Lag0035271 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035271
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr3: 17839025 .. 17840773 (+)
RNA-Seq Expression	Lag0035271
Synteny	Lag0035271

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACTTGTTCTCCAATGCCAAAGCCCTTTCTTCTTTCTTCTTATCTCTTCTTTGGTTGCTTTTTGCAAACATACAGCTAACAAGTTAGTCTCTCTCTCACACATGAATGTTTCTACTTTATGCAATGCATCTACATTATGCATATAACAATCTGCATCCCATTTGATTCATATTTTTAGGTTTATTTTTCTATTTTTCTTTTTTTTCATGTTTTTTTTTTTTTGAGTTCAACAACATTTGAGTGTGGGGGATTTGAACTCCTAACTTCTGAGTCAAATGTATATGTCAGTTGGCCACTGACATATATGTTCATTTTGACTTGTTTTTTTCTTTATATTAAATCATAAAATAATAGTCAATGTAATTTGAATATGTATGTTAGAATTTTCTTTCGTTAAACTTTGACGAGTTTCAATTTTTAGCTTTAAACTTTGGACTACATGTTTTTTTTTGACATCATCCATAAATTATAGACATTAGCATGTGCCTACTAATTATTTTGTCCACTATTATTTGGCCCTATATCAATGCAATTATAGCAAGATTTATTTAACGATTTGAATTTTATTATGTCGTAACGACTTGTTTTTGTAGTTTATAAACACATTTGGTCCCTGATCAGTTTATATCAACCTACATATGTAAGAAATCTCATTAAATCTTATAAGGATGAAATTGTTACCTATCAAAGTTTAAGGCTAAGTTATTACAAACCGAAATATATATATATAAATCTCATCAAGCCATTAAAAAATTTATGTCTATGATTAAGCAAATTTTTATTGAATTTCATGCAGGTGCTCAAATTGGTGTGTGTTATGGACAACTTGGCAATGACCTACCATCTCATGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACTGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAAGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTACATCGATAACACTGCAGAAATCTCTCTCGATTATGCACTTTTTAGAGGAACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGAGACCGAGGAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACGACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATATCATGTCAACTTCAACTGA

mRNA sequence

ATGGCCAACTTGTTCTCCAATGCCAAAGCCCTTTCTTCTTTCTTCTTATCTCTTCTTTGGTTGCTTTTTGCAAACATACAGCTAACAAGTGCTCAAATTGGTGTGTGTTATGGACAACTTGGCAATGACCTACCATCTCATGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACTGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAAGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTACATCGATAACACTGCAGAAATCTCTCTCGATTATGCACTTTTTAGAGGAACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGAGACCGAGGAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACGACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATATCATGTCAACTTCAACTGA

Coding sequence (CDS)

ATGGCCAACTTGTTCTCCAATGCCAAAGCCCTTTCTTCTTTCTTCTTATCTCTTCTTTGGTTGCTTTTTGCAAACATACAGCTAACAAGTGCTCAAATTGGTGTGTGTTATGGACAACTTGGCAATGACCTACCATCTCATGCAGAAGTTATCGACCTTTACAAAAAAAACAACATCCAAAGAATGCGACTTTACGCTCCAACCCATCCTACTTTCAATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTCGGCATTCCCAACCATGAGCTCAAAGACATGGCCACCATCCAAGGAAAAGCTCATTCATGGGTCCAACACAACATCCTCAACTTTGGTGATGTCAAATTCAAGTACATTGTTGTCGGAAACGAAATCAAAACTTACGATCATGCTGCTCAATTCCTCGTACCAGCCATGCAAAACATCCAAAATGCAATTTCAGCCGTGGGCTTGAGAGGTAAAATCAAAGTTTCAACTTCATTCGACACTGGAATTCTTGCAGAGTCCTTCCCTCCTTCAAAGGGCTCCTTCAGAAGAGACTATCTTCCAATTCTTGACCCGACCATTCGCTTCCTCGTCGACAACAACTCGCCGTTGCTCGTCAACGTGTACCCTTACTTCAGCTACATCGATAACACTGCAGAAATCTCTCTCGATTATGCACTTTTTAGAGGAACGCCGGTTGTTGCAGATGACCAACTTGTTTATCGCAATTTGTTTGATGCCATTTTGGATGCTGTTTACTCTGCTTTGGAGAAGTCTGGCGGTGGAGGTCTGGAAATTGTTGTATCGGAGACTGGTTGGCCAACAGATCGTGGGGTGGCGGCGAACGTAGATAATGCAAGGACTTACATCAACAATCTAATTCAACATGTGAAAGTAGGGACACCGAAGAGACCGAGGAGGCCTGTTGAGACCTACGTGTTTGCGATGTTCGATGAGAATGAGAAAACGACTCCTCCTGAAGTTGAGAAACATTGGGGTTTGTTCTCTCCAAACAAGCAACCCAAATATCATGTCAACTTCAACTGA

Protein sequence

MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN

Homology

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BLAST of Lag0035271 vs. NCBI nr
Match: XP_022961079.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 567.8 bits (1462), Expect = 6.5e-158
Identity = 280/345 (81.16%), Postives = 308/345 (89.28%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQ 60
           MA  FSNA  LS+FFLSLLWLLFA++ LTSAQIGVCYGQLGNDLPS  EVI+LYK+NNIQ
Sbjct: 1   MAIFFSNANGLSTFFLSLLWLLFASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQ 60

Query: 61  RMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYI 120
           RMRLYA  HPTF+ALRGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYI
Sbjct: 61  RMRLYALNHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYI 120

Query: 121 VVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRR 180
           VVGNEIKTYD AA FLVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ 
Sbjct: 121 VVGNEIKTYDQAASFLVPAMQNIQNAISAVGLEGQIKVSTAFDTGILAESYPPSKGSFKT 180

Query: 181 DYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRN 240
           DYLPIL+PTIRFL+DNN+PLLVNVYPYFSY+ NTA+IS+DYALFRG +P V D Q VY N
Sbjct: 181 DYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEK+GG GLEIVVSETGWPT+ G AA V+NA TY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKAGGDGLEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           R  +PVETYVFAMFDENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 301 RSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

[Close]

BLAST of Lag0035271 vs. NCBI nr
Match: XP_022987103.1 (probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima])

HSP 1 Score: 562.8 bits (1449), Expect = 2.1e-156
Identity = 278/345 (80.58%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQ 60
           MA  FSNA  LS+FF+SLLWLLFA + LTSAQIGVCYGQLGNDLPS  EVI+LYK+NNIQ
Sbjct: 1   MAIYFSNANGLSTFFVSLLWLLFARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQ 60

Query: 61  RMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYI 120
           RMRLYAP HPTF+ALRGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYI
Sbjct: 61  RMRLYAPDHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYI 120

Query: 121 VVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRR 180
           VVGNEIKT D AA FLVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ 
Sbjct: 121 VVGNEIKTNDQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKT 180

Query: 181 DYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGT-PVVADDQLVYRN 240
           DYLPIL+PTIRFL+DNN+PLLVNVYPYFSY+ NTA+IS+DYALFRGT P V D Q VY N
Sbjct: 181 DYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEK+G  GLEI VSETGWPT+ G AA V+NA TY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKAGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           R  +PVETYVFAMFDENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 301 RSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

[Close]

BLAST of Lag0035271 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 555.1 bits (1429), Expect = 4.4e-154
Identity = 275/345 (79.71%), Postives = 302/345 (87.54%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLW-LLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNI 60
           MAN ++NAK  S FF  LLW LL AN+ LT+AQIGVCYGQLG+DLPS  EVIDLY +NNI
Sbjct: 1   MANFYANAKGFSIFFSFLLWLLLIANLHLTNAQIGVCYGQLGDDLPSPVEVIDLYNQNNI 60

Query: 61  QRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKY 120
           +RMRLYAP  P+ NALRGSNIELMLGIPNHEL+ MAT Q  A+SW Q N+L+FGDVKFKY
Sbjct: 61  ERMRLYAPNQPSLNALRGSNIELMLGIPNHELEAMATTQENANSWFQDNVLSFGDVKFKY 120

Query: 121 IVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFR 180
           IVVGNEIK  D AA+FLVPAMQNIQNAISA GLRGKIKVST+ DTG+LAES+PPSKGSFR
Sbjct: 121 IVVGNEIKPSDGAARFLVPAMQNIQNAISAAGLRGKIKVSTAIDTGVLAESYPPSKGSFR 180

Query: 181 RDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVYRN 240
            DYLPILDPTIRFLVDNNSPLL+N+Y YFS+I NT +ISLDYALF G  +V+D QLVY+N
Sbjct: 181 SDYLPILDPTIRFLVDNNSPLLLNLYTYFSHISNTKDISLDYALFTGMSMVSDGQLVYQN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEKS GGGLEIVVSE+GWPTD G AA VDNARTY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKSAGGGLEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           RP  P+ETYVFAMFDEN+KTTPPEVEKHWGLFSPNKQPKY VNF+
Sbjct: 301 RPGMPIETYVFAMFDENQKTTPPEVEKHWGLFSPNKQPKYLVNFH 345

[Close]

BLAST of Lag0035271 vs. NCBI nr
Match: KAG6590085.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.3 bits (1370), Expect = 3.0e-147
Identity = 261/322 (81.06%), Postives = 285/322 (88.51%), Query Frame = 0


Query: 24  ANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELM 83
           AN     AQIGVCYGQLGNDLPS  EVI+LYK+NNIQRMRLYAP HPTF+ALRGSNIELM
Sbjct: 71  ANNSSIGAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPNHPTFDALRGSNIELM 130

Query: 84  LGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNI 143
           LGIPN+EL+D+A  Q KA SWVQ N+++FGDVKFKYIVVGNEIKTYD AA FLVPAMQNI
Sbjct: 131 LGIPNNELEDLANFQEKADSWVQDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNI 190

Query: 144 QNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRFLVDNNSPLLVN 203
           QNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ DYLPIL+PTIRFL+DNN+PLLVN
Sbjct: 191 QNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVN 250

Query: 204 VYPYFSYIDNTAEISLDYALFRGT-PVVADDQLVYRNLFDAILDAVYSALEKSGGGGLEI 263
           VYPYFSY+ NTA+IS+DYALFRGT P V D Q VY NLFDAILDAVYSALE SGG  +EI
Sbjct: 251 VYPYFSYVANTADISMDYALFRGTSPSVVDGQFVYHNLFDAILDAVYSALETSGGDRVEI 310

Query: 264 VVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPP 323
           VVSETGWPT+ G AA V+NA TY NNLIQHVK GTPKR  +PVETYVFAMFDENEKTTPP
Sbjct: 311 VVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPP 370

Query: 324 EVEKHWGLFSPNKQPKYHVNFN 345
           EVEKHWGLFSP+K PKY VNFN
Sbjct: 371 EVEKHWGLFSPSKLPKYEVNFN 392

[Close]

BLAST of Lag0035271 vs. NCBI nr
Match: XP_038895085.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 4.4e-146
Identity = 265/346 (76.59%), Postives = 299/346 (86.42%), Query Frame = 0


Query: 1   MANLF-SNAKALSSF-FLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNN 60
           MANLF SNAKALS+  FLSLL L  AN+Q+  A+IGVCYGQLGNDLPS  EVI+LYK+NN
Sbjct: 1   MANLFHSNAKALSTLTFLSLLSLQLANLQIAGAEIGVCYGQLGNDLPSPQEVIELYKQNN 60

Query: 61  IQRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFK 120
           IQRMRLYAP  PTF+ALRGSNIELMLGIPN +++ MA  Q  A++WVQ NILNF DVKFK
Sbjct: 61  IQRMRLYAPNQPTFDALRGSNIELMLGIPNDQIRSMAATQDNANAWVQENILNFPDVKFK 120

Query: 121 YIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSF 180
           YIVVGNEIKT + AA FLVPAMQNIQNAISAVGL+G+IKVST+FDTGIL++SFPPS+GSF
Sbjct: 121 YIVVGNEIKTNEEAATFLVPAMQNIQNAISAVGLQGQIKVSTAFDTGILSQSFPPSQGSF 180

Query: 181 RRDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVYR 240
             +YLPIL+PT RFL+DN+SPLLVN+YPYFSY+  T  + LDYA+F GT ++ D +L Y+
Sbjct: 181 DTNYLPILNPTFRFLLDNSSPLLVNLYPYFSYV-TTPRMGLDYAIFTGTSLIGDGELNYQ 240

Query: 241 NLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTP 300
           NLFDAILDAVYSALEKSGGG LEIVVSETGWPTD G AA VDNARTY NNLIQHV  GTP
Sbjct: 241 NLFDAILDAVYSALEKSGGGSLEIVVSETGWPTDHGDAATVDNARTYNNNLIQHVNQGTP 300

Query: 301 KRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           KR  R +ETY+FAMFDENEKTTPPEVE+HWGLFSPNKQPKY VNFN
Sbjct: 301 KRRGRAIETYLFAMFDENEKTTPPEVERHWGLFSPNKQPKYSVNFN 345

[Close]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.2e-116
Identity = 204/322 (63.35%), Postives = 246/322 (76.40%), Query Frame = 0


Query: 24  ANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNALRGSNIELM 83
           A  Q T A IGVC G +G+DLP  AEV+ LYK NNI RMRLY P      ALRGSNI+L+
Sbjct: 31  ATKQHTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLL 90

Query: 84  LGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQFLVPAMQNI 143
           LG+PN  L+ +A  Q  A++WVQ+N+ N+ +VKFKYI VGNE+K  D  AQFLVPAM+NI
Sbjct: 91  LGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNI 150

Query: 144 QNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRFLVDNNSPLLVN 203
           Q AIS  GL  KIKVST+ DTG+L E+FPPS GSF+ +Y  +L P IRFLV + SPLLVN
Sbjct: 151 QEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVN 210

Query: 204 VYPYFSYIDNTAEISLDYALFRGTPVVADD-QLVYRNLFDAILDAVYSALEKSGGGGLEI 263
           +YPYF+Y  NT +I LDYALF    VV  D    YRNLFDA+LD VY+ALEK+GGG L++
Sbjct: 211 LYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKV 270

Query: 264 VVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDENEKTTPP 323
           V+SETGWP+  G A  +DNART+I+NLIQHVK GTP+RP RP+ETY+FAMFDEN KT  P
Sbjct: 271 VISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKT--P 330

Query: 324 EVEKHWGLFSPNKQPKYHVNFN 345
           E+EKHWGLFSP KQPKY ++FN
Sbjct: 331 ELEKHWGLFSPTKQPKYQISFN 350

[Close]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.0e-113
Identity = 199/328 (60.67%), Postives = 250/328 (76.22%), Query Frame = 0


Query: 18  LLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNALRG 77
           LL L F N   T  Q+GVCYG+ GN+LPS AE I L+K+ NIQR+RLY+P H    ALRG
Sbjct: 12  LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRG 71

Query: 78  SNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTYDHAAQFL 137
           SNIE+ LG+PN  L+ +A+ Q +A++WVQ  ++N+ + V+F+YI VGNE+K  D  AQFL
Sbjct: 72  SNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFL 131

Query: 138 VPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRFLVDN 197
           VPAM+NI  A+ A GL G+IKVSTS D G+L ES+PPSKGSFR D + +++P IRFLV  
Sbjct: 132 VPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSK 191

Query: 198 NSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEKS 257
           NSPLL+N+Y YFSY  N  +I LDYALF   + +V+D    Y+NLFDA+LDA+YSALEKS
Sbjct: 192 NSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKS 251

Query: 258 GGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDE 317
           GG  LEIVV+ETGWPT  G   N++NAR Y NNLI+HVK GTPKRP + +ETY+FA++DE
Sbjct: 252 GGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDE 311

Query: 318 NEKTTPPEVEKHWGLFSPNKQPKYHVNF 344
           N+K TPP VEK WGLF PNKQPKY +NF
Sbjct: 312 NQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.7e-108
Identity = 207/349 (59.31%), Postives = 256/349 (73.35%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLW-LLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNI 60
           MA   S+ K+ S   ++ L+ LL      T AQ GVCYG+LGN+LP+  EV+ LY + NI
Sbjct: 1   MAKYHSSGKSSSMTAIAFLFILLITYTGTTDAQSGVCYGRLGNNLPTPQEVVALYNQANI 60

Query: 61  QRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNF-GDVKFK 120
           +RMR+Y P+     ALRGSNIEL+L IPN  L+++A+ Q  A+ WVQ NI N+  +V+F+
Sbjct: 61  RRMRIYGPSPEVLEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFR 120

Query: 121 YIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSF 180
           Y+ VGNE+K     AQFLVPA++NIQ AIS  GL  ++KVST+ DTG LAESFPPSKGSF
Sbjct: 121 YVSVGNEVKPEHSFAQFLVPALENIQRAISNAGLGNQVKVSTAIDTGALAESFPPSKGSF 180

Query: 181 RRDYL-PILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADD-QLV 240
           + DY    LD  IRFLV+NN+PL+VNVY YF+Y  N  +ISLDYALFR   VV  D  L 
Sbjct: 181 KSDYRGAYLDGVIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLG 240

Query: 241 YRNLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVG 300
           YRNLFDA +DAVY+ALEK+GGG L IVVSE+GWP+  G A ++DNARTY  NL+++VK G
Sbjct: 241 YRNLFDASVDAVYAALEKAGGGSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQG 300

Query: 301 TPKRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSP-NKQPKYHVNFN 345
           TPKRP  P+ETYVFAMFDEN+K   PE EK WGLFSP  KQPKY +NFN
Sbjct: 301 TPKRPGAPLETYVFAMFDENQK--QPEFEKFWGLFSPITKQPKYSINFN 347

[Close]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 9.0e-102
Identity = 188/334 (56.29%), Postives = 243/334 (72.75%), Query Frame = 0


Query: 16  LSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNAL 75
           L L  L  A +  T AQ GVCYG+ GN LPS A+V+ L  +NNI+RMR+Y P  PT  AL
Sbjct: 9   LLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTLEAL 68

Query: 76  RGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYIVVGNEIKTYDHAAQF 135
           RGSNIELMLG+PN +L+++A  Q  A +WVQ+N+ N+G+VKF+YI VGNE+   +  +++
Sbjct: 69  RGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNENSKY 128

Query: 136 ---LVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRF 195
              L+ AM+NIQ AIS  GL  +IKVST+ +TG+  ++ PPS G F+ D    ++P I F
Sbjct: 129 VPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPIINF 188

Query: 196 LVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQ-LVYRNLFDAILDAVYSA 255
           LV N +PLLVN+YPYF+ I N A+I L+YALF  + VV +D    YRNLFDAILDA YSA
Sbjct: 189 LVTNRAPLLVNLYPYFA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYSA 248

Query: 256 LEKSGGGGLEIVVSETGWPT-DRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVF 315
           LEK+ G  LEIVVSE+GWP+   G   ++DNARTY NNLI HVK G+PKRP  P+ETYVF
Sbjct: 249 LEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIETYVF 308

Query: 316 AMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           A+FDE++K   PE+EKH+GLFS N QPKY ++FN
Sbjct: 309 ALFDEDQK--DPEIEKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 367.1 bits (941), Expect = 2.2e-100
Identity = 188/340 (55.29%), Postives = 242/340 (71.18%), Query Frame = 0


Query: 10  ALSSFFLSLLWLLFANIQLTSAQ-IGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPT 69
           AL    + LL LL ++ ++  AQ +GVCYG LGN+LP  ++V+ LYK  NI+RMRLY P 
Sbjct: 9   ALQMAAIILLGLLVSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPN 68

Query: 70  HPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIK 129
                ALRGSNIE+MLG+PN +L+++A     A++WVQ N+ NF   VKF+YI VGNE+ 
Sbjct: 69  QAALQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVS 128

Query: 130 TY---DHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLP 189
                    ++L+PAM+NI+NAIS+ GL+  IKVS+S D  ++  SFPPS+GSFR D   
Sbjct: 129 PVTGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRS 188

Query: 190 ILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADD-QLVYRNLFDA 249
            +DP I F+   NSPLLVN+YPYFSY  N  +ISL YALF    VV  D  L YRNLFDA
Sbjct: 189 FIDPIIGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDA 248

Query: 250 ILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRR 309
           + DAVY+AL ++GGG +EIVVSE+GWP+    AA  +NA TY  NLIQHVK G+P+RP +
Sbjct: 249 MSDAVYAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNK 308

Query: 310 PVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNF 344
            +ETY+FAMFDEN K   PE+EKH+GLFSPNKQPKY ++F
Sbjct: 309 VIETYLFAMFDENNKN--PELEKHFGLFSPNKQPKYPLSF 346

[Close]

BLAST of Lag0035271 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.1e-158
Identity = 280/345 (81.16%), Postives = 308/345 (89.28%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQ 60
           MA  FSNA  LS+FFLSLLWLLFA++ LTSAQIGVCYGQLGNDLPS  EVI+LYK+NNIQ
Sbjct: 1   MAIFFSNANGLSTFFLSLLWLLFASMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQ 60

Query: 61  RMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYI 120
           RMRLYA  HPTF+ALRGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYI
Sbjct: 61  RMRLYALNHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYI 120

Query: 121 VVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRR 180
           VVGNEIKTYD AA FLVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ 
Sbjct: 121 VVGNEIKTYDQAASFLVPAMQNIQNAISAVGLEGQIKVSTAFDTGILAESYPPSKGSFKT 180

Query: 181 DYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRN 240
           DYLPIL+PTIRFL+DNN+PLLVNVYPYFSY+ NTA+IS+DYALFRG +P V D Q VY N
Sbjct: 181 DYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGASPSVEDGQFVYHN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEK+GG GLEIVVSETGWPT+ G AA V+NA TY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKAGGDGLEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           R  +PVETYVFAMFDENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 301 RSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

[Close]

BLAST of Lag0035271 vs. ExPASy TrEMBL
Match: A0A6J1JIH6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.0e-156
Identity = 278/345 (80.58%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQ 60
           MA  FSNA  LS+FF+SLLWLLFA + LTSAQIGVCYGQLGNDLPS  EVI+LYK+NNIQ
Sbjct: 1   MAIYFSNANGLSTFFVSLLWLLFARMHLTSAQIGVCYGQLGNDLPSPVEVIELYKQNNIQ 60

Query: 61  RMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYI 120
           RMRLYAP HPTF+ALRGSNIELMLGIPN+EL+D+A  Q KA SWV  N+++FGDVKFKYI
Sbjct: 61  RMRLYAPDHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYI 120

Query: 121 VVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRR 180
           VVGNEIKT D AA FLVPAMQNIQNAISAVGL G+IKVST+FDTGILAES+PPSKGSF+ 
Sbjct: 121 VVGNEIKTNDQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKT 180

Query: 181 DYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGT-PVVADDQLVYRN 240
           DYLPIL+PTIRFL+DNN+PLLVNVYPYFSY+ NTA+IS+DYALFRGT P V D Q VY N
Sbjct: 181 DYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEK+G  GLEI VSETGWPT+ G AA V+NA TY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKAGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           R  +PVETYVFAMFDENEKTTPPEVEKHWGLFSP+K PKY VNFN
Sbjct: 301 RSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 345

[Close]

BLAST of Lag0035271 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.1e-154
Identity = 275/345 (79.71%), Postives = 302/345 (87.54%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLW-LLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNI 60
           MAN ++NAK  S FF  LLW LL AN+ LT+AQIGVCYGQLG+DLPS  EVIDLY +NNI
Sbjct: 1   MANFYANAKGFSIFFSFLLWLLLIANLHLTNAQIGVCYGQLGDDLPSPVEVIDLYNQNNI 60

Query: 61  QRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKY 120
           +RMRLYAP  P+ NALRGSNIELMLGIPNHEL+ MAT Q  A+SW Q N+L+FGDVKFKY
Sbjct: 61  ERMRLYAPNQPSLNALRGSNIELMLGIPNHELEAMATTQENANSWFQDNVLSFGDVKFKY 120

Query: 121 IVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFR 180
           IVVGNEIK  D AA+FLVPAMQNIQNAISA GLRGKIKVST+ DTG+LAES+PPSKGSFR
Sbjct: 121 IVVGNEIKPSDGAARFLVPAMQNIQNAISAAGLRGKIKVSTAIDTGVLAESYPPSKGSFR 180

Query: 181 RDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVYRN 240
            DYLPILDPTIRFLVDNNSPLL+N+Y YFS+I NT +ISLDYALF G  +V+D QLVY+N
Sbjct: 181 SDYLPILDPTIRFLVDNNSPLLLNLYTYFSHISNTKDISLDYALFTGMSMVSDGQLVYQN 240

Query: 241 LFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPK 300
           LFDAILDAVYSALEKS GGGLEIVVSE+GWPTD G AA VDNARTY NNLIQHVK GTPK
Sbjct: 241 LFDAILDAVYSALEKSAGGGLEIVVSESGWPTDGGEAAKVDNARTYNNNLIQHVKGGTPK 300

Query: 301 RPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           RP  P+ETYVFAMFDEN+KTTPPEVEKHWGLFSPNKQPKY VNF+
Sbjct: 301 RPGMPIETYVFAMFDENQKTTPPEVEKHWGLFSPNKQPKYLVNFH 345

[Close]

BLAST of Lag0035271 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.5e-139
Identity = 259/347 (74.64%), Postives = 289/347 (83.29%), Query Frame = 0


Query: 1   MANLF-SNAKALSSF-FLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNN 60
           MANLF S+ K LS+   +S+L LL  N+Q T AQIGVCYGQLGNDLPS AEVIDLY +NN
Sbjct: 1   MANLFPSSDKGLSTLTLISILCLLLKNLQTTVAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFK 120
           IQRMRLYAP   TFNALRGS+IELMLG+PN E++ MA  Q  A++WVQ NILNF DV FK
Sbjct: 61  IQRMRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFK 120

Query: 121 YIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILA-ESFPPSKGS 180
           YIVVGNEIKT + AA+FLVPAMQNIQNAISAVGL GKIKVST+F TGIL+ ESFPPS GS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGS 180

Query: 181 FRRDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVY 240
           F   YLPIL+PTIRFL+DNNSPLLVN+YPYFSY+  T  + LDYA+F GT +V D +  Y
Sbjct: 181 FDASYLPILNPTIRFLLDNNSPLLVNLYPYFSYV-GTPSMELDYAIFTGTSLVVDGEFNY 240

Query: 241 RNLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGT 300
           +NLF AILD VYSALEK+GGG LEIVVSETGWPTD G AA VDNARTY NNLIQ VK GT
Sbjct: 241 QNLFHAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGT 300

Query: 301 PKRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           PKR  R +ETYVFAMFDENEKTTPPEVE+HWGLFSPNKQPK+ V+FN
Sbjct: 301 PKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 346

[Close]

BLAST of Lag0035271 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 4.3e-139
Identity = 256/346 (73.99%), Postives = 291/346 (84.10%), Query Frame = 0


Query: 1   MANLF-SNAKALSSF-FLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNN 60
           MANLF SNAK LS+   +S+L LL  N+Q T AQIGVCYGQLGNDLPS AEVIDLY +NN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFK 120
           IQRMRLYAP   TFNALRGS+IELMLG+PN +++ MA  Q  A++W+Q NILNF DV FK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGIL-AESFPPSKGS 180
           YIVVGNEIKT + AA+FLVPAMQNIQNAISAVGL+G+IKVST+F TGIL AESFPPS GS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FRRDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQLVY 240
           F  +YLPIL+PTIRFL+DNNSPLL+N+YPYFSY+  T  + LDYA+F GT +V D +  Y
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYV-ATPNMELDYAIFTGTSLVEDGEFNY 240

Query: 241 RNLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGT 300
           +NLFDAILD VYSALEK+GGG LE+VVSETGWPT+ G AA VDNARTY NNLIQHVK GT
Sbjct: 241 QNLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGT 300

Query: 301 PKRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNF 344
           PKR  R +ETYVFAMFDENEKTTPPEVE+HWGLFSPNKQPK  + F
Sbjct: 301 PKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKSFLCF 345

[Close]

BLAST of Lag0035271 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 409.8 bits (1052), Expect = 2.1e-114
Identity = 199/328 (60.67%), Postives = 250/328 (76.22%), Query Frame = 0


Query: 18  LLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFNALRG 77
           LL L F N   T  Q+GVCYG+ GN+LPS AE I L+K+ NIQR+RLY+P H    ALRG
Sbjct: 12  LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRG 71

Query: 78  SNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGD-VKFKYIVVGNEIKTYDHAAQFL 137
           SNIE+ LG+PN  L+ +A+ Q +A++WVQ  ++N+ + V+F+YI VGNE+K  D  AQFL
Sbjct: 72  SNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFL 131

Query: 138 VPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTIRFLVDN 197
           VPAM+NI  A+ A GL G+IKVSTS D G+L ES+PPSKGSFR D + +++P IRFLV  
Sbjct: 132 VPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSK 191

Query: 198 NSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYRNLFDAILDAVYSALEKS 257
           NSPLL+N+Y YFSY  N  +I LDYALF   + +V+D    Y+NLFDA+LDA+YSALEKS
Sbjct: 192 NSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKS 251

Query: 258 GGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKRPRRPVETYVFAMFDE 317
           GG  LEIVV+ETGWPT  G   N++NAR Y NNLI+HVK GTPKRP + +ETY+FA++DE
Sbjct: 252 GGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDE 311

Query: 318 NEKTTPPEVEKHWGLFSPNKQPKYHVNF 344
           N+K TPP VEK WGLF PNKQPKY +NF
Sbjct: 312 NQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Lag0035271 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 360.9 bits (925), Expect = 1.1e-99
Identity = 192/346 (55.49%), Postives = 240/346 (69.36%), Query Frame = 0


Query: 1   MANLFSNAKALSSFFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQ 60
           M N  S   +L    L L ++L +       QIGVCYG+ GN+L   +EV+ LY++ NI+
Sbjct: 3   MCNGSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIR 62

Query: 61  RMRLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGDVKFKYI 120
           RMRLY P   T NALRGSNIEL+L +PN +L+ +A+ Q +A +WV++N+ N+ +V F+YI
Sbjct: 63  RMRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYI 122

Query: 121 VVGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDT-GILAESFPPSKGSFR 180
            VGNE++  D AA F++PAMQNI+ A+S++G    IKVST+ DT GI    FPPS G+F 
Sbjct: 123 SVGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGI--SGFPPSSGTFT 182

Query: 181 RDYLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRG-TPVVADDQLVYR 240
            ++   + P I FL    SPLLVN YPYFSY  N  +I LDY LF   + VV D Q  YR
Sbjct: 183 PEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYR 242

Query: 241 NLFDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTP 300
           NLF AILD VY++LEK+GGG LEIVVSE+GWPT  G A  VDNARTY+NNLIQ VK G+P
Sbjct: 243 NLFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSP 302

Query: 301 KRPRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           +RP R  ETY+FAMFDEN K   PE EK WGLF PN QPKY VNFN
Sbjct: 303 RRPGRATETYIFAMFDENSK-QGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of Lag0035271 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 355.1 bits (910), Expect = 6.2e-98
Identity = 187/344 (54.36%), Postives = 246/344 (71.51%), Query Frame = 0


Query: 6   SNAKALSS--FFLSLLWLLFAN-IQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRM 65
           S +++L+S    + LL L+ A+    T+ QIGVCYG LG+ LPS ++V+ LYK+ NIQRM
Sbjct: 2   SESRSLASPPMLMILLSLVIASFFNHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRM 61

Query: 66  RLYAPTHPTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILNFGD-VKFKYIV 125
           RLY P      ALRGS+IEL+L +P+ +L+ +A+ Q +A  WVQ N+ ++ D V+F+YI 
Sbjct: 62  RLYGPDPGALAALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYIN 121

Query: 126 VGNEIKTYDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRD 185
           VGNE+K       FL+ AMQNI+NA+S  GL  ++KVST+  T    ++ PPS+G FR +
Sbjct: 122 VGNEVK--PSVGGFLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDE 181

Query: 186 YLPILDPTIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQ-LVYRNL 245
           Y   L+P I FL    SPLLVN+YPYFSY+ +TA I LDYALF     V +D    Y+NL
Sbjct: 182 YKSFLEPVIGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNL 241

Query: 246 FDAILDAVYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKVGTPKR 305
           FDA LD+VY+ALEKSGGG LEIVVSETGWPT+  V  +V+NA+TY+NNLIQHVK G+P+R
Sbjct: 242 FDANLDSVYAALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRR 301

Query: 306 PRRPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           P + +ETY+FAMFDEN+K   P  EK WGLF P++Q KY VNFN
Sbjct: 302 PGKAIETYIFAMFDENKK--EPTYEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of Lag0035271 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.3e-91
Identity = 168/339 (49.56%), Postives = 234/339 (69.03%), Query Frame = 0


Query: 11  LSSFFLSLLWLLFANIQLTSAQ-IGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTH 70
           +++ FL +   +   +  TS + +GVCYG +GN+LPS ++ I L+++NNI+R+RLY P  
Sbjct: 1   MTTLFLLIALFITTILNPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQ 60

Query: 71  PTFNALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNILN-FGDVKFKYIVVGNEIKT 130
              NALR + IE+++G+PN +L+ + T    A SW+Q+N+LN +  V FKYI VGNE+  
Sbjct: 61  AALNALRNTGIEVIIGVPNTDLRSL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSP 120

Query: 131 YDHAAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDP 190
             +    ++PAM+N+ +A+    L+ +IKVST+ D  ++  SFPPS G FR D    +DP
Sbjct: 121 -SNGGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDP 180

Query: 191 TIRFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVA-DDQLVYRNLFDAILDA 250
            I FL   NS LL N+YPYFSY+DN  +ISL YALF    VV  D    Y+NLFDA+LD 
Sbjct: 181 VIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDV 240

Query: 251 VYSALEKSGGGGLEIVVSETGWPTDRGVAANVDNARTYINNLIQHVKV--GTPKRPRRPV 310
           VYSA+E+SGGG L +VVSE+GWP++ G AA+ DNAR +  NL   V+   GTPKRP R V
Sbjct: 241 VYSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGV 300

Query: 311 ETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNFN 345
           ETY+FAMFDEN+K+  PE+EK++GLF PNKQPK+ + F+
Sbjct: 301 ETYLFAMFDENQKS--PEIEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Lag0035271 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 134/341 (39.30%), Postives = 208/341 (61.00%), Query Frame = 0


Query: 14  FFLSLLWLLFANIQLTSAQIGVCYGQLGNDLPSHAEVIDLYKKNNIQRMRLYAPTHPTFN 73
           F +S+L LL         ++GVCYG+  +DLP+ ++V+ L +++NI+ +R+Y        
Sbjct: 9   FSISILLLLLDCCY--GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLK 68

Query: 74  ALRGSNIELMLGIPNHELKDMATIQGKAHSWVQHNIL-NFGDVKFKYIVVGNEIKTYDH- 133
           A   ++IELM+G+PN +L   +  Q    +W+++++L  +   K  YI VG E     H 
Sbjct: 69  AFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHI 128

Query: 134 -AAQFLVPAMQNIQNAISAVGLRGKIKVSTSFDTGILAESFPPSKGSFRRDYLPILDPTI 193
            A+ F+VPAMQN+  A+  VGL  +IKVST+   GIL+ SFPPS G+F   Y   L P +
Sbjct: 129 NASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPML 188

Query: 194 RFLVDNNSPLLVNVYPYFSYIDNTAEISLDYALFRGTPVVADDQ--LVYRNLFDAILDAV 253
            FL +N SP ++++YPY++Y D+   +SLDY LF  +  V D    L+Y+N+FDA +DA+
Sbjct: 189 EFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDAL 248

Query: 254 YSALEKSGGGGLEIVVSETGWPT----DRGVAANVDNARTYINNLIQHV--KVGTPKRPR 313
           Y AL       ++I+V+ETGWPT        AA+ DNA TY +N+I+HV    GTP +P 
Sbjct: 249 YYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPG 308

Query: 314 RPVETYVFAMFDENEKTTPPEVEKHWGLFSPNKQPKYHVNF 344
             +  Y+F++F+EN K    + E++WGLF P++   Y ++F
Sbjct: 309 EAMNVYIFSLFNENRK-AGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of Lag0035271 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961079.1	6.5e-158	81.16	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata]	[more]
XP_022987103.1	2.1e-156	80.58	probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima]	[more]
XP_022139185.1	4.4e-154	79.71	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]
KAG6590085.1	3.0e-147	81.06	Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma...	[more]
XP_038895085.1	4.4e-146	76.59	glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida]	[more]

BLAST of Lag0035271 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	2.2e-116	63.35	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
Q9M2M0	3.0e-113	60.67	Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q03773	1.7e-108	59.31	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
P36401	9.0e-102	56.29	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]
P07979	2.2e-100	55.29	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]

BLAST of Lag0035271 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HCY3	3.1e-158	81.16	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P...	[more]
A0A6J1JIH6	1.0e-156	80.58	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=...	[more]
A0A6J1CBL7	2.1e-154	79.71	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ...	[more]
A0A1S3C9Z3	2.5e-139	74.64	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV...	[more]
A0A0A0LYQ8	4.3e-139	73.99	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3...	[more]

BLAST of Lag0035271 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57270.1	2.1e-114	60.67	beta-1,3-glucanase 1	[more]
AT3G57240.1	1.1e-99	55.49	beta-1,3-glucanase 3	[more]
AT3G57260.1	6.2e-98	54.36	beta-1,3-glucanase 2	[more]
AT4G16260.1	7.3e-91	49.56	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	1.6e-66	39.30	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 31..344 e-value: 9.2E-131 score: 437.5
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 27..341
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 33..343 e-value: 9.6E-121 score: 403.2
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 27..341
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 33..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035271.1	Lag0035271.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds