +H  L+K+GF  D++A T L 
Sbjct: 118 KALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLE 177

Query: 76  DMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAELVNRGSLRPNTI 135
           +MY K     +A KVFD MP R+L S NT+++G+S NG    AL + + +   +L+P+ I
Sbjct: 178 NMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFI 237

Query: 136 TIVNLL---SACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGCAAKVFQDM 195
           TIV++L   SA   ++ G +IH +A++ GF   V ++TAL+ MY+ C  +  A ++F  M
Sbjct: 238 TIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGM 297

Query: 196 SNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACSNLSHLGFG 255
             +NVVS+N+ I   + N+ P+  + IF+ M++    KP  V++   L AC++L  L  G
Sbjct: 298 LERNVVSWNSMIDAYVQNENPKEAMLIFQKMLD-EGVKPTDVSVMGALHACADLGDLERG 357

Query: 256 RQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWNSLIAGMML 315
           R IH L++++  D +V V  +L+ MY KC                               
Sbjct: 358 RFIHKLSVELGLDRNVSVVNSLISMYCKC------------------------------- 417

Query: 316 NDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRMQSAGIVPSLKS 375
             + + A  +F  L+S  L     +WN+MI G A++GRPI+A  YF +M+S  + P   +
Sbjct: 418 -KEVDTAASMFGKLQSRTL----VSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFT 477

Query: 376 LTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSARKLFDQFD 435
             S+++  + LS   H K IH   +RS +  +VF+ TAL+DMY KCG +  AR +FD   
Sbjct: 478 YVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMS 537

Query: 436 FKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICSHCGQVDKG 495
            +   T  WNA+I GYG +G  K+A ++F+ M +  ++PN  TF S++S CSH G V+ G
Sbjct: 538 ERHVTT--WNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAG 597

Query: 496 WQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELP-EPSMSSLASLLGACS 555
            + F MM + Y ++   DHY  M+D+L RAG+L +A + + ++P +P+++   ++LGAC 
Sbjct: 598 LKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQ 657

Query: 556 LHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKGLRKPSGI 615
           +HK+    E+ A R+ EL P +    V+L+NIY     W+ V +VR  M  +GLRK  G 
Sbjct: 658 IHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGC 682

BLAST of Lag0040087 vs. ExPASy Swiss-Prot
Match: O04659 (Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E14 PE=2 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 8.8e-100
Identity = 210/591 (35.53%), Postives = 325/591 (54.99%), Query Frame = 0

Query: 31  PHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGFLADVYAATALTDMYVKLLHFEDALKV 90
           P +F FP + KA   L     G+M+HT ++K+G++ DV  A++L  MY K   FE++L+V
Sbjct: 105 PDSFTFPNVIKAYGALGREFLGRMIHTLVVKSGYVCDVVVASSLVGMYAKFNLFENSLQV 164

Query: 91  FDEMPHRNLASLNTMISGFSMNGSRGEALRLAELVNRGSLRPNTITIVNLLSACAS---V 150
           FDEMP R++AS NT+IS F  +G   +AL L   +      PN++++   +SAC+    +
Sbjct: 165 FDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGFEPNSVSLTVAISACSRLLWL 224

Query: 151 AHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGCAAKVFQDMSNKNVVSFNAYISGL 210
             G +IH   +K GF++D YV +AL+ MY  C+ +  A +VFQ M  K++V++N+ I G 
Sbjct: 225 ERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGY 284

Query: 211 LLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACSNLSHLGFGRQIHALTIK-IDSDD 270
           +     ++ ++I  N M     +P+  TL S+L ACS   +L  G+ IH   I+ + + D
Sbjct: 285 VAKGDSKSCVEIL-NRMIIEGTRPSQTTLTSILMACSRSRNLLHGKFIHGYVIRSVVNAD 344

Query: 271 VIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWNSLIAGMMLNDQSEIAMELFESLE 330
           + V  +L+D+Y KCG    A  +F+                                   
Sbjct: 345 IYVNCSLIDLYFKCGEANLAETVFS----------------------------------- 404

Query: 331 SHKLQPDSA-TWNSMISGLARSGRPIEAFKYFHRMQSAGIVPSLKSLTSLLSICSNLSAL 390
             K Q D A +WN MIS     G   +A + + +M S G+ P + + TS+L  CS L+AL
Sbjct: 405 --KTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAAL 464

Query: 391 RHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSARKLFDQFDFKPKDTIFWNAIIS 450
             GK+IH     S +  D  L +AL+DMY KCG+   A ++F+      KD + W  +IS
Sbjct: 465 EKGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFN--SIPKKDVVSWTVMIS 524

Query: 451 GYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICSHCGQVDKGWQFFRMMNKKYDLQ 510
            YG +G+ + A   FD M +  ++P+  T  ++LS C H G +D+G +FF  M  KY ++
Sbjct: 525 AYGSHGQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGLKFFSQMRSKYGIE 584

Query: 511 PYRDHYSCMIDILSRAGQLGKARELLEELPEPSMSS--LASLLGACSLHKDPKLGEEMAL 570
           P  +HYSCMIDIL RAG+L +A E++++ PE S ++  L++L  AC LH +  LG+ +A 
Sbjct: 585 PIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNAELLSTLFSACCLHLEHSLGDRIAR 644

Query: 571 RISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKGLRKPSGISSIE 615
            + E  P +   +++L N+YA  E W    RVR  M + GLRK  G S IE
Sbjct: 645 LLVENYPDDASTYMVLFNLYASGESWDAARRVRLKMKEMGLRKKPGCSWIE 655

BLAST of Lag0040087 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.7e-95
Identity = 204/623 (32.74%), Postives = 338/623 (54.25%), Query Frame = 0

Query: 36  FPPLFKACAKLN-SVPQGKMLHTHLIKTGFLADVYAATALTDMYVKLLHFEDALKVFDEM 95
           F  L  +C K   S    + +H  +IK+GF  +++    L D Y K    ED  +VFD+M
Sbjct: 22  FAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKM 81

Query: 96  PHRNL-------------------------------ASLNTMISGFSMNGSRGEALRLAE 155
           P RN+                                + N+M+SGF+ +    EAL    
Sbjct: 82  PQRNIYTWNSVVTGLTKLGFLDEADSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFA 141

Query: 156 LVNRGSLRPNTITIVNLLSACA---SVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 215
           ++++     N  +  ++LSAC+    +  G+Q+H    K  F  DVY+ +AL+ MYS C 
Sbjct: 142 MMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCG 201

Query: 216 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 275
            V  A +VF +M ++NVVS+N+ I+    N      LD+F+ M+E    +P+ VTLASV+
Sbjct: 202 NVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESR-VEPDEVTLASVI 261

Query: 276 SACSNLSHLGFGRQIHALTIKIDS--DDVIVGTALVDMYSKCGAWQWAHNLFNERIETNN 335
           SAC++LS +  G+++H   +K D   +D+I+  A VDMY+KC   + A  +F + +   N
Sbjct: 262 SACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIF-DSMPIRN 321

Query: 336 LITWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFH 395
           +I   S+I+G  +   ++ A  +F  +    +     +WN++I+G  ++G   EA   F 
Sbjct: 322 VIAETSMISGYAMAASTKAARLMFTKMAERNV----VSWNALIAGYTQNGENEEALSLFC 381

Query: 396 RMQSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIR------SIIHMDVFLATALID 455
            ++   + P+  S  ++L  C++L+ L  G + H   ++      S    D+F+  +LID
Sbjct: 382 LLKRESVCPTHYSFANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLID 441

Query: 456 MYMKCGHLSSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNV 515
           MY+KCG +     +F +     +D + WNA+I G+ +NG    A ++F  MLE   +P+ 
Sbjct: 442 MYVKCGCVEEGYLVFRK--MMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDH 501

Query: 516 ATFTSLLSICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLE 575
            T   +LS C H G V++G  +F  M + + + P RDHY+CM+D+L RAG L +A+ ++E
Sbjct: 502 ITMIGVLSACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIE 561

Query: 576 ELP-EPSMSSLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKD 615
           E+P +P      SLL AC +H++  LG+ +A ++ E+EP N  P+V+LSN+YAEL +W+D
Sbjct: 562 EMPMQPDSVIWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWED 621

BLAST of Lag0040087 vs. ExPASy Swiss-Prot
Match: Q9FWA6 (Pentatricopeptide repeat-containing protein At3g02330, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E90 PE=2 SV=2)

HSP 1 Score: 347.8 bits (891), Expect = 2.5e-94
Identity = 204/616 (33.12%), Postives = 330/616 (53.57%), Query Frame = 0

Query: 5   IARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGF 64
           IA  V +     AL  + +    +      I+  + ++CA L+ +  G  LH H +K+ F
Sbjct: 253 IAGCVQNNLLSLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDF 312

Query: 65  LADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAEL 124
            AD    TA  DMY K  + +DA  +FD   + N  S N MI+G+S      +AL L   
Sbjct: 313 AADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHR 372

Query: 125 VNRGSLRPNTITIVNLLSACASV---AHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEE 184
           +    L  + I++  +  ACA V   + G+QI+  AIK    +DV VA A + MY  C+ 
Sbjct: 373 LMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQA 432

Query: 185 VGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLS 244
           +  A +VF +M  ++ VS+NA I+    N      L +F +M+     +P+  T  S+L 
Sbjct: 433 LAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSR-IEPDEFTFGSILK 492

Query: 245 ACSNLSHLGFGRQIHALTIKID-SDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 304
           AC+  S LG+G +IH+  +K   + +  VG +L+DMYSKCG  + A  + +   +  N  
Sbjct: 493 ACTGGS-LGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRAN-- 552

Query: 305 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 364
                ++G          ME  E + + +LQ    +WNS+ISG     +  +A   F RM
Sbjct: 553 -----VSG---------TMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRM 612

Query: 365 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 424
              GI P   +  ++L  C+NL++   GK+IH Q I+  +  DV++ + L+DMY KCG L
Sbjct: 613 MEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDL 672

Query: 425 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 484
             +R +F++     +D + WNA+I GY  +G+ + A  +F+RM+ E ++PN  TF S+L 
Sbjct: 673 HDSRLMFEK--SLRRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILR 732

Query: 485 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELP-EPSM 544
            C+H G +DKG ++F MM + Y L P   HYS M+DIL ++G++ +A EL+ E+P E   
Sbjct: 733 ACAHMGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADD 792

Query: 545 SSLASLLGACSLHK-DPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREM 604
               +LLG C++H+ + ++ EE    +  L+P++   + +LSN+YA+   W+ V  +R  
Sbjct: 793 VIWRTLLGVCTIHRNNVEVAEEATAALLRLDPQDSSAYTLLSNVYADAGMWEKVSDLRRN 848

Query: 605 MNDKGLRKPSGISSIE 615
           M    L+K  G S +E
Sbjct: 853 MRGFKLKKEPGCSWVE 848

BLAST of Lag0040087 vs. ExPASy TrEMBL
Match: A0A6J1K9P7 (pentatricopeptide repeat-containing protein At2g02750 OS=Cucurbita maxima OX=3661 GN=LOC111492916 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.5e-307
Identity = 525/614 (85.50%), Postives = 561/614 (91.37%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  HNFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSHNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MPR V+D+FK+MME P  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPRMVIDVFKSMMERPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AHN+ NER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHNVLNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG A  G+P +AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHAHLGQPAKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTS LSICS+LSALRHGKEIH Q  +  IHMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSFLSICSDLSALRHGKEIHAQVTKRFIHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQFF+MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFFKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPFVILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFVILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. ExPASy TrEMBL
Match: A0A6J1GYX9 (pentatricopeptide repeat-containing protein At2g02750 OS=Cucurbita moschata OX=3662 GN=LOC111458155 PE=4 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.5e-307
Identity = 522/614 (85.02%), Postives = 562/614 (91.53%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           M REI RLVA GFY+EA SMYSQHHSASL  +NFIFPPLFKACAKLNSVPQG+MLHTHL+
Sbjct: 1   MNREIVRLVAKGFYVEAFSMYSQHHSASLSSYNFIFPPLFKACAKLNSVPQGQMLHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           K GF ADVYAATALTDMY+KL H +DALKVFDEMPHRN ASLN MISGF +NG RGEALR
Sbjct: 61  KVGFSADVYAATALTDMYLKLFHVDDALKVFDEMPHRNTASLNAMISGFLLNGFRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + ELVNRGSL+PN+ITIVNLLSACASVAHGMQ+HCWAI LGFQMDVYVATALLTMY TCE
Sbjct: 121 MVELVNRGSLKPNSITIVNLLSACASVAHGMQVHCWAINLGFQMDVYVATALLTMYCTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           ++G AAKVFQ+MSNKNVVSFNAYISGLLLN MP  V+D+FK+MMECP  K NSVTL SVL
Sbjct: 181 KMGFAAKVFQEMSNKNVVSFNAYISGLLLNGMPPMVIDVFKSMMECPPGKSNSVTLVSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LSHLGFGRQ+HALT+KID+DDV+VGTALVDMYSKCGAWQ AH +FNER  T+NL 
Sbjct: 241 SACSSLSHLGFGRQVHALTVKIDNDDVMVGTALVDMYSKCGAWQCAHKVFNERKGTSNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNSLIAGMMLN+QSEIA+ELFESLESHKLQPDSATWNSMISG AR G+P++AFKYFHRM
Sbjct: 301 TWNSLIAGMMLNEQSEIAVELFESLESHKLQPDSATWNSMISGHARLGQPVKAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPSLKSLTSLL +CS+LSALRHGKEIH Q  +S  HMDV LATALIDMYMKCG L
Sbjct: 361 QSAGTVPSLKSLTSLLFVCSDLSALRHGKEIHAQVTKSFTHMDVLLATALIDMYMKCGCL 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           SSA++LFDQF FKPKDTIFWNA+ISGYG NGENKSAFDIFDRMLEE V PN ATFTSLLS
Sbjct: 421 SSAQRLFDQFGFKPKDTIFWNAMISGYGNNGENKSAFDIFDRMLEEGVHPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSHCGQVDKGWQF +MMNKKYDLQP RDHY+CMIDIL RAGQLGKAR+LLEELPEPSMS
Sbjct: 481 ICSHCGQVDKGWQFLKMMNKKYDLQPDRDHYNCMIDILGRAGQLGKARKLLEELPEPSMS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLHKD KLGEEMA RISELEPKNPLPF+ILSNIYAEL RWKDVERVREMMN
Sbjct: 541 SLASLLGACSLHKDSKLGEEMASRISELEPKNPLPFIILSNIYAELGRWKDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRKPSG+SSIE
Sbjct: 601 DKGLRKPSGLSSIE 614

BLAST of Lag0040087 vs. ExPASy TrEMBL
Match: A0A6J1DCC8 (pentatricopeptide repeat-containing protein At2g02750 OS=Momordica charantia OX=3673 GN=LOC111019194 PE=4 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 3.0e-305
Identity = 517/614 (84.20%), Postives = 558/614 (90.88%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           MKREI RLVA G YIEALS+YSQHHSASLRPH F+FPPLFKACAKLN VPQGKMLHTHL+
Sbjct: 1   MKREIVRLVASGLYIEALSIYSQHHSASLRPHKFLFPPLFKACAKLNFVPQGKMLHTHLV 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           KTGF+ADVYAATALTDMY+KLLHF+DALKVFDEMPHRNLASLN MISGF  NGSRGEALR
Sbjct: 61  KTGFIADVYAATALTDMYMKLLHFDDALKVFDEMPHRNLASLNAMISGFLFNGSRGEALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + E+VN GSLRPN+ITIVNLLS C  VAHGMQIHCWA KLGFQ+DVYVATALLTMY TCE
Sbjct: 121 MVEIVNSGSLRPNSITIVNLLSGCERVAHGMQIHCWATKLGFQVDVYVATALLTMYFTCE 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           EVG AAKVFQ+M NKNVVS+NAYISGLLLNDMPR VLD+FK+MMECPC KPNSVTLASVL
Sbjct: 181 EVGFAAKVFQEMWNKNVVSYNAYISGLLLNDMPRKVLDVFKSMMECPCGKPNSVTLASVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 300
           SACS+LS LGFGRQ+HALT++I +DDV+VGTALVDMYSKCGAWQWA+N+F ER  T NL 
Sbjct: 241 SACSDLSDLGFGRQVHALTVRIQNDDVMVGTALVDMYSKCGAWQWAYNVFKERKRTGNLF 300

Query: 301 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 360
           TWNS+IAGMMLN QSEIAMELFE LE  KLQPDSATWNSMISG AR GRP+EAFKYFHRM
Sbjct: 301 TWNSVIAGMMLNGQSEIAMELFELLEFQKLQPDSATWNSMISGFARLGRPVEAFKYFHRM 360

Query: 361 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 420
           QSAG VPS+KSLTSLLSIC++LSALRHGKEIH Q  + +IH+D+FLATALID YMKCG+ 
Sbjct: 361 QSAGAVPSIKSLTSLLSICADLSALRHGKEIHAQTTKRVIHLDMFLATALIDTYMKCGNF 420

Query: 421 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 480
           S AR+LFDQFD KPKDTIFWN +ISGYGRNGEN  AFDIFDRMLEE V+PN ATFTSLLS
Sbjct: 421 SWARRLFDQFDPKPKDTIFWNTMISGYGRNGENTCAFDIFDRMLEENVRPNAATFTSLLS 480

Query: 481 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMS 540
           ICSH GQV+KGWQ FRMMNK+Y LQPYRDH SCMID+L RAGQLGKAR+LLEELPEPS+S
Sbjct: 481 ICSHSGQVEKGWQLFRMMNKQYGLQPYRDHCSCMIDLLGRAGQLGKARKLLEELPEPSIS 540

Query: 541 SLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMN 600
           SLASLLGACSLH D KLGEEMALRISELEPKNPLPF ILSNIYAEL RW+DVERVREMMN
Sbjct: 541 SLASLLGACSLHNDSKLGEEMALRISELEPKNPLPFSILSNIYAELGRWRDVERVREMMN 600

Query: 601 DKGLRKPSGISSIE 615
           DKGLRK SGISSIE
Sbjct: 601 DKGLRKLSGISSIE 614

BLAST of Lag0040087 vs. ExPASy TrEMBL
Match: A0A314YQJ5 (Pentatricopeptide repeat-containing protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_14577 PE=4 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 6.3e-226
Identity = 392/612 (64.05%), Postives = 479/612 (78.27%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           MKREIARLVADG Y +AL +Y+Q HSASLRPH F FPPL KAC KL S PQ ++LHTHL+
Sbjct: 1   MKREIARLVADGLYRDALCLYAQLHSASLRPHKFTFPPLLKACGKLQSAPQAQILHTHLM 60

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           KTGF ADVY+ATALTD+Y+KL   +DA+KVF+EMP RNLASLN +I+GF  NG   EALR
Sbjct: 61  KTGFSADVYSATALTDVYMKLHLIDDAVKVFEEMPERNLASLNAVITGFLQNGYCREALR 120

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           L   V  G  RPN++TI ++LSAC +V HGM++HC A+KLG + DVYVAT++LTMYS C 
Sbjct: 121 LFANVGPGGFRPNSVTIASMLSACGNVEHGMEMHCLAVKLGVESDVYVATSVLTMYSNCG 180

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
            +  AAKVF++M  KN+VS+NA+ISGLL N +P  VLDIFK M  C    PNSVTL SVL
Sbjct: 181 GLFSAAKVFEEMPIKNIVSYNAFISGLLQNGVPHVVLDIFKKMRACTGEDPNSVTLLSVL 240

Query: 241 SACSNLSHLGFGRQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNL 300
           SAC++L +LGFG+Q+H L +KI+ + D ++GTALVDMYSKCG WQ A+  F E  E  NL
Sbjct: 241 SACASLLYLGFGKQVHGLMMKIEVELDTMLGTALVDMYSKCGCWQLAYGTFKELNENRNL 300

Query: 301 ITWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHR 360
            TWN++I+GMMLN Q+E A+ELFE LES   +PDS TWNSMISG ++ G+ IEAF YF R
Sbjct: 301 FTWNAMISGMMLNAQNENAVELFEQLESEGFKPDSVTWNSMISGFSQLGKAIEAFVYFRR 360

Query: 361 MQSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGH 420
           MQSAG+VPSLKS+TSLL  C++LSAL+ GKE+H  AIR+ I  D+F++TALIDMYMKCG 
Sbjct: 361 MQSAGVVPSLKSITSLLPACADLSALQCGKEVHGLAIRTSISNDLFISTALIDMYMKCGQ 420

Query: 421 LSSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLL 480
            S AR++FD F  KP D  FWNAIISGYGRNG+N+SAF IFD+MLE KVQPN ATFTSLL
Sbjct: 421 SSWARRIFDWFQIKPNDPAFWNAIISGYGRNGDNESAFGIFDQMLEAKVQPNAATFTSLL 480

Query: 481 SICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSM 540
           S+CSH G VDKGWQ FRMMN+ + L+P   H+ CMID+L R G+L +AR L++EL EPS 
Sbjct: 481 SMCSHTGLVDKGWQVFRMMNRDFGLKPNPAHFGCMIDLLGRTGRLDEARGLIQELSEPSG 540

Query: 541 SSLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMM 600
           +  ASLLGAC  H D +LG+EMA+R+SELEP+NP PFVILS IYA L RW+D E++R +M
Sbjct: 541 AVFASLLGACESHLDSQLGKEMAIRLSELEPENPTPFVILSKIYAALGRWEDAEKIRGLM 600

Query: 601 NDKGLRKPSGIS 612
           NDK LRK  G S
Sbjct: 601 NDKTLRKLPGFS 612

BLAST of Lag0040087 vs. ExPASy TrEMBL
Match: A0A2N9EBR8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10 PE=4 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 8.2e-226
Identity = 397/614 (64.66%), Postives = 481/614 (78.34%), Query Frame = 0

Query: 1   MKREIARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLI 60
           MKREIA+LV+DGF+ EA+S+YSQ+HSASL P+ F FPPL KACAKL S+ +G+MLHTHLI
Sbjct: 4   MKREIAKLVSDGFFKEAISLYSQYHSASLPPNKFTFPPLLKACAKLKSLSEGQMLHTHLI 63

Query: 61  KTGFLADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALR 120
           KTGF +DVYAAT+LTDMY+KL    DALKVFDEMP RN AS N +ISGFS NG   EA  
Sbjct: 64  KTGFSSDVYAATSLTDMYMKLHLLSDALKVFDEMPERNSASFNAVISGFSQNGYCREAFS 123

Query: 121 LAELVNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 180
           + + V  G+ RPN+ TI +LLSAC SV HGM++HC AIKLG + DVYVAT+++TMYS  E
Sbjct: 124 VFKQVGFGTFRPNSATIASLLSACESVRHGMEMHCLAIKLGVETDVYVATSIVTMYSNYE 183

Query: 181 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 240
           E+  A +VF+   NK+VVS+NA+ISGLL N + RTVLD+FK+M EC   +PNSVT   VL
Sbjct: 184 ELVSATRVFEGTPNKSVVSYNAFISGLLQNGVTRTVLDVFKDMRECSFEEPNSVTFILVL 243

Query: 241 SACSNLSHLGFGRQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNL 300
           S C++L +L FGRQ+H L +K + + D  + TALVDMYSKCG WQ A +   E     NL
Sbjct: 244 SVCASLLYLRFGRQVHGLIMKNEIEFDATLATALVDMYSKCGCWQLAFDACKEFDGIRNL 303

Query: 301 ITWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHR 360
           ITWNS+IAGMMLN QSE A+ELFE LE   L PDSATWNSMISG ++ G+ IEA KYF R
Sbjct: 304 ITWNSMIAGMMLNSQSENAIELFEQLECEGLVPDSATWNSMISGFSQLGKGIEAIKYFKR 363

Query: 361 MQSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGH 420
           M SAGIVPSLKS+TSLL  CS+LSAL+ GKEIH  AIR+ I  D FLATALIDMYMKCGH
Sbjct: 364 MVSAGIVPSLKSITSLLPACSDLSALQCGKEIHGHAIRTDISTDEFLATALIDMYMKCGH 423

Query: 421 LSSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLL 480
              A+++FDQFD KP D  FWNA+ISGYGRNGEN+SAF IFD+M EE  QPN ATF S+L
Sbjct: 424 QFLAQRVFDQFDRKPDDPAFWNAMISGYGRNGENESAFKIFDQMREENAQPNAATFISVL 483

Query: 481 SICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSM 540
           S+C H GQV+KGWQ FRMM++ Y L+P  +H  CM+D+L R+G+LG+A+EL++ELP+PS 
Sbjct: 484 SVCGHTGQVNKGWQVFRMMSRDYGLKPKPEHIGCMVDLLGRSGRLGEAKELMQELPQPSA 543

Query: 541 SSLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMM 600
           S +ASLLGAC  H D +LGEEMA+++SELEP+NP PFVILSNIYAE  RW DVER+RE+M
Sbjct: 544 SVVASLLGACKCHLDSELGEEMAMKLSELEPENPAPFVILSNIYAEFGRWGDVERIREIM 603

Query: 601 NDKGLRKPSGISSI 614
           N+KGLRK  G S I
Sbjct: 604 NNKGLRKLPGFSII 617

BLAST of Lag0040087 vs. TAIR 10
Match: AT2G02750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 1.3e-178
Identity = 301/608 (49.51%), Postives = 435/608 (71.55%), Query Frame = 0

Query: 5   IARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGF 64
           ++ LV  G  ++ +  +S        P+ F FPPL K+CAKL  V QG++LH  ++KTGF
Sbjct: 11  VSNLVTGGTSLDVILSHS--------PNKFTFPPLLKSCAKLGDVVQGRILHAQVVKTGF 70

Query: 65  LADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAEL 124
             DV+ ATAL  MY+K+    DALKV DEMP R +AS+N  +SG   NG   +A R+   
Sbjct: 71  FVDVFTATALVSMYMKVKQVTDALKVLDEMPERGIASVNAAVSGLLENGFCRDAFRMFGD 130

Query: 125 VNRGSLRPNTITIVNLLSACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGC 184
                   N++T+ ++L  C  +  GMQ+HC A+K GF+M+VYV T+L++MYS C E   
Sbjct: 131 ARVSGSGMNSVTVASVLGGCGDIEGGMQLHCLAMKSGFEMEVYVGTSLVSMYSRCGEWVL 190

Query: 185 AAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACS 244
           AA++F+ + +K+VV++NA+ISGL+ N +   V  +F  M +    +PN VT  + ++AC+
Sbjct: 191 AARMFEKVPHKSVVTYNAFISGLMENGVMNLVPSVFNLMRKFSSEEPNDVTFVNAITACA 250

Query: 245 NLSHLGFGRQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWN 304
           +L +L +GRQ+H L +K +   + +VGTAL+DMYSKC  W+ A+ +F E  +T NLI+WN
Sbjct: 251 SLLNLQYGRQLHGLVMKKEFQFETMVGTALIDMYSKCRCWKSAYIVFTELKDTRNLISWN 310

Query: 305 SLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRMQSA 364
           S+I+GMM+N Q E A+ELFE L+S  L+PDSATWNS+ISG ++ G+ IEAFK+F RM S 
Sbjct: 311 SVISGMMINGQHETAVELFEKLDSEGLKPDSATWNSLISGFSQLGKVIEAFKFFERMLSV 370

Query: 365 GIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSA 424
            +VPSLK LTSLLS CS++  L++GKEIH   I++    D+F+ T+LIDMYMKCG  S A
Sbjct: 371 VMVPSLKCLTSLLSACSDIWTLKNGKEIHGHVIKAAAERDIFVLTSLIDMYMKCGLSSWA 430

Query: 425 RKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICS 484
           R++FD+F+ KPKD +FWN +ISGYG++GE +SA +IF+ + EEKV+P++ATFT++LS CS
Sbjct: 431 RRIFDRFEPKPKDPVFWNVMISGYGKHGECESAIEIFELLREEKVEPSLATFTAVLSACS 490

Query: 485 HCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELPEPSMSSLA 544
           HCG V+KG Q FR+M ++Y  +P  +H  CMID+L R+G+L +A+E+++++ EPS S  +
Sbjct: 491 HCGNVEKGSQIFRLMQEEYGYKPSTEHIGCMIDLLGRSGRLREAKEVIDQMSEPSSSVYS 550

Query: 545 SLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKG 604
           SLLG+C  H DP LGEE A++++ELEP+NP PFVILS+IYA LERW+DVE +R++++ K 
Sbjct: 551 SLLGSCRQHLDPVLGEEAAMKLAELEPENPAPFVILSSIYAALERWEDVESIRQVIDQKQ 610

Query: 605 LRKPSGIS 612
           L K  G+S
Sbjct: 611 LVKLPGLS 610

BLAST of Lag0040087 vs. TAIR 10
Match: AT1G11290.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 3.9e-103
Identity = 212/604 (35.10%), Postives = 338/604 (55.96%), Query Frame = 0

Query: 16  EALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGFLADVYAATALT 75
           +AL  + +     + P  + F  L K C     +  GK +H  L+K+GF  D++A T L 
Sbjct: 118 KALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLE 177

Query: 76  DMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAELVNRGSLRPNTI 135
           +MY K     +A KVFD MP R+L S NT+++G+S NG    AL + + +   +L+P+ I
Sbjct: 178 NMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFI 237

Query: 136 TIVNLL---SACASVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGCAAKVFQDM 195
           TIV++L   SA   ++ G +IH +A++ GF   V ++TAL+ MY+ C  +  A ++F  M
Sbjct: 238 TIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGM 297

Query: 196 SNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACSNLSHLGFG 255
             +NVVS+N+ I   + N+ P+  + IF+ M++    KP  V++   L AC++L  L  G
Sbjct: 298 LERNVVSWNSMIDAYVQNENPKEAMLIFQKMLD-EGVKPTDVSVMGALHACADLGDLERG 357

Query: 256 RQIHALTIKIDSD-DVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWNSLIAGMML 315
           R IH L++++  D +V V  +L+ MY KC                               
Sbjct: 358 RFIHKLSVELGLDRNVSVVNSLISMYCKC------------------------------- 417

Query: 316 NDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRMQSAGIVPSLKS 375
             + + A  +F  L+S  L     +WN+MI G A++GRPI+A  YF +M+S  + P   +
Sbjct: 418 -KEVDTAASMFGKLQSRTL----VSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFT 477

Query: 376 LTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSARKLFDQFD 435
             S+++  + LS   H K IH   +RS +  +VF+ TAL+DMY KCG +  AR +FD   
Sbjct: 478 YVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMS 537

Query: 436 FKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICSHCGQVDKG 495
            +   T  WNA+I GYG +G  K+A ++F+ M +  ++PN  TF S++S CSH G V+ G
Sbjct: 538 ERHVTT--WNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAG 597

Query: 496 WQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELP-EPSMSSLASLLGACS 555
            + F MM + Y ++   DHY  M+D+L RAG+L +A + + ++P +P+++   ++LGAC 
Sbjct: 598 LKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQ 657

Query: 556 LHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKGLRKPSGI 615
           +HK+    E+ A R+ EL P +    V+L+NIY     W+ V +VR  M  +GLRK  G 
Sbjct: 658 IHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGC 682

BLAST of Lag0040087 vs. TAIR 10
Match: AT5G27110.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 6.2e-101
Identity = 210/591 (35.53%), Postives = 325/591 (54.99%), Query Frame = 0

Query: 31  PHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGFLADVYAATALTDMYVKLLHFEDALKV 90
           P +F FP + KA   L     G+M+HT ++K+G++ DV  A++L  MY K   FE++L+V
Sbjct: 105 PDSFTFPNVIKAYGALGREFLGRMIHTLVVKSGYVCDVVVASSLVGMYAKFNLFENSLQV 164

Query: 91  FDEMPHRNLASLNTMISGFSMNGSRGEALRLAELVNRGSLRPNTITIVNLLSACAS---V 150
           FDEMP R++AS NT+IS F  +G   +AL L   +      PN++++   +SAC+    +
Sbjct: 165 FDEMPERDVASWNTVISCFYQSGEAEKALELFGRMESSGFEPNSVSLTVAISACSRLLWL 224

Query: 151 AHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEEVGCAAKVFQDMSNKNVVSFNAYISGL 210
             G +IH   +K GF++D YV +AL+ MY  C+ +  A +VFQ M  K++V++N+ I G 
Sbjct: 225 ERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDCLEVAREVFQKMPRKSLVAWNSMIKGY 284

Query: 211 LLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLSACSNLSHLGFGRQIHALTIK-IDSDD 270
           +     ++ ++I  N M     +P+  TL S+L ACS   +L  G+ IH   I+ + + D
Sbjct: 285 VAKGDSKSCVEIL-NRMIIEGTRPSQTTLTSILMACSRSRNLLHGKFIHGYVIRSVVNAD 344

Query: 271 VIVGTALVDMYSKCGAWQWAHNLFNERIETNNLITWNSLIAGMMLNDQSEIAMELFESLE 330
           + V  +L+D+Y KCG    A  +F+                                   
Sbjct: 345 IYVNCSLIDLYFKCGEANLAETVFS----------------------------------- 404

Query: 331 SHKLQPDSA-TWNSMISGLARSGRPIEAFKYFHRMQSAGIVPSLKSLTSLLSICSNLSAL 390
             K Q D A +WN MIS     G   +A + + +M S G+ P + + TS+L  CS L+AL
Sbjct: 405 --KTQKDVAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAAL 464

Query: 391 RHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHLSSARKLFDQFDFKPKDTIFWNAIIS 450
             GK+IH     S +  D  L +AL+DMY KCG+   A ++F+      KD + W  +IS
Sbjct: 465 EKGKQIHLSISESRLETDELLLSALLDMYSKCGNEKEAFRIFN--SIPKKDVVSWTVMIS 524

Query: 451 GYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLSICSHCGQVDKGWQFFRMMNKKYDLQ 510
            YG +G+ + A   FD M +  ++P+  T  ++LS C H G +D+G +FF  M  KY ++
Sbjct: 525 AYGSHGQPREALYQFDEMQKFGLKPDGVTLLAVLSACGHAGLIDEGLKFFSQMRSKYGIE 584

Query: 511 PYRDHYSCMIDILSRAGQLGKARELLEELPEPSMSS--LASLLGACSLHKDPKLGEEMAL 570
           P  +HYSCMIDIL RAG+L +A E++++ PE S ++  L++L  AC LH +  LG+ +A 
Sbjct: 585 PIIEHYSCMIDILGRAGRLLEAYEIIQQTPETSDNAELLSTLFSACCLHLEHSLGDRIAR 644

Query: 571 RISELEPKNPLPFVILSNIYAELERWKDVERVREMMNDKGLRKPSGISSIE 615
            + E  P +   +++L N+YA  E W    RVR  M + GLRK  G S IE
Sbjct: 645 LLVENYPDDASTYMVLFNLYASGESWDAARRVRLKMKEMGLRKKPGCSWIE 655

BLAST of Lag0040087 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 351.7 bits (901), Expect = 1.2e-96
Identity = 204/623 (32.74%), Postives = 338/623 (54.25%), Query Frame = 0

Query: 36  FPPLFKACAKLN-SVPQGKMLHTHLIKTGFLADVYAATALTDMYVKLLHFEDALKVFDEM 95
           F  L  +C K   S    + +H  +IK+GF  +++    L D Y K    ED  +VFD+M
Sbjct: 22  FAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKM 81

Query: 96  PHRNL-------------------------------ASLNTMISGFSMNGSRGEALRLAE 155
           P RN+                                + N+M+SGF+ +    EAL    
Sbjct: 82  PQRNIYTWNSVVTGLTKLGFLDEADSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFA 141

Query: 156 LVNRGSLRPNTITIVNLLSACA---SVAHGMQIHCWAIKLGFQMDVYVATALLTMYSTCE 215
           ++++     N  +  ++LSAC+    +  G+Q+H    K  F  DVY+ +AL+ MYS C 
Sbjct: 142 MMHKEGFVLNEYSFASVLSACSGLNDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCG 201

Query: 216 EVGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVL 275
            V  A +VF +M ++NVVS+N+ I+    N      LD+F+ M+E    +P+ VTLASV+
Sbjct: 202 NVNDAQRVFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESR-VEPDEVTLASVI 261

Query: 276 SACSNLSHLGFGRQIHALTIKIDS--DDVIVGTALVDMYSKCGAWQWAHNLFNERIETNN 335
           SAC++LS +  G+++H   +K D   +D+I+  A VDMY+KC   + A  +F + +   N
Sbjct: 262 SACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIF-DSMPIRN 321

Query: 336 LITWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFH 395
           +I   S+I+G  +   ++ A  +F  +    +     +WN++I+G  ++G   EA   F 
Sbjct: 322 VIAETSMISGYAMAASTKAARLMFTKMAERNV----VSWNALIAGYTQNGENEEALSLFC 381

Query: 396 RMQSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIR------SIIHMDVFLATALID 455
            ++   + P+  S  ++L  C++L+ L  G + H   ++      S    D+F+  +LID
Sbjct: 382 LLKRESVCPTHYSFANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLID 441

Query: 456 MYMKCGHLSSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNV 515
           MY+KCG +     +F +     +D + WNA+I G+ +NG    A ++F  MLE   +P+ 
Sbjct: 442 MYVKCGCVEEGYLVFRK--MMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDH 501

Query: 516 ATFTSLLSICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLE 575
            T   +LS C H G V++G  +F  M + + + P RDHY+CM+D+L RAG L +A+ ++E
Sbjct: 502 ITMIGVLSACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIE 561

Query: 576 ELP-EPSMSSLASLLGACSLHKDPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKD 615
           E+P +P      SLL AC +H++  LG+ +A ++ E+EP N  P+V+LSN+YAEL +W+D
Sbjct: 562 EMPMQPDSVIWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWED 621

BLAST of Lag0040087 vs. TAIR 10
Match: AT3G02330.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.8e-95
Identity = 204/616 (33.12%), Postives = 330/616 (53.57%), Query Frame = 0

Query: 5   IARLVADGFYIEALSMYSQHHSASLRPHNFIFPPLFKACAKLNSVPQGKMLHTHLIKTGF 64
           IA  V +     AL  + +    +      I+  + ++CA L+ +  G  LH H +K+ F
Sbjct: 253 IAGCVQNNLLSLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDF 312

Query: 65  LADVYAATALTDMYVKLLHFEDALKVFDEMPHRNLASLNTMISGFSMNGSRGEALRLAEL 124
            AD    TA  DMY K  + +DA  +FD   + N  S N MI+G+S      +AL L   
Sbjct: 313 AADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHR 372

Query: 125 VNRGSLRPNTITIVNLLSACASV---AHGMQIHCWAIKLGFQMDVYVATALLTMYSTCEE 184
           +    L  + I++  +  ACA V   + G+QI+  AIK    +DV VA A + MY  C+ 
Sbjct: 373 LMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQA 432

Query: 185 VGCAAKVFQDMSNKNVVSFNAYISGLLLNDMPRTVLDIFKNMMECPCAKPNSVTLASVLS 244
           +  A +VF +M  ++ VS+NA I+    N      L +F +M+     +P+  T  S+L 
Sbjct: 433 LAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSR-IEPDEFTFGSILK 492

Query: 245 ACSNLSHLGFGRQIHALTIKID-SDDVIVGTALVDMYSKCGAWQWAHNLFNERIETNNLI 304
           AC+  S LG+G +IH+  +K   + +  VG +L+DMYSKCG  + A  + +   +  N  
Sbjct: 493 ACTGGS-LGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRAN-- 552

Query: 305 TWNSLIAGMMLNDQSEIAMELFESLESHKLQPDSATWNSMISGLARSGRPIEAFKYFHRM 364
                ++G          ME  E + + +LQ    +WNS+ISG     +  +A   F RM
Sbjct: 553 -----VSG---------TMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRM 612

Query: 365 QSAGIVPSLKSLTSLLSICSNLSALRHGKEIHTQAIRSIIHMDVFLATALIDMYMKCGHL 424
              GI P   +  ++L  C+NL++   GK+IH Q I+  +  DV++ + L+DMY KCG L
Sbjct: 613 MEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDL 672

Query: 425 SSARKLFDQFDFKPKDTIFWNAIISGYGRNGENKSAFDIFDRMLEEKVQPNVATFTSLLS 484
             +R +F++     +D + WNA+I GY  +G+ + A  +F+RM+ E ++PN  TF S+L 
Sbjct: 673 HDSRLMFEK--SLRRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILR 732

Query: 485 ICSHCGQVDKGWQFFRMMNKKYDLQPYRDHYSCMIDILSRAGQLGKARELLEELP-EPSM 544
            C+H G +DKG ++F MM + Y L P   HYS M+DIL ++G++ +A EL+ E+P E   
Sbjct: 733 ACAHMGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADD 792

Query: 545 SSLASLLGACSLHK-DPKLGEEMALRISELEPKNPLPFVILSNIYAELERWKDVERVREM 604
               +LLG C++H+ + ++ EE    +  L+P++   + +LSN+YA+   W+ V  +R  
Sbjct: 793 VIWRTLLGVCTIHRNNVEVAEEATAALLRLDPQDSSAYTLLSNVYADAGMWEKVSDLRRN 848

Query: 605 MNDKGLRKPSGISSIE 615
           M    L+K  G S +E
Sbjct: 853 MRGFKLKKEPGCSWVE 848

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032196.19.4e-31085.83Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_023537719.11.4e-30985.99pentatricopeptide repeat-containing protein At2g02750 [Cucurbita pepo subsp. pep... [more]
KAG6601415.17.1e-30985.83Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022998231.13.0e-30785.50pentatricopeptide repeat-containing protein At2g02750 [Cucurbita maxima][more]
XP_022956404.15.2e-30785.02pentatricopeptide repeat-containing protein At2g02750 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q1PFA61.8e-17749.51Pentatricopeptide repeat-containing protein At2g02750 OS=Arabidopsis thaliana OX... [more]
Q3E6Q15.5e-10235.10Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... [more]
O046598.8e-10035.53Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana OX... [more]
Q9SIT71.7e-9532.74Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9FWA62.5e-9433.12Pentatricopeptide repeat-containing protein At3g02330, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1K9P71.5e-30785.50pentatricopeptide repeat-containing protein At2g02750 OS=Cucurbita maxima OX=366... [more]
A0A6J1GYX92.5e-30785.02pentatricopeptide repeat-containing protein At2g02750 OS=Cucurbita moschata OX=3... [more]
A0A6J1DCC83.0e-30584.20pentatricopeptide repeat-containing protein At2g02750 OS=Momordica charantia OX=... [more]
A0A314YQJ56.3e-22664.05Pentatricopeptide repeat-containing protein OS=Prunus yedoensis var. nudiflora O... [more]
A0A2N9EBR88.2e-22664.66Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02750.11.3e-17849.51Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G11290.13.9e-10335.10Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G27110.16.2e-10135.53Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G13600.11.2e-9632.74Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G02330.11.8e-9533.12Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 408..429
e-value: 0.083
score: 13.2
coord: 72..98
e-value: 0.022
score: 15.0
coord: 510..535
e-value: 0.0025
score: 17.9
coord: 198..226
e-value: 0.053
score: 13.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 300..334
e-value: 0.0013
score: 16.7
coord: 440..472
e-value: 4.5E-7
score: 27.6
coord: 336..368
e-value: 2.0E-10
score: 38.1
coord: 474..506
e-value: 3.3E-4
score: 18.6
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 298..346
e-value: 1.0E-10
score: 41.6
coord: 435..482
e-value: 1.0E-12
score: 48.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 436..470
score: 12.112294
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 333..367
score: 13.745527
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 67..101
score: 8.61564
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 298..332
score: 9.700809
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 471..501
score: 9.393891
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..147
e-value: 5.9E-23
score: 83.7
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 483..611
e-value: 1.3E-14
score: 56.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 261..371
e-value: 2.5E-23
score: 85.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 148..250
e-value: 7.0E-17
score: 63.3
coord: 384..482
e-value: 3.3E-22
score: 80.7
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 386..574
NoneNo IPR availablePANTHERPTHR24015:SF930PPR CONTAINING PLANT-LIKE PROTEINcoord: 6..609
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 6..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040087.1Lag0040087.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009451 RNA modification
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0005515 protein binding
molecular_function GO:0003723 RNA binding