Lag0036937 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0036937 vs. NCBI nr
Match: XP_038898530.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 301.2 bits (770), Expect = 5.7e-78
Identity = 139/163 (85.28%), Postives = 158/163 (96.93%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           AADSN+PELD+ELLPYL+LY+NGVVERL+GTR+TPPGLD++TGVDSKDI+IVPETGVSAR
Sbjct: 2   AADSNNPELDLELLPYLRLYRNGVVERLLGTRITPPGLDTRTGVDSKDIIIVPETGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPTAV+P RKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE ++V++SVNYRLAPE
Sbjct: 62  LYRPTAVDPHRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAQTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           HPLPAAYDDSWAALQWIAAQSQ+S D+  HEPWLK+LVDFEKV
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQTSADEPGHEPWLKELVDFEKV 164

BLAST of Lag0036937 vs. NCBI nr
Match: XP_008452527.1 (PREDICTED: probable carboxylesterase 2 [Cucumis melo] >KAA0064370.1 putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 297.0 bits (759), Expect = 1.1e-76
Identity = 139/163 (85.28%), Postives = 154/163 (94.48%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A DSN+PEL VELLPYL+LYKNG+V+RLIGTRVTPPGLDS+TGVDSKDI+IVP+TGVSAR
Sbjct: 2   ATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPT V+PRRKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE  +V++SVNYRLAPE
Sbjct: 62  LYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           HPLPAAYDDSWAALQWIAAQSQSS D   HEPWL++LVDFEKV
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKV 164

BLAST of Lag0036937 vs. NCBI nr
Match: XP_011654101.1 (probable carboxylesterase 2 [Cucumis sativus] >KGN55177.1 hypothetical protein Csa_012076 [Cucumis sativus])

HSP 1 Score: 296.2 bits (757), Expect = 1.8e-76
Identity = 139/163 (85.28%), Postives = 155/163 (95.09%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A D N+PELDVELLPYL+LYKNGVVERL+GTRVTPPGLDS+TGV SKDIVIVP+TGVSAR
Sbjct: 2   ATDLNNPELDVELLPYLRLYKNGVVERLLGTRVTPPGLDSRTGVHSKDIVIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPTAV+P RKLPLVVYFHGGAF++ASSAEP+YHNNCLIPLAAE ++V++SVNYRLAPE
Sbjct: 62  LYRPTAVDPGRKLPLVVYFHGGAFLVASSAEPVYHNNCLIPLAAEAQTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           HPLPAAYDDSWAALQWIAAQS+SS D+  HEPWLK+LVDFEKV
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSKSSADEPGHEPWLKELVDFEKV 164

BLAST of Lag0036937 vs. NCBI nr
Match: TYK20217.1 (putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 296.2 bits (757), Expect = 1.8e-76
Identity = 138/163 (84.66%), Postives = 154/163 (94.48%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A DSN+PEL VELLPYL+LYKNG+V+RL+GTRVTPPGLDS+TGVDSKDI+IVP+TGVSAR
Sbjct: 2   ATDSNNPELAVELLPYLRLYKNGIVDRLLGTRVTPPGLDSRTGVDSKDIIIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPT V+PRRKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE  +V++SVNYRLAPE
Sbjct: 62  LYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           HPLPAAYDDSWAALQWIAAQSQSS D   HEPWL++LVDFEKV
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKV 164

BLAST of Lag0036937 vs. NCBI nr
Match: XP_022982269.1 (probable carboxylesterase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 290.8 bits (743), Expect = 7.7e-75
Identity = 139/167 (83.23%), Postives = 154/167 (92.22%), Query Frame = 0

Query: 1   MAAADSNDP--ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETG 60
           M A DSN+P  EL++ELLPYL++YKNGVVERL+GTRVTPPGLDS TGVDSKDI IVPETG
Sbjct: 1   MVAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETG 60

Query: 61  VSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYR 120
           +SARLYRPTAVEP RKLPL+VYFHGGAF+I SSAEPIYHNNCLIPLAA  ESVV+SVNYR
Sbjct: 61  ISARLYRPTAVEPPRKLPLIVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYR 120

Query: 121 LAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           LAPEHPLPAAYDDSWAALQW+A QS++SGD+A  EPWLKQLVDF+KV
Sbjct: 121 LAPEHPLPAAYDDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFKKV 167

BLAST of Lag0036937 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 2.2e-40
Identity = 80/161 (49.69%), Postives = 113/161 (70.19%), Query Frame = 0

Query: 5   DSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLY 64
           +S   ++ +ELLP+L ++ +G VERL GT V PPGLD  TGV SKDI+I P+TG+SAR+Y
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 65  RPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHP 124
           RP +++P +K+PL++YFHGGAF+I+S++ P YH + L  +  +   + VSVNYRLAPEHP
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTS-LNKIVNQANVIAVSVNYRLAPEHP 121

Query: 125 LPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKV 166
           LP AY+DSW AL+ I          A +EPW+    D + +
Sbjct: 122 LPTAYEDSWTALKNI---------QAINEPWINDYADLDSL 152

BLAST of Lag0036937 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.2e-34
Identity = 77/162 (47.53%), Postives = 105/162 (64.81%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPT 67
           D E+ ++L P+L +YK+G +ER +GT V P    +   V +KD+VI P TGVS RLY P 
Sbjct: 80  DDEIVLDLKPFLIIYKSGRIERFLGTTVIP----ACPEVATKDVVIDPATGVSVRLYLPN 139

Query: 68  AVE-PRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 127
            V+ P +KLP++VYFHGG FVI ++  P YH N L  LAA+   ++VS+NYRLAPE+PLP
Sbjct: 140 VVDLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLP 199

Query: 128 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVLFT 169
           A+YDD  A   W+ + S         EPWL Q  DF ++L +
Sbjct: 200 ASYDDCMAGFNWVVSHSAGPA----LEPWLAQHGDFSQILLS 232

BLAST of Lag0036937 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.2e-34
Identity = 77/162 (47.53%), Postives = 105/162 (64.81%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPT 67
           D E+ ++L P+L +YK+G +ER +GT V P    +   V +KD+VI P TGVS RLY P 
Sbjct: 77  DDEIVLDLKPFLIIYKSGRIERFLGTTVIP----ACPEVATKDVVIDPATGVSVRLYLPN 136

Query: 68  AVE-PRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 127
            V+ P +KLP++VYFHGG FVI ++  P YH N L  LAA+   ++VS+NYRLAPE+PLP
Sbjct: 137 VVDLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLP 196

Query: 128 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVLFT 169
           A+YDD  A   W+ + S         EPWL Q  DF ++L +
Sbjct: 197 ASYDDCMAGFNWVVSHSAGPA----LEPWLAQHGDFSQILLS 229

BLAST of Lag0036937 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-33
Identity = 71/164 (43.29%), Postives = 103/164 (62.80%), Query Frame = 0

Query: 2   AAADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPG-LDSQTGVDSKDIVIVPETGVS 61
           + + +   E+D EL P L++YK+G VER +G+   PP   D +TGV +KDIVI     +S
Sbjct: 4   STSTTTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTIS 63

Query: 62  ARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLA 121
           AR+Y P       KLP++VY+HGGAF +  SA    H   L  +A++   +VVS+ YRLA
Sbjct: 64  ARVYLPKLNNTTEKLPILVYYHGGAFCL-ESAFSFLHQRYLNIVASKANVLVVSIEYRLA 123

Query: 122 PEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEK 165
           PEHPLPAAY+D W AL+W+ + S ++    + +PWL +  DF +
Sbjct: 124 PEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNR 166

BLAST of Lag0036937 vs. ExPASy Swiss-Prot
Match: R4X244 (Tuliposide A-converting enzyme b1, amyloplastic OS=Tulipa gesneriana OX=13306 GN=TCEA-B1 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.4e-33
Identity = 75/162 (46.30%), Postives = 105/162 (64.81%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPT 67
           D E++++L P+L +YK+G +ERL GT V P    +   V +KD++I P TGVS RLY P 
Sbjct: 71  DDEIELDLSPFLIIYKDGRIERLKGTTVIP----ACPEVATKDVIIDPATGVSVRLYLPN 130

Query: 68  AVE-PRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 127
            V+ P +KLP++VYFHGG FVI ++  P YH N L  LAA+   ++VSVNYRLAPEHP+P
Sbjct: 131 VVDLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKSGLLIVSVNYRLAPEHPIP 190

Query: 128 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVLFT 169
           A++DD  A   W+ + S         EPWL +  D  ++L +
Sbjct: 191 ASFDDCMAGFNWVVSHSAGPAP----EPWLARHGDLTQILIS 223