Lag0039580 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0039580
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Protein DETOXIFICATION
Location	chr2: 46802078 .. 46803108 (+)
RNA-Seq Expression	Lag0039580
Synteny	Lag0039580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTGAGTGTGCCTCTCTTTGGGTGTCACAATATTTTGGGGTATGCATTCACAAGTGATAAACAAATTGCCAATCACATTGCTTCTATGTGGCCTTTCATTTGCCTTTCCATTCTCAATGATAGTTTTCTTGGTATCCTTTCAGGTATGTATCTCAATATCTTTAAATTCTAAATTTTCAAACCTCTTATAGTTTCACTAAAAAAATTTTATGCACAAATTGGTTTATATAGTTTAAGTTTAGGTTTAATTTCATTCTTATATTCTTAAAATTTTCAATTGGATTCTTTTGGTTTGGTTTCAAATTAGAAAATTGTTCTTATTGTTATAATTAAGTTGATAGCCTGGTGAATTGTCTATATGTTTCATAATTATGTGGCAATACACTAATTCGTTCGATTTGGTTAACTATGTTTAGTTAAATAATGTTTAAGAGTTTATGACTAAATGTATATATGTATGATAATTTAGAATGTGTGGGGACATTTTAGGGGTTGCAAGAGGCACTGGATGGCAACGGTTAGGTGCATATGTGAATTTTGGATCATATTATATAGTTGGGACTCCAATGGCAGTTGTGTTATGTTTTGTGGCACATTTGAGAATCAAAGGACTTTGGATTGGCTTGGTTTCAGGAGCAGCACTTCAAGGCTTTCTTTTTGCTCTCATCACTGCTTCAACTAATTGGCATAAACAGGTTCACTCTCTCTTCACATTTTTTCAATTAAGTGTTTTCCAAGTACTAATTATAATATTTTATAATTTTTTTATGTCAATGTTGTTAATAACATTTTGACTACATAGAAAAATTATTCACAATTTGAGAGTGTAGTGGAAACTTTTTAATGTCTGGGAACTAAGTCACAGGTATGATGCTAAAGTATATATAACAACTAATTTTGAAAGGTTAAATTATTAAAAATGTTAAGGATATGAGTCACTCGTGCCAGCTTGGCCTCAAGGAAAACACTCAGCCATACCATCTTGGATTCTCCAACACAGTCAGCATACCTACCTACGTTAGCTGA

mRNA sequence

ATGGTTGTGAGTGTGCCTCTCTTTGGGTGTCACAATATTTTGGGGTATGCATTCACAAGTGATAAACAAATTGCCAATCACATTGCTTCTATGTGGCCTTTCATTTGCCTTTCCATTCTCAATGATAGTTTTCTTGGTATCCTTTCAGGGGTTGCAAGAGGCACTGGATGGCAACGGTTAGGTGCATATGTGAATTTTGGATCATATTATATAGTTGGGACTCCAATGGCAGTTGTGTTATGTTTTGTGGCACATTTGAGAATCAAAGGACTTTGGATTGGCTTGGTTTCAGGAGCAGCACTTCAAGGCTTTCTTTTTGCTCTCATCACTGCTTCAACTAATTGGCATAAACAGGATATGAGTCACTCGTGCCAGCTTGGCCTCAAGGAAAACACTCAGCCATACCATCTTGGATTCTCCAACACAGTCAGCATACCTACCTACGTTAGCTGA

Coding sequence (CDS)

ATGGTTGTGAGTGTGCCTCTCTTTGGGTGTCACAATATTTTGGGGTATGCATTCACAAGTGATAAACAAATTGCCAATCACATTGCTTCTATGTGGCCTTTCATTTGCCTTTCCATTCTCAATGATAGTTTTCTTGGTATCCTTTCAGGGGTTGCAAGAGGCACTGGATGGCAACGGTTAGGTGCATATGTGAATTTTGGATCATATTATATAGTTGGGACTCCAATGGCAGTTGTGTTATGTTTTGTGGCACATTTGAGAATCAAAGGACTTTGGATTGGCTTGGTTTCAGGAGCAGCACTTCAAGGCTTTCTTTTTGCTCTCATCACTGCTTCAACTAATTGGCATAAACAGGATATGAGTCACTCGTGCCAGCTTGGCCTCAAGGAAAACACTCAGCCATACCATCTTGGATTCTCCAACACAGTCAGCATACCTACCTACGTTAGCTGA

Protein sequence

MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRLGAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDMSHSCQLGLKENTQPYHLGFSNTVSIPTYVS

Homology

[Close]

BLAST of Lag0039580 vs. NCBI nr
Match: KAG6592362.1 (Protein DETOXIFICATION 14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-49
Identity = 101/133 (75.94%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           + VS  LFGCHN+LGYAFTSD QIANHIAS WP ICLSIL DSFLGILSGVARG GWQR+
Sbjct: 328 VTVSAALFGCHNMLGYAFTSDTQIANHIASFWPLICLSILIDSFLGILSGVARGCGWQRV 387

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN GSYYIVG PMA VL FV HLR+KGLWIGLVSGA LQ FLFALIT  TNWHKQ +
Sbjct: 388 GAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWIGLVSGATLQSFLFALITTFTNWHKQAL 447

Query: 121 SHSCQLGLKENTQ 134
             + +  L+ENT+
Sbjct: 448 K-ARERVLEENTR 459

[Close]

BLAST of Lag0039580 vs. NCBI nr
Match: XP_022925516.1 (protein DETOXIFICATION 8-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-49
Identity = 101/133 (75.94%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           + VS  LFGCHN+LGYAFTSD QIANHIAS WP ICLSIL DSFLGILSGVARG GWQR+
Sbjct: 279 VTVSAALFGCHNMLGYAFTSDTQIANHIASFWPLICLSILIDSFLGILSGVARGCGWQRV 338

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN GSYYIVG PMA VL FV HLR+KGLWIGLVSGA LQ FLFALIT  TNWHKQ +
Sbjct: 339 GAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWIGLVSGATLQSFLFALITTFTNWHKQAL 398

Query: 121 SHSCQLGLKENTQ 134
             + +  L+ENT+
Sbjct: 399 K-ARERVLEENTR 410

[Close]

BLAST of Lag0039580 vs. NCBI nr
Match: XP_022925514.1 (protein DETOXIFICATION 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-49
Identity = 101/133 (75.94%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           + VS  LFGCHN+LGYAFTSD QIANHIAS WP ICLSIL DSFLGILSGVARG GWQR+
Sbjct: 354 VTVSAALFGCHNMLGYAFTSDTQIANHIASFWPLICLSILIDSFLGILSGVARGCGWQRV 413

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN GSYYIVG PMA VL FV HLR+KGLWIGLVSGA LQ FLFALIT  TNWHKQ +
Sbjct: 414 GAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWIGLVSGATLQSFLFALITTFTNWHKQAL 473

Query: 121 SHSCQLGLKENTQ 134
             + +  L+ENT+
Sbjct: 474 K-ARERVLEENTR 485

[Close]

BLAST of Lag0039580 vs. NCBI nr
Match: XP_022925515.1 (protein DETOXIFICATION 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 206.5 bits (524), Expect = 1.7e-49
Identity = 101/133 (75.94%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           + VS  LFGCHN+LGYAFTSD QIANHIAS WP ICLSIL DSFLGILSGVARG GWQR+
Sbjct: 329 VTVSAALFGCHNMLGYAFTSDTQIANHIASFWPLICLSILIDSFLGILSGVARGCGWQRV 388

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN GSYYIVG PMA VL FV HLR+KGLWIGLVSGA LQ FLFALIT  TNWHKQ +
Sbjct: 389 GAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWIGLVSGATLQSFLFALITTFTNWHKQAL 448

Query: 121 SHSCQLGLKENTQ 134
             + +  L+ENT+
Sbjct: 449 K-ARERVLEENTR 460

[Close]

BLAST of Lag0039580 vs. NCBI nr
Match: XP_022973955.1 (protein DETOXIFICATION 8-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 205.3 bits (521), Expect = 3.7e-49
Identity = 100/133 (75.19%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           + +S  LFGCHN+LGYAFTSD QIANHIAS WP ICLSIL DSFLGILSGVARG GWQR+
Sbjct: 279 VTMSAALFGCHNMLGYAFTSDTQIANHIASFWPLICLSILIDSFLGILSGVARGCGWQRV 338

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN GSYYIVG PMA VL FV HLR+KGLWIGLVSGA LQ FLFALIT  TNWHKQ +
Sbjct: 339 GAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWIGLVSGATLQSFLFALITTFTNWHKQAL 398

Query: 121 SHSCQLGLKENTQ 134
             + +  L+ENT+
Sbjct: 399 K-ARERVLEENTR 410

[Close]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot
Match: F4HQ05 (Protein DETOXIFICATION 8 OS=Arabidopsis thaliana OX=3702 GN=DTX8 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 4.7e-31
Identity = 59/117 (50.43%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 2   VVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRLG 61
           +VS+ L+       Y F+++ ++A+++  + PF+CLSI  DSFL +LSGVARGTGWQ +G
Sbjct: 352 IVSISLYSYRRNWAYIFSNESEVADYVTQITPFLCLSIGVDSFLAVLSGVARGTGWQHIG 411

Query: 62  AYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQ 119
           AY N GSYY+VG P+  +LCFV  LR KGLWIG++ G+ LQ  + AL+T  TNW ++
Sbjct: 412 AYANIGSYYLVGIPVGSILCFVVKLRGKGLWIGILVGSTLQTIVLALVTFFTNWEQE 468

[Close]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot
Match: Q9SIA5 (Protein DETOXIFICATION 1 OS=Arabidopsis thaliana OX=3702 GN=DTX1 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 54/115 (46.96%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 4   SVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRLGAY 63
           S+ LF C NI+GYAF++ K++ +++A + P +CLS + D F  +L+GVARG+GWQ +GA+
Sbjct: 346 SILLFTCRNIIGYAFSNSKEVLDYVADLTPLLCLSFILDGFTAVLNGVARGSGWQHIGAW 405

Query: 64  VNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQ 119
            N  SYY+VG P+ + L F   L  KGLW G+V G+ +Q  + A++TAS NW +Q
Sbjct: 406 NNTVSYYLVGAPVGIYLAFSRELNGKGLWCGVVVGSTVQATILAIVTASINWKEQ 460

[Close]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot
Match: Q9C9M8 (Protein DETOXIFICATION 9 OS=Arabidopsis thaliana OX=3702 GN=DTX9 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.2e-28
Identity = 54/127 (42.52%), Postives = 87/127 (68.50%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           ++VS  LF   ++  YA+++ +++ +++  + P +C+SIL DSFL +LSG+ RGTGWQ++
Sbjct: 344 VIVSSSLFLSRSVWPYAYSNVEEVISYVTDITPILCISILMDSFLTVLSGIVRGTGWQKI 403

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQDM 120
           GAYVN  SYY++G P+ ++LCF  H   KGLW GLV+G+ LQ  +  L+   TNW K+ +
Sbjct: 404 GAYVNITSYYVIGIPVGLLLCFHLHFNGKGLWAGLVTGSTLQTLILFLVIGFTNWSKEAI 463

Query: 121 SHSCQLG 128
               ++G
Sbjct: 464 KARERIG 470

[Close]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot
Match: Q9SIA4 (Protein DETOXIFICATION 3 OS=Arabidopsis thaliana OX=3702 GN=DTX3 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-28
Identity = 55/115 (47.83%), Postives = 80/115 (69.57%), Query Frame = 0


Query: 4   SVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRLGAY 63
           S  LF C NI+GYAF++ K++ +++A++ P +CLS + D F  +L+GVARG+GWQ +GA 
Sbjct: 346 STLLFTCRNIIGYAFSNSKEVVDYVANLTPLLCLSFILDGFTAVLNGVARGSGWQHIGAL 405

Query: 64  VNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQ 119
            N  +YY+VG P+ V L F   L  KGLW G+V G+A+Q  + A +TAS NW +Q
Sbjct: 406 NNVVAYYLVGAPVGVYLAFNRELNGKGLWCGVVVGSAVQAIILAFVTASINWKEQ 460

[Close]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot
Match: Q8VYL8 (Protein DETOXIFICATION 10 OS=Arabidopsis thaliana OX=3702 GN=DTX10 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.7e-28
Identity = 56/118 (47.46%), Postives = 81/118 (68.64%), Query Frame = 0


Query: 1   MVVSVPLFGCHNILGYAFTSDKQIANHIASMWPFICLSILNDSFLGILSGVARGTGWQRL 60
           ++VS+ L    ++ G+ F+SDK+   ++A M P + +SI+ DS  G+LSGVA G GWQ +
Sbjct: 347 LMVSMSLLAGRHVFGHVFSSDKKTIEYVAKMAPLVSISIILDSLQGVLSGVASGCGWQHI 406

Query: 61  GAYVNFGSYYIVGTPMAVVLCFVAHLRIKGLWIGLVSGAALQGFLFALITASTNWHKQ 119
           GAY+NFG++Y+ G P+A  L F  HL+  GLWIG+++GA LQ  L AL+T  TNW  Q
Sbjct: 407 GAYINFGAFYLWGIPIAASLAFWVHLKGVGLWIGILAGAVLQTLLLALVTGCTNWKTQ 464

The following BLAST results are available for this feature:

BLAST of Lag0039580 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6592362.1	1.7e-49	75.94	Protein DETOXIFICATION 14, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022925516.1	1.7e-49	75.94	protein DETOXIFICATION 8-like isoform X3 [Cucurbita moschata]	[more]
XP_022925514.1	1.7e-49	75.94	protein DETOXIFICATION 8-like isoform X1 [Cucurbita moschata]	[more]
XP_022925515.1	1.7e-49	75.94	protein DETOXIFICATION 3-like isoform X2 [Cucurbita moschata]	[more]
XP_022973955.1	3.7e-49	75.19	protein DETOXIFICATION 8-like isoform X3 [Cucurbita maxima]	[more]

BLAST of Lag0039580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HQ05	4.7e-31	50.43	Protein DETOXIFICATION 8 OS=Arabidopsis thaliana OX=3702 GN=DTX8 PE=3 SV=1	[more]
Q9SIA5	1.7e-28	46.96	Protein DETOXIFICATION 1 OS=Arabidopsis thaliana OX=3702 GN=DTX1 PE=2 SV=1	[more]
Q9C9M8	2.2e-28	42.52	Protein DETOXIFICATION 9 OS=Arabidopsis thaliana OX=3702 GN=DTX9 PE=2 SV=1	[more]
Q9SIA4	2.9e-28	47.83	Protein DETOXIFICATION 3 OS=Arabidopsis thaliana OX=3702 GN=DTX3 PE=3 SV=1	[more]
Q8VYL8	3.7e-28	47.46	Protein DETOXIFICATION 10 OS=Arabidopsis thaliana OX=3702 GN=DTX10 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002528	Multi antimicrobial extrusion protein	PFAM	PF01554	MatE	coord: 8..78 e-value: 2.9E-9 score: 36.8
None	No IPR available	PANTHER	PTHR11206:SF345	PROTEIN DETOXIFICATION	coord: 1..123
None	No IPR available	PANTHER	PTHR11206	MULTIDRUG RESISTANCE PROTEIN	coord: 1..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0039580.1	Lag0039580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990961	xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015297	antiporter activity
molecular_function	GO:0042910	xenobiotic transmembrane transporter activity