Lag0037373 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0037373
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA-dependent metalloprotease WSS1-like isoform X2
Locationchr2: 5669038 .. 5671232 (-)
RNA-Seq ExpressionLag0037373
SyntenyLag0037373
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTGGATTTTTTTTTTGTTTGGCGCGGTTCCCTTCTCTCGTCTTCAAAACGCGTGGGTTTTTTATCGGCGCGGTGACTGGCCAACACACATTTCTCGGCGGCGACTTCATCTCTTCCCCTCACCGTCGGTCACTAAGCTCTTCATCTCCGACACGACGTGGGTTTTTTTATTTGTGGGTTTGTGTTCAAAGTGTAGATGTGTGTGGTTGTACTTCGTGGGTAGCAAATCCATGGTGGTCGTGGCTGTTCATGCTCAGATCTGAGGTCGTGGTGGTCGTTGGTAGCAAATCCGACATTAAAAGACAGTATATTTGAGCCCGATCAGTTTGATTTTAAGATTTGAGTGGTCGTGGCTGTTTATGTTGAATTTTCACTTCATTTCGTTTTTGTTGTAAGGAATGATGAAAAGAGTATCCATTTTCTTCCTTCATTCTGTTTATTGAAAATTTTGCAGCCTAAAAAATCCAGCACTTTTAGGGTTAAATGTGGGGCGTGGAATTCATGTGAAGCTGAGGCTTCGAAGGCCAAATAGGGATGAAGATTTCTTTCCCTTTAATCAAGTTTTGGATACAATGCTACACGAGCTTTGCCACAATCTCCATGGTCCTCACAATGCCAATTTCTACAAGCTTTGGGATGAACTTAGAAAGGTGTTTCATTTATGCTTGTTTTTTTTAATGGTTTTTTATTAGAATATGTGAAAAAAACACAGAAAATGATATACATGTTGATAATCAAAGGACTATCGTTCTCTTATTTGCTTGATTGTATCCATTCCATTTACATTTGTCCTTCTCGTTTAAGTTCCCCTGGCCGTATGCAAGGTTCTGTTTATTATAATCCATTGCATATCAATTGATGGACATTATAATGGTTAAAGCAAATTTAACTGATCAGAGATTAGAGAAGTGGCAATGGCTTTCTTGAAAAGAACTTTTCTTCAATGCAACTCCTAGTTTGTATTTTTATATTTTATTATAGGAAAATTTCATTCTGATTGGACATTGCCTCTTATCATGGATCAAATCTGATGGTTTGTATAAAATGTAGGAATGTGAGGAGTTGATGGCTAAGGGAATTAGTGGCACAGCACAGGGATTTGATCTCCCGGGGAGGCGTTTGGGTGGTAATACATGACAACCCCCTCTTTCTTCCCTCCGCAAATCTGCCCTAGCTGCTGCAGAAGGAAGAAAACGTTTGGGATCTCTACTTCCTTCTGGGCCTAATCGGTTTGGTGGTGATAGCAACATCATGGTTGCACTAAGTCCTGTACAAGCAGCTGCAATGGCTGCAGAAAGGAGGCTTCAGGATGATATTTGGTGTGCTTCATCTCAAGAATTGCCTGTGGATGAGGAATGTTGTTCTGATTTTCCATCAGAAGCGGTGCATCCCTCCCACGCCGGTACAAATGGATCTGAGGTCTCTCTCTTTTTCTCTTTTAATTTGTACTCTAGTTTGATTGTTGAAGTTATGTGGCGTTTAACATAAATGTACTCTGACTTTGACGTTGGGGTGAAAACTAAAATCAATAGTGTTTGGTTTTGGATGAAATGGTGTCTTGTCTAGCCTGGTTTCTCACTCTAGGTTCTATAATTTTGTTTTTGGCAATTATACTCTCAGCTAAATGAAAATAAAATTTTCATGGATTAGAAGGTTTCTTGAACTTTTGCAATTTAGTTTTTTTCATTTCTGTACTATGGCTGCGATTTGCGCCTGGATTGTAATAATAAGATGATTTTTCTATGAAGATTTTTGGTTTGCTTCAAAGCTTATTATAAAGTTTGATGCAAATACTCTAACTTAGTATATGATATACAATGTAAATTGAGCAGGCATTACAAGCAAGTTGGGAGCTAACACACTCAAGTCACGGCTACAGATTCCCTAGCATTCGCATGTGAATTTTGCAGCAACTGAGACTAGCAGTTCTTGCTAAAAGGACAATGGAAAAAAAAAAATGATTTACCTACTCTTCATTTTCAAAAAAATGGTACCTACCAGGATGAAGACTCATGGTAGGACATCATTTTTTGATATTTCATCAACCGCGGAATTATCATTTTTTCATTATTAGATTTTTTTTTCTTATCTCATTCTTATGGGTGGAATTTTCTGCTTTGTAGGAAGCGATTGATGAAAATGGATATTCTCAAAAAGGAGCTTGGAGAGGAACTTGGCCGGAAACAGCGTAG

mRNA sequence

ATGCGTGGATTTTTTTTTTGTTTGGCGCGGTTCCCTTCTCTCGTCTTCAAAACGCGTGGGTTTTTTATCGGCGCGGTGACTGGCCAACACACATTTCTCGGCGGCGACTTCATCTCTTCCCCTCACCGTCGGTCACTAAGCTCTTCATCTCCGACACGACGTGGGTTTTTTTATTTGTGGGTTTGTGTTCAAAGTGTAGATGTGTGTGGTTGTACTTCGTGGGTAGCAAATCCATGGTGGTCGTGGCTGTTCATGCTCAGATCTGAGGTCGTGGTGGTCGTTGGTAGCAAATCCGACATTAAAAGACACCTAAAAAATCCAGCACTTTTAGGGTTAAATGTGGGGCGTGGAATTCATGTGAAGCTGAGGCTTCGAAGGCCAAATAGGGATGAAGATTTCTTTCCCTTTAATCAAGTTTTGGATACAATGCTACACGAGCTTTGCCACAATCTCCATGGTCCTCACAATGCCAATTTCTACAAGCTTTGGGATGAACTTAGAAAGGAATGTGAGGAGTTGATGGCTAAGGGAATTAGTGGCACAGCACAGGGATTTGATCTCCCGGGGAGGCGTTTGGGTGCTGCTGCAGAAGGAAGAAAACGTTTGGGATCTCTACTTCCTTCTGGGCCTAATCGGTTTGGTGGTGATAGCAACATCATGGTTGCACTAAGTCCTGTACAAGCAGCTGCAATGGCTGCAGAAAGGAGGCTTCAGGATGATATTTGGTGTGCTTCATCTCAAGAATTGCCTGTGGATGAGGAATGTTGTTCTGATTTTCCATCAGAAGCGGTGCATCCCTCCCACGCCGGTACAAATGGATCTGAGGAAGCGATTGATGAAAATGGATATTCTCAAAAAGGAGCTTGGAGAGGAACTTGGCCGGAAACAGCGTAG

Coding sequence (CDS)

ATGCGTGGATTTTTTTTTTGTTTGGCGCGGTTCCCTTCTCTCGTCTTCAAAACGCGTGGGTTTTTTATCGGCGCGGTGACTGGCCAACACACATTTCTCGGCGGCGACTTCATCTCTTCCCCTCACCGTCGGTCACTAAGCTCTTCATCTCCGACACGACGTGGGTTTTTTTATTTGTGGGTTTGTGTTCAAAGTGTAGATGTGTGTGGTTGTACTTCGTGGGTAGCAAATCCATGGTGGTCGTGGCTGTTCATGCTCAGATCTGAGGTCGTGGTGGTCGTTGGTAGCAAATCCGACATTAAAAGACACCTAAAAAATCCAGCACTTTTAGGGTTAAATGTGGGGCGTGGAATTCATGTGAAGCTGAGGCTTCGAAGGCCAAATAGGGATGAAGATTTCTTTCCCTTTAATCAAGTTTTGGATACAATGCTACACGAGCTTTGCCACAATCTCCATGGTCCTCACAATGCCAATTTCTACAAGCTTTGGGATGAACTTAGAAAGGAATGTGAGGAGTTGATGGCTAAGGGAATTAGTGGCACAGCACAGGGATTTGATCTCCCGGGGAGGCGTTTGGGTGCTGCTGCAGAAGGAAGAAAACGTTTGGGATCTCTACTTCCTTCTGGGCCTAATCGGTTTGGTGGTGATAGCAACATCATGGTTGCACTAAGTCCTGTACAAGCAGCTGCAATGGCTGCAGAAAGGAGGCTTCAGGATGATATTTGGTGTGCTTCATCTCAAGAATTGCCTGTGGATGAGGAATGTTGTTCTGATTTTCCATCAGAAGCGGTGCATCCCTCCCACGCCGGTACAAATGGATCTGAGGAAGCGATTGATGAAAATGGATATTCTCAAAAAGGAGCTTGGAGAGGAACTTGGCCGGAAACAGCGTAG

Protein sequence

MRGFFFCLARFPSLVFKTRGFFIGAVTGQHTFLGGDFISSPHRRSLSSSSPTRRGFFYLWVCVQSVDVCGCTSWVANPWWSWLFMLRSEVVVVVGSKSDIKRHLKNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWDELRKECEELMAKGISGTAQGFDLPGRRLGAAAEGRKRLGSLLPSGPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSHAGTNGSEEAIDENGYSQKGAWRGTWPETA
Homology
BLAST of Lag0037373 vs. NCBI nr
Match: XP_023525391.1 (uncharacterized protein LOC111789010 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.8 bits (813), Expect = 1.0e-82
Identity = 157/185 (84.86%), Postives = 161/185 (87.03%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNANFYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNANFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPSE  H SH
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSETAHSSH 233

BLAST of Lag0037373 vs. NCBI nr
Match: XP_038898345.1 (uncharacterized protein LOC120086023 [Benincasa hispida] >XP_038898346.1 uncharacterized protein LOC120086023 [Benincasa hispida])

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-82
Identity = 156/185 (84.32%), Postives = 161/185 (87.03%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DF+PFNQVLDTMLHELCHNLHGPHNANFYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFYPFNQVLDTMLHELCHNLHGPHNANFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISGTAQGFDLPGRRLG                AAAEGR+ LGSLLPS
Sbjct: 114 ELRKECEELMAKGISGTAQGFDLPGRRLGGNVRQPPLSSLHKSSLAAAEGRRHLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDE+CC DFPSEA H S 
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEDCCPDFPSEAAHFSQ 233

BLAST of Lag0037373 vs. NCBI nr
Match: KAG6607757.1 (hypothetical protein SDJN03_01099, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 316.2 bits (809), Expect = 2.9e-82
Identity = 156/185 (84.32%), Postives = 162/185 (87.57%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNA+FYKLWD
Sbjct: 97  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNASFYKLWD 156

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 157 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 216

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPS+ VH SH
Sbjct: 217 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSDTVHSSH 276

BLAST of Lag0037373 vs. NCBI nr
Match: XP_022940664.1 (uncharacterized protein LOC111446189 isoform X1 [Cucurbita moschata])

HSP 1 Score: 316.2 bits (809), Expect = 2.9e-82
Identity = 156/185 (84.32%), Postives = 162/185 (87.57%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNA+FYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNASFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPS+ VH SH
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSDTVHSSH 233

BLAST of Lag0037373 vs. NCBI nr
Match: KAG7037334.1 (WSS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.8 bits (808), Expect = 3.8e-82
Identity = 156/185 (84.32%), Postives = 161/185 (87.03%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNA+FYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNASFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQSPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPSE  H SH
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSETAHSSH 233

BLAST of Lag0037373 vs. ExPASy Swiss-Prot
Match: P38838 (DNA-dependent metalloprotease WSS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=WSS1 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.4e-15
Identity = 59/165 (35.76%), Postives = 77/165 (46.67%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           ++  LLG+NV  G  + LRLR    +  F P   ++ TMLHEL HNL GPH+  FY   D
Sbjct: 75  RDQRLLGMNVNHGSKIMLRLRCSTDEFQFLPMECIMGTMLHELTHNLFGPHDKKFYNKLD 134

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLGAAAEGRK----------------------RL 224
           EL      +  +G+  T  G    G+RLG  A  R                       +L
Sbjct: 135 ELIGRQWVIEQRGLYDTFLG---NGQRLGGRANLRSNRYPMTGISTNTGIVRKRGKGVKL 194

Query: 225 GSLLPSGPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQ 248
           GSL P       G S+I    SP + AA AAERR +DD WC  ++
Sbjct: 195 GSLHPE------GISSIDRGNSPRELAAFAAERRYRDDRWCGETK 230

BLAST of Lag0037373 vs. ExPASy Swiss-Prot
Match: O94580 (DNA-dependent metalloprotease WSS1 homolog 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=wss2 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.0e-09
Identity = 32/87 (36.78%), Postives = 54/87 (62.07%), Query Frame = 0

Query: 96  SKSDIKRHLKNPA-LLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGP 155
           S+ D   H ++ +  LGLN  +G H++LRL R +R + F  +  V  T++HEL HN+HG 
Sbjct: 153 SEMDPAEHTRHDSKTLGLNHNQGAHIELRL-RTDRYDGFRDYKTVKSTLIHELTHNVHGE 212

Query: 156 HNANFYKLWDELRKECEELMAKGISGT 182
           H+++F++L+ +L KE +     G  G+
Sbjct: 213 HDSSFWELFRQLTKEADAADLLGKPGS 238

BLAST of Lag0037373 vs. ExPASy Swiss-Prot
Match: Q9P7B5 (DNA-dependent metalloprotease WSS1 homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC521.02 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-05
Identity = 28/75 (37.33%), Postives = 36/75 (48.00%), Query Frame = 0

Query: 106 NPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWDE 165
           N    G N  +G  ++L LR  +    + PF  V+D  LHELCH   GPH+  F+     
Sbjct: 80  NAKFWGRNWNKGECIELVLR--DASNRWLPFEFVMDVFLHELCHIWQGPHDRRFFSHLST 139

Query: 166 LRKECEELMAKGISG 181
           LR     L AKG  G
Sbjct: 140 LRAALIALYAKGYKG 152

BLAST of Lag0037373 vs. ExPASy TrEMBL
Match: A0A6J1FKX5 (uncharacterized protein LOC111446189 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446189 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.4e-82
Identity = 156/185 (84.32%), Postives = 162/185 (87.57%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNA+FYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNASFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPS+ VH SH
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSDTVHSSH 233

BLAST of Lag0037373 vs. ExPASy TrEMBL
Match: A0A6J1IU34 (uncharacterized protein LOC111480590 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480590 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 3.2e-82
Identity = 156/185 (84.32%), Postives = 160/185 (86.49%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNANFYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNANFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIM ALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPSE  H SH
Sbjct: 174 GPNRLGGDSNIMDALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSETAHSSH 233

BLAST of Lag0037373 vs. ExPASy TrEMBL
Match: A0A6J1FJ44 (DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446189 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 1.2e-81
Identity = 155/184 (84.24%), Postives = 161/184 (87.50%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNA+FYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNASFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 273
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPS+ VH SH
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSDTVHSSH 233

BLAST of Lag0037373 vs. ExPASy TrEMBL
Match: A0A6J1J281 (DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480590 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.7e-81
Identity = 155/184 (84.24%), Postives = 159/184 (86.41%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNL  PHNANFYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLISPHNANFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEELMAKGISG+AQGF+LPGRRLG                AAAEGRKRLGSLLPS
Sbjct: 114 ELRKECEELMAKGISGSAQGFELPGRRLGGKSQQPPLSSLRKSALAAAEGRKRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 273
           GPNR GGDSNIM ALSPVQAAAMAAERRLQDDIWCASSQE+PVDEECCSDFPSE  H SH
Sbjct: 174 GPNRLGGDSNIMDALSPVQAAAMAAERRLQDDIWCASSQEMPVDEECCSDFPSETAHSSH 233

BLAST of Lag0037373 vs. ExPASy TrEMBL
Match: A0A0A0K3J6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G161030 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.7e-81
Identity = 153/185 (82.70%), Postives = 159/185 (85.95%), Query Frame = 0

Query: 105 KNPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 164
           KNPALLGLNVGRGIHVKLRLRRPNRD DFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD
Sbjct: 54  KNPALLGLNVGRGIHVKLRLRRPNRDGDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWD 113

Query: 165 ELRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPS 224
           ELRKECEEL+AKG+SGTAQGFDLPGRRLG                AAAEGR+RLGSLLPS
Sbjct: 114 ELRKECEELIAKGVSGTAQGFDLPGRRLGGNVRQPSLSSLRKSSLAAAEGRRRLGSLLPS 173

Query: 225 GPNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCASSQELPVDEECCSDFPSEAVHPSH 274
           GPNR GGDSNIMVALSPVQAAAMAAERRLQDDIWCAS Q +PVDE+CC  FPSEA H S 
Sbjct: 174 GPNRLGGDSNIMVALSPVQAAAMAAERRLQDDIWCASFQGMPVDEDCCPHFPSEAAHSSQ 233

BLAST of Lag0037373 vs. TAIR 10
Match: AT1G55915.1 (zinc ion binding )

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-58
Identity = 114/176 (64.77%), Postives = 129/176 (73.30%), Query Frame = 0

Query: 106 NPALLGLNVGRGIHVKLRLRRPNRDEDFFPFNQVLDTMLHELCHNLHGPHNANFYKLWDE 165
           NP LLG+NV RG+ VKLRLRR N D DF  ++++LDTMLHELCHN HGPHNA+FYKLWDE
Sbjct: 59  NPRLLGVNVNRGVQVKLRLRRVNHDLDFLSYHEILDTMLHELCHNAHGPHNASFYKLWDE 118

Query: 166 LRKECEELMAKGISGTAQGFDLPGRRLG----------------AAAEGRKRLGSLLPSG 225
           LRKECEELM+KGI+GT QGFD+PG+RLG                 AAE R R G+LLPSG
Sbjct: 119 LRKECEELMSKGITGTGQGFDMPGKRLGGLSRQPSLSFLRATAATAAEKRVRAGTLLPSG 178

Query: 226 PNRFGGDSNIMVALSPVQAAAMAAERRLQDDIWCAS-SQELPVDEECCSDFPSEAV 265
           P R GGDS+IM  LSP+QAAAMAAERRL DDIWC S S +   DEE  SD   E V
Sbjct: 179 PQRLGGDSSIMSDLSPIQAAAMAAERRLLDDIWCGSQSTDALEDEENDSDTYKEPV 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023525391.11.0e-8284.86uncharacterized protein LOC111789010 [Cucurbita pepo subsp. pepo][more]
XP_038898345.11.3e-8284.32uncharacterized protein LOC120086023 [Benincasa hispida] >XP_038898346.1 unchara... [more]
KAG6607757.12.9e-8284.32hypothetical protein SDJN03_01099, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022940664.12.9e-8284.32uncharacterized protein LOC111446189 isoform X1 [Cucurbita moschata][more]
KAG7037334.13.8e-8284.32WSS1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
P388381.4e-1535.76DNA-dependent metalloprotease WSS1 OS=Saccharomyces cerevisiae (strain ATCC 2045... [more]
O945802.0e-0936.78DNA-dependent metalloprotease WSS1 homolog 2 OS=Schizosaccharomyces pombe (strai... [more]
Q9P7B51.8e-0537.33DNA-dependent metalloprotease WSS1 homolog OS=Schizosaccharomyces pombe (strain ... [more]
Match NameE-valueIdentityDescription
A0A6J1FKX51.4e-8284.32uncharacterized protein LOC111446189 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IU343.2e-8284.32uncharacterized protein LOC111480590 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FJ441.2e-8184.24DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1J2812.7e-8184.24DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
A0A0A0K3J62.7e-8182.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G161030 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G55915.15.4e-5864.77zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013536WLM domainPFAMPF08325WLMcoord: 105..238
e-value: 1.1E-37
score: 130.0
IPR013536WLM domainPROSITEPS51397WLMcoord: 109..238
score: 27.609325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 263..297
NoneNo IPR availablePANTHERPTHR46622:SF3ZINC ION BINDING PROTEINcoord: 105..278
NoneNo IPR availablePANTHERPTHR46622DNA-DEPENDENT METALLOPROTEASE WSS1coord: 105..278
NoneNo IPR availableCDDcd07344M48_yhfN_likecoord: 143..169
e-value: 0.00323828
score: 34.341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0037373.1Lag0037373.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008237 metallopeptidase activity