Lag0036947 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0036947 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 797.7 bits (2059), Expect = 5.0e-227
Identity = 383/449 (85.30%), Postives = 401/449 (89.31%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSP-SSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHV 60
           MGFLASLCFLYL +SLFPSP SSSTLLFQGFNWESSSKGGWYNSL NIIPDL++A+VTHV
Sbjct: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60

Query: 61  WLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELK 120
           WLPPPSQS SSQ                          GYLPGRLYDL+ASKYGTQNELK
Sbjct: 61  WLPPPSQSVSSQ--------------------------GYLPGRLYDLNASKYGTQNELK 120

Query: 121 SLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYS 180
           SLI+ LHEKGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICSDDT+YS
Sbjct: 121 SLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYS 180

Query: 181 DGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKL 240
           DG GN DSGEPY PAPDIDHLNPQVQKE+SEWMRWLKTEIGFDGWRFDFVKGYAPSITKL
Sbjct: 181 DGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKL 240

Query: 241 YMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQ 300
           YMDQTSP FAVGEKWD LSYGQDGKPNQNQD+HR+ALA+WVRASGD VTAFDFTTKGILQ
Sbjct: 241 YMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANWVRASGDIVTAFDFTTKGILQ 300

Query: 301 AAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILT 360
           AAV+GELWRLKDSNG PPGFIGIKPESAVTFI+NHDTGS+QR+WPFPSDKVIQGYAYILT
Sbjct: 301 AAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILT 360

Query: 361 HPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMK 420
           HPGTPSIFYDH VDWGLKEEI KLS IRARNGIGK STVNIMAS+G LYVAGVDGKIIMK
Sbjct: 361 HPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMK 420

Query: 421 IGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           IG N  LGNLIPSDYQLAT GM+YAVWEK
Sbjct: 421 IGANTSLGNLIPSDYQLATYGMDYAVWEK 423

BLAST of Lag0036947 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 794.7 bits (2051), Expect = 4.3e-226
Identity = 379/448 (84.60%), Postives = 400/448 (89.29%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVW 60
           MGFLASLCFLYLS+SLFPSPSSS LLFQGFNWESSSKGGWYNSLKNIIPDLVNA++THVW
Sbjct: 16  MGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNIIPDLVNARITHVW 75

Query: 61  LPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKS 120
           LPPPSQS S Q                          GYLPGRLYDLDASKYGTQNELKS
Sbjct: 76  LPPPSQSVSPQ--------------------------GYLPGRLYDLDASKYGTQNELKS 135

Query: 121 LISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSD 180
           LI+ALHEKGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICSDDTDYSD
Sbjct: 136 LIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSD 195

Query: 181 GTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY 240
           G GN DSGEPY PAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLY
Sbjct: 196 GKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLY 255

Query: 241 MDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQA 300
           MDQTSP FAVGEKWDSLS G DGK NQNQDAHR++LA+WV+ASGD VTAFDFTTKGILQA
Sbjct: 256 MDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQA 315

Query: 301 AVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTH 360
           AVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTGS+Q++WPFPSDKVIQGYAYILTH
Sbjct: 316 AVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTH 375

Query: 361 PGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKI 420
           PGTP+IFYDH V+WGLKEEI +LSEIRARN IGK S VNI+ASD  LYVAGVDGKIIMKI
Sbjct: 376 PGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGKIIMKI 435

Query: 421 GPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           G N +LGNLIPS++QLATSGMEYAVWEK
Sbjct: 436 GANTNLGNLIPSEFQLATSGMEYAVWEK 437

BLAST of Lag0036947 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 779.6 bits (2012), Expect = 1.4e-221
Identity = 375/450 (83.33%), Postives = 397/450 (88.22%), Query Frame = 0

Query: 1   MGFLASLCFL-YLSISLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTH 60
           MGFLASLCFL YLS+ LFPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDLV+AKVTH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNEL 120
           VWLPPPSQSAS Q                          GYLPGRLYDL+ASKYGTQNEL
Sbjct: 85  VWLPPPSQSASPQ--------------------------GYLPGRLYDLNASKYGTQNEL 144

Query: 121 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 180
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 145 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 204

Query: 181 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 240
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 205 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 264

Query: 241 LYMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGIL 300
           LYMDQTSP FAVGEKWDSLS G DGKP+ NQD HR ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 265 LYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 324

Query: 301 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYIL 360
           QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGS+Q++WPFPSDKVIQGYAYIL
Sbjct: 325 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 384

Query: 361 THPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 420
           THPGTPSIFYDH V+WGLKEE+ KLS IRARNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 385 THPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIM 444

Query: 421 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           KIGPN +LGNL+PSDY+LA SGM+YAVWEK
Sbjct: 445 KIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

BLAST of Lag0036947 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 776.2 bits (2003), Expect = 1.6e-220
Identity = 374/450 (83.11%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MGFLASLCFL-YLSISLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTH 60
           MGFLASLCFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDLV+AKVTH
Sbjct: 43  MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 102

Query: 61  VWLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNEL 120
           VWLPPPSQSAS Q                          GYLPGRLYDL+ASKYGTQ+EL
Sbjct: 103 VWLPPPSQSASPQ--------------------------GYLPGRLYDLNASKYGTQDEL 162

Query: 121 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 180
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 163 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 222

Query: 181 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 240
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 223 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 282

Query: 241 LYMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGIL 300
           LYMDQTSP FAVGEKWDSLS G DGKP+ NQD HR ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 283 LYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 342

Query: 301 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYIL 360
           QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGS+Q++WPFPSDKVIQGYAYIL
Sbjct: 343 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 402

Query: 361 THPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 420
           THPGTPSIFYDH V+WGLKEE+ KLS IR RNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 403 THPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIM 462

Query: 421 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           KIGPN +LGNLIPSDY LATSGM+YAVWEK
Sbjct: 463 KIGPNTNLGNLIPSDYVLATSGMQYAVWEK 466

BLAST of Lag0036947 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 775.4 bits (2001), Expect = 2.7e-220
Identity = 373/450 (82.89%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MGFLASLCFL-YLSISLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTH 60
           MGFLASLCFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDLV+AKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNEL 120
           VWLPPPSQSAS Q                          GYLPGRLYDL+ASKYGTQ+EL
Sbjct: 61  VWLPPPSQSASPQ--------------------------GYLPGRLYDLNASKYGTQDEL 120

Query: 121 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 180
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 121 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 180

Query: 181 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 240
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 181 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 240

Query: 241 LYMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGIL 300
           LYMDQTSP FAVGEKWDSLS G DGKP+ NQD HR ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 241 LYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 300

Query: 301 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYIL 360
           QAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTGS+Q++WPFPSDKVIQGYAYIL
Sbjct: 301 QAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 360

Query: 361 THPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 420
           THPGTPSIFYDH V+WGLKEE+ KLS IR RNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 361 THPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIM 420

Query: 421 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           KIGPN +LGNLIPSDY+LATSGM+YAVWEK
Sbjct: 421 KIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

BLAST of Lag0036947 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 9.3e-200
Identity = 329/447 (73.60%), Postives = 365/447 (81.66%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVW 60
           M   + L    L ISL P  SS  LLFQGFNWESS KGGWYNSLKN IPDL NA +THVW
Sbjct: 1   MDSFSRLSIFCLFISLLPLFSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVW 60

Query: 61  LPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKS 120
           LPPPSQS S +                          GYLPGRLYDLDASKYG++NELKS
Sbjct: 61  LPPPSQSVSPE--------------------------GYLPGRLYDLDASKYGSKNELKS 120

Query: 121 LISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSD 180
           LI+A HEKGIK +ADIVINHRTAE++D RGI+CIFEGGTPD R DWGPSFIC DDT YSD
Sbjct: 121 LIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSFICRDDTAYSD 180

Query: 181 GTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY 240
           GTGN DSGE Y  APDIDHLNPQVQ+E+SEWM WLKTEIGFDGWRFDFVKGYAPSI+K+Y
Sbjct: 181 GTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVKGYAPSISKIY 240

Query: 241 MDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQA 300
           M+QT P FAVGEKWDS+SYGQDGKPN NQD+HR AL +WV ++G  +TAFDFTTKGILQA
Sbjct: 241 MEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAFDFTTKGILQA 300

Query: 301 AVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTH 360
           AVQGELWRL D NGKPPG IG+KPE+AVTFIDNHDTGS+QR+WPFPSDKV+QGYAYILTH
Sbjct: 301 AVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTH 360

Query: 361 PGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKI 420
           PGTPSIFYDH  DWGLKE+I+KLS IR RNGI +KSTV IMAS+G LYVA +D KI++KI
Sbjct: 361 PGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYVAKIDNKIMVKI 420

Query: 421 GPNMDLGNLIPSDYQLATSGMEYAVWE 448
           GP MDLGNLIPS+  +ATSG +YAVWE
Sbjct: 421 GPKMDLGNLIPSNLHVATSGQDYAVWE 421

BLAST of Lag0036947 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 9.4e-160
Identity = 254/442 (57.47%), Postives = 321/442 (72.62%), Query Frame = 0

Query: 8   CFLYLSISLFPSPSSSTLLFQGFNWESSSK-GGWYNSLKNIIPDLVNAKVTHVWLPPPSQ 67
           C ++  + L  S + + +LFQGFNWES  K GGWYN L   + ++ +   THVWLPPPS 
Sbjct: 10  CVVFAVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSH 69

Query: 68  SASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISALH 127
           S S Q                          GY+PGRLYDLDASKYGT+ ELKSLI A H
Sbjct: 70  SVSPQ--------------------------GYMPGRLYDLDASKYGTEAELKSLIEAFH 129

Query: 128 EKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRD 187
           +K ++ +ADIVINHR A+ +D RG++C+FEGGTPDGRLDWGP  ICSDDT YS+G G+RD
Sbjct: 130 DKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRD 189

Query: 188 SGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSP 247
           +G  +  APDIDHLNP+VQ+E+++W+ WL+T++GFDGWR DF KGY+  + ++Y+D T+P
Sbjct: 190 TGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNP 249

Query: 248 YFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQAAVQGEL 307
            F VGE W SL Y  DGKP+ NQDA R  L +WV   G   TAFDFTTKGILQAAVQGEL
Sbjct: 250 TFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFDFTTKGILQAAVQGEL 309

Query: 308 WRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPGTPSI 367
           WRL D NGK PG +G  P+ AVTF+DNHDTGS+Q +WPFPSDKV+QGYAYILTHPG P I
Sbjct: 310 WRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCI 369

Query: 368 FYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDL 427
           FYDH+ DW L+ EI+ L+EIR+RNGI  +ST++I+ ++G +YVA +DGK+I K+GP  D 
Sbjct: 370 FYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDA 425

Query: 428 GNLIPSDYQLATSGMEYAVWEK 449
           G +IPSD+ +   G +Y VWEK
Sbjct: 430 GGIIPSDFHVVAHGNDYCVWEK 425

BLAST of Lag0036947 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 560.8 bits (1444), Expect = 1.4e-158
Identity = 260/447 (58.17%), Postives = 318/447 (71.14%), Query Frame = 0

Query: 5   ASLCFLYLSI--SLFPSPSSSTLLFQGFNWES-SSKGGWYNSLKNIIPDLVNAKVTHVWL 64
           +SLC L L +  SL  +   + +LFQGFNWES   +GGWYN LK  + D+  A VTHVWL
Sbjct: 5   SSLCLLLLVVLCSLTCNSGQAQVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVTHVWL 64

Query: 65  PPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSL 124
           PPPS S + Q                          GY+PGRLYDLDASKYGT  ELKSL
Sbjct: 65  PPPSHSVAPQ--------------------------GYMPGRLYDLDASKYGTAAELKSL 124

Query: 125 ISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDG 184
           I+A H KG++ VAD+VINHR AEK+D RG++C+FEGGTPD RLDWGP  ICSDDT YSDG
Sbjct: 125 IAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDDRLDWGPGMICSDDTQYSDG 184

Query: 185 TGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYM 244
           TG+RD+GE +  APDIDHLNP+VQ+E+++W+ WLK+++GFDGWR DF KGY+  I K+Y+
Sbjct: 185 TGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKSDVGFDGWRLDFAKGYSTDIAKMYV 244

Query: 245 DQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQAA 304
           +   P F V E W+SLSY  DGKP  NQD  R  L +WV A G     FDFTTKG+LQA 
Sbjct: 245 ESCKPGFVVAEIWNSLSYNGDGKPAANQDQGRQELVNWVNAVGGPAMTFDFTTKGLLQAG 304

Query: 305 VQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHP 364
           VQGELWRL+D NGK  G IG  PE AVTF+DNHDTGS+Q++WPFPSDKV+QGYAYILTHP
Sbjct: 305 VQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTGSTQKLWPFPSDKVMQGYAYILTHP 364

Query: 365 GTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIG 424
           G P IFYDH+ DW LK+EI+ L+ IR RNGI   S + I+ +D   YVA VD K+++KIG
Sbjct: 365 GVPCIFYDHMFDWNLKQEITALAAIRERNGINAGSKLRIVVADADAYVAVVDEKVMVKIG 424

Query: 425 PNMDLGNLIPSDYQLATSGMEYAVWEK 449
              D+GN +PSD+     G +Y+VWEK
Sbjct: 425 TRYDVGNAVPSDFHQTVHGKDYSVWEK 425

BLAST of Lag0036947 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 557.4 bits (1435), Expect = 1.5e-157
Identity = 261/446 (58.52%), Postives = 317/446 (71.08%), Query Frame = 0

Query: 6   SLCFLYLSISLFPSPSSSTLLFQGFNWES-SSKGGWYNSLKNIIPDLVNAKVTHVWLPPP 65
           SL    + + L  S +S  +LFQGFNWES    GGWYN L   + D+  A +THVWLPP 
Sbjct: 7   SLSLFLVLLGLSASLASGQVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLPPA 66

Query: 66  SQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISA 125
           SQS + Q                          GY+PGRLYDLDASKYG + +LKSLI A
Sbjct: 67  SQSVAEQ--------------------------GYMPGRLYDLDASKYGNKAQLKSLIGA 126

Query: 126 LHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGN 185
           LH KG+KA+ADIVINHRTAE +D RGI+CIFEGGTPD RLDWGP  IC DD  Y+DGTGN
Sbjct: 127 LHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGN 186

Query: 186 RDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQT 245
            D+G  +  APDIDHLN +VQKE+ EW+ WLK +IGFDGWRFDF KGY+  + K+Y+D++
Sbjct: 187 PDTGADFGAAPDIDHLNLRVQKELVEWLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRS 246

Query: 246 SPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASG--DTVTAFDFTTKGILQAAV 305
            P FAV E W SL+YG DGKPN NQD HR  L +WV   G     T FDFTTKGIL  AV
Sbjct: 247 EPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNVAV 306

Query: 306 QGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPG 365
           +GELWRL+ ++GK PG IG  P  AVTF+DNHDTGS+Q +WPFPSD+V+QGYAYILTHPG
Sbjct: 307 EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPG 366

Query: 366 TPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGP 425
           TP IFYDH  DWGLKEEI +L  +R R+GI  +S + I+ +D  LY+A +DGK+I+K+GP
Sbjct: 367 TPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKLGP 426

Query: 426 NMDLGNLIPSDYQLATSGMEYAVWEK 449
             D+GNLIP  +++A  G +YAVWEK
Sbjct: 427 RYDVGNLIPGGFKVAAHGNDYAVWEK 426

BLAST of Lag0036947 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.7e-156
Identity = 266/429 (62.00%), Postives = 322/429 (75.06%), Query Frame = 0

Query: 22  SSTLLFQGFNWESSSK-GGWYNSLKNIIPDLVNAKVTHVWLPPPSQSASSQGKLLSLFLQ 81
           SSTLLFQ FNWES  K GG+YNSL N I D+ NA +TH+WLPPPSQS + +         
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPE--------- 83

Query: 82  LIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINH 141
                            GYLPG+LYDL++SKYG++ ELKSLI AL++KGIKA+ADIVINH
Sbjct: 84  -----------------GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINH 143

Query: 142 RTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHL 201
           RTAE++D +  +C FEGGT D RLDW PSF+C +D  +  GTGN D+G  +  APDIDHL
Sbjct: 144 RTAERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHL 203

Query: 202 NPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPYFAVGEKWDSLSYG 261
           NP+VQKE+SEWM WLKTEIGF GWRFD+V+GYA SITKLY+  TSP FAVGEKWD + YG
Sbjct: 204 NPRVQKELSEWMNWLKTEIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYG 263

Query: 262 QDGKPNQNQDAHRNALADWV-RASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGF 321
            DGK + +Q+ HR+ L  W+  A G  +TAFDFTTKGILQ+AV+GELWRLKDS GKPPG 
Sbjct: 264 GDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGM 323

Query: 322 IGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPGTPSIFYDHLVDWGLKEE 381
           IGI P +AVTFIDNHDT    R W FPSDKV+ GY YILTHPGTP IFY+H ++WGLKE 
Sbjct: 324 IGIMPGNAVTFIDNHDT---FRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKES 383

Query: 382 ISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATS 441
           ISKL  IR +NGIG  S+V I A++  LY+A +D K+IMKIGP  D+G L+PS++ LA S
Sbjct: 384 ISKLVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYS 422

Query: 442 GMEYAVWEK 449
           G+++AVWEK
Sbjct: 444 GLDFAVWEK 422

BLAST of Lag0036947 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 379/448 (84.60%), Postives = 400/448 (89.29%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVW 60
           MGFLASLCFLYLS+SLFPSPSSS LLFQGFNWESSSKGGWYNSLKNIIPDLVNA++THVW
Sbjct: 16  MGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNIIPDLVNARITHVW 75

Query: 61  LPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKS 120
           LPPPSQS S Q                          GYLPGRLYDLDASKYGTQNELKS
Sbjct: 76  LPPPSQSVSPQ--------------------------GYLPGRLYDLDASKYGTQNELKS 135

Query: 121 LISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSD 180
           LI+ALHEKGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICSDDTDYSD
Sbjct: 136 LIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSD 195

Query: 181 GTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY 240
           G GN DSGEPY PAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLY
Sbjct: 196 GKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLY 255

Query: 241 MDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQA 300
           MDQTSP FAVGEKWDSLS G DGK NQNQDAHR++LA+WV+ASGD VTAFDFTTKGILQA
Sbjct: 256 MDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQA 315

Query: 301 AVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTH 360
           AVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTGS+Q++WPFPSDKVIQGYAYILTH
Sbjct: 316 AVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTH 375

Query: 361 PGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKI 420
           PGTP+IFYDH V+WGLKEEI +LSEIRARN IGK S VNI+ASD  LYVAGVDGKIIMKI
Sbjct: 376 PGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVDGKIIMKI 435

Query: 421 GPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           G N +LGNLIPS++QLATSGMEYAVWEK
Sbjct: 436 GANTNLGNLIPSEFQLATSGMEYAVWEK 437

BLAST of Lag0036947 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.9e-222
Identity = 375/450 (83.33%), Postives = 397/450 (88.22%), Query Frame = 0

Query: 1   MGFLASLCFL-YLSISLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTH 60
           MGFLASLCFL YLS+ LFPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDLV+AKVTH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNEL 120
           VWLPPPSQSAS Q                          GYLPGRLYDL+ASKYGTQNEL
Sbjct: 85  VWLPPPSQSASPQ--------------------------GYLPGRLYDLNASKYGTQNEL 144

Query: 121 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 180
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 145 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 204

Query: 181 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 240
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 205 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 264

Query: 241 LYMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGIL 300
           LYMDQTSP FAVGEKWDSLS G DGKP+ NQD HR ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 265 LYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 324

Query: 301 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYIL 360
           QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGS+Q++WPFPSDKVIQGYAYIL
Sbjct: 325 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 384

Query: 361 THPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 420
           THPGTPSIFYDH V+WGLKEE+ KLS IRARNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 385 THPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIM 444

Query: 421 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           KIGPN +LGNL+PSDY+LA SGM+YAVWEK
Sbjct: 445 KIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

BLAST of Lag0036947 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 373/450 (82.89%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MGFLASLCFL-YLSISLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTH 60
           MGFLASLCFL YLS+ +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDLV+AKVTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNEL 120
           VWLPPPSQSAS Q                          GYLPGRLYDL+ASKYGTQ+EL
Sbjct: 61  VWLPPPSQSASPQ--------------------------GYLPGRLYDLNASKYGTQDEL 120

Query: 121 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 180
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 121 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 180

Query: 181 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 240
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 181 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 240

Query: 241 LYMDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGIL 300
           LYMDQTSP FAVGEKWDSLS G DGKP+ NQD HR ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 241 LYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 300

Query: 301 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYIL 360
           QAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTGS+Q++WPFPSDKVIQGYAYIL
Sbjct: 301 QAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 360

Query: 361 THPGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 420
           THPGTPSIFYDH V+WGLKEE+ KLS IR RNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 361 THPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIM 420

Query: 421 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           KIGPN +LGNLIPSDY+LATSGM+YAVWEK
Sbjct: 421 KIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

BLAST of Lag0036947 vs. ExPASy TrEMBL
Match: A0A2N9FNN7 (Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.9e-202
Identity = 336/448 (75.00%), Postives = 373/448 (83.26%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVW 60
           M  L SLCFL L ISL PS +   +LFQGFNWESS+KGGWYN LKN IPDL NA +THVW
Sbjct: 1   MNILTSLCFLCLCISLSPSFAVPAILFQGFNWESSNKGGWYNLLKNSIPDLANAGITHVW 60

Query: 61  LPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKS 120
           LPPPSQS + Q                          GY+PGRLYDLDASKYG+++ELKS
Sbjct: 61  LPPPSQSVAPQ--------------------------GYMPGRLYDLDASKYGSKDELKS 120

Query: 121 LISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSD 180
           LI+ALHEKGIKA+ADIVINHRTAEKQD RGIWCIFEGGTPDGRLDWGPSFIC DDT YSD
Sbjct: 121 LIAALHEKGIKALADIVINHRTAEKQDGRGIWCIFEGGTPDGRLDWGPSFICKDDTAYSD 180

Query: 181 GTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY 240
           GTGN DSG+ +  APDIDH+NPQVQKE+SEWM WLKTEIGFDGWRFDFV GYA S TK+Y
Sbjct: 181 GTGNPDSGKDFGGAPDIDHVNPQVQKELSEWMNWLKTEIGFDGWRFDFVLGYASSFTKVY 240

Query: 241 MDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQA 300
           M+QT P FAVGE W+SLSYG  GKPN NQDAHRNAL DW++A+G  VTAFDFTTKGILQA
Sbjct: 241 MEQTKPDFAVGENWNSLSYGDGGKPNPNQDAHRNALVDWIQAAGGAVTAFDFTTKGILQA 300

Query: 301 AVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTH 360
           AV+GELWRLKDSNGKPPG IGIKPE+AVTFIDNHDTGS+Q++WPFPSDKVIQGYAYILTH
Sbjct: 301 AVEGELWRLKDSNGKPPGLIGIKPENAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTH 360

Query: 361 PGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKI 420
           PGTPSIFYDHL DWGLK+EISKL+ IR+RNGI  +  VNI+ASD  LYVAG+D KIIMKI
Sbjct: 361 PGTPSIFYDHLFDWGLKDEISKLTAIRSRNGINTQGIVNILASDADLYVAGIDDKIIMKI 420

Query: 421 GPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           GP MDLGNLIPS++Q++ SG +YAVWEK
Sbjct: 421 GPKMDLGNLIPSNFQVSASGKDYAVWEK 422

BLAST of Lag0036947 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.5e-200
Identity = 335/448 (74.78%), Postives = 371/448 (82.81%), Query Frame = 0

Query: 1   MGFLASLCFLYLSISLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVW 60
           M     LCFL L ISL  S ++ +LLFQGFNWESS KGGWYNSLKN +PDL NA++THVW
Sbjct: 1   MNLKTFLCFLCLFISLCHSFAAPSLLFQGFNWESSKKGGWYNSLKNFVPDLANAEITHVW 60

Query: 61  LPPPSQSASSQGKLLSLFLQLIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKS 120
           LPPPSQSA+ +                          GYLPGRLYDLD SKYG+++ELK+
Sbjct: 61  LPPPSQSAAPE--------------------------GYLPGRLYDLDVSKYGSKDELKA 120

Query: 121 LISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSD 180
           LI+ALHEKGIK VADIVINHRTAEKQD RGIW IFEGGTPD RLDWGPSFIC DD  YSD
Sbjct: 121 LIAALHEKGIKVVADIVINHRTAEKQDDRGIWSIFEGGTPDARLDWGPSFICKDDIAYSD 180

Query: 181 GTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLY 240
           GTGN DSGE Y PAPDIDH NPQVQKE+SEWM WLKTEIG+DGWRFDFVKGYAPSI K Y
Sbjct: 181 GTGNPDSGEGYDPAPDIDHRNPQVQKELSEWMNWLKTEIGYDGWRFDFVKGYAPSIMKNY 240

Query: 241 MDQTSPYFAVGEKWDSLSYGQDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQA 300
           M+QT P FAVGE W+SL+YG DGKPN NQDAHR ALA+WV ++G  VTAFDFTTKGILQA
Sbjct: 241 MEQTKPDFAVGENWNSLAYGDDGKPNPNQDAHRGALAEWVGSAGGAVTAFDFTTKGILQA 300

Query: 301 AVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTH 360
           AV+GELWRLKDSNGKPPG IGIKPESAVTFIDNHDTGS+Q IWPFPSDKV+QGYAYILTH
Sbjct: 301 AVEGELWRLKDSNGKPPGLIGIKPESAVTFIDNHDTGSTQNIWPFPSDKVMQGYAYILTH 360

Query: 361 PGTPSIFYDHLVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKI 420
           PGTP+IFYDH  DWGLKEEISKL+ IR+RNGI  +S V+I+ASDG LYVAG+D KIIMKI
Sbjct: 361 PGTPTIFYDHFFDWGLKEEISKLTAIRSRNGINTRSIVSILASDGDLYVAGIDDKIIMKI 420

Query: 421 GPNMDLGNLIPSDYQLATSGMEYAVWEK 449
           GP MDLGNLIPS++Q++TSG +YAVWEK
Sbjct: 421 GPKMDLGNLIPSNFQVSTSGKDYAVWEK 422

BLAST of Lag0036947 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 266/429 (62.00%), Postives = 322/429 (75.06%), Query Frame = 0

Query: 22  SSTLLFQGFNWESSSK-GGWYNSLKNIIPDLVNAKVTHVWLPPPSQSASSQGKLLSLFLQ 81
           SSTLLFQ FNWES  K GG+YNSL N I D+ NA +TH+WLPPPSQS + +         
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPE--------- 83

Query: 82  LIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINH 141
                            GYLPG+LYDL++SKYG++ ELKSLI AL++KGIKA+ADIVINH
Sbjct: 84  -----------------GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINH 143

Query: 142 RTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHL 201
           RTAE++D +  +C FEGGT D RLDW PSF+C +D  +  GTGN D+G  +  APDIDHL
Sbjct: 144 RTAERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHL 203

Query: 202 NPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPYFAVGEKWDSLSYG 261
           NP+VQKE+SEWM WLKTEIGF GWRFD+V+GYA SITKLY+  TSP FAVGEKWD + YG
Sbjct: 204 NPRVQKELSEWMNWLKTEIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYG 263

Query: 262 QDGKPNQNQDAHRNALADWV-RASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGF 321
            DGK + +Q+ HR+ L  W+  A G  +TAFDFTTKGILQ+AV+GELWRLKDS GKPPG 
Sbjct: 264 GDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGM 323

Query: 322 IGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPGTPSIFYDHLVDWGLKEE 381
           IGI P +AVTFIDNHDT    R W FPSDKV+ GY YILTHPGTP IFY+H ++WGLKE 
Sbjct: 324 IGIMPGNAVTFIDNHDT---FRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKES 383

Query: 382 ISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATS 441
           ISKL  IR +NGIG  S+V I A++  LY+A +D K+IMKIGP  D+G L+PS++ LA S
Sbjct: 384 ISKLVAIRNKNGIGSTSSVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYS 422

Query: 442 GMEYAVWEK 449
           G+++AVWEK
Sbjct: 444 GLDFAVWEK 422

BLAST of Lag0036947 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 370.2 bits (949), Expect = 2.4e-102
Identity = 188/428 (43.93%), Postives = 253/428 (59.11%), Query Frame = 0

Query: 21  SSSTLLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVWLPPPSQSASSQGKLLSLFLQ 80
           S   +L QGFNWES+  G WY  L+    +L +   T +WLPPP++S S +         
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPE--------- 552

Query: 81  LIIKNISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINH 140
                            GY+P  LY+L+ S+YGT +ELK  +   H+ GIK + D V+NH
Sbjct: 553 -----------------GYMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNH 612

Query: 141 RTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHL 200
           R A  ++Q G+W +F      GRL+W    + +DD  +  G GN+ SG+ +  AP+IDH 
Sbjct: 613 RCAHFKNQNGVWNLF-----GGRLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHS 672

Query: 201 NPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPYFAVGEKWDSLSYG 260
              V+K+I EW+ W+  E+G+DGWR DFV+G+     K YMD + PYFAVGE WDSLSY 
Sbjct: 673 QDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYT 732

Query: 261 QDGKPNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQAAVQG-ELWRLKDSNGKPPGF 320
             G+ + NQDAHR  + DW+ A+     AFD TTKGIL  A+Q  E WRL D  GKPPG 
Sbjct: 733 Y-GEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGV 792

Query: 321 IGIKPESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPGTPSIFYDHLVDWGLKEE 380
           +G  P  AVTFI+NHDTGS+Q  W FP  K +QGYAYILTHPGTP++F+DH+       E
Sbjct: 793 VGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFS-DYHSE 852

Query: 381 ISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATS 440
           I+ L  +R R  +  +S VNI  S+  +Y A +D K+ MKIGP          ++ +A  
Sbjct: 853 IAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVE 885

Query: 441 GMEYAVWE 448
           G +Y VWE
Sbjct: 913 GRDYKVWE 885

BLAST of Lag0036947 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 358.6 bits (919), Expect = 7.3e-99
Identity = 184/428 (42.99%), Postives = 255/428 (59.58%), Query Frame = 0

Query: 25  LLFQGFNWESSSKGGWYNSLKNIIPDLVNAKVTHVWLPPPSQSASSQGKLLSLFLQLIIK 84
           ++ Q +NWE S K  W+ +L   +PD+  +  T  WLPPPSQS + +             
Sbjct: 27  VILQAYNWE-SHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPE------------- 86

Query: 85  NISLNLCCSKPFTGYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAE 144
                        GYLP  LY L+ S YG+++ LKSL+  + +  ++A+ADIVINHR   
Sbjct: 87  -------------GYLPQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGT 146

Query: 145 KQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQV 204
            +   G++  ++G +    L W    +    T  + G GNR +G+ +   P++DH    V
Sbjct: 147 TRGHGGMYNRYDGIS----LPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDHTQHFV 206

Query: 205 QKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPYFAVGEKWDSLSYGQDGK 264
           +K+I  W+RWL+  +GF  +RFDF +GY+ +  K Y+    P F+VGE WDS +Y   G 
Sbjct: 207 RKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL 266

Query: 265 PNQNQDAHRNALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324
            + NQD+HR  +  W+ A+G    AFDFTTKGILQ AV+G+ WRL D+ GKPPG +G  P
Sbjct: 267 -DYNQDSHRQRIISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWP 326

Query: 325 ESAVTFIDNHDTGSSQRIWPFPSDKVIQGYAYILTHPGTPSIFYDHLVDWG--LKEEISK 384
             AVTF+DNHDTGS+Q  WPFPS  V++GYAYILTHPG P +FYDH  DWG  + ++I K
Sbjct: 327 SRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVK 386

Query: 385 LSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPS--DYQLATSG 444
           L +IR R  I  +STV ++ ++  LY A V  KI MK+G     G+  PS  D+ LATSG
Sbjct: 387 LIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGD----GSWCPSGRDWTLATSG 413

Query: 445 MEYAVWEK 449
             YAVW K
Sbjct: 447 HRYAVWHK 413