Lag0038483 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038483
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	rRNA N-glycosidase
Location	chr2: 18464823 .. 18465746 (-)
RNA-Seq Expression	Lag0038483
Synteny	Lag0038483

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTGACTCAAGAACTCAACACTTCACACAAGCTTTCCCCTTCGACTGCGGTGCTCATGCTGCCAACCGTGTCCGCGGTCGTGCCGTCGCCAATGCCAATGCCAGCTTCTCTCTGGTCCATGCCACTGGCGCAACATACAAACAATTCATAAGCGATCTGCGTAGGGCACTCACAGTGAACTCTCAAATAGTGTACGGCATACCCGTATTGCCAGACCCGACAGGCTTAGCACGCTTCATATTAGTCAACCTCACCAATTACAACGGCGAATCCATCACCGTGGCAATCAAGGTAGTCGACGCTTACGTCGTCGCATACCAAGCAGGAAACAAGGCCTACTTTCTAAACGACGTTTACGACGAACTAAGACAAGTACTATTTAAAGGTACCGAAAGCGTACGACTTCCCTTCAAAGGGAATTACGACGACCTCGAGAGGCGTGCAGGATCGAGAGAGAAAATTGACCTGGGATTCTCTGAATTAGGCAGTGCCATTGGCAATCTATTCCACTATAATAATAAAGATGTTACTTCTGCTGCCAGAGGGTTTATTGTGATCATTCAAACGCTTGCAGAAGCGGCGAGATTTAAGTACATTGAGCAAAGAGTTTCTGAGAATGTTGAGTCGAAGTTCAAGCCAGATCCGGCATTCTTAAGCTTGGAGAACCGTTGGAAGGATCTGTCTAAACAGATACTGTTGGCGCAAAAGCATGGCGGGAAATTTGAGCATCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTCAAAGACGTTAGTTCGCCAGTTGTTAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGACCAATCCATCTGATGATGATGGAGCAGTGTTTTGAGCAATGGGAAGATGCTCGAGGTGACTTTGGAGTAGATTTCTGA

mRNA sequence

ATGGAGTGCATTGACTCAAGAACTCAACACTTCACACAAGCTTTCCCCTTCGACTGCGGTGCTCATGCTGCCAACCGTGTCCGCGGTCGTGCCGTCGCCAATGCCAATGCCAGCTTCTCTCTGGTCCATGCCACTGGCGCAACATACAAACAATTCATAAGCGATCTGCGTAGGGCACTCACAGTGAACTCTCAAATAGTGTACGGCATACCCGTATTGCCAGACCCGACAGGCTTAGCACGCTTCATATTAGTCAACCTCACCAATTACAACGGCGAATCCATCACCGTGGCAATCAAGGTAGTCGACGCTTACGTCGTCGCATACCAAGCAGGAAACAAGGCCTACTTTCTAAACGACGTTTACGACGAACTAAGACAAGTACTATTTAAAGGTACCGAAAGCGTACGACTTCCCTTCAAAGGGAATTACGACGACCTCGAGAGGCGTGCAGGATCGAGAGAGAAAATTGACCTGGGATTCTCTGAATTAGGCAGTGCCATTGGCAATCTATTCCACTATAATAATAAAGATGTTACTTCTGCTGCCAGAGGGTTTATTGTGATCATTCAAACGCTTGCAGAAGCGGCGAGATTTAAGTACATTGAGCAAAGAGTTTCTGAGAATGTTGAGTCGAAGTTCAAGCCAGATCCGGCATTCTTAAGCTTGGAGAACCGTTGGAAGGATCTGTCTAAACAGATACTGTTGGCGCAAAAGCATGGCGGGAAATTTGAGCATCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTCAAAGACGTTAGTTCGCCAGTTGTTAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGACCAATCCATCTGATGATGATGGAGCAGTGTTTTGAGCAATGGGAAGATGCTCGAGGTGACTTTGGAGTAGATTTCTGA

Coding sequence (CDS)

ATGGAGTGCATTGACTCAAGAACTCAACACTTCACACAAGCTTTCCCCTTCGACTGCGGTGCTCATGCTGCCAACCGTGTCCGCGGTCGTGCCGTCGCCAATGCCAATGCCAGCTTCTCTCTGGTCCATGCCACTGGCGCAACATACAAACAATTCATAAGCGATCTGCGTAGGGCACTCACAGTGAACTCTCAAATAGTGTACGGCATACCCGTATTGCCAGACCCGACAGGCTTAGCACGCTTCATATTAGTCAACCTCACCAATTACAACGGCGAATCCATCACCGTGGCAATCAAGGTAGTCGACGCTTACGTCGTCGCATACCAAGCAGGAAACAAGGCCTACTTTCTAAACGACGTTTACGACGAACTAAGACAAGTACTATTTAAAGGTACCGAAAGCGTACGACTTCCCTTCAAAGGGAATTACGACGACCTCGAGAGGCGTGCAGGATCGAGAGAGAAAATTGACCTGGGATTCTCTGAATTAGGCAGTGCCATTGGCAATCTATTCCACTATAATAATAAAGATGTTACTTCTGCTGCCAGAGGGTTTATTGTGATCATTCAAACGCTTGCAGAAGCGGCGAGATTTAAGTACATTGAGCAAAGAGTTTCTGAGAATGTTGAGTCGAAGTTCAAGCCAGATCCGGCATTCTTAAGCTTGGAGAACCGTTGGAAGGATCTGTCTAAACAGATACTGTTGGCGCAAAAGCATGGCGGGAAATTTGAGCATCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTCAAAGACGTTAGTTCGCCAGTTGTTAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGACCAATCCATCTGATGATGATGGAGCAGTGTTTTGAGCAATGGGAAGATGCTCGAGGTGACTTTGGAGTAGATTTCTGA

Protein sequence

MECIDSRTQHFTQAFPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPDPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDLERRAGSREKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQWEDARGDFGVDF

Homology

[Close]

BLAST of Lag0038483 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 374.0 bits (959), Expect = 1.2e-99
Identity = 204/310 (65.81%), Postives = 228/310 (73.55%), Query Frame = 0


Query: 1   MECIDSRTQHFTQAFPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRAL 60
           MECI S        FP +CGAH A R   R  ANAN SFSL+ AT  TYKQFISDLR+AL
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRA-PRPSANANLSFSLLGATAQTYKQFISDLRKAL 60

Query: 61  TVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFLN 120
           TVNS  VY IPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL 
Sbjct: 61  TVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLK 120

Query: 121 DVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNNK 180
           DV  E   VLFKG++   L +KGNYDDLER AG  SRE IDLGFSEL S+IGN+FHYN  
Sbjct: 121 DVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAG 180

Query: 181 DVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILLA 240
              S  + FIV+IQ L+EA+RFKYIE R  ENVESKFKPDP +LSLENRW +LS+Q+  A
Sbjct: 181 --YSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQWE 300
           QKHGGKFEHPV VRSVSNKP+LV DV+SPVVKG+ALL         +  +M E  F +WE
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSKLMEE--FIKWE 300

Query: 301 DARGDFGVDF 308
            ARG FG+DF
Sbjct: 301 AARGHFGIDF 305

[Close]

BLAST of Lag0038483 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-99
Identity = 208/311 (66.88%), Postives = 232/311 (74.60%), Query Frame = 0


Query: 1   MECIDSRTQHFTQA-FPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MECI S TQ   QA FP +CGAHAA R   R  ANAN SFSL+ AT  TYKQFI+DLR+A
Sbjct: 1   MECIGS-TQDLIQAPFPLNCGAHAAIRA-PRPSANANLSFSLLGATAQTYKQFINDLRKA 60

Query: 61  LTVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LTVNS  VY IPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL
Sbjct: 61  LTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            DV  E   VLFKG++   L +KGNYDDLER AG  SRE I+LGFSEL S+IGN+FHY  
Sbjct: 121 KDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHY-- 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
           K   S  R FIV+IQ L+EA+RFKYIE R  E VESKFKPDP +LSLENRW +LS+Q+  
Sbjct: 181 KAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQKHGGKFEHPV VRSVSNKP+LV DV+SPVVKGIALL +       +  +M E  F  W
Sbjct: 241 AQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEE--FIHW 300

Query: 301 EDARGDFGVDF 308
           E ARG FG+DF
Sbjct: 301 EAARGHFGIDF 305

[Close]

BLAST of Lag0038483 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.5 bits (950), Expect = 1.4e-98
Identity = 207/311 (66.56%), Postives = 232/311 (74.60%), Query Frame = 0


Query: 1   MECIDSRTQHFTQA-FPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MECI S TQ   QA FP +CGAHAA R   R  ANAN SFSL+ AT  TYKQFI+DLR+A
Sbjct: 1   MECIGS-TQDLIQAPFPLNCGAHAAIRA-PRPSANANLSFSLLGATAQTYKQFINDLRKA 60

Query: 61  LTVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LTVNS  VY IPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL
Sbjct: 61  LTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            DV  E   VLFKG++   L +KGNYDDLER AG  SRE I+LGFSEL S+IGN+FHYN 
Sbjct: 121 KDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYNA 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
               S  R FIV+IQ L+EA+RFKYIE R  E VESKFKPDP +LSLENRW +LS+Q+  
Sbjct: 181 G--YSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQKHGGKFEHPV VRSVSNKP+LV DV+SPVVKGIALL         +  +M E  F +W
Sbjct: 241 AQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVSKLMEE--FIKW 300

Query: 301 EDARGDFGVDF 308
           E ARG FG++F
Sbjct: 301 EAARGAFGINF 305

[Close]

BLAST of Lag0038483 vs. NCBI nr
Match: KAG7037234.1 (hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 366.3 bits (939), Expect = 2.6e-97
Identity = 206/311 (66.24%), Postives = 229/311 (73.63%), Query Frame = 0


Query: 1   MECIDSRTQHFTQA-FPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MECI S TQ   QA FP +CGAHAA RV  R  ANAN SFSL+ AT  TYKQFI DLR+A
Sbjct: 1   MECIGS-TQDLIQAPFPLNCGAHAAIRV-PRPSANANVSFSLLGATAETYKQFIKDLRKA 60

Query: 61  LTVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LTVNS  VYGIPVLP    GLARFILVNLTNYNGESITVAI  VD Y+VAYQAGNKAYFL
Sbjct: 61  LTVNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            DV  E   VLFKG++   L +KGNYDDLER AG  SRE + LGFSEL S+IGN+FHYN 
Sbjct: 121 KDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNA 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
               S  R FIV+IQ L+EA+RFKYIE+  S+NV S FKPDP +LSLENRW +LS+Q+  
Sbjct: 181 G--YSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQK 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQKHGGKFEH V VRSV NK I V DV+SPVVKGIALL         +  +M E  F  W
Sbjct: 241 AQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANGVSKLMEE--FIHW 300

Query: 301 EDARGDFGVDF 308
           E ARGDFG+DF
Sbjct: 301 EAARGDFGIDF 305

[Close]

BLAST of Lag0038483 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365.5 bits (937), Expect = 4.4e-97
Identity = 206/311 (66.24%), Postives = 229/311 (73.63%), Query Frame = 0


Query: 1   MECIDSRTQHFTQA-FPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MECI S TQ   QA FP +CGAHAA R   R  ANAN SFSL+ AT  TYKQFI+DLR A
Sbjct: 1   MECIGS-TQDLIQAPFPLNCGAHAAIRA-PRPSANANLSFSLLGATAQTYKQFINDLRNA 60

Query: 61  LTVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LTV S  VYGIPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL
Sbjct: 61  LTVQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            DV  E   VLFKG++   L +KGNYDDLER AG  SRE I+LGFSEL S+IGN+FHYN 
Sbjct: 121 KDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNA 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
               S  R FIV+IQ L+EA+RFKYIE R  E VESKFKPDP +LSLENRW +LS+Q+  
Sbjct: 181 G--YSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQKHGGKFE PV VRSVSNKPI V DV+SPVVKGIALL     + G   +  + + F  W
Sbjct: 241 AQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALL--LCNKPGVNGVSKLMEQFIHW 300

Query: 301 EDARGDFGVDF 308
           E ARG FG+DF
Sbjct: 301 EAARGAFGIDF 305

[Close]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.4e-76
Identity = 157/251 (62.55%), Postives = 184/251 (73.31%), Query Frame = 0


Query: 27  VRGRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPD-PTGLARFILV 86
           V      + + +FSL+ ATGATYK FI +LR  LTV +  VY IPVL +   GLARF LV
Sbjct: 14  VGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLV 73

Query: 87  NLTNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYD 146
            LTNYNGES+TVA+ VV+ YVVAY+AGN AYFL D   E   VLF G   VRLP+ GNYD
Sbjct: 74  TLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYD 133

Query: 147 DLERRAG--SREKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIE 206
            LE  AG  SRE I+LGFSE+ SAIGN+F +N    TS  R FIVIIQT++EAARFKYIE
Sbjct: 134 GLETAAGRISRENIELGFSEISSAIGNMFRHN--PGTSVPRAFIVIIQTVSEAARFKYIE 193

Query: 207 QRVSENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDV 266
           QRVSENV +KFKPDPAFLSL+N W  LS+QI +AQ  GG+F  PV +R+VSN P  V +V
Sbjct: 194 QRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNV 253

Query: 267 SSPVVKGIALL 275
           +SPVVKGIALL
Sbjct: 254 NSPVVKGIALL 262

[Close]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 2.7e-49
Identity = 119/252 (47.22%), Postives = 156/252 (61.90%), Query Frame = 0


Query: 29  GRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPDP-TGLARFILVNL 88
           G   A  + +F L  AT  TY +FI D R  L   S  VY IP+L    +   RFIL+NL
Sbjct: 17  GVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNL 76

Query: 89  TNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDL 148
           T+Y  E+I+VAI V + YVVAY+  + +YF  +   E   +LFKGT  + LP+ GNY++L
Sbjct: 77  TSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENL 136

Query: 149 ERRAGS-REKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQRV 208
           +  A   RE IDLG   L SAI  LF+YN +   SA    +V+IQT AEAARFKYIE+ V
Sbjct: 137 QTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSA---LLVLIQTTAEAARFKYIERHV 196

Query: 209 SENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSSP 268
           ++ V + FKP+ A +SLEN+W  LSKQI LAQ  GGKF +PV +   + +   V +V S 
Sbjct: 197 AKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSD 256

Query: 269 VVKG-IALLRNT 278
           VVKG I LL N+
Sbjct: 257 VVKGNIKLLLNS 264

[Close]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-48
Identity = 118/252 (46.83%), Postives = 156/252 (61.90%), Query Frame = 0


Query: 29  GRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPDP-TGLARFILVNL 88
           G   A  + +F L  AT  TY +FI D R  L   S  VY IP+L    +   RFIL++L
Sbjct: 17  GVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDL 76

Query: 89  TNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDL 148
           T+Y  E+I+VAI V + YVVAY+  + +YF  +   E   +LFKGT  + LP+ GNY++L
Sbjct: 77  TSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENL 136

Query: 149 ERRAGS-REKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQRV 208
           +  A   RE IDLG   L SAI  LF+YN +   SA    +V+IQT AEAARFKYIE+ V
Sbjct: 137 QTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSA---LLVLIQTTAEAARFKYIERHV 196

Query: 209 SENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSSP 268
           ++ V + FKP+ A +SLEN+W  LSKQI LAQ  GGKF +PV +   + +   V +V S 
Sbjct: 197 AKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSD 256

Query: 269 VVKG-IALLRNT 278
           VVKG I LL N+
Sbjct: 257 VVKGNIKLLLNS 264

[Close]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot
Match: Q5F0I3 (Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 6.6e-48
Identity = 114/258 (44.19%), Postives = 157/258 (60.85%), Query Frame = 0


Query: 29  GRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPD--PTGLARFILVN 88
           G  +A  + +FSL  A G TY  FI+ LR+ L++ +Q V  I VL     +   RF+ +N
Sbjct: 17  GFHIAECDINFSLAGADGQTYNTFIAKLRQELSIGTQKVANITVLKHHVSSNTQRFLSIN 76

Query: 89  LTNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDEL--RQVLFKGTESVRLPFKGNY 148
           L NYNG+ IT+ I V D YVV +  G  +Y   +  D    + +LF G+    L + G Y
Sbjct: 77  LFNYNGDKITLGIDVFDVYVVGFLTGTNSYIFKEAPDLAYNKSLLFPGSVRENLSYTGGY 136

Query: 149 DDLERRAGSREKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQ 208
           DDLERR   RE I LG   L +AI NLF    +D TS  R FIVIIQ ++EAARFK IE 
Sbjct: 137 DDLERRGAGREDIPLGLLPLDTAITNLF---RRDSTSFRRSFIVIIQMVSEAARFKIIEA 196

Query: 209 RVSENV--ESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKD 268
           ++++N+  E+ FKPD A +SLEN W  LSKQI  AQ  GG F + V + + S KP+++++
Sbjct: 197 KIAKNLYGENTFKPDQAIISLENNWGALSKQIQKAQDRGGVFPNLVTLTTSSGKPLIIRN 256

Query: 269 VSSPVVK-GIALLRNTSK 280
            S P+V+ GIALL+  S+
Sbjct: 257 DSDPLVQNGIALLKYMSE 271

[Close]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 6.6e-48
Identity = 111/244 (45.49%), Postives = 152/244 (62.30%), Query Frame = 0


Query: 36  NASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPDPT-GLARFILVNLTNYNGES 95
           N  F L  AT ++YK FI +LR AL  + + VY IPVL        RFIL++L NY+G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGK-VYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 96  ITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDLERRAGS- 155
           IT AI V++ Y+VAY  G  +YF   V  +  ++LFKGT+   LP+ GNY++L+  A   
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 156 REKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESK 215
           RE I+LG   L SAI  LFHYN     +AA   +V+IQT +EAARF+YIE +++ NV +K
Sbjct: 121 RENIELGLPALDSAITTLFHYN---AEAAASALLVLIQTTSEAARFRYIELQIANNVGTK 180

Query: 216 FKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSSPVV-KGIA 275
           FKP    +SLEN W  LSKQI +A+   G+FE PV +       + + +V+S VV + I 
Sbjct: 181 FKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQ 240

Query: 276 LLRN 277
           LL N
Sbjct: 241 LLLN 240

[Close]

BLAST of Lag0038483 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 5.9e-100
Identity = 204/310 (65.81%), Postives = 228/310 (73.55%), Query Frame = 0


Query: 1   MECIDSRTQHFTQAFPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRAL 60
           MECI S        FP +CGAH A R   R  ANAN SFSL+ AT  TYKQFISDLR+AL
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRA-PRPSANANLSFSLLGATAQTYKQFISDLRKAL 60

Query: 61  TVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFLN 120
           TVNS  VY IPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL 
Sbjct: 61  TVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLK 120

Query: 121 DVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNNK 180
           DV  E   VLFKG++   L +KGNYDDLER AG  SRE IDLGFSEL S+IGN+FHYN  
Sbjct: 121 DVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAG 180

Query: 181 DVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILLA 240
              S  + FIV+IQ L+EA+RFKYIE R  ENVESKFKPDP +LSLENRW +LS+Q+  A
Sbjct: 181 --YSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQWE 300
           QKHGGKFEHPV VRSVSNKP+LV DV+SPVVKG+ALL         +  +M E  F +WE
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSKLMEE--FIKWE 300

Query: 301 DARGDFGVDF 308
            ARG FG+DF
Sbjct: 301 AARGHFGIDF 305

[Close]

BLAST of Lag0038483 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.0e-99
Identity = 208/311 (66.88%), Postives = 232/311 (74.60%), Query Frame = 0


Query: 1   MECIDSRTQHFTQA-FPFDCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MECI S TQ   QA FP +CGAHAA R   R  ANAN SFSL+ AT  TYKQFI+DLR+A
Sbjct: 1   MECIGS-TQDLIQAPFPLNCGAHAAIRA-PRPSANANLSFSLLGATAQTYKQFINDLRKA 60

Query: 61  LTVNSQIVYGIPVLP-DPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LTVNS  VY IPVLP    GLARFILVNLTNY GESITVAI  VD Y+VAYQAGNKAYFL
Sbjct: 61  LTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            DV  E   VLFKG++   L +KGNYDDLER AG  SRE I+LGFSEL S+IGN+FHY  
Sbjct: 121 KDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHY-- 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
           K   S  R FIV+IQ L+EA+RFKYIE R  E VESKFKPDP +LSLENRW +LS+Q+  
Sbjct: 181 KAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQKHGGKFEHPV VRSVSNKP+LV DV+SPVVKGIALL +       +  +M E  F  W
Sbjct: 241 AQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEE--FIHW 300

Query: 301 EDARGDFGVDF 308
           E ARG FG+DF
Sbjct: 301 EAARGHFGIDF 305

[Close]

BLAST of Lag0038483 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 6.6e-91
Identity = 184/311 (59.16%), Postives = 226/311 (72.67%), Query Frame = 0


Query: 1   MECIDSRTQHFTQAFPF-DCGAHAANRVRGRAVANANASFSLVHATGATYKQFISDLRRA 60
           MEC+ S ++ F Q  PF D G H  +        N+N SFSL+ ATG TY+QFI +LR A
Sbjct: 1   MECVRS-SEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNA 60

Query: 61  LTVNSQIVYGIPVL-PDPTGLARFILVNLTNYNGESITVAIKVVDAYVVAYQAGNKAYFL 120
           LT+NS+IVY IPVL       ARFILV+LTNY  E+ITVAI VV+ Y+VAYQAGNKAYFL
Sbjct: 61  LTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELRQVLFKGTESVRLPFKGNYDDLERRAG--SREKIDLGFSELGSAIGNLFHYNN 180
            D   E R VLFKG +   LP+KGNYD LE  AG  SREKIDLGFSELGSAIGN++HYN 
Sbjct: 121 KDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNA 180

Query: 181 KDVTSAARGFIVIIQTLAEAARFKYIEQRVSENVESKFKPDPAFLSLENRWKDLSKQILL 240
              TS  R FIV+IQT++EAARFKYIE +VS+NVE+KFKPDP FLSLENRW DLS+Q+ +
Sbjct: 181 G--TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQI 240

Query: 241 AQKHGGKFEHPVPVRSVSNKPILVKDVSSPVVKGIALLRNTSKEDGPIHLMMMEQCFEQW 300
           AQ   G+F  P+ VRSV+NKPILV +V S VV+G+ALL  +        + +ME+  ++W
Sbjct: 241 AQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEW 300

Query: 301 EDARGDFGVDF 308
           E+ RG+FG+DF
Sbjct: 301 ENGRGEFGIDF 308

[Close]

BLAST of Lag0038483 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 7.3e-82
Identity = 167/249 (67.07%), Postives = 196/249 (78.71%), Query Frame = 0


Query: 29  GRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVLPD-PTGLARFILVNL 88
           G   A A+ SFS++ AT  TY+QFI +LR  LT+ S +VYGIPVL     G ARFILV+L
Sbjct: 15  GALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHL 74

Query: 89  TNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDL 148
           TNYN ESITVAI VV+ YVVAY+AGN AYFLND   E   VLFKG + VRLP+KGNYD L
Sbjct: 75  TNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGL 134

Query: 149 ERRAG--SREKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQR 208
           E  AG  SREKIDLGFSE+ S+IGN+FH+N    TS AR FIVIIQ++ EAARFKYIEQR
Sbjct: 135 ETAAGKISREKIDLGFSEISSSIGNMFHHNVG--TSIARAFIVIIQSVLEAARFKYIEQR 194

Query: 209 VSENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSS 268
           VSENV++KFKPDPAFLSLENRW DLS+QI +AQK  G+F   + +R+VSNKPI+V +VSS
Sbjct: 195 VSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSS 254

Query: 269 PVVKGIALL 275
           P+VKGIALL
Sbjct: 255 PIVKGIALL 261

[Close]

BLAST of Lag0038483 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 4.0e-80
Identity = 166/248 (66.94%), Postives = 195/248 (78.63%), Query Frame = 0


Query: 29  GRAVANANASFSLVHATGATYKQFISDLRRALTVNSQIVYGIPVL-PDPTGLARFILVNL 88
           G   A A+ SFS++ +T  TYKQFI ++R ALTV SQIVY IPVL    TG ARF+LV+L
Sbjct: 15  GARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHL 74

Query: 89  TNYNGESITVAIKVVDAYVVAYQAGNKAYFLNDVYDELRQVLFKGTESVRLPFKGNYDDL 148
           TNYN ESI VAI VV+ YVVAY+AGN AYFLND   E  +VLF+G   V+L +KGNYD L
Sbjct: 75  TNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGL 134

Query: 149 ERRAG-SREKIDLGFSELGSAIGNLFHYNNKDVTSAARGFIVIIQTLAEAARFKYIEQRV 208
           E  A  SREKIDLGFSE+ S+IGN+FH+N    TS  R FIVIIQ+++EAARFKYIEQRV
Sbjct: 135 ETAAKISREKIDLGFSEISSSIGNMFHHNAG--TSVPRAFIVIIQSVSEAARFKYIEQRV 194

Query: 209 SENVESKFKPDPAFLSLENRWKDLSKQILLAQKHGGKFEHPVPVRSVSNKPILVKDVSSP 268
           SENV++ FKPDPAFLSL+NRW DLS+QI +AQK GG+F   V +R+VSNKPILV DVSSP
Sbjct: 195 SENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSP 254

Query: 269 VVKGIALL 275
           VVKGIALL
Sbjct: 255 VVKGIALL 260

The following BLAST results are available for this feature:

BLAST of Lag0038483 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022981562.1	1.2e-99	65.81	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
XP_022926255.1	2.1e-99	66.88	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
KAG7037232.1	1.4e-98	66.56	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG7037234.1	2.6e-97	66.24	hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023523582.1	4.4e-97	66.24	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0038483 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	4.4e-76	62.55	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	2.7e-49	47.22	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.0e-48	46.83	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
Q5F0I3	6.6e-48	44.19	Ribosome-inactivating protein gynostemmin OS=Gynostemma pentaphyllum OX=182084 P...	[more]
P84531	6.6e-48	45.49	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]

BLAST of Lag0038483 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IUB2	5.9e-100	65.81	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1EKL0	1.0e-99	66.88	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1E093	6.6e-91	59.16	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DWK4	7.3e-82	67.07	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	4.0e-80	66.94	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]

BLAST of Lag0038483 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 47..60 score: 40.6 coord: 218..235 score: 42.98 coord: 154..168 score: 49.47 coord: 186..206 score: 58.65 coord: 94..109 score: 47.86
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 198..280 e-value: 1.0E-22 score: 81.8
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 35..197 e-value: 3.8E-45 score: 155.7
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 47..235 e-value: 5.9E-37 score: 127.2
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 39..281
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 39..281
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 190..206
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 38..277

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038483.1	Lag0038483.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity