Lag0039975 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039975
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectin lyase-like superfamily protein
Location	chr13: 1260018 .. 1262986 (-)
RNA-Seq Expression	Lag0039975
Synteny	Lag0039975

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCAAAAGAAGCAAGAACTTAACTCTTTCGTTGTTTCTGTTTTTGGTGGTGTCTTCTTCTGTAGTTACAGTGGAGGCTAGGAAGCAACATCACAACAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGTTGGCAATGGCAATGGTTCAGGCCCTGCAACAGCTCCACTTCCTGAATACAATCAAACTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGTAAAAATTGTCCAAAACAACCTCAGAAAACAGATACAGAGATAGAGAAAAAGAGAGAGTTAAAAATGGTGACTGATGTACTGTTTTGAACTTGTAAAATCCATCCTATGCAGGCACTTCTAGCAGCATGGAAAGCTGCTTGCAAGATTCCTGGTGCTACATTGCAAATACCTTCACAATACAAGTTTCTCATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAACTTGTTCTTCAGGTTTGACTTATTTTCTTCTCCTTCAGGAATATGGTTTACTTGAAAATGTAATGAGACAAAGTATATTCTTTGACTTATCATGTAACAGATTGATTTTTTATAATAAATAAGTTAGTGCTGCTTAGAGTTCAAAAGATAAGATAATTAATGGAAAGTGCAATTCCTAAACAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTGAGTTCGTGGCCCAAATCCAGCTTGTTTCAGTGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGACAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAGGCTAGTTCTTTTTTTTTTAATAATCAACTCTTGTCGTCGTCATCATGTTATGCTAAGAGAGTGATTTTAGCTTAACCATTTCTTGTTTTTCCTGTTTCTGCAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGTAAACTTTTCTCACTCTTTCTCTCTTTCTTTTTGTTTAGACTTACAGCTTTTTTTTCTGAATTTTAATTGGGATTTTTTTTTAATACTTTGTTTATAAATGACAGGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGTATGAACCGAGACAATTTACAGCCCCTCGTTTATTATACTTTATTGTCCTTTCAAAAGTTAACTTATTCACTGGATCTTTCTCTGATCTGTTTTATAATTTTGAGGACACAATAAAGTGGTTTTTCAAAAAAGAAAATTTTGACTCAAATTTCAAAATACTTTTAAAACAAATGTTTTATTCTTGTTCACGTATCCTTTCTCTCAGATGTCTAATGATGTAGAACTATTTATCATATTGTAACTACCTATTCAAAATTCTCTCTACCATTAAACCAATGTTGTGGGGGTAAAAATAACTCCATAGGGGTTAAAAAAGTATCTCCATTTAAGAATATTTTGATTGTAAAAGCACATAAAGCTACACCACAAAGAAACTGGATTACACATTTGAACCAACAAGTAAAGAGAGGTGAATTACATAACTTATTTTAAGATGTATTATTTTGACAGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGGTAAATAAATCCTCATCAATTTTACTTTTTCTTAAAGGCAATTCCTGTAGAGAACATAATTTATGGCAGTGATTATAAATTAACCATTTTGCTCAACTTTTCTTAAACATTATTATATTCAGCACTAAAGATTATTTTCTTGCCTATAATTACAGCTTGGGCGGACTTGGGAAAGATAAAAGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATTTCAATTCAAAACACTCTTTCAGGAGTGAGGATCAAGACCTGGCAGGTAAAAAAGACAAGAAGAAAAGTTAAAACTACAAATCTAATCCTTGAATTATGGTGTCTATGTTAATTACTTCTTAAAAACTTTTAAACATTTCAACTGCATATCAACTCCGAATCATTGTCTATATATATTTTTTTTCTTTTTAACACCTTTAACAAAATGATGATTATAACATAGGTATATTGTATAAGTATGAATTAGTTTGACCAGAAGGAAGTAAACAGATAGGCAAAATAAAGCGACACGATTTATAAAAGAGATTTTCTATGTGAGCTCAAAAAAAGAGAGTATTATATAGTCCACATGCTTCTATCCAACCATCCACCTTCTTTTAGAGATTAAACAAACACTAGAAGAAACTTCAGGATTAAAGTTTGAAATCTATCGTAAAAAACCAACAATCTTATCCCATTTGGCCAATAAAAATTGTCATGATATAATCATACGGTTCTGATTGGATTTGATGGATTTGAACAGGGAGGAGTCGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCCATCATGATAGACCAATACTACTGCGACAAGTACTCATGCAAGAACCAAACAGGAGCAGTTGCAATCTCAGGTATAAAATACGATCAAATCATAGGAAGTTACACTGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAATCCCTTGCATAGACGTTGATCTCATTGACATCCAGCTGAATCCATCTTCAGAATCCAGAGGCTTTCAAAGCTCTCTGTGCTGGAACTCTTATGGCAAATCTCAAGCCCCCCTCGTTCCTTCAAGTATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGGAGAATTGCAAGGTCCCATGATCATGTCTGTTACTGA

mRNA sequence

ATGACCAAAAGAAGCAAGAACTTAACTCTTTCGTTGTTTCTGTTTTTGGTGGTGTCTTCTTCTGTAGTTACAGTGGAGGCTAGGAAGCAACATCACAACAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGTTGGCAATGGCAATGGTTCAGGCCCTGCAACAGCTCCACTTCCTGAATACAATCAAACTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGCACTTCTAGCAGCATGGAAAGCTGCTTGCAAGATTCCTGGTGCTACATTGCAAATACCTTCACAATACAAGTTTCTCATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAACTTGTTCTTCAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTGAGTTCGTGGCCCAAATCCAGCTTGTTTCAGTGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGACAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGCGGACTTGGGAAAGATAAAAGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATTTCAATTCAAAACACTCTTTCAGGAGTGAGGATCAAGACCTGGCAGGGAGGAGTCGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCCATCATGATAGACCAATACTACTGCGACAAGTACTCATGCAAGAACCAAACAGGAGCAGTTGCAATCTCAGGTATAAAATACGATCAAATCATAGGAAGTTACACTGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAATCCCTTGCATAGACGTTGATCTCATTGACATCCAGCTGAATCCATCTTCAGAATCCAGAGGCTTTCAAAGCTCTCTGTGCTGGAACTCTTATGGCAAATCTCAAGCCCCCCTCGTTCCTTCAAGTATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGGAGAATTGCAAGGTCCCATGATCATGTCTGTTACTGA

Coding sequence (CDS)

ATGACCAAAAGAAGCAAGAACTTAACTCTTTCGTTGTTTCTGTTTTTGGTGGTGTCTTCTTCTGTAGTTACAGTGGAGGCTAGGAAGCAACATCACAACAAGAAGGGCAAGCATCAGAAGCAAAAGGGTGTTGGCAATGGCAATGGTTCAGGCCCTGCAACAGCTCCACTTCCTGAATACAATCAAACTCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGCAATGGAGTTTCTGATGATTCCAAGGCACTTCTAGCAGCATGGAAAGCTGCTTGCAAGATTCCTGGTGCTACATTGCAAATACCTTCACAATACAAGTTTCTCATCAAACCCATAACTCTAAATGGCCCTTGTATGCCTCAACTTGTTCTTCAGATTGATGGAGCTGTATTGGCGCCTCCAAAAGTGAGTTCGTGGCCCAAATCCAGCTTGTTTCAGTGGCTGAATTTCAAATGGCTACATAACTTCACCATTCAAGGCTCTGGTACTGTGGATGGACAGGGCTTTAATTGGTGGACTCTCTCCACATTCTACAATAACAAGAAGAAATTGAAGCACATTCCAGACATGAAGCCTACTGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAATAAACAGTCCACAGTGCCATCTGAAATTTGACAACTCCGGCACCGTCAAAGTTGACAATATCACCATTTCTTCGCCGGAGAACAGCCCCAACACCGACGGCATTCACCTGCAGAACACACAAGATGTCGAAATTCAACACTCCAACATTGGCTGTGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCACATTCATCATATCAATTGTGGCCCTGGACATGGCATAAGCTTGGGCGGACTTGGGAAAGATAAAAGTGTTGCATGTGTCTCCAATATTGTTGTCGAAAACATTTCAATTCAAAACACTCTTTCAGGAGTGAGGATCAAGACCTGGCAGGGAGGAGTCGGGTCAGTGAAGAATGTATCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTGCCCATCATGATAGACCAATACTACTGCGACAAGTACTCATGCAAGAACCAAACAGGAGCAGTTGCAATCTCAGGTATAAAATACGATCAAATCATAGGAAGTTACACTGTGCAGCCTATTCACTTAGCCTGCAGCAACCAAATCCCTTGCATAGACGTTGATCTCATTGACATCCAGCTGAATCCATCTTCAGAATCCAGAGGCTTTCAAAGCTCTCTGTGCTGGAACTCTTATGGCAAATCTCAAGCCCCCCTCGTTCCTTCAAGTATAGACTACTGCTTGAGAAGGGGCAGTGGCGCTGTTAGGAGAATTGCAAGGTCCCATGATCATGTCTGTTACTGA

Protein sequence

MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEYNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGPCMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY

Homology

[Close]

BLAST of Lag0039975 vs. NCBI nr
Match: XP_022966725.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 867.8 bits (2241), Expect = 4.1e-248
Identity = 424/463 (91.58%), Postives = 436/463 (94.17%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAW AACKIPGATLQIP  YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNITISSPE
Sbjct: 181 YNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300

Query: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360
           KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD
Sbjct: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360

Query: 361 KYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQ 420
           KY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGFQ
Sbjct: 361 KYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQ 420

Query: 421 SSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           S+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 SALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

[Close]

BLAST of Lag0039975 vs. NCBI nr
Match: XP_023542214.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 866.3 bits (2237), Expect = 1.2e-247
Identity = 424/463 (91.58%), Postives = 435/463 (93.95%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQKG   G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKVDNITISSPE
Sbjct: 181 YNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGKD
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKD 300

Query: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360
           KS ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD
Sbjct: 301 KSAACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360

Query: 361 KYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQ 420
           KY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGFQ
Sbjct: 361 KYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQ 420

Query: 421 SSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           S+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 SALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

[Close]

BLAST of Lag0039975 vs. NCBI nr
Match: XP_022944899.1 (polygalacturonase At1g48100 isoform X4 [Cucurbita moschata])

HSP 1 Score: 865.5 bits (2235), Expect = 2.0e-247
Identity = 424/463 (91.58%), Postives = 436/463 (94.17%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITISSPE
Sbjct: 181 YNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGKD
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKD 300

Query: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360
           KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD
Sbjct: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360

Query: 361 KYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQ 420
           KY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGFQ
Sbjct: 361 KYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQ 420

Query: 421 SSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           S+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 SALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

[Close]

BLAST of Lag0039975 vs. NCBI nr
Match: XP_023542213.1 (polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 861.7 bits (2225), Expect = 2.9e-246
Identity = 424/464 (91.38%), Postives = 435/464 (93.75%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQKG   G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVL-QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLST 180
           CMPQLVL QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT+ST
Sbjct: 121 CMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVST 180

Query: 181 FYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSP 240
            YNNKKK KH+PDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKVDNITISSP
Sbjct: 181 LYNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITISSP 240

Query: 241 ENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGK 300
           E+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGK
Sbjct: 241 ESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGK 300

Query: 301 DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360
           DKS ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC
Sbjct: 301 DKSAACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360

Query: 361 DKYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGF 420
           DKY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGF
Sbjct: 361 DKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGF 420

Query: 421 QSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           QS+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 QSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

[Close]

BLAST of Lag0039975 vs. NCBI nr
Match: XP_022944898.1 (polygalacturonase At1g48100 isoform X3 [Cucurbita moschata])

HSP 1 Score: 860.9 bits (2223), Expect = 5.0e-246
Identity = 424/464 (91.38%), Postives = 436/464 (93.97%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVL-QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLST 180
           CMPQLVL QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT+ST
Sbjct: 121 CMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVST 180

Query: 181 FYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSP 240
            YNNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITISSP
Sbjct: 181 LYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSP 240

Query: 241 ENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGK 300
           E+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGK
Sbjct: 241 ESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGK 300

Query: 301 DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360
           DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC
Sbjct: 301 DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360

Query: 361 DKYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGF 420
           DKY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGF
Sbjct: 361 DKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGF 420

Query: 421 QSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           QS+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 QSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

[Close]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.2e-90
Identity = 190/465 (40.86%), Postives = 260/465 (55.91%), Query Frame = 0


Query: 10  LSLFLFLVVSSSVV----TVEARKQHHNKKGKH-------------QKQKGVGNGNGSGP 69
           LS  L + + S+V+    T  AR ++H  KG+H              +     +   S P
Sbjct: 4   LSGILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDP 63

Query: 70  ATAPLPEYNQTQS-TIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFL 129
             +P P    + S  +F++ SFGA G+G  DD+ A   AWKAAC +    +  P    F 
Sbjct: 64  YPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFK 123

Query: 130 IKPITLNGPCMPQLVLQIDGAVLAPPKVSSWP-KSSLFQWLNFKWLHNFTIQGSGTVDGQ 189
           I     +GPC P LV Q+DG ++ P     WP K +  QWL F  L  FT  G GTV+G 
Sbjct: 124 ITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGN 183

Query: 190 GFNWWTL--STFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGT 249
           G  WW L               P   PT +RF+ S N+ V+ + I NSPQ H+KFD    
Sbjct: 184 GQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQG 243

Query: 250 VKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGP 309
           V ++ I ISSP+ SPNTDGIHL NT+ V I +S +  GDDC+SI TGCS++ I  + CGP
Sbjct: 244 VLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGP 303

Query: 310 GHGISLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDV 369
            HGIS+G LG   S ACVSNI V N  I+++ +G+R+KTWQGG GSV N+ F NIQ+ +V
Sbjct: 304 SHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENV 363

Query: 370 KVPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSYTVQ--PIHLACSNQIPCIDVDLI 429
              I++DQYYC    C+N+T AV +  ++Y  I G+Y V+  PIH ACS+ + C ++ + 
Sbjct: 364 LNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMS 423

Query: 430 DIQLNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAV 452
           +++L P  E        CWN+YGK +   +P  ID CL  GS  V
Sbjct: 424 EVELLP-EEGELVDDPFCWNAYGKQETLTIP-PID-CLLDGSPVV 465

[Close]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.8e-81
Identity = 154/374 (41.18%), Postives = 224/374 (59.89%), Query Frame = 0


Query: 67  IFNILSFGAKGNGVSDDSKALLAAWKAACKIPGAT-LQIPSQYKFLIKPITLNGPCMPQL 126
           + ++ +FGAKGNGV+DD+KA   AWK AC     T + +P  Y  L++PI L+GPC  +L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 127 VLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNKK 186
            LQI G ++AP     W   +  +WL F  L   T++G GTV+G G  WW  S  +N+  
Sbjct: 112 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 171

Query: 187 KLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNT 246
             +      PTAL F+   N+ V ++ +I+S Q H+   +   V +  + + +P  SPNT
Sbjct: 172 PCRG----APTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNT 231

Query: 247 DGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVAC 306
           DGIH+  ++ + I ++ +  GDDC+SI    + I I +I CGPGHGIS+G LGK KS   
Sbjct: 232 DGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEE 291

Query: 307 VSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD-KYSC 366
           V +I V+   I +T +GVRIKTWQGG G V  + F NI++++V  PI+IDQYYCD +  C
Sbjct: 292 VRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPC 351

Query: 367 KNQTGAVAISGIKYDQIIG-SYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSL 426
            NQT A++I  I +  + G S + + I ++CS+  PC ++ L DI L PS+   GF  S 
Sbjct: 352 ANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSN-GDGFTESF 411

Query: 427 CWNSYGKSQAPLVP 438
           CW +YG S   + P
Sbjct: 412 CWEAYGSSSGQVYP 420

[Close]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-77
Identity = 155/396 (39.14%), Postives = 232/396 (58.59%), Query Frame = 0


Query: 67  IFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGPCMPQLV 126
           IFN+  +GA G+G  D ++A   AW+AACK P A L +P   KF++  +  NGPC P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 127 LQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNKKK 186
            ++DG + A    +SW  + +  WL F  L  FT+ G G +DGQG  WW     + N ++
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177

Query: 187 LKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTD 246
           + +  D +PTA++F  S  + ++ + ++NSP+ HL F N   VK+  I+I++P +SPNTD
Sbjct: 178 ICNDRD-RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTD 237

Query: 247 GIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACV 306
           GI +  +++  +Q + IG GDDCV+I TG SNI I  + CGPGHGIS+G LG++ S A V
Sbjct: 238 GIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEV 297

Query: 307 SNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYS-CK 366
           S + V      +T +G+RIKTWQGG G   ++ + N+++ + + PI+I+Q+YC   S C+
Sbjct: 298 SYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQ 357

Query: 367 NQTGAVAISGIKYDQIIG-SYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSLC 426
           NQ  AV I  + Y  I G S T   I L CS+ +PC D+ L DI L  +S      S L 
Sbjct: 358 NQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGK--IASCLN 417

Query: 427 WNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH 461
            N+ G     ++P+    C      A R+ ++SH H
Sbjct: 418 DNANGYFSGHVIPA----CKNLSPSAKRKESKSHKH 444

[Close]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.6e-75
Identity = 144/368 (39.13%), Postives = 217/368 (58.97%), Query Frame = 0


Query: 65  STIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGPCMPQ 124
           +T+FN+  +GA G+G  D ++A    W AACK   A L +P+  KF +  +   GPC P 
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115

Query: 125 LVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNK 184
           L  ++DG ++A P  + W  S +  WL F  L +F + G+G +DGQG  WW       N 
Sbjct: 116 LSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNG 175

Query: 185 KKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPN 244
           + + +  + +PTA++   S +VTV+++T++NSP+ HL F     VK+  + I +P +SPN
Sbjct: 176 RTVCNDRN-RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPN 235

Query: 245 TDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVA 304
           TDGI +  ++   I+   IG GDDC++I TG SNI I  + CGPGHGIS+G LG+D S A
Sbjct: 236 TDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRA 295

Query: 305 CVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYS- 364
            VS++ V      +T +G+RIKTWQGG G    +++ N+++ + + PI+I+Q+YC   S 
Sbjct: 296 EVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASA 355

Query: 365 CKNQTGAVAISGIKYDQIIG-SYTVQPIHLACSNQIPCIDVDLIDIQLNPSS-------- 422
           C+NQ  AV I G+ Y  I G S T   I L CS+ +PC  + L ++ L  +S        
Sbjct: 356 CQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVD 415

[Close]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 3.1e-73
Identity = 146/351 (41.60%), Postives = 213/351 (60.68%), Query Frame = 0


Query: 66  TIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGA-TLQIPSQYKFLIKPITLNGPCMPQ 125
           T  ++  FGAKG+G +DD++A + AWK AC   GA  L +P    +L+K I L GPC   
Sbjct: 66  TTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 125

Query: 126 LVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQG--SGTVDGQGFNWWTLSTFYN 185
           L +QI G + A  K S +    + +W+ F  ++N ++ G  +G VDG G  WW      N
Sbjct: 126 LTVQIFGTLSASQKRSDY--KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWW-----QN 185

Query: 186 NKKKLKHIPDMK-PTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPEN 245
           + K+ K  P  K PTAL FY+S ++ V+++ + N+ Q  +  +    V+V N+ +++P +
Sbjct: 186 SCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPAD 245

Query: 246 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 305
           SPNTDGIH+ NTQ++ +  S IG GDDC+SI++G  N+ I+ I CGPGHGIS+G LG D 
Sbjct: 246 SPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDN 305

Query: 306 SVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDK 365
           S A VS + V+   +  T +GVRIKT+QGG G+  N+ F NIQ+ +VK PI+IDQ YCDK
Sbjct: 306 SKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDK 365

Query: 366 YSCKNQTGAVAISGIKYDQIIG-SYTVQPIHLACSNQIPC--IDVDLIDIQ 410
             C  +  AV +  + Y  I G S +   I   CS   PC  I +D ++I+
Sbjct: 366 SKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK 409

[Close]

BLAST of Lag0039975 vs. ExPASy TrEMBL
Match: A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.0e-248
Identity = 424/463 (91.58%), Postives = 436/463 (94.17%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAW AACKIPGATLQIP  YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI GSGTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KH+PDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSG VKVDNITISSPE
Sbjct: 181 YNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300

Query: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360
           KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD
Sbjct: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360

Query: 361 KYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQ 420
           KY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGFQ
Sbjct: 361 KYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQ 420

Query: 421 SSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           S+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 SALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

[Close]

BLAST of Lag0039975 vs. ExPASy TrEMBL
Match: A0A6J1FZF6 (polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.8e-248
Identity = 424/463 (91.58%), Postives = 436/463 (94.17%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITISSPE
Sbjct: 181 YNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKD 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGKD
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKD 300

Query: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360
           KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD
Sbjct: 301 KSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCD 360

Query: 361 KYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQ 420
           KY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGFQ
Sbjct: 361 KYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQ 420

Query: 421 SSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           S+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 SALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 462

[Close]

BLAST of Lag0039975 vs. ExPASy TrEMBL
Match: A0A6J1FZB5 (polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.4e-246
Identity = 424/464 (91.38%), Postives = 436/464 (93.97%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVL-QIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLST 180
           CMPQLVL QIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT+ST
Sbjct: 121 CMPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVST 180

Query: 181 FYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSP 240
            YNNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITISSP
Sbjct: 181 LYNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSP 240

Query: 241 ENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGK 300
           E+SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGISLGGLGK
Sbjct: 241 ESSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGK 300

Query: 301 DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360
           DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC
Sbjct: 301 DKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYC 360

Query: 361 DKYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGF 420
           DKY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQLNPSS SRGF
Sbjct: 361 DKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGF 420

Query: 421 QSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           QS+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 QSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 463

[Close]

BLAST of Lag0039975 vs. ExPASy TrEMBL
Match: A0A1S3BEF8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 413/448 (92.19%), Postives = 433/448 (96.65%), Query Frame = 0


Query: 16  LVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEYNQTQSTIFNILSFGA 75
           L+VSSSVV VEARKQ+H KK KHQKQKG GNG+GSGPA APLP YNQ+QSTIFNILSFGA
Sbjct: 4   LLVSSSVVRVEARKQYH-KKSKHQKQKGDGNGHGSGPAPAPLPYYNQSQSTIFNILSFGA 63

Query: 76  KGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGPCMPQLVLQIDGAVLA 135
           KGNGVSDDSKALLAAWKAAC+IPGATL+IPSQ+KFLIKPITLNGPCM QLVLQIDGAVLA
Sbjct: 64  KGNGVSDDSKALLAAWKAACEIPGATLEIPSQHKFLIKPITLNGPCMAQLVLQIDGAVLA 123

Query: 136 PPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNKKKLKHIPDMKP 195
           PPKVSSWPKSSLFQWLNFKWLHNFT+QGSGTV+GQGFNWWTLSTFYNNKKKLKHIPDMKP
Sbjct: 124 PPKVSSWPKSSLFQWLNFKWLHNFTVQGSGTVNGQGFNWWTLSTFYNNKKKLKHIPDMKP 183

Query: 196 TALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQD 255
           TALRFYSSYNVTVRDITIINSPQCHLKFDNSG VK+DNITISSPENSPNTDGIHLQNT+D
Sbjct: 184 TALRFYSSYNVTVRDITIINSPQCHLKFDNSGAVKIDNITISSPENSPNTDGIHLQNTRD 243

Query: 256 VEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVSNIVVENIS 315
           VEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKS ACVSNIVV+NIS
Sbjct: 244 VEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSAACVSNIVVQNIS 303

Query: 316 IQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYSCKNQTGAVAISG 375
           IQNTLSGVRIKTWQGG+GSVKNVSFSNIQVSDVKVPIMIDQYYCDK  CKNQTGAVAISG
Sbjct: 304 IQNTLSGVRIKTWQGGIGSVKNVSFSNIQVSDVKVPIMIDQYYCDKSKCKNQTGAVAISG 363

Query: 376 IKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSLCWNSYGKSQAPL 435
           I YDQIIGSY+VQPIHLACS+QIPC+DVDLIDIQL PSSESRGFQ+SLCWNSYGKSQAPL
Sbjct: 364 ITYDQIIGSYSVQPIHLACSSQIPCVDVDLIDIQLKPSSESRGFQNSLCWNSYGKSQAPL 423

Query: 436 VPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           +P+SID CLRRG GAVRRIARSHD+VCY
Sbjct: 424 LPTSIDSCLRRGGGAVRRIARSHDYVCY 450

[Close]

BLAST of Lag0039975 vs. ExPASy TrEMBL
Match: A0A6J1FZF8 (polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.5e-245
Identity = 424/474 (89.45%), Postives = 436/474 (91.98%), Query Frame = 0


Query: 1   MTKRSKNLTLSLFLFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEY 60
           M K SK LTL L + L+VSSSVV +EARK HHNKK KHQKQ+G G G+GS PA APLP+Y
Sbjct: 1   MNKGSKILTLFL-VSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDY 60

Query: 61  NQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGP 120
           NQTQSTIFNILSFGAKGNGV DDSKAL+AAWKAACKIPGATLQIPS YKFLIKPITLNGP
Sbjct: 61  NQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP 120

Query: 121 CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTF 180
           CMPQLVLQIDG VLAPPKVSSW KSSLFQWLNFKWLHNFTI G GTVDGQGFNWWT+ST 
Sbjct: 121 CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTL 180

Query: 181 YNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPE 240
           YNNKKK KHIPDMKPTALRFYSSYNVTVRDITI NSPQCHLKFDNSGTVKV+NITISSPE
Sbjct: 181 YNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPE 240

Query: 241 NSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGI-------- 300
           +SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHI CGPGHGI        
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTRGLFLA 300

Query: 301 ---SLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVK 360
              SLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVK
Sbjct: 301 YNYSLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVK 360

Query: 361 VPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQ 420
           VPIMIDQYYCDKY CKNQTGAVAISGIKYDQIIGSYTVQPIHLACS+QIPCIDVDLI IQ
Sbjct: 361 VPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQ 420

Query: 421 LNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHVCY 464
           LNPSS SRGFQS+LCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDH CY
Sbjct: 421 LNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 473

[Close]

BLAST of Lag0039975 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 9.6e-179
Identity = 299/457 (65.43%), Postives = 370/457 (80.96%), Query Frame = 0


Query: 14  LFLVVSSSVVTVEARKQHHNKKGKHQKQKGVGNGNGSGPATAPLPEYNQTQSTIFNILSF 73
           + +  + S V+VE RK HH KK K + ++   N     PA AP P  N+    +FNI S+
Sbjct: 17  IIVAAAISTVSVEGRK-HHVKKIKPKHRRHSKNTPTGSPAPAPYPSTNE---GVFNIFSY 76

Query: 74  GAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNGPCMPQLVLQIDGAV 133
           GAKG+GVSDDSKAL+ AWKAACK+ G  ++IP+  +FL+K +TL GPC  + V+QI+G +
Sbjct: 77  GAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPCKEETVVQIEGIL 136

Query: 134 LAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNNKKKLKHIPDM 193
           +AP K+ SWP SSLFQWLNFKW+ + TIQGSGT++G+G+NWW L T Y  + + K+IP M
Sbjct: 137 VAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDT-YQTQTRNKYIPPM 196

Query: 194 KPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNT 253
           KPTALRFYSS NVTVRDI+I+NSP CHLKFD+S  VKV+NITISSPENSPNTDGIHLQNT
Sbjct: 197 KPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENSPNTDGIHLQNT 256

Query: 254 QDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVSNIVVEN 313
           ++VEIQHSNI CGDDCVSIQTG SN+HIHHINCGPGHGIS+GGLGKDKSVACVS+I+VE+
Sbjct: 257 RNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKSVACVSDIIVED 316

Query: 314 ISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYSCKNQTGAVAI 373
           ISIQNTL+GVRIKTWQGG+G VKN++FSNIQV DVKVPI+IDQYYCDK  CKNQT AV+I
Sbjct: 317 ISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKSKCKNQTRAVSI 376

Query: 374 SGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQS-----SLCWNSY 433
           SG+KY+ I+GS+TVQP+ +ACSN +PC+DVDL+DI+L PS   RG Q+     +LCWNSY
Sbjct: 377 SGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTHQQQQALCWNSY 436

Query: 434 GKSQAPLVPSSIDYCLRR---GSGAVRRIARSHDHVC 463
           GK+Q PLVPSSI YCLR+   G    ++++RS+D +C
Sbjct: 437 GKTQGPLVPSSIGYCLRKSNIGGYYSQKVSRSYDKIC 468

[Close]

BLAST of Lag0039975 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 1.4e-121
Identity = 209/434 (48.16%), Postives = 284/434 (65.44%), Query Frame = 0


Query: 52  PATAPLPEYNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFL 111
           P   P P   Q  S +FN++ FGAKG+G  DD+KA  AAW AACKI  + + +P +Y +L
Sbjct: 103 PKVQPPPLPPQKGSQVFNVMDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYL 162

Query: 112 IKPITLNGP-CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQ 171
           + PI+ +GP C   +V Q+DG ++AP    +W K  L  W++F  L    +QG G +DG+
Sbjct: 163 VGPISFSGPYCQANIVFQLDGTIIAPTDSKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGR 222

Query: 172 GFNWWTL-STFYNNKKKL---------------------KHIPDMKPTALRFYSSYNVTV 231
           G  WW   S F ++  KL                     + +P +KPTALRF  S+ V V
Sbjct: 223 GSGWWQQDSPFIDSDTKLIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEV 282

Query: 232 RDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDD 291
             ITI NSPQCHLKFD+   V V +I +SSP +SPNTDGIHLQNT+DV I  + + CGDD
Sbjct: 283 TGITIQNSPQCHLKFDDCVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDD 342

Query: 292 CVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTW 351
           C+SIQTGCSN+ +H++NCGPGHGIS+G LGK+ + ACVSNI V ++++ NT++GVRIKTW
Sbjct: 343 CISIQTGCSNVFVHNVNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTW 402

Query: 352 QGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSYTVQ 411
           QGGVGSVK + FSNIQ++ V++PI I+Q+YCD   CKNQT AVA+ G+ Y++I G+YTV+
Sbjct: 403 QGGVGSVKGIIFSNIQLNQVQIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVK 462

Query: 412 PIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCLRRGS 463
           P+H ACS+  PC+DV L  I+L P  E      + CW ++G+   P +P  ID CL+ G 
Sbjct: 463 PVHFACSDNFPCVDVQLSSIELKPVQEKYRMYDAYCWQTFGELNTPTLP-PID-CLKIGK 522

[Close]

BLAST of Lag0039975 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 431.0 bits (1107), Expect = 1.2e-120
Identity = 211/434 (48.62%), Postives = 283/434 (65.21%), Query Frame = 0


Query: 52  PATAPLPEYNQTQSTIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFL 111
           P   PLP      S  FN+L FGAKG+G+SDD++A  AAW +ACK+  +T+ IP  Y FL
Sbjct: 107 PPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFL 166

Query: 112 IKPITLNGP-CMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQ 171
           + PI+ +GP C   +V Q++G ++AP    SW    L  W+ F  L   TIQG+G +DG+
Sbjct: 167 VGPISFSGPYCQANIVFQLEGMIVAPTDTESW-GGGLMWWIEFTKLSGITIQGNGVIDGR 226

Query: 172 GFNWWT---LSTF-----------YNNKKKLK-----------HIPDMKPTALRFYSSYN 231
           G  WW    LS +            NN  + +            +P +KPTALRFY S +
Sbjct: 227 GTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSID 286

Query: 232 VTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGC 291
           VTV  ITI NSPQCHLKFDN   V V ++ +SSP +SPNTDGIHLQNT+DV I  + + C
Sbjct: 287 VTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLAC 346

Query: 292 GDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSVACVSNIVVENISIQNTLSGVRI 351
           GDDC+SIQTGCSN+++H++NCGPGHGIS+G LGKD + ACVSNI V ++ + NT++GVRI
Sbjct: 347 GDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRI 406

Query: 352 KTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSY 411
           KTWQGG+GSVK + FSNIQ+++V++PI+IDQ+YCD   C N T AV++ G+ Y++I G+Y
Sbjct: 407 KTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTY 466

Query: 412 TVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCLR 460
           TV+P+H ACS+  PCIDV L  I+L P           CW ++G+  +  VP  ID CL+
Sbjct: 467 TVKPVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVP-PID-CLQ 526

[Close]

BLAST of Lag0039975 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 402.1 bits (1032), Expect = 5.9e-112
Identity = 193/374 (51.60%), Postives = 252/374 (67.38%), Query Frame = 0


Query: 65  STIFNILSFGAKGNGVSDDSKALLAAWKAACKIPGATLQIPSQYKFLIKPITLNG-PCMP 124
           S   N+L  GAKG+G SDD+KA   AW+ ACK+  +TL +PS   FL+ P++  G  C  
Sbjct: 48  SGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKE 107

Query: 125 QLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSGTVDGQGFNWWTLSTFYNN 184
           ++V Q++G ++AP   S+W  S L QW+ FK L   TI+G G +DG+G  WW       N
Sbjct: 108 KIVFQLEGKIIAPTSASAW-GSGLLQWIEFKALQGITIKGKGIIDGRGSVWW-------N 167

Query: 185 KKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGTVKVDNITISSPENSP 244
                 +P  KPTALRFY S  VTV  ITI NSPQ HLKFDN  +++V + T SSP +SP
Sbjct: 168 DMMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSP 227

Query: 245 NTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDKSV 304
           NTDGIHLQN+QD  I  S + CGDDC+SIQTGCSNI+IH ++CGPGHGIS+GGLGKD + 
Sbjct: 228 NTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTK 287

Query: 305 ACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNIQVSDVKVPIMIDQYYCDKYS 364
           ACVSNI V ++++  T +GVRIK+WQGG GSVK V FSNIQVS+V  PI+IDQYYCD   
Sbjct: 288 ACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGG 347

Query: 365 CKNQTGAVAISGIKYDQIIGSYTVQPIHLACSNQIPCIDVDLIDIQLNPSSESRGFQSSL 424
           C N+T AVA+S I Y  I G+YT +P+  ACS+ +PC  + L  I+L P++         
Sbjct: 348 CHNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPF 407

Query: 425 CWNSYGKSQAPLVP 438
           CW ++G+ +   +P
Sbjct: 408 CWKAHGELKTKTLP 413

[Close]

BLAST of Lag0039975 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-97
Identity = 180/403 (44.67%), Postives = 250/403 (62.03%), Query Frame = 0


Query: 52  PATAPLPEYNQTQS---TIFNILSFGAKGNGVSDDSKALLAAWKAACKIPG---ATLQIP 111
           P+ AP P YN   +   T+F++ SFGA G+  +DD+ A   AW AAC   G   A L +P
Sbjct: 29  PSPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVP 88

Query: 112 SQYKFLIKPITLNGPCMPQLVLQIDGAVLAPPKVSSWPKSSLFQWLNFKWLHNFTIQGSG 171
             + FL+KP T NGPC   LVLQIDG +++P    SWP +   QW+ F  ++  +IQGSG
Sbjct: 89  YTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSG 148

Query: 172 TVDGQGFNWWTL--STFYNNKKKLKHIPDMKPTALRFYSSYNVTVRDITIINSPQCHLKF 231
            ++G+G  WW L            +  P   P A+R + S  V ++ I  +NS Q H++F
Sbjct: 149 VINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRF 208

Query: 232 DNSGTVKVDNITISSPENSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHH 291
           DN   V VD++ I +P +SPNTDGIH++NT +V+I++S I  GDDC+SI  GC N+ I +
Sbjct: 209 DNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIKN 268

Query: 292 INCGPGHGISLGGLGKDKSVACVSNIVVENISIQNTLSGVRIKTWQGGVGSVKNVSFSNI 351
           + CGP HGIS+G LG   S A VSNI V N +I N+ +GVRIKTWQGG GSV  + FSNI
Sbjct: 269 VTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNI 328

Query: 352 QVSDVKVPIMIDQYYCDKYSCKNQTGAVAISGIKYDQIIGSYTVQ--PIHLACSNQIPCI 411
            + +V+ PIMIDQYYC   +C NQT AV IS + Y  I G+Y ++  PIH  CS+ +PC 
Sbjct: 329 LMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPCT 388

Query: 412 DVDLIDIQLNPSSESRGFQSSLCWNSYGKSQAPLVPSSIDYCL 445
           ++ L ++ L P S+ +  ++  CWN+YG  +  + P    YCL
Sbjct: 389 NLTLTEVDLFP-SKGQHLENPFCWNAYGSMKITVPPV---YCL 427

The following BLAST results are available for this feature:

BLAST of Lag0039975 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022966725.1	4.1e-248	91.58	polygalacturonase At1g48100 [Cucurbita maxima]	[more]
XP_023542214.1	1.2e-247	91.58	polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022944899.1	2.0e-247	91.58	polygalacturonase At1g48100 isoform X4 [Cucurbita moschata]	[more]
XP_023542213.1	2.9e-246	91.38	polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022944898.1	5.0e-246	91.38	polygalacturonase At1g48100 isoform X3 [Cucurbita moschata]	[more]

BLAST of Lag0039975 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.2e-90	40.86	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	1.8e-81	41.18	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	1.6e-77	39.14	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	5.6e-75	39.13	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q8RY29	3.1e-73	41.60	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of Lag0039975 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HSE8	2.0e-248	91.58	polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=...	[more]
A0A6J1FZF6	9.8e-248	91.58	polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1FZB5	2.4e-246	91.38	polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A1S3BEF8	1.2e-245	92.19	polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103488989 PE=3 SV=1	[more]
A0A6J1FZF8	3.5e-245	89.45	polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of Lag0039975 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26610.1	9.6e-179	65.43	Pectin lyase-like superfamily protein	[more]
AT1G10640.1	1.4e-121	48.16	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	1.2e-120	48.62	Pectin lyase-like superfamily protein	[more]
AT5G14650.1	5.9e-112	51.60	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	1.8e-97	44.67	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 306..327 e-value: 15.0 score: 14.5 coord: 276..296 e-value: 7800.0 score: 0.9 coord: 203..252 e-value: 46.0 score: 12.9 coord: 335..356 e-value: 2400.0 score: 4.5 coord: 253..274 e-value: 4000.0 score: 2.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 51..445 e-value: 4.5E-116 score: 390.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 100..414 e-value: 7.1E-81 score: 271.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..56
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 24..451
None	No IPR available	PANTHER	PTHR31375:SF92	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 24..451
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 61..440

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039975.1	Lag0039975.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity