Lag0036435 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036435
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionExonuclease domain-containing protein
Locationchr3: 46449797 .. 46451084 (+)
RNA-Seq ExpressionLag0036435
SyntenyLag0036435
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTAGCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGGTGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCAACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCCACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTGTTAAGAACAAGGACTTTGCTTGCCCCGTGACAGTTCTAATCTTTGACATTGAAACGACAGGTCTTAGTAGAGACAAAGATAGAATAGTGGAAATCGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTACAAATTCATATCTTCATGACATTACAAACCATATGGTCAGTCGACCCGAAGTTCCGAGGTATTGAAATGCTCGGTGTAGTCGCATTCCTCTATTATTTACCCTCTCAGTTCAGTGCTAGTTAAATATATTGACTCCAATTTGAATCTTGGGTTAGGTTTATGCTAGGTTCCCCCATTCAGTTTTTCTAAGTAATTGACATTCATTTTTATCTACTGTGATATTTGCAGAATGGATCAATCAATATCTAAACGTTTCTTTTCTATGTTCAAGTAGGATGAAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTCATGTATTATTCATAGCTCATAATGGTCGTACCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTCAAAATTGGCTATTTTTCGATACCATGCATTTAGCCCGTCTGTTGATGAAATCCTCAGGTTAGTTTTTTTCCCTTTGCTCATATTTTCTTGCTGGTTTGTTTTGTGATAGAAAGATTAATGTGATGTAATGTAATATCGTGATCAGATTCGAAGATTACGAAAATCTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGTGCAATGTCGGACGTGACGTCGTTGTCGCGGATTCTTCAAAGGCTTACTTTCGACCTCAAGCTAGGCATCTCGTATCTGGTCGAAAGGTCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAG

mRNA sequence

ATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTAGCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGGTGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCAACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCCACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTGTTAAGAACAAGGACTTTGCTTGCCCCGTGACAGTTCTAATCTTTGACATTGAAACGACAGGTCTTAGTAGAGACAAAGATAGAATAGTGGAAATCGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTACAAATTCATATCTTCATGACATTACAAACCATATGGTCAGTCGACCCGAAGTTCCGAGGATGAAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTCATGTATTATTCATAGCTCATAATGGTCGTACCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTCAAAATTGGCTATTTTTCGATACCATGCATTTAGCCCGTCTGTTGATGAAATCCTCAGATTCGAAGATTACGAAAATCTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGTGCAATGTCGGACGTGACGTCGTTGTCGCGGATTCTTCAAAGGCTTACTTTCGACCTCAAGCTAGGCATCTCGTATCTGGTCGAAAGGTCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAG

Coding sequence (CDS)

ATGTGCTTTTCGATCTTGCGAGTTCCGAAGTTTGGAGTCCACACTTTAGCTAATTTATGGAGGGAAAACTTCCATCCCTTGAACAGGAATTATGGTCATAGTTCTTGGTATGGTCAGCTTGGTTTTAGAATCTATAACCTTGAAGGTGGGCAGAACAAGAGATGGACCAGAAAATCTGTGACCACAAAAGCAGGGAGGAAAGCCAAAACCAACCCCAGCCGTAAACCAAGCGATATCAGAAATGAAATCTTAGAGGAATCGGTCTCCACAAGCTGTACAGTTAGCATTAACAAGACTGAGAAAAGTGAAACTCAGAAGCTTCAATACTGTGATATTCAACAAACGATTGTTAAGAACAAGGACTTTGCTTGCCCCGTGACAGTTCTAATCTTTGACATTGAAACGACAGGTCTTAGTAGAGACAAAGATAGAATAGTGGAAATCGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTCCAAACACTAATAAATCCAGAGTGCTATATTACAAATTCATATCTTCATGACATTACAAACCATATGGTCAGTCGACCCGAAGTTCCGAGGATGAAGGAACTTATTCCAATTTTATTACAATTTGTTAAAAGCCGTCAGAAACCTGATGGTCATGTATTATTCATAGCTCATAATGGTCGTACCTTCGACGTGCCGTTTTTGCTCAAGGAATTCAGCCGTTGCTCCGTCGACATTCCTCAAAATTGGCTATTTTTCGATACCATGCATTTAGCCCGTCTGTTGATGAAATCCTCAGATTCGAAGATTACGAAAATCTCATTACAAGCTCTTCGCGAGTATTACGATATTCCGCTGGTGGGTTCAGCTCACCGTGCAATGTCGGACGTGACGTCGTTGTCGCGGATTCTTCAAAGGCTTACTTTCGACCTCAAGCTAGGCATCTCGTATCTGGTCGAAAGGTCTTTCACAGCTTCAGATCTGATTAATAAGAAGAAGAACTAG

Protein sequence

MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSVTTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNKDFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDITNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIPQNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRLTFDLKLGISYLVERSFTASDLINKKKN
Homology
BLAST of Lag0036435 vs. NCBI nr
Match: KAG6590475.1 (Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024006.1 Exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.0 bits (1421), Expect = 3.5e-153
Identity = 276/327 (84.40%), Postives = 301/327 (92.05%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFRIYNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +TKAGRKAKT+PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+TIVK+K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQF+KSRQKPDG+VL +AHN RTFDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKITKISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKKN 328
           TFDLKLGI  L+ERSFT SDLINKKKN
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lag0036435 vs. NCBI nr
Match: XP_038878496.1 (exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038878497.1 exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 550.1 bits (1416), Expect = 1.3e-152
Identity = 279/329 (84.80%), Postives = 298/329 (90.58%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILR PKFGV TLANLWRENFHPLNRN  HSSW+ QLGFRIYNLEGGQNKRW RKS+
Sbjct: 6   MCFSILRFPKFGVPTLANLWRENFHPLNRNCYHSSWHDQLGFRIYNLEGGQNKRWIRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAGRKAK NPS KP++IRNEILEES+STSCTVSINK+EKSETQKLQYCDIQQ I+KNK
Sbjct: 66  TTKAGRKAKANPSSKPTNIRNEILEESISTSCTVSINKSEKSETQKLQYCDIQQLIIKNK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI NS +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRNLDRIVEIALQDLSGGENSTFQTLINPECYIPNSVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSR--QKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVD 240
           NHMVSRP+VPRMKELIPILLQFV+SR  QKPDG+VL +AHN RTFDVPFLLKEFSRCS D
Sbjct: 186 NHMVSRPDVPRMKELIPILLQFVESRQKQKPDGYVLLVAHNARTFDVPFLLKEFSRCSFD 245

Query: 241 IPQNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           IP NW FFD++ LAR LMKSSDSKITKISLQALREYYDI LVGSAHRAMSDV SLS ILQ
Sbjct: 246 IPPNWRFFDSIPLARQLMKSSDSKITKISLQALREYYDIQLVGSAHRAMSDVRSLSLILQ 305

Query: 301 RLTFDLKLGISYLVERSFTASDLINKKKN 328
           RLTF+LKLGIS L+ERSFTASDLINKKKN
Sbjct: 306 RLTFELKLGISDLIERSFTASDLINKKKN 334

BLAST of Lag0036435 vs. NCBI nr
Match: XP_022961373.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 550.1 bits (1416), Expect = 1.3e-152
Identity = 275/327 (84.10%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFR YNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +TKAGRKAKT+PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+TIVK+K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQF+KSRQKPDG+VL +AHN RTFDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKITKISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKKN 328
           TFDLKLGI  L+ERSFT SDLINKKKN
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lag0036435 vs. NCBI nr
Match: XP_022968752.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 276/326 (84.66%), Postives = 298/326 (91.41%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFRIYNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAGRKAKTNPS KPS+IRNEILEESVSTSCTVSINK+EK  TQKLQ C+IQ+TI+K+K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQFVKSRQKPDG+VL +AHN R FDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKITKISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKK 327
           TFDLKLGI  L+ERSFT SDLINKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Lag0036435 vs. NCBI nr
Match: XP_023515555.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 274/327 (83.79%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFRIYNLEGGQNKRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQNKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +TKAGRKAKT+PS KPS+IRNEILEESVSTSCT+SINK+EK+ TQKLQ C+IQ+TI K+K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTISINKSEKNGTQKLQCCNIQETITKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQF+KSRQKPDG+VL +AHN RTFDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKI KISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKIKKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKKN 328
           TFDLKLGI  L+ERSFT SDLINKKKN
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lag0036435 vs. ExPASy Swiss-Prot
Match: Q682U6 (Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DPD1 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 143/323 (44.27%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  +++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECY-ITNSYLHDI 180
           D +  +TV++ D+ETTGL R  +RI+EIA QD++GG  STFQTL+NP    ITN+++H I
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDI 240
            N MV RPEVPRM+ELIPI L++V+SRQKP G+V+ +AHNG++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQNWLFFDTMHLARLLMKSSDSKI-TKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           P NWL  D++ LAR  MKS +  +    SL+AL +YY +   G AHRA+SDV  LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLGISYLVERSFTASDL 322
           +LT DLKL +S LV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lag0036435 vs. ExPASy Swiss-Prot
Match: Q8RA32 (DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=polC PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-10
Identity = 55/189 (29.10%), Postives = 96/189 (50.79%), Query Frame = 0

Query: 129 LIFDIETTGLSRDKDRIVEIAFQDLSGGE-NSTFQTLINPECYITN--SYLHDITNHMVS 188
           ++FDIETTGLS   D I+EI    +   +   TF+T +NP+  I++  + L  I   MV 
Sbjct: 389 VVFDIETTGLSNINDEIIEIGAVKIKNKKIVDTFETFVNPQIPISSFITKLTGIDESMVK 448

Query: 189 RPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIPQNWLF 248
             + P ++E++P  L+F K         + +AHN   FDV F+  +  +  + +    L 
Sbjct: 449 --DAPLIEEVLPKFLEFAKG-------AVLVAHNA-NFDVSFIKSKAKKLGLTVENTVL- 508

Query: 249 FDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRLTFDL- 308
            DT+ L+R L +     +    L  L E++++ L+   HRA+ D  + + I  ++   L 
Sbjct: 509 -DTLELSRHLYQD----LKNYKLDTLAEFFEVKLL-HHHRAVEDAKATAEIFIKMLEKLQ 560

Query: 309 KLGISYLVE 314
           ++GI  + E
Sbjct: 569 EIGIKSVSE 560

BLAST of Lag0036435 vs. ExPASy Swiss-Prot
Match: Q895K2 (DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=polC PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-10
Identity = 53/198 (26.77%), Postives = 92/198 (46.46%), Query Frame = 0

Query: 129 LIFDIETTGLSRDKDRIVEIAFQDLSGGE-NSTFQTLINPECYITNSYLHDITNHMVSRP 188
           ++FDIETTG S   D+I+EI    +  G+    + T +NPE  I    +     H     
Sbjct: 414 VVFDIETTGFSNKNDKIIEIGAVKIKEGKVVDKYSTFVNPERIIPEKIIELTGIHDYMVK 473

Query: 189 EVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIPQNWLFFD 248
           + P+++E++P  + F++         + +AHN   FDV F+ K      +    N    D
Sbjct: 474 DAPKIEEVLPKFIDFIEDS-------ILVAHNA-NFDVSFIKKNCKDFGIHF--NNPILD 533

Query: 249 TMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRLTFDLK-- 308
           T+ L + L      ++ +  L  + ++  IPL+ + HRA+ D  +   IL +   DLK  
Sbjct: 534 TIPLCKFLY----PELKRYKLNVVAKHLGIPLL-NHHRAVEDAKTTGDILLKAFEDLKDK 593

Query: 309 --LGISYLVERSFTASDL 322
             + ++ L E  F   D+
Sbjct: 594 EIMNLNMLNEEYFKNQDI 596

BLAST of Lag0036435 vs. ExPASy Swiss-Prot
Match: Q8XJR3 (DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=polC PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.3e-09
Identity = 54/173 (31.21%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 129 LIFDIETTGLSRDKDRIVEIAFQDLSGGE-NSTFQTLINPECYITN--SYLHDITNHMVS 188
           ++FDIETTG S   D+I+EI    L  GE   +F T ++P+  I    + L  IT +MV+
Sbjct: 436 VVFDIETTGFSSKNDKIIEIGAVKLKDGEIVDSFSTFVDPKVNIPYKITELTSITQNMVN 495

Query: 189 RPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIPQNWLF 248
               P + E++P  ++FV       G+ + +AHN   FDV F+ K        +      
Sbjct: 496 GQ--PTIDEVLPKFMEFV-------GNSVLVAHNA-AFDVGFIKKNLMDMGKTLKNP--V 555

Query: 249 FDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 299
            DT+ LAR L       + K+ L  + ++  I L  + HRA+ D  + + IL+
Sbjct: 556 MDTVPLARYLYPD----LKKVKLNLVAKHLGISL-ENHHRAVDDAKATAEILK 591

BLAST of Lag0036435 vs. ExPASy Swiss-Prot
Match: Q8EQU6 (DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=polC PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-09
Identity = 55/177 (31.07%), Postives = 89/177 (50.28%), Query Frame = 0

Query: 127 TVLIFDIETTGLSRDKDRIVEIAFQDLSGGE-NSTFQTLINPECYI--TNSYLHDITNHM 186
           T ++FD+ETTGLS   D I+E+A   + GGE    F++  NP   +  T   L  IT+ M
Sbjct: 413 TFVVFDVETTGLSAVYDTIIELAAVKIKGGEIVDRFESFANPHHALSQTTIDLTGITDDM 472

Query: 187 VSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIPQNW 246
           V+  + P + +++       K      G+ + +AHN  +FD+ FL + F R   +   N 
Sbjct: 473 VN--DAPEVADVL-------KDFHTWMGNDILVAHNA-SFDMGFLNQGFQRIDYEKASNP 532

Query: 247 LFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 301
           +  DT+ LAR L+     ++    L  L ++ DI L    HRA+ D  +   +L +L
Sbjct: 533 V-VDTLELARFLL----PELRNHRLNTLCKHLDIELT-QHHRAIYDAEATGYLLWKL 573

BLAST of Lag0036435 vs. ExPASy TrEMBL
Match: A0A6J1HDU6 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461962 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.4e-153
Identity = 275/327 (84.10%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFR YNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +TKAGRKAKT+PS KPS+IRNEILEESVSTSCTVSINK+EK+ TQKLQ C+IQ+TIVK+K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQF+KSRQKPDG+VL +AHN RTFDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKITKISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKKN 328
           TFDLKLGI  L+ERSFT SDLINKKKN
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKN 332

BLAST of Lag0036435 vs. ExPASy TrEMBL
Match: A0A6J1HUD7 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467897 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.4e-153
Identity = 276/326 (84.66%), Postives = 298/326 (91.41%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGV TLANLWRENFHPLNRNYGHSSWY QLGFRIYNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAGRKAKTNPS KPS+IRNEILEESVSTSCTVSINK+EK  TQKLQ C+IQ+TI+K+K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           + A PVTV+ FDIETTG SR+ DRIVEIA QDLSGGENSTFQTLINPECYI N+ +H IT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMKELIPILLQFVKSRQKPDG+VL +AHN R FDVPFLLKEFSRCSVDIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFD + LAR LMKS+DSKITKISLQALREYY+IPLVGSAHRAMSDV +LS ILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKK 327
           TFDLKLGI  L+ERSFT SDLINKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of Lag0036435 vs. ExPASy TrEMBL
Match: A0A6J1C1V8 (exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007515 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.0e-151
Identity = 273/327 (83.49%), Postives = 288/327 (88.07%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MCFSILRVPKFGVHTLAN WR NFHPLNRNYGHSSWY QL FRIYNLEGGQNKRWTRKS+
Sbjct: 6   MCFSILRVPKFGVHTLANFWRTNFHPLNRNYGHSSWYDQLCFRIYNLEGGQNKRWTRKSI 65

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAGRK K NPS KPSDIRNEILE SVSTSCTVSINKTEKSETQKLQYCDIQQT  K K
Sbjct: 66  TTKAGRKVKNNPSSKPSDIRNEILEGSVSTSCTVSINKTEKSETQKLQYCDIQQTFTKKK 125

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           +   PVTV+ FDIETTG SR KDRIVEIAFQDLSGGENSTFQTLINPECYI NS +H IT
Sbjct: 126 ELTRPVTVIAFDIETTGFSRKKDRIVEIAFQDLSGGENSTFQTLINPECYIPNSLVHGIT 185

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           + MVSRP+VPRMK+LIPILLQFV SRQKPDG++L +AHN RTFDVPFLL EFSRCS DIP
Sbjct: 186 SRMVSRPDVPRMKDLIPILLQFVLSRQKPDGYILLVAHNARTFDVPFLLNEFSRCSFDIP 245

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
           Q+WLF D M LAR LM SSDSK++KISLQALREYY IPLVGSAHRAMSDV SLS ILQRL
Sbjct: 246 QDWLFLDNMILARQLMISSDSKLSKISLQALREYYSIPLVGSAHRAMSDVMSLSLILQRL 305

Query: 301 TFDLKLGISYLVERSFTASDLINKKKN 328
           TFDLKL I  L+ERSFTASDLI K K+
Sbjct: 306 TFDLKLEIPDLIERSFTASDLIKKNKD 332

BLAST of Lag0036435 vs. ExPASy TrEMBL
Match: A0A0A0LVY3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 255/326 (78.22%), Postives = 281/326 (86.20%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI R PK+GV  LANLWRENFHPLNRN+GH+ WY QL FRIYNLEGG+NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAG KAKTNPS KP++IRNEIL+ES+  SCTV+INK+EKSETQKLQYCDIQ TI+K+K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           +FA  VTV+ FDIETTG SR+ DRIVEIAFQDLSGGENSTFQTLINP+CYITNS +H I+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           N MV  P VPRM+ELIPI+LQFVKSRQKP G+VL +AHN RTFDVPFLL EFSR SVDIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFDTM LAR LMK SDSKITKI+LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLGISYLVERSFTASDLINKKK 327
           TFDLKL IS LVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Lag0036435 vs. ExPASy TrEMBL
Match: A0A0N9LRH7 (Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.4e-139
Identity = 255/326 (78.22%), Postives = 280/326 (85.89%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI R PK+GV  LANLWRENFHPLNRN+GH+ WY QL FRIYNLEGG+NKRWTRKS+
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           TTKAG KAKTNPS KP++IRNEIL+ESV  SCTV+INK+EKSETQKLQYCDIQ TI+K+K
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECYITNSYLHDIT 180
           +FA  VTV+ FDIETTG SR+ DRIVEIAFQDLSGGENSTFQTLINP+CYITNS +H I+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDIP 240
           N MV  P VP M+ELIPI+LQFVKSRQKP G+VL +AHN RTFDVPFLL EFSR SVDIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QNWLFFDTMHLARLLMKSSDSKITKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQRL 300
            NWLFFDTM LAR LMK SDSKITKI+LQAL EYY I L G AHRA+SDV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLGISYLVERSFTASDLINKKK 327
           TFDLKL IS LVER+FT  DLINKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKK 326

BLAST of Lag0036435 vs. TAIR 10
Match: AT5G26940.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 7.7e-66
Identity = 143/323 (44.27%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  +++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECY-ITNSYLHDI 180
           D +  +TV++ D+ETTGL R  +RI+EIA QD++GG  STFQTL+NP    ITN+++H I
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDI 240
            N MV RPEVPRM+ELIPI L++V+SRQKP G+V+ +AHNG++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQNWLFFDTMHLARLLMKSSDSKI-TKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           P NWL  D++ LAR  MKS +  +    SL+AL +YY +   G AHRA+SDV  LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLGISYLVERSFTASDL 322
           +LT DLKL +S LV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lag0036435 vs. TAIR 10
Match: AT5G26940.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 7.7e-66
Identity = 143/323 (44.27%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  +++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECY-ITNSYLHDI 180
           D +  +TV++ D+ETTGL R  +RI+EIA QD++GG  STFQTL+NP    ITN+++H I
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDI 240
            N MV RPEVPRM+ELIPI L++V+SRQKP G+V+ +AHNG++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQNWLFFDTMHLARLLMKSSDSKI-TKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           P NWL  D++ LAR  MKS +  +    SL+AL +YY +   G AHRA+SDV  LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLGISYLVERSFTASDL 322
           +LT DLKL +S LV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lag0036435 vs. TAIR 10
Match: AT5G26940.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 7.7e-66
Identity = 143/323 (44.27%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  +++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECY-ITNSYLHDI 180
           D +  +TV++ D+ETTGL R  +RI+EIA QD++GG  STFQTL+NP    ITN+++H I
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDI 240
            N MV RPEVPRM+ELIPI L++V+SRQKP G+V+ +AHNG++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQNWLFFDTMHLARLLMKSSDSKI-TKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           P NWL  D++ LAR  MKS +  +    SL+AL +YY +   G AHRA+SDV  LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLGISYLVERSFTASDL 322
           +LT DLKL +S LV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of Lag0036435 vs. TAIR 10
Match: AT5G26940.4 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 7.7e-66
Identity = 143/323 (44.27%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 1   MCFSILRVPKFGVHTLANLWRENFHPLNRNYGHSSWYGQLGFRIYNLEGGQNKRWTRKSV 60
           MC SI +V +  +H+  +   E  H   +N   SS    L  R  +++G    RW R++V
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGRKAKTNPSRKPSDIRNEILEESVSTSCTVSINKTEKSETQKLQYCDIQQTIVKNK 120
           +T       T  SR  S+ ++ +L  +V  +  +     E+S T+   + ++QQ + ++K
Sbjct: 61  ST------TTQGSR--SNTKSSVLGGTVPVTRIID----EESRTKVQPFGNLQQRLAQDK 120

Query: 121 DFACPVTVLIFDIETTGLSRDKDRIVEIAFQDLSGGENSTFQTLINPECY-ITNSYLHDI 180
           D +  +TV++ D+ETTGL R  +RI+EIA QD++GG  STFQTL+NP    ITN+++H I
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNHMVSRPEVPRMKELIPILLQFVKSRQKPDGHVLFIAHNGRTFDVPFLLKEFSRCSVDI 240
            N MV RPEVPRM+ELIPI L++V+SRQKP G+V+ +AHNG++FD  FL+ EF+RCS +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQNWLFFDTMHLARLLMKSSDSKI-TKISLQALREYYDIPLVGSAHRAMSDVTSLSRILQ 300
           P NWL  D++ LAR  MKS +  +    SL+AL +YY +   G AHRA+SDV  LS++ Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLGISYLVERSFTASDL 322
           +LT DLKL +S LV R  TASD+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6590475.13.5e-15384.40Exonuclease DPD1, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma s... [more]
XP_038878496.11.3e-15284.80exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP... [more]
XP_022961373.11.3e-15284.10exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita moschata] >XP_022961374... [more]
XP_022968752.11.7e-15284.66exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1... [more]
XP_023515555.11.7e-15283.79exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q682U61.1e-6444.27Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8RA321.2e-1029.10DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongens... [more]
Q895K21.5e-1026.77DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) ... [more]
Q8XJR31.3e-0931.21DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=... [more]
Q8EQU65.0e-0931.07DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP... [more]
Match NameE-valueIdentityDescription
A0A6J1HDU66.4e-15384.10exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1HUD78.4e-15384.66exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1C1V86.0e-15183.49exonuclease DPD1, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0LVY31.1e-13978.22Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080... [more]
A0A0N9LRH72.4e-13978.22Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G26940.17.7e-6644.27Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.27.7e-6644.27Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.37.7e-6644.27Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.47.7e-6644.27Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 127..306
e-value: 3.2E-30
score: 116.4
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 129..293
e-value: 1.1E-19
score: 71.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 126..306
e-value: 3.2E-43
score: 149.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..77
NoneNo IPR availablePANTHERPTHR13058:SF23POLYMERASE III POLC-TYPE, PUTATIVE ISOFORM 1-RELATEDcoord: 1..324
NoneNo IPR availableCDDcd06127DEDDhcoord: 129..298
e-value: 6.56587E-39
score: 133.195
IPR040393Three-prime repair exonuclease 1/2PANTHERPTHR13058THREE PRIME REPAIR EXONUCLEASE 1, 2coord: 1..324
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 127..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036435.1Lag0036435.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000738 DNA catabolic process, exonucleolytic
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008408 3'-5' exonuclease activity