Lag0035521 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035521
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H
Locationchr3: 23287939 .. 23289729 (+)
RNA-Seq ExpressionLag0035521
SyntenyLag0035521
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCCTCCCGGGGACGACCAGACCCAGAAGGAAGCTGGACCAAGCCGCAAAAAGACCCGCATGAATTCTCCGCTAAAGACAGCGCCATGTACGTACATAGGGAATAATGACAGGAAAAAGTTGGAGGCTCGGGCAAAGTTCGAGGTCGAGCAGGGCCAAAAGAGACGAGAGCCATCCAAGTGGCTAAAAGAGGAAGATAGTTACCAAACCTCCCAAAAGAGGACAGAGAACGAGGACATAGAAGGACTGATTGGGCAGGTGGGGCCAGTCTTCACCGACGAAATAATGGAGGAGAGGTGCCGCACAAATTCAAAATACCAAACTTCCCGCAGTATGATGGGAAGAAGGACCCGAAGCAGCACCTAGATGCATACCAAACCTGGATGGACTTTCACGGGGCGAACGAAGCAACAAGGTGCCGAGCCTTTGCACTAACACTCATAGATTTGGCAAGGCAATGGTTTGGCAAGATCTCGCGAAAGTCAATTGGTTCGTTCAGAGAGCTGACCGAGAGTTTGTTACGCAATTTCTAGGAGCCCGTAGCCAACAAAAGCCTCAGATCAACTTGCTGACAGTAAACAGGGGCCCCAGAAAGCCTGAAGGATTATATTAACAGATTTAGTAACGAAGCTTTGCAGGTGGAGGGTTACGACGACGGAGTTGCCTTGGCCGCTGTGATTTCAGGTTTGCAAGATGAGAGACTGCTCAACTCAATCGGAGAGAGCCAACCACGAACGTATGTGGAGTTTATGACCCGAGCACAAAGATACATAAGCGCCGAGGAACTATTGAGGTCCAAACAGGAAGAAAGAGAGAGTCGGAGGGTGGCAATATCAACCGGCATCGAGAAGACAGAGGAAAGAGGCACCAGGTCGAGGGAAGAGGCCAAAGCCGACTTGAGCACTCTTCGGCCAATGGCCGAGGCCGACCAGAGGCCAAGGAGCTGCAGGGCCGTGCGGAACCAAGAGCCAGATTCGACATGTATACACCACTAACAGCCTCGCTTGAACAAGTCTTGGTCGCAATACAAGACACGAATCTGTTGAAACGCCCAGAAAAGCTGAGATCAGACCCCGACAGGAGGAATAGAAACAAATACTGTATGTTCCACGGAGACCACGGTCACACAACCCGGGAATGCATACAATTGAAGGACGAGATAGAAGCCCTAATTCGAGAAGGTTACCTCAAGGAGTTCGTGGGGAATGACAGAAGCAAGAGGCCACTGCCAGCAGATCAAGGTAAGGGCGGTGCCAACCCACCGCTCGAGATACGAACAATTTTAGGAGGACCATCCGGAGGAGAGTCAGGTAGAAAGCGAAAGGCTGCAATTCGAGAGGCACAACAAGGGCCTAGAGAGCAAGGTATGTACTGACTCCTACTCGATGAAAACTCACAAAGTTAGAGTTTACAAAGAAAGAGGCCGTAGGGATACGTCATCCGCACAACGATGCGCTGGTAGTCACTCTAACGATTGCCAATGCAAAAGTTCACCGGATCCTCGTTGATGGGGGGAGTTCTGCTGATGTGCTCTCAACCACTGCGTTCGACGCCATAAAGCTGGGAAGCGATCGCCCGAGGTCGAGCCTCACGTCGCTGGTGGGGTTTGACAGAGAAAAAGTAAGCCCAAGAGGAAACGTCGAACTGTCGGTGACGTTTGGGGAAGGACCGCAGACAGTAACAAGGATGATCAACTTTCTAGTGGTAGACTACGTCCCAACATATAATGCCATCTTGGGACGACCCACCCTACATGGACTTAAAGCTGTAGCCACAACCTACCACTAA

mRNA sequence

ATGCCTCCTCCCGGGGACGACCAGACCCAGAAGGAAGCTGGACCAAGCCGCAAAAAGACCCGCATGAATTCTCCGCTAAAGACAGCGCCATGTACGTACATAGGGAATAATGACAGGAAAAAGTTGGAGGCTCGGGCAAAGTTCGAGGTCGAGCAGGGCCAAAAGAGACGAGAGCCATCCAAGTGGCTAAAAGAGGAAGATAGTTACCAAACCTCCCAAAAGAGGACAGAGAACGAGGACATAGAAGGACTGATTGGGCAGTATGATGGGAAGAAGGACCCGAAGCAGCACCTAGATGCATACCAAACCTGGATGGACTTTCACGGGGCGAACGAAGCAACAAGGTGCCGAGCCTTTGCACTAACACTCATAGATTTGGCAAGGCAATGGTTTGGCAAGATCTCGCGAAAGTCAATTGGGGCCCCAGAAAGCCTGAAGGATTATATTAACAGATTTAGTAACGAAGCTTTGCAGGTGGAGGGTTACGACGACGGAGTTGCCTTGGCCGCTGTGATTTCAGGAAGAAAGAGAGAGTCGGAGGGTGGCAATATCAACCGGCATCGAGAAGACAGAGGAAAGAGGCACCAGGTCGAGGGAAGAGGCCAAAGCCGACTTGAGCACTCTTCGGCCAATGGCCGAGGCCGACCAGAGGCCAAGGAGCTGCAGGGCCGTGCGGAACCAAGAGCCAGATTCGACATGTATACACCACTAACAGCCTCGCTTGAACAAGTCTTGGTCGCAATACAAGACACGAATCTGTTGAAACGCCCAGAAAAGCTGAGATCAGACCCCGACAGGAGGAATAGAAACAAATACTGTATGTTCCACGGAGACCACGGTCACACAACCCGGGAATGCATACAATTGAAGGACGAGATAGAAGCCCTAATTCGAGAAGGTTACCTCAAGGAGTTCGTGGGGAATGACAGAAGCAAGAGGCCACTGCCAGCAGATCAAGGTAAGGGCGGTGCCAACCCACCGCTCGAGATACGAACAATTTTAGGAGGACCATCCGGAGGAGAGTCAGGTAGAAAGCGAAAGGCTGCAATTCGAGAGGCACAACAAGGGCCTAGAGAGCAAGAGGCCGTAGGGATACGTCATCCGCACAACGATGCGCTGGTAGTCACTCTAACGATTGCCAATGCAAAAGTTCACCGGATCCTCGTTGATGGGGGGAGTTCTGCTGATGTGCTCTCAACCACTGCGTTCGACGCCATAAAGCTGGGAAGCGATCGCCCGAGGTCGAGCCTCACGTCGCTGGTGGGGTTTGACAGAGAAAAAGTAAGCCCAAGAGGAAACGTCGAACTGTCGGTGACGTTTGGGGAAGGACCGCAGACAGTAACAAGGATGATCAACTTTCTAGTGGTAGACTACGTCCCAACATATAATGCCATCTTGGGACGACCCACCCTACATGGACTTAAAGCTGTAGCCACAACCTACCACTAA

Coding sequence (CDS)

ATGCCTCCTCCCGGGGACGACCAGACCCAGAAGGAAGCTGGACCAAGCCGCAAAAAGACCCGCATGAATTCTCCGCTAAAGACAGCGCCATGTACGTACATAGGGAATAATGACAGGAAAAAGTTGGAGGCTCGGGCAAAGTTCGAGGTCGAGCAGGGCCAAAAGAGACGAGAGCCATCCAAGTGGCTAAAAGAGGAAGATAGTTACCAAACCTCCCAAAAGAGGACAGAGAACGAGGACATAGAAGGACTGATTGGGCAGTATGATGGGAAGAAGGACCCGAAGCAGCACCTAGATGCATACCAAACCTGGATGGACTTTCACGGGGCGAACGAAGCAACAAGGTGCCGAGCCTTTGCACTAACACTCATAGATTTGGCAAGGCAATGGTTTGGCAAGATCTCGCGAAAGTCAATTGGGGCCCCAGAAAGCCTGAAGGATTATATTAACAGATTTAGTAACGAAGCTTTGCAGGTGGAGGGTTACGACGACGGAGTTGCCTTGGCCGCTGTGATTTCAGGAAGAAAGAGAGAGTCGGAGGGTGGCAATATCAACCGGCATCGAGAAGACAGAGGAAAGAGGCACCAGGTCGAGGGAAGAGGCCAAAGCCGACTTGAGCACTCTTCGGCCAATGGCCGAGGCCGACCAGAGGCCAAGGAGCTGCAGGGCCGTGCGGAACCAAGAGCCAGATTCGACATGTATACACCACTAACAGCCTCGCTTGAACAAGTCTTGGTCGCAATACAAGACACGAATCTGTTGAAACGCCCAGAAAAGCTGAGATCAGACCCCGACAGGAGGAATAGAAACAAATACTGTATGTTCCACGGAGACCACGGTCACACAACCCGGGAATGCATACAATTGAAGGACGAGATAGAAGCCCTAATTCGAGAAGGTTACCTCAAGGAGTTCGTGGGGAATGACAGAAGCAAGAGGCCACTGCCAGCAGATCAAGGTAAGGGCGGTGCCAACCCACCGCTCGAGATACGAACAATTTTAGGAGGACCATCCGGAGGAGAGTCAGGTAGAAAGCGAAAGGCTGCAATTCGAGAGGCACAACAAGGGCCTAGAGAGCAAGAGGCCGTAGGGATACGTCATCCGCACAACGATGCGCTGGTAGTCACTCTAACGATTGCCAATGCAAAAGTTCACCGGATCCTCGTTGATGGGGGGAGTTCTGCTGATGTGCTCTCAACCACTGCGTTCGACGCCATAAAGCTGGGAAGCGATCGCCCGAGGTCGAGCCTCACGTCGCTGGTGGGGTTTGACAGAGAAAAAGTAAGCCCAAGAGGAAACGTCGAACTGTCGGTGACGTTTGGGGAAGGACCGCAGACAGTAACAAGGATGATCAACTTTCTAGTGGTAGACTACGTCCCAACATATAATGCCATCTTGGGACGACCCACCCTACATGGACTTAAAGCTGTAGCCACAACCTACCACTAA

Protein sequence

MPPPGDDQTQKEAGPSRKKTRMNSPLKTAPCTYIGNNDRKKLEARAKFEVEQGQKRREPSKWLKEEDSYQTSQKRTENEDIEGLIGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSIGAPESLKDYINRFSNEALQVEGYDDGVALAAVISGRKRESEGGNINRHREDRGKRHQVEGRGQSRLEHSSANGRGRPEAKELQGRAEPRARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPPLEIRTILGGPSGGESGRKRKAAIREAQQGPREQEAVGIRHPHNDALVVTLTIANAKVHRILVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVSPRGNVELSVTFGEGPQTVTRMINFLVVDYVPTYNAILGRPTLHGLKAVATTYH
Homology
BLAST of Lag0035521 vs. NCBI nr
Match: XP_030955724.1 (uncharacterized protein LOC115977839 [Quercus lobata])

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-70
Identity = 177/461 (38.39%), Postives = 236/461 (51.19%), Query Frame = 0

Query: 85  IGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSI----- 144
           +  YDG +DP  H+  ++T M   G  +   CRAF  TL   AR WF KI   S+     
Sbjct: 50  LDSYDGTRDPFDHIATFKTTMHLQGVPDEIMCRAFPTTLKGPARVWFSKIPPSSVSSFEE 109

Query: 145 ---------------------------GAPESLKDYINRFSNEALQVEGYDDGVALAAVI 204
                                      G  ESL+ +I RF+ EAL V+  DD + LAA  
Sbjct: 110 LSKLFVNNFIGGQRHKRSSSSLLTIEQGENESLRSFITRFNREALSVDEVDDKLLLAAFH 169

Query: 205 SG-----------RKRESEGGNINRHREDRGKRHQVEGRGQSRLEHSSANGRGRPE-AKE 264
           +G            K       + R R +R + H V        E +    +GR E  KE
Sbjct: 170 NGINSDLFIHKLYEKEPQTMAELKRKRAERMEAHPVR-----HSEQAPRPKKGRTEDRKE 229

Query: 265 LQGR-AEPRARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDH 324
             GR   P  R   YTPL A L QVL+ I+D   LK PEK++ DP++RN+NKYC FH DH
Sbjct: 230 RDGRKTGPLGRSQNYTPLNAPLNQVLMQIKDDPSLKWPEKMKGDPNKRNKNKYCRFHRDH 289

Query: 325 GHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPPLEIRTILGGPSG 384
           GH T EC  LK +IE LIR+G LK FVG DR+   L     +    P  EIR I+GG   
Sbjct: 290 GHDTDECYDLKQQIENLIRQGKLKHFVGRDRTDEKLKGKMEESSRPPLGEIRIIVGGNPM 349

Query: 385 GESGRKRKAAIREAQ------QGPR------------EQEAVGIRHPHNDALVVTLTIAN 444
           G+S + +K  ++  Q      + PR            +++A  I HPH+DA+V+TL IA+
Sbjct: 350 GQSSKSKKTYLKAVQNVQLSGRPPRTRFMDEPTISFTDEDAERIHHPHDDAIVITLLIAD 409

Query: 445 AKVHRILVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVSPRGNVELSVTFG 483
               R+LVD GSSAD+L    F  ++LG D+ R   + L+GF   KV P G + L V  G
Sbjct: 410 YTTRRVLVDNGSSADILYYPTFQQMRLGRDQLRPVCSPLIGFKGMKVQPVGTITLPVVVG 469

BLAST of Lag0035521 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 275.4 bits (703), Expect = 9.3e-70
Identity = 168/428 (39.25%), Postives = 229/428 (53.50%), Query Frame = 0

Query: 85  IGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSIGA--- 144
           + Q+D   DP  HLDAY+ WMD +G +EA RCR F+ TL   AR WF ++ R SI +   
Sbjct: 198 VKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFSTTLNGSARIWFRQLKRGSISSFKS 257

Query: 145 -----------------------------PESLKDYINRFSNEALQVEGYDDGVALAAVI 204
                                         ESL+DY+ RF+ E LQVEG  D V+L A +
Sbjct: 258 LARAFVTQFVGGRCRSRPVAYLLTIKQRTTESLRDYVARFNEEKLQVEGLTDAVSLLAFM 317

Query: 205 SGRKRESEGGNINRHRED-------RGKRHQVEG------------RGQSRLEHSSANGR 264
           SG + E    +  +   +       R +R+   G            R   + E S    +
Sbjct: 318 SGVRDEHLSFSFGKRTPNTFSEALSRAQRYMSAGEFFYSKREPDGKRTDPKRERSGDKPQ 377

Query: 265 G-RPEAKELQGRAEPRARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKY 324
           G R E ++   + +P  +F+ YTP T  +EQVL+ I+D  LLK PE++++   +R++ +Y
Sbjct: 378 GSRWEKRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRLLKWPERMKASSAKRSKGRY 437

Query: 325 CMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPPLEIRT 384
           C+FH DHGH T++C  LK+E+E LIR GYLKE+V     + P     G+   +P  EIRT
Sbjct: 438 CLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYV-----EEPKATQNGESDKSPAREIRT 497

Query: 385 ILGGPSGGESGRKRKAAIREAQQGPR-------------------EQEAVGIRHPHNDAL 442
           I+GGP   ESGRKRKA +REA+                       E EA  + HPHNDAL
Sbjct: 498 IMGGPIERESGRKRKADVREARTSREQNEVYHAYTTNRPVTIEFSEDEATHLLHPHNDAL 557

BLAST of Lag0035521 vs. NCBI nr
Match: XP_030930616.1 (uncharacterized protein LOC115956348 [Quercus lobata])

HSP 1 Score: 274.2 bits (700), Expect = 2.1e-69
Identity = 181/473 (38.27%), Postives = 243/473 (51.37%), Query Frame = 0

Query: 85  IGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSI----- 144
           +  YDG +DP  H+  ++T M   G  +   CRAF  TL  LAR WF KI   S+     
Sbjct: 157 LDSYDGTRDPFDHIATFKTTMHLQGVPDEIMCRAFPTTLKGLARVWFSKIPPNSMSSFEE 216

Query: 145 ---------------------------GAPESLKDYINRFSNEALQVEGYDDGVALAAVI 204
                                      G  ESL+ +I RF+ EAL V+  DD + LAA  
Sbjct: 217 LSKLFVNNFIGGQRHKCSSSSLLTIEQGENESLRSFITRFNREALSVDEADDKLLLAAFH 276

Query: 205 SG--------RKRESEGGNINR---------HRED------RGKRHQVEGRGQSRLEHSS 264
           +G        +  E E  ++           + ED      R +  +V+      LE   
Sbjct: 277 NGVSSDLFIHKLYEKEPQSMAELVHSAQNFMNAEDAIIVKKRKRYERVDTNPSRHLEQGP 336

Query: 265 ANGRGRPEAKELQGRAEP--RARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRR 324
              +GR E ++ +   +P   AR   YT L   LEQVL+ I+D   LK PEK+R DP++R
Sbjct: 337 RPKKGRMEDRKDRDIKKPGSSARNQQYTSLNVPLEQVLMQIKDDPSLKWPEKMRGDPNKR 396

Query: 325 NRNKYCMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPP 384
           NRNKYC FH DHGH T EC  LK +IE LIR+G LK F+G D     +     +    P 
Sbjct: 397 NRNKYCRFHRDHGHDTDECFDLKQQIENLIRQGKLKNFLGRDNRDEKMKGKMEESSLPPL 456

Query: 385 LEIRTILGGPSGGESGRKRKAAIREAQ------QGPR------------EQEAVGIRHPH 444
            EIR I+GG S G+S + +KA ++  Q      + PR            +++A  I HPH
Sbjct: 457 EEIRVIVGGSSTGQSSKSKKAYLKVVQSVQLSGRSPRARTTDEAAITFTDEDAERIHHPH 516

Query: 445 NDALVVTLTIANAKVHRILVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVS 483
           +DALV+TL IA+    R+LVD GSS D+L   AF  ++LG DR R   + LVGF R KV 
Sbjct: 517 DDALVITLMIADYTTRRVLVDNGSSTDILYYPAFQQVRLGRDRLRPVNSPLVGFGRMKVQ 576

BLAST of Lag0035521 vs. NCBI nr
Match: XP_024041095.1 (uncharacterized protein LOC112098853 [Citrus clementina])

HSP 1 Score: 273.1 bits (697), Expect = 4.6e-69
Identity = 174/493 (35.29%), Postives = 260/493 (52.74%), Query Frame = 0

Query: 88  YDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSI-------- 147
           YDG++DP +HL+ Y+T M+  GA++A  CRAF LTL+  AR+WF ++   SI        
Sbjct: 198 YDGQRDPTEHLELYRTLMEVQGASQAILCRAFPLTLVGAARRWFRRLQPHSISSFGDLSR 257

Query: 148 ------------GAP------------ESLKDYINRFSNEALQVEGYDDGVALAAVISGR 207
                       G P            E+L+DYI R++NE  QV+GYDDG+AL+ ++ G 
Sbjct: 258 EFTSHFRSARQRGKPVTYLLTVKQQEGETLRDYIRRYNNEITQVDGYDDGIALSGIMEGL 317

Query: 208 K---------------------RESEGGNI-----NRHRE----DRGKRHQVEGRGQS-- 267
           +                     R  +  N      NR++E     +GK+ + + R +   
Sbjct: 318 RMGKLWWSVSKRPPTSYSEMLARAEKYANAEERFKNRNQEKGESTKGKKKKDDQRERRVV 377

Query: 268 RLEHSSANGRGRPEAKELQGRAEPR--ARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLR 327
           R + S    + RP  +       PR  ++F  +T L    EQ+L+ +++  L + P  ++
Sbjct: 378 RPDQSLRRNQFRPGERSEVRDTRPRLASQFTNFTELNTPREQILMQVRNPTLFRTPPPMK 437

Query: 328 SDPDRRNRNKYCMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGND----RSKRPLPA 387
           ++P RRN NKYC FH DHGH T EC +LK++IE+L+R+G L+E+V N     +S++P  +
Sbjct: 438 TNPSRRNPNKYCHFHKDHGHDTSECFELKEQIESLVRQGQLQEYVRNPEDRVKSEQPESS 497

Query: 388 DQGKG-----GANPPLEIRTILGGPSGGESGRKRKAAIREAQQGPR-------------- 447
            + KG           ++  I GGP+ G+S + RK   R+A+  P               
Sbjct: 498 KKRKGKKKEDSDESMSDVNHIWGGPAAGDSRKSRKNLARQARHEPNGPEVNLTNQSQGSM 557

Query: 448 ---------EQEAVGIRHPHNDALVVTLTIANAKVHRILVDGGSSADVLSTTAFDAIKLG 483
                    E +A G+ HPH DALVVTL +AN ++HRIL+D GSSAD+L  + F  + L 
Sbjct: 558 VRGIPIIFSEDDARGVHHPHCDALVVTLPVANKRLHRILIDNGSSADILFLSTFARMGLE 617

BLAST of Lag0035521 vs. NCBI nr
Match: XP_023916366.1 (uncharacterized protein LOC112027956 [Quercus suber])

HSP 1 Score: 265.0 bits (676), Expect = 1.3e-66
Identity = 175/472 (37.08%), Postives = 239/472 (50.64%), Query Frame = 0

Query: 85  IGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSI----- 144
           +  YDG +DP  H+  ++T M   G  +   CRAF  TL   AR WF KI   S+     
Sbjct: 156 LDSYDGTRDPFDHIATFKTTMHLQGVPDEIMCRAFPTTLKGPARVWFSKIPPNSVTSFEE 215

Query: 145 ---------------------------GAPESLKDYINRFSNEALQVEGYDDGVALAAVI 204
                                      G  ESL+ +I RF+ +AL V+  DD + LAA  
Sbjct: 216 LSKLFVNNFIGGQRHKRSSSSLLTIEQGENESLRSFITRFNRDALAVDEVDDKLLLAAFH 275

Query: 205 SG------------RKRESEGGNINRHREDRGKRHQVEGRGQSRLEHSSAN--------- 264
           +G            +  ++    ++  +        +  + + RLE   AN         
Sbjct: 276 NGVHSDLFIHKLYEQAPQTMAELVHSAQNFMNAEDAIIAKKRKRLERVEANPMRQSEQGP 335

Query: 265 --GRGR-PEAKELQGRAEPRARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRN 324
              +GR  + K+   +A P AR   YTPL   LEQVL+ I+D   LK PEK+R DP++RN
Sbjct: 336 RPKKGRTKDRKDRDKKAGPSARNQQYTPLNMPLEQVLMQIKDEPSLKWPEKMRGDPNKRN 395

Query: 325 RNKYCMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPPL 384
           R+KYC FH DHGH T EC  LK +IE LIR+G LK F+G D     L     +    P  
Sbjct: 396 RSKYCRFHRDHGHDTDECFDLKQQIENLIRQGKLKNFLGRDHKDEKLKGKIEESSRPPLG 455

Query: 385 EIRTILGGPSGGESGRKRKAAIREAQ------QGPR------------EQEAVGIRHPHN 444
           EIR I+GG S G+S   +KA ++E Q      + PR            ++EA  I HPH+
Sbjct: 456 EIRVIIGGSSAGQSSETKKAYLKEVQSVQLFGRSPRARSTDEQTITFTDKEAERIHHPHD 515

Query: 445 DALVVTLTIANAKVHRILVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVSP 483
           DA+V+ L IA+    R+LVD GSSAD+L   AF  +++G ++ R   + LVGF   KV P
Sbjct: 516 DAIVIALLIADYTTRRVLVDNGSSADILYYPAFQQMRVGRNQLRPVHSPLVGFGGMKVQP 575

BLAST of Lag0035521 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 3.5e-70
Identity = 169/428 (39.49%), Postives = 229/428 (53.50%), Query Frame = 0

Query: 85  IGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSIGA--- 144
           + Q+D   DP  HLDAY+ WMD +G +EA RCR F+ TL   AR WF ++ R SI +   
Sbjct: 198 VKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFSTTLNGSARIWFRQLKRGSISSFKS 257

Query: 145 -----------------------------PESLKDYINRFSNEALQVEGYDDGVALAAVI 204
                                         ESL+DY+ RF+ E LQVEG  D V+L A +
Sbjct: 258 LARAFVTQFVGGRCRSRPVAYLLTIKQRTTESLRDYVARFNEEKLQVEGLTDAVSLLAFM 317

Query: 205 SGRKRESEGGNINRHRED-------RGKRHQVEG------------RGQSRLEHSSANGR 264
           SG + E    +  +   +       R +R+   G            R   + E S    +
Sbjct: 318 SGVRDEHLSFSFGKRTPNTFSEALSRAQRYMSAGEFFYSKREPDGKRTDPKRERSGDKPQ 377

Query: 265 G-RPEAKELQGRAEPRARFDMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKY 324
           G R E ++   + +P  +F+ YTP T  +EQVL+ I+D  LLK PE++++   +R++ +Y
Sbjct: 378 GSRWEKRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRLLKWPERMKASSAKRSKGRY 437

Query: 325 CMFHGDHGHTTRECIQLKDEIEALIREGYLKEFVGNDRSKRPLPADQGKGGANPPLEIRT 384
           C+FH DHGH T++C  LK+E+E LIR GYLKE+V     + P     G+   +P  EIRT
Sbjct: 438 CLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYV-----EEPKATQNGESDKSPAREIRT 497

Query: 385 ILGGPSGGESGRKRKAAIREAQQGPR-------------------EQEAVGIRHPHNDAL 442
           I+GGP   ESGRKRKA +REA+                       E EA  + HPHNDAL
Sbjct: 498 IMGGPIERESGRKRKADVREARTSREQNEVYHAYTTNRPVTIEFSEDEATHLLHPHNDAL 557

BLAST of Lag0035521 vs. ExPASy TrEMBL
Match: A0A2N9FE79 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13364 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 7.0e-63
Identity = 163/455 (35.82%), Postives = 240/455 (52.75%), Query Frame = 0

Query: 84  LIGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSIGA-- 143
           L+  +DG KDP  +L+A++T M      E   CRAF L L   AR WF K+  +SIG+  
Sbjct: 188 LLENFDGTKDPFDYLEAFKTIMQLQAVPEEVMCRAFPLGLRGSARVWFNKLESESIGSFV 247

Query: 144 ------------------------------PESLKDYINRFSNEALQVEGYDDGVALAAV 203
                                          ESL+ +++RF+ EA++++   + V + A 
Sbjct: 248 QLSRAFIDHFIGSQRRGRPPTHLLSVKQMEGESLRTFVHRFNEEAMKIDRPKEDVTVTAF 307

Query: 204 ISGRKRESEGGNINRHREDRGKRHQVEGRGQSRLEHSSANGRGRPEAKELQGRAEPRARF 263
           ++    E+      + R++   R         + E S        E  E +    P  +F
Sbjct: 308 MAEDALEAMDDPPPKRRKEAEDR---------KPEPSKQKVPKFTETPERKRTTAPPVKF 367

Query: 264 DMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDHGHTTRECIQLKD 323
             +TPL   ++++L+ IQD   L+ P K+RSDP+ R +N YC FH DHGH T EC+ LK+
Sbjct: 368 SSFTPLNTPIDKLLLQIQDDPSLRWPGKIRSDPNSRPKNLYCRFHRDHGHLTEECVALKE 427

Query: 324 EIEALIREGYLKEFVGNDRSKRPL--PADQGKGGAN---PPLEIRTILGGP-SGGESGRK 383
           +IE LIR+G L+++V    + RP   PA + +   N   P  EIRTI+GGP SGG S   
Sbjct: 428 QIETLIRQGKLQKYVSRPTNTRPAKPPAQREQAEHNRPGPVGEIRTIVGGPASGGTSRAS 487

Query: 384 RKAAIRE------AQQGPR------------EQEAVGIRHPHNDALVVTLTIANAKVHRI 443
           RKA  R+       Q+ P+            E++A G   PH+DALV+T+ IA     R+
Sbjct: 488 RKAYARQVHNIMVVQRPPKNIRLDDQIISFSEEDARGTHQPHDDALVITINIAGFTTRRV 547

Query: 444 LVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVSPRGNVELSVTFGEGPQTV 483
           +VD GSSAD+L   A+  ++L  D+ R     LVGF  +KV P G V L +T G  P+TV
Sbjct: 548 MVDNGSSADILYLPAYQQMRLDKDKLRPMDAPLVGFTGDKVCPVGIVTLPITVGTHPKTV 607

BLAST of Lag0035521 vs. ExPASy TrEMBL
Match: A0A7N2N9G0 (Reverse transcriptase OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.2e-62
Identity = 189/566 (33.39%), Postives = 271/566 (47.88%), Query Frame = 0

Query: 5   GDDQTQKEAGPSRKKTRMNSPLKTAPCTYIGNNDRKKLEARAKFEVEQGQKRREPSKWLK 64
           G   +   + P R+ T + S  + AP   I      K +        +G+   +    + 
Sbjct: 432 GPQASNAPSKPERQNTSIPSLAEIAPPLVIAEMQAMKEQMEVMMNALKGRVSSDLDDLVN 491

Query: 65  EEDSYQTSQKRT---ENEDIEGLIGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFAL 124
             DS  T+   +    ++     I  YDG KDP  HL+ ++T M   G  +A  CRAF  
Sbjct: 492 RTDSPFTATVNSYPLPSKFRMPQIDSYDGVKDPLDHLETFKTLMHLQGVADAIMCRAFPT 551

Query: 125 TLIDLARQWFGKISRKSIGA--------------------------------PESLKDYI 184
           TL   AR WF +++  SIG                                  E+L+ YI
Sbjct: 552 TLKGAARIWFSRLAPNSIGTFKELSAQFTAHFIGGHRYKKSTACLMSMKQREDETLRAYI 611

Query: 185 NRFSNEALQVEGYDDGVALAAVISGRKR--------------------------ESEGGN 244
           +RF+ EAL V+  DD + +AA  +G K+                           +E   
Sbjct: 612 SRFNKEALSVDEADDKILVAAFTNGLKKGKFLFSLYKNDPKTMSEVLYRATKYMNAEDAL 671

Query: 245 INRHREDRGKRHQVEGRGQSRLEHSSANGRGRPEAKELQGRAEPR---ARFDMYTPLTAS 304
           + R  + R +  Q + R           GR +    + +    PR    RF  +TPLTA 
Sbjct: 672 LAREEKPRKRERQEDNR--------QDQGRKKTRTGDRREERRPRPMGGRFTSFTPLTAP 731

Query: 305 LEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDHGHTTRECIQLKDEIEALIREG 364
           ++QVL+ I+D   L  P KL+SDP++R+R+KYC FH DHGH T +C  LK +IEALIR+G
Sbjct: 732 VDQVLMQIKDEEALTFPGKLKSDPNKRSRDKYCRFHRDHGHDTADCYDLKQQIEALIRQG 791

Query: 365 YLKEFVGNDRSKRPLPADQGKGGAN----PPL-EIRTILGG-PSGGESGRKRKAAIREAQ 424
            L+ FV  +R+  P   DQ     N    PP+ +IR I+GG  + G S + RK  +R  Q
Sbjct: 792 KLQRFVSKERADPP-AQDQHPRRDNERPRPPIGDIRMIVGGTAAAGSSKKARKTYLRMVQ 851

Query: 425 --------------QGP----REQEAVGIRHPHNDALVVTLTIANAKVHRILVDGGSSAD 483
                         +GP     E++A    HPH+DALVV+L + +  +HR+LVD GSSAD
Sbjct: 852 NVQLTGVVPKIARREGPIIGFSEEDAQRPHHPHDDALVVSLRVGDYNMHRVLVDNGSSAD 911

BLAST of Lag0035521 vs. ExPASy TrEMBL
Match: A0A2N9IJR2 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS52580 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 5.9e-62
Identity = 161/455 (35.38%), Postives = 240/455 (52.75%), Query Frame = 0

Query: 84  LIGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAFALTLIDLARQWFGKISRKSIGA-- 143
           L+  +DG KDP  +L+A++T M      E   CRAF L L   AR WF K+  +SIG+  
Sbjct: 186 LLENFDGTKDPFDYLEAFKTIMQLQAVPEEVMCRAFPLGLRGSARVWFNKLESESIGSFV 245

Query: 144 ------------------------------PESLKDYINRFSNEALQVEGYDDGVALAAV 203
                                          ESL+ +++RF+ EA++++   + V + A 
Sbjct: 246 QLSRAFIDHFIGSQRRGRPPTHLLSVKQMEGESLRTFVHRFNGEAMKIDRPKEDVTVTAF 305

Query: 204 ISGRKRESEGGNINRHREDRGKRHQVEGRGQSRLEHSSANGRGRPEAKELQGRAEPRARF 263
           ++    E+      +      +R +VE R   + E S        E  E +    P  +F
Sbjct: 306 MAEDALEAMDDPPPK------RRKEVEDR---KPEPSKQKVPKFTETPERKRTTAPPVKF 365

Query: 264 DMYTPLTASLEQVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDHGHTTRECIQLKD 323
             +TPL   ++++L+ IQD   L+ P K+RSDP+ R +N YC FH DHGH T EC+ LK+
Sbjct: 366 SSFTPLNTPIDKLLLQIQDDPSLRWPGKIRSDPNSRPKNLYCRFHRDHGHLTEECVALKE 425

Query: 324 EIEALIREGYLKEFVGNDRSKRPL--PADQGKGGAN---PPLEIRTILGGP-SGGESGRK 383
           ++E LIR+G L+++V    + RP   P  + +   N   P  EIRTI+GGP SGG S   
Sbjct: 426 QVETLIRQGKLQKYVNRPTNTRPAKPPVQREQAEPNRPGPVGEIRTIVGGPASGGTSRAS 485

Query: 384 RKAAIRE------AQQGPR------------EQEAVGIRHPHNDALVVTLTIANAKVHRI 443
           RKA  R+       Q+ P+            E++A G   PH+DALV+T+ IA     R+
Sbjct: 486 RKAYARQVHNIMVVQRPPKNIRLDDQIISFSEEDARGTHQPHDDALVITINIAGFTTRRV 545

Query: 444 LVDGGSSADVLSTTAFDAIKLGSDRPRSSLTSLVGFDREKVSPRGNVELSVTFGEGPQTV 483
           +VD GSSAD+L   A+  ++L  D+ R     LVGF  +K+ P G V L +  G  P+TV
Sbjct: 546 MVDNGSSADILYLPAYQQMRLDKDKLRPMDAPLVGFTGDKICPVGIVTLPIMVGTHPKTV 605

BLAST of Lag0035521 vs. ExPASy TrEMBL
Match: A0A7N2N013 (Ribonuclease H OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 3.8e-61
Identity = 192/563 (34.10%), Postives = 273/563 (48.49%), Query Frame = 0

Query: 3   PPGDDQTQKEAGPSRKKTRMNSPLKTAPCTYIGNNDRKKLEARAKFEVEQGQKRREPSKW 62
           P G +   K   P R+   + SP  TAP + I      K +        +G+   +    
Sbjct: 74  PQGSNAPSK---PVRRDISIPSPTDTAPQSIIAEMQAMKEQMDVMMSALKGRVSSDLDDL 133

Query: 63  LKEEDSYQTSQKRT---ENEDIEGLIGQYDGKKDPKQHLDAYQTWMDFHGANEATRCRAF 122
           +   DS  T+   +    ++     +  YDG KDP  HL+ ++T M   G  +A  CRAF
Sbjct: 134 VNRTDSPFTAAVNSFPLPSKFRMPSMDSYDGVKDPLDHLETFKTLMHLQGVADAIMCRAF 193

Query: 123 ALTLIDLARQWFGKISRKSIGA--------------------------------PESLKD 182
             TL   AR WF +I+  SI                                   E+L+ 
Sbjct: 194 PTTLKGAARIWFSRIAPNSISTFKELSAQFTTHFIGGHRYKKSTACLMNIKQREEETLRA 253

Query: 183 YINRFSNEALQVEGYDDGVALAAVISGRKRESEGGN--INRHREDRGKRHQVEGRGQSRL 242
           YI+RF+ EAL ++  DD + +AA  +G K    GG    + ++ D     +V  R    +
Sbjct: 254 YISRFNKEALSIDEADDKILVAAFTNGLK----GGKFLFSLYKNDPKTMSEVLYRATKYM 313

Query: 243 EHSSA--------NGRGRPE-AKELQGRAEPR--------------ARFDMYTPLTASLE 302
               A          R RPE +++ QGR + R               RF  +TPL A ++
Sbjct: 314 NAEDALLSREDRPKKRDRPEDSRQDQGRKKGRMGDRREDRRPKPMSGRFANFTPLNAPID 373

Query: 303 QVLVAIQDTNLLKRPEKLRSDPDRRNRNKYCMFHGDHGHTTRECIQLKDEIEALIREGYL 362
           QVL+ I+D   L  P KL+SDP +R+R+KYC FH DHGH T +C  LK +IEALIR+G L
Sbjct: 374 QVLMQIKDEGSLTFPGKLKSDPSKRSRDKYCRFHRDHGHDTADCYDLKQQIEALIRQGKL 433

Query: 363 KEFVGNDRSKRPLPADQGKGGAN----PPL-EIRTILGG-PSGGESGRKRKAAIREAQQ- 422
           ++FV   R+ +P P +Q     N    PP+ +IR I+GG  + G S + RK  +R  Q  
Sbjct: 434 QKFVNKGRTDQP-PQEQHLRRENERPRPPIGDIRMIVGGTAAAGSSKKARKTYLRMVQNV 493

Query: 423 -----------------GPREQEAVGIRHPHNDALVVTLTIANAKVHRILVDGGSSADVL 482
                            G  E++A  + HPH+DALVV++   +  VHR+L+D GSSAD+L
Sbjct: 494 QLTSRVPRIANGEEPIVGFTEEDARRLHHPHDDALVVSVRTGDYNVHRVLIDNGSSADIL 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030955724.11.4e-7038.39uncharacterized protein LOC115977839 [Quercus lobata][more]
XP_022158830.19.3e-7039.25uncharacterized protein LOC111025293 [Momordica charantia][more]
XP_030930616.12.1e-6938.27uncharacterized protein LOC115956348 [Quercus lobata][more]
XP_024041095.14.6e-6935.29uncharacterized protein LOC112098853 [Citrus clementina][more]
XP_023916366.11.3e-6637.08uncharacterized protein LOC112027956 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DWY03.5e-7039.49uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A2N9FE797.0e-6335.82Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13364 PE=4 SV=1[more]
A0A7N2N9G01.2e-6233.39Reverse transcriptase OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A2N9IJR25.9e-6235.38Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS52580 PE=4 SV=1[more]
A0A7N2N0133.8e-6134.10Ribonuclease H OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 359..479
e-value: 3.5E-8
score: 35.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..229
NoneNo IPR availablePANTHERPTHR33240:SF7GAG-PRO-LIKE PROTEINcoord: 372..482
NoneNo IPR availablePANTHERPTHR33240OS08G0508500 PROTEINcoord: 372..482
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 375..472
e-value: 1.97026E-12
score: 61.1984

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035521.1Lag0035521.1mRNA