Lag0038431 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038431
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	CCHC-type domain-containing protein
Location	chr2: 17314348 .. 17315819 (+)
RNA-Seq Expression	Lag0038431
Synteny	Lag0038431

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGATGAGGGCAACATGGAAGTTTGCTCAGAGGGGTCCAACGGTGACCAAGAGATCACGCCGAAGAAGACAGTGAACGATGCTATTGAAGTGAGAAGCCTGCAAGAAAGAGAAGATGTAACAGCTAATCAAATGGGAACAAGAGACGTGGAAGAAGATCAAGACAGGGAGCAAGAGACAATTCTCAGCAAACTCGAAAAGCTGAAGATTGAGGAAAAGAAGAGAATAGTGGCGGTCGAAGATGTTGATATAGAGGAGGACACAGATAAAGAATTATTGTATGTGGTAGCATGCAAAGTATTATCTGAGAAGAGTGTCAACGAGGAGTATTTCATCTCTATGATGCCAAGAATATGGGGCATAGAAGGCTACGACAAGATAAAAAGAGCAGGAAGGAATATCTATCAATGCAACTTCAAATTCTTGAGAGACAAAACCAAAATAATTAAGGGGGACCATGGAGTTTTGATAATGCACTTCTGATCTTTGAAGATCTAAGAGGAAACTGTAGTTTAAACTCTTTAAATTTTAGATATGTCTCCTTTTGGGTCCATTTTAAAAACTTACCAAGAGTTTGTTTTTGTAGGAAATATGCTGAAGCTTTGGGGAATTCAATAGGAAGATTTGAAGAGGTCGAAGCAGATGAGGAAGGACAAATGGGAGGAGAGTCTTTGAGAGTGGAAATACAAGTGGATGTGAGGGAACCTCTGAAAAGGGGAACTCATGTTAAGACGGGCACAAAAGGAGAAACAAAATGGATTCCTATTACCTACGAAAAACTTCCAGACTTTTGCTATTTCTGTGGGAAGCTGGGTCATGTTTTTCAAGAATGCAGGGAGGAAGGTGCAGAGGGGGAAATCGAGCTAAGCTATGGAGTGGGGATGAGAGAAACTCAAACTAGTAAAGGAGTGTATAAACACCCAAAAGCCCAGTTCAGATTTCAGAGTTTCAGAGGTAGAGGAAGGGGAAGGTTCTACAGCACAGGGGAAGAGTTCCAAAGCAAATCTTGGAGGGTCAAAAATAGAAGAGAACTAGAGATTGAAAATCAAGAGATGCATCAACAAAATATGGAAGCTACTAATCAAAAAGAAAAAACCTCCGAGATCTCTGAAGGAAGGAAAGTTTCTAAAACGACGATGAATCAAGGTGGCGAAGCAATGGAGTCAGTTAGGTCGGCGATGAACTTTCCGGCAGTCGTGGACGAGAGGAGGAGAGGGGAAAAAGAGACGTTAGAAAAAGACAAAAAGATTCAGAACCCGAAGTCATTTTCTGCAGAAAGAGAAAATGATGTAGACAGGAAAAATGATGAGAATAGTAAACAAAAAGACAAGGGTCAATGTCAGAAGAAAAAGATGATTTTCACACAAAAAGACAAGTCCAAGACAGCAATCACACCGTTAAAACTCAACCTGGCAATGGGCCCAATCTTAATCAGCATTCCAAAAGCCCAACGGGGAAAGAAAAAGTAA

mRNA sequence

ATGGACGATGAGGGCAACATGGAAGTTTGCTCAGAGGGGTCCAACGGTGACCAAGAGATCACGCCGAAGAAGACAGTGAACGATGCTATTGAAGTGAGAAGCCTGCAAGAAAGAGAAGATGTAACAGCTAATCAAATGGGAACAAGAGACGTGGAAGAAGATCAAGACAGGGAGCAAGAGACAATTCTCAGCAAACTCGAAAAGCTGAAGATTGAGGAAAAGAAGAGAATAGTGGCGGTCGAAGATGTTGATATAGAGGAGGACACAGATAAAGAATTATTGAAATATGCTGAAGCTTTGGGGAATTCAATAGGAAGATTTGAAGAGGTCGAAGCAGATGAGGAAGGACAAATGGGAGGAGAGTCTTTGAGAGTGGAAATACAAGTGGATGTGAGGGAACCTCTGAAAAGGGGAACTCATGTTAAGACGGGCACAAAAGGAGAAACAAAATGGATTCCTATTACCTACGAAAAACTTCCAGACTTTTGCTATTTCTGTGGGAAGCTGGGTCATGTTTTTCAAGAATGCAGGGAGGAAGGTGCAGAGGGGGAAATCGAGCTAAGCTATGGAGTGGGGATGAGAGAAACTCAAACTAGTAAAGGAGTGTATAAACACCCAAAAGCCCAGTTCAGATTTCAGAGTTTCAGAGGTAGAGGAAGGGGAAGGTTCTACAGCACAGGGGAAGAGTTCCAAAGCAAATCTTGGAGGGTCAAAAATAGAAGAGAACTAGAGATTGAAAATCAAGAGATGCATCAACAAAATATGGAAGCTACTAATCAAAAAGAAAAAACCTCCGAGATCTCTGAAGGAAGGAAAGTTTCTAAAACGACGATGAATCAAGGTGGCGAAGCAATGGAGTCAGTTAGGTCGGCGATGAACTTTCCGGCAGTCGTGGACGAGAGGAGGAGAGGGGAAAAAGAGACGTTAGAAAAAGACAAAAAGATTCAGAACCCGAAGTCATTTTCTGCAGAAAGAGAAAATGATGTAGACAGGAAAAATGATGAGAATAGTAAACAAAAAGACAAGGGTCAATGTCAGAAGAAAAAGATGATTTTCACACAAAAAGACAAGTCCAAGACAGCAATCACACCGTTAAAACTCAACCTGGCAATGGGCCCAATCTTAATCAGCATTCCAAAAGCCCAACGGGGAAAGAAAAAGTAA

Coding sequence (CDS)

ATGGACGATGAGGGCAACATGGAAGTTTGCTCAGAGGGGTCCAACGGTGACCAAGAGATCACGCCGAAGAAGACAGTGAACGATGCTATTGAAGTGAGAAGCCTGCAAGAAAGAGAAGATGTAACAGCTAATCAAATGGGAACAAGAGACGTGGAAGAAGATCAAGACAGGGAGCAAGAGACAATTCTCAGCAAACTCGAAAAGCTGAAGATTGAGGAAAAGAAGAGAATAGTGGCGGTCGAAGATGTTGATATAGAGGAGGACACAGATAAAGAATTATTGAAATATGCTGAAGCTTTGGGGAATTCAATAGGAAGATTTGAAGAGGTCGAAGCAGATGAGGAAGGACAAATGGGAGGAGAGTCTTTGAGAGTGGAAATACAAGTGGATGTGAGGGAACCTCTGAAAAGGGGAACTCATGTTAAGACGGGCACAAAAGGAGAAACAAAATGGATTCCTATTACCTACGAAAAACTTCCAGACTTTTGCTATTTCTGTGGGAAGCTGGGTCATGTTTTTCAAGAATGCAGGGAGGAAGGTGCAGAGGGGGAAATCGAGCTAAGCTATGGAGTGGGGATGAGAGAAACTCAAACTAGTAAAGGAGTGTATAAACACCCAAAAGCCCAGTTCAGATTTCAGAGTTTCAGAGGTAGAGGAAGGGGAAGGTTCTACAGCACAGGGGAAGAGTTCCAAAGCAAATCTTGGAGGGTCAAAAATAGAAGAGAACTAGAGATTGAAAATCAAGAGATGCATCAACAAAATATGGAAGCTACTAATCAAAAAGAAAAAACCTCCGAGATCTCTGAAGGAAGGAAAGTTTCTAAAACGACGATGAATCAAGGTGGCGAAGCAATGGAGTCAGTTAGGTCGGCGATGAACTTTCCGGCAGTCGTGGACGAGAGGAGGAGAGGGGAAAAAGAGACGTTAGAAAAAGACAAAAAGATTCAGAACCCGAAGTCATTTTCTGCAGAAAGAGAAAATGATGTAGACAGGAAAAATGATGAGAATAGTAAACAAAAAGACAAGGGTCAATGTCAGAAGAAAAAGATGATTTTCACACAAAAAGACAAGTCCAAGACAGCAATCACACCGTTAAAACTCAACCTGGCAATGGGCCCAATCTTAATCAGCATTCCAAAAGCCCAACGGGGAAAGAAAAAGTAA

Protein sequence

MDDEGNMEVCSEGSNGDQEITPKKTVNDAIEVRSLQEREDVTANQMGTRDVEEDQDREQETILSKLEKLKIEEKKRIVAVEDVDIEEDTDKELLKYAEALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITYEKLPDFCYFCGKLGHVFQECREEGAEGEIELSYGVGMRETQTSKGVYKHPKAQFRFQSFRGRGRGRFYSTGEEFQSKSWRVKNRRELEIENQEMHQQNMEATNQKEKTSEISEGRKVSKTTMNQGGEAMESVRSAMNFPAVVDERRRGEKETLEKDKKIQNPKSFSAERENDVDRKNDENSKQKDKGQCQKKKMIFTQKDKSKTAITPLKLNLAMGPILISIPKAQRGKKK

Homology

[Close]

BLAST of Lag0038431 vs. NCBI nr
Match: PON92154.1 (Zinc knuckle CX2CX4HX4C [Trema orientale])

HSP 1 Score: 96.3 bits (238), Expect = 6.2e-16
Identity = 45/102 (44.12%), Postives = 63/102 (61.76%), Query Frame = 0


Query: 97  AEALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITY 156
           A+ LGN  GRF EVE D+ G   G  L V + VD+ +PL+RG  V+ G+ G T W+   Y
Sbjct: 14  AKLLGNQAGRFVEVEKDDNGSCLGRFLNVRVIVDINKPLRRGAKVRIGSGGHTIWVDFRY 73

Query: 157 EKLPDFCYFCGKLGHVFQECREEGAE----GEIELSYGVGMR 195
            +LPDFC++CGK+GH   +C EE  +    G+ E +YG  +R
Sbjct: 74  VRLPDFCFWCGKIGHTMADCFEEIPDKVRRGKQEPNYGAWLR 115

[Close]

BLAST of Lag0038431 vs. NCBI nr
Match: KAA0046998.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK04784.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 93.2 bits (230), Expect = 5.3e-15
Identity = 43/88 (48.86%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 134 PLKRGTHVKTGTKGETKWIPITYEKLPDFCYFCGKLGHVFQECREEGAEGEIELSYGVGM 193
           P   GT  K G   + KWIP++YEKLPDFCY CG++GHV QEC+E G +GE    +GV +
Sbjct: 37  PNVHGTIKKLGVMAKEKWIPVSYEKLPDFCYKCGRVGHVVQECKEVGDDGEEGQRFGVWL 96

Query: 194 RETQTSKGVYKHPKAQFRFQSFRGRGRG 222
           R++QTS+ + K  + + R Q    RGRG
Sbjct: 97  RKSQTSQSISKGRREEVRSQFTIDRGRG 124

[Close]

BLAST of Lag0038431 vs. NCBI nr
Match: CAD6257659.1 (unnamed protein product [Miscanthus lutarioriparius])

HSP 1 Score: 87.8 bits (216), Expect = 2.2e-13
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 97  AEALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITY 156
           A A+G+ +G F EV+AD      G  LR+++++D+R+PL RG     G     +W PITY
Sbjct: 223 ARAIGDDVGEFMEVDADGSDLAAGRDLRLKVRMDIRKPLCRGILADLGPNKGDRWCPITY 282

Query: 157 EKLPDFCYFCGKLGHVFQECREEGAEGE 185
           E  PDFCY CG +GHV + C ++  +GE
Sbjct: 283 EHFPDFCYICGLIGHVDRACLKKLGKGE 310

[Close]

BLAST of Lag0038431 vs. NCBI nr
Match: BAT10513.1 (Os10g0362600 [Oryza sativa Japonica Group])

HSP 1 Score: 87.4 bits (215), Expect = 2.9e-13
Identity = 37/89 (41.57%), Postives = 56/89 (62.92%), Query Frame = 0


Query: 99  ALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITYEK 158
           A+G  +G F  ++ +E+G   G+ LR++I++D+R+PL RG  +  G      W P+ YE 
Sbjct: 47  AIGREVGEFMTMDLEEDGSAVGQFLRIKIRIDIRKPLMRGVTLFVGADERPLWCPLVYEF 106

Query: 159 LPDFCYFCGKLGHVFQECREEGAEGEIEL 188
           LPDFCY CG +GH  + C ++ AEGE  L
Sbjct: 107 LPDFCYICGIVGHTEKLCEKKLAEGEAPL 135

[Close]

BLAST of Lag0038431 vs. NCBI nr
Match: KAE8782072.1 (hypothetical protein D1007_44590 [Hordeum vulgare])

HSP 1 Score: 86.7 bits (213), Expect = 4.9e-13
Identity = 39/97 (40.21%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 98  EALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITYE 157
           E +G  +G F +VE  E+G   GESLR+++++D+ +PL RGT + TG     +W P  YE
Sbjct: 112 EMIGEKVGAFVDVEVGEDGMAEGESLRIKVKLDITKPLMRGTVIHTGDDERVRWCPFRYE 171

Query: 158 KLPDFCYFCGKLGHVFQECREEGAEGEIELSYGVGMR 195
            L +FCY CG +GH  + C     +GE E  +G  +R
Sbjct: 172 YLREFCYICGIIGHEEKNCSVSLTKGE-EKQFGSWLR 207

[Close]

BLAST of Lag0038431 vs. ExPASy TrEMBL
Match: A0A2P5F2Z2 (Zinc knuckle CX2CX4HX4C OS=Trema orientale OX=63057 GN=TorRG33x02_119730 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-16
Identity = 45/102 (44.12%), Postives = 63/102 (61.76%), Query Frame = 0


Query: 97  AEALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITY 156
           A+ LGN  GRF EVE D+ G   G  L V + VD+ +PL+RG  V+ G+ G T W+   Y
Sbjct: 14  AKLLGNQAGRFVEVEKDDNGSCLGRFLNVRVIVDINKPLRRGAKVRIGSGGHTIWVDFRY 73

Query: 157 EKLPDFCYFCGKLGHVFQECREEGAE----GEIELSYGVGMR 195
            +LPDFC++CGK+GH   +C EE  +    G+ E +YG  +R
Sbjct: 74  VRLPDFCFWCGKIGHTMADCFEEIPDKVRRGKQEPNYGAWLR 115

[Close]

BLAST of Lag0038431 vs. ExPASy TrEMBL
Match: A0A5D3C0K2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001040 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.6e-15
Identity = 43/88 (48.86%), Postives = 58/88 (65.91%), Query Frame = 0


Query: 134 PLKRGTHVKTGTKGETKWIPITYEKLPDFCYFCGKLGHVFQECREEGAEGEIELSYGVGM 193
           P   GT  K G   + KWIP++YEKLPDFCY CG++GHV QEC+E G +GE    +GV +
Sbjct: 37  PNVHGTIKKLGVMAKEKWIPVSYEKLPDFCYKCGRVGHVVQECKEVGDDGEEGQRFGVWL 96

Query: 194 RETQTSKGVYKHPKAQFRFQSFRGRGRG 222
           R++QTS+ + K  + + R Q    RGRG
Sbjct: 97  RKSQTSQSISKGRREEVRSQFTIDRGRG 124

[Close]

BLAST of Lag0038431 vs. ExPASy TrEMBL
Match: A0A0A0LNB5 (CCHC-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G427330 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.8e-14
Identity = 44/82 (53.66%), Postives = 54/82 (65.85%), Query Frame = 0


Query: 95  KYAEALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPI 154
           KY  AL NSI  FEEVE  E  +  GE L+V  ++DV++ L   T++K G   + K IP+
Sbjct: 32  KYVVALTNSIDSFEEVEESELRRTYGELLKVREKIDVQQSLNHETNIKLGAMAKEKCIPV 91

Query: 155 TYEKLPDFCYFCGKLGHVFQEC 177
           TYEKL DFCY CG LGHV QEC
Sbjct: 92  TYEKLSDFCYNCGILGHVLQEC 113

[Close]

BLAST of Lag0038431 vs. ExPASy TrEMBL
Match: A0A0P0XTA0 (Os10g0362600 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0362600 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-13
Identity = 37/89 (41.57%), Postives = 56/89 (62.92%), Query Frame = 0


Query: 99  ALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITYEK 158
           A+G  +G F  ++ +E+G   G+ LR++I++D+R+PL RG  +  G      W P+ YE 
Sbjct: 47  AIGREVGEFMTMDLEEDGSAVGQFLRIKIRIDIRKPLMRGVTLFVGADERPLWCPLVYEF 106

Query: 159 LPDFCYFCGKLGHVFQECREEGAEGEIEL 188
           LPDFCY CG +GH  + C ++ AEGE  L
Sbjct: 107 LPDFCYICGIVGHTEKLCEKKLAEGEAPL 135

[Close]

BLAST of Lag0038431 vs. ExPASy TrEMBL
Match: Q2QQV8 (Retrotransposon protein, putative, unclassified OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os12g29700 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.0e-13
Identity = 49/141 (34.75%), Postives = 76/141 (53.90%), Query Frame = 0


Query: 99  ALGNSIGRFEEVEADEEGQMGGESLRVEIQVDVREPLKRGTHVKTGTKGETKWIPITYEK 158
           A+G  +G F  ++ +E+G   G+ LR++I++D+R+PL RG  +  G      W P+ YE 
Sbjct: 42  AIGREVGEFMTMDLEEDGSAVGQFLRIKIRIDIRKPLMRGVTLFVGADERPLWCPLVYEF 101

Query: 159 LPDFCYFCGKLGHVFQECREEGAEGEIELSYGVGMR---ETQTSKGVYKHPKAQFRFQSF 218
           LPDFCY CG +GH  + C ++ AEGE  L +   +R   E +  +G      +  RFQ  
Sbjct: 102 LPDFCYICGIVGHTEKLCEKKLAEGEAPL-FNKSLRFIPEQKRWEGGSSDKTSGSRFQVQ 161

Query: 219 RGRGRGRFYSTGEEFQSKSWR 237
           R  G     S+ +   + SWR
Sbjct: 162 RKSGA----SSCKGSDAPSWR 177

The following BLAST results are available for this feature:

BLAST of Lag0038431 vs. NCBI nr


Match Name	E-value	Identity	Description
PON92154.1	6.2e-16	44.12	Zinc knuckle CX2CX4HX4C [Trema orientale]	[more]
KAA0046998.1	5.3e-15	48.86	reverse transcriptase [Cucumis melo var. makuwa] >TYK04784.1 reverse transcripta...	[more]
CAD6257659.1	2.2e-13	43.18	unnamed protein product [Miscanthus lutarioriparius]	[more]
BAT10513.1	2.9e-13	41.57	Os10g0362600 [Oryza sativa Japonica Group]	[more]
KAE8782072.1	4.9e-13	40.21	hypothetical protein D1007_44590 [Hordeum vulgare]	[more]

BLAST of Lag0038431 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0038431 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2P5F2Z2	3.0e-16	44.12	Zinc knuckle CX2CX4HX4C OS=Trema orientale OX=63057 GN=TorRG33x02_119730 PE=4 SV...	[more]
A0A5D3C0K2	2.6e-15	48.86	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68...	[more]
A0A0A0LNB5	2.8e-14	53.66	CCHC-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G427330 P...	[more]
A0A0P0XTA0	1.4e-13	41.57	Os10g0362600 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0362600 PE...	[more]
Q2QQV8	7.0e-13	34.75	Retrotransposon protein, putative, unclassified OS=Oryza sativa subsp. japonica ...	[more]

BLAST of Lag0038431 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 240..263
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 296..355
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 271..286
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 250..360
None	No IPR available	PANTHER	PTHR31286:SF84	SUBFAMILY NOT NAMED	coord: 95..276
IPR025836	Zinc knuckle CX2CX4HX4C	PFAM	PF14392	zf-CCHC_4	coord: 129..177 e-value: 2.2E-14 score: 52.8
IPR040256	Uncharacterized protein At4g02000-like	PANTHER	PTHR31286	GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKE	coord: 95..276
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 163..178 score: 9.504374

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038431.1	Lag0038431.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding