Lag0035024 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0035024
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	WAT1-related protein
Location	chr3: 13729475 .. 13731932 (-)
RNA-Seq Expression	Lag0035024
Synteny	Lag0035024

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGACTTTTCTGTTTCTGCTTAGAAATGTGGTCGCATTTCATTATGCCTTGGCAGAAATGTGATCGCATTTCTACTGTTTGCCTTGTTTTCCTCTATTTTTTGCAACACGTAAAAATAAGTTACATTCCTACCAAAATGAGTTCATTTAAGCACAAATATGCATAATTCAATACAAATGACTCTCTAAGATTCAACTTACCAACTTTTACACTTTTTAGGTTCGAGAATAGTAAATTTGACCAAAAATATACAAGTTACAAAAGAAATAAGCTAAGAATAAGGGGCAAAATACGTATATTTTTTACACTTAATCAATGCTCATGTTGGCAATCCACGTCTTTTTGAGGTTTAGGGCAATAGTAATTCCTATCTTTACATTTTTAATTTTATATTCTAAAATTATAGGTGCTAAATTATAGGTTACCACTACAACAAATTATAGTTTTAATGTCGGACAGAAAAATAGGCGCGGAGGCTTTAATGTCGGACAAAAAAATGCGCGGGCCTTTTAATGTCGGACGCGCGTTTTTTAAAATTTTCGAACTTTTAATGTCGGGCATTGCCCGACATCAAAAGACGCCTTTAATGTCGGGCATTGCCCGACATTAAAAGATATTTTAATTTTTTTTTAAATTTTAAAAACAGTAAAAAGGTAAAGTATTTCTCTCTTCGTTTTTCCCTCTCTCTTCGAAAACAAACCCAAACCCTCGGCGCCGCTTCCTCCCTCGCAGCACATTTCCTCCCTCATCGACGGCGCTTCCTCCCTCACCGACGGCAACCCTCGACGACGAGCTCACCGACGGCGTCCGAAGATTTCTCCCTCACCGACGTCGACTGTCTTCTCGCGTGCGTGAGTTTCTTTGCTTGAACCTTTCTCTATCGTCGGCCACAATAAAGAGAAACTTCTGCTTCAACCAAATGGAAACATTGCTTCTGCTATAAAATGTTCCAATTAAATCTTAATCAAACATGTTGTGGCCCTGAATAACCTGTTCAATTACAGGTTTCCCTAGCCCAGCTCTGGATGTGGGGGTTTCAACACCAGCTCCACAAGCAGTCCATGCAAAGAAATTGGTTCTGTTGTGGTTTCTTCAGGTTCCAAGTCAGAGAATTCTGTCAAATTTTATCTAGTTTTAAATATTACCCTGTGTGCAATGATGGTTGAAAGAATGATATTTTGGATTGTTGAATATTAAACTGGATGTTGAGTTCATTATGCCCCTGTACTGTTTGCTGAAATGCCTCACAGGAGAAGTTTAAGCTGATTTCTTCAATTTCAACCATTTTGGGATTATGTAAATGGGAAAAAAATGAAGAAGATTTCAATCTTTCTCTTTGAATTTGACAAGGTTGATCAACATATTTCTCAATCCATTATAAAAGCATGCTGAAACTTCTGCTTTTGTTGTGATTTATCTATTCTATTGACAAACACTTCTAGAGAGAGAGAGAAATGGAACAGGCAGGACATATGTTTGAGAAGGCAAAGCCATATTTGGGAGTAATTGCACTAAGATTTGCTTCTGCAGGAATGTCTATTATCTCTAACGCAAAGGCAGGACATTGTTGTTGCTCCTTTTGCCTTTGTCTTGGACAGGTACTAATTTTCTCTCGCTCTAAGCTATATTTTGTCTAGCCTTTCATCATCACTAACCCATTTCATTGGCAGAAAAATAAGGCGAAAGATGACTCTTCGAATTTTCGCCAAGATTCTTCTACTGGGTTTACTCGAGTAAGAACTTAACCCCCTTCGTCATCTTTACAAAAAAATTATTCAACATGATCACCACAATGTGACTGTCCCACATAACTATTATAGTGAAAATATTGTCTTTTATATATTTCACTTTGCATATATTTTGACAGTATAAAATCGTTAGTTATATCGGTTCAAAAACTAACCTTCTTTTCATTCTGACAGGCCTGTAATAGCCCAGAGCTTGATTTACTCTGGTACTAAATACACAACTGCAACTTTTGCAACTGCCATGTGCAATATTCTTCCTGCCTTTGCGTTTCTAATGGCTTGGATTTGCATGTACTTGTTTTGTATACCAACTTAAATTAAACGCACTCATGCACATAGAATAAGATCCTAATGTTTTCTGTTTGTTTATTGATTCAAGATCATCATTATGTTTTTGTTTTTGAAGAATGGAGAAGGTGAATATTAAAAGTTTACGAAGCCAGGCAAAGATTTTAGGCACTTTAGTTACAGTAGGAGGAGCAATGATGATGGCTCTTATAAAAGGACCCATGTTGAGTTTGCCTTGGACCAAAGAGAATTTCAACCCTCACTCTTATTCTAACATTGTAAATAAGTAAGAACCTGTTAAGGCTGCCATTGTTATCACCATTTCAAGTATCGTTTATTACTAAATCTAAAGGCCATGTCATTTGCAGGGCATAGTATATCTTCTGGGATTGCCTATTATCTCCAAGGAATTGTGGTGA

mRNA sequence

ATGCAGACTTTTCTGTTTCTGCTTAGAAATGTGGTCGCATTTCATTATGCCTTGGCAGAAATCACATTTCCTCCCTCATCGACGGCGCTTCCTCCCTCACCGACGGCAACCCTCGACGACGAGCTCACCGACGGCGTCCGAAGATTTCTCCCTCACCGACGTCGACTGTCTTCTCGCGTGCGTTTCCCTAGCCCAGCTCTGGATGTGGGGGTTTCAACACCAGCTCCACAAGCAGTCCATGCAAAGAAATTGGTTCTGTTGTGGTTTCTTCAGGAATGTCTATTATCTCTAACGCAAAGGCAGGACATTGTTGTTGCTCCTTTTGCCTTTGTCTTGGACAGAAAAATAAGGCGAAAGATGACTCTTCGAATTTTCGCCAAGATTCTTCTACTGGGTTTACTCGAGCCTGTAATAGCCCAGAGCTTGATTTACTCTGGTACTAAATACACAACTGCAACTTTTGCAACTGCCATGTGCAATATTCTTCCTGCCTTTGCGTTTCTAATGGCTTGGATTTGCATAATGGAGAAGGTGAATATTAAAAGTTTACGAAGCCAGGCAAAGATTTTAGGCACTTTAGTTACAGTAGGAGGAGCAATGATGATGGCTCTTATAAAAGGACCCATGTTGAGTTTGCCTTGGACCAAAGAGAATTTCAACCCTCACTCTTATTCTAACATTGGCATAGTATATCTTCTGGGATTGCCTATTATCTCCAAGGAATTGTGGTGA

Coding sequence (CDS)

ATGCAGACTTTTCTGTTTCTGCTTAGAAATGTGGTCGCATTTCATTATGCCTTGGCAGAAATCACATTTCCTCCCTCATCGACGGCGCTTCCTCCCTCACCGACGGCAACCCTCGACGACGAGCTCACCGACGGCGTCCGAAGATTTCTCCCTCACCGACGTCGACTGTCTTCTCGCGTGCGTTTCCCTAGCCCAGCTCTGGATGTGGGGGTTTCAACACCAGCTCCACAAGCAGTCCATGCAAAGAAATTGGTTCTGTTGTGGTTTCTTCAGGAATGTCTATTATCTCTAACGCAAAGGCAGGACATTGTTGTTGCTCCTTTTGCCTTTGTCTTGGACAGAAAAATAAGGCGAAAGATGACTCTTCGAATTTTCGCCAAGATTCTTCTACTGGGTTTACTCGAGCCTGTAATAGCCCAGAGCTTGATTTACTCTGGTACTAAATACACAACTGCAACTTTTGCAACTGCCATGTGCAATATTCTTCCTGCCTTTGCGTTTCTAATGGCTTGGATTTGCATAATGGAGAAGGTGAATATTAAAAGTTTACGAAGCCAGGCAAAGATTTTAGGCACTTTAGTTACAGTAGGAGGAGCAATGATGATGGCTCTTATAAAAGGACCCATGTTGAGTTTGCCTTGGACCAAAGAGAATTTCAACCCTCACTCTTATTCTAACATTGGCATAGTATATCTTCTGGGATTGCCTATTATCTCCAAGGAATTGTGGTGA

Protein sequence

MQTFLFLLRNVVAFHYALAEITFPPSSTALPPSPTATLDDELTDGVRRFLPHRRRLSSRVRFPSPALDVGVSTPAPQAVHAKKLVLLWFLQECLLSLTQRQDIVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNILPAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPHSYSNIGIVYLLGLPIISKELW

Homology

[Close]

BLAST of Lag0035024 vs. NCBI nr
Match: KAG6578719.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 223.0 bits (567), Expect = 2.8e-54
Identity = 114/126 (90.48%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+LSLPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLSLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. NCBI nr
Match: XP_022992772.1 (WAT1-related protein At2g39510-like [Cucurbita maxima] >XP_022992773.1 WAT1-related protein At2g39510-like [Cucurbita maxima])

HSP 1 Score: 223.0 bits (567), Expect = 2.8e-54
Identity = 114/126 (90.48%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+LSLPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLSLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. NCBI nr
Match: XP_023549874.1 (WAT1-related protein At2g39510-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.9 bits (564), Expect = 6.2e-54
Identity = 113/126 (89.68%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+L+LPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLNLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. NCBI nr
Match: XP_022938758.1 (WAT1-related protein At2g39510-like [Cucurbita moschata] >XP_022938759.1 WAT1-related protein At2g39510-like [Cucurbita moschata])

HSP 1 Score: 221.9 bits (564), Expect = 6.2e-54
Identity = 113/126 (89.68%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIY+GTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYAGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+LSLPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLSLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. NCBI nr
Match: KAG6602358.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.2 bits (557), Expect = 4.0e-53
Identity = 114/125 (91.20%), Postives = 117/125 (93.60%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRKIR KMTLRIF KILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 48  IVVAPFAFVLDRKIRPKMTLRIFGKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 107

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAFLMAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGPML+LPWTKEN  PH
Sbjct: 108 PAFAFLMAWICRMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPMLNLPWTKENHIPH 167

Query: 223 SYSNI 228
           SYS +
Sbjct: 168 SYSTL 172

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BLAST of Lag0035024 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.3e-38
Identity = 81/127 (63.78%), Postives = 99/127 (77.95%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           I +APFA+ LDRKIR KMTL IF KILLLGLLEP I Q+L Y+G KYT+ATF  AM N+L
Sbjct: 49  IFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVL 108

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWI  +EKVN+K + SQAKILGT+VTVGGAM+M ++KGP++ LPW   +    
Sbjct: 109 PAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQ 168

Query: 223 SYSNIGI 230
             SN G+
Sbjct: 169 DSSNTGV 175

[Close]

BLAST of Lag0035024 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-32
Identity = 73/114 (64.04%), Postives = 91/114 (79.82%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IV+APFAF  D+K+R KMTL IF KI LLGLLEPVI Q+L Y G KYTTATFATAM N+L
Sbjct: 56  IVMAPFAFYFDKKVRPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVL 115

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTK 217
           PA  F++A+I  +E+V ++ +RS  K++GTL TVGGAM+M L+KGP+L L WTK
Sbjct: 116 PAITFVLAYIFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTK 169

[Close]

BLAST of Lag0035024 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.7e-28
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           +V+APFA + +RK+R KMTL +  KI+ LG LEPV+ Q   Y G   T+AT+ +A+ NIL
Sbjct: 54  LVLAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFN 221
           P+  F++AWI  MEKVNI  +RS+AKI+GTLV +GGA++M L KGP++ LPW+  N +
Sbjct: 114 PSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMD 171

[Close]

BLAST of Lag0035024 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.9e-27
Identity = 59/124 (47.58%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 104 VVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNILP 163
           ++ PFA +L+R  R K+T +I  +I +L L EPV+ Q+L YSG K TTATF +A+CN LP
Sbjct: 50  LITPFALILERNTRPKLTFKILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALP 109

Query: 164 AFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKEN--FNP 223
           A  F+MA +  +EKV I+   SQAK++GT+V +GGAM+M  +KG ++ LPWT  +   N 
Sbjct: 110 AMTFIMACVFKLEKVTIERRHSQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNG 169

Query: 224 HSYS 226
           H+++
Sbjct: 170 HTHA 173

[Close]

BLAST of Lag0035024 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.2e-25
Identity = 61/110 (55.45%), Postives = 81/110 (73.64%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IV+APFA +L+RKIR KMT  +F +IL LG LEP++ Q+L Y G K T+AT+++A  N L
Sbjct: 52  IVIAPFALILERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNAL 111

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSL 213
           PA  F+MA I  +E VN+K  RS AK++GT +TVGGAM+M L KGP + L
Sbjct: 112 PAITFIMAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIEL 161

[Close]

BLAST of Lag0035024 vs. ExPASy TrEMBL
Match: A0A6J1K073 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111489010 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 114/126 (90.48%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+LSLPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLSLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. ExPASy TrEMBL
Match: A0A6J1FF17 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111444884 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 3.0e-54
Identity = 113/126 (89.68%), Postives = 120/126 (95.24%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRK+R KMTL IFAKILLLGLLEPVIAQSLIY+GTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKVRPKMTLPIFAKILLLGLLEPVIAQSLIYAGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGP+LSLPWTKEN NPH
Sbjct: 114 PAFAFIMAWICKMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPLLSLPWTKENSNPH 173

Query: 223 SYSNIG 229
           SYS++G
Sbjct: 174 SYSSLG 179

[Close]

BLAST of Lag0035024 vs. ExPASy TrEMBL
Match: A0A6J1HAM4 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462214 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.9e-53
Identity = 114/125 (91.20%), Postives = 117/125 (93.60%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRKIR KMTLRIF KILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKIRPKMTLRIFGKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAFLMAWIC MEKVNI+ LRSQAKILGTLVTVGGAMMM LIKGPML+LPWTKEN  PH
Sbjct: 114 PAFAFLMAWICRMEKVNIRRLRSQAKILGTLVTVGGAMMMTLIKGPMLNLPWTKENHIPH 173

Query: 223 SYSNI 228
           SYS +
Sbjct: 174 SYSTL 178

[Close]

BLAST of Lag0035024 vs. ExPASy TrEMBL
Match: A0A6J1JRX2 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111487257 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 5.6e-53
Identity = 113/125 (90.40%), Postives = 116/125 (92.80%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRKIR KMTLRIF KILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKIRPKMTLRIFGKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAFLMAWIC MEKVNI+  RSQAKILGTLVTVGGAMMM LIKGPML+LPWTKEN  PH
Sbjct: 114 PAFAFLMAWICRMEKVNIRRFRSQAKILGTLVTVGGAMMMTLIKGPMLNLPWTKENHIPH 173

Query: 223 SYSNI 228
           SYS +
Sbjct: 174 SYSTL 178

[Close]

BLAST of Lag0035024 vs. ExPASy TrEMBL
Match: A0A5D3C601 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003170 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 5.6e-53
Identity = 114/126 (90.48%), Postives = 119/126 (94.44%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IVVAPFAFVLDRKIR KMTL IFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL
Sbjct: 54  IVVAPFAFVLDRKIRPKMTLPIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKE-NFNP 222
           PAFAFLMAWIC MEKVNI+SLRSQAKILGTLVTVGGAMMM L+KGP+LSLPWT + NFNP
Sbjct: 114 PAFAFLMAWICRMEKVNIRSLRSQAKILGTLVTVGGAMMMTLLKGPLLSLPWTNQNNFNP 173

Query: 223 HSYSNI 228
           HSYS +
Sbjct: 174 HSYSTL 179

[Close]

BLAST of Lag0035024 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 161.4 bits (407), Expect = 9.2e-40
Identity = 81/127 (63.78%), Postives = 99/127 (77.95%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           I +APFA+ LDRKIR KMTL IF KILLLGLLEP I Q+L Y+G KYT+ATF  AM N+L
Sbjct: 49  IFIAPFAYFLDRKIRPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVL 108

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFNPH 222
           PAFAF+MAWI  +EKVN+K + SQAKILGT+VTVGGAM+M ++KGP++ LPW   +    
Sbjct: 109 PAFAFIMAWIFRLEKVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQ 168

Query: 223 SYSNIGI 230
             SN G+
Sbjct: 169 DSSNTGV 175

[Close]

BLAST of Lag0035024 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 141.4 bits (355), Expect = 9.9e-34
Identity = 73/114 (64.04%), Postives = 91/114 (79.82%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           IV+APFAF  D+K+R KMTL IF KI LLGLLEPVI Q+L Y G KYTTATFATAM N+L
Sbjct: 56  IVMAPFAFYFDKKVRPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVL 115

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTK 217
           PA  F++A+I  +E+V ++ +RS  K++GTL TVGGAM+M L+KGP+L L WTK
Sbjct: 116 PAITFVLAYIFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTK 169

[Close]

BLAST of Lag0035024 vs. TAIR 10
Match: AT4G08290.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.9e-29
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           +V+APFA + +RK+R KMTL +  KI+ LG LEPV+ Q   Y G   T+AT+ +A+ NIL
Sbjct: 54  LVLAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFN 221
           P+  F++AWI  MEKVNI  +RS+AKI+GTLV +GGA++M L KGP++ LPW+  N +
Sbjct: 114 PSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMD 171

[Close]

BLAST of Lag0035024 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.9e-29
Identity = 62/118 (52.54%), Postives = 87/118 (73.73%), Query Frame = 0


Query: 103 IVVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNIL 162
           +V+APFA + +RK+R KMTL +  KI+ LG LEPV+ Q   Y G   T+AT+ +A+ NIL
Sbjct: 54  LVLAPFALIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNIL 113

Query: 163 PAFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKENFN 221
           P+  F++AWI  MEKVNI  +RS+AKI+GTLV +GGA++M L KGP++ LPW+  N +
Sbjct: 114 PSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMD 171

[Close]

BLAST of Lag0035024 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.8e-28
Identity = 59/124 (47.58%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 104 VVAPFAFVLDRKIRRKMTLRIFAKILLLGLLEPVIAQSLIYSGTKYTTATFATAMCNILP 163
           ++ PFA +L+R  R K+T +I  +I +L L EPV+ Q+L YSG K TTATF +A+CN LP
Sbjct: 50  LITPFALILERNTRPKLTFKILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALP 109

Query: 164 AFAFLMAWICIMEKVNIKSLRSQAKILGTLVTVGGAMMMALIKGPMLSLPWTKEN--FNP 223
           A  F+MA +  +EKV I+   SQAK++GT+V +GGAM+M  +KG ++ LPWT  +   N 
Sbjct: 110 AMTFIMACVFKLEKVTIERRHSQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNG 169

Query: 224 HSYS 226
           H+++
Sbjct: 170 HTHA 173

The following BLAST results are available for this feature:

BLAST of Lag0035024 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578719.1	2.8e-54	90.48	WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022992772.1	2.8e-54	90.48	WAT1-related protein At2g39510-like [Cucurbita maxima] >XP_022992773.1 WAT1-rela...	[more]
XP_023549874.1	6.2e-54	89.68	WAT1-related protein At2g39510-like [Cucurbita pepo subsp. pepo]	[more]
XP_022938758.1	6.2e-54	89.68	WAT1-related protein At2g39510-like [Cucurbita moschata] >XP_022938759.1 WAT1-re...	[more]
KAG6602358.1	4.0e-53	91.20	WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0035024 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80638	1.3e-38	63.78	WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2...	[more]
Q9ZUS1	1.4e-32	64.04	WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2...	[more]
Q9SUF1	2.7e-28	52.54	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]
Q9FNA5	3.9e-27	47.58	WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2...	[more]
Q501F8	8.2e-25	55.45	WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2...	[more]

BLAST of Lag0035024 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K073	1.3e-54	90.48	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111489010 PE=3 SV=1	[more]
A0A6J1FF17	3.0e-54	89.68	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111444884 PE=3 SV=1	[more]
A0A6J1HAM4	1.9e-53	91.20	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462214 PE=3 SV=1	[more]
A0A6J1JRX2	5.6e-53	90.40	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111487257 PE=3 SV=1	[more]
A0A5D3C601	5.6e-53	90.48	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G...	[more]

BLAST of Lag0035024 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39510.1	9.2e-40	63.78	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G37460.1	9.9e-34	64.04	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G08290.2	1.9e-29	52.54	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G08290.1	1.9e-29	52.54	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G13670.1	2.8e-28	47.58	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 103..202 e-value: 1.7E-10 score: 41.2
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 103..219
None	No IPR available	PANTHER	PTHR31218:SF215	WAT1-RELATED PROTEIN	coord: 103..219
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 110..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0035024.1	Lag0035024.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity