Lag0035162 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035162
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Polyubiquitin
Location	chr3: 15972540 .. 15972953 (-)
RNA-Seq Expression	Lag0035162
Synteny	Lag0035162

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAATCTTCGTCAAAACCCTCACAGGCAAGACCATCACCCTCGAGGTGGAGTCTTCTGATACCATCGTCAAAGTCAAGGCCAAGATCCAGGATAAGGAAGGAATCCCTCCGGATCAACAACGTTTGATCTTCGCCGGCAAGCAGCTTGAGGATGGCCGAACCCTTGCTGACTACAACATTCAGAAGGAGTCAACTCTCCACCTCGTCCTAAGGCTTCGTGGTGGCATGCAAATTTTCGTCAAGACCCTTACCGGTAAGACAATCACCCTTGAGGTGGAGTCTTCCGACACCATTGACAACGTAAAGGCCAAGATCCAGGACAAGGAAGGAATTCCCTCGGACCAGCAACGTCTGATCTTCGCTGGAAAGCAACTTGAGAACGGTCGAACCCTTGCCGACTACAACATCTAG

mRNA sequence

ATGCAAATCTTCGTCAAAACCCTCACAGGCAAGACCATCACCCTCGAGGTGGAGTCTTCTGATACCATCGTCAAAGTCAAGGCCAAGATCCAGGATAAGGAAGGAATCCCTCCGGATCAACAACGTTTGATCTTCGCCGGCAAGCAGCTTGAGGATGGCCGAACCCTTGCTGACTACAACATTCAGAAGGAGTCAACTCTCCACCTCGTCCTAAGGCTTCGTGGTGGCATGCAAATTTTCGTCAAGACCCTTACCGGTAAGACAATCACCCTTGAGGTGGAGTCTTCCGACACCATTGACAACGTAAAGGCCAAGATCCAGGACAAGGAAGGAATTCCCTCGGACCAGCAACGTCTGATCTTCGCTGGAAAGCAACTTGAGAACGGTCGAACCCTTGCCGACTACAACATCTAG

Coding sequence (CDS)

ATGCAAATCTTCGTCAAAACCCTCACAGGCAAGACCATCACCCTCGAGGTGGAGTCTTCTGATACCATCGTCAAAGTCAAGGCCAAGATCCAGGATAAGGAAGGAATCCCTCCGGATCAACAACGTTTGATCTTCGCCGGCAAGCAGCTTGAGGATGGCCGAACCCTTGCTGACTACAACATTCAGAAGGAGTCAACTCTCCACCTCGTCCTAAGGCTTCGTGGTGGCATGCAAATTTTCGTCAAGACCCTTACCGGTAAGACAATCACCCTTGAGGTGGAGTCTTCCGACACCATTGACAACGTAAAGGCCAAGATCCAGGACAAGGAAGGAATTCCCTCGGACCAGCAACGTCTGATCTTCGCTGGAAAGCAACTTGAGAACGGTCGAACCCTTGCCGACTACAACATCTAG

Protein sequence

MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLIFAGKQLENGRTLADYNI

Homology

[Close]

BLAST of Lag0035162 vs. NCBI nr
Match: XP_039785111.1 (polyubiquitin-like [Panicum virgatum] >KAG2656044.1 hypothetical protein PVAP13_1KG050331 [Panicum virgatum])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 134/137 (97.81%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 77  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 136

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 137 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 196

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLENGRTLADYNI
Sbjct: 197 FAGKQLENGRTLADYNI 213

[Close]

BLAST of Lag0035162 vs. NCBI nr
Match: VAI55726.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 134/137 (97.81%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI
Sbjct: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 121 FAGKQLEDGRTLADYNI 137

[Close]

BLAST of Lag0035162 vs. NCBI nr
Match: NP_001353310.1 (polyubiquitin [Solanum lycopersicum])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 134/137 (97.81%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 212

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI
Sbjct: 213 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 272

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 273 FAGKQLEDGRTLADYNI 289

[Close]

BLAST of Lag0035162 vs. NCBI nr
Match: VAI55746.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 134/137 (97.81%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 77  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 136

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI
Sbjct: 137 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 196

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 197 FAGKQLEDGRTLADYNI 213

[Close]

BLAST of Lag0035162 vs. NCBI nr
Match: KAB2044267.1 (hypothetical protein ES319_D01G076900v1 [Gossypium barbadense] >PPD89931.1 hypothetical protein GOBAR_DD13135 [Gossypium barbadense] >TYG82406.1 hypothetical protein ES288_D01G085000v1 [Gossypium darwinii])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 134/137 (97.81%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI KVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 288

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 289 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 348

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 349 FAGKQLEDGRTLADYNI 365

[Close]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot
Match: P0CG85 (Polyubiquitin OS=Nicotiana sylvestris OX=4096 GN=UBI11 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 8.3e-67
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 120

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 121 FAGKQLEDGRTLADYNI 137

[Close]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot
Match: P0CH04 (Polyubiquitin OS=Petroselinum crispum OX=4043 GN=PCUBI4-1 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 8.3e-67
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 120

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 121 FAGKQLEDGRTLADYNI 137

[Close]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot
Match: P0CH05 (Polyubiquitin OS=Petroselinum crispum OX=4043 GN=PCUBI4-2 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 8.3e-67
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 120

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 121 FAGKQLEDGRTLADYNI 137

[Close]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot
Match: P0CG84 (Polyubiquitin (Fragment) OS=Nicotiana sylvestris OX=4096 GN=UBI4 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 8.3e-67
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 73  MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 132

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 133 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 192

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 193 FAGKQLEDGRTLADYNI 209

[Close]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot
Match: P69309 (Polyubiquitin OS=Avena fatua OX=4499 PE=2 SV=2)

HSP 1 Score: 254.2 bits (648), Expect = 8.3e-67
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 1   MQIFVKTLTGKTITLEVESSDTIVKVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60
           MQIFVKTLTGKTITLEVESSDTI  VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPSDQQRLI 120
           IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIP DQQRLI
Sbjct: 61  IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI 120

Query: 121 FAGKQLENGRTLADYNI 138
           FAGKQLE+GRTLADYNI
Sbjct: 121 FAGKQLEDGRTLADYNI 137

The following BLAST results are available for this feature:

BLAST of Lag0035162 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_039785111.1	1.7e-64	97.81	polyubiquitin-like [Panicum virgatum] >KAG2656044.1 hypothetical protein PVAP13_...	[more]
VAI55726.1	1.7e-64	97.81	unnamed protein product [Triticum turgidum subsp. durum]	[more]
NP_001353310.1	1.7e-64	97.81	polyubiquitin [Solanum lycopersicum]	[more]
VAI55746.1	1.7e-64	97.81	unnamed protein product [Triticum turgidum subsp. durum]	[more]
KAB2044267.1	1.7e-64	97.81	hypothetical protein ES319_D01G076900v1 [Gossypium barbadense] >PPD89931.1 hypot...	[more]

BLAST of Lag0035162 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CG85	8.3e-67	97.08	Polyubiquitin OS=Nicotiana sylvestris OX=4096 GN=UBI11 PE=2 SV=1	[more]
P0CH04	8.3e-67	97.08	Polyubiquitin OS=Petroselinum crispum OX=4043 GN=PCUBI4-1 PE=2 SV=1	[more]
P0CH05	8.3e-67	97.08	Polyubiquitin OS=Petroselinum crispum OX=4043 GN=PCUBI4-2 PE=3 SV=1	[more]
P0CG84	8.3e-67	97.08	Polyubiquitin (Fragment) OS=Nicotiana sylvestris OX=4096 GN=UBI4 PE=2 SV=1	[more]
P69309	8.3e-67	97.08	Polyubiquitin OS=Avena fatua OX=4499 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019956	Ubiquitin domain	PRINTS	PR00348	UBIQUITIN	coord: 11..31 score: 78.97 coord: 32..52 score: 95.05 coord: 53..74 score: 91.18
IPR000626	Ubiquitin-like domain	SMART	SM00213	ubq_7	coord: 1..72 e-value: 2.7E-34 score: 129.9 coord: 77..137 e-value: 2.4E-19 score: 80.3
IPR000626	Ubiquitin-like domain	PFAM	PF00240	ubiquitin	coord: 79..137 e-value: 7.9E-26 score: 89.6 coord: 3..74 e-value: 4.1E-33 score: 113.0
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 1..76 score: 30.212576
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 77..137 score: 24.159016
None	No IPR available	GENE3D	3.10.20.90		coord: 78..137 e-value: 8.3E-35 score: 120.5
None	No IPR available	GENE3D	3.10.20.90		coord: 1..77 e-value: 2.4E-46 score: 158.0
None	No IPR available	PANTHER	PTHR10666	UBIQUITIN	coord: 1..137
None	No IPR available	PANTHER	PTHR10666:SF364	SUBFAMILY NOT NAMED	coord: 1..137
None	No IPR available	CDD	cd01803	Ubl_ubiquitin	coord: 1..76 e-value: 3.54158E-49 score: 150.287
IPR019954	Ubiquitin conserved site	PROSITE	PS00299	UBIQUITIN_1	coord: 27..52
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 76..137
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 1..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035162.1	Lag0035162.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019941	modification-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0031386	protein tag
molecular_function	GO:0031625	ubiquitin protein ligase binding
molecular_function	GO:0005515	protein binding