Lag0040034 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040034
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 1
Locationchr13: 1685327 .. 1687550 (-)
RNA-Seq ExpressionLag0040034
SyntenyLag0040034
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCTGTACTTCATAATTATCACCTCCATTTTTCTGCCTTTCAGCTTCTCTCTGAAGCTCGAAGCTACCATCCATGGAACAAGCCCGAGTTCCATTCAGACCAATCAAAATTCTTCACTCAGACACCAAATTCTGGAGCTCGCCAATGATCCCACTACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCATATGAAGAATTTCAAACCAGTAGGCTCATTAGAGAGGAGCTTGACAATCTCGGCGTTTCTTATCGGTGGCCAGTCGCCGGTACCGGCGTAGTGGCTACGGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTGCCTATTCAGGTCAGATTTCGTTCATTTCATTTTTCTCCTTCCTCCGCCATTAAATCCTATCTTCTGCTCGACGAAATGACCCAACGAACTTGATTGGTTCTTCGTCATCTCCATTTTTAGATCCGGCTTAATGAGAATCTGCAATCTGTTCCTCATCAGTTTAATCGTAATTTTTTTACATGACCAACATAATTGGATTATGAATGGTCCGCGGAAGACTATTTCGTTGATGATAATGCAGAACTAAATAGGTCTAAAACATGACAGAGTTATTAATTCAACAAACAATTTATAAATAATTTTGAAGTGCAAGAGGACAAATAACGATTCTGTTCGATTAGGCAAAGATGGTTAATGACCAGCCATTGCAATTATAGAATTCTTTTTATTAACTTTCTTTTTGTACCGTGGAACTGGACCATATAGTGATGAACAATGCAATGCAGCAAGATAAAAAAGTGAAGTTTTCATAAGATGGGTGTAAACTAAAATGAAAGAAGTTTTGTTTTTCTTCATTGATTTTTGATATAGGAATTGGTAGATTGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGCGCTACTAAAATTCTGCATCAACTGCGACAGAAATTACAGGTTCTCAAAGAGCATTACAAGTACTCCCCAGTGTCAACTCCAATAACCTTTTGATTAATCTGTTTAGAAAGATGGTTATGACTCAAACTTGCATCCTTTTTTGTTAATATGAACATGCCCCTTCAATGCAGGGCACTGTTGTTCTTATTTTTCAGCCGGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAAAGAGGGCGTCCTTGATGGTGTAGGAGCTATATTTGGGCTGCACATTATGCATAAATATCCGGTCGGCGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGCTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTTTGCAAAACATAGTCTCAAGAGAGATAGACCCTCTAGATTCCCAGGTACTTTCTTTCAGATGAACATTAATGATAAAAGTCTCAAATGGATTCAATTCAGCCTTGTGTAACTCTCTGGGATGATCTGATCATACAAACAAGACCGTCTATTTATTTTCATCTTAAAGGAGAGTTGAAAGTTGCAGGTGGTTTCGGTAGCCATGGTTCAAGCAGGATCTGCACTCAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGACAGGATAAAAGAGGTAACCGCAAGTACATGTTCTTCTACAGAACAAACGAATTGTCACTGCACTTTCTGACTGAACTTTTATGTTCATTTCTTATGGCAGGTCATAAATGGGCAGGCAGCGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACAATTCCTCCGACTGTAAATGACAAGAAAATTTATGAACATGCTCGACGTGTTTCTATCGAAATTGTAGGAAAGGAAAACACAAAAGTATCTCCAAACTTCATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAGAGTGCCAGGATCTTTCTTTTTTCTAGGCACATATAATGAGAGGATCGGAGCTATATATCCTCCACACAGCCCTCACTATAAAATTGATGAAAATGTGCTTCCTATCGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAGCATCCCATTCTTGCTGTGACCATTGA

mRNA sequence

ATGGAGTTTCTGTACTTCATAATTATCACCTCCATTTTTCTGCCTTTCAGCTTCTCTCTGAAGCTCGAAGCTACCATCCATGGAACAAGCCCGAGTTCCATTCAGACCAATCAAAATTCTTCACTCAGACACCAAATTCTGGAGCTCGCCAATGATCCCACTACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCATATGAAGAATTTCAAACCAGTAGGCTCATTAGAGAGGAGCTTGACAATCTCGGCGTTTCTTATCGGTGGCCAGTCGCCGGTACCGGCGTAGTGGCTACGGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTGCCTATTCAGGAATTGGTAGATTGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGCGCTACTAAAATTCTGCATCAACTGCGACAGAAATTACAGGGCACTGTTGTTCTTATTTTTCAGCCGGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAAAGAGGGCGTCCTTGATGGTGTAGGAGCTATATTTGGGCTGCACATTATGCATAAATATCCGGTCGGCGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGCTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTTTGCAAAACATAGTCTCAAGAGAGATAGACCCTCTAGATTCCCAGGTGGTTTCGGTAGCCATGGTTCAAGCAGGATCTGCACTCAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGACAGGATAAAAGAGGTCATAAATGGGCAGGCAGCGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACAATTCCTCCGACTGTAAATGACAAGAAAATTTATGAACATGCTCGACGTGTTTCTATCGAAATTGTAGGAAAGGAAAACACAAAAGTATCTCCAAACTTCATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAGAGTGCCAGGATCTTTCTTTTTTCTAGGCACATATAATGAGAGGATCGGAGCTATATATCCTCCACACAGCCCTCACTATAAAATTGATGAAAATGTGCTTCCTATCGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAGCATCCCATTCTTGCTGTGACCATTGA

Coding sequence (CDS)

ATGGAGTTTCTGTACTTCATAATTATCACCTCCATTTTTCTGCCTTTCAGCTTCTCTCTGAAGCTCGAAGCTACCATCCATGGAACAAGCCCGAGTTCCATTCAGACCAATCAAAATTCTTCACTCAGACACCAAATTCTGGAGCTCGCCAATGATCCCACTACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCATATGAAGAATTTCAAACCAGTAGGCTCATTAGAGAGGAGCTTGACAATCTCGGCGTTTCTTATCGGTGGCCAGTCGCCGGTACCGGCGTAGTGGCTACGGTCGGCTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTGCCTATTCAGGAATTGGTAGATTGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTTGCTATGCTCCTTGGCGCTACTAAAATTCTGCATCAACTGCGACAGAAATTACAGGGCACTGTTGTTCTTATTTTTCAGCCGGCGGAGGAGAGAGGAGGAGGAGCAAAAGACATGATAAAAGAGGGCGTCCTTGATGGTGTAGGAGCTATATTTGGGCTGCACATTATGCATAAATATCCGGTCGGCGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGCTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGTCATGCTGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTTTGCAAAACATAGTCTCAAGAGAGATAGACCCTCTAGATTCCCAGGTGGTTTCGGTAGCCATGGTTCAAGCAGGATCTGCACTCAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGACAGGATAAAAGAGGTCATAAATGGGCAGGCAGCGGTGCATCGGTGCACGGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACAATTCCTCCGACTGTAAATGACAAGAAAATTTATGAACATGCTCGACGTGTTTCTATCGAAATTGTAGGAAAGGAAAACACAAAAGTATCTCCAAACTTCATGGGAAGTGAAGATTTTGCTTTCTTTTCAGACAGAGTGCCAGGATCTTTCTTTTTTCTAGGCACATATAATGAGAGGATCGGAGCTATATATCCTCCACACAGCCCTCACTATAAAATTGATGAAAATGTGCTTCCTATCGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAGCATCCCATTCTTGCTGTGACCATTGA

Protein sequence

MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSSASHSCCDH
Homology
BLAST of Lag0040034 vs. NCBI nr
Match: XP_022941713.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita moschata] >KAG6600138.1 IAA-amino acid hydrolase ILR1-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030803.1 IAA-amino acid hydrolase ILR1-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.9 bits (2049), Expect = 7.2e-226
Identity = 396/441 (89.80%), Postives = 420/441 (95.24%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           ME LYF+II SIF       KLEA +HGT+ SS+QT+QNSSLRHQILELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEF+TSRLIR+ELDNLGVSYRWPVAGTG+VA+VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGV  I GLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAG+ALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAF KKSFNALRDRI+E+INGQAAVHRCT EIDFLGKEHPTIPPT+N++K+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKENTKVSPNFMGSEDFAFFSDRVPGSF FLGTYNERIGAIYPPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSAS 442
           IGAAIHAAFAYSYLLNSSS S
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lag0040034 vs. NCBI nr
Match: XP_022985573.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita maxima])

HSP 1 Score: 789.6 bits (2038), Expect = 1.4e-224
Identity = 395/441 (89.57%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           ME LYF+II SIF       KLEA +HGT+ SS+QT+QNSSLRHQILELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEF+TSRLIR ELDNLGVSYRWPVAGTG+VA+VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRLELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGV  I GLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAG+ALNVIPD  TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDLVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAFSKKSFNALRDRI+E+I+GQAAVHRCT EIDFLGKEHPTIPPT+N++K+Y+HARRVSI
Sbjct: 301 RAFSKKSFNALRDRIEEIISGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKENTKVSPNFMGSEDFAFFSDRVPGSF FLGTYNERIGAIYPPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFIFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSAS 442
           IGAAIHAAFAYSYLLNSSS S
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lag0040034 vs. NCBI nr
Match: XP_023527394.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.5 bits (2035), Expect = 3.0e-224
Identity = 394/441 (89.34%), Postives = 417/441 (94.56%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           ME LYF+II SIF       KLEA +HGT  SS+QT+QNSSLRHQILELANDP TSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTISSSVQTSQNSSLRHQILELANDPITSNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEF+TSRLIR+ELDNLGVSYRWPVAGTG+VA+VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGV  I GLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLD QVVSVAMVQAG+ALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDPQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAF KKSFNALRDRI+E+INGQAAVHRCT EIDFLGKEHPTIPPT+N++K+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKENTKVSPNFMGSEDFAFFSDRVPGSF FLGTYNERIGAIYPPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSAS 442
           IGAAIHAAFAYSYLLNSSS S
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lag0040034 vs. NCBI nr
Match: XP_038893400.1 (IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida])

HSP 1 Score: 776.5 bits (2004), Expect = 1.2e-220
Identity = 393/447 (87.92%), Postives = 418/447 (93.51%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           MEFLY IIITSIFLP +FSL  EAT+H T+P S+QTN +SSLRHQIL+ AN PTT NWMK
Sbjct: 1   MEFLY-IIITSIFLPLTFSLNFEATLHVTTPISLQTNHDSSLRHQILDFANHPTTVNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELA+EEF+TSRLIR ELDNL VSYRWPVAGTGVVA VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAFEEFETSRLIRHELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGA KIL+QLRQKLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGAAKILNQLRQKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERG GAKDMIKEGVLDGV AIF LH++HKYPVGVVASR GEFLAGCGSFK KIKGKGGHA
Sbjct: 181 ERGRGAKDMIKEGVLDGVEAIFALHLVHKYPVGVVASRAGEFLAGCGSFKVKIKGKGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAG+ALNVIPDSATIAGTF
Sbjct: 241 AIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAFSKKSFNALRDRI+EVINGQAAVHRCTAEIDFLGKEHPTIPPT+ND+KIYEH RRVS+
Sbjct: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRRVSM 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKE  + SP+ MGSEDFAFFSD+VPGSF FLGTYNERIGAI+PPHSPHYKIDENVLP
Sbjct: 361 EIVGKEKIEESPSLMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSASHSCCDH 448
           IGAAIHAAFAYSYLLNSSS SHS  DH
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTSHSFFDH 446

BLAST of Lag0040034 vs. NCBI nr
Match: KAA0034344.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa])

HSP 1 Score: 761.9 bits (1966), Expect = 3.0e-216
Identity = 383/448 (85.49%), Postives = 415/448 (92.63%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEA-TIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWM 60
           MEFLY +   SIFLP SFSL LE+  ++ T+  S+ TN  SSLRHQIL+LAN PTT NWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEF+TSRLIR+ELDNL VSYRWPVAGTGVVA VGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPI+ELV+WEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGV AIFGLH++HKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAG+ALNVIP+SATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVS 360
           FRAFSKKSFNALRDRI+EVINGQA VHRCTAEIDFLGKE+PTIPP +ND+KIYEH RRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 IEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVL 420
           +EIVGKE TKVSP+ MGSEDFAFF+D+VPGSF FLGTYNERIGAI+PPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAFAYSYLLNSSSASHSCCDH 448
           PIGAAIHAA AYSYLLNSSS SHS  DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lag0040034 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.3e-132
Identity = 234/391 (59.85%), Postives = 292/391 (74.68%), Query Frame = 0

Query: 46  ILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVA 105
           +L  A +   + WM  +RR+IHENPEL YEEF TS L+R ELD LG+ YR P A TGVVA
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 106 TVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR 165
           TVG+G PPFVALRADMDALP+QE V+WEHKSKV GKMH C HDAHVAMLLG+ +IL + R
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 166 QKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAG 225
            +L+GTVVL+FQPAEE GGGAK MI +G ++ + AIFG+H+    P+GVVASRPG  +AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 226 CGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGS 285
            G F+A I GKGGHAA+P H+IDP+LAAS  IVSLQ +VSRE DPLDSQVV+V   Q G 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 286 ALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPT 345
           A NVIPDS TI GTFRAF K+SFN L+ RI+EVI  QA+V RC A +DFL K+ P  PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 346 VNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGS-FFFLGTYNERIGAI 405
           +N   +++   +V+ E+VG +N +     MG+EDFAF++D +P + ++FLG YNE  G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 406 YPPHSPHYKIDENVLPIGAAIHAAFAYSYLL 436
            P HSP++ I+E+ LP GAA+ A+ A  YLL
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLATRYLL 426

BLAST of Lag0040034 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.3e-132
Identity = 234/391 (59.85%), Postives = 292/391 (74.68%), Query Frame = 0

Query: 46  ILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVA 105
           +L  A +   + WM  +RR+IHENPEL YEEF TS L+R ELD LG+ YR P A TGVVA
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 106 TVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR 165
           TVG+G PPFVALRADMDALP+QE V+WEHKSKV GKMH C HDAHVAMLLG+ +IL + R
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 166 QKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAG 225
            +L+GTVVL+FQPAEE GGGAK MI +G ++ + AIFG+H+    P+GVVASRPG  +AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 226 CGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGS 285
            G F+A I GKGGHAA+P H+IDP+LAAS  IVSLQ +VSRE DPLDSQVV+V   Q G 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 286 ALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPT 345
           A NVIPDS TI GTFRAF K+SFN L+ RI+EVI  QA+V RC A +DFL K+ P  PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 346 VNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGS-FFFLGTYNERIGAI 405
           +N   +++   +V+ E+VG +N +     MG+EDFAF++D +P + ++FLG YNE  G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 406 YPPHSPHYKIDENVLPIGAAIHAAFAYSYLL 436
            P HSP++ I+E+ LP GAA+ A+ A  YLL
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLAARYLL 426

BLAST of Lag0040034 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-130
Identity = 234/418 (55.98%), Postives = 307/418 (73.44%), Query Frame = 0

Query: 26  IHGTSPSSIQTNQN--SSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLI 85
           +H  +P+ I  + N  S +  + L LA      +WM  IRR+IHENPEL YEE +TS+L+
Sbjct: 13  LHLLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLV 72

Query: 86  REELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMH 145
           R EL+ +GVSY++PVA TGVV  VG+G  PFVALRADMDAL +QE+V+WEHKSKV GKMH
Sbjct: 73  RAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMH 132

Query: 146 ACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFG 205
           AC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK +++ GVL+ V AIFG
Sbjct: 133 ACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFG 192

Query: 206 LHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNI 265
           LH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDP+LAAS  IVSLQ++
Sbjct: 193 LHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHL 252

Query: 266 VSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQA 325
           VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  L+ RI++VI  QA
Sbjct: 253 VSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQA 312

Query: 326 AVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFF 385
           +V+ C A +DF+ +E P  PPTVNDK +++  + VS +++G EN       MGSEDF+F+
Sbjct: 313 SVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFY 372

Query: 386 SDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSSAS 442
              +PG F F+G  N+    +  PHSP+++++E +LP GA++HA+ A  YLL   +++
Sbjct: 373 QQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYLLELKAST 430

BLAST of Lag0040034 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 452.6 bits (1163), Expect = 5.2e-126
Identity = 233/411 (56.69%), Postives = 293/411 (71.29%), Query Frame = 0

Query: 29  TSPSSIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELD 88
           +S S       S ++ ++LE A  P   +WM  IRRKIHENPEL YEE +TS+LIR EL+
Sbjct: 21  SSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELE 80

Query: 89  NLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHD 148
            +G+ YR+PVA TGV+  +G+G PPFVALRADMDALPIQE V+WEHKSK+ GKMHAC HD
Sbjct: 81  LIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHD 140

Query: 149 AHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMH 208
            HV MLLGA KILH+ R  LQGTVVLIFQPAEE   GAK M +EG L  V AIFG+H+  
Sbjct: 141 GHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSA 200

Query: 209 KYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREI 268
           + P G  ASR G FLAG G F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ +VSRE 
Sbjct: 201 RIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRET 260

Query: 269 DPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRC 328
           DPLDS+VV+V+ V  G+A NVIPDS TI GT RAF+   F  L+ R+KEVI  QAAVHRC
Sbjct: 261 DPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQRVKEVITKQAAVHRC 320

Query: 329 TAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVP 388
            A ++        +PPTVN+K +Y+  ++V  +++G+E    +   MGSEDF++F++ +P
Sbjct: 321 NASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIP 380

Query: 389 GSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
           G F  LG  +E  G     HSP Y+I+E+VLP GAAIHA+ A  YL   +S
Sbjct: 381 GHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKAS 428

BLAST of Lag0040034 vs. ExPASy Swiss-Prot
Match: Q9SWX9 (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.2e-124
Identity = 226/408 (55.39%), Postives = 294/408 (72.06%), Query Frame = 0

Query: 33  SIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGV 92
           S  +N  S +    L LA      +WM  IRR+IHENPEL YEE +TS+L++ ELD +GV
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 93  SYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVA 152
           SY+ PVA TGV+  VG+G  PFVALRADMDALPIQE+V+WEHKSK+ GKMHAC HDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 153 MLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPV 212
           MLLGA K+L + +++LQGTV+L+FQPAEE G GAK +++ GVL+ VGAIFGLH+ +   +
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 213 GVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLD 272
           G ++SR G  +AG G FKA I GKGGHAA+PQ +IDPVLAAS  I+SLQ++VSRE DPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 273 SQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEI 332
           SQVV+VA  +   A NVIPDS TI GTFRA   KSF  L+ RI +VI  QA+V+ C A +
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 333 DFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFF 392
           DFL  E P  PPTVN+K ++   + VS++++G EN   +   M SEDFAF+   +PG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 393 FLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSSA 441
           F+G  N+    +  PHSP ++++E +LP GA++ A+ A  YLL+SSS+
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSSS 429

BLAST of Lag0040034 vs. ExPASy TrEMBL
Match: A0A6J1FSW3 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111446993 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 396/441 (89.80%), Postives = 420/441 (95.24%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           ME LYF+II SIF       KLEA +HGT+ SS+QT+QNSSLRHQILELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEF+TSRLIR+ELDNLGVSYRWPVAGTG+VA+VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGV  I GLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAG+ALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAF KKSFNALRDRI+E+INGQAAVHRCT EIDFLGKEHPTIPPT+N++K+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKENTKVSPNFMGSEDFAFFSDRVPGSF FLGTYNERIGAIYPPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSAS 442
           IGAAIHAAFAYSYLLNSSS S
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lag0040034 vs. ExPASy TrEMBL
Match: A0A6J1JE04 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111483601 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.6e-225
Identity = 395/441 (89.57%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMK 60
           ME LYF+II SIF       KLEA +HGT+ SS+QT+QNSSLRHQILELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEF+TSRLIR ELDNLGVSYRWPVAGTG+VA+VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRLELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPI+ELV+WEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGV  I GLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAG+ALNVIPD  TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDLVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSI 360
           RAFSKKSFNALRDRI+E+I+GQAAVHRCT EIDFLGKEHPTIPPT+N++K+Y+HARRVSI
Sbjct: 301 RAFSKKSFNALRDRIEEIISGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLP 420
           EIVGKENTKVSPNFMGSEDFAFFSDRVPGSF FLGTYNERIGAIYPPHSPH+KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFIFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSAS 442
           IGAAIHAAFAYSYLLNSSS S
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Lag0040034 vs. ExPASy TrEMBL
Match: A0A5A7ST02 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003470 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.5e-216
Identity = 383/448 (85.49%), Postives = 415/448 (92.63%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEA-TIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWM 60
           MEFLY +   SIFLP SFSL LE+  ++ T+  S+ TN  SSLRHQIL+LAN PTT NWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEF+TSRLIR+ELDNL VSYRWPVAGTGVVA VGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPI+ELV+WEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGV AIFGLH++HKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAG+ALNVIP+SATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVS 360
           FRAFSKKSFNALRDRI+EVINGQA VHRCTAEIDFLGKE+PTIPP +ND+KIYEH RRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 IEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVL 420
           +EIVGKE TKVSP+ MGSEDFAFF+D+VPGSF FLGTYNERIGAI+PPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAFAYSYLLNSSSASHSCCDH 448
           PIGAAIHAA AYSYLLNSSS SHS  DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lag0040034 vs. ExPASy TrEMBL
Match: A0A5D3CVM4 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00690 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 382/448 (85.27%), Postives = 414/448 (92.41%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEA-TIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWM 60
           MEFLY +   SIFLP SFSL LE+  ++ T+  S+ TN  SSLRHQIL+LAN PTT NWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEF+TSRLIR+ELDNL VSYRWPVAGTGVVA VGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPI+ELV+WEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGV AIFGLH++HKYPVGVVASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAG+ALNVIP+SATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVS 360
           FRAFSKKSFNALRDRI+EVINGQA VHRCTAEIDFLGKE+PTIPP +ND+KIYEH RRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 IEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVL 420
           +EIVGKE TKVSP+ MGSEDFAFF+D+VPGSF FLGTYNERIGAI+PPHSPHYKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAFAYSYLLNSSSASHSCCDH 448
           PIGAAIHAA AYSYLLNSSS SHS  DH
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTSHSFFDH 478

BLAST of Lag0040034 vs. ExPASy TrEMBL
Match: A0A0A0KQC3 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.1e-215
Identity = 380/448 (84.82%), Postives = 412/448 (91.96%), Query Frame = 0

Query: 1   MEFLYFIIITSIFLPFSFSLKLEA-TIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWM 60
           MEFLY +   SIFLP +FSL LE+  +HGT+  S+ TN  SSL HQI++LAN PT  NWM
Sbjct: 35  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 94

Query: 61  KTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEF+TSRLIR+ELDNL VSYRWPVAGTGVVA VGSGSPPFVALRA
Sbjct: 95  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 154

Query: 121 DMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPI+ELV+WEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 155 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 214

Query: 181 EERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGV AIFGLH++H+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 215 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 274

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAG+ALNVIP+SATIAGT
Sbjct: 275 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 334

Query: 301 FRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVS 360
           FRAFSKKSFNALRDRI+EVINGQA VHRCTAEIDFLGKEHPTIPP VND+KIYEH RRVS
Sbjct: 335 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 394

Query: 361 IEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVL 420
           +EIVGKE TKVSP  MGSEDFAFF+D+VPGSF FLGTYNERIGAI+PPHSP YKIDENVL
Sbjct: 395 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 454

Query: 421 PIGAAIHAAFAYSYLLNSSSASHSCCDH 448
           P+GAAIHAA AYSYLLNSSS SHS  DH
Sbjct: 455 PLGAAIHAAVAYSYLLNSSSTSHSFFDH 482

BLAST of Lag0040034 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-131
Identity = 234/418 (55.98%), Postives = 307/418 (73.44%), Query Frame = 0

Query: 26  IHGTSPSSIQTNQN--SSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLI 85
           +H  +P+ I  + N  S +  + L LA      +WM  IRR+IHENPEL YEE +TS+L+
Sbjct: 13  LHLLNPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLV 72

Query: 86  REELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMH 145
           R EL+ +GVSY++PVA TGVV  VG+G  PFVALRADMDAL +QE+V+WEHKSKV GKMH
Sbjct: 73  RAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMH 132

Query: 146 ACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFG 205
           AC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK +++ GVL+ V AIFG
Sbjct: 133 ACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFG 192

Query: 206 LHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNI 265
           LH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDP+LAAS  IVSLQ++
Sbjct: 193 LHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHL 252

Query: 266 VSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQA 325
           VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  L+ RI++VI  QA
Sbjct: 253 VSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQA 312

Query: 326 AVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFF 385
           +V+ C A +DF+ +E P  PPTVNDK +++  + VS +++G EN       MGSEDF+F+
Sbjct: 313 SVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFY 372

Query: 386 SDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSSAS 442
              +PG F F+G  N+    +  PHSP+++++E +LP GA++HA+ A  YLL   +++
Sbjct: 373 QQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYLLELKAST 430

BLAST of Lag0040034 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 452.6 bits (1163), Expect = 3.7e-127
Identity = 233/411 (56.69%), Postives = 293/411 (71.29%), Query Frame = 0

Query: 29  TSPSSIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELD 88
           +S S       S ++ ++LE A  P   +WM  IRRKIHENPEL YEE +TS+LIR EL+
Sbjct: 21  SSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELE 80

Query: 89  NLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHD 148
            +G+ YR+PVA TGV+  +G+G PPFVALRADMDALPIQE V+WEHKSK+ GKMHAC HD
Sbjct: 81  LIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHD 140

Query: 149 AHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMH 208
            HV MLLGA KILH+ R  LQGTVVLIFQPAEE   GAK M +EG L  V AIFG+H+  
Sbjct: 141 GHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSA 200

Query: 209 KYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREI 268
           + P G  ASR G FLAG G F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ +VSRE 
Sbjct: 201 RIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRET 260

Query: 269 DPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRC 328
           DPLDS+VV+V+ V  G+A NVIPDS TI GT RAF+   F  L+ R+KEVI  QAAVHRC
Sbjct: 261 DPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQRVKEVITKQAAVHRC 320

Query: 329 TAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVP 388
            A ++        +PPTVN+K +Y+  ++V  +++G+E    +   MGSEDF++F++ +P
Sbjct: 321 NASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIP 380

Query: 389 GSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
           G F  LG  +E  G     HSP Y+I+E+VLP GAAIHA+ A  YL   +S
Sbjct: 381 GHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKAS 428

BLAST of Lag0040034 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 447.2 bits (1149), Expect = 1.5e-125
Identity = 226/408 (55.39%), Postives = 294/408 (72.06%), Query Frame = 0

Query: 33  SIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGV 92
           S  +N  S +    L LA      +WM  IRR+IHENPEL YEE +TS+L++ ELD +GV
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 93  SYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVA 152
           SY+ PVA TGV+  VG+G  PFVALRADMDALPIQE+V+WEHKSK+ GKMHAC HDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 153 MLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPV 212
           MLLGA K+L + +++LQGTV+L+FQPAEE G GAK +++ GVL+ VGAIFGLH+ +   +
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 213 GVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLD 272
           G ++SR G  +AG G FKA I GKGGHAA+PQ +IDPVLAAS  I+SLQ++VSRE DPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 273 SQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEI 332
           SQVV+VA  +   A NVIPDS TI GTFRA   KSF  L+ RI +VI  QA+V+ C A +
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 333 DFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFF 392
           DFL  E P  PPTVN+K ++   + VS++++G EN   +   M SEDFAF+   +PG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 393 FLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSSA 441
           F+G  N+    +  PHSP ++++E +LP GA++ A+ A  YLL+SSS+
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSSS 429

BLAST of Lag0040034 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 442.6 bits (1137), Expect = 3.8e-124
Identity = 239/427 (55.97%), Postives = 297/427 (69.56%), Query Frame = 0

Query: 13  FLPFSFSLKLEATIHGTSPSSIQTNQNSSLRHQILELANDPTTSNWMKTIRRKIHENPEL 72
           FL F   L L   +   SP  I     S +    LELA  P   + M  IRRKIHENPEL
Sbjct: 6   FLTFQLLLLL-LRVSSESP-WIVAGDVSRIPINFLELAKSPEVFDSMVRIRRKIHENPEL 65

Query: 73  AYEEFQTSRLIREELDNLGVSYRWPVAGTGVVATVGSGSPPFVALRADMDALPIQELVDW 132
            YEEF+TS+ IR ELD +GV YR+PVA TG++  +G+G PPFVALRADMDALPIQE V+W
Sbjct: 66  GYEEFETSKFIRSELDLIGVKYRFPVAITGIIGYIGTGEPPFVALRADMDALPIQEAVEW 125

Query: 133 EHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKE 192
           EHKSK  GKMHAC HD HVAMLLGA KIL Q RQ LQGTVVLIFQPAEE   GAK M +E
Sbjct: 126 EHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREE 185

Query: 193 GVLDGVGAIFGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLA 252
           G L  V AIFG+H+  + P G  AS  G F+AG G+F+A I GKGGHAAIPQH+IDPV+A
Sbjct: 186 GALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVA 245

Query: 253 ASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGSALNVIPDSATIAGTFRAFSKKSFNALR 312
           AS+ ++SLQ++VSRE DP DS+VV+V  V  G+A NVIPDS TI GT RAF+   F  L+
Sbjct: 246 ASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQ 305

Query: 313 DRIKEVINGQAAVHRCTAEIDFLGKEHPTIPPTVNDKKIYEHARRVSIEIVGKENTKVSP 372
           +RIKE+I  QAAVHRC A ++     +  +PPTVN+  +Y+  ++V  +++G+E    + 
Sbjct: 306 ERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAV 365

Query: 373 NFMGSEDFAFFSDRVPGSFFFLGTYNERIGAIYPPHSPHYKIDENVLPIGAAIHAAFAYS 432
             MGSEDF++F++ +PG F  LG  +E  G     HSPHY+I+E+VLP GAAIHA  A  
Sbjct: 366 PEMGSEDFSYFAETIPGHFSLLGMQDETQG-YASSHSPHYRINEDVLPYGAAIHATMAVQ 425

Query: 433 YLLNSSS 440
           YL + +S
Sbjct: 426 YLKDKAS 427

BLAST of Lag0040034 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 433.3 bits (1113), Expect = 2.3e-121
Identity = 212/395 (53.67%), Postives = 279/395 (70.63%), Query Frame = 0

Query: 45  QILELANDPTTSNWMKTIRRKIHENPELAYEEFQTSRLIREELDNLGVSYRWPVAGTGVV 104
           +IL L   P    W+K +RR IHENPELA+EE++TSRLIR ELD +G+ YR+P+A TG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 105 ATVGSGSPPFVALRADMDALPIQELVDWEHKSKVKGKMHACSHDAHVAMLLGATKILHQL 164
           A +GSG PPFVA+RADMDALPIQE V+WEH SKV GKMHAC HDAHV MLLGA  IL   
Sbjct: 135 AWIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAR 194

Query: 165 RQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVGAIFGLHIMHKYPVGVVASRPGEFLA 224
              L+GTVVL+FQPAEE G GAK+MI++G LD V AIF +H+ H +P GV+ SR G  LA
Sbjct: 195 EHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLA 254

Query: 225 GCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAG 284
           GCG F+A I  +    A      + +LAAS+A++SLQ IVSRE  PLDSQVVSV     G
Sbjct: 255 GCGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGG 314

Query: 285 SALNVIPDSATIAGTFRAFSKKSFNALRDRIKEVINGQAAVHRCTAEIDFLGKEHPTIPP 344
            +L+V PD+  + GTFRAFS  SF  L+ RI+EV+  Q  V  C A ++F  K++   PP
Sbjct: 315 HSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPP 374

Query: 345 TVNDKKIYEHARRVSIEIVGKENTKVSPNFMGSEDFAFFSDRVPGSFFFLGTYNERIGAI 404
           T N+   Y H ++V+I+++G  +  ++P  MG+EDFAF+S+ +P +F+F+G  NE +G++
Sbjct: 375 TTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSV 434

Query: 405 YPPHSPHYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
           +  HSPH+ IDE+ LP+GAA+HAA A  YL +  S
Sbjct: 435 HIAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKHS 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022941713.17.2e-22689.80IAA-amino acid hydrolase ILR1-like 1 [Cucurbita moschata] >KAG6600138.1 IAA-amin... [more]
XP_022985573.11.4e-22489.57IAA-amino acid hydrolase ILR1-like 1 [Cucurbita maxima][more]
XP_023527394.13.0e-22489.34IAA-amino acid hydrolase ILR1-like 1 [Cucurbita pepo subsp. pepo][more]
XP_038893400.11.2e-22087.92IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida][more]
KAA0034344.13.0e-21685.49IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q84XG91.3e-13259.85IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S41.3e-13259.85IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
O043731.6e-13055.98IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
P549705.2e-12656.69IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Q9SWX92.2e-12455.39IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FSW33.5e-22689.80IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
A0A6J1JE046.6e-22589.57IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111483601... [more]
A0A5A7ST021.5e-21685.49IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CVM41.2e-21585.27IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KQC32.1e-21584.82M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 P... [more]
Match NameE-valueIdentityDescription
AT1G51760.11.1e-13155.98peptidase M20/M25/M40 family protein [more]
AT5G56660.13.7e-12756.69IAA-leucine resistant (ILR)-like 2 [more]
AT1G51780.11.5e-12555.39IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56650.13.8e-12455.97IAA-leucine resistant (ILR)-like 1 [more]
AT1G44350.12.3e-12153.67IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.360coord: 224..342
e-value: 6.0E-143
score: 478.0
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 61..419
e-value: 6.0E-143
score: 478.0
NoneNo IPR availablePANTHERPTHR11014:SF147IAA-AMINO ACID HYDROLASE ILR1-LIKE 2coord: 7..441
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 56..436
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 225..323
e-value: 1.3E-10
score: 41.1
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 60..422
e-value: 5.5E-121
score: 402.1
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 45..438
e-value: 2.5E-130
score: 432.8
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 7..441
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 116..430
e-value: 1.2E-34
score: 120.1
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 59..434
e-value: 0.0
score: 662.477
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 224..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040034.1Lag0040034.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity