Lag0036952 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036952
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cold regulated protein 27
Location	chr2: 2353165 .. 2355852 (-)
RNA-Seq Expression	Lag0036952
Synteny	Lag0036952

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTTTGCCTCGAAATTTGGAGAATCTGAAGGCCAGAAAAACTCGGACCGACTCACTGATCTCTGAATTGGCCAAGGACGAGTCAACCGAGTTTGCCCTCCCGGAACAATCTTCTAGCCAGGTACTTACCCTACTTTTCTTCCTCATAGTTATAATTATCTTCGTTTTAGATGTTTTTGTTGTTCCGATTTCGTTTTGGTTCTGTGATGTTTCGTATCTTCAATTCCATGCTCTGTGACCCAACTGTCTCTCTAATTTCGCGCTAAAAGTGAAAAGATGTCAATTAAATATTAGAAAGAAAGAGGAAGTGGAAGAAGACGGAACTGGTCTCATACTTGACTCTTGAGACCGGGGGATTTTATTTTGTATTTCGTTCGCCTCTGTTTGGATCGATTTTGCCCTCTGTCGATGGAATTATTGTAGAGCTGTGTAAGGGGTTTGGATCTGTGACCAATTATTCTGGAAATTCAAAACGAGTTTTGATTGTTCGATTTTATGATTTGTCATTATGATGACTAATGGTAGCGTTTTTCTGTTATTTATTAAGAGAGACCATGAACTTGCCGCGGGAATTGCTTATAATGCGGTTTCGGTTTTAGGGCACTCATATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATCTGTTCCTTGAATCCATGGAGGCATCATTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGTTGGTTCCGGCTCCGGCAAGGAGAATTCTTCACGCACAAAATTGCATGGCCAAAGCCAGTCCGCTTCTCATGTCCATTCACATTTTTCCCAGGTTCAGTGCTGATTGCTCTTTGTTGACGAGTTTTAACTCTTTTCTAATCATGCCTTGTTAATGCTTTTTTTGTTCTTTTTGTTGCTTTTATCTACACAGTTTAAGGTTCTTCGACGAGGCAGCTGGAAGAACATTAATTTTGAACCAACTGAATCTCGTCCGAATTTATTGAATGATTACCAAGCTCTCTCGCGTAATCCATGGATACATCACTTCAGAGCTGCTCGCAAAAACAAAAATGTTGCATGCAGAGGTCAAGCTATCAGTTCAAGAGGAAAGAATTTTTTGCCATTCGGAGCAGCTAATAATTCAGGACCTATGCGTGCTTGTAACTCTAATTTGTCTCAAGAATATATCAACAATAAAGGTAGATCTTCGGGCCTTTGACGTTATAAATTATCAATATTTGTGCCTCTCCTTGGTTGTAATTTAGTTTATCATGTGTAAGTTTGTTAGTGATTCTAATTTGGAAGATGTTTGCTCTGTATCTAGTCTTTTTTACCAGAAAGTTCTGAGTTAAAACAAATGATGTGACTGAAGTGAAGCTGATTTTTCTTCTAATCTACTTTGTCCAAGCTTAATTGTAATCTGTATAGCAATCATCATTTCGCTGATGTGCAACTAAATTTTAGATGGGAAAGACTTTTAATTTAATTTTAAGCTCTACAAATGATTTTATGTTGCAGAAGCGTCAGATCAGAACTTTGTCGATGAAGATAGAGAAGGTGAAAAAGGAAGCAGTGATTGCAATGCCAAAAGGGTCAAAATTCCAGAAAACAATGGCTTAATCAATGACCAGGTAATTCAAATGACATCAACTTAGGTCCCCTTTGATAACCATTCACCTTTTAGTTTTTTGTTTTTGAAAAATTAAGTTTATAAACACTACTCTCACCTATGAAATTCTTTGTTTTGCTATTTACTTTCAAAACGTGCTTTTAAAATCCAACCCAAGCTTTAAAAACAAAGGGTAGTTGTTGGTTTTTGGAACTTGACTAAGAATTCAAATGTTTCTTTAAGAAAAATGAGAACTATAGTAAAAAACGAAATCATTATCAAATCGTGCCTTGGCTATCATTCTTGCTACAAAGCGATAACAGCTGATTTCTTTTAGGTGGTTCCTTAAGTTCCATGAAAAAGAAGATGATGTTTAACAAGAATTCCATTACAGCCAGAAGATAGCTAGATCCAAGATTTTCGCCAAGACGAGACAAAAGACTTATCGACTACTTTTCTAACTCAGGAAAGGCTATAGATGATCAGAGATTGTTGGTGAAGTCTATAATTCTCATTTTGATGTTCTTGGTTTGTCTGTTAATTTAAGTTCTAATACCCTTGGGTGTCTGCAGTTGTGTAGTAATTAGTAAATCAGAAATTATGGTTTTGACTGGGTAGGTTTGTAAGGTAATGTCATTCACATTCAGCATATGTATATATACATATATATTTCTTTTGGTACAACAAGATTGTGCAACATTTGTTTCAAGTTTTTTCTCCTCTCTCGTTTCAAGATAAACAGAAGAGCGCTAAATGAAATAGTAAGAAATTTTCAATTTTCTCTCCAATCATTCAGGGAGTCGTTTAGTTGAACTAGATTTTTATGCCATTGATTTTTTTGTCAATTGAGATAGATTTTGAGATAGATTTTCATTCATGTCGTTTCGTTTCTTTTGCCTTGGTCCATTAAATAAAGATAACAAGATTTTCGCTATTGCCTTCGATTGGTCATTTTTCTTTCACAGGTTTGGATCTTTTTGAAGTTTCTTTCCATCTTTGTGGTTGACAAGCACCGTAGGATGGGCATAAGTGAAAATGTTTTGGGAAAATACAAAATAATATACACCGGACAAAAGCCTGATGGATATGAGTCAATTTAG

mRNA sequence

ATGGAGTTTTTGCCTCGAAATTTGGAGAATCTGAAGGCCAGAAAAACTCGGACCGACTCACTGATCTCTGAATTGGCCAAGGACGAGTCAACCGAGTTTGCCCTCCCGGAACAATCTTCTAGCCAGGGCACTCATATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATCTGTTCCTTGAATCCATGGAGGCATCATTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGTTGGTTCCGGCTCCGGCAAGGAGAATTCTTCACGCACAAAATTGCATGGCCAAAGCCAGTCCGCTTCTCATGTCCATTCACATTTTTCCCAGTTTAAGGTTCTTCGACGAGGCAGCTGGAAGAACATTAATTTTGAACCAACTGAATCTCGTCCGAATTTATTGAATGATTACCAAGCTCTCTCGCGTAATCCATGGATACATCACTTCAGAGCTGCTCGCAAAAACAAAAATGTTGCATGCAGAGGTCAAGCTATCAGTTCAAGAGGAAAGAATTTTTTGCCATTCGGAGCAGCTAATAATTCAGGACCTATGCGTGCTTGTAACTCTAATTTGTCTCAAGAATATATCAACAATAAAGAAGCGTCAGATCAGAACTTTGTCGATGAAGATAGAGAAGGTGAAAAAGGAAGCAGTGATTGCAATGCCAAAAGGGTCAAAATTCCAGAAAACAATGGCTTAATCAATGACCAGGTTTGGATCTTTTTGAAGTTTCTTTCCATCTTTGTGGTTGACAAGCACCGTAGGATGGGCATAAGTGAAAATGTTTTGGGAAAATACAAAATAATATACACCGGACAAAAGCCTGATGGATATGAGTCAATTTAG

Coding sequence (CDS)

ATGGAGTTTTTGCCTCGAAATTTGGAGAATCTGAAGGCCAGAAAAACTCGGACCGACTCACTGATCTCTGAATTGGCCAAGGACGAGTCAACCGAGTTTGCCCTCCCGGAACAATCTTCTAGCCAGGGCACTCATATGACGGAGTCTGTACCTTCTGATTGGACCGATGAGAAGCACCATCTGTTCCTTGAATCCATGGAGGCATCATTTGTCAGCCAGATGTTTGATTCTGCGCATTCAGTTGGTTCCGGCTCCGGCAAGGAGAATTCTTCACGCACAAAATTGCATGGCCAAAGCCAGTCCGCTTCTCATGTCCATTCACATTTTTCCCAGTTTAAGGTTCTTCGACGAGGCAGCTGGAAGAACATTAATTTTGAACCAACTGAATCTCGTCCGAATTTATTGAATGATTACCAAGCTCTCTCGCGTAATCCATGGATACATCACTTCAGAGCTGCTCGCAAAAACAAAAATGTTGCATGCAGAGGTCAAGCTATCAGTTCAAGAGGAAAGAATTTTTTGCCATTCGGAGCAGCTAATAATTCAGGACCTATGCGTGCTTGTAACTCTAATTTGTCTCAAGAATATATCAACAATAAAGAAGCGTCAGATCAGAACTTTGTCGATGAAGATAGAGAAGGTGAAAAAGGAAGCAGTGATTGCAATGCCAAAAGGGTCAAAATTCCAGAAAACAATGGCTTAATCAATGACCAGGTTTGGATCTTTTTGAAGTTTCTTTCCATCTTTGTGGTTGACAAGCACCGTAGGATGGGCATAAGTGAAAATGTTTTGGGAAAATACAAAATAATATACACCGGACAAAAGCCTGATGGATATGAGTCAATTTAG

Protein sequence

MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSWKNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAANNSGPMRACNSNLSQEYINNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQVWIFLKFLSIFVVDKHRRMGISENVLGKYKIIYTGQKPDGYESI

Homology

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BLAST of Lag0036952 vs. NCBI nr
Match: XP_038896198.1 (cold-regulated protein 27 isoform X1 [Benincasa hispida])

HSP 1 Score: 384.4 bits (986), Expect = 8.3e-103
Identity = 203/246 (82.52%), Postives = 217/246 (88.21%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LPRNLENLKA +TR+DS++SE AKDESTEFALP  SSSQGTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPRNLENLKAVETRSDSIVSEFAKDESTEFALPVVSSSQGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDS HSVGS   KENSSR KL GQSQSASHVHSHF QFKVLRRGSW
Sbjct: 61  LFLESMEASFVSQMFDSVHSVGSCPSKENSSRAKLLGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFEPTESR NLLNDYQALS NPWIHHFRAARKNKNVAC+ Q+I SRG+N LPFGAAN
Sbjct: 121 KNINFEPTESRSNLLNDYQALSHNPWIHHFRAARKNKNVACKSQSIGSRGRNSLPFGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
           NSG MRA +S+LSQ++I +NKE SDQNFVDE  E EKGSSDCNAKRVK P+ N LINDQV
Sbjct: 181 NSGSMRASDSDLSQQFISSNKEVSDQNFVDE--EAEKGSSDCNAKRVKTPQTNALINDQV 240

Query: 241 WIFLKF 246
             FLKF
Sbjct: 241 --FLKF 242

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BLAST of Lag0036952 vs. NCBI nr
Match: XP_038896200.1 (cold-regulated protein 27 isoform X3 [Benincasa hispida])

HSP 1 Score: 380.2 bits (975), Expect = 1.6e-101
Identity = 198/239 (82.85%), Postives = 212/239 (88.70%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LPRNLENLKA +TR+DS++SE AKDESTEFALP  SSSQGTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPRNLENLKAVETRSDSIVSEFAKDESTEFALPVVSSSQGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDS HSVGS   KENSSR KL GQSQSASHVHSHF QFKVLRRGSW
Sbjct: 61  LFLESMEASFVSQMFDSVHSVGSCPSKENSSRAKLLGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFEPTESR NLLNDYQALS NPWIHHFRAARKNKNVAC+ Q+I SRG+N LPFGAAN
Sbjct: 121 KNINFEPTESRSNLLNDYQALSHNPWIHHFRAARKNKNVACKSQSIGSRGRNSLPFGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQ 239
           NSG MRA +S+LSQ++I +NKE SDQNFVDE  E EKGSSDCNAKRVK P+ N LINDQ
Sbjct: 181 NSGSMRASDSDLSQQFISSNKEVSDQNFVDE--EAEKGSSDCNAKRVKTPQTNALINDQ 237

[Close]

BLAST of Lag0036952 vs. NCBI nr
Match: XP_038896199.1 (cold-regulated protein 27 isoform X2 [Benincasa hispida])

HSP 1 Score: 380.2 bits (975), Expect = 1.6e-101
Identity = 198/239 (82.85%), Postives = 212/239 (88.70%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LPRNLENLKA +TR+DS++SE AKDESTEFALP  SSSQGTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPRNLENLKAVETRSDSIVSEFAKDESTEFALPVVSSSQGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDS HSVGS   KENSSR KL GQSQSASHVHSHF QFKVLRRGSW
Sbjct: 61  LFLESMEASFVSQMFDSVHSVGSCPSKENSSRAKLLGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFEPTESR NLLNDYQALS NPWIHHFRAARKNKNVAC+ Q+I SRG+N LPFGAAN
Sbjct: 121 KNINFEPTESRSNLLNDYQALSHNPWIHHFRAARKNKNVACKSQSIGSRGRNSLPFGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQ 239
           NSG MRA +S+LSQ++I +NKE SDQNFVDE  E EKGSSDCNAKRVK P+ N LINDQ
Sbjct: 181 NSGSMRASDSDLSQQFISSNKEVSDQNFVDE--EAEKGSSDCNAKRVKTPQTNALINDQ 237

[Close]

BLAST of Lag0036952 vs. NCBI nr
Match: KAG6608473.1 (Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.9 bits (969), Expect = 7.8e-101
Identity = 197/243 (81.07%), Postives = 212/243 (87.24%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLENLK ++TRTDSLISELA DESTE  L ++SSSQGT MTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDSAHS G+GSGKENSS TKLHGQSQ+ASHVHSH  QFKVLR+GSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KN+NFEPTE+RPNLLNDYQALSRNPWIHHFRAAR+NKNVAC+  AISSR +N LPFG  N
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARRNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGE---KGSSDCNAKRVKIPENNGLIN 240
           NSGPMRACNS+LSQ YI NNKE SDQNFVD +RE E   KGS+DCN KRVK  E N LIN
Sbjct: 233 NSGPMRACNSDLSQHYIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

[Close]

BLAST of Lag0036952 vs. NCBI nr
Match: XP_022982275.1 (uncharacterized protein LOC111481154 [Cucurbita maxima])

HSP 1 Score: 377.1 bits (967), Expect = 1.3e-100
Identity = 197/243 (81.07%), Postives = 212/243 (87.24%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLENLK ++TRTDSLISELA DESTE  L ++SSSQGT MTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDSAHS G+GSGKENSS TKLHGQSQ+ASHVHSH  QFKVLR+GSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KN+NFEPTE+RPNLLNDYQALSRNPWIHHFRAARKNKNVAC+  AISSR +N LPFG  N
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGE---KGSSDCNAKRVKIPENNGLIN 240
           NSGPMRACNS+LS+ YI NNKE SDQNFVD +RE E   KGS+DCN KRVK  E N LIN
Sbjct: 233 NSGPMRACNSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

[Close]

BLAST of Lag0036952 vs. ExPASy Swiss-Prot
Match: Q8L8T7 (Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 72/211 (34.12%), Postives = 109/211 (51.66%), Query Frame = 0


Query: 37  EQSSSQGTHMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLH 96
           E +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S  ++G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSLGALGK---NENVSESTRF 90

Query: 97  GQSQSASHVHSHFSQFKVLRRGSWKNINFEPTESRPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ IN +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RKNKNVAC---RGQAISSRG--KNFLPFGAANNSGPMRACNSNLSQEYINNKEASDQNFV 216
            K +         Q +SS    K     G+A++   + + + +  Q  +   E SDQNFV
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFV 210

Query: 217 DEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
           +E  +GE GSS    K V + E++    DQV
Sbjct: 211 NEGIKGENGSSK-KMKTVMMSESSS--TDQV 229

[Close]

BLAST of Lag0036952 vs. ExPASy Swiss-Prot
Match: Q8L983 (Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.7e-05
Identity = 59/229 (25.76%), Postives = 91/229 (39.74%), Query Frame = 0


Query: 19  DSLISELAKDESTEFALPEQSSSQGTHMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E  L     S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSWKNINFEPTES 138
           V Q++        G G +  SRT+        S+ H    QF VL+ G W+ +NF   +S
Sbjct: 75  VRQLYSLL-----GGGTQRLSRTR-----DVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAANNSGPMRACNS 198
                     L  +      R + +NK     G                N +        
Sbjct: 135 ---------CLETSSEFRFHRNSLRNKPENSNG----------------NYTMGTTVQGD 194

Query: 199 NLSQEYINNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
            L  +   + EAS QNF +E+ E EKG      +R    +++ L  DQV
Sbjct: 195 VLCHDETKHSEASGQNFREEEEEEEKGEVSKKREREANNDDSSLKEDQV 208

[Close]

BLAST of Lag0036952 vs. ExPASy TrEMBL
Match: A0A6J1J278 (uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 6.5e-101
Identity = 197/243 (81.07%), Postives = 212/243 (87.24%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLENLK ++TRTDSLISELA DESTE  L ++SSSQGT MTESVPSDWTDEKHH
Sbjct: 53  MELLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 112

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDSAHS G+GSGKENSS TKLHGQSQ+ASHVHSH  QFKVLR+GSW
Sbjct: 113 LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 172

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KN+NFEPTE+RPNLLNDYQALSRNPWIHHFRAARKNKNVAC+  AISSR +N LPFG  N
Sbjct: 173 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGGDN 232

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGE---KGSSDCNAKRVKIPENNGLIN 240
           NSGPMRACNS+LS+ YI NNKE SDQNFVD +RE E   KGS+DCN KRVK  E N LIN
Sbjct: 233 NSGPMRACNSDLSRHYIYNNKEMSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 292

[Close]

BLAST of Lag0036952 vs. ExPASy TrEMBL
Match: A0A6J1FJA4 (uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445977 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 8.7e-98
Identity = 195/243 (80.25%), Postives = 209/243 (86.01%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLENLK ++TRTDSLISELA DESTE  L ++SSSQGT MTESVPSDWTDEKHH
Sbjct: 1   MEVLPPNLENLKPKETRTDSLISELAMDESTEAPLQDESSSQGTRMTESVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESMEASFVSQMFDSAHS G+GSGKENSS TKLHGQSQ+ASHVHSH  QFKVLR+GSW
Sbjct: 61  LFLESMEASFVSQMFDSAHSGGTGSGKENSSWTKLHGQSQAASHVHSHVGQFKVLRQGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KN+NFEPTE+RPNLLNDYQALSRNPWIHHFRAARKNKNVAC+  AISSR +N LPFGA  
Sbjct: 121 KNVNFEPTEARPNLLNDYQALSRNPWIHHFRAARKNKNVACKSPAISSRKRNLLPFGA-- 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGE---KGSSDCNAKRVKIPENNGLIN 240
             GPMRACNS+LSQ  I NNKE SDQNFVD +RE E   KGS+DCN KRVK  E N LIN
Sbjct: 181 --GPMRACNSDLSQHCIYNNKEVSDQNFVDGEREVEKVAKGSNDCNTKRVKTSETNDLIN 239

[Close]

BLAST of Lag0036952 vs. ExPASy TrEMBL
Match: A0A6J1F6L8 (uncharacterized protein LOC111442637 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442637 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 2.1e-96
Identity = 191/240 (79.58%), Postives = 205/240 (85.42%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLEN  A +TRTDSLISELAKDESTEFALP +SSS GTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHNAVETRTDSLISELAKDESTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESME SFVSQMFDS HSVGS   KENSS TKLHGQSQSASHVHSHF QFKVLRRGSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFE TESR NLLND+QALSRNPWIHHFRA RKNKNVAC+ QAI S G+N  P GAAN
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDC-NAKRVKIPENNGLINDQ 239
           +S P+RAC+S+LS +YI +N+E SDQNFVDEDRE   GSSDC NAKRV  PE N LIN+Q
Sbjct: 181 DSEPLRACDSDLSLQYISSNEEVSDQNFVDEDREVGNGSSDCSNAKRVNTPETNALINEQ 240

[Close]

BLAST of Lag0036952 vs. ExPASy TrEMBL
Match: A0A6J1FBV0 (uncharacterized protein LOC111442637 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442637 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 2.1e-96
Identity = 191/240 (79.58%), Postives = 205/240 (85.42%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLEN  A +TRTDSLISELAKDESTEFALP +SSS GTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHNAVETRTDSLISELAKDESTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESME SFVSQMFDS HSVGS   KENSS TKLHGQSQSASHVHSHF QFKVLRRGSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHSHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFE TESR NLLND+QALSRNPWIHHFRA RKNKNVAC+ QAI S G+N  P GAAN
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDC-NAKRVKIPENNGLINDQ 239
           +S P+RAC+S+LS +YI +N+E SDQNFVDEDRE   GSSDC NAKRV  PE N LIN+Q
Sbjct: 181 DSEPLRACDSDLSLQYISSNEEVSDQNFVDEDREVGNGSSDCSNAKRVNTPETNALINEQ 240

[Close]

BLAST of Lag0036952 vs. ExPASy TrEMBL
Match: A0A6J1IM11 (uncharacterized protein LOC111476815 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476815 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.2e-96
Identity = 188/239 (78.66%), Postives = 202/239 (84.52%), Query Frame = 0


Query: 1   MEFLPRNLENLKARKTRTDSLISELAKDESTEFALPEQSSSQGTHMTESVPSDWTDEKHH 60
           ME LP NLEN  A +TRTD LISEL KD+STEFALP +SSS GTHMT+SVPSDWTDEKHH
Sbjct: 1   MELLPPNLENHIAVETRTDLLISELPKDDSTEFALPVESSSLGTHMTDSVPSDWTDEKHH 60

Query: 61  LFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSW 120
           LFLESME SFVSQMFDS HSVGS   KENSS TKLHGQSQSASHVH+HF QFKVLRRGSW
Sbjct: 61  LFLESMETSFVSQMFDSVHSVGSCPRKENSSHTKLHGQSQSASHVHAHFGQFKVLRRGSW 120

Query: 121 KNINFEPTESRPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAAN 180
           KNINFE TESR NLLND+QALSRNPWIHHFRA RKNKNVAC+ QAI S G+N  P GAAN
Sbjct: 121 KNINFETTESRSNLLNDFQALSRNPWIHHFRADRKNKNVACKSQAIGSGGRNLFPLGAAN 180

Query: 181 NSGPMRACNSNLSQEYI-NNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQ 239
           NS P+RAC S+LS +YI +N+E SDQNFVDEDRE   GSSDCNAKRV  PE N LIN+Q
Sbjct: 181 NSEPLRACGSDLSLQYISSNEEVSDQNFVDEDREVGNGSSDCNAKRVNTPETNALINEQ 239

[Close]

BLAST of Lag0036952 vs. TAIR 10
Match: AT5G42900.1 (cold regulated gene 27 )

HSP 1 Score: 82.0 bits (201), Expect = 8.3e-16
Identity = 72/211 (34.12%), Postives = 109/211 (51.66%), Query Frame = 0


Query: 37  EQSSSQGTHMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLH 96
           E +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S  ++G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSLGALGK---NENVSESTRF 90

Query: 97  GQSQSASHVHSHFSQFKVLRRGSWKNINFEPTESRPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ IN +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RKNKNVAC---RGQAISSRG--KNFLPFGAANNSGPMRACNSNLSQEYINNKEASDQNFV 216
            K +         Q +SS    K     G+A++   + + + +  Q  +   E SDQNFV
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFV 210

Query: 217 DEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
           +E  +GE GSS    K V + E++    DQV
Sbjct: 211 NEGIKGENGSSK-KMKTVMMSESSS--TDQV 229

[Close]

BLAST of Lag0036952 vs. TAIR 10
Match: AT5G42900.2 (cold regulated gene 27 )

HSP 1 Score: 82.0 bits (201), Expect = 8.3e-16
Identity = 72/211 (34.12%), Postives = 109/211 (51.66%), Query Frame = 0


Query: 37  EQSSSQGTHMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLH 96
           E +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S  ++G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSLGALGK---NENVSESTRF 90

Query: 97  GQSQSASHVHSHFSQFKVLRRGSWKNINFEPTESRPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ IN +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RKNKNVAC---RGQAISSRG--KNFLPFGAANNSGPMRACNSNLSQEYINNKEASDQNFV 216
            K +         Q +SS    K     G+A++   + + + +  Q  +   E SDQNFV
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFV 210

Query: 217 DEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
           +E  +GE GSS    K V + E++    DQV
Sbjct: 211 NEGIKGENGSSK-KMKTVMMSESSS--TDQV 229

[Close]

BLAST of Lag0036952 vs. TAIR 10
Match: AT5G42900.3 (cold regulated gene 27 )

HSP 1 Score: 79.3 bits (194), Expect = 5.3e-15
Identity = 72/210 (34.29%), Postives = 108/210 (51.43%), Query Frame = 0


Query: 37  EQSSSQGTHMTESVPSDWTDEKHHLFLESMEASFVSQMFDSAHSVGSGSGKENSSRTKLH 96
           E +SS  +   E + ++WT+EKH L+L+SMEASFV Q+++S  ++G     EN S +   
Sbjct: 31  ETTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQLYNSLGALGK---NENVSESTRF 90

Query: 97  GQSQSASHVHSHFSQFKVLRRGSWKNINFEPTESRPNLL---NDYQALSRNPWIHHFRAA 156
           G  +  S       QFKVL  G W+ IN +  E R N     N ++ L R+PWI H++  
Sbjct: 91  GSGRKPSQ-----EQFKVLHDGFWQKINVKQPEHRINGRHGGNSHEFL-RSPWIKHYKPL 150

Query: 157 RKNKNVAC---RGQAI-SSRGKNFLPFGAANNSGPMRACNSNLSQEYINNKEASDQNFVD 216
            K +         Q + SS GK  +    + +S    + +S    +    +E SDQNFV+
Sbjct: 151 VKTQIPVTDEPENQVVSSSNGKKGICSSGSASSLKQLSSHSRDHDQISVGEEVSDQNFVN 210

Query: 217 EDREGEKGSSDCNAKRVKIPENNGLINDQV 240
           E  +GE GSS    K V + E++    DQV
Sbjct: 211 EGIKGENGSSK-KMKTVMMSESSS--TDQV 228

[Close]

BLAST of Lag0036952 vs. TAIR 10
Match: AT4G33980.1 (BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 59/229 (25.76%), Postives = 91/229 (39.74%), Query Frame = 0


Query: 19  DSLISELAKDESTEFALPEQSSSQGTHMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E  L     S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSWKNINFEPTES 138
           V Q++        G G +  SRT+        S+ H    QF VL+ G W+ +NF   +S
Sbjct: 75  VRQLYSLL-----GGGTQRLSRTR-----DVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAANNSGPMRACNS 198
                     L  +      R + +NK     G                N +        
Sbjct: 135 ---------CLETSSEFRFHRNSLRNKPENSNG----------------NYTMGTTVQGD 194

Query: 199 NLSQEYINNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
            L  +   + EAS QNF +E+ E EKG      +R    +++ L  DQV
Sbjct: 195 VLCHDETKHSEASGQNFREEEEEEEKGEVSKKREREANNDDSSLKEDQV 208

[Close]

BLAST of Lag0036952 vs. TAIR 10
Match: AT4G33980.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.3). )

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 59/229 (25.76%), Postives = 91/229 (39.74%), Query Frame = 0


Query: 19  DSLISELAKDESTEFALPEQSSSQGTHMTESVPSD--------WTDEKHHLFLESMEASF 78
           D  +SE + +  +E  L     S  T  T    +D        WT+EKH+ +L+ +E+SF
Sbjct: 15  DRDVSEASAESQSESTLSNSLDSGVTAETSRSDADSKLDECTAWTNEKHNSYLDYLESSF 74

Query: 79  VSQMFDSAHSVGSGSGKENSSRTKLHGQSQSASHVHSHFSQFKVLRRGSWKNINFEPTES 138
           V Q++        G G +  SRT+        S+ H    QF VL+ G W+ +NF   +S
Sbjct: 75  VRQLYSLL-----GGGTQRLSRTR-----DVQSNSHKSADQFTVLQNGCWQKVNFGKKQS 134

Query: 139 RPNLLNDYQALSRNPWIHHFRAARKNKNVACRGQAISSRGKNFLPFGAANNSGPMRACNS 198
                     L  +      R + +NK     G                N +        
Sbjct: 135 ---------CLETSSEFRFHRNSLRNKPENSNG----------------NYTMGTTVQGD 194

Query: 199 NLSQEYINNKEASDQNFVDEDREGEKGSSDCNAKRVKIPENNGLINDQV 240
            L  +   + EAS QNF +E+ E EKG      +R    +++ L  DQV
Sbjct: 195 VLCHDETKHSEASGQNFREEEEEEEKGEVSKKREREANNDDSSLKEDQV 208

The following BLAST results are available for this feature:

BLAST of Lag0036952 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896198.1	8.3e-103	82.52	cold-regulated protein 27 isoform X1 [Benincasa hispida]	[more]
XP_038896200.1	1.6e-101	82.85	cold-regulated protein 27 isoform X3 [Benincasa hispida]	[more]
XP_038896199.1	1.6e-101	82.85	cold-regulated protein 27 isoform X2 [Benincasa hispida]	[more]
KAG6608473.1	7.8e-101	81.07	Cold-regulated protein 27, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982275.1	1.3e-100	81.07	uncharacterized protein LOC111481154 [Cucurbita maxima]	[more]

BLAST of Lag0036952 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L8T7	1.2e-14	34.12	Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1	[more]
Q8L983	1.7e-05	25.76	Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1	[more]

BLAST of Lag0036952 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J278	6.5e-101	81.07	uncharacterized protein LOC111481154 OS=Cucurbita maxima OX=3661 GN=LOC111481154...	[more]
A0A6J1FJA4	8.7e-98	80.25	uncharacterized protein LOC111445977 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1F6L8	2.1e-96	79.58	uncharacterized protein LOC111442637 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1FBV0	2.1e-96	79.58	uncharacterized protein LOC111442637 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IM11	6.2e-96	78.66	uncharacterized protein LOC111476815 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Lag0036952 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42900.1	8.3e-16	34.12	cold regulated gene 27	[more]
AT5G42900.2	8.3e-16	34.12	cold regulated gene 27	[more]
AT5G42900.3	5.3e-15	34.29	cold regulated gene 27	[more]
AT4G33980.1	1.2e-06	25.76	BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G429...	[more]
AT4G33980.2	1.2e-06	25.76	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOC...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..53
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..108
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..50
None	No IPR available	PANTHER	PTHR33676:SF3	COLD REGULATED PROTEIN 27	coord: 30..245
IPR044678	Cold-regulated protein 27/28	PANTHER	PTHR33676	COLD REGULATED PROTEIN 27	coord: 30..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036952.1	Lag0036952.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042752	regulation of circadian rhythm
biological_process	GO:0009409	response to cold