Homology
BLAST of Lag0037002 vs. NCBI nr
Match:
XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 847.8 bits (2189), Expect = 4.9e-242
Identity = 412/508 (81.10%), Postives = 454/508 (89.37%), Query Frame = 0
Query: 7 WFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSN 66
WF PFLISL LFT IL+SKLR+INHKKLPPSPPSLPVIGHL LL+D P HR+L NLSN
Sbjct: 8 WFFPFLISL-FLFTFILLSKLRKINHKKLPPSPPSLPVIGHLHLLRD--PLHRVLHNLSN 67
Query: 67 KYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYG 126
KYGPIL LSIGSRPV+VVSSPTAV++CFTKNDIVFANRPRLLSGK++NYNYTA+GFAPYG
Sbjct: 68 KYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYG 127
Query: 127 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNEL 186
HWRNMRRIATTELLSNHRLN YLNIRVQELKLW+KNLY + +FV VEMKS+L EL
Sbjct: 128 QHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKNLYKLICREDNFVGVEMKSKLKEL 187
Query: 187 SFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLE 246
SFN VMRM+SGKRYFG EV D EEA EFRE+ KEL LSGATNPADF ILR+ DFQGL
Sbjct: 188 SFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVFDFQGLN 247
Query: 247 KRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIKG 306
KRMLK S RAD FLQ LIDE RE R SRWPE+K GK+MI++LLSLQESQP+YYSDDIIKG
Sbjct: 248 KRMLKASGRADVFLQKLIDEQRERRLSRWPEDKHGKSMIQTLLSLQESQPDYYSDDIIKG 307
Query: 307 HVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQ 366
HVLTMLAAGTDTT+GTIEW MSLLLNHPTVMKKA EI +C+G+NRMVEEADVS L+Y++
Sbjct: 308 HVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEADVSNLRYME 367
Query: 367 AIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRP 426
AIIYETLR+FP APLLVPHESS+DCRIEGF+VPKGTML+VNAWAIHRDPKVWEDP SFRP
Sbjct: 368 AIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKVWEDPNSFRP 427
Query: 427 ERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEG 486
ERF+NWEGVESYKWIPFGMGRRACPGA LA R+VGLTLATLIQCFEW +VDE+Q+DLSEG
Sbjct: 428 ERFLNWEGVESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVDEQQIDLSEG 487
Query: 487 TGITMPKAEPLEAMCRARSSMLHLLSHL 515
+GITMPKA+ L AMC+ R+SMLHLLS L
Sbjct: 488 SGITMPKAKALVAMCKPRNSMLHLLSCL 512
BLAST of Lag0037002 vs. NCBI nr
Match:
XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-241
Identity = 413/516 (80.04%), Postives = 464/516 (89.92%), Query Frame = 0
Query: 1 MEADT-PWFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHR 60
MEA T W +SLSLLFTLILI KLR+ + +K PPSPPSLP+IGHL LLK PFHR
Sbjct: 20 MEASTITW-----LSLSLLFTLILIHKLRKQSPRKCPPSPPSLPLIGHLHLLK--QPFHR 79
Query: 61 LLQNLSNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTA 120
+LQ+LSNK+GPIL LSIGSRP++V+SSPTAV++CFTKNDI FANRPRLLSGK++NYNYT
Sbjct: 80 VLQDLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTG 139
Query: 121 MGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEM 180
+ FA YGHHWRNMRRIATTELLSNHRLN YLNIRVQELKLW+KNLYGATG+S DFVR+EM
Sbjct: 140 VAFASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFVRLEM 199
Query: 181 KSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRL 240
+S+LNELSFNTVMRM+SGKRYFG +VED EEAREFREIMKEL LSGA+NPADF PILRL
Sbjct: 200 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRL 259
Query: 241 IDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEY 300
D+QGLEKRM+K S RAD FLQNLID RE RAS WPEEK G KTMI+SLLSLQESQP+Y
Sbjct: 260 FDYQGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKHGNKTMIQSLLSLQESQPQY 319
Query: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEAD 360
YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPT+M KA +EI EC+G++R+VEE D
Sbjct: 320 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGD 379
Query: 361 VSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVW 420
VS L+Y++AIIYETLR+FP+APLLVPHESS++CRIEGFD+PKGTML+VNAWAIHRDP++W
Sbjct: 380 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 439
Query: 421 EDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE 480
EDPTSFRPER +NWE ES+KW+PFG+GRRACPGA LAHR+VGLTLATLIQCFEW RV E
Sbjct: 440 EDPTSFRPERLLNWESAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGE 499
Query: 481 EQVDLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
E VDLSEGTGIT+PKA PLEAMCRARSSMLHLLS L
Sbjct: 500 EPVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528
BLAST of Lag0037002 vs. NCBI nr
Match:
XP_022936846.1 (isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 isoflavone 3'-hydroxylase-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 837.8 bits (2163), Expect = 5.0e-239
Identity = 408/516 (79.07%), Postives = 462/516 (89.53%), Query Frame = 0
Query: 1 MEADT-PWFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHR 60
MEA+T W +SLSLLFTL LI KLR+ + ++ PPSPPSLP+IGHL LLK PFHR
Sbjct: 1 MEANTITW-----LSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLK--QPFHR 60
Query: 61 LLQNLSNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTA 120
+LQ+LS+K+GPIL LSIGSRP++V+SSPTAV++CFTKNDI FANRPRLLSGK++NYNYT
Sbjct: 61 VLQDLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTG 120
Query: 121 MGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEM 180
+ A YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLW+KNLYG TG+S DFVR+EM
Sbjct: 121 VAVASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEM 180
Query: 181 KSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRL 240
+S+LNELSFNTVMRM+SGKRYFG +VED EEAREFREIMKEL LSGA+NPADF PILRL
Sbjct: 181 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRL 240
Query: 241 IDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEY 300
D+QGLEKRM+K S RAD FLQNLID RE RASRWPEEK G KTMI+SLLS QESQP+Y
Sbjct: 241 FDYQGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQY 300
Query: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEAD 360
YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPT+M KA +EI EC+G++R+VEE D
Sbjct: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGD 360
Query: 361 VSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVW 420
VS L+Y++AIIYETLR+FP+APLLVPHESS++CRIEGFD+PKGTML+VNAWAIHRDP++W
Sbjct: 361 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 420
Query: 421 EDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE 480
EDPTSFRPERF+NWE ES+KW+PFG+GRRACPG LAHR+VGLTLATLIQCFEW RV E
Sbjct: 421 EDPTSFRPERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGE 480
Query: 481 EQVDLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
E +DLSEGTGITMPKA PL AMCRARSSMLHLLS L
Sbjct: 481 EPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Lag0037002 vs. NCBI nr
Match:
KAG7024282.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 820.1 bits (2117), Expect = 1.1e-233
Identity = 401/516 (77.71%), Postives = 458/516 (88.76%), Query Frame = 0
Query: 1 MEADT-PWFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHR 60
MEA+T W +SLSLLFTL LI KLR+ + ++ PPSPPSLP+IGHL LLK PFHR
Sbjct: 1 MEANTITW-----LSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLK--QPFHR 60
Query: 61 LLQNLSNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTA 120
+LQ+LSNK+GPIL LSIGSRP++V+SSPTAV++CFTKNDI FANRPRLLSG ++NYNYT
Sbjct: 61 VLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIAFANRPRLLSGNYMNYNYTG 120
Query: 121 MGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEM 180
+ A YGHHWRNMRR+AT ELLSN RL+ YLNIRVQELKL +KNLYGATG+S DFVR+EM
Sbjct: 121 VAVASYGHHWRNMRRVATKELLSNQRLDTYLNIRVQELKLCVKNLYGATGESSDFVRLEM 180
Query: 181 KSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRL 240
+S+LNELSFNTVMRM+SGKRYFG +VED EEAREFREIMKEL LSGA+NPADF PILRL
Sbjct: 181 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRL 240
Query: 241 IDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEY 300
D+QGLEKRM+K S+RAD FLQNLID RE R+SRWPEEK G KTMI+SLLS QESQP+Y
Sbjct: 241 FDYQGLEKRMVKASSRADLFLQNLIDCERERRSSRWPEEKHGNKTMIQSLLSFQESQPQY 300
Query: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEAD 360
YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPT+M KA +EI EC+G++R+VEE D
Sbjct: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGD 360
Query: 361 VSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVW 420
VS L+Y++AIIYETLR+FP+APLLVPHESS++CRIEGFD+PKGTML+VNAWAIHRDP++W
Sbjct: 361 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 420
Query: 421 EDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE 480
EDPTSFRPERF+NWE ES+KW+ FG+GR ACPGA LAHR+VGLTLATLIQCFEW RV E
Sbjct: 421 EDPTSFRPERFLNWEAAESHKWVLFGVGRGACPGAALAHRVVGLTLATLIQCFEWQRVGE 480
Query: 481 EQVDLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
E +DLSEGTGITMPKA PL AMCRARSSMLHLLS L
Sbjct: 481 EPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Lag0037002 vs. NCBI nr
Match:
XP_022977005.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 820.1 bits (2117), Expect = 1.1e-233
Identity = 398/503 (79.13%), Postives = 454/503 (90.26%), Query Frame = 0
Query: 13 ISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSNKYGPIL 72
+SLSLL TLILI KLR+ + + PPSPPSLP+IGHL LLK PFHR+LQ+LSNK+GPIL
Sbjct: 20 LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLK--QPFHRVLQDLSNKHGPIL 79
Query: 73 FLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHHWRNM 132
LSIGSRP++V+SSPTAV++CFTKNDI ANRPRLL+GK++NYNYT + FA YGHHWRNM
Sbjct: 80 SLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNM 139
Query: 133 RRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELSFNTVM 192
RRIATTELLSNHRLNAYLNIRVQELKL +KNLYGATG+S DFVR+EM+S+LNELSFN VM
Sbjct: 140 RRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVM 199
Query: 193 RMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKRMLKG 252
RM+SGKRYFG +VED EEAREFREIMKEL L GA+NPADF PILRL D+QGLEKRM++
Sbjct: 200 RMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQA 259
Query: 253 SARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEYYSDDIIKGHVLTM 312
S RAD+FLQNLID R+ RASRWPEEK+G KTMI+SLLSLQESQP++YSDDIIKGHVLTM
Sbjct: 260 SGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTM 319
Query: 313 LAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAIIYE 372
LAAGTDTTTGT+EWAMSLLLNHP +M KA +EI EC+G++R+VEE DVS L+YL AIIYE
Sbjct: 320 LAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYE 379
Query: 373 TLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERFVN 432
TLR+FP APLLVPHESS++CR+EGFD+PKGTML+VNAWAIHRDP+VWEDPTSFRPERF+N
Sbjct: 380 TLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLN 439
Query: 433 WEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTGITM 492
WE ES+KW+PFG+GRRACPGA LAHR+VGLTLATLIQCFEW RV EE +DLS+GTGIT
Sbjct: 440 WETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
Query: 493 PKAEPLEAMCRARSSMLHLLSHL 515
PKA PLEAMCRARSSMLHLLS L
Sbjct: 500 PKAIPLEAMCRARSSMLHLLSPL 519
BLAST of Lag0037002 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 550.1 bits (1416), Expect = 2.7e-155
Identity = 279/502 (55.58%), Postives = 367/502 (73.11%), Query Frame = 0
Query: 12 LISLSLLFTLILISKLRRINHKKLPPSPP-SLPVIGHLLLLKDPSPFHRLLQNLSNKYGP 71
++ LS+ L +S+ RR LPPSP +LPVIGHL L+ HR L +LS KYG
Sbjct: 13 VVLLSISLKLFPVSRRRR----NLPPSPGLALPVIGHLHLI--GKLLHRSLYDLSKKYGS 72
Query: 72 ILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHHWR 131
+ L +G+R VLVVSSP A ++CFTKNDIVFANRP + GK++ YNYT M +PYG HWR
Sbjct: 73 VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 132
Query: 132 NMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELSFNT 191
N+RR+A E+ S LN +L+IR E+K L +LY ++G+ DF +VEMKS+L+ELSFN
Sbjct: 133 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQ--DFGKVEMKSKLSELSFNV 192
Query: 192 VMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKRML 251
MRM++GKRYFG +V D++EA+ FR ++ E+F +GA+NP DF P LR IDF+ EK++
Sbjct: 193 TMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 252
Query: 252 KGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIKGHVLT 311
K S DAFLQ LI E R N K TMI+ LLSLQESQPEYY+D IIKG ++
Sbjct: 253 KISQEMDAFLQRLIHESRIN--------KNNVTMIDHLLSLQESQPEYYTDQIIKGIIMV 312
Query: 312 MLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAIIY 371
+L AGTDT+ T+EWAMSLLLNHP ++KA TEI +G NR++EE D+ KL YL II
Sbjct: 313 LLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIIS 372
Query: 372 ETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERFV 431
ET R+ P AP+LVPHESS DC+++G+DVPKGT+L+VNAWAIHRDP+ W++PT F+PER
Sbjct: 373 ETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHG 432
Query: 432 NWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTGIT 491
E +E K +PFGMGRR+CPG+ LA R+VGLTL LIQCFEW R+ E ++D++EG+G+T
Sbjct: 433 GVE-LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLT 492
Query: 492 MPKAEPLEAMCRARSSMLHLLS 513
MPKA+PLEA+C+ R+ + ++S
Sbjct: 493 MPKAQPLEALCKPRNILHKVVS 496
BLAST of Lag0037002 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 516.9 bits (1330), Expect = 2.6e-145
Identity = 264/495 (53.33%), Postives = 338/495 (68.28%), Query Frame = 0
Query: 12 LISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSNKYGPI 71
L+SLS + T+ ++ K+ K LPP PP++P+IG+L LK P HR LS YG I
Sbjct: 7 LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLK--HPLHRTFTTLSQTYGDI 66
Query: 72 LFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHHWRN 131
L GSR V+VVSSP+ +CFTKNDI+ ANRPR L+GK++ YNYT +G A YG HWRN
Sbjct: 67 FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 126
Query: 132 MRRIATTELLSNHRLNAYLNIRVQEL-KLWLKNLYGATGKSGDFVRVEMKSRLNELSFNT 191
+RRI T ++LSN+RLN++L +R E +L K L + F +VE++ RL E++FN
Sbjct: 127 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 186
Query: 192 VMRMISGKRYFG--AEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKR 251
+MRMISGKRY+G +V D EEA++FREI+ E+ L GA N DF P+LR++D LEKR
Sbjct: 187 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 246
Query: 252 MLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIKGHV 311
+ + R++AFL+ LI+E R G TMI+ LL L ESQPEYYSD +IKG +
Sbjct: 247 CKRIAKRSNAFLEGLIEEHRRGNI-----HSDGGTMIDHLLKLSESQPEYYSDHLIKGLI 306
Query: 312 LTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAI 371
ML AGTDT+ TIEW MS LLNHP V+KKA E+ IG N++V+E D+SKL YLQ I
Sbjct: 307 QGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNI 366
Query: 372 IYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPER 431
I ETLR+ P APLL+PH SS+DC I F+VPK T+++ N W IHRDPK W D SF+PER
Sbjct: 367 ISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER 426
Query: 432 FVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTG 491
F E V K + FG+GRRACPG +LA R VG T+ LIQCFEW R EE++D+ EG G
Sbjct: 427 FEKEEEVN--KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 486
Query: 492 ITMPKAEPLEAMCRA 504
ITMP PL AMC+A
Sbjct: 487 ITMPMKIPLRAMCKA 492
BLAST of Lag0037002 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 505.0 bits (1299), Expect = 1.0e-141
Identity = 263/502 (52.39%), Postives = 334/502 (66.53%), Query Frame = 0
Query: 5 TPWFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNL 64
T ++ +ISL L + + K LPP P LP+IG+L LK P H L
Sbjct: 2 TTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLK--QPLHHTFHTL 61
Query: 65 SNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAP 124
S KYG I L GSR V+VVSS T Q+CFTKNDIV ANRP L+GK++ YN T + +P
Sbjct: 62 SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 121
Query: 125 YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLN 184
YG HWRN+RRI + E+LS+HRLN++L IR E+ ++ L A F VE++ +
Sbjct: 122 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKL--AQKSYNGFTEVELRPMFS 181
Query: 185 ELSFNTVMRMISGKRYFG--AEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDF 244
E++FNT+MRM+SGKRY+G +V D EEAR FR I+KE+ L GA N DF LR DF
Sbjct: 182 EMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDF 241
Query: 245 QGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDD 304
GLEKR+ K S R DAFLQ LIDE R + + TMI+ LL+ Q+SQPEYY+D
Sbjct: 242 DGLEKRLKKISKRTDAFLQGLIDEHRFGK-------RNSNTMIDHLLTQQQSQPEYYTDQ 301
Query: 305 IIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKL 364
IIKG ++ ML AGTDT++ TIEWAMS LLNHP +MKKA E+ IG +R V+E D+SKL
Sbjct: 302 IIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKL 361
Query: 365 QYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPT 424
YLQ+I+YETLR+ APLLVPH SS+D + G+++P+ T+L+VNAW IHRDP +W DPT
Sbjct: 362 PYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPT 421
Query: 425 SFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVD 484
F+PERF EG E K + FG+GRRACPG L+ R GLTL LIQCFEW R+ EE++D
Sbjct: 422 CFKPERFEK-EG-EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKID 481
Query: 485 LSEGTGITMPKAEPLEAMCRAR 505
+ E GIT K L AMC+ R
Sbjct: 482 MVEAKGITAGKKTSLNAMCKVR 490
BLAST of Lag0037002 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 497.3 bits (1279), Expect = 2.1e-139
Identity = 247/499 (49.50%), Postives = 345/499 (69.14%), Query Frame = 0
Query: 8 FSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSNK 67
+S F ++L +F ++ +R K LPP PPSLP+IG+L LK P HR + LS K
Sbjct: 9 YSVFYLALFFIFNIV----IRARKFKNLPPGPPSLPIIGNLHHLK--RPLHRTFKGLSEK 68
Query: 68 YGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGH 127
YG + L GSR V+VVSS + Q CFTKND+V ANRPR LSGK++ YNYT +G YG
Sbjct: 69 YGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGE 128
Query: 128 HWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELS 187
HWRN+RRI ++LSNHR+N++ IR E + + L A S +F +E+ SRL +++
Sbjct: 129 HWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRL--ADDSSTNFAEMELSSRLYDMT 188
Query: 188 FNTVMRMISGKRYFG--AEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGL 247
FN +MRMISGKRY+G + D +EA +FR+++ EL LSGA N DF P+LR +DF+ L
Sbjct: 189 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 248
Query: 248 EKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIK 307
EKR+ S + DAFL+ LI+E R ++++ TMI+ LL+LQ+SQPEYY+D IIK
Sbjct: 249 EKRLKDISGKTDAFLRGLIEEHR-------TKKERANTMIDHLLNLQDSQPEYYTDQIIK 308
Query: 308 GHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYL 367
G L ML AGTD++ T+EW+MS LLNHP V+KK E+ +G +R+V+E+D+ KL YL
Sbjct: 309 GLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYL 368
Query: 368 QAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFR 427
+ +I ETLR++ APLL+PH +S +C I G+ VP+ T++++NAWAIHRDP++W + T+F+
Sbjct: 369 KNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFK 428
Query: 428 PERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSE 487
PERF +E K I FGMGRRACPG LA R + +TLA LIQCF+W ++ +++DL+E
Sbjct: 429 PERFEKKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 488
Query: 488 GTGITMPKAEPLEAMCRAR 505
G T+ K PL+AMC++R
Sbjct: 489 RDGFTLTKLVPLKAMCKSR 490
BLAST of Lag0037002 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 482.3 bits (1240), Expect = 7.0e-135
Identity = 239/495 (48.28%), Postives = 341/495 (68.89%), Query Frame = 0
Query: 12 LISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSNKYGPI 71
L LS+ F + L+ + R+ K LPP P SLP+IG+L LK P +R + L+ KYG +
Sbjct: 11 LFYLSIFFIIRLLFQSRKF--KNLPPGPTSLPIIGNLHHLK--RPLNRTFKALTEKYGNV 70
Query: 72 LFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHHWRN 131
+ L GSR V+VVSS + Q+CFTKND+V ANRPR LSGK++ YNYT +G YG HWRN
Sbjct: 71 ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 130
Query: 132 MRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELSFNTV 191
+RRI + ++LSNHR+N + IR E + +K L A S F VE+ R +++FN +
Sbjct: 131 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKL--AEDSSTKFAEVELTFRFFDMTFNNI 190
Query: 192 MRMISGKRYFG--AEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKRM 251
MRMISGKRY+G ++ + +EA +FR+++ EL LSGA N DF P+L+ +DF+ LEKR+
Sbjct: 191 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 250
Query: 252 LKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIKGHVL 311
+ + D FL L+ E R ++++ TMI+ LL++QESQPEYY+D IIKG L
Sbjct: 251 KRIGEKNDVFLSGLLQEQRS-------KKERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 310
Query: 312 TMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAII 371
ML AGTD++ T+EW MS +LN+P V+KK E+ +G +R+V+E+D+ KL YL+ +I
Sbjct: 311 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 370
Query: 372 YETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERF 431
YETLR++ APLL+PH ++ +C + G+ VP+ T++++NAWAIHRDP+ W + T+F+PERF
Sbjct: 371 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 430
Query: 432 VNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTGI 491
+E K I FGMGRRACPG LA R + +TLA L+QCF+W R+++E++D+SE G
Sbjct: 431 DKKGELE--KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGF 490
Query: 492 TMPKAEPLEAMCRAR 505
TM K PL+AMC+ R
Sbjct: 491 TMTKLLPLKAMCKTR 490
BLAST of Lag0037002 vs. ExPASy TrEMBL
Match:
A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)
HSP 1 Score: 837.8 bits (2163), Expect = 2.4e-239
Identity = 408/516 (79.07%), Postives = 462/516 (89.53%), Query Frame = 0
Query: 1 MEADT-PWFSPFLISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHR 60
MEA+T W +SLSLLFTL LI KLR+ + ++ PPSPPSLP+IGHL LLK PFHR
Sbjct: 1 MEANTITW-----LSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLK--QPFHR 60
Query: 61 LLQNLSNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTA 120
+LQ+LS+K+GPIL LSIGSRP++V+SSPTAV++CFTKNDI FANRPRLLSGK++NYNYT
Sbjct: 61 VLQDLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTG 120
Query: 121 MGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEM 180
+ A YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLW+KNLYG TG+S DFVR+EM
Sbjct: 121 VAVASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEM 180
Query: 181 KSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRL 240
+S+LNELSFNTVMRM+SGKRYFG +VED EEAREFREIMKEL LSGA+NPADF PILRL
Sbjct: 181 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRL 240
Query: 241 IDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEY 300
D+QGLEKRM+K S RAD FLQNLID RE RASRWPEEK G KTMI+SLLS QESQP+Y
Sbjct: 241 FDYQGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQY 300
Query: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEAD 360
YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPT+M KA +EI EC+G++R+VEE D
Sbjct: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGD 360
Query: 361 VSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVW 420
VS L+Y++AIIYETLR+FP+APLLVPHESS++CRIEGFD+PKGTML+VNAWAIHRDP++W
Sbjct: 361 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 420
Query: 421 EDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE 480
EDPTSFRPERF+NWE ES+KW+PFG+GRRACPG LAHR+VGLTLATLIQCFEW RV E
Sbjct: 421 EDPTSFRPERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGE 480
Query: 481 EQVDLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
E +DLSEGTGITMPKA PL AMCRARSSMLHLLS L
Sbjct: 481 EPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509
BLAST of Lag0037002 vs. ExPASy TrEMBL
Match:
A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)
HSP 1 Score: 820.1 bits (2117), Expect = 5.3e-234
Identity = 398/503 (79.13%), Postives = 454/503 (90.26%), Query Frame = 0
Query: 13 ISLSLLFTLILISKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLSNKYGPIL 72
+SLSLL TLILI KLR+ + + PPSPPSLP+IGHL LLK PFHR+LQ+LSNK+GPIL
Sbjct: 20 LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLK--QPFHRVLQDLSNKHGPIL 79
Query: 73 FLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHHWRNM 132
LSIGSRP++V+SSPTAV++CFTKNDI ANRPRLL+GK++NYNYT + FA YGHHWRNM
Sbjct: 80 SLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNM 139
Query: 133 RRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELSFNTVM 192
RRIATTELLSNHRLNAYLNIRVQELKL +KNLYGATG+S DFVR+EM+S+LNELSFN VM
Sbjct: 140 RRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVM 199
Query: 193 RMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKRMLKG 252
RM+SGKRYFG +VED EEAREFREIMKEL L GA+NPADF PILRL D+QGLEKRM++
Sbjct: 200 RMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQA 259
Query: 253 SARADAFLQNLIDELRENRASRWPEEKQG-KTMIESLLSLQESQPEYYSDDIIKGHVLTM 312
S RAD+FLQNLID R+ RASRWPEEK+G KTMI+SLLSLQESQP++YSDDIIKGHVLTM
Sbjct: 260 SGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTM 319
Query: 313 LAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAIIYE 372
LAAGTDTTTGT+EWAMSLLLNHP +M KA +EI EC+G++R+VEE DVS L+YL AIIYE
Sbjct: 320 LAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYE 379
Query: 373 TLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERFVN 432
TLR+FP APLLVPHESS++CR+EGFD+PKGTML+VNAWAIHRDP+VWEDPTSFRPERF+N
Sbjct: 380 TLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLN 439
Query: 433 WEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTGITM 492
WE ES+KW+PFG+GRRACPGA LAHR+VGLTLATLIQCFEW RV EE +DLS+GTGIT
Sbjct: 440 WETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
Query: 493 PKAEPLEAMCRARSSMLHLLSHL 515
PKA PLEAMCRARSSMLHLLS L
Sbjct: 500 PKAIPLEAMCRARSSMLHLLSPL 519
BLAST of Lag0037002 vs. ExPASy TrEMBL
Match:
A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)
HSP 1 Score: 806.6 bits (2082), Expect = 6.0e-230
Identity = 397/513 (77.39%), Postives = 449/513 (87.52%), Query Frame = 0
Query: 7 WFSPFLISLSLLFTLILISKLRRI-NHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLS 66
WFS +SL L+ TLIL+SKLR++ N KKLPP+PPSLP+IGHL LLK+ PFHR+L NLS
Sbjct: 16 WFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKE--PFHRVLHNLS 75
Query: 67 NKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPY 126
NKYGPIL L+IGSRPVLVVSSPTAV++CFTKNDIVFANRPRL+SGK++NYNYTA+GFAPY
Sbjct: 76 NKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPY 135
Query: 127 GHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNE 186
G HWRNMRRIATTELLSNHRLN YLNIRV+ELKLW+KN G GDFV V+MKS+L E
Sbjct: 136 GQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKE 195
Query: 187 LSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGL 246
LSFNTVMRMISGKRYFG EVED EEA EFREIMKEL LSGATN ADF ILR+ D +G+
Sbjct: 196 LSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGV 255
Query: 247 EKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGK---TMIESLLSLQESQPEYYSDD 306
+KRM+K S RAD FLQ LIDE R+ R SRWPEEKQ + +MI +LLSLQESQP+YYSDD
Sbjct: 256 KKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDD 315
Query: 307 IIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKL 366
IIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP MKKA EI +C+G+N+MVEE DVS+L
Sbjct: 316 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSEL 375
Query: 367 QYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPT 426
+YL+AIIYETLRMFP APLLVPHE S+DC IEGF+VPKGTML+VNAW IHRDPKVWEDPT
Sbjct: 376 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPT 435
Query: 427 SFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE-EQV 486
SF+PERF+NWEGVE+YKWIPFGMGRRACPG LA R +GLTLATLIQCFEW +VDE EQ+
Sbjct: 436 SFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQI 495
Query: 487 DLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
DLSEG+GITMPKA+ LEAMC+ R+SMLHLL+ L
Sbjct: 496 DLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523
BLAST of Lag0037002 vs. ExPASy TrEMBL
Match:
A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)
HSP 1 Score: 798.1 bits (2060), Expect = 2.1e-227
Identity = 395/513 (77.00%), Postives = 448/513 (87.33%), Query Frame = 0
Query: 7 WFSPFLISLSLLFTLILISKLRRI-NHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLS 66
WFS +SL L+ TLIL+SKLR++ N KKLPP+PPSLP+IGHL LLK+ PFHR+L NLS
Sbjct: 13 WFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKE--PFHRVLHNLS 72
Query: 67 NKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPY 126
NKYGPIL L+IGSRPVLVVSSPTAV++CFTKNDIVFANRPRL+SGK++NYNYTA+GFAPY
Sbjct: 73 NKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAVGFAPY 132
Query: 127 GHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNE 186
G HWRNMRRIATTELLSNHRLN YLNIRV+ELKLW+KN G GDFV V+MKS+L E
Sbjct: 133 GQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDMKSKLKE 192
Query: 187 LSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGL 246
LSFNTVMRMISGKRYFG EVED EEA EFREIMKEL LSGATN ADF ILR+ D +G+
Sbjct: 193 LSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRVFDLEGV 252
Query: 247 EKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGK---TMIESLLSLQESQPEYYSDD 306
+KRM+K S RAD FLQ LIDE R+ + RWPEEKQ + +MI +LLSLQESQP+YYSDD
Sbjct: 253 KKRMMKASGRADVFLQKLIDEERK-KEFRWPEEKQKQAKTSMIRTLLSLQESQPQYYSDD 312
Query: 307 IIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKL 366
IIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHP MKKA EI +C+G+N+MVEE DVS+L
Sbjct: 313 IIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEEGDVSEL 372
Query: 367 QYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPT 426
+YL+AIIYETLRMFP APLLVPHE S+DC IEGF+VPKGTML+VNAW IHRDPKVWEDPT
Sbjct: 373 KYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPKVWEDPT 432
Query: 427 SFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDE-EQV 486
SF+PERF+NWEGVE+YKWIPFGMGRRACPG LA R +GLTLATLIQCFEW +VDE EQ+
Sbjct: 433 SFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKVDEQEQI 492
Query: 487 DLSEGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
DLSEG+GITMPKA+ LEAMC+ R+SMLHLL+ L
Sbjct: 493 DLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519
BLAST of Lag0037002 vs. ExPASy TrEMBL
Match:
A0A6J1C4W6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE=3 SV=1)
HSP 1 Score: 672.2 bits (1733), Expect = 1.8e-189
Identity = 330/510 (64.71%), Postives = 408/510 (80.00%), Query Frame = 0
Query: 8 FSPFLISLSLLFTLILI--SKLRRINHKKLPPSPPSLPVIGHLLLLKDPSPFHRLLQNLS 67
FS FL+ L+LLF LI + K ++++ K LPP PPSLPVIGHL LL++ PFH++LQNLS
Sbjct: 7 FSRFLVPLALLFALIHVFNFKFKKVSSKNLPPXPPSLPVIGHLHLLRE--PFHQMLQNLS 66
Query: 68 NKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPY 127
+YGPIL LS+G R V+VVSSP A Q+CFTKNDIVFANRPR++SGK +NYNYT +G Y
Sbjct: 67 QRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTTVGATAY 126
Query: 128 GHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNE 187
G HWR MRRIATTELLS HRL +++N+R +E+KLWLKNLY TG++ VRVE++SRL E
Sbjct: 127 GQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNN--VRVEIRSRLTE 186
Query: 188 LSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGL 247
LSFN V+RM++GKRYFG EV+D+EE RE +++MKE+ LSG + PADF +LRLID+QG
Sbjct: 187 LSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLIDYQGF 246
Query: 248 EKRMLKGSARADAFLQNLIDELRENRAS-RWPEEKQGKTMIESLLSLQESQPEYYSDDII 307
KR+ K A AD F+Q LID RE +AS + E+ K MI+++LSLQ+S P+YY D II
Sbjct: 247 RKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYKDHII 306
Query: 308 KGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQY 367
KG +LT+L AGTDTT GTIEW MSLLLNHPT+MKKA +EI++ +G+ R+VEEAD++ L Y
Sbjct: 307 KGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLANLPY 366
Query: 368 LQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSF 427
LQAII ET R+FP P+LVP ESS+DC I G+DVPKGTMLVVN WAIHRDP+VW+DPT F
Sbjct: 367 LQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQVWQDPTRF 426
Query: 428 RPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLS 487
+PERF N E VE K +PFGMGRRACPGA LAHR+VGLTLATLIQCFEW R+D ++DL
Sbjct: 427 QPERFENGEVVEVNKLLPFGMGRRACPGAGLAHRVVGLTLATLIQCFEWDRIDPNEIDLX 486
Query: 488 EGTGITMPKAEPLEAMCRARSSMLHLLSHL 515
+G G+TMPKA PLEAMC+ R M+ LLSHL
Sbjct: 487 QGNGLTMPKAHPLEAMCKPRGPMISLLSHL 512
BLAST of Lag0037002 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 515.4 bits (1326), Expect = 5.3e-146
Identity = 265/515 (51.46%), Postives = 369/515 (71.65%), Query Frame = 0
Query: 1 MEADTPWFSPFLISLSLLFTLILISKLRRINHKKLPPSPP-SLPVIGHLLLLKDP--SPF 60
ME T FS + LSL++ LI KL+R LPPSP SLPVIGHL LLK P F
Sbjct: 1 METKTLIFSILFVVLSLIY---LIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTF 60
Query: 61 HRLLQNLSNKYGPILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNY 120
L Q+L+N PI L +G+R V V SS + ++CFTKND+V ANRP + KHV Y+Y
Sbjct: 61 LSLSQSLNN--APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDY 120
Query: 121 TAMGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRV 180
T M A YG HWRN+RRI + E+ SNHRLN++L+IR E++ + L + S +FV+V
Sbjct: 121 TTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL--SRNFSQEFVKV 180
Query: 181 EMKSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPIL 240
+MKS L++L+FN ++RM++GKRY+G VED EA+ R+++ ++ +GA N D+ P+L
Sbjct: 181 DMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVL 240
Query: 241 RLIDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPE 300
RL+ E R+ K + R D FLQ L+DE RE +++G TMI+ LL+LQESQP+
Sbjct: 241 RLV--SDYETRVKKLAGRLDEFLQGLVDEKRE-------AKEKGNTMIDHLLTLQESQPD 300
Query: 301 YYSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEA 360
Y++D IIKG++L ++ AGTDT+ T+EWA+S +LNHP V+ KA EI IG +R+++E+
Sbjct: 301 YFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDES 360
Query: 361 DVSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKV 420
D+S L YLQ I+ ETLR++P AP+L+PH +S+DC++ G+D+P+GT+L+ N WAIHRDP++
Sbjct: 361 DISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQL 420
Query: 421 WEDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVD 480
W+DP SF+PERF EG E+ K +PFG+GRRACPG+ LAHRL+ LTL +LIQC EW ++
Sbjct: 421 WDDPMSFKPERFEK-EG-EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG 480
Query: 481 EEQVDLSEGTGITMPKAEPLEAMCRARSSMLHLLS 513
EE VD+SEG G+TMPKA+PLEAMCRAR S++ + +
Sbjct: 481 EE-VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFN 494
BLAST of Lag0037002 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 260/508 (51.18%), Postives = 353/508 (69.49%), Query Frame = 0
Query: 1 MEADTPWFSPFLISLSLLFTLILISKLRRINHKKLPPSPP-SLPVIGHLLLLKDPSPFHR 60
ME T F+ ISLSL F +I +++R LPPSP +LPVIGHL LLK P HR
Sbjct: 1 MEGQTLIFTFLFISLSLTF---IIGRIKR--RPNLPPSPSWALPVIGHLRLLK--PPLHR 60
Query: 61 LLQNLSNKYG--PILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNY 120
+ ++S G PI+ L +G+R V VVSS + ++CFTKND+V ANR L+ KH++Y
Sbjct: 61 VFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGC 120
Query: 121 TAMGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRV 180
T + A YG HWRN+RRI E+ S HRLN++ +IR E+ + L + S +F +V
Sbjct: 121 TTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACL--SRNSSLEFTKV 180
Query: 181 EMKSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPIL 240
EMKS + L+FN ++RM++GK Y+G ED EA+ RE++ E G GA N AD+ PIL
Sbjct: 181 EMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPIL 240
Query: 241 RLIDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPE 300
I G EKR+ K ++R D FLQ L+DE RE + EK+ TM++ LL LQE+QPE
Sbjct: 241 TWI--TGSEKRIKKIASRLDEFLQGLVDERREGK------EKRQNTMVDHLLCLQETQPE 300
Query: 301 YYSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEA 360
YY+D+IIKG +L+++ AGTDT+ T+EW +S LLNHP ++ KA EI +G NR+VEE+
Sbjct: 301 YYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEES 360
Query: 361 DVSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKV 420
D+S L YLQ I+ E+LR++P +PLLVPH +S+DC++ G+ +P+GTML+ NAWAIHRDPK+
Sbjct: 361 DLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKI 420
Query: 421 WEDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVD 480
W+DPTSF+PERF EG E+ K + FG+GRRACPG+ LA RL LT+ +LIQCFEW R+
Sbjct: 421 WDDPTSFKPERFEK-EG-EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIG 480
Query: 481 EEQVDLSE-GTGITMPKAEPLEAMCRAR 505
EE+VD++E G G+ MPKA PL AMC+AR
Sbjct: 481 EEEVDMTEGGGGVIMPKAIPLVAMCKAR 489
BLAST of Lag0037002 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 490.3 bits (1261), Expect = 1.8e-138
Identity = 257/507 (50.69%), Postives = 350/507 (69.03%), Query Frame = 0
Query: 1 MEADTPWFSPFLISLSLLFTLILISKLRRINHKKLPPSPP-SLPVIGHLLLLKDPSPFHR 60
MEA F+ I LSL+F LI +++R LPPSP +LPVIGHL LLK P HR
Sbjct: 1 MEALMLIFTFCFIVLSLIF---LIGRIKR--KLNLPPSPAWALPVIGHLRLLK--PPLHR 60
Query: 61 LLQNLSNKYG--PILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNY 120
+ ++S G PI+ L +G+R + VVSS + ++CFTKND++ ANR +S KH++Y
Sbjct: 61 VFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGN 120
Query: 121 TAMGFAPYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRV 180
+ + A Y HWRN+RRI E+ S HRLN++ +IR E++ + L S F +V
Sbjct: 121 STVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRL--LRNSSYGFTKV 180
Query: 181 EMKSRLNELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPIL 240
EMKS ++L+FN ++RM++GK Y+G ED EA+ R ++ E SG N AD+ PIL
Sbjct: 181 EMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPIL 240
Query: 241 RLIDFQGLEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPE 300
I + E R+ K + R D FLQ L+DE RE + EK+ TM++ LL LQE+QPE
Sbjct: 241 TWITYS--ETRIKKLAGRLDEFLQGLVDEKREGK------EKKENTMVDHLLCLQETQPE 300
Query: 301 YYSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEA 360
YY D IIKG +L+++A GTDTT T+EWA+S LLN+P V+ KA EI IG +R++EE+
Sbjct: 301 YYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEES 360
Query: 361 DVSKLQYLQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKV 420
D+ L YLQ I+ ETLR++P AP+L+PH +S+DC++ G+D+P+GTML+ NAWAIHRDP +
Sbjct: 361 DIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLL 420
Query: 421 WEDPTSFRPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVD 480
W+DPTSF+PERF EG E+ K +PFG+GRRACPG+ LA RLV L+L +LIQCFEW R+
Sbjct: 421 WDDPTSFKPERFEK-EG-EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIG 480
Query: 481 EEQVDLSEGTGITMPKAEPLEAMCRAR 505
EE+VD++EG G+TMPKA PLEAMCRAR
Sbjct: 481 EEEVDMTEGPGLTMPKARPLEAMCRAR 488
BLAST of Lag0037002 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 488.0 bits (1255), Expect = 9.1e-138
Identity = 254/497 (51.11%), Postives = 340/497 (68.41%), Query Frame = 0
Query: 12 LISLSLLFTLILISKLRRINHKKLPPSPP-SLPVIGHLLLLKDPSPFHRLLQNLSNKYG- 71
++S LFTL + R LPPSP SLPVIGHL LLK P HR +LS G
Sbjct: 8 ILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLK--PPLHRTFLSLSKSIGN 67
Query: 72 -PILFLSIGSRPVLVVSSPTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAPYGHH 131
P+ L +G+R V V+SS + ++CFTKND+V ANRP+ KH+ YN T + A YG H
Sbjct: 68 APVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDH 127
Query: 132 WRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLNELSF 191
WRN+RRIA E+ S HRLN++L IR E++ + +L + FV VEMK+ L L+
Sbjct: 128 WRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHL--SRDSLHGFVEVEMKTLLTNLAS 187
Query: 192 NTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQGLEKR 251
NT +RM++GKRYFG ED ++A+ + ++ E +GA NP D+ ILR + EKR
Sbjct: 188 NTTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKR 247
Query: 252 MLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDIIKGHV 311
+ R D FLQ L+DE R E+++G+TMI+ LL+LQ+ QP+YY+D IIKG +
Sbjct: 248 IKNLGNRFDTFLQKLVDEKR-------AEKEKGETMIDHLLALQDIQPDYYTDVIIKGII 307
Query: 312 LTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQYLQAI 371
LT++ AGTDT++ T+EWAMS LLNHP ++KKA EI E +G +R+V+E+D+ L YLQ+I
Sbjct: 308 LTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSI 367
Query: 372 IYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSFRPER 431
+ ETLRM+P PLL+PH SS+DC++ G+D+P GTM++ NAWA+HRDP+VWEDP F+PER
Sbjct: 368 VLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER 427
Query: 432 FVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLSEGTG 491
F EG E+ K I FGMGRRACPGA LAHRL+ L +L+QCFEW RV E+ VD++E G
Sbjct: 428 FEK-EG-EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 486
Query: 492 ITMPKAEPLEAMCRARS 506
T+PKA PL AMC+ARS
Sbjct: 488 ATLPKAIPLRAMCKARS 486
BLAST of Lag0037002 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 475.3 bits (1222), Expect = 6.1e-134
Identity = 250/508 (49.21%), Postives = 338/508 (66.54%), Query Frame = 0
Query: 12 LISLSLLFTLILISKLRRINHKKLPPSPPS-LPVIGHLLLLKDPSPFHRLLQNLS----- 71
L S+ L LI+ K + + LPPSPP LP+IGHL LLK P HR L++ S
Sbjct: 10 LYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLK--PPIHRTLRSFSETLDH 69
Query: 72 NKYGPILFLSIGSRPVLVVSS-PTAVQDCFTKNDIVFANRPRLLSGKHVNYNYTAMGFAP 131
N G ++ L +GSR V VVSS A ++CF KND+V ANRP+++ GKHV YN T M AP
Sbjct: 70 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 129
Query: 132 YGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWLKNLYGATGKSGDFVRVEMKSRLN 191
YG HWRN+RR+ T E+ S HRLN +L +R E++ + L G VE+K L
Sbjct: 130 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTV--VELKPMLM 189
Query: 192 ELSFNTVMRMISGKRYFGAEVEDAEEAREFREIMKELFGLSGATNPADFFPILRLIDFQG 251
+L+FN +MRM++GKRY+G E D EEA+ R+++ ++ + + N D+ PILRL F
Sbjct: 190 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSS 249
Query: 252 LEKRMLKGSARADAFLQNLIDELRENRASRWPEEKQGKTMIESLLSLQESQPEYYSDDII 311
E R+ K D FLQ LID+ R +++ G TMI+ LL LQ+S EYY+D II
Sbjct: 250 YENRVKKLGEETDKFLQGLIDDKR-------GQQETGTTMIDHLLVLQKSDIEYYTDQII 309
Query: 312 KGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTVMKKACTEISECIGDNRMVEEADVSKLQY 371
KG +L M+ AGT+T+ T+EWA+S LLNHP V+ KA EI +G +R++EEAD+S+L Y
Sbjct: 310 KGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPY 369
Query: 372 LQAIIYETLRMFPIAPLLVPHESSQDCRIEGFDVPKGTMLVVNAWAIHRDPKVWEDPTSF 431
L+ I+ ETLR+ P PLLVPH +S+DC+I +D+P+GT L+VNAWAIHRDP W+DP SF
Sbjct: 370 LKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSF 429
Query: 432 RPERFVNWEGVESYKWIPFGMGRRACPGATLAHRLVGLTLATLIQCFEWGRVDEEQVDLS 491
+PERF E E+ K + FG+GRRACPG+ LA R+VGL L +LIQCFEW RV +VD+
Sbjct: 430 KPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMK 489
Query: 492 EGTGITMPKAEPLEAMCRARSSMLHLLS 513
EG G T+PKA PL+A+C+AR + ++S
Sbjct: 490 EGVGNTVPKAIPLKAICKARPFLHKIIS 502
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038896686.1 | 4.9e-242 | 81.10 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_023535658.1 | 1.8e-241 | 80.04 | isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022936846.1 | 5.0e-239 | 79.07 | isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 i... | [more] |
KAG7024282.1 | 1.1e-233 | 77.71 | Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022977005.1 | 1.1e-233 | 79.13 | isoflavone 3'-hydroxylase-like [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 2.7e-155 | 55.58 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ9 | 2.6e-145 | 53.33 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 1.0e-141 | 52.39 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
P93147 | 2.1e-139 | 49.50 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNR0 | 7.0e-135 | 48.28 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F8L9 | 2.4e-239 | 79.07 | isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
A0A6J1IL26 | 5.3e-234 | 79.13 | isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ... | [more] |
A0A5A7VB12 | 6.0e-230 | 77.39 | Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... | [more] |
A0A1S3BWG9 | 2.1e-227 | 77.00 | LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103... | [more] |
A0A6J1C4W6 | 1.8e-189 | 64.71 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 5.3e-146 | 51.46 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37340.1 | 1.3e-139 | 51.18 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
AT4G37360.1 | 1.8e-138 | 50.69 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37330.1 | 9.1e-138 | 51.11 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT5G36220.1 | 6.1e-134 | 49.21 | cytochrome p450 81d1 | [more] |