Lag0035304 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035304
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA polymerase II transcriptional coactivator KELP-like
Locationchr3: 18372858 .. 18373647 (-)
RNA-Seq ExpressionLag0035304
SyntenyLag0035304
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCTTCCCTCTTCTCCTTCAATTCAGTTTCCTCCTTTGATTTTCCTTTCTTCCTCATCTCCTTCATCATGTTGTATTCTTGCCCTTCGTGTTCTCTGTTTTTTTTTTATCTTTTCTTCTCACACAATGCTCTTTCTTCGGTCAGGAAAGATATTGCTGAGCCCAACACATTCTTAGAACGAAAAACTTTCCCTAAAATTTCTTCAACTTCTGGACATTTCTACTGAATTCACTCTCTCTACCCGAAATCCACAGTGTTAATCGGAGAATTTCGATATGGATTCCGAAATCCAACGCAAAATCGAGGAAGTCATCCGCAGAATTCTCAAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTTCTAATCCCCTTACAAGGCTTTGTTCGCCAGGTTGTGGAATCCTTCCTCCATGAACGATCTGAAGAACAGCCACAAGAGGACGCCGGAGACGCCCTTGCTGCAAAGGAGAAGGAGTACGACGGCGACGACGACGATCTCGTTGTTTGTTGGTTTTTTGTTTTGCCTCTTCTCTTTCTAGTAGCTTCCGATTGCGGTTTCTTCCATTTGTAGTGAATACGTAGTGATTCTAGGCTCTGACTCTATGTTTGTGGTGTTCTTTGGCTCAGTTATCGAGCAAGAGGAAGGTGACTATTCAGGATTTTAAAGGGAAAACTCTGGTTTCGATTAGGGAGTTCATTAGAAAGGATGAGAAAGACCTCCCAACGTCAAAAGGTTTTGTCTAA

mRNA sequence

ATGCATCTTCCCTCTTCTCCTTCAATTCAGTTTCCTCCTTTGATTTTCCTTTCTTCCTCATCTCCTTCATCATGTTGTATTCTTGCCCTTCGTGTTCTCTTGTTAATCGGAGAATTTCGATATGGATTCCGAAATCCAACGCAAAATCGAGGAAGTCATCCGCAGAATTCTCAAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTTCTAATCCCCTTACAAGGCTTTGTTCGCCAGGTTGTGGAATCCTTCCTCCATGAACGATCTGAAGAACAGCCACAAGAGGACGCCGGAGACGCCCTTGCTGCAAAGGAGAAGGAGTACGACGGCGACGACGACGATCTCGTTTTATCGAGCAAGAGGAAGGTGACTATTCAGGATTTTAAAGGGAAAACTCTGGTTTCGATTAGGGAGTTCATTAGAAAGGATGAGAAAGACCTCCCAACGTCAAAAGGTTTTGTCTAA

Coding sequence (CDS)

ATGCATCTTCCCTCTTCTCCTTCAATTCAGTTTCCTCCTTTGATTTTCCTTTCTTCCTCATCTCCTTCATCATGTTGTATTCTTGCCCTTCGTGTTCTCTTGTTAATCGGAGAATTTCGATATGGATTCCGAAATCCAACGCAAAATCGAGGAAGTCATCCGCAGAATTCTCAAATCCTCCGACATGGACCAAATGACCGAGTCCAAGATTCGAGCCCTCGCCTCCAAGGACCTCGATCTCGACCTCTTCTAATCCCCTTACAAGGCTTTGTTCGCCAGGTTGTGGAATCCTTCCTCCATGAACGATCTGAAGAACAGCCACAAGAGGACGCCGGAGACGCCCTTGCTGCAAAGGAGAAGGAGTACGACGGCGACGACGACGATCTCGTTTTATCGAGCAAGAGGAAGGTGACTATTCAGGATTTTAAAGGGAAAACTCTGGTTTCGATTAGGGAGTTCATTAGAAAGGATGAGAAAGACCTCCCAACGTCAAAAGGTTTTGTCTAA

Protein sequence

MHLPSSPSIQFPPLIFLSSSSPSSCCILALRVLLLIGEFRYGFRNPTQNRGSHPQNSQILRHGPNDRVQDSSPRLQGPRSRPLLIPLQGFVRQVVESFLHERSEEQPQEDAGDALAAKEKEYDGDDDDLVLSSKRKVTIQDFKGKTLVSIREFIRKDEKDLPTSKGFV
Homology
[Close]
BLAST of Lag0035304 vs. NCBI nr
Match: XP_022931551.1 (RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP_023551821.1 RNA polymerase II transcriptional coactivator KELP-like [Cucurbita pepo subsp. pepo] >KAG6577134.1 RNA polymerase II transcriptional coactivator KELP, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015130.1 RNA polymerase II transcriptional coactivator KELP [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 115.2 bits (287), Expect = 5.6e-22
Identity = 69/114 (60.53%), Postives = 81/114 (71.05%), Query Frame = 0 

Query: 58  QILRHGPNDRVQDSSPRLQGPRSRPLLI---PLQGFVRQVVESFLHERSEEQPQEDAGDA 117
           +IL     D + +S  R    +   L +   P +  VR+VVESFL ERSE+QPQE+A DA
Sbjct: 15  RILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQERSEDQPQEEAEDA 74

Query: 118 LAAKEKEYDGDDDDLV--LSSKRKVTIQDFKGKTLVSIREFIRKDEKDLPTSKG 167
            AAKEKEYD D D +V  LSSKRKVTIQDF+GKTLVSIREF RKD KDLPT+KG
Sbjct: 75  SAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDGKDLPTAKG 128

[Close]
BLAST of Lag0035304 vs. NCBI nr
Match: XP_038878439.1 (RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida])

HSP 1 Score: 114.8 bits (286), Expect = 7.3e-22
Identity = 69/114 (60.53%), Postives = 80/114 (70.18%), Query Frame = 0 

Query: 58  QILRHGPNDRVQDSSPRLQGPRSRPLLI---PLQGFVRQVVESFLHERSEEQPQEDAGDA 117
           +IL     D + +S  R    +   L +   P +  VR+VVESFL ERSEEQPQ+ A DA
Sbjct: 15  RILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQERSEEQPQDQADDA 74

Query: 118 LAAKEKEYDGDDDDLV--LSSKRKVTIQDFKGKTLVSIREFIRKDEKDLPTSKG 167
            AAKEKEYD D D +V  LSSKRKVTIQDF+GKTLVSIREF RKD KDLPT+KG
Sbjct: 75  SAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDGKDLPTAKG 128

[Close]
BLAST of Lag0035304 vs. NCBI nr
Match: XP_008451928.1 (PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KAA0044928.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa] >TYK16542.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa])

HSP 1 Score: 112.1 bits (279), Expect = 4.8e-21
Identity = 68/114 (59.65%), Postives = 79/114 (69.30%), Query Frame = 0 

Query: 58  QILRHGPNDRVQDSSPRLQGPRSRPLLI---PLQGFVRQVVESFLHERSEEQPQEDAGDA 117
           +IL     D + +S  R    +   L +   P +  VR VVESFL ERSE+QPQ+ A DA
Sbjct: 15  RILETSDMDEMTESKIRALASKDLDLDLSKSPYKSLVRTVVESFLQERSEQQPQDQAEDA 74

Query: 118 LAAKEKEYDGDDDDLV--LSSKRKVTIQDFKGKTLVSIREFIRKDEKDLPTSKG 167
            AAKEKEYD D D +V  LSSKRKVTIQDF+GKTLVSIREF RKD KDLPT+KG
Sbjct: 75  SAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDGKDLPTAKG 128

[Close]
BLAST of Lag0035304 vs. NCBI nr
Match: XP_022985429.1 (RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 109.8 bits (273), Expect = 2.4e-20
Identity = 67/114 (58.77%), Postives = 80/114 (70.18%), Query Frame = 0 

Query: 58  QILRHGPNDRVQDSSPRLQGPRSRPLLI---PLQGFVRQVVESFLHERSEEQPQEDAGDA 117
           +IL     D + +S  R    +   L +   P +  VR+VVESFL +RSE+Q QE+A DA
Sbjct: 15  RILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQKRSEDQLQEEAEDA 74

Query: 118 LAAKEKEYDGDDDDLV--LSSKRKVTIQDFKGKTLVSIREFIRKDEKDLPTSKG 167
            AAKEKEYD D D +V  LSSKRKVTIQDF+GKTLVSIREF RKD KDLPT+KG
Sbjct: 75  SAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDGKDLPTAKG 128

[Close]
BLAST of Lag0035304 vs. NCBI nr
Match: XP_004148989.1 (RNA polymerase II transcriptional coactivator KELP [Cucumis sativus] >KGN53421.1 hypothetical protein Csa_014494 [Cucumis sativus])

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-19
Identity = 59/83 (71.08%), Postives = 66/83 (79.52%), Query Frame = 0 

Query: 86  PLQGFVRQVVESFLHERSEEQPQEDAGDALAAKEKEYDGDDDDLV--LSSKRKVTIQDFK 145
           P +  VR VVESFL ERSE+ P++   DA AAKEKEYD D D +V  LSSKRKVTIQDF+
Sbjct: 46  PYKSLVRTVVESFLQERSEQHPEDQPEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFR 105

Query: 146 GKTLVSIREFIRKDEKDLPTSKG 167
           GKTLVSIREF RKD KDLPT+KG
Sbjct: 106 GKTLVSIREFYRKDGKDLPTAKG 128

[Close]
BLAST of Lag0035304 vs. ExPASy Swiss-Prot
Match: O65155 (RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=3702 GN=KELP PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.5e-13
Identity = 46/89 (51.69%), Postives = 60/89 (67.42%), Query Frame = 0 

Query: 88  QGFVRQVVESFL-------HERSEEQPQEDAGDALAAK-EKEYDGDDDDLV--LSSKRKV 147
           + FVR VVE FL       +E S+   +E+ GD    K  KE+D D D ++  LS KR+V
Sbjct: 48  KAFVRSVVEKFLDEERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICRLSDKRRV 107

Query: 148 TIQDFKGKTLVSIREFIRKDEKDLPTSKG 167
           TIQ+FKGK+LVSIRE+ +KD K+LPTSKG
Sbjct: 108 TIQEFKGKSLVSIREYYKKDGKELPTSKG 136

The following BLAST results are available for this feature:
BLAST of Lag0035304 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022931551.15.6e-2260.53RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP... [more]
XP_038878439.17.3e-2260.53RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida][more]
XP_008451928.14.8e-2159.65PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KA... [more]
XP_022985429.12.4e-2058.77RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita ma... [more]
XP_004148989.11.5e-1971.08RNA polymerase II transcriptional coactivator KELP [Cucumis sativus] >KGN53421.1... [more]
BLAST of Lag0035304 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O651558.5e-1351.69RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=37... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003173Transcriptional coactivator p15 (PC4), C-terminalPFAMPF02229PC4coord: 131..166
e-value: 3.4E-11
score: 42.6
IPR009044ssDNA-binding transcriptional regulatorGENE3D2.30.31.10Transcriptional Coactivator Pc4; Chain Acoord: 112..168
e-value: 2.3E-11
score: 45.1
IPR009044ssDNA-binding transcriptional regulatorSUPERFAMILY54447ssDNA-binding transcriptional regulator domaincoord: 131..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 100..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..81
NoneNo IPR availablePANTHERPTHR13215:SF6RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR KELPcoord: 88..166
IPR045125RNA polymerase II transcriptional coactivator Sub1/Tcp4-likePANTHERPTHR13215RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATORcoord: 88..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035304.1Lag0035304.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription regulator complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003713 transcription coactivator activity