Lag0040068 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040068 vs. NCBI nr
Match: XP_038892019.1 (protein PTST homolog 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 811.2 bits (2094), Expect = 5.6e-231
Identity = 424/527 (80.46%), Postives = 463/527 (87.86%), Query Frame = 0

Query: 1   MLVFTNALPTSY-FFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGM 60
           MLVFTNALPTSY FFPT FG LSP V PSIVF+ NS+WGWNS HMAATER+C    SKGM
Sbjct: 1   MLVFTNALPTSYFFFPTDFGYLSPSVLPSIVFVPNSKWGWNSHHMAATERKCALSFSKGM 60

Query: 61  FYGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKE 120
            YG SGF+ RCK LD EGDFALEAEIL+FM SSRNPEAFPSKKDLIEAGREDLVDAIVK+
Sbjct: 61  LYGCSGFVTRCKDLDREGDFALEAEILEFMRSSRNPEAFPSKKDLIEAGREDLVDAIVKK 120

Query: 121 GGWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIAS 180
           GGWLCLGW+LDEEEDG IG F L++R SILATDW++F  SNS K DE QRFAT +SPIAS
Sbjct: 121 GGWLCLGWNLDEEEDGGIGQFCLDERESILATDWDNFGLSNSLKCDESQRFATPLSPIAS 180

Query: 181 LSTSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETV 240
            STSSSFTGRSL++VA++ESGIEGMLSRLEKERN TFGA      GKNDL PET A+ETV
Sbjct: 181 SSTSSSFTGRSLDTVAQDESGIEGMLSRLEKERNATFGA------GKNDLLPETTAKETV 240

Query: 241 ADLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQ 300
           ADLQGSTISTPLS K+ KW+DLG  L Q SSPVQ+NGLGG ++PEVQ  R+WSTFKT+LQ
Sbjct: 241 ADLQGSTISTPLSGKQDKWSDLGVCLSQGSSPVQLNGLGGSVTPEVQ--RSWSTFKTDLQ 300

Query: 301 DGDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELEL 360
           D DFEAS    S GRS EVLDV RYE+LG R D TNSN  E++M+ NQ+R +LQN+ELEL
Sbjct: 301 DKDFEASEIALSTGRSGEVLDVLRYESLGYRGDSTNSNSSEKEMNHNQLRRHLQNMELEL 360

Query: 361 SSVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKM 420
           SSV+GSLRSNA  SEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAV+EGKM
Sbjct: 361 SSVIGSLRSNAFVSEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVIEGKM 420

Query: 421 ALTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKS 480
           ALTIIDAQKVIE KQKR+NCARRALQLLR+ACVVWPNSASEVLLVGSFDGWSTQRKME+S
Sbjct: 421 ALTIIDAQKVIEGKQKRINCARRALQLLRTACVVWPNSASEVLLVGSFDGWSTQRKMERS 480

Query: 481 STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLII 523
           STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVN+SGYENNLLII
Sbjct: 481 STGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNTSGYENNLLII 519

BLAST of Lag0040068 vs. NCBI nr
Match: XP_022968253.1 (protein PTST homolog 2, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 809.3 bits (2089), Expect = 2.1e-230
Identity = 425/527 (80.65%), Postives = 462/527 (87.67%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTNALPTSYFFPT FGCLSP  FPS VF+  SR G NS HMAATERRC  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           STSSSFT RS E+VAE+ESGIEGML+RLE+ERN TFG       GKN LH ET A+ETVA
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFG------TGKNGLHSETTAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKW +LG +L +NSSPVQING GG +S EVQ  RTWSTFKT+LQD
Sbjct: 241 DLQEIPISTPLSAKEGKWTNLGSYLSKNSSPVQINGQGGSVSSEVQ--RTWSTFKTDLQD 300

Query: 301 GDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ S    S+ RS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ Y+QNL+LELS
Sbjct: 301 GDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S +GSLRSNA ASEKGQS+ SD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LR+ACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Lag0040068 vs. NCBI nr
Match: KAG6574114.1 (Protein PTST-like 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806.2 bits (2081), Expect = 1.8e-229
Identity = 422/527 (80.08%), Postives = 462/527 (87.67%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTNALPTSYFFP+ FGCLSP VFPSIVF+  SR G NS HMAATERRC  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPSPFGCLSPGVFPSIVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCK LD EG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           STSSSFT RS E+V E+ESGIEGML+RLE+ERN TFG       GKN LHPET  +ETVA
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNATFG------TGKNCLHPETTVKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKWN+LG +L +NSSPVQING GG +S EVQ   TWSTFKT+L D
Sbjct: 241 DLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQ--GTWSTFKTDLPD 300

Query: 301 GDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ S    S+GRS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ ++QNL+LELS
Sbjct: 301 GDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           SV+GSLRSN+ ASEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LR+ACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKF+VDGQWKIDPLRPIV S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFVVDGQWKIDPLRPIVRSDGYENNLLIVT 517

BLAST of Lag0040068 vs. NCBI nr
Match: XP_022151087.1 (uncharacterized protein LOC111019111 isoform X1 [Momordica charantia])

HSP 1 Score: 798.1 bits (2060), Expect = 4.9e-227
Identity = 414/527 (78.56%), Postives = 457/527 (86.72%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           MLVFTNALPT  FFP+ FGCLSP VFP++VF+SNSR GWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + DER RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASS----KGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDFEAS      GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELS
Sbjct: 301 GDFEASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S+VGSLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA
Sbjct: 361 SMVGSLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           +TIIDAQKVIEEKQKR+NCARRALQ+LR+ACVVWPNSASEV LVGSFDGW+TQRKMEKSS
Sbjct: 421 MTIIDAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of Lag0040068 vs. NCBI nr
Match: XP_022944969.1 (protein PTST homolog 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 796.2 bits (2055), Expect = 1.9e-226
Identity = 421/527 (79.89%), Postives = 459/527 (87.10%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTN LPTSYFFPT FGCLSP VFPSIVF+  SR G     MAATERRC  LSSKGMF
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFV--SRCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCK LD EG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           STSSSFT RS E+V E+ESGIEGML+RLE+ERNVTFG       GKN LHPET  +ETVA
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFG------TGKNCLHPETTVKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKWN+LG +L +NSSPVQING GG +S EVQ   TWSTFKT+L D
Sbjct: 241 DLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQ--GTWSTFKTDLPD 300

Query: 301 GDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ S    S+GRS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ ++QNL+LELS
Sbjct: 301 GDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           SV+GSLRSN+ ASEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LR+ACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of Lag0040068 vs. ExPASy Swiss-Prot
Match: Q9LFY0 (Protein PTST homolog 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST2 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 2.4e-91
Identity = 210/464 (45.26%), Postives = 284/464 (61.21%), Query Frame = 0

Query: 68  RRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEGGWLCLGW 127
           +R  G DSEG   LE EIL+FM++S  P  FPSKKDLI +GR DLV+ IV +GGWL +GW
Sbjct: 81  KRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVNQGGWLSMGW 140

Query: 128 SLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASLSTSSSFT 187
            LDE+E+       +    ++   D +   +  +  S E  +          L  SS+ +
Sbjct: 141 DLDEQEE------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNH----GDLDLSSNLS 200

Query: 188 GRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVADLQGSTI 247
             + +  + N+SGIEG+L+RLEKERN++ G         N    + +   +V     S I
Sbjct: 201 SSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGSVP--WSSRI 260

Query: 248 STPLSAKEGKWNDLGGHLGQN--SSPVQINGLGGLL-SPEVQRLRTWSTFKTELQDGDFE 307
            T    +E   +   G   Q+       ++G  GL  SP  +  RTWS  +    D DFE
Sbjct: 261 VTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAGFTDEDFE 320

Query: 308 A---SSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVGS 367
           A   SS G +    D T+ ++  S           E ++   I+  LQ L+ ELSSV+ S
Sbjct: 321 AAEISSSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQLQSELSSVLHS 380

Query: 368 LRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTI 427
           LRS  + + + K    ++  L  LSD +E++ENEI++A++KLRS RAK+AV+EGKMA+ I
Sbjct: 381 LRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVLEGKMAMAI 440

Query: 428 IDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGV 487
           IDAQ+++ EKQ+R++ A RAL+LLR+A +VWPNSASEVLL GSFDGWSTQRKM+K+  GV
Sbjct: 441 IDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQRKMKKAENGV 500

Query: 488 FSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           FSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 501 FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Lag0040068 vs. ExPASy Swiss-Prot
Match: Q10F03 (Protein FLOURY ENDOSPERM 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=FLO6 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.2e-62
Identity = 172/484 (35.54%), Postives = 262/484 (54.13%), Query Frame = 0

Query: 75  SEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEGGWLCLGWSLDEEED 134
           + G+  LE  I +FM  S  P AFP++ +L+ AGR DL  A+   GGWL LGWS    E 
Sbjct: 76  ARGEEDLEEAIYEFMRRSDKPGAFPTRAELVAAGRADLAAAVDACGGWLSLGWSSGGAEA 135

Query: 135 G----DIGLFS--LEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASLSTSSSFTG 194
           G     +G+      + G+  A    S +   +  +  R+               +S +G
Sbjct: 136 GRASSSVGVHPDYPPEAGAAAAAGGASDLAQGAVWASSRE-------------AEASPSG 195

Query: 195 RSLESVAENE-----SGIEGMLSRLEKER--------NVTFGAGFPVAVGKND---LHPE 254
           R  E+  E       +G++GML+RL++ER          + GAG     G+ D   L  +
Sbjct: 196 RQPETEEEETETKFGTGLDGMLTRLQRERERVRPPLPRSSDGAG-----GERDNVALMGQ 255

Query: 255 TAAQETVADLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWS 314
           + A    A     T   P +     ++   G L  N S   +              RTWS
Sbjct: 256 SGAPSHSATGGRYTPKVPDNGNIHSYHPQNGALEHNKSSKSLTN---------DAWRTWS 315

Query: 315 TFK---TELQDGDFEASSKGRS--EEVLDVTRYETLGSRWDPTNS------NFGEEKMDL 374
             K   ++ Q  +  +++  +S   + LD+   + +     P+N       +  +   + 
Sbjct: 316 LDKGGFSDFQAAEIHSTNSRKSFRHDGLDILAQDDVHG---PSNGVAVHDYDINDVDSER 375

Query: 375 NQIRSYLQNLELELSSVVGSLRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKD 434
           + I + LQNLEL+L++ + +LRS  + + S+  +   +     LSD +EF+E +++ A++
Sbjct: 376 DDIHARLQNLELDLTAALHTLRSRFDKVISDMSEGDGAKAPNGLSDDWEFEETKVMQAQE 435

Query: 435 KLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLL 494
           +LRSIRAKIAV+EGKMAL II+  K+IEEKQ+R++ A +AL  LR+  +VW N ASEVLL
Sbjct: 436 ELRSIRAKIAVLEGKMALEIIEKNKIIEEKQRRLDEAEKALSELRTVYIVWSNPASEVLL 495

Query: 495 VGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNL 524
            GSFDGW++QR+ME+S  G FSL L+LYPG+YEIKFIVDG W+ DPLRP+V+++G+ENNL
Sbjct: 496 TGSFDGWTSQRRMERSERGTFSLNLRLYPGRYEIKFIVDGVWRNDPLRPLVSNNGHENNL 529

BLAST of Lag0040068 vs. ExPASy Swiss-Prot
Match: F4KFB3 (Protein PTST homolog 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.7e-12
Identity = 47/153 (30.72%), Postives = 78/153 (50.98%), Query Frame = 0

Query: 379 ELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARR 438
           E+  L    + +E E+   K+++   +  ++V++ +    I  AQ +I EK+  +  A  
Sbjct: 446 EIDRLRFMLDQKELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEE 505

Query: 439 ALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYE 498
           +L  L+   + +    + V + GSF+GW  +  ME  ++        +S  L LYPG YE
Sbjct: 506 SLSGLQEVVIEYCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYE 565

Query: 499 IKFIVDGQWKIDPLRPIVNSSGYENNLLIITSK 526
           IKFIVDGQW  DP +  V      NN+L + S+
Sbjct: 566 IKFIVDGQWITDPQKDSVTRGHISNNILKVDSQ 598

BLAST of Lag0040068 vs. ExPASy Swiss-Prot
Match: Q94AX2 (Protein PTST, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTST PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 7.1e-11
Identity = 40/110 (36.36%), Postives = 63/110 (57.27%), Query Frame = 0

Query: 417 LTIIDA-QKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKS 476
           L+ +DA QK ++E+ K +  A+      +   V W   A  V ++GSFDGWS +  +   
Sbjct: 172 LSRLDAVQKKMKEQIKGVEAAQS-----KEVHVFWIGMAESVQVMGSFDGWSQREDLSPE 231

Query: 477 STGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLII 523
            + +F+ F   L L PG+YE+KF+VDG+W+I P  P       ENN+L++
Sbjct: 232 YSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVV 276

BLAST of Lag0040068 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.3e-08
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 0

Query: 430 QKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPG 489
           Q+ ++ + +  Q  R   + W     EV + GSF+ WST+  + KS    F   L L  G
Sbjct: 62  QQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHND-FVAILDLPEG 121

Query: 490 KYEIKFIVDGQWKIDPLRPIVNSS-GYENNLL 521
           +++ KF VDGQW  DP  P+V S  G  NNL+
Sbjct: 122 EHQYKFFVDGQWVHDPSEPVVTSQLGTINNLI 152

BLAST of Lag0040068 vs. ExPASy TrEMBL
Match: A0A6J1HXH5 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 1.0e-230
Identity = 425/527 (80.65%), Postives = 462/527 (87.67%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTNALPTSYFFPT FGCLSP  FPS VF+  SR G NS HMAATERRC  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           STSSSFT RS E+VAE+ESGIEGML+RLE+ERN TFG       GKN LH ET A+ETVA
Sbjct: 181 STSSSFTSRSQETVAEHESGIEGMLNRLERERNATFG------TGKNGLHSETTAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKW +LG +L +NSSPVQING GG +S EVQ  RTWSTFKT+LQD
Sbjct: 241 DLQEIPISTPLSAKEGKWTNLGSYLSKNSSPVQINGQGGSVSSEVQ--RTWSTFKTDLQD 300

Query: 301 GDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ S    S+ RS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ Y+QNL+LELS
Sbjct: 301 GDFKGSEMALSEVRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRYIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S +GSLRSNA ASEKGQS+ SD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SAIGSLRSNAFASEKGQSKPSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LR+ACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV+S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVSSDGYENNLLIVT 517

BLAST of Lag0040068 vs. ExPASy TrEMBL
Match: A0A6J1DCK2 (uncharacterized protein LOC111019111 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.4e-227
Identity = 414/527 (78.56%), Postives = 457/527 (86.72%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           MLVFTNALPT  FFP+ FGCLSP VFP++VF+SNSR GWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + DER RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASS----KGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDFEAS      GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELS
Sbjct: 301 GDFEASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           S+VGSLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA
Sbjct: 361 SMVGSLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           +TIIDAQKVIEEKQKR+NCARRALQ+LR+ACVVWPNSASEV LVGSFDGW+TQRKMEKSS
Sbjct: 421 MTIIDAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 513

BLAST of Lag0040068 vs. ExPASy TrEMBL
Match: A0A6J1FZM9 (protein PTST homolog 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449347 PE=4 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 9.0e-227
Identity = 421/527 (79.89%), Postives = 459/527 (87.10%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTN LPTSYFFPT FGCLSP VFPSIVF+  SR G     MAATERRC  LSSKGMF
Sbjct: 1   MLLFTNVLPTSYFFPTPFGCLSPGVFPSIVFV--SRCG----GMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCK LD EG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKDLDREGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           STSSSFT RS E+V E+ESGIEGML+RLE+ERNVTFG       GKN LHPET  +ETVA
Sbjct: 181 STSSSFTSRSQETVVEHESGIEGMLNRLERERNVTFG------TGKNCLHPETTVKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQ   ISTPLSAKEGKWN+LG +L +NSSPVQING GG +S EVQ   TWSTFKT+L D
Sbjct: 241 DLQEIPISTPLSAKEGKWNNLGSYLSKNSSPVQINGQGGSVSSEVQ--GTWSTFKTDLPD 300

Query: 301 GDFEAS----SKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELS 360
           GDF+ S    S+GRS EVLDV RYETLGSR DPTNSN GEE+M  NQI+ ++QNL+LELS
Sbjct: 301 GDFKGSEMALSEGRSREVLDVLRYETLGSRGDPTNSNSGEEEMSHNQIQRHIQNLQLELS 360

Query: 361 SVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420
           SV+GSLRSN+ ASEKGQS+SSD+LLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA
Sbjct: 361 SVIGSLRSNSFASEKGQSKSSDDLLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMA 420

Query: 421 LTIIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSS 480
           LTIIDAQKV+EEKQKR+NCARRALQ LR+ACVVWPNSA+EVLLVGSFDGWSTQRKME+SS
Sbjct: 421 LTIIDAQKVVEEKQKRINCARRALQFLRTACVVWPNSAAEVLLVGSFDGWSTQRKMERSS 480

Query: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIV S GYENNLLI+T
Sbjct: 481 TGVFSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVRSDGYENNLLIVT 513

BLAST of Lag0040068 vs. ExPASy TrEMBL
Match: A0A6J1HT44 (protein PTST homolog 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467545 PE=4 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 7.6e-226
Identity = 425/558 (76.16%), Postives = 462/558 (82.80%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           ML+FTNALPTSYFFPT FGCLSP  FPS VF+  SR G NS HMAATERRC  LSSKGMF
Sbjct: 1   MLLFTNALPTSYFFPTPFGCLSPCAFPSFVFV--SRCGGNSYHMAATERRCSLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SGF+RRCKGLDSEG+FALEAEIL+FMESSRNPEAFPSKKDLIEAGREDLVDAIVK+G
Sbjct: 61  YGCSGFVRRCKGLDSEGNFALEAEILEFMESSRNPEAFPSKKDLIEAGREDLVDAIVKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+L++EEDG I    LEDR SILAT W+SF + NSFKSDERQRFAT VSPIAS 
Sbjct: 121 GWLCLGWNLNDEEDGGIEQLYLEDRESILATHWDSFGEFNSFKSDERQRFATDVSPIASS 180

Query: 181 STSSSFTGRS-------------------------------LESVAENESGIEGMLSRLE 240
           STSSSFT RS                                E+VAE+ESGIEGML+RLE
Sbjct: 181 STSSSFTSRSQVFYSMPMKRNLIFYDNKMLCQDVKDTNFTNRETVAEHESGIEGMLNRLE 240

Query: 241 KERNVTFGAGFPVAVGKNDLHPETAAQETVADLQGSTISTPLSAKEGKWNDLGGHLGQNS 300
           +ERN TFG       GKN LH ET A+ETVADLQ   ISTPLSAKEGKW +LG +L +NS
Sbjct: 241 RERNATFG------TGKNGLHSETTAKETVADLQEIPISTPLSAKEGKWTNLGSYLSKNS 300

Query: 301 SPVQINGLGGLLSPEVQRLRTWSTFKTELQDGDFEAS----SKGRSEEVLDVTRYETLGS 360
           SPVQING GG +S EVQ  RTWSTFKT+LQDGDF+ S    S+ RS EVLDV RYETLGS
Sbjct: 301 SPVQINGQGGSVSSEVQ--RTWSTFKTDLQDGDFKGSEMALSEVRSREVLDVLRYETLGS 360

Query: 361 RWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVGSLRSNALASEKGQSRSSDELLELSD 420
           R DPTNSN GEE+M  NQI+ Y+QNL+LELSS +GSLRSNA ASEKGQS+ SD+LLELSD
Sbjct: 361 RGDPTNSNSGEEEMSHNQIQRYIQNLQLELSSAIGSLRSNAFASEKGQSKPSDDLLELSD 420

Query: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARRALQLLRS 480
           AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKV+EEKQKR+NCARRALQ LR+
Sbjct: 421 AFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVVEEKQKRINCARRALQFLRT 480

Query: 481 ACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 524
           ACVVWPNSA+EVLLVGSFDGWSTQRKME+SSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP
Sbjct: 481 ACVVWPNSAAEVLLVGSFDGWSTQRKMERSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDP 540

BLAST of Lag0040068 vs. ExPASy TrEMBL
Match: A0A6J1DBZ3 (uncharacterized protein LOC111019111 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019111 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.9e-225
Identity = 409/523 (78.20%), Postives = 452/523 (86.42%), Query Frame = 0

Query: 1   MLVFTNALPTSYFFPTAFGCLSPRVFPSIVFLSNSRWGWNSRHMAATERRCVFLSSKGMF 60
           MLVFTNALPT  FFP+ FGCLSP VFP++VF+SNSR GWNS H AATER CV LSSKGMF
Sbjct: 1   MLVFTNALPTFLFFPSPFGCLSPCVFPNVVFVSNSRCGWNSHHTAATERGCVLLSSKGMF 60

Query: 61  YGSSGFLRRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEG 120
           YG SG +RRCKGLDSEGDFALEA+IL+FM+SS+NPEAFPSKKDLIEAGREDLVDAI K+G
Sbjct: 61  YGCSGVVRRCKGLDSEGDFALEAKILEFMKSSKNPEAFPSKKDLIEAGREDLVDAIAKKG 120

Query: 121 GWLCLGWSLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASL 180
           GWLCLGW+LDEE D  +G +  EDR SI+ATDW+SF +SN+ + DER RFA A SP+AS 
Sbjct: 121 GWLCLGWNLDEEVDEGVGQYDFEDRYSIVATDWDSFGESNNSRRDERPRFAAAASPLASS 180

Query: 181 STSSSFTGRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVA 240
           S SSSFTGRSLE VAE ESGIEGMLSRLEKERNVTFGAGFP             A+ETVA
Sbjct: 181 SASSSFTGRSLEIVAEGESGIEGMLSRLEKERNVTFGAGFP------------GAKETVA 240

Query: 241 DLQGSTISTPLSAKEGKWNDLGGHLGQNSSPVQINGLGGLLSPEVQRLRTWSTFKTELQD 300
           DLQGS+ISTPLSAKEGKWN+LGG L  NSSPVQINGLGG +SPEVQR+  W+T+ TEL+D
Sbjct: 241 DLQGSSISTPLSAKEGKWNNLGGSLSHNSSPVQINGLGGSVSPEVQRI--WNTYNTELRD 300

Query: 301 GDFEASSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVG 360
               A   GR  EVLDV+RY TLGS  D   SN GEE+M+ +QIR +LQNLELELSS+VG
Sbjct: 301 ASENALRNGRGGEVLDVSRYGTLGSGGDLIKSNPGEEEMNYHQIRRHLQNLELELSSMVG 360

Query: 361 SLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTII 420
           SLRSN  ASEKGQSRS+D+ LELSDA+EFQENEILNAKDKLRSIRAK+AVVEGKMA+TII
Sbjct: 361 SLRSNTFASEKGQSRSADDFLELSDAYEFQENEILNAKDKLRSIRAKLAVVEGKMAMTII 420

Query: 421 DAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVF 480
           DAQKVIEEKQKR+NCARRALQ+LR+ACVVWPNSASEV LVGSFDGW+TQRKMEKSSTGVF
Sbjct: 421 DAQKVIEEKQKRINCARRALQVLRTACVVWPNSASEVFLVGSFDGWATQRKMEKSSTGVF 480

Query: 481 SLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           SLFLKLYPGKYEIKFIVDGQW+IDPLRPIVNSSGYENNLLIIT
Sbjct: 481 SLFLKLYPGKYEIKFIVDGQWRIDPLRPIVNSSGYENNLLIIT 509

BLAST of Lag0040068 vs. TAIR 10
Match: AT1G27070.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 337.8 bits (865), Expect = 1.7e-92
Identity = 210/464 (45.26%), Postives = 284/464 (61.21%), Query Frame = 0

Query: 68  RRCKGLDSEGDFALEAEILKFMESSRNPEAFPSKKDLIEAGREDLVDAIVKEGGWLCLGW 127
           +R  G DSEG   LE EIL+FM++S  P  FPSKKDLI +GR DLV+ IV +GGWL +GW
Sbjct: 81  KRNLGWDSEGSKDLETEILEFMKNSEKPGMFPSKKDLIRSGRFDLVERIVNQGGWLSMGW 140

Query: 128 SLDEEEDGDIGLFSLEDRGSILATDWNSFVKSNSFKSDERQRFATAVSPIASLSTSSSFT 187
            LDE+E+       +    ++   D +   +  +  S E  +          L  SS+ +
Sbjct: 141 DLDEQEE------KVRVNENVTPQDLHIEKQLPNCNSPEMDKTLNH----GDLDLSSNLS 200

Query: 188 GRSLESVAENESGIEGMLSRLEKERNVTFGAGFPVAVGKNDLHPETAAQETVADLQGSTI 247
             + +  + N+SGIEG+L+RLEKERN++ G         N    + +   +V     S I
Sbjct: 201 SSTEQVESRNDSGIEGILTRLEKERNLSLGISVRENGKSNGAMHDISPNGSVP--WSSRI 260

Query: 248 STPLSAKEGKWNDLGGHLGQN--SSPVQINGLGGLL-SPEVQRLRTWSTFKTELQDGDFE 307
            T    +E   +   G   Q+       ++G  GL  SP  +  RTWS  +    D DFE
Sbjct: 261 VTASEIQEVDGSRGSGEYAQSRYQGAKSVSGKPGLSDSPTSETWRTWSMRRAGFTDEDFE 320

Query: 308 A---SSKGRSEEVLDVTRYETLGSRWDPTNSNFGEEKMDLNQIRSYLQNLELELSSVVGS 367
           A   SS G +    D T+ ++  S           E ++   I+  LQ L+ ELSSV+ S
Sbjct: 321 AAEISSSGLTGVKKDDTKKDSGDSMNGKDRIASSSEDVNKTHIKHRLQQLQSELSSVLHS 380

Query: 368 LRS--NALASEKGQSRSSDELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTI 427
           LRS  + + + K    ++  L  LSD +E++ENEI++A++KLRS RAK+AV+EGKMA+ I
Sbjct: 381 LRSPPDKVVTSKDSETTAGNLENLSDDWEYKENEIIHAQNKLRSTRAKLAVLEGKMAMAI 440

Query: 428 IDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSSTGV 487
           IDAQ+++ EKQ+R++ A RAL+LLR+A +VWPNSASEVLL GSFDGWSTQRKM+K+  GV
Sbjct: 441 IDAQRIVREKQRRIDHASRALRLLRTASIVWPNSASEVLLTGSFDGWSTQRKMKKAENGV 500

Query: 488 FSLFLKLYPGKYEIKFIVDGQWKIDPLRPIVNSSGYENNLLIIT 524
           FSL LKLYPGKYEIKFIVDGQWK+DPLRPIV S GYENNLLII+
Sbjct: 501 FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSGGYENNLLIIS 532

BLAST of Lag0040068 vs. TAIR 10
Match: AT5G03420.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-13
Identity = 47/153 (30.72%), Postives = 78/153 (50.98%), Query Frame = 0

Query: 379 ELLELSDAFEFQENEILNAKDKLRSIRAKIAVVEGKMALTIIDAQKVIEEKQKRMNCARR 438
           E+  L    + +E E+   K+++   +  ++V++ +    I  AQ +I EK+  +  A  
Sbjct: 446 EIDRLRFMLDQKELELSRLKEQIEKEKLSLSVLQRQAETEIQKAQMLISEKEVELQEAEE 505

Query: 439 ALQLLRSACVVWPNSASEVLLVGSFDGWSTQRKMEKSST------GVFSLFLKLYPGKYE 498
           +L  L+   + +    + V + GSF+GW  +  ME  ++        +S  L LYPG YE
Sbjct: 506 SLSGLQEVVIEYCGDGNAVEVTGSFNGWQHRVGMELQASKSIGKQKCWSTLLWLYPGTYE 565

Query: 499 IKFIVDGQWKIDPLRPIVNSSGYENNLLIITSK 526
           IKFIVDGQW  DP +  V      NN+L + S+
Sbjct: 566 IKFIVDGQWITDPQKDSVTRGHISNNILKVDSQ 598

BLAST of Lag0040068 vs. TAIR 10
Match: AT5G39790.1 (5'-AMP-activated protein kinase-related )

HSP 1 Score: 68.6 bits (166), Expect = 1.9e-11
Identity = 53/191 (27.75%), Postives = 90/191 (47.12%), Query Frame = 0

Query: 342 NQIRSYLQNLELELSSVVGSLRSNALASEKGQSRSSDELLELSDAFEFQENEILNAKDKL 401
           ++++S L  ++ E S +V  L S A    +   R   E+  +       E E+  A  KL
Sbjct: 87  DELKSLL--IDTERSKLVKKL-SEANQQNRFLKRQEHEITNIKTELALMELEV-QALVKL 146

Query: 402 RSIRAKIAVVEGKMALT-------IIDAQKVIEEKQKRMNCARRALQLLRSACVVWPNSA 461
               A + + +G   ++       ++     +++K K       A Q  +   V W   A
Sbjct: 147 AEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQ-SKEVHVFWIGMA 206

Query: 462 SEVLLVGSFDGWSTQRKMEKSSTGVFSLF---LKLYPGKYEIKFIVDGQWKIDPLRPIVN 521
             V ++GSFDGWS +  +    + +F+ F   L L PG+YE+KF+VDG+W+I P  P   
Sbjct: 207 ESVQVMGSFDGWSQREDLSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSG 266

Query: 522 SSGYENNLLII 523
               ENN+L++
Sbjct: 267 EGLMENNVLVV 272

BLAST of Lag0040068 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-07
Identity = 36/121 (29.75%), Postives = 56/121 (46.28%), Query Frame = 0

Query: 448 VVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 507
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 76  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 135

Query: 508 PIV-NSSGYENNLLIITSKVLTVSQVPSSIIPITSANPIIDVVYFDEVLCSGRSSYSKGK 567
           P+  + +G   N+L +         VP  I  I+   P          L  G   YSK  
Sbjct: 136 PLARDDAGNTFNILDLQ------DYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEP 189

BLAST of Lag0040068 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-07
Identity = 36/121 (29.75%), Postives = 56/121 (46.28%), Query Frame = 0

Query: 448 VVWPNSASEVLLVGSFDGWSTQRKMEKSSTGVFSLFLKLYPGKYEIKFIVDGQWKIDPLR 507
           + W +   E+ + GS+D W T+ ++++S    F++   L  G YE +FIVDGQW+  P  
Sbjct: 78  ITWCHGGKEIAVEGSWDNWKTRSRLQRSGKD-FTIMKVLPSGVYEYRFIVDGQWRHAPEL 137

Query: 508 PIV-NSSGYENNLLIITSKVLTVSQVPSSIIPITSANPIIDVVYFDEVLCSGRSSYSKGK 567
           P+  + +G   N+L +         VP  I  I+   P          L  G   YSK  
Sbjct: 138 PLARDDAGNTFNILDLQ------DYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEP 191