Lag0036961 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036961
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	chr2: 2417168 .. 2417989 (-)
RNA-Seq Expression	Lag0036961
Synteny	Lag0036961

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATCTTGTAATTCATGTATTCAACCATTCTTTGAATCTCAACTATTCATGTATGTAACTAAATGTGATTCATGTCCCTTGAATTATGTCTAGATTAATTTTAACAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCGCCTTCCACCAAGAATCGGCTCTATTAGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGTATTGTTTTCGAATACAAACACTTTGGTGGATGTGTTTTCTTCAACACCCTCTACAAAGCCAAAGTAAGAACAAAACTCCCCTTTTAATTTTTAGCATCCACTTCTTTTTTCAAAGATTTAAAAAAAAAAAAGAAAGTAAGTTATAAAAACATTTTTATTAATATTTTTTTCTTGGTGTAGATTCTGAGGGAGCAACCAAATAAAGAACTATTTCTCATTGCTTTGGTTAATACCCAAGTATTTATTTGCATGGTGGGAGCTGATAAGGAGTTTCATAATCCCAATAGTGAAGGGTTCAGTTTACTATGAGGGTTTGCTAATGAAAGGGGTGAGGGTGTTTGGATTGATTATTCCAGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTTTTACAGATACCCGATGTGATCGAAGACCCAATTCTTCGAAACTACGTCGAAGCCGACAGCTAA

mRNA sequence

ATGTATCTTATTAATTTTAACAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCGCCTTCCACCAAGAATCGGCTCTATTAGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTTTTACAGATACCCGATGTGATCGAAGACCCAATTCTTCGAAACTACGTCGAAGCCGACAGCTAA

Coding sequence (CDS)

ATGTATCTTATTAATTTTAACAGGTTCATCATCACTGGCCACAGCCTGGGCGGAGCCCTCGCAACCCTCTTTGTCACTCTGCTCGCCTTCCACCAAGAATCGGCTCTATTAGAGAAGTTGCAGGCTGTTTACACCTATGGCCAGCCCAGAGTCGGAGACCAGCAATTTGCAGAGTTCATGGTAAATACTATCCACAAATATGGCTTCAAATACTACAGATACGTTTACTCTAATGACTTGGTGCCTAGGATTCCTGCTGATGGAGCCGCCGCCCACTTTCCTCAGGACTATGTTAATTCGACCCGTTTGGGACTTTTACAGATACCCGATGTGATCGAAGACCCAATTCTTCGAAACTACGTCGAAGCCGACAGCTAA

Protein sequence

MYLINFNRFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGAAAHFPQDYVNSTRLGLLQIPDVIEDPILRNYVEADS

Homology

[Close]

BLAST of Lag0036961 vs. NCBI nr
Match: KAG6608469.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 160.2 bits (404), Expect = 1.1e-35
Identity = 90/197 (45.69%), Postives = 105/197 (53.30%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RFIITGHSLGGALATLFVTLL+ H E+ +LEKLQ VYTYGQPRVGD++FAEFMVNT+ +Y
Sbjct: 261 RFIITGHSLGGALATLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQQY 320

Query: 68  GFKYYRYVYSNDLVPRIPADGA-------------------------------------- 125
           GFKYYRYVYS+DLVPRIP+DG                                       
Sbjct: 321 GFKYYRYVYSSDLVPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIP 380

[Close]

BLAST of Lag0036961 vs. NCBI nr
Match: XP_022982229.1 (uncharacterized protein LOC111481121 [Cucurbita maxima])

HSP 1 Score: 158.3 bits (399), Expect = 4.3e-35
Identity = 90/197 (45.69%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RFIITGHSLGGALATLFVTLL+ H E+ +LEKLQ VYTYGQPRVGD++FAEFMVNT+ KY
Sbjct: 286 RFIITGHSLGGALATLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKY 345

Query: 68  GFKYYRYVYSNDLVPRIPADGA-------------------------------------- 125
           GFKYYRYVYS+DLVPRIP+DG                                       
Sbjct: 346 GFKYYRYVYSSDLVPRIPSDGVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIP 405

[Close]

BLAST of Lag0036961 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 156.8 bits (395), Expect = 1.3e-34
Identity = 89/197 (45.18%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RFIITGHSLGGALATLFVTLL+ H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +Y
Sbjct: 286 RFIITGHSLGGALATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQY 345

Query: 68  GFKYYRYVYSNDLVPRIPADGA-------------------------------------- 125
           GFKYYRYVYS+DLVPRIP+DG                                       
Sbjct: 346 GFKYYRYVYSSDLVPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIP 405

[Close]

BLAST of Lag0036961 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 156.8 bits (395), Expect = 1.3e-34
Identity = 88/197 (44.67%), Postives = 104/197 (52.79%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RFIITGHSLGGALATLFVT+L+ H ES +LEKL+ +YTYGQPRVGDQQFA+FMVNT+ KY
Sbjct: 286 RFIITGHSLGGALATLFVTVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKY 345

Query: 68  GFKYYRYVYSNDLVPRIPAD---------------------------------------- 125
           GFKY+RYVYS+DLVPR+P+D                                        
Sbjct: 346 GFKYHRYVYSSDLVPRLPSDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIP 405

[Close]

BLAST of Lag0036961 vs. NCBI nr
Match: XP_023523371.1 (uncharacterized protein LOC111787588 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-33
Identity = 89/197 (45.18%), Postives = 103/197 (52.28%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RFIITGHSLGGALATLFVTLL+ H E+A+LEKLQ VYTYGQPRVGD++FAEFMVN++ KY
Sbjct: 286 RFIITGHSLGGALATLFVTLLSMHDETAVLEKLQGVYTYGQPRVGDRRFAEFMVNSVQKY 345

Query: 68  GFKYYRYVYSNDLVPRIPADGA-------------------------------------- 125
           GFKYYRYVYS+DLVPRIP+DG                                       
Sbjct: 346 GFKYYRYVYSSDLVPRIPSDGVIFKYKHFARSIYFNSLYKGRIVKEQPNKNYFSLLWVIP 405

[Close]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.9e-18
Identity = 43/80 (53.75%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           +F++TGHSLGGALA LF  +L  H+E  ++E+L  +YTYGQPRVG++Q   FM   +   
Sbjct: 386 KFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEHP 445

Query: 68  GFKYYRYVYSNDLVPRIPAD 88
             KY+R VY NDLVPR+P D
Sbjct: 446 VPKYFRVVYCNDLVPRLPYD 465

[Close]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-17
Identity = 40/80 (50.00%), Postives = 61/80 (76.25%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           RF++TGHSLGGALA LF TLL  ++E+ ++++L  VYT+GQPR+G+++   FM   +++ 
Sbjct: 332 RFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQP 391

Query: 68  GFKYYRYVYSNDLVPRIPAD 88
             +Y+R VY ND+VPR+P D
Sbjct: 392 VDRYFRVVYCNDIVPRLPYD 411

[Close]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.2e-17
Identity = 40/80 (50.00%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           +F++TGHSLGGALA LF  +L   QE+ +L++L  VYT+GQPR+G+     FM N ++  
Sbjct: 333 KFVVTGHSLGGALAILFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFP 392

Query: 68  GFKYYRYVYSNDLVPRIPAD 88
             +Y+R VY ND+VPR+P D
Sbjct: 393 ERRYFRVVYCNDMVPRVPFD 412

[Close]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 3.6e-08
Identity = 34/75 (45.33%), Postives = 43/75 (57.33%), Query Frame = 0


Query: 11  ITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFK 70
           +TGHSLGGA A L   L  + +E  L      +YT GQPRVGD  FA ++V+T    G  
Sbjct: 234 VTGHSLGGATA-LLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVST----GIP 293

Query: 71  YYRYVYSNDLVPRIP 86
           Y R V   D+VP +P
Sbjct: 294 YRRTVNERDIVPHLP 303

[Close]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.8e-07
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 8   RFIITGHSLGGALATLFVTLLAFHQESALLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKY 67
           R + TGHSLGGALAT+    L  +           V++YG PRVG++ FAEF+     + 
Sbjct: 161 RVVFTGHSLGGALATVAGADLRGNGYDI------DVFSYGAPRVGNRAFAEFLT---VQT 220

Query: 68  GFKYYRYVYSNDLVPRIPAD--GAAAHFPQDYVNSTRLGLLQIPDVIE 114
           G   YR  ++ND+VPR+P    G +   P+ ++ S  L  +   D+++
Sbjct: 221 GGTLYRITHTNDIVPRLPPREFGYSHSSPEYWIKSGTLVPVTRNDIVK 259

The following BLAST results are available for this feature:

BLAST of Lag0036961 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6608469.1	1.1e-35	45.69	Lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982229.1	4.3e-35	45.69	uncharacterized protein LOC111481121 [Cucurbita maxima]	[more]
XP_022940183.1	1.3e-34	45.18	uncharacterized protein LOC111445886 [Cucurbita moschata]	[more]
XP_038899591.1	1.3e-34	44.67	triacylglycerol lipase OBL1-like [Benincasa hispida]	[more]
XP_023523371.1	1.8e-33	45.18	uncharacterized protein LOC111787588 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0036961 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	2.9e-18	53.75	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	1.4e-17	50.00	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	4.2e-17	50.00	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
P19515	3.6e-08	45.33	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]
O59952	1.8e-07	35.19	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 6..119 e-value: 1.4E-28 score: 102.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 8..89
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 8..87 e-value: 5.4E-25 score: 87.9
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 8..88
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 8..88
None	No IPR available	CDD	cd00519	Lipase_3	coord: 8..94 e-value: 1.19971E-21 score: 84.065

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036961.1	Lag0036961.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity