Lag0038545 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0038545 vs. NCBI nr
Match: XP_023521223.1 (neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo] >XP_023522087.1 neurofilament medium polypeptide-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-154
Identity = 325/463 (70.19%), Postives = 366/463 (79.05%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIVITING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GTRIEI WGQPVQKMEITRW YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKL +GILGVGI+EVKEQE G+A FESQD +AE IPH+  DLET ETG++T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEQEAGKATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEF GENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA------------HNGKI 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEV E TSQ TA            HNG +
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQKEKEGNAKEHNGDM 306

Query: 305 TTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T I K+PDVKTQSQASID+E +   R     +R T+AE  K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQSQASIDQEQENFKR-----NRHTRAELGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAYEHEEKGVDQQGKKIETEDD--KAREECKSPQE-THEDSSKEAKQEK 424
           PED E PYLQSPA+EHEEK VDQ+GK  +T+D+  +AREEC SPQ+ TH+DSSKEAKQEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEKEVDQKGKSSQTKDETKEAREECNSPQKVTHQDSSKEAKQEK 426

Query: 425 KKDPKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 453
               KQFKMR PII+AGSAL+ASLVIVVF+LVKSK+REKNMRG
Sbjct: 427 ----KQFKMRIPIIVAGSALVASLVIVVFSLVKSKKREKNMRG 446

BLAST of Lag0038545 vs. NCBI nr
Match: KAG6607636.1 (hypothetical protein SDJN03_00978, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037240.1 hypothetical protein SDJN02_00863, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 537.7 bits (1384), Expect = 9.5e-149
Identity = 313/463 (67.60%), Postives = 359/463 (77.54%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIV+TING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVVTINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GTRIEI WGQPVQKMEITRW YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  DLET ETG++T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGE  GENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSESE+L
Sbjct: 187 -------GNGELGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESERL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA------------HNGKI 304
           E  ATK TE E T+EN+NG+IKVPKV   EGIEV E TSQ TA            HNG +
Sbjct: 247 ETGATKATEYEGTKENINGQIKVPKVTITEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T I K+PDVKTQ QASID+E +   R     +R T+ E  K ETY+N  +L      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQEQENFKR-----NRHTRVELGKPETYRNTWKL------KE 366

Query: 365 PEDTESPYLQSPAYEHEEKGVDQQGKKIETEDD--KAREECKSPQE-THEDSSKEAKQEK 424
           PED E PYLQSPA+EHEEK VDQ+GK  +T+D+  +AREEC SPQ+ TH+DSSKEAKQEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEKEVDQKGKSFQTKDETKEAREECNSPQKVTHQDSSKEAKQEK 426

Query: 425 KKDPKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 453
               KQFKMR PII+AGSAL+A+LVI VF+LVKSK+REKNMRG
Sbjct: 427 ----KQFKMRIPIIVAGSALVATLVIAVFSLVKSKKREKNMRG 446

BLAST of Lag0038545 vs. NCBI nr
Match: XP_022143634.1 (uncharacterized protein LOC111013495 [Momordica charantia])

HSP 1 Score: 511.9 bits (1317), Expect = 5.6e-141
Identity = 311/478 (65.06%), Postives = 361/478 (75.52%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVIT 60
           MG++FELKISP+ +    S FELQLA+ T +PF+ SNET SMFILTAKLTGF+R+DI IT
Sbjct: 34  MGLDFELKISPSSS---FSNFELQLARGTPSPFW-SNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGNGTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESI 120
           ING GTRIEIGWGQPVQ+MEITRWMYKKETETR+FKKIFRIPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVE 180
           LKI+MPKLVDGILGVGIEEVKEQ+VGE A ESQD +AEEIP  T   E  ETGR+T+ VE
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVT---EGFETGRETKFVE 213

Query: 181 DDNHENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSE 240
                    DAANG F G NA PE   K EY+AET QEE EKLK DE E IDGVSN YSE
Sbjct: 214 ---------DAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEHIDGVSNHYSE 273

Query: 241 SEQLENVATKTTEIEATREN---VNGEIKVPKVRSMEGIEVTEETSQGT----------- 300
           SE+ E + T+  E     +N   V+ EI +PKVRS++GIE+ E TS+GT           
Sbjct: 274 SEKSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPN 333

Query: 301 --AHNGKITTKINKQPDVKTQSQASIDREAQVLDR-EFSRPDRQTQAE------QRKQET 360
             AHNG I T  NKQPD+K QSQA++DR+ +V D+ E S+P+ + QAE      Q  +ET
Sbjct: 334 SEAHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKET 393

Query: 361 YKNVRELEELETNKEPEDTESPYLQSPAYEHEEKGVDQQGKKIETEDD--KAREECKSPQ 420
           Y++  E +E ETN EPE   SPYLQSP +EHEEKGV+QQGK  +T+D+  +AREE  SPQ
Sbjct: 394 YRSAWESKEFETNNEPEYIASPYLQSPEHEHEEKGVNQQGKSFQTQDEIKEAREESGSPQ 453

Query: 421 -ETHEDSSKEAKQEKKKDPKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 453
            E HE S+KEAKQEK +D KQFKMRTPIIIAGSALLASL IVVFN V+SK+REKNMRG
Sbjct: 454 REKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Lag0038545 vs. NCBI nr
Match: XP_022926215.1 (neurofilament medium polypeptide-like [Cucurbita moschata])

HSP 1 Score: 465.7 bits (1197), Expect = 4.6e-127
Identity = 269/405 (66.42%), Postives = 306/405 (75.56%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIVITING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GTRIEI WGQPVQKMEITRW YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  DLET ETG++T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEF GENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSE EQL
Sbjct: 187 -------GNGEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSECEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA-------------HNGK 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEV E TSQ TA             HNG 
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEHNGD 306

Query: 305 ITTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNK 364
           I T I K+ DVKTQ QASID+EAQ L++E  + +R T+AE  K ETY+N REL      K
Sbjct: 307 IATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTREL------K 366

Query: 365 EPEDTESPYLQSPAYEHEEKGVDQQGK--------KIETEDDKAR 389
           EPED E PYLQSPA+EHEEK +  + K        K++T  D+ R
Sbjct: 367 EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVR 397

BLAST of Lag0038545 vs. NCBI nr
Match: XP_022981685.1 (uncharacterized protein LOC111480753 [Cucurbita maxima])

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-123
Identity = 262/379 (69.13%), Postives = 292/379 (77.04%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIVITING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GT+IEI WGQPVQKMEITR  YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKLV+GILGVGIE VKEQE G+A FESQD +AE IPH+  DLET ETG+KT+LVED + 
Sbjct: 127 MPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEF GENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA------------HNGKI 304
           E  ATK TE E T+EN+NG+I+VPKV SMEGIEV E TSQ TA            HNG +
Sbjct: 247 ETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T I K+PDVKTQ QASID+E     +E  + +R T+A   K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQE-----QENFKQNRHTRAGLGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAYEHEEK 372
           PED E PYLQSPA+EHEEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEK 366

BLAST of Lag0038545 vs. ExPASy TrEMBL
Match: A0A6J1CPW6 (uncharacterized protein LOC111013495 OS=Momordica charantia OX=3673 GN=LOC111013495 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 2.7e-141
Identity = 311/478 (65.06%), Postives = 361/478 (75.52%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVIT 60
           MG++FELKISP+ +    S FELQLA+ T +PF+ SNET SMFILTAKLTGF+R+DI IT
Sbjct: 34  MGLDFELKISPSSS---FSNFELQLARGTPSPFW-SNETNSMFILTAKLTGFSREDIGIT 93

Query: 61  INGNGTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESI 120
           ING GTRIEIGWGQPVQ+MEITRWMYKKETETR+FKKIFRIPDGVVLDEIKATYNDEESI
Sbjct: 94  INGTGTRIEIGWGQPVQRMEITRWMYKKETETRRFKKIFRIPDGVVLDEIKATYNDEESI 153

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVE 180
           LKI+MPKLVDGILGVGIEEVKEQ+VGE A ESQD +AEEIP  T   E  ETGR+T+ VE
Sbjct: 154 LKIVMPKLVDGILGVGIEEVKEQDVGEDALESQDSEAEEIPEVT---EGFETGRETKFVE 213

Query: 181 DDNHENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSE 240
                    DAANG F G NA PE   K EY+AET QEE EKLK DE E IDGVSN YSE
Sbjct: 214 ---------DAANGVFEGANATPEPSPKREYLAETTQEETEKLKGDEMEHIDGVSNHYSE 273

Query: 241 SEQLENVATKTTEIEATREN---VNGEIKVPKVRSMEGIEVTEETSQGT----------- 300
           SE+ E + T+  E     +N   V+ EI +PKVRS++GIE+ E TS+GT           
Sbjct: 274 SEKSEKLTTEIAEAPKRWDNGGIVDSEIIIPKVRSVKGIELDEGTSKGTANHQKEKGEPN 333

Query: 301 --AHNGKITTKINKQPDVKTQSQASIDREAQVLDR-EFSRPDRQTQAE------QRKQET 360
             AHNG I T  NKQPD+K QSQA++DR+ +V D+ E S+P+ + QAE      Q  +ET
Sbjct: 334 SEAHNGSIATMTNKQPDIKIQSQAAVDRDTKVPDKPELSQPNGKIQAEHKIVEQQGNKET 393

Query: 361 YKNVRELEELETNKEPEDTESPYLQSPAYEHEEKGVDQQGKKIETEDD--KAREECKSPQ 420
           Y++  E +E ETN EPE   SPYLQSP +EHEEKGV+QQGK  +T+D+  +AREE  SPQ
Sbjct: 394 YRSAWESKEFETNNEPEYIASPYLQSPEHEHEEKGVNQQGKSFQTQDEIKEAREESGSPQ 453

Query: 421 -ETHEDSSKEAKQEKKKDPKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 453
            E HE S+KEAKQEK +D KQFKMRTPIIIAGSALLASL IVVFN V+SK+REKNMRG
Sbjct: 454 REKHEQSTKEAKQEKIQDSKQFKMRTPIIIAGSALLASLAIVVFNFVRSKKREKNMRG 495

BLAST of Lag0038545 vs. ExPASy TrEMBL
Match: A0A6J1EKH3 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111433395 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 2.2e-127
Identity = 269/405 (66.42%), Postives = 306/405 (75.56%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIVITING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GTRIEI WGQPVQKMEITRW YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTRIEISWGQPVQKMEITRWTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKL +GILGVGI+EVKE+E GEA FESQD +AE IPH+  DLET ETG++T+L+ED + 
Sbjct: 127 MPKLEEGILGVGIQEVKEKEAGEATFESQDSEAEAIPHTPTDLETPETGKETKLIEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEF GENA+ EAFSK+EY AET  EEIEKLK +E ED DG SNDYSE EQL
Sbjct: 187 -------GNGEFGGENAKREAFSKMEYAAETTPEEIEKLKGNEMEDTDGGSNDYSECEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA-------------HNGK 304
           E  ATK TE E T+EN+NG+IKVPKV SMEGIEV E TSQ TA             HNG 
Sbjct: 247 ETGATKATEYEGTKENMNGQIKVPKVTSMEGIEVAEGTSQETANHQEEKGEGKAEEHNGD 306

Query: 305 ITTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNK 364
           I T I K+ DVKTQ QASID+EAQ L++E  + +R T+AE  K ETY+N REL      K
Sbjct: 307 IATMIKKKLDVKTQPQASIDQEAQTLEQENFKRNRHTRAELGKLETYRNTREL------K 366

Query: 365 EPEDTESPYLQSPAYEHEEKGVDQQGK--------KIETEDDKAR 389
           EPED E PYLQSPA+EHEEK +  + K        K++T  D+ R
Sbjct: 367 EPEDIEIPYLQSPAHEHEEKEISDEKKTTQSGHTTKVKTGRDEVR 397

BLAST of Lag0038545 vs. ExPASy TrEMBL
Match: A0A6J1J0B1 (uncharacterized protein LOC111480753 OS=Cucurbita maxima OX=3661 GN=LOC111480753 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 5.1e-124
Identity = 262/379 (69.13%), Postives = 292/379 (77.04%), Query Frame = 0

Query: 5   FELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVITINGN 64
           FELKISPTR DDPSSTFEL L K T+T  F S+ET SMF+LTAKL GFARDDIVITING+
Sbjct: 7   FELKISPTREDDPSSTFELHLLKTTSTHSFFSDETNSMFLLTAKLPGFARDDIVITINGS 66

Query: 65  GTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKIL 124
           GT+IEI WGQPVQKMEITR  YKKETETR+FKKIFRIPDGV+LDEIKATYNDE+SILKIL
Sbjct: 67  GTQIEISWGQPVQKMEITRLTYKKETETRRFKKIFRIPDGVILDEIKATYNDEKSILKIL 126

Query: 125 MPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETGRKTRLVEDDNH 184
           MPKLV+GILGVGIE VKEQE G+A FESQD +AE IPH+  DLET ETG+KT+LVED + 
Sbjct: 127 MPKLVEGILGVGIEGVKEQEAGDATFESQDSEAEAIPHAPTDLETPETGKKTKLVEDTH- 186

Query: 185 ENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQL 244
                   NGEF GENA+ EAFSKIEY AET  EEIEKLK +E ED DG SNDYSESEQL
Sbjct: 187 -------GNGEFGGENAKQEAFSKIEYAAETTPEEIEKLKGNEMEDTDGGSNDYSESEQL 246

Query: 245 ENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGTA------------HNGKI 304
           E  ATK TE E T+EN+NG+I+VPKV SMEGIEV E TSQ TA            HNG +
Sbjct: 247 ETGATKATEYEGTKENINGQIQVPKVTSMEGIEVAEGTSQETANHQKEKEGNAEEHNGDM 306

Query: 305 TTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNKE 364
            T I K+PDVKTQ QASID+E     +E  + +R T+A   K ETY+N REL      KE
Sbjct: 307 ATMIKKKPDVKTQPQASIDQE-----QENFKQNRHTRAGLGKPETYRNTREL------KE 366

Query: 365 PEDTESPYLQSPAYEHEEK 372
           PED E PYLQSPA+EHEEK
Sbjct: 367 PEDIEIPYLQSPAHEHEEK 366

BLAST of Lag0038545 vs. ExPASy TrEMBL
Match: A0A5A7UWU5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold518G00820 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.2e-43
Identity = 177/471 (37.58%), Postives = 221/471 (46.92%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVIT 60
           MG +FELKI PT  DD SSTFELQLA+ +AT        P    L   + G       +T
Sbjct: 49  MGSDFELKIKPTIDDDTSSTFELQLAEGSAT---LMKPIPCFATLVRNVIGTLVRKSDVT 108

Query: 61  INGNGTRIEIGWGQPVQKMEITRWMYKKETETRKFKKIFRIPDGVVLDEIKATYNDEESI 120
           +             P Q++    W     +E  K+  +  +     ++ IK    D + +
Sbjct: 109 L-------------PPQQLAYGVWF----SEVLKYLTMLSMSTHFGVEPIK----DSDQV 168

Query: 121 LKILMPKLVDGILGVGIEEVKEQEVGEAAFESQDYDAE-----EIPHSTADLETLETGRK 180
               +P+                        S D D       ++      L  +   RK
Sbjct: 169 WPSEVPR------------------------SNDRDRSCLRLWKLAERPTWLTVIMKRRK 228

Query: 181 TRLVEDDNHENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVS 240
             L+ +             +   +NAE E   KIEYVAE   +EIEKLK  E ++     
Sbjct: 229 PMLLME-------------QLKKKNAETERLHKIEYVAEIQPDEIEKLKGSEMDE----- 288

Query: 241 NDYSESEQLENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEETSQGT--------- 300
                                       +I VPK+RS+EGIEV E TS+GT         
Sbjct: 289 ----------------------------DIDVPKIRSVEGIEVAEVTSKGTASHKKETQT 348

Query: 301 ---AHNGKITTKINKQPDVKTQSQASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRE 360
              A NG   T INK+P VKTQSQASIDREA                           RE
Sbjct: 349 NAEARNGDKATMINKKPYVKTQSQASIDREA---------------------------RE 394

Query: 361 LEELETNKEPEDTESPYLQSPAYEHEEKGVDQQGKKIET--EDDKAREECKSPQETHEDS 420
           L+ELET+KEPED  SPYLQ PA +H+EKG D++G+  +T  E   AREECKS   THEDS
Sbjct: 409 LKELETDKEPEDIGSPYLQRPANQHDEKGADRKGRSFQTKYEVKDAREECKS---THEDS 394

Query: 421 SKEAKQEKKKDPKQFKMRTPIIIAGSALLASLVIVVFNLVKSKRREKNMRG 453
           SKEAKQEKK+D KQ KMRT IIIAGSALLASLV V FNLVKSK  EKN++G
Sbjct: 469 SKEAKQEKKQDSKQLKMRTSIIIAGSALLASLVTVAFNLVKSK-TEKNVKG 394

BLAST of Lag0038545 vs. ExPASy TrEMBL
Match: A0A5D3CSD6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1081G00020 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.1e-41
Identity = 135/268 (50.37%), Postives = 155/268 (57.84%), Query Frame = 0

Query: 199 ENAEPEAFSKIEYVAETPQEEIEKLKSDETEDIDGVSNDYSESEQLENVATKTTEIEATR 258
           +NAE E   KIEYVAE   +EIEKLK  E ++                            
Sbjct: 37  KNAETERLHKIEYVAEIQPDEIEKLKGSEMDE---------------------------- 96

Query: 259 ENVNGEIKVPKVRSMEGIEVTEETSQGT------------AHNGKITTKINKQPDVKTQS 318
                +I VPK+RS+EGIEV E TS+GT            A NG   T INK+P VKTQS
Sbjct: 97  -----DIDVPKIRSVEGIEVAEVTSKGTASHKKETQTNAEARNGDKATMINKKPYVKTQS 156

Query: 319 QASIDREAQVLDREFSRPDRQTQAEQRKQETYKNVRELEELETNKEPEDTESPYLQSPAY 378
           QASIDREA                           REL+ELET+KEPED  SPYLQ PA 
Sbjct: 157 QASIDREA---------------------------RELKELETDKEPEDIGSPYLQRPAN 216

Query: 379 EHEEKGVDQQGKKIET--EDDKAREECKSPQETHEDSSKEAKQEKKKDPKQFKMRTPIII 438
           +H+EKG D++G+  +T  E   AREECKS   THEDSSKEAKQEKK+D KQ KMRT III
Sbjct: 217 QHDEKGADRKGRSFQTKYEVKDAREECKS---THEDSSKEAKQEKKQDSKQLKMRTSIII 240

Query: 439 AGSALLASLVIVVFNLVKSKRREKNMRG 453
           AGSALLASLV V FNLVKSK  EKN++G
Sbjct: 277 AGSALLASLVTVAFNLVKSK-TEKNVKG 240

BLAST of Lag0038545 vs. TAIR 10
Match: AT1G76770.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-24
Identity = 84/228 (36.84%), Postives = 122/228 (53.51%), Query Frame = 0

Query: 1   MGMEFELKISPTRADDPSSTFELQLAKATATPFFCSNETPSMFILTAKLTGFARDDIVIT 60
           M +E  LKI+ T+ DD SS+ + ++A+ +      S ET S+F L   L GF +D I I 
Sbjct: 1   MELELGLKITRTK-DDVSSSTDFRVARDSFGHRSLSRETNSVFFLILHLKGFKKDGIDIE 60

Query: 61  INGNGTRIEIGWGQPVQKMEITRWM-YKKETETRKFKKIFRIPDGVVLDEIKATYNDEES 120
           IN  G  I+I   + V++M + +W+ +KKETE ++FKK+FRIPD V+LD+IKA +N+E+ 
Sbjct: 61  INKEGNLIKISGRKQVEEMVLVKWVEWKKETEIKEFKKVFRIPDIVILDKIKARFNEEDG 120

Query: 121 ILKILMPKLVDGILGVGIEEVKEQE-----VGEAAFESQDYDAEEIPHSTADLETLETGR 180
            L + MPK V GI G+ IEE  E+E     + E   E +    EEI   T   E  E   
Sbjct: 121 TLTVTMPKKVKGITGLKIEEEDEEEEMKEPIVEEKTEEKTEPEEEIKEETKPEEENEE-- 180

Query: 181 KTRLVEDDNHENEEDDAANGEFVGENAEPEAFSKIEYVAETPQEEIEK 223
                E+   E EE      E V E        K E + + P+++  K
Sbjct: 181 ----AEEPQREEEE------EVVEEGTRDHEGKKEEEIEDKPRKKRRK 215

BLAST of Lag0038545 vs. TAIR 10
Match: AT1G76780.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 3.9e-07
Identity = 95/351 (27.07%), Postives = 153/351 (43.59%), Query Frame = 0

Query: 79  MEITRWM-YKKETETRKFKKIFRIPDGVVLDEIKATYNDEESILKILMPKLVDGILGVGI 138
           M + RWM ++KE E   FKK+FRIPD V LD+IKA ++D+++ L I MPK V GI G  I
Sbjct: 1   MVMIRWMAWRKEVEFSVFKKVFRIPDTVDLDKIKARFDDDDATLTITMPKRVKGISGFKI 60

Query: 139 EEVKEQEVGEAAFESQDYDAEEIPHSTADLETLETG-------RKTRLVEDDNHENEEDD 198
           EE +E+E      E +  D  E  H     E  E G        K+  +++   E +   
Sbjct: 61  EEEEEEE----EEEEERVDVSEAEHK----EETEKGELKDDYLEKSHQIDERIEEEKGLA 120

Query: 199 AANGEFVGEN-AEPEAFSKIEYVAETPQEEIEKLK------SDETEDIDGVSNDYSESEQ 258
            +N E V  +  +P      E   +T  EE E  K      SD+++D    S  + E E+
Sbjct: 121 DSNKESVDSSLRKPPDIEGRECHEQTRHEEQENNKQLVQAESDDSDDFG--SRAFEEIEE 180

Query: 259 LENVATKTTEIEATRENVNGEIKVPKVRSMEGIEVTEE-----------TSQGTAHNGKI 318
            E+     T      E    +     +R ++GIE  E              + + H  K 
Sbjct: 181 QESDVLDRTSTSGAMEKEMTDDVGDGLRKVQGIEEPERHNEESKISEMVDGETSGHEKKK 240

Query: 319 TTKINKQ-PDVKTQ--------SQASIDREAQVLDREFS---RPDRQTQAEQRKQETYKN 378
             K++K+  DVK +         + +IDR   V D E +   + D + ++++ + +    
Sbjct: 241 VVKMDKKNRDVKEEVDGAMGEGFRPNIDRTQVVGDDEIAETEKNDEEFESDKLEADEVDK 300

Query: 379 VRELEELETNKEPEDTESPYLQSPAYEH--EEKGVDQQGKKIETEDDKARE 390
           + E    +  +  ED     LQ    +H  E+KG  ++    E  ++K  E
Sbjct: 301 INEGGNTKVRRHSEDRNLIKLQEKEEQHSKEQKGHSKEENMKELVEEKTPE 341