Lag0035579 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035579
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	rRNA N-glycosidase
Location	chr3: 24517929 .. 24518906 (-)
RNA-Seq Expression	Lag0035579
Synteny	Lag0035579

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCCTGGGGTCTGCTCAAGCTCAACCCTTGCCCTTGAATATTGCTGGCGCTCATCGTCGTGCCAACCCCAATCCTCGCGCTCATGCAGCTGCCCAACCTAATCTCCACTTCTCTCTGTTGGGTGCCACTGCCGAAACTTACAAACACTTCATAAACAAATTACGTCAGGCTCTCACACTGAAATCCCACAAGGTGTACAACATACCCGTGCTACCGCCACCAGCCACCGTCTCGGGGGCGGCTCGGTTCCTATTAATCGAGCTCACCAATTACAATCGTGAATCTATCATCATTGCAGTAGATGTAACTAACGTTTATGTGGTGGCATATCAAGCTAGAAACATAGCCTACTTTCTGAATGATGCTCCAAAAGAAGCAAGGAGTTTGCTATTTACAGGCATCAGGCGTGTAATACTTCCCTATAAGAGCACTTATGATACCCTCGAGAGAGATGCAGGACGCATTTCGAGAGAGAATATTGAATTGGGATTTCCTGCATTAAGTGGTTGTATTGGTAACATGTTCTACAATCAACAGGGAAATGCTATCCCCCGAGCATTTATTGTGATAGCTCAGATGATTCCTGAAGCGGCGAGATTTAAATACATTGAGCAAAGAGTTTCCGAGAGCGTTGAGGTACTATTTAAGCCAAAACTAGTATTCTTAACTTTAGAAAACAATTGGCAGAAGTTCTCAGAACAAATACAAATCGCACCGAAACGAGGAGGAAATTTTGCTACTCCCGTTGTGGTTCGAAGCGTTACTGACAAAGAAATTAAAGTAACCGATGTTAAGTCGCCAGTTGTTAAGGGTATTGCACTGTTACTTCACAGCAAGTCAGAAGCAAATGGACATCAAACCAAGCTGATGATGGCAGAGCTCAATGGATGGGAATTCGTCGCTGGTGGAGATAATGGTATGAAACTGATGGAAGAGGAAAATGTTGGAGGTGCCTTTGGAATTGAGTTTTGA

mRNA sequence

ATGGAGTGCCTGGGGTCTGCTCAAGCTCAACCCTTGCCCTTGAATATTGCTGGCGCTCATCGTCGTGCCAACCCCAATCCTCGCGCTCATGCAGCTGCCCAACCTAATCTCCACTTCTCTCTGTTGGGTGCCACTGCCGAAACTTACAAACACTTCATAAACAAATTACGTCAGGCTCTCACACTGAAATCCCACAAGGTGTACAACATACCCGTGCTACCGCCACCAGCCACCGTCTCGGGGGCGGCTCGGTTCCTATTAATCGAGCTCACCAATTACAATCGTGAATCTATCATCATTGCAGTAGATGTAACTAACGTTTATGTGGTGGCATATCAAGCTAGAAACATAGCCTACTTTCTGAATGATGCTCCAAAAGAAGCAAGGAGTTTGCTATTTACAGGCATCAGGCGTGTAATACTTCCCTATAAGAGCACTTATGATACCCTCGAGAGAGATGCAGGACGCATTTCGAGAGAGAATATTGAATTGGGATTTCCTGCATTAAGTGGTTGTATTGGTAACATGTTCTACAATCAACAGGGAAATGCTATCCCCCGAGCATTTATTGTGATAGCTCAGATGATTCCTGAAGCGGCGAGATTTAAATACATTGAGCAAAGAGTTTCCGAGAGCGTTGAGGTACTATTTAAGCCAAAACTAGTATTCTTAACTTTAGAAAACAATTGGCAGAAGTTCTCAGAACAAATACAAATCGCACCGAAACGAGGAGGAAATTTTGCTACTCCCGTTGTGGTTCGAAGCGTTACTGACAAAGAAATTAAAGTAACCGATGTTAAGTCGCCAGTTGTTAAGGGTATTGCACTGTTACTTCACAGCAAGTCAGAAGCAAATGGACATCAAACCAAGCTGATGATGGCAGAGCTCAATGGATGGGAATTCGTCGCTGGTGGAGATAATGGTATGAAACTGATGGAAGAGGAAAATGTTGGAGGTGCCTTTGGAATTGAGTTTTGA

Coding sequence (CDS)

ATGGAGTGCCTGGGGTCTGCTCAAGCTCAACCCTTGCCCTTGAATATTGCTGGCGCTCATCGTCGTGCCAACCCCAATCCTCGCGCTCATGCAGCTGCCCAACCTAATCTCCACTTCTCTCTGTTGGGTGCCACTGCCGAAACTTACAAACACTTCATAAACAAATTACGTCAGGCTCTCACACTGAAATCCCACAAGGTGTACAACATACCCGTGCTACCGCCACCAGCCACCGTCTCGGGGGCGGCTCGGTTCCTATTAATCGAGCTCACCAATTACAATCGTGAATCTATCATCATTGCAGTAGATGTAACTAACGTTTATGTGGTGGCATATCAAGCTAGAAACATAGCCTACTTTCTGAATGATGCTCCAAAAGAAGCAAGGAGTTTGCTATTTACAGGCATCAGGCGTGTAATACTTCCCTATAAGAGCACTTATGATACCCTCGAGAGAGATGCAGGACGCATTTCGAGAGAGAATATTGAATTGGGATTTCCTGCATTAAGTGGTTGTATTGGTAACATGTTCTACAATCAACAGGGAAATGCTATCCCCCGAGCATTTATTGTGATAGCTCAGATGATTCCTGAAGCGGCGAGATTTAAATACATTGAGCAAAGAGTTTCCGAGAGCGTTGAGGTACTATTTAAGCCAAAACTAGTATTCTTAACTTTAGAAAACAATTGGCAGAAGTTCTCAGAACAAATACAAATCGCACCGAAACGAGGAGGAAATTTTGCTACTCCCGTTGTGGTTCGAAGCGTTACTGACAAAGAAATTAAAGTAACCGATGTTAAGTCGCCAGTTGTTAAGGGTATTGCACTGTTACTTCACAGCAAGTCAGAAGCAAATGGACATCAAACCAAGCTGATGATGGCAGAGCTCAATGGATGGGAATTCGTCGCTGGTGGAGATAATGGTATGAAACTGATGGAAGAGGAAAATGTTGGAGGTGCCTTTGGAATTGAGTTTTGA

Protein sequence

MECLGSAQAQPLPLNIAGAHRRANPNPRAHAAAQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAELNGWEFVAGGDNGMKLMEEENVGGAFGIEF

Homology

[Close]

BLAST of Lag0035579 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 316.2 bits (809), Expect = 3.2e-82
Identity = 183/329 (55.62%), Postives = 212/329 (64.44%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FIN L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRAPRPSANA----NLSFSLLGATAQTYKQFINDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R ALT++SHKVY IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RNALTVQSHKVYGIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG ISRENIELGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+   G ++PR+FIV+ QM+ EA+RFKYIE R  E VE  FKP   +L+LEN W   SEQ
Sbjct: 181 FHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F  PV VRSV++K IKVTDV SPVVKGIALLL +K   NG     +M + 
Sbjct: 241 VQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNG--VSKLMEQF 300

Query: 301 NGWEFVAGGDNGMKLMEEENVGGAFGIEF 326
             WE                  GAFGI+F
Sbjct: 301 IHWEAAR---------------GAFGIDF 305

[Close]

BLAST of Lag0035579 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 314.3 bits (804), Expect = 1.2e-81
Identity = 176/308 (57.14%), Postives = 208/308 (67.53%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FIN L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRAPRPSANA----NLSFSLLGATAQTYKQFINDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY+IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYDIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG+ISRENIELGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+ + G ++PR+FIV+ QM+ EA+RFKYIE R  E VE  FKP   +L+LEN W   SEQ
Sbjct: 181 FHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F  PV VRSV++K + V+DV SPVVKGIALLL +K   NG     +M E 
Sbjct: 241 VQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNG--VSKLMEEF 299

Query: 301 NGWEFVAG 305
             WE   G
Sbjct: 301 IHWEAARG 299

[Close]

BLAST of Lag0035579 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.2 bits (801), Expect = 2.7e-81
Identity = 176/308 (57.14%), Postives = 207/308 (67.21%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FIN L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRAPRPSANA----NLSFSLLGATAQTYKQFINDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY+IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYDIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG+ISRENIELGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+   G ++PR+FIV+ QM+ EA+RFKYIE R  E VE  FKP   +L+LEN W   SEQ
Sbjct: 181 FHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F  PV VRSV++K + V+DV SPVVKGIALLL +K   NG     +M E 
Sbjct: 241 VQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNG--VSKLMEEF 299

Query: 301 NGWEFVAG 305
             WE   G
Sbjct: 301 IKWEAARG 299

[Close]

BLAST of Lag0035579 vs. NCBI nr
Match: KAG6607631.1 (hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.4 bits (799), Expect = 4.6e-81
Identity = 173/290 (59.66%), Postives = 201/290 (69.31%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FIN L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRAPRPSANA----NLSFSLLGATAQTYKQFINDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY+IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYDIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG+ISRENIELGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+   G ++PR+FIV+ QM+ EA+RFKYIE R  E VE  FKP   +L+LEN W   SE 
Sbjct: 181 FHYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEH 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANG 287
           +Q A K GG F  PV VRSV++K IKVTDV SPVVKGIALLL +K   NG
Sbjct: 241 VQKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNG 283

[Close]

BLAST of Lag0035579 vs. NCBI nr
Match: KAG7037234.1 (hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.5 bits (794), Expect = 1.8e-80
Identity = 178/313 (56.87%), Postives = 212/313 (67.73%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    N+ FSLLGATAETYK FI  L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRVPRPSANA----NVSFSLLGATAETYKQFIKDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY IPVL  PAT  G ARF+L+ LTNYN ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYGIPVL--PATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA ++LF G +   L YK  YD LER AG+ISREN++LGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+   G ++PR+FIV+ QM+ EA+RFKYIE+  S++V   FKP   +L+LEN W   SEQ
Sbjct: 181 FHYNAGYSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F   V VRSV +K IKVTDV SPVVKGIALLL +K  ANG     +M E 
Sbjct: 241 VQKAQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANG--VSKLMEEF 300

Query: 301 NGWEFVAGGDNGM 310
             WE  A GD G+
Sbjct: 301 IHWE-AARGDFGI 303

[Close]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 149/251 (59.36%), Postives = 179/251 (71.31%), Query Frame = 0


Query: 29  AHAAAQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLI 88
           AH     +++FSL+GAT  TYK FI  LR  LT+ + +VY+IPVL   A  +G ARF L+
Sbjct: 16  AHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA--AGLARFQLV 75

Query: 89  ELTNYNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYD 148
            LTNYN ES+ +A+DV NVYVVAY+A N AYFL DA  EA ++LF GI  V LPY   YD
Sbjct: 76  TLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYD 135

Query: 149 TLERDAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQR 208
            LE  AGRISRENIELGF  +S  IGNMF +  G ++PRAFIVI Q + EAARFKYIEQR
Sbjct: 136 GLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQR 195

Query: 209 VSESVEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKS 268
           VSE+V   FKP   FL+L+N W   SEQIQIA  RGG FA PV +R+V++    VT+V S
Sbjct: 196 VSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNS 255

Query: 269 PVVKGIALLLH 280
           PVVKGIALLL+
Sbjct: 256 PVVKGIALLLY 264

[Close]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 5.0e-54
Identity = 118/251 (47.01%), Postives = 168/251 (66.93%), Query Frame = 0


Query: 33  AQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTN 92
           A+ +++F L  ATA+TY  FI   R  L   SHKVY+IP+L   +T+S + RF+L++LT+
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLL--YSTISDSRRFILLDLTS 80

Query: 93  YNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLER 152
           Y  E+I +A+DVTNVYVVAY+ R+++YF  ++P EA ++LF G R++ LPY   Y+ L+ 
Sbjct: 81  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 140

Query: 153 DAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSES 212
            A +I RENI+LG PALS  I  +FY    +A P A +V+ Q   EAARFKYIE+ V++ 
Sbjct: 141 AAHKI-RENIDLGLPALSSAITTLFYYNAQSA-PSALLVLIQTTAEAARFKYIERHVAKY 200

Query: 213 VEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVK 272
           V   FKP L  ++LEN W   S+QI +A  +GG F  PV +   T +  +VT+V S VVK
Sbjct: 201 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 260

Query: 273 G-IALLLHSKS 283
           G I LLL+S++
Sbjct: 261 GNIKLLLNSRA 266

[Close]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 8.6e-54
Identity = 118/251 (47.01%), Postives = 167/251 (66.53%), Query Frame = 0


Query: 33  AQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTN 92
           A+ +++F L  ATA+TY  FI   R  L   SHKVY+IP+L   +T+S + RF+L+ LT+
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLL--YSTISDSRRFILLNLTS 80

Query: 93  YNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLER 152
           Y  E+I +A+DVTNVYVVAY+ R+++YF  ++P EA ++LF G R++ LPY   Y+ L+ 
Sbjct: 81  YAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQT 140

Query: 153 DAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSES 212
            A +I RENI+LG PALS  I  +FY    +A P A +V+ Q   EAARFKYIE+ V++ 
Sbjct: 141 AAHKI-RENIDLGLPALSSAITTLFYYNAQSA-PSALLVLIQTTAEAARFKYIERHVAKY 200

Query: 213 VEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVK 272
           V   FKP L  ++LEN W   S+QI +A  +GG F  PV +   T +  +VT+V S VVK
Sbjct: 201 VATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVK 260

Query: 273 G-IALLLHSKS 283
           G I LLL+S++
Sbjct: 261 GNIKLLLNSRA 266

[Close]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 7.0e-48
Identity = 108/245 (44.08%), Postives = 151/245 (61.63%), Query Frame = 0


Query: 36  NLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNR 95
           N+ F L  AT+ +YK FI  LR+AL  K  KVY+IPVL   +TV  + RF+LI+L NY+ 
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALP-KDGKVYDIPVL--LSTVMDSRRFILIDLVNYDG 60

Query: 96  ESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAG 155
           +SI  A+DV NVY+VAY    ++YF    P +A  LLF G ++  LPY   Y+ L+  A 
Sbjct: 61  QSITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAK 120

Query: 156 RISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEV 215
           ++ RENIELG PAL   I  +F+     A   A +V+ Q   EAARF+YIE +++ +V  
Sbjct: 121 KL-RENIELGLPALDSAITTLFH-YNAEAAASALLVLIQTTSEAARFRYIELQIANNVGT 180

Query: 216 LFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVV-KGI 275
            FKP    ++LENNW   S+QIQIA  + G F TPV++       +++T+V S VV + I
Sbjct: 181 KFKPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNI 240

Query: 276 ALLLH 280
            LLL+
Sbjct: 241 QLLLN 240

[Close]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.6e-46
Identity = 110/256 (42.97%), Postives = 162/256 (63.28%), Query Frame = 0


Query: 32  AAQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELT 91
           A + ++ F L GAT+ +Y  FI+ LR+AL  +  K+Y+IP+L   +T+ G+ R+ LI LT
Sbjct: 20  AVEGDVSFRLSGATSSSYGVFISNLRKALPYE-RKLYDIPLL--RSTLPGSQRYALIHLT 79

Query: 92  NYNRESIIIAVDVTNVYVVAYQARNIAYFLNDA-PKEARSLLFTGI-RRVILPYKSTYDT 151
           NY  E+I +A+DVTNVYV+ Y+A + +YF N+A   EA   +F    R+V LPY   Y+ 
Sbjct: 80  NYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAKRKVTLPYSGNYER 139

Query: 152 LERDAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRV 211
           L+  AG+I RENI LG PAL   I  +FY    N+   A +V+ Q   EAAR+K+IEQ++
Sbjct: 140 LQIAAGKI-RENIPLGLPALDSAITTLFY-YNANSAASALMVLIQSTSEAARYKFIEQQI 199

Query: 212 SESVEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSP 271
            + V+  F P L  ++LEN+W   S+QIQIA    G F TPVV+ +  ++ + +T+V + 
Sbjct: 200 GKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQRVTITNVDAG 259

Query: 272 VV-KGIALLLHSKSEA 285
           VV   IALLL+  + A
Sbjct: 260 VVTSNIALLLNRNNMA 270

[Close]

BLAST of Lag0035579 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 5.9e-82
Identity = 176/308 (57.14%), Postives = 208/308 (67.53%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS Q     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FIN L
Sbjct: 1   MECIGSTQDLIQAPFPLN-CGAHAAIRAPRPSANA----NLSFSLLGATAQTYKQFINDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY+IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYDIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG+ISRENIELGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+ + G ++PR+FIV+ QM+ EA+RFKYIE R  E VE  FKP   +L+LEN W   SEQ
Sbjct: 181 FHYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F  PV VRSV++K + V+DV SPVVKGIALLL +K   NG     +M E 
Sbjct: 241 VQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNG--VSKLMEEF 299

Query: 301 NGWEFVAG 305
             WE   G
Sbjct: 301 IHWEAARG 299

[Close]

BLAST of Lag0035579 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.2e-80
Identity = 179/328 (54.57%), Postives = 216/328 (65.85%), Query Frame = 0


Query: 1   MECLGSAQ--AQPLPLNIAGAHR-RANPNPRAHAAAQPNLHFSLLGATAETYKHFINKLR 60
           MEC+ S++   QPLP N  G H   AN    A  A   N+ FSLLGAT +TY+ FI  LR
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHAN---GAVNAPNSNVSFSLLGATGKTYQQFIQNLR 60

Query: 61  QALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARNI 120
            ALT+ S  VYNIPVL   AT   +ARF+L+ LTNY  E+I +A+DV NVY+VAYQA N 
Sbjct: 61  NALTINSKIVYNIPVL--AATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNK 120

Query: 121 AYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNMF 180
           AYFL DA KEAR +LF GI++ ILPYK  YD LE  AG+ISRE I+LGF  L   IGNM+
Sbjct: 121 AYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMY 180

Query: 181 YNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQI 240
           +   G ++PRAFIV+ Q I EAARFKYIE +VS++VE  FKP   FL+LEN W   SEQ+
Sbjct: 181 HYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQV 240

Query: 241 QIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAELN 300
           QIA  R G FA P+ VRSVT+K I VT+VKS VV+G+ALLL+SK   NG   +L M E N
Sbjct: 241 QIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMEL-MEEFN 300

Query: 301 GWEFVAGGDNGMKLMEEENVGGAFGIEF 326
                          E EN  G FGI+F
Sbjct: 301 --------------KEWENGRGEFGIDF 308

[Close]

BLAST of Lag0035579 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 2.1e-79
Identity = 171/308 (55.52%), Postives = 208/308 (67.53%), Query Frame = 0


Query: 1   MECLGSAQ---AQPLPLNIAGAHRRAN-PNPRAHAAAQPNLHFSLLGATAETYKHFINKL 60
           MEC+GS +     P PLN  GAH     P P A+A    NL FSLLGATA+TYK FI+ L
Sbjct: 1   MECIGSTKDLIQAPFPLN-CGAHVAIRAPRPSANA----NLSFSLLGATAQTYKQFISDL 60

Query: 61  RQALTLKSHKVYNIPVLPPPATVSGAARFLLIELTNYNRESIIIAVDVTNVYVVAYQARN 120
           R+ALT+ SHKVY+IPVL  PAT  G ARF+L+ LTNY  ESI +A+D  +VY+VAYQA N
Sbjct: 61  RKALTVNSHKVYDIPVL--PATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGN 120

Query: 121 IAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLERDAGRISRENIELGFPALSGCIGNM 180
            AYFL D  KEA  +LF G +   L YK  YD LER AG+ISRENI+LGF  LS  IGNM
Sbjct: 121 KAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNM 180

Query: 181 FYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSESVEVLFKPKLVFLTLENNWQKFSEQ 240
           F+   G ++P++FIV+ QM+ EA+RFKYIE R  E+VE  FKP   +L+LEN W   SEQ
Sbjct: 181 FHYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQ 240

Query: 241 IQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVVKGIALLLHSKSEANGHQTKLMMAEL 300
           +Q A K GG F  PV VRSV++K + V+DV SPVVKG+ALLL +K   NG     +M E 
Sbjct: 241 VQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNG--VSKLMEEF 299

Query: 301 NGWEFVAG 305
             WE   G
Sbjct: 301 IKWEAARG 299

[Close]

BLAST of Lag0035579 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 9.2e-75
Identity = 155/250 (62.00%), Postives = 188/250 (75.20%), Query Frame = 0


Query: 32  AAQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELT 91
           AA+ ++ FS+LG+T+ETYK FI  +R ALT+ S  VY+IPVL   AT +G+ARFLL+ LT
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVL--KATATGSARFLLVHLT 77

Query: 92  NYNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLE 151
           NYN ESII+A+DV NVYVVAY+A N AYFLNDA  EA  +LF GI  V L YK  YD LE
Sbjct: 78  NYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLE 137

Query: 152 RDAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSE 211
             A +ISRE I+LGF  +S  IGNMF++  G ++PRAFIVI Q + EAARFKYIEQRVSE
Sbjct: 138 -TAAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSE 197

Query: 212 SVEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVV 271
           +V+  FKP   FL+L+N W   SEQIQIA KRGG FA  V +R+V++K I VTDV SPVV
Sbjct: 198 NVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVV 257

Query: 272 KGIALLLHSK 282
           KGIALLL+ K
Sbjct: 258 KGIALLLYYK 264

[Close]

BLAST of Lag0035579 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 4.5e-74
Identity = 150/250 (60.00%), Postives = 187/250 (74.80%), Query Frame = 0


Query: 32  AAQPNLHFSLLGATAETYKHFINKLRQALTLKSHKVYNIPVLPPPATVSGAARFLLIELT 91
           AA+ ++ FS+LGAT+ETY+ FI  LR  LT++S  VY IPVL   +T  G+ARF+L+ LT
Sbjct: 18  AAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVL--QSTAVGSARFILVHLT 77

Query: 92  NYNRESIIIAVDVTNVYVVAYQARNIAYFLNDAPKEARSLLFTGIRRVILPYKSTYDTLE 151
           NYN ESI +A+DV N+YVVAY+A N AYFLNDA  EA ++LF GI+ V LPYK  YD LE
Sbjct: 78  NYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGLE 137

Query: 152 RDAGRISRENIELGFPALSGCIGNMFYNQQGNAIPRAFIVIAQMIPEAARFKYIEQRVSE 211
             AG+ISRE I+LGF  +S  IGNMF++  G +I RAFIVI Q + EAARFKYIEQRVSE
Sbjct: 138 TAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSE 197

Query: 212 SVEVLFKPKLVFLTLENNWQKFSEQIQIAPKRGGNFATPVVVRSVTDKEIKVTDVKSPVV 271
           +V+  FKP   FL+LEN W   SEQIQIA KR G FA  + +R+V++K I VT+V SP+V
Sbjct: 198 NVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIV 257

Query: 272 KGIALLLHSK 282
           KGIALLL+ K
Sbjct: 258 KGIALLLYYK 265

The following BLAST results are available for this feature:

BLAST of Lag0035579 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523582.1	3.2e-82	55.62	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
XP_022926255.1	1.2e-81	57.14	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
KAG7037232.1	2.7e-81	57.14	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6607631.1	4.6e-81	59.66	hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7037234.1	1.8e-80	56.87	hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lag0035579 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.7e-73	59.36	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P29339	5.0e-54	47.01	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P24817	8.6e-54	47.01	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P84531	7.0e-48	44.08	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P24478	8.6e-46	42.97	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]

BLAST of Lag0035579 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKL0	5.9e-82	57.14	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1E093	3.2e-80	54.57	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	2.1e-79	55.52	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1DVE7	9.2e-75	62.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
A0A6J1DWK4	4.5e-74	60.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]

BLAST of Lag0035579 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 159..173 score: 48.77 coord: 189..209 score: 55.14 coord: 221..238 score: 41.37 coord: 97..112 score: 50.99 coord: 47..60 score: 40.23
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 201..283 e-value: 3.7E-22 score: 80.1
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 45..238 e-value: 3.6E-30 score: 105.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 36..288
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 31..200 e-value: 1.1E-41 score: 144.3
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 36..288
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 193..209
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 34..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035579.1	Lag0035579.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity