Lag0035551 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0035551 vs. NCBI nr
Match: XP_023531170.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.8 bits (1693), Expect = 1.4e-184
Identity = 333/402 (82.84%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 62  PFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQ 121
           P+P P PL  K        PPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQ
Sbjct: 49  PYPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQ 108

Query: 122 EGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRL 181
           EGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI+++DPDLIAAFDT GAELLYEIRL
Sbjct: 109 EGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGAELLYEIRL 168

Query: 182 LSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYR 241
           L+AEKRA+AA+YIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YR
Sbjct: 169 LNAEKRAAAARYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYR 228

Query: 242 LSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVR 301
           LSREY N  EART+ALEDALKV VTE ARSLIVRDLE EGEGK+ AEEE EAG+KVPVVR
Sbjct: 229 LSREYSNMSEARTSALEDALKVAVTEEARSLIVRDLEREGEGKDEAEEEIEAGVKVPVVR 288

Query: 302 LKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEP 361
           LKIGEVAEATTV+V+PVC  EEG KGVG APMEVRSEGEFGVVV EEGWRRWVVLP WEP
Sbjct: 289 LKIGEVAEATTVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVVEEGWRRWVVLPGWEP 348

Query: 362 LRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLT-AAGGEGE 421
           + GLG+GGVVVAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT A G +GE
Sbjct: 349 IAGLGKGGVVVAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGATGNDGE 408

Query: 422 LKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           LKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 409 LKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 450

BLAST of Lag0035551 vs. NCBI nr
Match: XP_022933155.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 656.4 bits (1692), Expect = 1.9e-184
Identity = 335/412 (81.31%), Postives = 360/412 (87.38%), Query Frame = 0

Query: 52  PSNPHLRHSQPFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFE 111
           P NP      P P P PL  K        PPPSPLP QYRSLD  GKLDVL+NRLGLWFE
Sbjct: 36  PPNPISASLNPSPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFE 95

Query: 112 YAPLISSLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTS 171
           YAPLISSLIQEGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI+++DPDLIAAFDT 
Sbjct: 96  YAPLISSLIQEGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTG 155

Query: 172 GAELLYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFP 231
           GAELLYEIRLL+AEKRA+AA+YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+P
Sbjct: 156 GAELLYEIRLLNAEKRAAAARYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYP 215

Query: 232 GDCLAFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEET 291
           GDCLAFM+YRLSREY N  EARTAALEDALKV VTE ARSLIVRDLE EGEGK+  EEE 
Sbjct: 216 GDCLAFMHYRLSREYSNMSEARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEVEEEI 275

Query: 292 EAGLKVPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWR 351
           EAG++VPVVRLKIGEVAEATTV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWR
Sbjct: 276 EAGVRVPVVRLKIGEVAEATTVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWR 335

Query: 352 RWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFY 411
           RWVVLP WEP+ GLG+GGVVVAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFY
Sbjct: 336 RWVVLPGWEPIAGLGKGGVVVAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFY 395

Query: 412 LT-AAGGEGELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           LT A G +GELKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 396 LTGATGSDGELKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lag0035551 vs. NCBI nr
Match: KAG6588314.1 (Rubisco accumulation factor 1.2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020875.1 Rubisco accumulation factor 1.2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653.7 bits (1685), Expect = 1.2e-183
Identity = 332/402 (82.59%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 62  PFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQ 121
           P P P PL  K        PPPSPLP QYRSLD  GKLDVL+NRLGLWFEYAPLISSLIQ
Sbjct: 46  PSPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFEYAPLISSLIQ 105

Query: 122 EGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRL 181
           EGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI+++DPDLIAAFDT G+ELLYEIRL
Sbjct: 106 EGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTGGSELLYEIRL 165

Query: 182 LSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYR 241
           L+AEKRA+AA+YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+PGDCLAFM+YR
Sbjct: 166 LNAEKRAAAARYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYPGDCLAFMHYR 225

Query: 242 LSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVR 301
           LSREY N  EARTAALEDALKV VTE ARSLIVRDLE EGEGK+ AEEE EAG++VPVVR
Sbjct: 226 LSREYSNMSEARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEAEEEIEAGVRVPVVR 285

Query: 302 LKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEP 361
           LKIGEVAEATTV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWRRWVVLP WEP
Sbjct: 286 LKIGEVAEATTVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWRRWVVLPGWEP 345

Query: 362 LRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLT-AAGGEGE 421
           + GLG+GGVVVAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFYLT A G +GE
Sbjct: 346 IAGLGKGGVVVAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFYLTGATGSDGE 405

Query: 422 LKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           LKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 406 LKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lag0035551 vs. NCBI nr
Match: XP_022965491.1 (rubisco accumulation factor 1.1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 650.2 bits (1676), Expect = 1.3e-182
Identity = 335/424 (79.01%), Postives = 363/424 (85.61%), Query Frame = 0

Query: 48  LPPPPSNPHLRHS--------QPFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKL 107
           L  PP    LRH          PFP P PL  K        PPPSPLP QYRSLD  GKL
Sbjct: 57  LHTPPLRLFLRHPPNLISASLNPFPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKL 116

Query: 108 DVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDN 167
           DVL+NRLGLWFEYAPLISSLIQEGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI++
Sbjct: 117 DVLANRLGLWFEYAPLISSLIQEGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTIND 176

Query: 168 SDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRG 227
           +DPDLIAAFDT GAELLYEIRLL+AEKRA+AA+YI++NRLDGKGAQDLARAMKDFPRRRG
Sbjct: 177 TDPDLIAAFDTGGAELLYEIRLLNAEKRAAAARYIMENRLDGKGAQDLARAMKDFPRRRG 236

Query: 228 DKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEG 287
           DKGWESF+YD+PGDCLAFM+YRLSREY N  EARTAAL DALKV VTE ARSLIVRDLE 
Sbjct: 237 DKGWESFNYDYPGDCLAFMHYRLSREYSNMSEARTAALADALKVAVTEEARSLIVRDLER 296

Query: 288 EGEGKEGAEEETEAGLKVPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEG 347
           EGEGK+GAEEE EAG++VPVVRLKIGEVAEATTV+V+PVC  EEG KGV  APMEVRSEG
Sbjct: 297 EGEGKDGAEEEIEAGVRVPVVRLKIGEVAEATTVVVLPVCNGEEGGKGVREAPMEVRSEG 356

Query: 348 EFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDR 407
           EFGVVVAEEGWRRWVVLP WEP+ GLG+GGVVV F DAR+LPWRVNRWYKEEAILVV DR
Sbjct: 357 EFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVVVVFKDARSLPWRVNRWYKEEAILVVADR 416

Query: 408 SRNEVVAEDGFYLT-AAGGEGELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSD 463
           SR EV A DGFY+T A G +GELKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSD
Sbjct: 417 SRKEVEAGDGFYMTGATGNDGELKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSD 476

BLAST of Lag0035551 vs. NCBI nr
Match: XP_038880540.1 (rubisco accumulation factor 1.1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 624.8 bits (1610), Expect = 6.0e-175
Identity = 322/399 (80.70%), Postives = 352/399 (88.22%), Query Frame = 0

Query: 66  PFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFT 125
           P PL+ K        PPPSPLP QYRSLD  GKL+VLSNRLGLWFEYAPLISSLIQEGFT
Sbjct: 50  PSPLAAKQQVYQPFRPPPSPLPSQYRSLDAEGKLNVLSNRLGLWFEYAPLISSLIQEGFT 109

Query: 126 PPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAE 185
           PPT+EEITGI+GVQQNI +VGAQVR+SLV + +N+DPD+IAAFDTSGAELLYEIRLLS E
Sbjct: 110 PPTLEEITGITGVQQNIFVVGAQVRESLVQS-NNADPDVIAAFDTSGAELLYEIRLLSTE 169

Query: 186 KRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSRE 245
           KRA+AA+YIV+NRLD KGAQDLARA+KDFPRRRGDKGWE FDY+ PGDCLA+MYYRLSRE
Sbjct: 170 KRAAAARYIVENRLDIKGAQDLARALKDFPRRRGDKGWECFDYEEPGDCLAYMYYRLSRE 229

Query: 246 YKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLKIG 305
           Y N  E RTAALE+ALKVVVTE AR LIV DLEG+G+GKEG EEE E G++VPVVR+KIG
Sbjct: 230 YNNPSEPRTAALEEALKVVVTEKARDLIVGDLEGKGDGKEGVEEEIEVGIRVPVVRMKIG 289

Query: 306 EVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGL 365
           EVAEATTV+VMPVCKA EGEKGVG APME+RSEGEFGVVVAE+ WR+WVVLP WEP+ GL
Sbjct: 290 EVAEATTVLVMPVCKAGEGEKGVGEAPMELRSEGEFGVVVAEKEWRKWVVLPGWEPVVGL 349

Query: 366 GR-GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAG-GEGELKV 425
            + GGVVVAF DARALPWRVNRWYKEE ILVV DRSR EVVA DGFYLT A  G GELKV
Sbjct: 350 VKGGGVVVAFEDARALPWRVNRWYKEEPILVVVDRSRKEVVAGDGFYLTGATVGGGELKV 409

Query: 426 QRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           QRGNALME GV+ESLGTVLLVVRPPK+MEDDQLSDEDWD
Sbjct: 410 QRGNALMEMGVKESLGTVLLVVRPPKEMEDDQLSDEDWD 447

BLAST of Lag0035551 vs. ExPASy Swiss-Prot
Match: Q9LKR8 (Rubisco accumulation factor 1.1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.1 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 5.7e-120
Identity = 231/384 (60.16%), Postives = 292/384 (76.04%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+TGISGV+Q
Sbjct: 60  PPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISGVEQ 119

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N LIVGAQVR SLV +   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+YIVD+  D
Sbjct: 120 NSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHGFD 179

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
            KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E RT  LE A
Sbjct: 180 TKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLEQA 239

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLKIGEVAEATTVMVMPVCK 320
           L+  VTE A+  ++R+L GE E +   EEE +  ++VPVVRL+ GEVA A++V+V+PVCK
Sbjct: 240 LETAVTEKAKKAVLRELHGESEEERVKEEEIKI-IRVPVVRLRFGEVAGASSVVVLPVCK 299

Query: 321 AEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDAR-A 380
           AEEGE+ +  APME  S GEFGVV AE+ W RWVVLP W+P+  + R GV V+F+D R  
Sbjct: 300 AEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAV-RKGVAVSFSDDREV 359

Query: 381 LPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVRESLG 440
           LPW      K EAI+VV DR +  V A++G+Y      +G +K+ RG  L EKGV ESLG
Sbjct: 360 LPWN----GKGEAIMVVIDREKKTVEADNGYYYLVV-ADGGMKLDRGLVLKEKGVNESLG 419

Query: 441 TVLLVVRPPKDMEDD-QLSDEDWD 463
            V+LVVRPP+D +D+ Q++DEDWD
Sbjct: 420 MVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of Lag0035551 vs. ExPASy Swiss-Prot
Match: Q9SR19 (Rubisco accumulation factor 1.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RAF1.2 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.1e-118
Identity = 221/388 (56.96%), Postives = 291/388 (75.00%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PP SP+P Q+RSLD+ GK+++L+ R+ LWFEYAPLISSL  +GFTPPTIEE+TGIS ++Q
Sbjct: 70  PPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAPLISSLYTDGFTPPTIEELTGISSIEQ 129

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N LIVGAQVR S++ +I   +P+LI+AFDT GAELLYEIRLLS  +R +AA +I+D  +D
Sbjct: 130 NRLIVGAQVRDSILQSI--HEPELISAFDTGGAELLYEIRLLSTTQRVAAATFIIDRNID 189

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
            KGAQDLARA+KD+P RRGD GW  FDY+ PGDCL+F+YYR SRE KN  + RT+ L  A
Sbjct: 190 SKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCLSFLYYRQSRENKNPSDQRTSMLLQA 249

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAE----EETEAGLKVPVVRLKIGEVAEATTVMVM 320
           L V  +E A++ +  +L G+ E ++  E    EE    +++PVVRLK GEVAEAT+V+V+
Sbjct: 250 LGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEEVKAIRIPVVRLKFGEVAEATSVVVL 309

Query: 321 PVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFND 380
           PVCKAEEGEK +  APME+ + G+F VV AE+GW+RWVVLP+W P+  +G+GGV V+F D
Sbjct: 310 PVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWKRWVVLPSWNPVAAIGKGGVAVSFRD 369

Query: 381 AR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVR 440
            R  LPW      KEE +LVV DR RN V A+DG+YL  A  E  LK+++G+ L  + V+
Sbjct: 370 DRKVLPWD----GKEEPLLVVADRVRNVVEADDGYYLVVA--ENGLKLEKGSDLKAREVK 429

Query: 441 ESLGTVLLVVRPPKDMEDD-QLSDEDWD 463
           ESLG V+LVVRPP++ +DD Q S ++WD
Sbjct: 430 ESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of Lag0035551 vs. ExPASy Swiss-Prot
Match: Q7XDY9 (Rubisco accumulation factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RAF1 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 6.6e-92
Identity = 207/463 (44.71%), Postives = 274/463 (59.18%), Query Frame = 0

Query: 25  PHHALRRRRLSHCHHLPLPFSP--------------PLPPPPSNPHLRHSQPFPFPFPLS 84
           PHH  RRRR +H     +   P              P  PPP+ P  +  QPF       
Sbjct: 32  PHHR-RRRRFAHLTTSAVILGPDGRPIGGGPRDNKLPFTPPPTAPPDQLYQPF------- 91

Query: 85  RKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIE 144
                     PPPSPLP +Y+ LD   +L VL +RLGLW EYAPLIS+L +EGFTP +IE
Sbjct: 92  ---------HPPPSPLPDKYKDLDLGQRLAVLRDRLGLWHEYAPLISALSREGFTPSSIE 151

Query: 145 EITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDT-SGAELLYEIRLLSAEKRAS 204
           E TGISGV+QN ++V  QVR SLV        +L+  FD+  G ELLYE+R L+A +RA 
Sbjct: 152 EATGISGVEQNSVVVATQVRDSLVADEGGFPAELLRYFDSYGGPELLYELRFLNARQRAD 211

Query: 205 AAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQ 264
           AA++ +D RL+ +G ++LAR+MKDFP+RRGD GWE+F  D PGDCLAF  +R SRE    
Sbjct: 212 AARHAIDRRLEPRGVRELARSMKDFPQRRGDDGWEAFTRDNPGDCLAFARFRQSRE-AID 271

Query: 265 PEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGL----------KVPV 324
            E   A LE AL+VV TE AR+ +  +L+       G E + E G            VPV
Sbjct: 272 AEDSVAELERALEVVDTEPARARVEAELDRARRKAAGEEVDDEDGAANAAAAASRPAVPV 331

Query: 325 VRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAW 384
           VRL  GEVAEATTV+++PV +  +G + +  AP   R++ + G+V  ++GW RW V+P W
Sbjct: 332 VRLMYGEVAEATTVLLLPVVREGDGGEALAHAPRRTRTDADLGMVEVDKGWTRWAVVPGW 391

Query: 385 EPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEG 444
            P+  +    VV+   D R LPWR     + E +LVV +R R EVV EDG Y+     EG
Sbjct: 392 GPVAEVAGEAVVIELADGRTLPWRS---AEAERVLVVANRGRREVV-EDGIYVVER--EG 451

Query: 445 ELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
            L V++G  L  +GV E+   VL+VVRPP+D EDD +SD++WD
Sbjct: 452 RLVVEKGRKLAAEGVGEAAAEVLVVVRPPRD-EDDMISDDEWD 469

BLAST of Lag0035551 vs. ExPASy Swiss-Prot
Match: B4FR29 (Rubisco accumulation factor 1, chloroplastic OS=Zea mays OX=4577 GN=RAF1 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.2e-89
Identity = 199/433 (45.96%), Postives = 272/433 (62.82%), Query Frame = 0

Query: 42  LPFSPPLPPPPSNPHLRHSQPFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDV 101
           LPF+PP   PP   +    QPF                 PPPSPLPP  R+LD + +L +
Sbjct: 59  LPFTPPPMAPPGQLY----QPF----------------HPPPSPLPPSLRNLDLSERLQI 118

Query: 102 LSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNS- 161
           L +R+GLW EYAPLISSL ++GF P +I+E TGISGV+QN L+V +QVR SL+     + 
Sbjct: 119 LRDRMGLWHEYAPLISSLSRDGFNPSSIQEATGISGVEQNCLVVASQVRDSLLDDRAAAF 178

Query: 162 DPDLIAAFDT-SGAELLYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRG 221
            PDL+  FD+  G E+LYE+R L+A +RA AA++ +  RL+ KG ++LARAMK FPR RG
Sbjct: 179 PPDLLPYFDSLGGPEVLYELRFLNARQRADAARHAIGYRLEPKGVRELARAMKGFPRWRG 238

Query: 222 DKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLE- 281
           ++GWE+F  D P DCLAF  +R SRE  +  + R A LE AL+VV TE  R+ +  +LE 
Sbjct: 239 EEGWEAFSKDSPADCLAFARFRQSREAIDVQD-RVAELERALQVVETESGRARVELELER 298

Query: 282 ------GEGE-GKEGAEEETEAGLK--VPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVG 341
                 GE E  +EG E++  A L+  V VVRL+ GEVAEATTV+++PV +  +G   + 
Sbjct: 299 ARRKAAGEEEVDEEGEEDDAAASLRPGVTVVRLRYGEVAEATTVILLPVVRETDGVAAME 358

Query: 342 AAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYK 401
           +AP   +++   GVV  +  W RW V+P W P+       VVV   D R LPWR++    
Sbjct: 359 SAPRRAKTDVGLGVVEVDRAWARWAVVPGWGPVAEAADDAVVVELADGRRLPWRMS---D 418

Query: 402 EEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVRESLGTVLLVVRPPK 461
           EE +LV+ +RS+ EVV E+G Y+     EG L V+RG  L E+G+  +   V++VVRPPK
Sbjct: 419 EEPVLVIANRSKKEVV-EEGVYVLER--EGRLVVERGKKLAEQGIAAAAAEVVIVVRPPK 463

Query: 462 DMEDDQLSDEDWD 463
           D EDD +SDE+WD
Sbjct: 479 D-EDDMVSDEEWD 463

BLAST of Lag0035551 vs. ExPASy Swiss-Prot
Match: Q8YLP6 (RuBisCO accumulation factor 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=raf1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.2e-22
Identity = 111/382 (29.06%), Postives = 179/382 (46.86%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PP +P P    +      L  L  + G W E+   I+SL + G+ P  I E TG   VQQ
Sbjct: 5   PPNAPNPENATNELAQELLRKLRQKQGNWVEWGQAIASLQKSGYNPQDIFEATGFEPVQQ 64

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N +IVG+QV  SL  +   +    +A + T G+++LYE+RLL+ E+RA+A      +++D
Sbjct: 65  NQVIVGSQVYNSLEKS--GASAATLAHYATRGSDVLYELRLLTHEERAAAGDLTFTHKVD 124

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
              A+++A+A+KDF R R     E F  + PGD +A+  ++L+R+Y +  E R+  +   
Sbjct: 125 ADEAREIAKAIKDFSRFRILP--EGFS-NHPGDAVAYQAWKLARQYSDLQE-RSRLIARG 184

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLKI-GEVAEATTVMVMPVC 320
           L+   +E AR  I + L       +            P  R     E+     V+     
Sbjct: 185 LRFAHSETARKQIEQLL------VDFTVVSQRPAPIPPFFRFDTEDELPRIVPVVGQLPL 244

Query: 321 KAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARA 380
           KAEE    + A P+    E E   +V   G + WV LP W+ L        ++A +D   
Sbjct: 245 KAEE----LKAVPLV--EEIEPFRLVKFSGEQAWVALPGWQVLLAAEDPVTILATSDR-- 304

Query: 381 LPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVRESLG 440
             +      +   +LVV DRS+ E    + F       +GEL  Q      E  +   LG
Sbjct: 305 --FPKQNQTEPGPVLVVVDRSQRE---WNDFSYFVVDHDGELDFQWFETKPEFPI---LG 357

Query: 441 TVLLVVRPPKDMEDDQLSDEDW 462
            V+++VRP + + D+ ++ + W
Sbjct: 365 KVIILVRPRRIL-DENVTKDSW 357

BLAST of Lag0035551 vs. ExPASy TrEMBL
Match: A0A6J1F451 (rubisco accumulation factor 1.1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439956 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 9.0e-185
Identity = 335/412 (81.31%), Postives = 360/412 (87.38%), Query Frame = 0

Query: 52  PSNPHLRHSQPFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFE 111
           P NP      P P P PL  K        PPPSPLP QYRSLD  GKLDVL+NRLGLWFE
Sbjct: 36  PPNPISASLNPSPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKLDVLANRLGLWFE 95

Query: 112 YAPLISSLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTS 171
           YAPLISSLIQEGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI+++DPDLIAAFDT 
Sbjct: 96  YAPLISSLIQEGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTINDADPDLIAAFDTG 155

Query: 172 GAELLYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFP 231
           GAELLYEIRLL+AEKRA+AA+YIV+NRLDGKGAQDLARAMKDFPRRRGDKGWESF+YD+P
Sbjct: 156 GAELLYEIRLLNAEKRAAAARYIVENRLDGKGAQDLARAMKDFPRRRGDKGWESFNYDYP 215

Query: 232 GDCLAFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEET 291
           GDCLAFM+YRLSREY N  EARTAALEDALKV VTE ARSLIVRDLE EGEGK+  EEE 
Sbjct: 216 GDCLAFMHYRLSREYSNMSEARTAALEDALKVAVTEEARSLIVRDLEREGEGKDEVEEEI 275

Query: 292 EAGLKVPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWR 351
           EAG++VPVVRLKIGEVAEATTV+V+PVC  EEG KGVG APMEVRSEGEFGVVVAEEGWR
Sbjct: 276 EAGVRVPVVRLKIGEVAEATTVVVLPVCNGEEGGKGVGEAPMEVRSEGEFGVVVAEEGWR 335

Query: 352 RWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFY 411
           RWVVLP WEP+ GLG+GGVVVAF DARALPWRVNRWYKEEAILVV DRSR EV A DGFY
Sbjct: 336 RWVVLPGWEPIAGLGKGGVVVAFKDARALPWRVNRWYKEEAILVVADRSRKEVEAGDGFY 395

Query: 412 LT-AAGGEGELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           LT A G +GELKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSDEDWD
Sbjct: 396 LTGATGSDGELKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSDEDWD 447

BLAST of Lag0035551 vs. ExPASy TrEMBL
Match: A0A6J1HLU0 (rubisco accumulation factor 1.1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465376 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 6.5e-183
Identity = 335/424 (79.01%), Postives = 363/424 (85.61%), Query Frame = 0

Query: 48  LPPPPSNPHLRHS--------QPFPFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKL 107
           L  PP    LRH          PFP P PL  K        PPPSPLP QYRSLD  GKL
Sbjct: 57  LHTPPLRLFLRHPPNLISASLNPFPSPSPLVAKPQVYQPFRPPPSPLPSQYRSLDAAGKL 116

Query: 108 DVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDN 167
           DVL+NRLGLWFEYAPLISSLIQEGFTPPTIEEITGI GVQQN L+VGAQVR+SLV TI++
Sbjct: 117 DVLANRLGLWFEYAPLISSLIQEGFTPPTIEEITGIGGVQQNTLVVGAQVRESLVQTIND 176

Query: 168 SDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRG 227
           +DPDLIAAFDT GAELLYEIRLL+AEKRA+AA+YI++NRLDGKGAQDLARAMKDFPRRRG
Sbjct: 177 TDPDLIAAFDTGGAELLYEIRLLNAEKRAAAARYIMENRLDGKGAQDLARAMKDFPRRRG 236

Query: 228 DKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDALKVVVTEGARSLIVRDLEG 287
           DKGWESF+YD+PGDCLAFM+YRLSREY N  EARTAAL DALKV VTE ARSLIVRDLE 
Sbjct: 237 DKGWESFNYDYPGDCLAFMHYRLSREYSNMSEARTAALADALKVAVTEEARSLIVRDLER 296

Query: 288 EGEGKEGAEEETEAGLKVPVVRLKIGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEG 347
           EGEGK+GAEEE EAG++VPVVRLKIGEVAEATTV+V+PVC  EEG KGV  APMEVRSEG
Sbjct: 297 EGEGKDGAEEEIEAGVRVPVVRLKIGEVAEATTVVVLPVCNGEEGGKGVREAPMEVRSEG 356

Query: 348 EFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDARALPWRVNRWYKEEAILVVGDR 407
           EFGVVVAEEGWRRWVVLP WEP+ GLG+GGVVV F DAR+LPWRVNRWYKEEAILVV DR
Sbjct: 357 EFGVVVAEEGWRRWVVLPGWEPIAGLGKGGVVVVFKDARSLPWRVNRWYKEEAILVVADR 416

Query: 408 SRNEVVAEDGFYLT-AAGGEGELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSD 463
           SR EV A DGFY+T A G +GELKVQRG+ LME G++ESLGTVLLVVRPPKDM+DDQLSD
Sbjct: 417 SRKEVEAGDGFYMTGATGNDGELKVQRGSELMEMGLKESLGTVLLVVRPPKDMDDDQLSD 476

BLAST of Lag0035551 vs. ExPASy TrEMBL
Match: A0A5D3DZE4 (Rubisco accumulation factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001420 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.8e-172
Identity = 318/405 (78.52%), Postives = 350/405 (86.42%), Query Frame = 0

Query: 64  PFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEG 123
           P P PL+ K        PPPSPLPPQYRSLDT GKL+VLSNRLGLWFEYAPLISSL+QEG
Sbjct: 51  PSPSPLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFEYAPLISSLLQEG 110

Query: 124 FTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLS 183
           FTPPT+EEITGISGVQQN  +VGAQVR+SLV + D SDPD+IA+FDTSGAELLYEIRLLS
Sbjct: 111 FTPPTLEEITGISGVQQNTFVVGAQVRESLVQSND-SDPDVIASFDTSGAELLYEIRLLS 170

Query: 184 AEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLS 243
            EKR +AAKYIV+NRLD KGAQDLARA+KDFPRR+GD+GWE FDYD  GDCLA+MYYRLS
Sbjct: 171 TEKRVAAAKYIVENRLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLS 230

Query: 244 REYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLK 303
           REY N  E RTAALE+ALKVVVTE AR+LIV DLE +G+GK+G EEET AG++VPVVRLK
Sbjct: 231 REYNNSSERRTAALEEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLK 290

Query: 304 IGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLR 363
           IGEVAEAT V+VMPVCKA EGEKGVG APMEVRSEG+FGVVVAE+GW RWVVLP WEP+ 
Sbjct: 291 IGEVAEATKVVVMPVCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVA 350

Query: 364 GLGR-GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYL---TAAGGE- 423
           GL + GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   T  GG+ 
Sbjct: 351 GLVKGGGVVVAFKDARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDG 410

Query: 424 -GELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
            GELKV+RGNAL E GV+ESLGTV+LVVRPPK+MEDDQLSDEDWD
Sbjct: 411 GGELKVERGNALTEMGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of Lag0035551 vs. ExPASy TrEMBL
Match: A0A1S3CHL8 (rubisco accumulation factor 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501013 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.9e-172
Identity = 317/405 (78.27%), Postives = 350/405 (86.42%), Query Frame = 0

Query: 64  PFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEG 123
           P P PL+ K        PPPSPLPPQYRSLDT GKL+VLSNRLGLWF+YAPLISSL+QEG
Sbjct: 51  PSPSPLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNVLSNRLGLWFQYAPLISSLLQEG 110

Query: 124 FTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLS 183
           FTPPT+EEITGISGVQQN  +VGAQVR+SLV + D SDPD+IA+FDTSGAELLYEIRLLS
Sbjct: 111 FTPPTLEEITGISGVQQNTFVVGAQVRESLVQSND-SDPDVIASFDTSGAELLYEIRLLS 170

Query: 184 AEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLS 243
            EKR +AAKYIV+NRLD KGAQDLARA+KDFPRR+GD+GWE FDYD  GDCLA+MYYRLS
Sbjct: 171 TEKRVAAAKYIVENRLDSKGAQDLARAIKDFPRRKGDRGWECFDYDSAGDCLAYMYYRLS 230

Query: 244 REYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLK 303
           REY N  E RTAALE+ALKVVVTE AR+LIV DLE +G+GK+G EEET AG++VPVVRLK
Sbjct: 231 REYNNSSERRTAALEEALKVVVTEKARNLIVGDLERKGDGKDGVEEETGAGVRVPVVRLK 290

Query: 304 IGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLR 363
           IGEVAEAT V+VMPVCKA EGEKGVG APMEVRSEG+FGVVVAE+GW RWVVLP WEP+ 
Sbjct: 291 IGEVAEATKVVVMPVCKAGEGEKGVGEAPMEVRSEGDFGVVVAEKGWSRWVVLPGWEPVA 350

Query: 364 GLGR-GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYL---TAAGGE- 423
           GL + GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   T  GG+ 
Sbjct: 351 GLVKGGGVVVAFKDARVLPWRVNRWYKEEPILVVADRSRKEVVAGDGFYLMGGTVGGGDG 410

Query: 424 -GELKVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
            GELKV+RGNAL E GV+ESLGTV+LVVRPPK+MEDDQLSDEDWD
Sbjct: 411 GGELKVERGNALTEMGVKESLGTVVLVVRPPKEMEDDQLSDEDWD 454

BLAST of Lag0035551 vs. ExPASy TrEMBL
Match: A0A0A0LY43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690280 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 8.8e-172
Identity = 316/401 (78.80%), Postives = 345/401 (86.03%), Query Frame = 0

Query: 64  PFPFPLSRKATSLPALPPPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEG 123
           P    L+ K        PPPSPLPPQYRSLDT GKL++LSNRLGLWFEYAPLISSL+QEG
Sbjct: 51  PSSSSLAAKQQVYQPFRPPPSPLPPQYRSLDTEGKLNILSNRLGLWFEYAPLISSLLQEG 110

Query: 124 FTPPTIEEITGISGVQQNILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLS 183
           FTPP +EEITGISGVQQN  IVGAQVR+SL+ + D SDPD+IA+FDT GAELLYEIRLLS
Sbjct: 111 FTPPVLEEITGISGVQQNSFIVGAQVRESLLQSND-SDPDVIASFDTGGAELLYEIRLLS 170

Query: 184 AEKRASAAKYIVDNRLDGKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLS 243
            EKRA+AAKYIV+NRLD KGAQDLARAMKDFPRRRGDKGWE FDYDF GDCLA+MYYRLS
Sbjct: 171 TEKRAAAAKYIVENRLDSKGAQDLARAMKDFPRRRGDKGWEYFDYDFAGDCLAYMYYRLS 230

Query: 244 REYKNQPEARTAALEDALKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLK 303
           REY +  E RTAALE+ALKVVVTE AR LIV DLEG+G+GK+G EEE  A +KVPVVR+K
Sbjct: 231 REYNSSTERRTAALEEALKVVVTEKARDLIVGDLEGKGDGKDGVEEEIGAAVKVPVVRMK 290

Query: 304 IGEVAEATTVMVMPVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLR 363
           IGEVAEATTV+VMPVCKA EGEKGVG APMEVRSEGEFGVVVAE+GW RWVVLP WEP+ 
Sbjct: 291 IGEVAEATTVVVMPVCKAGEGEKGVGEAPMEVRSEGEFGVVVAEKGWSRWVVLPGWEPVA 350

Query: 364 GLGR-GGVVVAFNDARALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAG-GEGEL 423
           GL + GGVVVAF DAR LPWRVNRWYKEE ILVV DRSR EVVA DGFYL   G G G+L
Sbjct: 351 GLVKGGGVVVAFEDARVLPWRVNRWYKEEPILVVADRSRREVVAGDGFYLMGGGDGGGDL 410

Query: 424 KVQRGNALMEKGVRESLGTVLLVVRPPKDMEDDQLSDEDWD 463
           KV+RGNALME GV+ESLGTVLLVVRPP++ EDDQLSDEDWD
Sbjct: 411 KVERGNALMEMGVKESLGTVLLVVRPPRETEDDQLSDEDWD 450

BLAST of Lag0035551 vs. TAIR 10
Match: AT5G28500.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1); Has 109 Blast hits to 109 proteins in 49 species: Archae - 0; Bacteria - 67; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-121
Identity = 231/384 (60.16%), Postives = 292/384 (76.04%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+TGISGV+Q
Sbjct: 60  PPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISGVEQ 119

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N LIVGAQVR SLV +   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+YIVD+  D
Sbjct: 120 NSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHGFD 179

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
            KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E RT  LE A
Sbjct: 180 TKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLEQA 239

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAEEETEAGLKVPVVRLKIGEVAEATTVMVMPVCK 320
           L+  VTE A+  ++R+L GE E +   EEE +  ++VPVVRL+ GEVA A++V+V+PVCK
Sbjct: 240 LETAVTEKAKKAVLRELHGESEEERVKEEEIKI-IRVPVVRLRFGEVAGASSVVVLPVCK 299

Query: 321 AEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFNDAR-A 380
           AEEGE+ +  APME  S GEFGVV AE+ W RWVVLP W+P+  + R GV V+F+D R  
Sbjct: 300 AEEGEEKLLEAPMEFESGGEFGVVEAEKDWSRWVVLPGWDPVVAV-RKGVAVSFSDDREV 359

Query: 381 LPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVRESLG 440
           LPW      K EAI+VV DR +  V A++G+Y      +G +K+ RG  L EKGV ESLG
Sbjct: 360 LPWN----GKGEAIMVVIDREKKTVEADNGYYYLVV-ADGGMKLDRGLVLKEKGVNESLG 419

Query: 441 TVLLVVRPPKDMEDD-QLSDEDWD 463
            V+LVVRPP+D +D+ Q++DEDWD
Sbjct: 420 MVVLVVRPPRDDDDEWQINDEDWD 434

BLAST of Lag0035551 vs. TAIR 10
Match: AT3G04550.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28500.1); Has 110 Blast hits to 110 proteins in 51 species: Archae - 0; Bacteria - 67; Metazoa - 1; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 426.4 bits (1095), Expect = 2.9e-119
Identity = 221/388 (56.96%), Postives = 291/388 (75.00%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PP SP+P Q+RSLD+ GK+++L+ R+ LWFEYAPLISSL  +GFTPPTIEE+TGIS ++Q
Sbjct: 70  PPSSPIPTQFRSLDSAGKIEILAGRMALWFEYAPLISSLYTDGFTPPTIEELTGISSIEQ 129

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N LIVGAQVR S++ +I   +P+LI+AFDT GAELLYEIRLLS  +R +AA +I+D  +D
Sbjct: 130 NRLIVGAQVRDSILQSI--HEPELISAFDTGGAELLYEIRLLSTTQRVAAATFIIDRNID 189

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
            KGAQDLARA+KD+P RRGD GW  FDY+ PGDCL+F+YYR SRE KN  + RT+ L  A
Sbjct: 190 SKGAQDLARAIKDYPNRRGDVGWLDFDYNLPGDCLSFLYYRQSRENKNPSDQRTSMLLQA 249

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAE----EETEAGLKVPVVRLKIGEVAEATTVMVM 320
           L V  +E A++ +  +L G+ E ++  E    EE    +++PVVRLK GEVAEAT+V+V+
Sbjct: 250 LGVAESEKAKNRLNTELYGDKEAEKEKEKKKKEEEVKAIRIPVVRLKFGEVAEATSVVVL 309

Query: 321 PVCKAEEGEKGVGAAPMEVRSEGEFGVVVAEEGWRRWVVLPAWEPLRGLGRGGVVVAFND 380
           PVCKAEEGEK +  APME+ + G+F VV AE+GW+RWVVLP+W P+  +G+GGV V+F D
Sbjct: 310 PVCKAEEGEKKILEAPMEIIAGGDFKVVEAEKGWKRWVVLPSWNPVAAIGKGGVAVSFRD 369

Query: 381 AR-ALPWRVNRWYKEEAILVVGDRSRNEVVAEDGFYLTAAGGEGELKVQRGNALMEKGVR 440
            R  LPW      KEE +LVV DR RN V A+DG+YL  A  E  LK+++G+ L  + V+
Sbjct: 370 DRKVLPWD----GKEEPLLVVADRVRNVVEADDGYYLVVA--ENGLKLEKGSDLKAREVK 429

Query: 441 ESLGTVLLVVRPPKDMEDD-QLSDEDWD 463
           ESLG V+LVVRPP++ +DD Q S ++WD
Sbjct: 430 ESLGMVVLVVRPPREDDDDWQTSHQNWD 449

BLAST of Lag0035551 vs. TAIR 10
Match: AT5G28500.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G04550.1). )

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-72
Identity = 136/209 (65.07%), Postives = 168/209 (80.38%), Query Frame = 0

Query: 81  PPPSPLPPQYRSLDTNGKLDVLSNRLGLWFEYAPLISSLIQEGFTPPTIEEITGISGVQQ 140
           PPPSP+PP++RSLDT GK++VL++RLGLWFEYAPLISSL  EGFTPP+IEE+TGISGV+Q
Sbjct: 60  PPPSPIPPKFRSLDTAGKIEVLADRLGLWFEYAPLISSLYTEGFTPPSIEELTGISGVEQ 119

Query: 141 NILIVGAQVRQSLVHTIDNSDPDLIAAFDTSGAELLYEIRLLSAEKRASAAKYIVDNRLD 200
           N LIVGAQVR SLV +   + P+LIAAFDT+GAELLYEIRLL+  +R +AA+YIVD+  D
Sbjct: 120 NSLIVGAQVRDSLVQS--GAKPELIAAFDTNGAELLYEIRLLNTTQRVAAAEYIVDHGFD 179

Query: 201 GKGAQDLARAMKDFPRRRGDKGWESFDYDFPGDCLAFMYYRLSREYKNQPEARTAALEDA 260
            KGA DLARA+KDFP RRGD G   FDY+ PGDCL+FM YR SRE+++  E RT  LE A
Sbjct: 180 TKGAGDLARAIKDFPHRRGDVGLGDFDYNLPGDCLSFMLYRKSREHRSPSEIRTTLLEQA 239

Query: 261 LKVVVTEGARSLIVRDLEGEGEGKEGAEE 290
           L+  VTE A+  ++R+L GE E + G E+
Sbjct: 240 LETAVTEKAKKAVLRELHGESEEERGKED 266