Lag0037699 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037699
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description60S ribosomal protein L27
Locationchr2: 8470527 .. 8470934 (+)
RNA-Seq ExpressionLag0037699
SyntenyLag0037699
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

mRNA sequence

ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

Coding sequence (CDS)

ATGGTGAAATTTATCAAGCCGAACAAGGCAGTTATTGTTCTGCAAGGCCGATTCGCCGGCCGAAAGGCTGTGATCGTCCGCAACTTCGATGACGGAACCCGCGATCGCCCCTACGGCCATTGTTTGGTTGCAGGGATCAAAAAGTACCCGAGCAAGGTCATTCGCAAGGATTCAACGAAGAAGACGGCGAAGAAATCGAGAGTGAAGGCCTTCATCAAACTTGTGAACTACCAGCACCTCATGCCGACAAGGTACACGCTCGACGTCGATCTCAAGGATGTGGTTAATGTGGATTGTTTGCAGTCGAAGGGATCAAAGGTCGCAGCCGCAAAGGAAATCAAGAAGAGGTTGGAGGACAGGTTTAAGACTGGGAAGAACAGGTGGTTTTTTACCAAGCTTAGGTTCTGA

Protein sequence

MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTKKTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDRFKTGKNRWFFTKLRF
Homology
BLAST of Lag0037699 vs. NCBI nr
Match: XP_022981139.1 (60S ribosomal protein L27-like [Cucurbita maxima])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 132/135 (97.78%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVN DCLQSKGSKVAAAKEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNADCLQSKGSKVAAAKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. NCBI nr
Match: XP_023523697.1 (60S ribosomal protein L27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 6.0e-67
Identity = 131/135 (97.04%), Postives = 133/135 (98.52%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVN DCLQSKGSKV AAKEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNADCLQSKGSKVVAAKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. NCBI nr
Match: XP_022940098.1 (60S ribosomal protein L27-3-like [Cucurbita moschata])

HSP 1 Score: 262.7 bits (670), Expect = 1.8e-66
Identity = 131/135 (97.04%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQH+MPTRYTLDVDLKDVVNVDCLQSK SKVAA+KEIKKRLEDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHIMPTRYTLDVDLKDVVNVDCLQSKVSKVAASKEIKKRLEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. NCBI nr
Match: XP_022143316.1 (60S ribosomal protein L27-like [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 2.3e-66
Identity = 129/135 (95.56%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAV+VLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGI KYPS+VIRKDSTK
Sbjct: 1   MVKFIKPNKAVVVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGISKYPSRVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKG+KVAAAKEIKKR+E+R
Sbjct: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGTKVAAAKEIKKRMEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. NCBI nr
Match: XP_038899503.1 (60S ribosomal protein L27-3-like [Benincasa hispida])

HSP 1 Score: 261.5 bits (667), Expect = 3.9e-66
Identity = 130/135 (96.30%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKFIKPNKAVIVLQGRFAGRKAVIVRNFD+GTRDRPYG+CLVAG+KKYPSKVIRKDSTK
Sbjct: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDEGTRDRPYGYCLVAGMKKYPSKVIRKDSTK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAA+KEIKKR EDR
Sbjct: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAASKEIKKRFEDR 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. ExPASy Swiss-Prot
Match: P51419 (60S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 2.8e-59
Identity = 111/135 (82.22%), Postives = 123/135 (91.11%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+K NKAVI+LQGR+AG+KAVI+++FDDG RDRPYGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK FIKLVNYQHLMPTRYTLDVDLK+V  +D LQSK  KVAA KE K +LE+R
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. ExPASy Swiss-Prot
Match: Q05462 (60S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.8e-59
Identity = 112/135 (82.96%), Postives = 123/135 (91.11%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+KPNKAVI+LQGR+AG+KAVIV+ FDDGTR++PYGHCLVAGIKK+PSKVI+KDS K
Sbjct: 1   MVKFLKPNKAVILLQGRYAGKKAVIVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVKAF+KLVNYQHLMPTRYTLDVDLKD V  D LQSK  KV A KE KK LE+R
Sbjct: 61  KTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. ExPASy Swiss-Prot
Match: Q8LCL3 (60S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 3.4e-57
Identity = 108/135 (80.00%), Postives = 121/135 (89.63%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVKF+K NKAVI+LQGR+AG+KAVI+++FDDGT DR YGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK FIKLVNYQHLMPTRYTLDVDLK+V  +D L+SK  KV A KE K +LE+R
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. ExPASy Swiss-Prot
Match: Q9SKX8 (60S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 5.1e-53
Identity = 99/135 (73.33%), Postives = 117/135 (86.67%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           MVK +KP KAVI+LQGR+ G+KAVIV++FDDGT ++ YGHCLVAG+KKYPSKVIRKDS K
Sbjct: 1   MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDLKDVVNVDCLQSKGSKVAAAKEIKKRLEDR 120
           KTAKKSRVK F K++NYQH+MPTRYTLD+DLK+VV+ D + SK  KV A KE K + E+R
Sbjct: 61  KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

BLAST of Lag0037699 vs. ExPASy Swiss-Prot
Match: P41101 (60S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.0e-52
Identity = 105/139 (75.54%), Postives = 116/139 (83.45%), Query Frame = 0

Query: 1   MVKFIKPNKAVIVLQGRFAGRKAVIVRNFDDGTRDRPYGHCLVAGIKKYPSKVIRKDSTK 60
           M KF+KPNKAVI+LQG++AGRKAVIVR FD+GTRDRPYGHCLVAGI +YP KVIRKDS K
Sbjct: 1   MEKFLKPNKAVILLQGKYAGRKAVIVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKLVNYQHLMPTRYTLDVDL----KDVVNVDCLQSKGSKVAAAKEIKKR 120
           K  KKSRVKAFIKLVNY H+MPTRYTLD DL    KDVVN D LQ++  KV  A+  K R
Sbjct: 61  KAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKV-TARRTKAR 120

Query: 121 LEDRFKTGKNRWFFTKLRF 136
           L +RFKTGKNRWFFTKLRF
Sbjct: 121 LAERFKTGKNRWFFTKLRF 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022981139.12.1e-6797.7860S ribosomal protein L27-like [Cucurbita maxima][more]
XP_023523697.16.0e-6797.0460S ribosomal protein L27-like [Cucurbita pepo subsp. pepo][more]
XP_022940098.11.8e-6697.0460S ribosomal protein L27-3-like [Cucurbita moschata][more]
XP_022143316.12.3e-6695.5660S ribosomal protein L27-like [Momordica charantia][more]
XP_038899503.13.9e-6696.3060S ribosomal protein L27-3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P514192.8e-5982.2260S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2[more]
Q054622.8e-5982.9660S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1[more]
Q8LCL33.4e-5780.0060S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2[more]
Q9SKX85.1e-5373.3360S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1[more]
P411012.0e-5275.5460S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005824KOWSMARTSM00739kow_9coord: 4..31
e-value: 0.0031
score: 26.8
IPR038655Ribosomal protein L27e superfamilyGENE3D2.30.30.770coord: 1..135
e-value: 8.3E-61
score: 206.0
IPR001141Ribosomal protein L27ePFAMPF01777Ribosomal_L27ecoord: 52..135
e-value: 1.7E-31
score: 108.4
IPR001141Ribosomal protein L27ePANTHERPTHR1049760S RIBOSOMAL PROTEIN L27coord: 1..135
NoneNo IPR availablePANTHERPTHR10497:SF1660S RIBOSOMAL PROTEIN L27coord: 1..135
IPR018262Ribosomal protein L27e, conserved sitePROSITEPS01107RIBOSOMAL_L27Ecoord: 124..135
IPR041991Eukaryotic Ribosomal Protein L27, KOW domainCDDcd06090KOW_RPL27coord: 6..88
e-value: 7.31423E-43
score: 134.233
IPR008991Translation protein SH3-like domain superfamilySUPERFAMILY50104Translation proteins SH3-like domaincoord: 2..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037699.1Lag0037699.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0022625 cytosolic large ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003735 structural constituent of ribosome