Lag0036984 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036984
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease H-like superfamily protein
Location	chr2: 2607807 .. 2611220 (-)
RNA-Seq Expression	Lag0036984
Synteny	Lag0036984

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAACTTAATGAGCCATCATGTACAAGTCAATTTAAGTTGCCCTGTATGTCATGACGAGACCGAAACCACTAATCATGCACTATTTCGATGCCCCAGAGCCCGGGAGATATGGATGATTCTAATGCCAACCATATCGATGGATCAGTGTGATAGAATGGAGATTAAAGATCGCTGGTTAAGTTTTGCAGACTTTGCAAACCAGATTTTAGAATGCATTAGTGTGGGTGCATGGGCAATTTGGAATGACAGGAATAACCTAATCCGGGACTTACCAATTCCAAATTCTTCGGTTAAAAGTGAGTGGATTCAGGAATATTTATCAGAGTATTGGGCGGCCAATTCGATGGGAAGAGCGGTGGTACAGTCGAATGAAGATGTTAACACCATTATTTCGACAGGTGGAGATTTTGTCATGAGTGTTGATGCGTCTTTTCAGGATGATACGTCCAAATGCGGCATCGGTATGGTTCTGCGCACTAAACAAGGCAATGTTCTGGTTGCCCAGAATTTTCATGTTTCAGGCTGTCAATTCCCTCTGAGTGCTGAAGCTGTCGCGGTACTGTCAGGGCTGCAATTAGCGAGGAACTTGAAGGTTCGGAGTTTAACAGTCCTCTCTGACTGTTTGGCTCTAATACAGTCTATAAATGGTCGTATACAAGGACAATCAAGTATAGCTACGATATTATGGGACATTAAAGCAATTCAAGACTCCTTTGAGTTAGTCAATTTTAAGTTTGTTAGTCGTTGTTATAATTATTCACCCATAACATGGCACGAGCGGGTTGTTTGGAACCACCATGTTTATGGTTAGGAAACTTTCCTGAGTGGATGGAAAGGGTGTCGCTCCAGGAGCGTCATTTGTTTGTACCCATGAGAATTTCTTGATTATGAAATAGTCTATTTTATCAAAAAAAAAAAAAAAAGTTGATGATTCGATCTCTATGCATAATTGTTGAACTCAAAAAAATTATTCATTTTAGAGTAAAATTTTCTGATGAAGTTTAGTATAAAAATTGATATTTTTTTTTTGGGAATAATAAAAATTGATATTTTCTAGAGAAAAAACAGCATTCTTGAAATAATTTTGAAAATATGATTTAAATAAAATTCCAATTTCGATTCCTCTACGACATATCGACTGAGAGAGAGCCCTGCAGGTGCTTATCAGAATCTGCTAGAACCATCTGGCGATGCTGTAATTTAGAAGCATATTCGTTAAAAAGCCGATAATCAAAAAGCAAGACGAAAGCCAAAGGGAAAATAAAATCTGTGCCCTGGATTTCTCATGTATAAAGCAAGACCTCAAGGTCAATTTCCCGATGATCCCATGGACTGTACAAAGTCGGACATGATGGAGGCTGAATTTGCGTCGAACAGAGCCGTCGAAGGCGATTCTGTCCGCGAATTACTCACATTGGCTCGTCAATTCATCAATCAACGAAAGCCTTCCCAAGCCCTCCAAGCGGTATGTTTAACAAAAAGTTCATCTATCTTGGATCTTGTTCTCATCGCTCATAACCTTTCTTGGTCTATTTTCGTTCTTTCAAAACCTTTCCGTGATTGCTTATTTATATTTAGCTTCTGGGTTTTCTTTCAACTATAGATTGTCGAGTTTCTGTAAATTAAATTACTTCAGTTTAGGTCCGGTTGCCCAATGGGATAGAACATTCACCAGTAGGGCTTTCCTCCTCGCTTGACACATTGGGAAAAGACTTCAATTTCTTGTAGAGCCGAAAATTCTGTTGATAATCGTCATATGTACATTTCTAGTAATTGGGCTATGCTGTTATTTTTTTAATAAATTCCTAAAATACCTGCTCAAGAGTCCCCTTTTCGACATCCCAATTTTCTTTGAAGCAAGATAAGAAAATTCCATCACTTCGTGGAGTTTTTTTTTTTTTTTTTTGAAGTACTTCTTGGAGCTTCTTATTCTCAGTGTTAAGTACAGTTTGCTTGATTTCCATCTTTGAGAATAGAAACCTGAAGTTAGAGTGATACATCTTTCATAGTAATAATAACACAGAGGAATATCTGAAGCTTAAAAAGAGGGCAATTTGTAAAATACAAACCCATTCACACACACAAGAAGGAAAAAAGAAAAGGGGAGAAGCATCAGAATGGAGAGCACAAGTGCCTGAAAGAAGAGGAAACATTTTAAAATGCTCCATTTAGTGGTCGTACTTGAAATAAATAAATTGAAAAAAGAAACTAGTTCCCAATTATATATATAACAAGTCTAGCGGCCAATAACTTCGCTGTTATTTCGTACAGCACTGACAAAAATAATACCGCTACAGACCCCAGTGAGGAGTAGACTTTTTGGGAATCAAAGTAGTAATAGCCTCGATAGTGATTCTCACAGAATTCTTAGAATTCCTCCTTAAGAGTCCCCTTTCATACATTTCTATTTTCTTTCAAGCAAGCCTATAAGAAACAAGCTACTAAGTCCTGCCGATCTTATGAGAGTTTGAAATGCCGCCACTTCCTTCTTTCGATGTTTGTCATTGATATTTTGTGTGCTTGAATGATTGTATTAATCAAGTTGTAGTTAAATTTCATTCGGCATTCTGAACAATTGCATCATAAATCTGTCAGCACGAGCAGATTGCAGTGGTCTACATATTTGACATTCAATCCATGACTACTGAATCTTTCTCAGGAATAAAAACCTACACTTGGGTTATATTTGTCATCGTTGTTTCAATGAACATACACAGAATTCTATTATAGAATTTGAAATGTTTTAGAACTTTCTACCCATTGACTTGTACATATATTTATCAATTGTATTACTTTCCTTTTTGTTAGTACCAATTGTGTATTAATTATTATATACCATAAGCTCTCTACCCCTTGCTGAGTGAGCCAGATATTGATAAATGTTTTATAGGTTTAAGAGGAAAGGATAATACACATGCACGTAGTTGTAGTAGTCAGTTCAGATGCCTCAATCAACATGGCTAAATCATGGATGAAGCGAATGAGCAAATGAAAACAACAAATTTTGTTAATAGCTTTCAGCACCTTGTTTTTCATGTATGAACAGGTTGTAATGGCAATGAGAACCCAAGGTGGGGATGCAGCAGTCTTTCAATCTTTGCACCGTGCCCGTGAGCTGTACAGAACTCGATTACAGGAGACTGCTGATGTTGATCAACTAGCTTCTTTATTTGCAGAGTGTGCTATAGCTGAAGCCCAGCCTCTGAACCCTGAACCTGCAGGAAGTAACATATCTGGTTCAGCAGTTGTCGAGGCTCACGGTAATTCTATACTGGCAGAAACTGGTAGGATGCAAGTTGTTATGGACGCCTTCTCAGATGGGAGCAGTTTTATTTGTTTGCAATGTGGGGGTCTTGTTAGTAACCATCGCAAAGATGAGCATTATGCATATTGGTGTGCCCAGATCTAA

mRNA sequence

ATGGTCAACTTAATGAGCCATCATGTACAAGTCAATTTAAGTTGCCCTGTATGTCATGACGAGACCGAAACCACTAATCATGCACTATTTCGATGCCCCAGAGCCCGGGAGATATGGATGATTCTAATGCCAACCATATCGATGGATCAGTGTGATAGAATGGAGATTAAAGATCGCTGGTTAAGTTTTGCAGACTTTGCAAACCAGATTTTAGAATGCATTAGTGTGGGTGCATGGGCAATTTGGAATGACAGGAATAACCTAATCCGGGACTTACCAATTCCAAATTCTTCGGTTAAAAGTGAGTGGATTCAGGAATATTTATCAGAGTATTGGGCGGCCAATTCGATGGGAAGAGCGGTGGTACAGTCGAATGAAGATGTTAACACCATTATTTCGACAGGTGGAGATTTTGTCATGAGTGTTGATGCGTCTTTTCAGGATGATACGTCCAAATGCGGCATCGGTATGGTTCTGCGCACTAAACAAGGCAATGTTCTGGTTGCCCAGAATTTTCATGTTTCAGGCTGTCAATTCCCTCTGAGTGCTGAAGCTGTCGCGGTACTGTCAGGGCTGCAATTAGCGAGGAACTTGAAGGTTCGGAGTTTAACAGTCCTCTCTGACTGTTTGGCTCTAATACAGTCTATAAATGGTCGTATACAAGGACAATCAACAAGACCTCAAGGTCAATTTCCCGATGATCCCATGGACTGTACAAAGTCGGACATGATGGAGGCTGAATTTGCGTCGAACAGAGCCGTCGAAGGCGATTCTGTCCGCGAATTACTCACATTGGCTCGTCAATTCATCAATCAACGAAAGCCTTCCCAAGCCCTCCAAGCGGTTGTAATGGCAATGAGAACCCAAGGTGGGGATGCAGCAGTCTTTCAATCTTTGCACCGTGCCCGTGAGCTGTACAGAACTCGATTACAGGAGACTGCTGATGTTGATCAACTAGCTTCTTTATTTGCAGAGTGTGCTATAGCTGAAGCCCAGCCTCTGAACCCTGAACCTGCAGGAAGTAACATATCTGGTTCAGCAGTTGTCGAGGCTCACGGTAATTCTATACTGGCAGAAACTGGTAGGATGCAAGTTGTTATGGACGCCTTCTCAGATGGGAGCAGTTTTATTTGTTTGCAATGTGGGGGTCTTGTTAGTAACCATCGCAAAGATGAGCATTATGCATATTGGTGTGCCCAGATCTAA

Coding sequence (CDS)

ATGGTCAACTTAATGAGCCATCATGTACAAGTCAATTTAAGTTGCCCTGTATGTCATGACGAGACCGAAACCACTAATCATGCACTATTTCGATGCCCCAGAGCCCGGGAGATATGGATGATTCTAATGCCAACCATATCGATGGATCAGTGTGATAGAATGGAGATTAAAGATCGCTGGTTAAGTTTTGCAGACTTTGCAAACCAGATTTTAGAATGCATTAGTGTGGGTGCATGGGCAATTTGGAATGACAGGAATAACCTAATCCGGGACTTACCAATTCCAAATTCTTCGGTTAAAAGTGAGTGGATTCAGGAATATTTATCAGAGTATTGGGCGGCCAATTCGATGGGAAGAGCGGTGGTACAGTCGAATGAAGATGTTAACACCATTATTTCGACAGGTGGAGATTTTGTCATGAGTGTTGATGCGTCTTTTCAGGATGATACGTCCAAATGCGGCATCGGTATGGTTCTGCGCACTAAACAAGGCAATGTTCTGGTTGCCCAGAATTTTCATGTTTCAGGCTGTCAATTCCCTCTGAGTGCTGAAGCTGTCGCGGTACTGTCAGGGCTGCAATTAGCGAGGAACTTGAAGGTTCGGAGTTTAACAGTCCTCTCTGACTGTTTGGCTCTAATACAGTCTATAAATGGTCGTATACAAGGACAATCAACAAGACCTCAAGGTCAATTTCCCGATGATCCCATGGACTGTACAAAGTCGGACATGATGGAGGCTGAATTTGCGTCGAACAGAGCCGTCGAAGGCGATTCTGTCCGCGAATTACTCACATTGGCTCGTCAATTCATCAATCAACGAAAGCCTTCCCAAGCCCTCCAAGCGGTTGTAATGGCAATGAGAACCCAAGGTGGGGATGCAGCAGTCTTTCAATCTTTGCACCGTGCCCGTGAGCTGTACAGAACTCGATTACAGGAGACTGCTGATGTTGATCAACTAGCTTCTTTATTTGCAGAGTGTGCTATAGCTGAAGCCCAGCCTCTGAACCCTGAACCTGCAGGAAGTAACATATCTGGTTCAGCAGTTGTCGAGGCTCACGGTAATTCTATACTGGCAGAAACTGGTAGGATGCAAGTTGTTATGGACGCCTTCTCAGATGGGAGCAGTTTTATTTGTTTGCAATGTGGGGGTCTTGTTAGTAACCATCGCAAAGATGAGCATTATGCATATTGGTGTGCCCAGATCTAA

Protein sequence

MVNLMSHHVQVNLSCPVCHDETETTNHALFRCPRAREIWMILMPTISMDQCDRMEIKDRWLSFADFANQILECISVGAWAIWNDRNNLIRDLPIPNSSVKSEWIQEYLSEYWAANSMGRAVVQSNEDVNTIISTGGDFVMSVDASFQDDTSKCGIGMVLRTKQGNVLVAQNFHVSGCQFPLSAEAVAVLSGLQLARNLKVRSLTVLSDCLALIQSINGRIQGQSTRPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMAMRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISGSAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI

Homology

[Close]

BLAST of Lag0036984 vs. NCBI nr
Match: XP_022982291.1 (uncharacterized protein LOC111481167 [Cucurbita maxima] >XP_022982292.1 uncharacterized protein LOC111481167 [Cucurbita maxima])

HSP 1 Score: 315.1 bits (806), Expect = 8.8e-82
Identity = 157/176 (89.20%), Postives = 167/176 (94.89%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R QG+FPDDPMDC +SD+MEAEF++N AV GDSVRELLTLARQFINQRKPSQALQAVVMA
Sbjct: 5   RSQGKFPDDPMDCKQSDLMEAEFSANGAVAGDSVRELLTLARQFINQRKPSQALQAVVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. NCBI nr
Match: KAG7037793.1 (hypothetical protein SDJN02_01424, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.5 bits (802), Expect = 2.6e-81
Identity = 156/176 (88.64%), Postives = 167/176 (94.89%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R +G+FPDDPMDC +SD+MEAEF++N AV GDSVRELLTLARQFINQRKPSQALQAVVMA
Sbjct: 5   RSRGKFPDDPMDCEQSDLMEAEFSANGAVAGDSVRELLTLARQFINQRKPSQALQAVVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. NCBI nr
Match: KAG6608456.1 (hypothetical protein SDJN03_01798, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 311.6 bits (797), Expect = 9.8e-81
Identity = 155/176 (88.07%), Postives = 167/176 (94.89%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R +G+FPDDPMDC +SD+MEAEF++N AV GDSVRELLTLARQFINQRKPSQALQAVVMA
Sbjct: 5   RSRGKFPDDPMDCEQSDLMEAEFSANGAVAGDSVRELLTLARQFINQRKPSQALQAVVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVS+HRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSSHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. NCBI nr
Match: XP_023523427.1 (uncharacterized protein LOC111787641 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-80
Identity = 155/176 (88.07%), Postives = 166/176 (94.32%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R +G+FPDDPMDC +SD+MEAEF++N AV GDSVRELLTLARQ INQRKPSQALQAVVMA
Sbjct: 5   RSRGKFPDDPMDCKQSDLMEAEFSANGAVAGDSVRELLTLARQLINQRKPSQALQAVVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. NCBI nr
Match: KAG6591394.1 (hypothetical protein SDJN03_13740, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.0 bits (785), Expect = 2.4e-79
Identity = 156/176 (88.64%), Postives = 164/176 (93.18%), Query Frame = 0


Query: 228 QGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMAMR 287
           Q +F DD MDC+KSDMME EFASNRA EGDSVR+LLTLARQFI+QR P+QALQAVVMAMR
Sbjct: 366 QAKFADDTMDCSKSDMMETEFASNRAGEGDSVRDLLTLARQFIDQRNPTQALQAVVMAMR 425

Query: 288 TQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSN--ISG 347
           TQGGDAAVFQSLHRARELYRTR QETADVDQLASLFAECAIAEAQPLNPEP  SN  ISG
Sbjct: 426 TQGGDAAVFQSLHRARELYRTRFQETADVDQLASLFAECAIAEAQPLNPEPTDSNNDISG 485

Query: 348 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S VV+AHGNSILAETGRMQ+VMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCA+I
Sbjct: 486 SLVVDAHGNSILAETGRMQIVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCARI 541

[Close]

BLAST of Lag0036984 vs. ExPASy TrEMBL
Match: A0A6J1IYX7 (uncharacterized protein LOC111481167 OS=Cucurbita maxima OX=3661 GN=LOC111481167 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 4.3e-82
Identity = 157/176 (89.20%), Postives = 167/176 (94.89%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R QG+FPDDPMDC +SD+MEAEF++N AV GDSVRELLTLARQFINQRKPSQALQAVVMA
Sbjct: 5   RSQGKFPDDPMDCKQSDLMEAEFSANGAVAGDSVRELLTLARQFINQRKPSQALQAVVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. ExPASy TrEMBL
Match: A0A6J1CPE8 (uncharacterized protein LOC111012957 OS=Momordica charantia OX=3673 GN=LOC111012957 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.2e-79
Identity = 154/176 (87.50%), Postives = 166/176 (94.32%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           RPQG+  DD +D +KSDMMEAEFAS+RA +GD+VRELLTLARQFINQR PSQALQAVV A
Sbjct: 5   RPQGKVQDDAVDSSKSDMMEAEFASSRAGDGDAVRELLTLARQFINQRNPSQALQAVVTA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRARELYRTRLQE+ADVDQLASLFAECAIAEAQPLNPEP  SNISG
Sbjct: 65  MRTQGGDAAVFQSLHRARELYRTRLQESADVDQLASLFAECAIAEAQPLNPEPPESNISG 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
            +V++AHGNSILAETGRMQ+VMDAFSDGSSFICL+CGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 PSVMDAHGNSILAETGRMQIVMDAFSDGSSFICLRCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. ExPASy TrEMBL
Match: A0A6J1FSD6 (uncharacterized protein LOC111446812 OS=Cucurbita moschata OX=3662 GN=LOC111446812 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 3.4e-79
Identity = 153/176 (86.93%), Postives = 164/176 (93.18%), Query Frame = 0


Query: 226 RPQGQFPDDPMDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMA 285
           R + +FPDD MDC +SD+MEAEF++N AV GDSVRELLTLARQFINQRKPSQALQA VMA
Sbjct: 5   RSRRKFPDDSMDCKQSDLMEAEFSANGAVAGDSVRELLTLARQFINQRKPSQALQAFVMA 64

Query: 286 MRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSNISG 345
           MRTQGGDAAVFQSLHRA ELYRTRL+ETADVDQLAS+FAECAIAEAQPLNPEP   N+S 
Sbjct: 65  MRTQGGDAAVFQSLHRAGELYRTRLRETADVDQLASIFAECAIAEAQPLNPEPGECNMSS 124

Query: 346 SAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           S+VV+AHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI
Sbjct: 125 SSVVDAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 180

[Close]

BLAST of Lag0036984 vs. ExPASy TrEMBL
Match: A0A6J1FAR2 (uncharacterized protein LOC111443615 OS=Cucurbita moschata OX=3662 GN=LOC111443615 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 4.1e-77
Identity = 151/168 (89.88%), Postives = 159/168 (94.64%), Query Frame = 0


Query: 236 MDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMAMRTQGGDAAV 295
           MDC+KSDMME EFASNRA EGDSVR+LLTLARQFI+QR P+QALQAVVMAMRTQGGDAAV
Sbjct: 1   MDCSKSDMMETEFASNRAGEGDSVRDLLTLARQFIDQRNPTQALQAVVMAMRTQGGDAAV 60

Query: 296 FQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSN--ISGSAVVEAHG 355
           FQSLHRARE+YRTR QETADVDQLASLFAECAIAEAQPLNPEP  SN  ISGS VV+AHG
Sbjct: 61  FQSLHRAREMYRTRFQETADVDQLASLFAECAIAEAQPLNPEPTDSNNDISGSLVVDAHG 120

Query: 356 NSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           NSILAETGRMQ+VMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCA+I
Sbjct: 121 NSILAETGRMQIVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCARI 168

[Close]

BLAST of Lag0036984 vs. ExPASy TrEMBL
Match: A0A6J1ILE4 (uncharacterized protein LOC111477288 OS=Cucurbita maxima OX=3661 GN=LOC111477288 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 151/168 (89.88%), Postives = 158/168 (94.05%), Query Frame = 0


Query: 236 MDCTKSDMMEAEFASNRAVEGDSVRELLTLARQFINQRKPSQALQAVVMAMRTQGGDAAV 295
           MDC+KSDMME EFASNRA EGDSVR+LLTLARQFI+QR P+QALQAVVMAMRTQGGDAAV
Sbjct: 1   MDCSKSDMMETEFASNRAGEGDSVRDLLTLARQFIDQRNPTQALQAVVMAMRTQGGDAAV 60

Query: 296 FQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLNPEPAGSN--ISGSAVVEAHG 355
            QSLHRARELYRTR QETADVDQLASLFAECAIAEAQPLNPEP  SN  ISGS VV+AHG
Sbjct: 61  IQSLHRARELYRTRFQETADVDQLASLFAECAIAEAQPLNPEPTDSNNDISGSLVVDAHG 120

Query: 356 NSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
           NSILAETGRMQ+VMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCA+I
Sbjct: 121 NSILAETGRMQIVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCARI 168

[Close]

BLAST of Lag0036984 vs. TAIR 10
Match: AT5G47830.1 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 182.6 bits (462), Expect = 6.4e-46
Identity = 96/168 (57.14%), Postives = 124/168 (73.81%), Query Frame = 0


Query: 240 KSDMMEAEFASNRAVEGDS-VRELLTLARQFINQRKPSQALQAVVMAMRTQGGDAAVFQS 299
           ++ MM+A+      ++  S  R+ L+ AR+ ++Q +PSQALQAVVMAMR QGGD AV Q 
Sbjct: 6   ETQMMDADSTETFMLDKPSTARDFLSAARRLVDQGEPSQALQAVVMAMRNQGGDEAVLQI 65

Query: 300 LHRARELYRTRLQETADVDQLASLFAECAIAEAQPLN-PEPAGS----NISGSAVVEAHG 359
           L+R RELY+ R+QETA +DQLAS+FAECAI EAQPL   EP  S        +   +AHG
Sbjct: 66  LNRTRELYKRRIQETASMDQLASIFAECAITEAQPLALDEPTTSKDLFGTKQTVTADAHG 125

Query: 360 NSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWCAQI 402
            SIL ++GR Q+++DAF+DGSSFICLQCGGLVS HR+DEHYAYWC+ +
Sbjct: 126 ISILEKSGRSQIMLDAFADGSSFICLQCGGLVSIHRRDEHYAYWCSNM 173

[Close]

BLAST of Lag0036984 vs. TAIR 10
Match: AT5G47830.2 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 154.8 bits (390), Expect = 1.4e-37
Identity = 78/123 (63.41%), Postives = 94/123 (76.42%), Query Frame = 0


Query: 284 MAMRTQGGDAAVFQSLHRARELYRTRLQETADVDQLASLFAECAIAEAQPLN-PEPAGS- 343
           MAMR QGGD AV Q L+R RELY+ R+QETA +DQLAS+FAECAI EAQPL   EP  S 
Sbjct: 1   MAMRNQGGDEAVLQILNRTRELYKRRIQETASMDQLASIFAECAITEAQPLALDEPTTSK 60

Query: 344 ---NISGSAVVEAHGNSILAETGRMQVVMDAFSDGSSFICLQCGGLVSNHRKDEHYAYWC 402
                  +   +AHG SIL ++GR Q+++DAF+DGSSFICLQCGGLVS HR+DEHYAYWC
Sbjct: 61  DLFGTKQTVTADAHGISILEKSGRSQIMLDAFADGSSFICLQCGGLVSIHRRDEHYAYWC 120

[Close]

BLAST of Lag0036984 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 9.6e-10
Identity = 55/229 (24.02%), Postives = 98/229 (42.79%), Query Frame = 0


Query: 4   LMSHHVQVNLSCPVCHDETETTNHALFRCPRAREIWMILMPTISMDQCDRMEIKDRWLSF 63
           L +  ++++ SCP CH E E+ NHALF CP A   W +   ++  +Q    + ++   + 
Sbjct: 189 LTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNI 248

Query: 64  ADFA-----NQILECISVG-AWAIWNDRNNLI--RDLPIPNSSVKS------EWIQEYLS 123
            +F      +   + + V   W IW  RNN++  +    P+ +V S      +W+    S
Sbjct: 249 LNFVQDTTMSDFHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQS 308

Query: 124 EYWAANSMGRAVVQSNEDVNTIISTGGDFVMSVDASFQDDTSKCGIGMVLRTKQGNVLVA 183
            +    S  R + ++  +     +T      + DA F     +   G ++R   G  +  
Sbjct: 309 -HKKTPSPTRQIAENKIEWRNPPAT--YVKCNFDAGFDVQKLEATGGWIIRNHYGTPISW 368

Query: 184 QNFHVSGCQFPLSAEAVAVLSGLQLARNLKVRSLTVLSDCLALIQSING 219
            +  ++    PL AE  A+L+ LQ         + +  DC  LI  ING
Sbjct: 369 GSMKLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING 414

[Close]

BLAST of Lag0036984 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 4.5e-07
Identity = 52/220 (23.64%), Postives = 87/220 (39.55%), Query Frame = 0


Query: 8   HVQVNLSCPVCHDETETTNHALFRCPRAREIWMIL-MPTISMDQ-CDRMEIKDRWLSFAD 67
           H+    SC  C D  ET NH LF+C  AR +W I  +P     +  D +     W+   +
Sbjct: 5   HLFREASCVRCPDSRETVNHLLFKCCFARLVWAISPIPAYPEGEWTDSLYANLYWVLNLE 64

Query: 68  F----ANQILECISVGAWAIWNDRNNLI---RDLPIPNSSVKS-EWIQEYLSEYWAANSM 127
                  +I   +    W +W  RN L+   ++   P    ++ E  +E+ +        
Sbjct: 65  VEIPKLGKIGNLVPWLLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKA 124

Query: 128 GRAVVQSNEDVNTIISTGGDFVMSVDASFQDDTSKCGIGMVLRTKQGNVLVAQNFHVSGC 187
               V+ N  V            + DA++Q +  +CGIG +LR + G VL      +   
Sbjct: 125 SGPQVERNLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRT 184

Query: 188 QFPLSAEAVAVLSGLQLARNLKVRSLTVLSDCLALIQSIN 218
           +  L AE  A+   +        + +   SD  AL+  +N
Sbjct: 185 KNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLN 224

The following BLAST results are available for this feature:

BLAST of Lag0036984 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982291.1	8.8e-82	89.20	uncharacterized protein LOC111481167 [Cucurbita maxima] >XP_022982292.1 uncharac...	[more]
KAG7037793.1	2.6e-81	88.64	hypothetical protein SDJN02_01424, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6608456.1	9.8e-81	88.07	hypothetical protein SDJN03_01798, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023523427.1	1.3e-80	88.07	uncharacterized protein LOC111787641 [Cucurbita pepo subsp. pepo]	[more]
KAG6591394.1	2.4e-79	88.64	hypothetical protein SDJN03_13740, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lag0036984 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0036984 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYX7	4.3e-82	89.20	uncharacterized protein LOC111481167 OS=Cucurbita maxima OX=3661 GN=LOC111481167...	[more]
A0A6J1CPE8	1.2e-79	87.50	uncharacterized protein LOC111012957 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1FSD6	3.4e-79	86.93	uncharacterized protein LOC111446812 OS=Cucurbita moschata OX=3662 GN=LOC1114468...	[more]
A0A6J1FAR2	4.1e-77	89.88	uncharacterized protein LOC111443615 OS=Cucurbita moschata OX=3662 GN=LOC1114436...	[more]
A0A6J1ILE4	1.2e-76	89.88	uncharacterized protein LOC111477288 OS=Cucurbita maxima OX=3661 GN=LOC111477288...	[more]

BLAST of Lag0036984 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47830.1	6.4e-46	57.14	unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae ...	[more]
AT5G47830.2	1.4e-37	63.41	unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae ...	[more]
AT3G09510.1	9.6e-10	24.02	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	4.5e-07	23.64	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 3..39 e-value: 9.1E-7 score: 29.5
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 142..224 e-value: 8.2E-15 score: 54.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 137..277 e-value: 1.0E-9 score: 40.4
None	No IPR available	PANTHER	PTHR35513	OS02G0158600 PROTEIN	coord: 237..400
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 142..224 e-value: 4.15011E-12 score: 60.7908
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 137..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036984.1	Lag0036984.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity